Christian Metallo, PhD

Professor

Molecular and Cell Biology Laboratory

Christian Metallo
Salk Institute for Biological Studies - Publications

all Publications


Linares, J.F., Cid-Diaz, T., Duran, A., Osrodek, M., Martinez-Ordoñez, A., Reina-Campos, M., Kuo, H.H., Elemento, O., Martin, M.L., Cordes, T., Thompson, T.C., Metallo, C.M., Moscat, J., Diaz-Meco, M.T. The lactate-NAD axis activates cancer-associated fibroblasts by downregulating p62. (2022) Cell Reports. 39(6):110792. DOI: 10.1016/j.celrep.2022.110792

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Casanova-Vallve, N., Duglan, D., Vaughan, M.E., Pariollaud, M., Handzlik, M.K., Fan, W., Yu, R.T., Liddle, C., Downes, M., Delezie, J., Mello, R., Chan, A.B., Westermark, P.O., Metallo, C.M., Evans, R.M., Lamia, K.A. Daily running enhances molecular and physiological circadian rhythms in skeletal muscle. (2022) Molecular Metabolism.:101504 DOI: 10.1016/j.molmet.2022.101504

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Parekh, U., McDonald, D., Dailamy, A., Wu, Y., Cordes, T., Zhang, K., Tipps, A., Metallo, C., Mali, P. Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas. (2021) iScience. 24(10):103149. DOI: 10.1016/j.isci.2021.103149

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Lim, E.W., Handzlik, M.K., Trefts, E., Gengatharan, J.M., Pondevida, C.M., Shaw, R.J., Metallo, C.M. Progressive alterations in amino acid and lipid metabolism correlate with peripheral neuropathy in mice. (2021) Science Advances. 7(42):eabj4077. DOI: 10.1126/sciadv.abj4077

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Kumar, A., Cordes, T., Thalacker-Mercer, A.E., Pajor, A.M., Murphy, A.N., Metallo, C.M. NaCT/SLC13A5 facilitates citrate import and metabolism under nutrient-limited conditions. (2021) Cell Reports. 36(11):109701. DOI: 10.1016/j.celrep.2021.109701

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Yebra, M., Bhargava, S., Kumar, A., Burgoyne, A.M., Tang, C.M., Yoon, H., Banerjee, S., Aguilera, J., Cordes, T., Sheth, V., Noh, S., Ustoy, R., Li, S., Advani, S.J., Corless, C.L., Heinrich, M.C., Kurzrock, R., Lippman, S.M., Fanta, P.T., Harismendy, O., Metallo, C., Sicklick, J.K. Establishment of Patient-derived Succinate Dehydrogenase-deficient Gastrointestinal Stromal Tumor Models For Predicting Therapeutic Response. (2021) Clinical Cancer Research. DOI: 10.1158/1078-0432.CCR-21-2092

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Ravi, A., Palamiuc, L., Loughran, R.M., Triscott, J., Arora, G.K., Kumar, A., Tieu, V., Pauli, C., Reist, M., Lew, R.J., Houlihan, S.L., Fellmann, C., Metallo, C., Rubin, M.A., Emerling, B.M. PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay. (2021) Developmental Cell. 56(11):1661-1676.e10. DOI: 10.1016/j.devcel.2021.04.019

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Su, H., Yang, F., Fu, R., Li, X., French, R., Mose, E., Pu, X., Trinh, B., Kumar, A., Liu, J., Antonucci, L., Todoric, J., Liu, Y., Hu, Y., Diaz-Meco, M.T., Moscat, J., Metallo, C.M., Lowy, A.M., Sun, B., Karin, M. Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis. (2021) Cancer Cell. 39(5):678-693.e11. DOI: 10.1016/j.ccell.2021.02.016

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Sawh, M.C., Wallace, M., Shapiro, E., Goyal, N.P., Newton, K.P., Yu, E.L., Bross, C., Durelle, J., Knott, C., Gangoiti, J.A., Barshop, B.A., Gengatharan, J.M., Meurs, N., Schlein, A., Middleton, M.S., Sirlin, C.B., Metallo, C.M., Schwimmer, J.B. Dairy Fat Intake, Plasma Pentadecanoic Acid, and Plasma Iso-heptadecanoic Acid Are Inversely Associated With Liver Fat in Children. (2021) J Pediatr Gastroenterol Nutr. 72(4):e90-e96. DOI: 10.1097/MPG.0000000000003040

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Cordes, T., Metallo, C.M. Exploring the evolutionary roots and physiological function of itaconate. (2021) Curr Opin Biotechnol. 68:144-150. DOI: 10.1016/j.copbio.2020.11.005

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Eade, K., Gantner, M.L., Hostyk, J.A., Nagasaki, T., Giles, S., Fallon, R., Harkins-Perry, S., Baldini, M., Lim, E.W., Scheppke, L., Dorrell, M.I., Cai, C., Baugh, E.H., Wolock, C.J., Wallace, M., Berlow, R.B., Goldstein, D.B., Metallo, C.M., Friedlander, M., Allikmets, R. Serine biosynthesis defect due to haploinsufficiency of PHGDH causes retinal disease. (2021) Nat Metab. 3(3):366-377. DOI: 10.1038/s42255-021-00361-3

