Next Generation Sequencing Core

Scheduling & Rates

Salk Institute for Biological Studies - Next Generation Sequencing Core - Scheduling & Rates

Scheduling & Rates


Library Prep (Illumina Sequencing) Salk Rate Note
Stranded mRNA(PolyA+)-Seq Library Prep $ 144.00
Total RNA-Seq Library Prep $ 204.00
Small RNA Library Prep $ 205.00
Ultra Low input RNA-Seq cDNA Prep (SMART-seq V4) $ 146.00
Nextera XT/Flex DNA Library Prep $ 105.00
Single Cell RNA-Seq Library Prep: Full plate 96 well plate (SMART-seq V4 + Nextera XT) $ 8,228.00
ChIP-Seq library prep (Low-input, Swift) $ 135.00
ChIP-Seq library prep (NEB) $ 97.00
PCR-free sample Prep $ 105.00
10X Single-Cell RNA-Seq Library Prep (cDNA) $ 150.00
Illumina Sequencing Salk Rate Note
MiniSeq 75 Cycle (High-output) $ 991.00 Up to 25M reads
MiniSeq 150 Cycle (High-output) $ 1,145.00
MiniSeq 300 Cycle (High-output) $ 1,774.00
MiniSeq 300 Cycle (Mid-output) $ 694.00 Up to 7-8M reads
HiSeq 4000 SR50 (1 lane) $ 1,117.00 Per lane (8 lanes per flowcell), SR50 only
Up to ~350M reads
NextSeq 150 Cycle (Mid-output) $ 1,639.00 Up to ~130M reads
NextSeq 300 Cycle (Mid-output) $ 2,280.00 Up to ~130M reads
NextSeq 75 Cycle (High-output) $ 2,048.00 For PE42, single index only
Up to ~400M reads
NextSeq 150 Cycle (High-output) $ 3,367.00 Up to ~400M reads
NextSeq 300 Cycle (High-output) $ 5,025.00 Up to ~400M reads
NovaSeq SP 100 Cycle $ 3,826.00 Per full flowcell, ~0.8B reads
NovaSeq SP 300 Cycle $ 5,308.00
NovaSeq SP 500 Cycle $ 6,837.00
NovaSeq S1 100 Cycle $ 5,565.00 Per full flowcell, ~1.3-1.6B reads
NovaSeq S1 200 Cycle $ 6,862.00
NovaSeq S1 300 Cycle $ 7,882.00
NovaSeq S2 100 Cycle $ 10,584.00 Per full flowcell, ~3.3-4.1B reads
NovaSeq S2 200 Cycle $ 13,827.00
NovaSeq S2 300 Cycle $ 15,912.00
NovaSeq S4 200 Cycle $ 19,027.00 Per full flowcell, ~8-10B reads
NovaSeq S4 300 Cycle $ 21,577.00
XP workflow (2 lane) $ 347.00 For NovaSeq, SP, S1 and S2 flow cells
XP workflow (4 lane) $ 1,063.00 For NovaSeq, S4 flowcell only
10X Chromium Single Cell Solution Salk Rate Note
10X Chromium 3′ scRNA-Seq V3 Reagents $ 1,096.00 Until Current Supplies Last      
10X Chromium Chip B Single Cell Kit (3′ scRNA) $ 215.00
10X Chromium scATAC-seq Reagents $ 1,164.00
10X Chromium Chip E Single Cell Kit (scATAC) $ 247.00
10X Chromium 5′ scRNA-Seq Reagents $ 1,176.00
10X Chromium Chip A Single Cell Kit (5′ scRNA) $ 247.00
10X Chromium Next GEM Single Cell 3′ GEM v3.1 $ 1,196.00 Library & Gel Bead Kit, Per Sample
10X Chromium Next GEM Chip G Single Cell Kit $ 227.00 Per chip
10X Chromium Next GEM Single Cell ATAC v1.1 $ 1,141.00 Library & Gel Bead Kit, Per Sample
10X Chromium Next GEM Chip H Single Cell Kit $ 227.00 Per chip
Usage Fee $ 50.00 Per hour. This Includes Machine Usage, Workstation, all the nessasary equipments and consumables including index primers
Pacific Bioscience Sequel II Salk Rate (HHMI/UCSD) Note
Sequel II 8M SMRT cell (~15 hr movie) $ 2,622.00
Sequel II 8M SMRT cell (~30 hr movie) $ 2,891.00
HMW DNA Template Prep $ 517.00
HMW DNA Template Prep (Multiplexing) $ 528.00
HiFi Template Prep $ 669.00
Amplicon Template Prep $ 284.00 pool amplicons before submission
ISO Seq Template Prep $ 542.00
ISO-Seq cDNA multiplexing $ 198.00 per sample – barcoded cDNA sample will be pooled with the 1st sample
Sample QC HMW gDNA $ 75.00
Sample QC Amplicon, RNA, etc $ 20.00
Blue Pippin Size Selection $ 150.00
Oxford Nanopore Technology: GridION X5 Salk Rate Note
ONT GridIon X5 Flowcell (R9.4) $ 809.00
ONT GridIon X5 Machine Use Only $ 240.00 per flowcell
ONT HMW gDNA Library $ 292.00
ONT gDNA Library (10kb) $ 306.00
ONT Direct RNA library $ 350.00
ONT Direct cDNA library $ 350.00
ONT PCR-cDNA library $ 350.00
Quality Control (per sample) Salk Rate Note
Qubit Quantitation (self service) $ 3.00
Qubit Quantitation (full service) $ 5.00
Tape Station (self Service) $ 8.00 Will be charged at multples of 2
Tape Station (full Service) $ 15.00 Will be charged at multples of 2
Library Clean Up (Ampure beads) $ 25.00 Per sample
Library Clean Up (Gel) $ 55.00 Per sample
Library Pool qPCR Quants $ 30.00 Per sample
Library Pooling (include QC) $ 30.00 Per sample
Other Service Salk Rate Note
Covaris Sonication (Self service) $ 8.00
Covaris Sonication (Self service, Machine Use Only) $ 3.00
Epishear Sonication (Self service) $ 2.00
QuantStudio Q3 96 well plate $ 20.00
QuantStudio Q5 384 well plate $ 20.00
Tecan Plate Reader $ 10.00
3D Printer Usage* $ 5.00 *consumables and materials cost separate
DNA Mini Prep $ 2.50 Will be charged at multiples of 8 (1-8 samples will be $20)
Note:
ddPCR: Per Cartridges, gaskets will be move to Viral Vector Core (As of March 2020)
ddPCR Maching Use Only will be move to Viral Vector Core (As of March 2020)