Core Equipment List

+ Olympus FV1000MPE/CARS Two-Photon Laser Scanning Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with the following single-photon laser lines (405, 440, 488, 514, 559, 633nm) as well as a Spectra-Physics MaiTai DeepSee infrared laser for two-photon excitation. It is equipped with 3 internal descanned detectors as well as 4 non-descanned two-photon fluorescence detectors and 2 forward scanning fluorescence detectors for Second Harmonic Generation (SHG) and Coherent Anti-Stokes Raman Microscopy (CARS).
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss LSM 710 Laser Scanning Microscope (inverted platform) - Biophotonics Core

This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal detector for full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss CSU Spinning Disk Live Cell Confocal Microscope (inverted platform) - Biophotonics Core

This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and a Yokogawa spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Nikon TiE: PFS Live Cell TIRF Microscope (inverted platform) - Biophotonics Core

This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm), an Andor EM-CCD camera and the Nikon patented PerfectFocus module for TIRF imaging. It is also equipped with a stage-top incubation system for maintaining living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss VivaTiome Fluorescence Microscope (inverted platform) - Biophotonics Core

This inverted microscope is an advanced fluorescence microscope equipped with a multi-color LED illumination system and a filtered white light source as well as a high-resolution cooled CCD camera for fluorescence detection. The system is also equipped with a VivaTome unit, which is an aperture correlation based optical sectioning device capable of acquiring confocal images at near video-rate. The system is also equipped with a color CCD camera for visualizing histological samples.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Olympus BX61WI Live Cell Fluorescence Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with a DG-5 fast wavelength switcher for fluorescence excitation and a fast filter wheel for fluorescence emission and an EM-CCD camera for high-speed single-molecule sensitive imaging. It is also equipped with a heated stage to maintain living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Metasystems Metafer Automated Slide Scanning Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with monochrome and color CCD cameras for the scanning of complete microscope slides with histological or fluorescence stains. The scanning software is also capable of rare cell identification as well as karyotyping of and identification of metaphase spreads.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss StereoLumar V.12 Fluorescence Stereoscope - Biophotonics Core

This stereoscope is equipped with 6 color filtersets for fluorescence excitation as well as a cooled CCD camera for image acquisition. The system also has an white LED illuminator for the transillumination of specimens.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss Libra 120 PLUS Transmission Electron Microscope (120kV voltage system) - Biophotonics Core

This 120kV TEM microscope is equipped with a 2kx2k fiber-optically coupled bottom mount YAG CCD camera for image and montage acquisition, an in-column "Omega" energy filter for high contrast imaging in biological samples and a high-angle tilt holder for tomographic applications.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Leica TCS SP2 AOBS - Confocal Microscope - CCMI Core

This confocal microscope with specially selected high sensitivity color detectors. It is equipped with a programmable beam splitter allowing up to 4 freely variable spectral channels to be acquired simultaneously. Laser lines available are: 405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, 561 nm and 633 nm. The 561 nm DPSS laser provides approximately 10 times greater sensitivity compared with a conventional green HeNe when used with dyes such as Texas Red or Alexa 594. A fast Z drive is present, allowing rapid xz scans.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Molecular Dynamics Typhoon 8600  2D-Scanner - CCMI Core

This variable mode flat bed 2-dimensional scanner may be used to scan phosphor screens to quantitate radioactivity in gels and blots or scan chemifluorescent samples. Its twin lasers (532 nm and 635 nm) enable scanning of fluorescent dyes (including fluorescein-like dyes) in samples.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Perkin-Elmer HTS7000+ - CCMI Core

This plate reader is capable of assessing fluorescence, chemiluminescence or absorbance in standard multi-well plates. A wide range of excitation and emission filters are available. Excitation wavelengths may be chosen in a range between ~260 nm and ~650 nm. The instrument has a built-in incubator and shaker for kinetic studies.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Fuji FLA-5100 - CCMI Core

