Next Generation Sequencing Core

Scheduling & Rates

Salk Institute for Biological Studies - Next Generation Sequencing Core - Scheduling & Rates

Scheduling & Rates


Library Prep (Illumina Sequencing)Salk RateNote
Stranded mRNA(PolyA+)-Seq Library Prep$ 144.00
Total RNA-Seq Library Prep$ 204.00
Small RNA Library Prep$ 205.00
Ultra Low input RNA-Seq cDNA Prep (SMART-seq V4)$ 146.00
Nextera XT/Flex DNA Library Prep$ 105.00
Single Cell RNA-Seq Library Prep: Full plate 96 well plate (SMART-seq V4 + Nextera XT)$ 8,228.00
ChIP-Seq library prep (Low-input, Swift)$ 135.00
ChIP-Seq library prep (NEB)$ 97.00
PCR-free sample Prep$ 105.00
10X Single-Cell RNA-Seq Library Prep (cDNA)$ 150.00
Illumina SequencingSalk RateNote
MiniSeq 75 Cycle (High-output)$ 991.00 Up to 25M reads
MiniSeq 150 Cycle (High-output)$ 1,145.00
MiniSeq 300 Cycle (High-output)$ 1,774.00
MiniSeq 300 Cycle (Mid-output)$ 694.00Up to 7-8M reads
HiSeq 4000 SR50 (1 lane)$ 1,117.00Per lane (8 lanes per flowcell), SR50 only
Up to ~350M reads
NextSeq 150 Cycle (Mid-output)$ 1,639.00Up to ~130M reads
NextSeq 300 Cycle (Mid-output)$ 2,280.00Up to ~130M reads
NextSeq 75 Cycle (High-output)$ 2,048.00For PE42, single index only
Up to ~400M reads
NextSeq 150 Cycle (High-output)$ 3,367.00Up to ~400M reads
NextSeq 300 Cycle (High-output)$ 5,025.00Up to ~400M reads
NovaSeq SP 100 Cycle$ 3,826.00Per full flowcell, ~0.8B reads
NovaSeq SP 300 Cycle$ 5,308.00
NovaSeq SP 500 Cycle$ 6,837.00
NovaSeq S1 100 Cycle$ 5,565.00Per full flowcell, ~1.3-1.6B reads
NovaSeq S1 200 Cycle$ 6,862.00
NovaSeq S1 300 Cycle$ 7,882.00
NovaSeq S2 100 Cycle$ 10,584.00Per full flowcell, ~3.3-4.1B reads
NovaSeq S2 200 Cycle$ 13,827.00
NovaSeq S2 300 Cycle$ 15,912.00
NovaSeq S4 200 Cycle$ 19,027.00Per full flowcell, ~8-10B reads
NovaSeq S4 300 Cycle$ 21,577.00
XP workflow (2 lane)$ 347.00For NovaSeq, SP, S1 and S2 flow cells
XP workflow (4 lane)$ 1,063.00For NovaSeq, S4 flowcell only
10X Chromium Single Cell SolutionSalk RateNote
10X Chromium 3′ scRNA-Seq V3 Reagents$ 1,096.00Until Current Supplies Last   
10X Chromium Chip B Single Cell Kit (3′ scRNA)$ 215.00
10X Chromium scATAC-seq Reagents$ 1,164.00
10X Chromium Chip E Single Cell Kit (scATAC)$ 247.00
10X Chromium 5′ scRNA-Seq Reagents$ 1,176.00
10X Chromium Chip A Single Cell Kit (5′ scRNA)$ 247.00
10X Chromium Next GEM Single Cell 3′ GEM v3.1$ 1,196.00Library & Gel Bead Kit, Per Sample
10X Chromium Next GEM Chip G Single Cell Kit$ 227.00Per chip
10X Chromium Next GEM Single Cell ATAC v1.1$ 1,141.00Library & Gel Bead Kit, Per Sample
10X Chromium Next GEM Chip H Single Cell Kit$ 227.00Per chip
Usage Fee$ 50.00Per hour. This Includes Machine Usage, Workstation, all the nessasary equipments and consumables including index primers
Pacific Bioscience Sequel IISalk Rate (HHMI/UCSD)Note
Sequel II 8M SMRT cell (~15 hr movie)$ 2,622.00
Sequel II 8M SMRT cell (~30 hr movie)$ 2,891.00
HMW DNA Template Prep$ 517.00
HMW DNA Template Prep (Multiplexing)$ 528.00
HiFi Template Prep$ 669.00
Amplicon Template Prep$ 284.00pool amplicons before submission
ISO Seq Template Prep$ 542.00
ISO-Seq cDNA multiplexing$ 198.00per sample – barcoded cDNA sample will be pooled with the 1st sample
Sample QC HMW gDNA$ 75.00
Sample QC Amplicon, RNA, etc$ 20.00
Blue Pippin Size Selection$ 150.00
Oxford Nanopore Technology: GridION X5Salk RateNote
ONT GridIon X5 Flowcell (R9.4)$ 809.00
ONT GridIon X5 Machine Use Only$ 240.00per flowcell
ONT HMW gDNA Library$ 292.00
ONT gDNA Library (10kb)$ 306.00
ONT Direct RNA library$ 350.00
ONT Direct cDNA library$ 350.00
ONT PCR-cDNA library$ 350.00
Quality Control (per sample)Salk RateNote
Qubit Quantitation (self service)$ 3.00
Qubit Quantitation (full service)$ 5.00
Tape Station (self Service)$ 8.00Will be charged at multples of 2
Tape Station (full Service)$ 15.00Will be charged at multples of 2
Library Clean Up (Ampure beads)$ 25.00Per sample
Library Clean Up (Gel)$ 55.00Per sample
Library Pool qPCR Quants$ 30.00Per sample
Library Pooling (include QC)$ 30.00Per sample
Other ServiceSalk RateNote
Covaris Sonication (Self service)$ 8.00
Covaris Sonication (Self service, Machine Use Only)$ 3.00
Epishear Sonication (Self service)$ 2.00
QuantStudio Q3 96 well plate$ 20.00
QuantStudio Q5 384 well plate$ 20.00
Tecan Plate Reader$ 10.00
3D Printer Usage*$ 5.00*consumables and materials cost separate
DNA Mini Prep$ 2.50Will be charged at multiples of 8 (1-8 samples will be $20)
Note:
ddPCR: Per Cartridges, gaskets will be move to Viral Vector Core (As of March 2020)
ddPCR Maching Use Only will be move to Viral Vector Core (As of March 2020)