Mass Spectrometry Core

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Salk Institute for Biological Studies - Mass Spectrometry Core - Resources

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Instrumentation

Thermo Orbitrap Fusion Tribrid Mass Spectrometer with EASY-nLC 1000 Liquid Chromatography System
The Thermo Orbitrap Fusion mass spectrometer combines its three mass analyzers -quadrupole, Orbitrap and linear ion trap in a Tribrid architecture that provides unprecedented depth of analysis for complex biological samples. It can perform a wide variety of analyses, from characterization of complex PTMs as well as comprehensive qualitative and quantitative analysis. Multiple fragmentation techniques (CID, HCD, and ETD)with fragment ion detection in either the ion trap or Orbitrap mass analyzer are available.

Thermo Q-Exactive Quadrupole-Obitrap Mass Spectrometer with EASY-nLC 1000 Liquid Chromatography System
The Thermo Q-Exactive mass spectrometer combines quadrupole precursor selection with high-resolution, accurate-mass Orbitrap detection. It generates high quality MS and MS/MS data. Its advanced quadrupole technology improves quantitation of low-abundance ions in complex matrices.

Thermo TSQ Quantiva Triple Quadrupole Mass Spectrometer with UltiMate 3000 Rapid Separation LC System
The TSQ Quantiva triple quadrupole mass spectrometer employs Active Ion Management (AIM) to optimize ion creation and transmission from the source to the detector, resulting in extreme sensitivity. This enhanced level of sensitivity greatly improves results in applications such as high-throughput targeted MS experiments and targeted quantitation of small molecules.

Thermo LTQ-Orbitrap XL

This hybrid instrument consists of a linear ion trap and an electrostatic Fourier transform analyzer and is equipped with a nano-spray source. The instrument affords high sensitivity, in the low femtomole range for peptides, and mass accuracies in the single ppm range. The instrument is used for proteomic analysis of proteins either separated by gel electrophoresis or by two-dimensional chromatography (MudPIT). Precursor masses are determined in the high resolution Orbitrap with <10ppm accuracy. Fragmentation is achieved by collisional or higher energy dissociation (CID or HCD). In addition to proteomic analyses, the instrument is capable of analyzing small intact proteins to obtain accurate masses.

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