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Cordes, T., Metallo, C.M. Itaconate Alters Succinate and Coenzyme A Metabolism via Inhibition of Mitochondrial Complex II and Methylmalonyl-CoA Mutase. (2021) Metabolites. 11(2). DOI: 10.3390/metabo11020117

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Lee, Y.M., Mu, A., Wallace, M., Gengatharan, J.M., Furst, A.J., Bode, L., Metallo, C.M., Ayres, J.S. Microbiota control of maternal behavior regulates early postnatal growth of offspring. (2021) Science Advances. 7(5). DOI: 10.1126/sciadv.abe6563

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Gheller, B.J., Blum, J.E., Lim, E.W., Handzlik, M.K., Hannah Fong, E.H., Ko, A.C., Khanna, S., Gheller, M.E., Bender, E.L., Alexander, M.S., Stover, P.J., Field, M.S., Cosgrove, B.D., Metallo, C.M., Thalacker-Mercer, A.E. Extracellular serine and glycine are required for mouse and human skeletal muscle stem and progenitor cell function. (2021) Molecular Metabolism. 43:101106. DOI: 10.1016/j.molmet.2020.101106

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Huh, J.Y., Reilly, S.M., Abu-Odeh, M., Murphy, A.N., Mahata, S.K., Zhang, J., Cho, Y., Seo, J.B., Hung, C.W., Green, C.R., Metallo, C.M., Saltiel, A.R. TANK-Binding Kinase 1 Regulates the Localization of Acyl-CoA Synthetase ACSL1 to Control Hepatic Fatty Acid Oxidation. (2020) Cell Metabolism. 32(6):1012-1027.e7. DOI: 10.1016/j.cmet.2020.10.010

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Wallace, M., Metallo, C.M. Tracing insights into de novo lipogenesis in liver and adipose tissues. (2020) Seminars in Cell & Developmental Biology. 108:65-71. DOI: 10.1016/j.semcdb.2020.02.012

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Gheller, M.E., Vermeylen, F., Handzlik, M.K., Gheller, B.J., Bender, E., Metallo, C., Aydemir, T.B., Smriga, M., Thalacker-Mercer, A.E. Tolerance to graded dosages of histidine supplementation in healthy human adults. (2020) Am J Clin Nutr. 112(5):1358-1367. DOI: 10.1093/ajcn/nqaa210

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Rudd, A.K., Mittal, N., Lim, E.W., Metallo, C.M., Devaraj, N.K. A Small Molecule Fluorogenic Probe for the Detection of Sphingosine in Living Cells. (2020) Journal of the American Chemical Society. 142(42):17887-17891. DOI: 10.1021/jacs.0c06652

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Todoric, J., Di Caro, G., Reibe, S., Henstridge, D.C., Green, C.R., Vrbanac, A., Ceteci, F., Conche, C., McNulty, R., Shalapour, S., Taniguchi, K., Meikle, P.J., Watrous, J.D., Moranchel, R., Najhawan, M., Jain, M., Liu, X., Kisseleva, T., Diaz-Meco, M.T., Moscat, J., Knight, R., Greten, F.R., Lau, L.F., Metallo, C.M., Febbraio, M.A., Karin, M. Fructose stimulated de novo lipogenesis is promoted by inflammation. (2020) Nat Metab. 2(10):1034-1045. DOI: 10.1038/s42255-020-0261-2

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Muthusamy, T., Cordes, T., Handzlik, M.K., You, L., Lim, E.W., Gengatharan, J., Pinto, A.F.M., Badur, M.G., Kolar, M.J., Wallace, M., Saghatelian, A., Metallo, C.M. Serine restriction alters sphingolipid diversity to constrain tumour growth. (2020) Nature. 586(7831):790-795. DOI: 10.1038/s41586-020-2609-x

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Henke, C., Töllner, K., van Dijk, R.M., Miljanovic, N., Cordes, T., Twele, F., Bröer, S., Ziesak, V., Rohde, M., Hauck, S.M., Vogel, C., Welzel, L., Schumann, T., Willmes, D.M., Kurzbach, A., El-Agroudy, N.N., Bornstein, S.R., Schneider, S.A., Jordan, J., Potschka, H., Metallo, C.M., Köhling, R., Birkenfeld, A.L., Löscher, W. Disruption of the sodium-dependent citrate transporter SLC13A5 in mice causes alterations in brain citrate levels and neuronal network excitability in the hippocampus. (2020) Neurobiol Dis. 143:105018. DOI: 10.1016/j.nbd.2020.105018

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Gaglio, D., Bonanomi, M., Valtorta, S., Bharat, R., Ripamonti, M., Conte, F., Fiscon, G., Righi, N., Napodano, E., Papa, F., Raccagni, I., Parker, S.J., Cifola, I., Camboni, T., Paci, P., Colangelo, A.M., Vanoni, M., Metallo, C.M., Moresco, R.M., Alberghina, L. Disruption of redox homeostasis for combinatorial drug efficacy in tumors as revealed by metabolic connectivity profiling. (2020) Cancer Metab. 8:22. DOI: 10.1186/s40170-020-00227-4