The Fuji FLA-5100 is a multi-purpose 2-dimensional scanner capable of scanning phosphor screens and fluorescence/chemifluorescence in gels/blots or microplates, and can also digitize existing autorads. The instrument has 4 excitation lasers covering blue to infra-red: (473 nm, 532 nm, 635 nm and 670 nm) allowing it to be used with a wide range of fluors. Maximum scan resolution is 10 µm.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Olympus VivaView FL Incubator-Fluorescence Microscope - Stem Cell Core

This system incorporates an epi-fluorscence inverted microscope built into a bona fide cell culture incubator to permit time-lapse imaging of live cell cultures. It features a fully integrated and motorized inverted microscope with fixed 20x objective for long-term time-lapse imaging by Differential Interference Contrast (DIC) or with fluorescence (red and green). Cells must be grown in special 35mm dishes. Control over temperature, CO2 and oxygen levels are available and there is an access port that allows feeding or compound addition to the cells without fully opening the incubator chamber.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Olympus IX-51 Inverted Fluorescence Microscope - Stem Cell Core

These microscopes (2) are used for routine fluorescence imaging of cell cultures and fixed slides. They are equipped with both monochrome and color cooled CCD cameras. Filter cubes for GFP, RFP, Cy5 and DAPI are available as well as phase-contrast imaging. Principally these microscopes serve users of the STEM Core but access can be made available by prior arrangement.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Becton-Dickinson Special Order LSR II - CCMI Core

This analytical flow cytometer is a 5-laser 15-color instrument. Excitation is provided at 355 nm, 405 nm, 488 nm, 561 nm and 640 nm. In addition to the usual scatter parameters, up to 15 user-defined fluorescence colors may be detected per cell. FACSDiva digital acquisition enables rapid, accurate, sample acquisition.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Becton-Dickinson FACScan - CCMI Core

This is an easy to operate single laser analytical flow cytometer utilizing single line excitation at 488nm. Three fluorescence colors may be detected for each cell (green, orange, red). Single channel pulse processing option is useful for doublet discrimination in cell cycle assays.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Becton-Dickinson FACS Vantage SE DiVa - CCMI Core

This instrument is a high-speed multi-color multi-laser cell sorter. Two scatter parameters and up to 8 fluorescence colors may be collected per cell. Excitation sources include a coherent Sapphire 200 mW 488 nm primary laser, and a Coherent I-300 high-power argon-ion laser which provides primary lines at ~350 nm, 458 nm, 476 nm, 496 nm, 488 nm and 514 nm. Also available are a 35 mW helium-neon laser providing 633 nm excitation and a Coherent Sapphire 200 mW 568 nm unit. TurboSort option allows cell sorting at high speeds, up to 30,000 cells/sec. Large cells may be sorted. Four populations may be sorted simultaneously, or cells may be sorted into multiwell plates.
Location: CCMI Core
Contact: ccmi@salk.edu

 

+ Cyntellect LEAP - Stem Cell Core

This is a unique microplate-based cytometry system for in situ cellular analysis and purification. LEAP combines whole well imaging with laser manipulation for selective purification of in vitro cells in their natural state. Adherent cells can be labeled with fluorescent antibodies and selectively removed using the laser processing. LEAP applications include in situ purification of various cell types, rapid cell line development and stem cell processing and generation.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Affymetrix GeneChip® Scanner 3000 7G - Functional Genomics

This gene-chip scanner processes next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis. Together with the GeneChip Fluidics Station and the GeneChip Hybridization Oven, processing and analysis of a wide variety of affimetrix microarray chips.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ GeneChip® Fluidics Station 450 - Functional Genomics

This instrument is used for the required wash and stain operations of Affymetrix GeneChip® arrays, prior to scanning on the GeneChip Scanner 3000 7G.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ GeneChip® Hybridization Oven 640 - Functional Genomics

