Next Generation Sequencing Core

Scheduling & Rates

Salk Institute for Biological Studies - Next Generation Sequencing Core - Scheduling & Rates

Scheduling & Rates


 

 

Suggested

Library Prep

Salk Price

Run Type

Reads (Millions)

Stranded mRNA-Seq

$140

1x50bp

20-30

Total RNA-Seq

$222

1x50bp

25-40

Small RNA-Seq

$194

1x50bp

5-10

Whole Genome

$101

2x125bp

30x coverage

Mouse Exome

Please Inquire

2x125bp

30x coverage

Human Exome

Please Inquire

2x125bp

30x coverage

ChIP-Seq (Low Input)

$86

1x50bp

10-40

ChIP-Seq

$80

1x50bp

10-40

ATAC-Seq

Please Inquire

 

15-40

GRO/PRO-Seq

Please Inquire

 

25-40

10X Genomics

Please Inquire

 

 

Nextera XT

Please Inquire

 

 

PacBio Library Preps

Please Inquire

 

 

Oxford Nanopore Library Preps

Please Inquire

 

 

For other library types, please contact: Nasun Hah nhah@salk.edu.

HiSeq 2500 (v4)

*~180-250 million reads/lane

Run type

Price Per Lane

1x 50 bp (SE50)

$1,088

1x 100 bp (SE100)

$1,438

2x 50 bp (PE50)

$1,726

2x 125 bp (PE125)

$2,443


NextSeq 500

Run type

Price Per Run

75 Cycle (High Output)

$2,051

150 Cycle (Mid Output)

$1,583

150 Cycle (High Output)

$3,376

300 Cycle (Mid Output)

$2,301

300 Cycle (High Output)

$5,140

*Mid Output: Total ~100-130 million reads
*High Output: Total ~300-400 million reads

For other run configurations, please contact: Nasun Hah nhah@salk.edu.

10X Genomic Single Cell Solution

Machine Usage Per Use

Please Inquire

Additional Cost Per Sample

Please Inquire

Library Prep per sample

Please Inquire

1.Researchers must supply their 10X Genomics Kits
2.See Service Section for Detail
3.Please inquire Salk Institute Quote

#For any inquiries regarding reagents, scheduling, pricing (Salk Institute Quote for 10X Genomics Kits) and 10X training request, please contact: Nasun Hah

Schedule 10X Genomics: Click here for Calendar

Please request access to calendar.

*PacBio Sequel (Long-read sequencing)

Sample preparation and validation

Please Inquire

SMRT Cell Sequencing

Please Inquire

*We are an HHMI-designated Pacific Biosciences sequencing site offering services for library preparation as well as sample sequencing. Salk, UCSD and HHMI Faculty receive preferred access and pricing. PacBio Sequencing services are made available to other external researchers as capacity permits. Please inquire.

Oxford Nanopore GridION X5 (Long-read sequencing)

Sample preparation and validation

Please Inquire

DNA/RNA Sequencing per flow cell

Please Inquire

RT-qPCR

per plate

QuantStudio Q3 96 well plate

$10

QuantStudio Q5 384 well plate

$10

#For any inquiries regarding scheduling, consumables and training request, please contact: Ling Ouyang.

Schedule RT-QPCR: Click here for Calendar

Please request access to calendar.

                                   

QX200 ddPCR

Machine Use Only

Machine Use & Reagents**

8 samples

$20

$50

Full plate discount

$220

$550

**QX200 ddPCR reagents include cartridges, gaskets, foil heat seals, semi-skirted 96-well plates, supermix (Evagreen or probes), droplet generation oil for Evagreen or probes, buffer control for Evagreen or probes (if needed), and droplet reader oil.

Other Services

Price Per Sample

Self Service

Full Service

Covaris Sonication

$5

$10

Epishear Sonication

$2

Agilent Tape Station

$7

$15

Qubit Quantitation

$2

$5

Library QC (Qubit and TapeStation)

 –

$20

Library Pooling†† (Includes QC)

 –

$30

Library qPCR Quants

 –

$20

Primer Dimer Cleanup

 –

$20

TECAN Micro Plate Reader*

 

3D-Printer (Form2)

$5^

DNA miniprep

 –

$1.50

††Library pooling requires core personnel to perform QC
* Machine use only. Researchers must supply their own reagents and plates.
^Researchers will pay consumables and materials cost in addition to printer use fee