Kudo, Y., Sugimoto, M., Arias, E., Kasashima, H., Cordes, T., Linares, J.F., Duran, A., Nakanishi, Y., Nakanishi, N., L'Hermitte, A., Campos, A., Senni, N., Rooslid, T., Roberts, L.R., Cuervo, A.M., Metallo, C.M., Karin, M., Diaz-Meco, M.T., Moscat, J. PKCλ/ι Loss Induces Autophagy, Oxidative Phosphorylation, and NRF2 to Promote Liver Cancer Progression. (2020) Cancer Cell. 38(2):247-262.e11. DOI: 10.1016/j.ccell.2020.05.018

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Feyen, D.A.M., McKeithan, W.L., Bruyneel, A.A.N., Spiering, S., Hörmann, L., Ulmer, B., Zhang, H., Briganti, F., Schweizer, M., Hegyi, B., Liao, Z., Pölönen, R.P., Ginsburg, K.S., Lam, C.K., Serrano, R., Wahlquist, C., Kreymerman, A., Vu, M., Amatya, P.L., Behrens, C.S., Ranjbarvaziri, S., Maas, R.G.C., Greenhaw, M., Bernstein, D., Wu, J.C., Bers, D.M., Eschenhagen, T., Metallo, C.M., Mercola, M. Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived Cardiomyocytes. (2020) Cell Reports. 32(3):107925. DOI: 10.1016/j.celrep.2020.107925

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Kumar, A., Mitchener, J., King, Z.A., Metallo, C.M. Escher-Trace: a web application for pathway-based visualization of stable isotope tracing data. (2020) BMC Bioinformatics. 21(1):297. DOI: 10.1186/s12859-020-03632-0

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Vaughan, M.E., Wallace, M., Handzlik, M.K., Chan, A.B., Metallo, C.M., Lamia, K.A. Cryptochromes Suppress HIF1α in Muscles. (2020) iScience. 23(7):101338. DOI: 10.1016/j.isci.2020.101338

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Hong, S., Dechaumphai, E., Green, C.R., Lal, R., Murphy, A.N., Metallo, C.M., Chen, R. Sub-nanowatt microfluidic single-cell calorimetry. (2020) Nature Communications. 11(1):2982. DOI: 10.1038/s41467-020-16697-5

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Liu, X., Olszewski, K., Zhang, Y., Lim, E.W., Shi, J., Zhang, X., Zhang, J., Lee, H., Koppula, P., Lei, G., Zhuang, L., You, M.J., Fang, B., Li, W., Metallo, C.M., Poyurovsky, M.V., Gan, B. Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. (2020) Nature Cell Biology. 22(4):476-486. DOI: 10.1038/s41556-020-0496-x

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Cordes, T., Lucas, A., Divakaruni, A.S., Murphy, A.N., Cabrales, P., Metallo, C.M. Itaconate modulates tricarboxylic acid and redox metabolism to mitigate reperfusion injury. (2020) Molecular Metabolism. 32:122-135. DOI: 10.1016/j.molmet.2019.11.019

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Cordes, T., Metallo, C.M. Statins Limit Coenzyme Q Synthesis and Metabolically Synergize with MEK Inhibition in Pancreatic Tumors. (2020) Cancer Research. 80(2):151-152. DOI: 10.1158/0008-5472.CAN-19-3415

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Lim, E.W., Parker, S.J., Metallo, C.M. Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cells. (2020) Methods in Molecular Biology. 2088:51-71. DOI: 10.1007/978-1-0716-0159-4_4

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Gao, X., Zhao, L., Liu, S., Li, Y., Xia, S., Chen, D., Wang, M., Wu, S., Dai, Q., Vu, H., Zacharias, L., DeBerardinis, R., Lim, E., Metallo, C., Boggon, T.J., Lonial, S., Lin, R., Mao, H., Pan, Y., Shan, C., Chen, J. γ-6-Phosphogluconolactone, a Byproduct of the Oxidative Pentose Phosphate Pathway, Contributes to AMPK Activation through Inhibition of PP2A. (2019) Molecular Cell. 76(6):857-871.e9. DOI: 10.1016/j.molcel.2019.09.007

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Gantner, M.L., Eade, K., Wallace, M., Handzlik, M.K., Fallon, R., Trombley, J., Bonelli, R., Giles, S., Harkins-Perry, S., Heeren, T.F.C., Sauer, L., Ideguchi, Y., Baldini, M., Scheppke, L., Dorrell, M.I., Kitano, M., Hart, B.J., Cai, C., Nagasaki, T., Badur, M.G., Okada, M., Woods, S.M., Egan, C., Gillies, M., Guymer, R., Eichler, F., Bahlo, M., Fruttiger, M., Allikmets, R., Bernstein, P.S., Metallo, C.M., Friedlander, M. Serine and Lipid Metabolism in Macular Disease and Peripheral Neuropathy. (2019) New England Journal of Medicine. 381(15):1422-1433. DOI: 10.1056/NEJMoa1815111

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Jung, S.M., Hung, C.M., Hildebrand, S.R., Sanchez-Gurmaches, J., Martinez-Pastor, B., Gengatharan, J.M., Wallace, M., Mukhopadhyay, D., Martinez Calejman, C., Luciano, A.K., Hsiao, W.Y., Tang, Y., Li, H., Daniels, D.L., Mostoslavsky, R., Metallo, C.M., Guertin, D.A. Non-canonical mTORC2 Signaling Regulates Brown Adipocyte Lipid Catabolism through SIRT6-FoxO1. (2019) Molecular Cell. 75(4):807-822.e8. DOI: 10.1016/j.molcel.2019.07.023