This GeneChip hybridization over provides precise temperature and rotation control to ensure the successful hybridization of as many as 64 GeneChip cartridges at one time.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ Applied Biosystems 7900HT - Functional Genomics

This quantitative, real-time PCR system can operate in both 96- and 384-well plate formats. Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction. Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences. This system is equipped with a robotic plate loader for 96-well format allowing 24hr sample processing.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ Qiagen BioRobot 9604 Miniprep Robot - Functional Genomics

This miniprep robot is a molecular biology workstation that enables standardized and automated nucleic acid purifications. It can process samples efficiently in multiples of 8 up to 96 samples. Sample turn-around time is typically within 24 hrs. A bacterial culture shaker-incubator is also available in the Core Facility to grow samples for miniprep of plasmids using this automated system.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ Agilent 2100 Bioanalyzer - Functional Genomics

This microfluidics-based platform is used for sizing, quantification and quality control of DNA, RNA, proteins and cells. The RNA nano kit is available through the FGL Core to check RNA quality before submitting samples for GeneChip analysis. For all other applications, users must provide their own reagents.
Location: Functional Genomics
Contact: Ling Ouyang

 

+ Protein Technologies PS3 Peptide Synthesizer - Peptide Synthesis Core

This synthesizer allows the sequential synthesis of up to three peptides; syntheses can be run at either a 0.1 or 0.5 mmol scale using prepacked cartridges of amino acids purchased by the investigator from Protein Technologies. Hand-packing cartridges allows increased flexibility--other scales of synthesis are possible and the incorporation of non-conventional amino acids is also possible. Synthesis of peptides with specific modifications (phosphorylation, biotinylation, amidation, acetylation) is routine. The crude peptides are characterized by both mass spec and analytical HPLC. When purification of peptides is desired or necessary, Jozsef Gulyas in Jean Rivier's group (PBL) uses an appropriate combination of ion exchange and C18 (or other sizing) HPLC columns. The purified peptides are characterized by another analytical HPLC run and by a capillary electrophoresis run to gauge their final purity.
Location: Peptide Synthesis Core
Contact: Jill Meisenhelder

 

+ Thermo LTQ-Orbitrap XL Electrospray FT-MS - Mass Spectrometry Core

This high resolution mass spectrometer is equipped with a nano-spray source and a nano-flow liquid chromatography system (Eksigent nanoLC-plus). Data Analysis is performed on a UNIX-based Sorcerer Solo and on a PC-based Mascot Server. The instrumentation is used for proteomics analysis of gel separated proteins and for the analysis of complex mixtures in multi-dimensional LC mode (MudPIT).
Location: Mass Spectrometry Core
Contact: Wolfgang Fischer

 

+ Applied Biosystems DE-STR MALDI-TOF-MS - Mass Spectrometry Core

This instrument is used for rapid determination of the intact molecule mass of peptides. Both peptides form natural sources as well as synthetic products are analyzed. The speed of analysis afforded by this instrument allows rapid feed-back during complicated syntheses.
Location: Mass Spectrometry Core
Contact: Wolfgang Fischer

 

+ Olympus FV1000MPE/CARS Two-Photon Laser Scanning Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with the following single-photon laser lines (405, 440, 488, 514, 559, 633nm) as well as a Spectra-Physics MaiTai DeepSee infrared laser for two-photon excitation. It is equipped with 3 internal descanned detectors as well as 4 non-descanned two-photon fluorescence detectors and 2 forward scanning fluorescence detectors for Second Harmonic Generation (SHG) and Coherent Anti-Stokes Raman Microscopy (CARS).
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss LSM 710 Laser Scanning Microscope (inverted platform) - Biophotonics Core

This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal detector for full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss CSU Spinning Disk Live Cell Confocal Microscope (inverted platform) - Biophotonics

This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and a Yokogawa spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Nikon TiE: PFS Live Cell TIRF Microscope (inverted platform) - Biophotonics Core