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Ramms, B., Patel, S., Nora, C., Pessentheiner, A.R., Chang, M.W., Green, C.R., Golden, G.J., Secrest, P., Krauss, R.M., Metallo, C.M., Benner, C., Alexander, V.J., Witztum, J.L., Tsimikas, S., Esko, J.D., Gordts, P.L.S.M. ApoC-III ASO promotes tissue LPL activity in the absence of apoE-mediated TRL clearance. (2019) Journal of Lipid Research. 60(8):1379-1395. DOI: 10.1194/jlr.M093740

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Velez, D.O., Ranamukhaarachchi, S.K., Kumar, A., Modi, R.N., Lim, E.W., Engler, A.J., Metallo, C.M., Fraley, S.I. 3D collagen architecture regulates cell adhesion through degradability, thereby controlling metabolic and oxidative stress. (2019) Integr Biol (Camb). 11(5):221-234. DOI: 10.1093/intbio/zyz019

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Reina-Campos, M., Linares, J.F., Duran, A., Cordes, T., L'Hermitte, A., Badur, M.G., Bhangoo, M.S., Thorson, P.K., Richards, A., Rooslid, T., Garcia-Olmo, D.C., Nam-Cha, S.Y., Salinas-Sanchez, A.S., Eng, K., Beltran, H., Scott, D.A., Metallo, C.M., Moscat, J., Diaz-Meco, M.T. Increased Serine and One-Carbon Pathway Metabolism by PKCλ/ι Deficiency Promotes Neuroendocrine Prostate Cancer. (2019) Cancer Cell. 35(3):385-400.e9. DOI: 10.1016/j.ccell.2019.01.018

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Garcia, D., Hellberg, K., Chaix, A., Wallace, M., Herzig, S., Badur, M.G., Lin, T., Shokhirev, M.N., Pinto, A.F.M., Ross, D.S., Saghatelian, A., Panda, S., Dow, L.E., Metallo, C.M., Shaw, R.J. Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. (2019) Cell Reports. 26(1):192-208.e6. DOI: 10.1016/j.celrep.2018.12.036

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Cordes, T., Metallo, C.M. Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry. (2019) Methods in Molecular Biology. 1978:219-241. DOI: 10.1007/978-1-4939-9236-2_14

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Wortham, M., Benthuysen, J.R., Wallace, M., Savas, J.N., Mulas, F., Divakaruni, A.S., Liu, F., Albert, V., Taylor, B.L., Sui, Y., Saez, E., Murphy, A.N., Yates, J.R., Metallo, C.M., Sander, M. Integrated In Vivo Quantitative Proteomics and Nutrient Tracing Reveals Age-Related Metabolic Rewiring of Pancreatic β Cell Function. (2018) Cell Reports. 25(10):2904-2918.e8. DOI: 10.1016/j.celrep.2018.11.031

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Badur, M.G., Muthusamy, T., Parker, S.J., Ma, S., McBrayer, S.K., Cordes, T., Magana, J.H., Guan, K.L., Metallo, C.M. Oncogenic R132 IDH1 Mutations Limit NADPH for De Novo Lipogenesis through (D)2-Hydroxyglutarate Production in Fibrosarcoma Cells. (2018) Cell Reports. 25(6):1680. DOI: 10.1016/j.celrep.2018.10.099


Wallace, M., Green, C.R., Roberts, L.S., Lee, Y.M., McCarville, J.L., Sanchez-Gurmaches, J., Meurs, N., Gengatharan, J.M., Hover, J.D., Phillips, S.A., Ciaraldi, T.P., Guertin, D.A., Cabrales, P., Ayres, J.S., Nomura, D.K., Loomba, R., Metallo, C.M. Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues. (2018) Nature Chemical Biology. 14(11):1021-1031. DOI: 10.1038/s41589-018-0132-2

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Badur, M.G., Muthusamy, T., Parker, S.J., Ma, S., McBrayer, S.K., Cordes, T., Magana, J.H., Guan, K.L., Metallo, C.M. Oncogenic R132 IDH1 Mutations Limit NADPH for De Novo Lipogenesis through (D)2-Hydroxyglutarate Production in Fibrosarcoma Sells. (2018) Cell Reports. 25(4):1018-1026.e4. DOI: 10.1016/j.celrep.2018.09.074

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McBrayer, S.K., Mayers, J.R., DiNatale, G.J., Shi, D.D., Khanal, J., Chakraborty, A.A., Sarosiek, K.A., Briggs, K.J., Robbins, A.K., Sewastianik, T., Shareef, S.J., Olenchock, B.A., Parker, S.J., Tateishi, K., Spinelli, J.B., Islam, M., Haigis, M.C., Looper, R.E., Ligon, K.L., Bernstein, B.E., Carrasco, R.D., Cahill, D.P., Asara, J.M., Metallo, C.M., Yennawar, N.H., Vander Heiden, M.G., Kaelin, W.G. Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. (2018) Cell. 175(1):101-116.e25. DOI: 10.1016/j.cell.2018.08.038

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Sessions, A.O., Min, P., Cordes, T., Weickert, B.J., Divakaruni, A.S., Murphy, A.N., Metallo, C.M., Engler, A.J. Preserved cardiac function by vinculin enhances glucose oxidation and extends health- and life-span. (2018) APL Bioeng. 2(3). DOI: 10.1063/1.5019592

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Skorobogatko, Y., Dragan, M., Cordon, C., Reilly, S.M., Hung, C.W., Xia, W., Zhao, P., Wallace, M., Lackey, D.E., Chen, X.W., Osborn, O., Bogner-Strauss, J.G., Theodorescu, D., Metallo, C.M., Olefsky, J.M., Saltiel, A.R. RalA controls glucose homeostasis by regulating glucose uptake in brown fat. (2018) Proceedings of the National Academy of Sciences of the United States of America. 115(30):7819-7824. DOI: 10.1073/pnas.1801050115

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Zhao, D., Badur, M.G., Luebeck, J., Magaña, J.H., Birmingham, A., Sasik, R., Ahn, C.S., Ideker, T., Metallo, C.M., Mali, P. Combinatorial CRISPR-Cas9 Metabolic Screens Reveal Critical Redox Control Points Dependent on the KEAP1-NRF2 Regulatory Axis. (2018) Molecular Cell. 69(4):699-708.e7. DOI: 10.1016/j.molcel.2018.01.017

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Badur, M.G., Metallo, C.M. Reverse engineering the cancer metabolic network using flux analysis to understand drivers of human disease. (2018) Metab Eng. 45:95-108. DOI: 10.1016/j.ymben.2017.11.013

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Jin, L., Chun, J., Pan, C., Kumar, A., Zhang, G., Ha, Y., Li, D., Alesi, G.N., Kang, Y., Zhou, L., Yu, W.M., Magliocca, K.R., Khuri, F.R., Qu, C.K., Metallo, C., Owonikoko, T.K., Kang, S. The PLAG1-GDH1 Axis Promotes Anoikis Resistance and Tumor Metastasis through CamKK2-AMPK Signaling in LKB1-Deficient Lung Cancer. (2018) Molecular Cell. 69(1):87-99.e7. DOI: 10.1016/j.molcel.2017.11.025

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Linares, J.F., Cordes, T., Duran, A., Reina-Campos, M., Valencia, T., Ahn, C.S., Castilla, E.A., Moscat, J., Metallo, C.M., Diaz-Meco, M.T. ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma. (2017) Cell Metabolism. 26(6):817-829.e6. DOI: 10.1016/j.cmet.2017.09.001

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Parker, S.J., Svensson, R.U., Divakaruni, A.S., Lefebvre, A.E., Murphy, A.N., Shaw, R.J., Metallo, C.M. LKB1 promotes metabolic flexibility in response to energy stress. (2017) Metab Eng. 43(Pt B):208-217. DOI: 10.1016/j.ymben.2016.12.010

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Metallo, C.M., Deberardinis, R.J. Engineering approaches to study cancer metabolism. (2017) Metab Eng. 43(Pt B):93. DOI: 10.1016/j.ymben.2017.09.002


Zarei, M., Lal, S., Parker, S.J., Nevler, A., Vaziri-Gohar, A., Dukleska, K., Mambelli-Lisboa, N.C., Moffat, C., Blanco, F.F., Chand, S.N., Jimbo, M., Cozzitorto, J.A., Jiang, W., Yeo, C.J., Londin, E.R., Seifert, E.L., Metallo, C.M., Brody, J.R., Winter, J.M. Posttranscriptional Upregulation of IDH1 by HuR Establishes a Powerful Survival Phenotype in Pancreatic Cancer Cells. (2017) Cancer Research. 77(16):4460-4471. DOI: 10.1158/0008-5472.CAN-17-0015

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Divakaruni, A.S., Wallace, M., Buren, C., Martyniuk, K., Andreyev, A.Y., Li, E., Fields, J.A., Cordes, T., Reynolds, I.J., Bloodgood, B.L., Raymond, L.A., Metallo, C.M., Murphy, A.N. Inhibition of the mitochondrial pyruvate carrier protects from excitotoxic neuronal death. (2017) Journal of Cell Biology. 216(4):1091-1105. DOI: 10.1083/jcb.201612067

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Cordes, T., Metallo, C.M. Tracing insights into human metabolism using chemical engineering approaches. (2016) Curr Opin Chem Eng. 14:72-81. DOI: 10.1016/j.coche.2016.08.019

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Zhao, S., Torres, A., Henry, R.A., Trefely, S., Wallace, M., Lee, J.V., Carrer, A., Sengupta, A., Campbell, S.L., Kuo, Y.M., Frey, A.J., Meurs, N., Viola, J.M., Blair, I.A., Weljie, A.M., Metallo, C.M., Snyder, N.W., Andrews, A.J., Wellen, K.E. ATP-Citrate Lyase Controls a Glucose-to-Acetate Metabolic Switch. (2016) Cell Reports. 17(4):1037-1052. DOI: 10.1016/j.celrep.2016.09.069

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Svensson, R.U., Parker, S.J., Eichner, L.J., Kolar, M.J., Wallace, M., Brun, S.N., Lombardo, P.S., Van Nostrand, J.L., Hutchins, A., Vera, L., Gerken, L., Greenwood, J., Bhat, S., Harriman, G., Westlin, W.F., Harwood, H.J., Saghatelian, A., Kapeller, R., Metallo, C.M., Shaw, R.J. Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models. (2016) Nature Medicine. 22(10):1108-1119. DOI: 10.1038/nm.4181