This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm), an Andor EM-CCD camera and the Nikon patented PerfectFocus module for TIRF imaging. It is also equipped with a stage-top incubation system for maintaining living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss VivaTiome Fluorescence Microscope (inverted platform) - Biophotonics Core

This inverted microscope is an advanced fluorescence microscope equipped with a multi-color LED illumination system and a filtered white light source as well as a high-resolution cooled CCD camera for fluorescence detection. The system is also equipped with a VivaTome unit, which is an aperture correlation based optical sectioning device capable of acquiring confocal images at near video-rate. The system is also equipped with a color CCD camera for visualizing histological samples.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Olympus BX61WI Live Cell Fluorescence Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with a DG-5 fast wavelength switcher for fluorescence excitation and a fast filter wheel for fluorescence emission and an EM-CCD camera for high-speed single-molecule sensitive imaging. It is also equipped with a heated stage to maintain living cells in culture.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Metasystems Metafer Automated Slide Scanning Microscope (upright platform) - Biophotonics Core

This upright microscope is equipped with monochrome and color CCD cameras for the scanning of complete microscope slides with histological or fluorescence stains. The scanning software is also capable of rare cell identification as well as karyotyping of and identification of metaphase spreads.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss StereoLumar V.12 Fluorescence Stereoscope - Biophotonics Core

This stereoscope is equipped with 6 color filtersets for fluorescence excitation as well as a cooled CCD camera for image acquisition. The system also has an white LED illuminator for the transillumination of specimens.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Zeiss Libra 120 PLUS Transmission Electron Microscope (120kV voltage system) - Biophotonics Core

This 120kV TEM microscope is equipped with a 2kx2k fiber-optically coupled bottom mount YAG CCD camera for image and montage acquisition, an in-column "Omega" energy filter for high contrast imaging in biological samples and a high-angle tilt holder for tomographic applications.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Biological Safety Cabinets - Biophotonics Core

Nuaire model NU425-400 HEPA filter vertical laminar flow hoods (2) for tissue culture procedures, and handling cells in an aseptic environment. 1 flow hood is dedicated to virus infected cells.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Cell Culture Incubators - Biophotonics Core

Thermo 3110 incubator chambers (4) are copper lined for antimicrobial protection for the culture of mammalian cells. 1 incubator is dedicated to virus infected cells.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ ThermoFisher Legend X1R Refrigerated Centrifug - Biophotonics Core

This refrigerated benchtop centrifuge with swinging bucket rotor has adaptors for different size tubes. It is often used for the spin down of cells. The rotor has a max rpm rating of 5000.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Olympus CKX41 Phase Contrast Microscope (inverted platform) - Biophotonics Core

Olympus CKX-41 inverted phase contrast microscopes for the observation of living cells in culture plates. Equipped with 5X, 10X and 20X phase contrast objective lenses.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Tissue Culture lab equipment - Biophotonics Core

General support equipment for culturing cells - 37 degree C Water Bath, Microfuge, Vortex Mixer, UltraSonic Water Bath, 4 degree C Refrigerator and -20 degree C Freezer.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ General wet lab equipment - Biophotonics Core

General wet lab equipment for LM and EM sample preparation - Barnsted NanoPure Water System, Metler Toledo High Accuracy Balance, pH Meter, Magnetic Stirplate, Microfuge, Vortex Mixer, Sample Rotator/Rocker, Resin Curing Oven (60 degree C)
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Leica UC7/FC7 Ultracyromicrotome - Biophotonics Core

Ultramicrotome capable of cutting 1 micron thick tissue sections (with glass knives) or 25nm - 500nm tissues sections (with diamond knives). System is also equipped with a cryo unit for frozen section preparation and also Tokuyasu freezing.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Leica EM KMR3 Glass Knife Maker - Biophotonics Core

Glass knife maker for producing edges capable of cutting 1 micron semi-thin tissue sections on the UC7 ultramicrotome
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Leica EM SCD500 Sputter Coater/Evaporator - Biophotonics Core

High vacuum benchtop system for carbon coating TEM grids and sputter coating samples with Gold or Palladium for SEM studies.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ BioWave Pro Tissue Processing Microwave - Biophotonics Core

Microwave based tissue processing system to fix and embed cells or tissue samples in plastic resins for semi-thin and ultra-thin sectioning in the UC7 ultramicrotome.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Chemical Fume Hood - Biophotonics Core

Chemical hood for the fixation and heavy metal staining of cells and tissues for TEM studies.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Olympus SZ61 Zoom Stereoscope - Biophotonics Core

Zoom stereoscope for the manipulation of TEM sample blocks
Location: Biophotonics Core
Contact: James Fitzpatrick

 

+ Olympus BX43 Phase Contrast/Fluorescence Microscope (upright platform) - Biophotonics Core

Phase contrast and fluorescence upright microscope for the investigation and imaging of semi-thin tissue sections prior to ultra-thin sectioning for TEM studies.
Location: Biophotonics Core
Contact: James Fitzpatrick

 

The following equipment is available to Salk researchers for human stem cell research efforts.

+ Cell Culture Incubator Chambers - Stem Cell Core

Thermo 3110 incubator chambers (8) are copper lined for antimicrobial protection. In addition we have an additional (8) NuAire incubator chambers, both types are equipped with CO2 monitoring, HEPA filtered air supply and passive humidity monitoring.
Location: - Stem Cell Core
Contact: Travis Berggren

 

+ Hypoxic (Low Oxygen) Incubator Chambers - Stem Cell Core

Thermo 3130 Copper Lined incubator chambers (2) offer control over the level of O2 (1-20%) and CO2 (1-10%). They are equipped with 8 inner glass doors to minimize the loss of environmental conditions upon access to cell cultures. HEPA filtered air supply and passive humidity monitoring.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Biological Safety Cabinets - Stem Cell Core

(10) total Nuaire model NU425-400 HEPA filter vertical laminar flow hoods are used for tissue culture procedures, and handling cells in an aseptic environment.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Inverted Phase-Contrast Microscopes - Stem Cell Core

(3) Olympus CKX-31 inverted phase-contrast microscopes are used for routine observation of stem cell cultures. (2) Olympus CKX-41 inverted phase contrast microscopes are equipped with a trinocular-head allowing simulataneous observation and imaging via a low resolution CCD camera. Both photography and video recording capabilities are available.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Stereomicroscopes - Stem Cell Core

(3) Olympus SZX-10 stereomicroscopes are available for manual picking of stem cell cultures.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Laminar Flow Hood - Stem Cell Core

A NuAire Laminar Flow Clean Bench provides an increased degree of sterility for open culture stem cell picking operations.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ IX-51 Fluorescence Microscopes - Stem Cell Core

(2) Olympus IX-51 Fluorescence microscopes are used for routine fluorescence imaging of cell cultures and fixed slides. They are equipped with both monochrome and color cooled CCD cameras. Filter cubes for GFP, RFP, Cy5 and DAPI are available as well as phase-contrast imaging.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Beckman Coulter Optima L-80XP Ultracentrifuge - Stem Cell Core

This ultracetrifuge is typically used for the concentration of viral vector preparations. It is equipped with a HEPA filtered chamber – BioSafe option. The SW-32 Ti rotor hold 6x 38ml tubes (max g force 175,000). The SW-55Ti rotor holds 6x 5ml tubes with a max g force of 368,000.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Alegra X-12R Centrifuge - Stem Cell Core

This Benchtop centrifuge with swinging bucket rotor has adaptors for tubes or cell culture plates. It is often used for the spinfection of viral vectors to transduce stem cells. The rotor has a max g force of 3270.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Cell Culture Support Equipment - Stem Cell Core