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Huber, A.L., Papp, S.J., Chan, A.B., Henriksson, E., Jordan, S.D., Kriebs, A., Nguyen, M., Wallace, M., Li, Z., Metallo, C.M., Lamia, K.A. CRY2 and FBXL3 Cooperatively Degrade c-MYC. (2016) Molecular Cell. 64(4):774-789. DOI: 10.1016/j.molcel.2016.10.012

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Zhang, H., Badur, M.G., Divakaruni, A.S., Parker, S.J., Jäger, C., Hiller, K., Murphy, A.N., Metallo, C.M. Distinct Metabolic States Can Support Self-Renewal and Lipogenesis in Human Pluripotent Stem Cells under Different Culture Conditions. (2016) Cell Reports. 16(6):1536-1547. DOI: 10.1016/j.celrep.2016.06.102

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Cordes, T., Wallace, M., Michelucci, A., Divakaruni, A.S., Sapcariu, S.C., Sousa, C., Koseki, H., Cabrales, P., Murphy, A.N., Hiller, K., Metallo, C.M. Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Intracellular Succinate Levels. (2016) Journal of Biological Chemistry. 291(27):14274-14284. DOI: 10.1074/jbc.M115.685792

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Jiang, L., Shestov, A.A., Swain, P., Yang, C., Parker, S.J., Wang, Q.A., Terada, L.S., Adams, N.D., McCabe, M.T., Pietrak, B., Schmidt, S., Metallo, C.M., Dranka, B.P., Schwartz, B., DeBerardinis, R.J. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. (2016) Nature. 532(7598):255-8. DOI: 10.1038/nature17393

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Parker, S.J., Metallo, C.M. Chasing One-Carbon Units to Understand the Role of Serine in Epigenetics. (2016) Molecular Cell. 61(2):185-6. DOI: 10.1016/j.molcel.2016.01.006

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Green, C.R., Wallace, M., Divakaruni, A.S., Phillips, S.A., Murphy, A.N., Ciaraldi, T.P., Metallo, C.M. Branched-chain amino acid catabolism fuels adipocyte differentiation and lipogenesis. (2016) Nature Chemical Biology. 12(1):15-21. DOI: 10.1038/nchembio.1961

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Lussey-Lepoutre, C., Hollinshead, K.E., Ludwig, C., Menara, M., Morin, A., Castro-Vega, L.J., Parker, S.J., Janin, M., Martinelli, C., Ottolenghi, C., Metallo, C., Gimenez-Roqueplo, A.P., Favier, J., Tennant, D.A. Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. (2015) Nature Communications. 6:8784. DOI: 10.1038/ncomms9784

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Badur, M.G., Zhang, H., Metallo, C.M. Enzymatic passaging of human embryonic stem cells alters central carbon metabolism and glycan abundance. (2015) Biotechnol J. 10(10):1600-11. DOI: 10.1002/biot.201400749

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Parker, S.J., Metallo, C.M. Metabolic consequences of oncogenic IDH mutations. (2015) Pharmacol Ther. 152:54-62. DOI: 10.1016/j.pharmthera.2015.05.003

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Buescher, J.M., Antoniewicz, M.R., Boros, L.G., Burgess, S.C., Brunengraber, H., Clish, C.B., DeBerardinis, R.J., Feron, O., Frezza, C., Ghesquiere, B., Gottlieb, E., Hiller, K., Jones, R.G., Kamphorst, J.J., Kibbey, R.G., Kimmelman, A.C., Locasale, J.W., Lunt, S.Y., Maddocks, O.D., Malloy, C., Metallo, C.M., Meuillet, E.J., Munger, J., Nöh, K., Rabinowitz, J.D., Ralser, M., Sauer, U., Stephanopoulos, G., St-Pierre, J., Tennant, D.A., Wittmann, C., Vander Heiden, M.G., Vazquez, A., Vousden, K., Young, J.D., Zamboni, N., Fendt, S.M. A roadmap for interpreting (13)C metabolite labeling patterns from cells. (2015) Curr Opin Biotechnol. 34:189-201. DOI: 10.1016/j.copbio.2015.02.003

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Metallo, C.M. Metabolic Analysis Using Stable Isotopes. Preface. (2015) Methods Enzymol. 561:xv-xvi. DOI: 10.1016/S0076-6879(15)00482-6


Ahn, C.S., Metallo, C.M. Mitochondria as biosynthetic factories for cancer proliferation. (2015) Cancer Metab. 3(1):1. DOI: 10.1186/s40170-015-0128-2

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Vacanti, N.M., Divakaruni, A.S., Green, C.R., Parker, S.J., Henry, R.R., Ciaraldi, T.P., Murphy, A.N., Metallo, C.M. Regulation of substrate utilization by the mitochondrial pyruvate carrier. (2014) Molecular Cell. 56(3):425-435. DOI: 10.1016/j.molcel.2014.09.024

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Sourjik, V., Metallo, C. Editorial overview: Systems biology: Advances in multi-scale systems biology applications. (2014) Curr Opin Biotechnol. 28:vi-viii. DOI: 10.1016/j.copbio.2014.06.016