The facility is equipped with all the requisite support equipment to perform routine stem cell culture including water baths, incubators, refrigeration and freezer storage, clinical centrifuges, vortexers, microcentrifuges, pipets and the like.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ General Lab Support Equipment - Stem Cell Core

Spectrophotometer, MilliQ-water system, analytical balances, pH meter, microcentrifuge, osmometer, CO2 meter, magnetic stir plates.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Cryo Storage - Stem Cell Core

(2) MVE 800 cryo-preservation systems provide reliable storage for frozen stem cell samples in vapor-phase LN2.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Amaxa Nucleofector - Stem Cell Core

This device is used for transfecting a variety of cell types including stem cells with plasmids, RNA or other nucleic acids. Both the single cuvette and 96-well shuttle systems are available. This instrument is on loan from Chemical and Systems Biology Core.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Cyntellect LEAP - Stem Cell Core

This is a unique microplate-based cytometry system for in situ cellular analysis and purification. LEAP combines whole well imaging with laser manipulation for selective purification of in vitro cells in their natural state. Adherent cells can be labeled with fluorescent antibodies and selectively removed using the laser processing. LEAP applications include in situ purification of various cell types, rapid cell line development and stem cell processing and generation.
Location:Stem Cell Core
Contact: Travis Berggren

 

+ Olympus VivaView FL Incubator-Fluorescence Microscope - Stem Cell Core

This system incorporates an epi-fluorscence inverted microscope built into a bona fide cell culture incubator to permit time-lapse imaging of live cell cultures. It features a fully integrated and motorized inverted microscope with fixed 20x objective for long-term time-lapse imaging by Differential Interference Contrast (DIC) or with fluorescence (red and green). Cells must be grown in special 35mm dishes. Control over temperature, CO2 and oxygen levels are available and there is an access port that allows feeding or compound addition to the cells without fully opening the incubator chamber. Location:Stem Cell Core
Contact: Travis Berggren

 

The following equipment is available to the Transgenic Core Staff for use in deriving transgenic or knockout mice for Salk researchers.

+ Inverted Microscope - Transgenic Core

A Nikon Eclipse TE 300 and Diaphot 300 equipped for bright field, phase contrast and Nomarski differential interference contrast. The microscopes have both 35 mm still photography and video capabilities.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Micromanipulators - Transgenic Core

Narshige MO-188 and MN-188 hydraulic micromanipulators are mounted on the Nikon microscopes for the holding and injection of embryos.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ XYCLone Laser - Transgenic Core

Hamilton Thorne Biosciences Laser System ablates and breaches intra-and extra-cellular membranes and is used for microinjections, assisted in-vitro fertilization (IVF) and Intracytoplasmic Sperm Injections (ICSI).
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Vibration-free Tables - Transgenic Core

A gimbal gas piston isolator for veritcal and horizontal isolation of vibration.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Microinjectors - Transgenic Core

PLI-90 pico-injectors guarantee a precise and easily controlled injection process.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Pipette Puller - Transgenic Core

A Sutter Instrument P-97 microprocessor controlled horizontal pipet puller. This pipet puller is used for the production of injection pipets, which can be accurately and repeatedly reproduced.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Microforge - Transgenic Core

Alcatel microscope consists of a heating filament and very finely adjustable holders for heat polishing of transfer pipettes, creating the narrow tips of holding pipettes and the spike on the ES cell injection needles.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Cooling Stages - Transgenic Core

5000 microincubators for ES cell injections to control the temperature on the microscope stage
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Dissection Microscopes and Inverted Microscope - Transgenic Core

A Nikon microscope equipped for brightfield and phase contrast observation.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Biological Safety Cabinet - Transgenic Core

A Nuaire model NU425-400 HEPA filter vertical laminar flow hood is used for tissue culture procedures.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ CO2 Incubators - Transgenic Core

Nuaire and Fisher CO2 incubators which are equipped with infrared CO2 monitoring, HEPA filtered air and humidity monitoring, are used for tissue and embryo culture procedures.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Controlled-rate Freezer - Transgenic Core

A FTS Sysytems Bio Cool III, -80°C freezer is used for embryo cryopreservation.
Location: Transgenic Core
Contact: Yelena Dayn

 

+ Cryo Storage - Transgenic Core

A MVE 600 cryo-preservation system provides reliable storage for frozen embryos and cells in liquid or vapor LN2.
Location: Transgenic Core
Contact: Yelena Dayn

 

The following equipment is available to the Viral Vector Core Staff and for use by Salk researchers for the development, production and application of viral vectors.