Valencia, T., Kim, J.Y., Abu-Baker, S., Moscat-Pardos, J., Ahn, C.S., Reina-Campos, M., Duran, A., Castilla, E.A., Metallo, C.M., Diaz-Meco, M.T., Moscat, J. Metabolic reprogramming of stromal fibroblasts through p62-mTORC1 signaling promotes inflammation and tumorigenesis. (2014) Cancer Cell. 26(1):121-135. DOI: 10.1016/j.ccr.2014.05.004

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Lewis, C.A., Parker, S.J., Fiske, B.P., McCloskey, D., Gui, D.Y., Green, C.R., Vokes, N.I., Feist, A.M., Vander Heiden, M.G., Metallo, C.M. Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. (2014) Molecular Cell. 55(2):253-63. DOI: 10.1016/j.molcel.2014.05.008

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Heinrichsen, E.T., Zhang, H., Robinson, J.E., Ngo, J., Diop, S., Bodmer, R., Joiner, W.J., Metallo, C.M., Haddad, G.G. Metabolic and transcriptional response to a high-fat diet in Drosophila melanogaster. (2014) Molecular Metabolism. 3(1):42-54. DOI: 10.1016/j.molmet.2013.10.003

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Commisso, C., Davidson, S.M., Soydaner-Azeloglu, R.G., Parker, S.J., Kamphorst, J.J., Hackett, S., Grabocka, E., Nofal, M., Drebin, J.A., Thompson, C.B., Rabinowitz, J.D., Metallo, C.M., Vander Heiden, M.G., Bar-Sagi, D. Macropinocytosis of protein is an amino acid supply route in Ras-transformed cells. (2013) Nature. 497(7451):633-7. DOI: 10.1038/nature12138

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Hiller, K., Wegner, A., Weindl, D., Cordes, T., Metallo, C.M., Kelleher, J.K., Stephanopoulos, G. NTFD--a stand-alone application for the non-targeted detection of stable isotope-labeled compounds in GC/MS data. (2013) Bioinformatics . 29(9):1226-8. DOI: 10.1093/bioinformatics/btt119

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Metallo, C.M., Vander Heiden, M.G. Understanding metabolic regulation and its influence on cell physiology. (2013) Molecular Cell. 49(3):388-98. DOI: 10.1016/j.molcel.2013.01.018

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Hiller, K., Metallo, C.M. Profiling metabolic networks to study cancer metabolism. (2013) Curr Opin Biotechnol. 24(1):60-8. DOI: 10.1016/j.copbio.2012.11.001

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Vacanti, N.M., Metallo, C.M. Exploring metabolic pathways that contribute to the stem cell phenotype. (2013) Biochimica et Biophysica Acta. 1830(2):2361-9. DOI: 10.1016/j.bbagen.2012.08.007

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Metallo, C.M. Expanding the reach of cancer metabolomics. (2012) Cancer Prevention Research. 5(12):1337-40. DOI: 10.1158/1940-6207.CAPR-12-0433

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Anastasiou, D., Yu, Y., Israelsen, W.J., Jiang, J.K., Boxer, M.B., Hong, B.S., Tempel, W., Dimov, S., Shen, M., Jha, A., Yang, H., Mattaini, K.R., Metallo, C.M., Fiske, B.P., Courtney, K.D., Malstrom, S., Khan, T.M., Kung, C., Skoumbourdis, A.P., Veith, H., Southall, N., Walsh, M.J., Brimacombe, K.R., Leister, W., Lunt, S.Y., Johnson, Z.R., Yen, K.E., Kunii, K., Davidson, S.M., Christofk, H.R., Austin, C.P., Inglese, J., Harris, M.H., Asara, J.M., Stephanopoulos, G., Salituro, F.G., Jin, S., Dang, L., Auld, D.S., Park, H.W., Cantley, L.C., Thomas, C.J., Vander Heiden, M.G. Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis. (2012) Nature Chemical Biology. 8(10):839-47. DOI: 10.1038/nchembio.1060

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Walther, J.L., Metallo, C.M., Zhang, J., Stephanopoulos, G. Optimization of 13C isotopic tracers for metabolic flux analysis in mammalian cells. (2012) Metab Eng. 14(2):162-71. DOI: 10.1016/j.ymben.2011.12.004

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Metallo, C.M., Gameiro, P.A., Bell, E.L., Mattaini, K.R., Yang, J., Hiller, K., Jewell, C.M., Johnson, Z.R., Irvine, D.J., Guarente, L., Kelleher, J.K., Vander Heiden, M.G., Iliopoulos, O., Stephanopoulos, G. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. (2011) Nature. 481(7381):380-4. DOI: 10.1038/nature10602

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Gaglio, D., Metallo, C.M., Gameiro, P.A., Hiller, K., Danna, L.S., Balestrieri, C., Alberghina, L., Stephanopoulos, G., Chiaradonna, F. Oncogenic K-Ras decouples glucose and glutamine metabolism to support cancer cell growth. (2011) Mol Syst Biol. 7:523. DOI: 10.1038/msb.2011.56

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Grassian, A.R., Metallo, C.M., Coloff, J.L., Stephanopoulos, G., Brugge, J.S. Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation. (2011) Genes & Development. 25(16):1716-33. DOI: 10.1101/gad.16771811

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Locasale, J.W., Grassian, A.R., Melman, T., Lyssiotis, C.A., Mattaini, K.R., Bass, A.J., Heffron, G., Metallo, C.M., Muranen, T., Sharfi, H., Sasaki, A.T., Anastasiou, D., Mullarky, E., Vokes, N.I., Sasaki, M., Beroukhim, R., Stephanopoulos, G., Ligon, A.H., Meyerson, M., Richardson, A.L., Chin, L., Wagner, G., Asara, J.M., Brugge, J.S., Cantley, L.C., Vander Heiden, M.G. Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis. (2011) Nature Genetics. 43(9):869-74. DOI: 10.1038/ng.890

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Hiller, K., Metallo, C., Stephanopoulos, G. Elucidation of cellular metabolism via metabolomics and stable-isotope assisted metabolomics. (2011) Curr Pharm Biotechnol. 12(7):1075-86. DOI: 10.2174/138920111795909096

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Vander Heiden, M.G., Lunt, S.Y., Dayton, T.L., Fiske, B.P., Israelsen, W.J., Mattaini, K.R., Vokes, N.I., Stephanopoulos, G., Cantley, L.C., Metallo, C.M., Locasale, J.W. Metabolic pathway alterations that support cell proliferation. (2011) Cold Spring Harbor Symposia on Quantitative Biology. 76:325-34. DOI: 10.1101/sqb.2012.76.010900

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Metallo, C.M., Vander Heiden, M.G. Metabolism strikes back: metabolic flux regulates cell signaling. (2010) Genes & Development. 24(24):2717-22. DOI: 10.1101/gad.2010510

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Hiller, K., Metallo, C.M., Kelleher, J.K., Stephanopoulos, G. Nontargeted elucidation of metabolic pathways using stable-isotope tracers and mass spectrometry. (2010) Analytical Chemistry. 82(15):6621-8. DOI: 10.1021/ac1011574

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Metallo, C.M., Ji, L., de Pablo, J.J., Palecek, S.P. Directed differentiation of human embryonic stem cells to epidermal progenitors. (2010) Methods in Molecular Biology. 585:83-92. DOI: 10.1007/978-1-60761-380-0_7

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Metallo, C.M., Azarin, S.M., Moses, L.E., Ji, L., de Pablo, J.J., Palecek, S.P. Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs. (2010) Tissue Eng Part A. 16(1):213-23. DOI: 10.1089/ten.TEA.2009.0325

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Metallo, C.M., Walther, J.L., Stephanopoulos, G. Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. (2009) J Biotechnol. 144(3):167-74. DOI: 10.1016/j.jbiotec.2009.07.010

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Metallo, C.M., Vodyanik, M.A., de Pablo, J.J., Slukvin, I.I., Palecek, S.P. The response of human embryonic stem cell-derived endothelial cells to shear stress. (2008) Biotechnol Bioeng. 100(4):830-7. DOI: 10.1002/bit.21809

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Metallo, C.M., Azarin, S.M., Ji, L., de Pablo, J.J., Palecek, S.P. Engineering tissue from human embryonic stem cells. (2008) J Cell Mol Med. 12(3):709-29. DOI: 10.1111/j.1582-4934.2008.00228.x

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Metallo, C.M., Ji, L., de Pablo, J.J., Palecek, S.P. Retinoic acid and bone morphogenetic protein signaling synergize to efficiently direct epithelial differentiation of human embryonic stem cells. (2008) Stem Cells. 26(2):372-80. DOI: 10.1634/stemcells.2007-0501

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Metallo, C.M., Mohr, J.C., Detzel, C.J., de Pablo, J.J., Van Wie, B.J., Palecek, S.P. Engineering the stem cell microenvironment. (2007) Biotechnol Prog. 23(1):18-23. DOI: 10.1021/bp060350a

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Xie, L., Metallo, C., Warren, J., Pilbrough, W., Peltier, J., Zhong, T., Pikus, L., Yancy, A., Leung, J., Auniņs, J.G., Zhou, W. Large-scale propagation of a replication-defective adenovirus vector in stirred-tank bioreactor PER.C6 cell culture under sparging conditions. (2003) Biotechnol Bioeng. 83(1):45-52. DOI: 10.1002/bit.10644

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Xie, L., Pilbrough, W., Metallo, C., Zhong, T., Pikus, L., Leung, J., Auniņs, J.G., Zhou, W. Serum-free suspension cultivation of PER.C6(R) cells and recombinant adenovirus production under different pH conditions. (2002) Biotechnol Bioeng. 80(5):569-79. DOI: 10.1002/bit.10443

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Education

BS, Chemical Engineering, University of Pennsylvania
MS, Chemical and Biological Engineering, University of Wisconsin-Madison
PhD, Chemical and Biological Engineering, University of Wisconsin-Madison
Postdoctoral Fellow, Massachusetts Institute of Technology


Awards & Honors

  • Elected to AIMBE College of Fellows, 2021
  • Camille and Henry Dreyfus Teacher-Scholar Award, 2018
  • NSF CAREER Award, 2015
  • American Association of Clinical Chemistry Outstanding Speaker Award, 2015
  • Searle Scholar Award, 2013
  • Hellman Faculty Fellowship, Hellman Family Foundation, 2013
  • Rita Schaffer Young Investigator Award, BMES, 2012
  • American Cancer Society Postdoctoral Fellowship, 2009
  • NIH Biotechnology Graduate Fellowship, University of Wisconsin-Madison, 2004

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