+ Cell Culture Incubator Chambers - Viral Vector Core

(2) HeraCell 150i incubator chambers have copper lining and shelving for antimicrobial protection and added biosafety during production of BSL2+ viral vectors. In addition we have (2) Binder C150 incubator chambers. All incubators are equipped with CO2 monitoring, HEPA filtered air supply, passive humidity monitoring and high temperature sterilization cycles.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Biological Safety Cabinets - Viral Vector Core

(3) Nuaire HEPA filtered vertical laminar flow hoods are used for BSL2 and BSL2+ tissue culture procedures, viral vector production and handling cells in an aseptic environment.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Inverted Phase-Contrast Microscope - Viral Vector Core

Olympus Diaphot inverted phase-contrast microscope is used for routine observation of cell cultures and assessment of viral vector producer cells.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Inverted Epi-fluorescence Microscope - Viral Vector Core

Nikon TE2000 inverted phase contrast/fluorescence microscope equipped with dichroic filtersets suitable for UV/Green/Red excitation and imaging, 4x, 10x, 20x and 40x extra long working distance objectives, 100x oil immersion objective, Pixera color CCD camera and a PC loaded with image acquisition software. This system is used for quality control during vector production, as well as functional titration of viral vectors expressing a range of fluorescent reporter proteins.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Cell Culture Support Equipment - Viral Vector Core

The VVC TC facility is equipped with all the necessary support equipment to perform routine cell culture as well as BL2 and BL2+ viral vector production including; water baths, incubators, refrigeration, freezer and ultralow freezer storage, liquid nitrogen storage, aerosol-resistant clinical centrifuges, microcentrifuges, balances, pipets and pipette-aids, nanopure water system etc.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Beckman L-70 Ultracentrifuge with SW27/SW28/SW55ti/70Ti rotors and adapters - Viral Vector Core

This HEPA filtered ultracentrifuge is used intensively for the concentration and purification of multiple viral vector types. The SW27 and SW28 rotors hold 6 x 30ml Konical tubes (max g force - 141,000), the SW55ti holds 6 x 5ml tubes (max g force - 368,000) and the 70Ti holds 8 x 39ml Optiseal tubes (max g force - 504,000).
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Molecular Biology Support Equipment - Viral Vector Core

The VVC molecular biology facility is equipped with all the required equipment to perform standard molecular biology manipulations including; Biorad MJ mini PCR thermal cycler, Eppendorf biophotometer UV/VIS spectrometer, New Brunswick bacterial shaker incubator, UVP Gel-DOC-IT gel doc system, Owl large format agarose gel electrophoresis systems, Bio-Rad high voltage power packs, Bio-Rad protein electrophoresis and western blotting systems, Eppendorf refrigerated benchtop centrifuge, microcentrifuges, shakers, hotplates/stirrers, vortexers, balances, pH meter, water baths, nanopure water system, incubators, fridges, freezers, pipettes, pipette-aids etc.
Location: Viral Vector Core
Contact: Daniel Gibbs

 

+ Tecan Freedom Evo 200 Workstation - Chemical & Systems Biology Core

This automation platform performs general liquid handling and robotic plate manipulation for running various protocols such as chemical synthesis, protein crystallization, cDNA library expression isolation-purification, biochemical and cell-based assay protocols. The system features an 8 channel LiHa and RoMa arms, TeMO 96 head, Te-Shake, Te-Vac, Te-Carousel and 40 plate Liconic incubator. The system can also integrate the Amaxa 96 Shuttle Nucleofector (on loan to STEM). The workstation is available for services, not walk-up operation at this time.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga

 

+ Tecan Safire2 Plate Reader - Chemical & Systems Biology Core

The Safire2 is a multimode reader system capable of reading plates (6-1536 wells) for UV/Vis absorbance, fluorescence (intensity and kinetics) and luminescence. This instrument is available for walk-up use.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga

 

+ Velocity 11 (Agilent) BenchCel System - Chemical & Systems Biology Core

This automation platform features the BenchCel 4R plate stacker integrated with a VSpin tabletop plate centrifuge, PlateLoc plate heat sealer, and VCode plate labeling system. Additionally a Vll PlatePierce module for piercing non-peel seals manually. The flexible operation of this system allows the operators to employ any combination of device operations and sequences for microplate processing.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga

 

+ BioTEK Workcell - Chemical & Systems Biology Core

This automation platform consists of a Caliper Twister II arm, and plate lid cradle, and a BioTEK microFLO Select 1536 dispenser for cell plating (peristaltic) and an ELx405-UCWS microplate washer for cell washing/media exchange with integrated ultrasonic cleaner head. The workcell can be accessed via the Twister II multicomponent programming, or the cell dispenser and plate washer can be used offline in manual mode.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga

 

 

Addition instrumentation and equipment related to Chemical Biology and organic synthesis is available in CBPL for synthesis services or collaborative projects. In general, capabilities exist to produce multigram quantities of compounds (<10 steps) sufficient for in vitro and in vivo testing.
Location:CBPL
Contact: Tom Baiga or or Joe Noel

Reagent Collections

+ Dharmacon human genome siRNA collection - Chemical & Systems Biology Core

A set of 267 96-well plates with 80 smart pools (4 siRNA per well) per plate. Storage in RNAse-free buffer at 2uM, -30C. 80% of the collection is reserved for genome-wide screens, 20% may be access for individual sets (Genome, Druggable, Kinase, GPCR) or large (>200) hit picking sets.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga or Jan Karlseder

 

+ Dharmacon human genome miRNA collection - Chemical & Systems Biology Core

A small set of miMIMICS and miINHIBITORS sufficient for a pilot screen. Collection received at part of the RNAI Global Consortium membership, and is first come/first serve as a complete set. Results must be shared with the Salk Core Committee for evaluation of future purchase.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga or Jan Karlseder

 

+ Open BioSystems/MGC cDNA collection - Chemical & Systems Biology Core

The complete MGC library of human and mouse cDNA. Presently as glycerol stocks, scheduled for expression/isolation/purification in 2011. Once transformed, library sets will be available in CSBC.
Location: MCBL
Contact: Suzy Simon

 

+ HHMI/Salk Small Molecule Library Collection - Chemical & Systems Biology Core

An exploratory set of 960 compounds (scaffold-type) built about 10 pharmacophores, stored at 10uM in 100% DMSO, under inert atmosphere and at -30C. Library copies available in 96 and 384 plates as singletons or pooled per screening against whole organism or primary tissue. Additional analogs (up to 2112) for each pharmacophore available upon collaboration.
Location: Chemical & Systems Biology Core
Contact: Tom Baiga or Joe Noel

 

+ bix.salk.edu - Bioinformatics Core

Bioinformatics cluster, containing blast server and sequence databases, and a wide range of other tools and utilities.
Location: Bioinformatics Core
Contact:

 

+ decypher.salk.edu - Bioinformatics Core

Specialized hardware accelerator for extra-fast domain finding with HMMs and gene prediction in genomic DNA.
Location: Bioinformatics Core
Contact:

 

+ GeneGo - Bioinformatics Core

Commercial database of gene functions and networks.
Location: Bioinformatics Core
Contact: