This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and four color filtersets for both PAL-M and SR-SIM super-resolution imaging. It is equipped with two EM-CCD cameras, an Andor iXon3 897 512×512 35fps camera for PAL-M imaging in TIRF mode and an Andor iXon3 888 1024×1024 9 fps camera for 3D SR-SIM. The microscope also has a dedicated off-line processing workstation for the reconstruction of super-resolution imaging datasets.
This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal GAsP detector for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging.
This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal GAsP detector for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging of living cells.
This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal detector for full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging of living cells.
This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and a Yokogawa spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.
This live cell imaging system is equipped with the following laser lines (405, 488 and 561) and a Yokogawa dual-pinhole spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.
This upright microscope is equipped with the following single-photon laser lines (405, 440, 488, 514, 559, 633nm) as well as a Spectra-Physics MaiTai DeepSee infrared laser for two-photon excitation. It is equipped with 3 internal descanned detectors as well as 4 non-descanned two-photon fluorescence detectors and 2 forward scanning fluorescence detectors for Second Harmonic Generation (SHG) and Coherent Anti-Stokes Raman Microscopy (CARS).
This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm), an Andor EM-CCD camera and the Nikon patented PerfectFocus module for TIRF imaging. It is also equipped with a stage-top incubation system for maintaining living cells in culture.
This virtual slide scanner is equipped with an automated 100 slide loader and multiple imaging modalities. Fluorescent filter cubes include: DAPI, FITC, TRITC, and CY5. The system also takes advantage of an additional emission filter wheel allowing the use of a quad band exciter. In addition, this system can image with Bright field (histology).
This microdissection microscope takes advantage of specialized membrane slides to cut and collect microscopic sections of tissue, or single cells. Capturing the sample in Eppendorf tubes, the user is then able to perform further analysis on their chosen sub-regions or cells.
This inverted fluorescent microscope is equipped with DAPI,FITC, and TRITC filter cubes. The system is also equipped with a vivatome module allowing for confocal-like sectioning of the sample.
The V12 stereo scope is equipped with three different lenses allowing ideal imaging for dissection or large sample fluorescence imaging.
Carl Zeiss Libra 120kV PLUS Energy Filtered Transmission Electron Microscope (TEM) that is equipped with a 2kx2k fiber-optically coupled YAG CCD camera capable of montage imaging at high sensitivity and has an in-column energy filter which yields High-Contrast Imaging, which improves signal to noise ratios in images, as well as Electron Energy Loss Spectroscopy (EELS), which allows the elemental analysis of samples. In addition, the TEM is also equipped with a tilt specimen holder (+/- 75 degrees) for tomographic imaging, an in-column energy filter for electron loss and high-contrast imaging of biological samples, and cryogenically cooled stage for the native imaging of proteins and frozen, hydrated sections. The stage is also designed for single-axis tilting for performing cryo-tomography.
Carl Zeiss SIGMA Variable Pressure Field Emission Gun Scanning Electron Microscope (SEM) that is equipped with secondary electron (SE), backscattered electron (BSE), and variable pressure (VPSE) detectors, STEM detector, and cold-stage. System is also equipped with hardware/software for the ATLAS montage imaging module and the Shuttle and Find correlative microscopy navigation module to generate large tiled images and high-throughput workflow.
The Gatan 3View is a fully automated Serial Block Face Scanning Electron Microscope (SBFSEM) system. An ultramicrotome is mounted inside the vacuum chamber of the SIGMA SEM. The 3View automatically sections and images the exposed block face. The data sets generated can be combined to create z-stacks of a volume and later subsequently manually segmented.
The BPHO core has a dedicated EM sample preparation space within the facility to process samples for electron microscopy. The sample preparation instrumentation includes:
Balance wall, cylinder test, grip strength, gait scan, ladder challenge, open field, rotarod, rotometer, activity monitor, treadmill, hot-cold plate analgesia meter and visual cliff. Von Frey
Barnes maze, startle response, fear condition, Morris water maze, novel object recognition, passive avoidance and Y-maze.
Elevated plus maze, light/dark box, pre-pulse inhibition, social interaction apparatus, spontaneous self-grooming, telemetry system for sleep & seizure monitoring and Y-maze. Porsolt Swim Test
The Becton-Dickinson FACSCanto II™ cytometer is an easy to operate 3-laser (8 colour) analytical flow cytometer.
405nm 30mW -2 detectors (450/50BP, 525/50BP)
488nm 20mW – 4 detectors (530/30BP, 585/42BP, 670LP, 780/60BP)
633nm 17mW – 2 detectors (660/20BP, 780/60BP)
The patented optical design features fiber optics and a gel coupled cuvette flow cell. Fluorescent signals are collected using highly efficient octagon/trigon collection detector arrays that use serial light reflections to guide signals to their target detectors, resulting in highly efficient light collection and providing maximum signal retention at the detector level. This BD serial reflective design further enhances instrument sensitivity by collecting the dimmest emission signals first, moving from the longest wavelengths (typically PE-Cy™7) to the shortest (FITC).to maximize signal detection, increasing sensitivity and resolution. The sample injection tube features a rinse function that automatically washes down the probe between tubes, to reduce sample cross contamination.
The cytometer runs BD FACSDiva acquisition software.
The Becton-Dickinson FACScan is an easy to operate single laser analytical flow cytometer. Excitation is via a 488 nm laser line. In addition to the usual scatter parameters, 3 fluorescence colors may be detected for each cell – fixed filters are green (530/30), orange (585/42), and red (650 LP). The instrument has a pulse-processing option for doublet discrimination.
The Becton-Dickinson LSR II is a 5-laser 15-color analytical flow cytometer. The cytometer runs BD FACSDiva acquisition software.
The Becton-Dickinson FACS Vantage SE DiVa is a high-speed multi-color multi-laser cell sorter.
Excitation Sources
Coherent CUBE 405 solid state laser: 100 mW at 407 nm
Coherent Sapphire 488nm solid state laser: 200 mW at 488 nm
Coherent Sapphire 568nm solid state laser: 200 mW at 568 nm
Becton-Dickinson (BD) DiVa™ digital electronics enable generation of pulse height, area, and width for detected parameters. Regular or large cells may be sorted. DiVa™ electronics allows sorting of up to 4 populations simultaneously, or cells may be sorted into multiwell plates (up to 96w plate format).
BD InFlux™
The Becton-Dickinson Influx™ cytometer is a state-of-the-art, 13 parameter 5-laser high-speed jet-in-air cell sorter.
Excitation Sources
355nm 60mW – 2 detectors (460/50BP, 670/30BP)
405nm 100mW -2 detectors (460/50BP, 520/35BP)
488nm 200mW – 2 detectors (530/40BP, 692/40BP)
561nm 150mW – 3 detectors (593/40BP, 670/30BP, 750LP)
640nm 120mW – 2 detectors (670/30BP, 750LP)
To achieve very sensitive, low noise electronics, the BD Influx system uses analog electronics in the first stage of signal amplification. The output of the analog peak and hold circuitry is digitized using 16-bit ADCs and placed on a patented parallel processing digital bus. The bus handles real-time compensation as well as other digitized signals, including area and width of up to 8 additional pulses.
The Influx has several nozzle sizes available to accommodate a variety of particle sizes and can sort 6 populations simultaneously (for suitable sample types). Cells may be sorted into a variety of tube types or multiwell plates (e.g. 384 well).
Additionally, the BD Influx™ is equipped with an aerosol management option; air is drawn away from the operator through a HEPA filter, reducing the amount of aerosols in the sort chamber for an added level of safety.
The 7900HT Fast Real-Time PCR System is the real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading, and also offers optional Fast real-time PCR capability.
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA.
The Illumina HiSeq 2500 is an ultra high-throughput next-generation sequencer. It uses sequencing-by-synthesis (SBS) chemistry with reversible terminators and a highly efficient DNA polymerase modified to accept reversible terminator nucleotides, labeled with four different fluorescent dyes. A time delayed integration (TDI) scanner and four CCD sensors take high-resolution images. Fluorescent dyes on the nucleotides are then cleaved after imaging, and reversible terminators are removed to allow chain extension for next cycle. The HiSeq optics can perform dual surface imaging to process both top and bottom of the flow cell enabling the generation of up to 500 gigabases (Gb) per run and 167 Gb per day. The HiSeq sequencing system can be operated in single or dual flow cell mode to allow flexibility in accommodating different users’ needs for different read lengths and run parameters.
The Illumina cBot system provides completely automated clonal amplification of billions of single molecule DNA sequencing libraries producing clusters, which are randomly distributed on the Illumina HiSeq Flowcell in less than 3 hours. Flow cells are ready to be sequenced after clustering on the cBot system.
The Illumina NextSeq 500 Sequencer uses the same proven reversible-terminator SBS chemistry without the need to collect enough samples to start a sequencing run. This sequencer is particularly designed for time-sensitive and pilot experiments. NextSeq 500 can produce up to 100Gb per day with 400 million reads passing filter.
The NeoPrep System is a digital microfluidic system, which can reproducibly prepare high-quality DNA and RNA sequencing libraries using low amounts of nucleic acid as input, with minimal hands-on time and complete walk-away during the process. Using this system, a single operator can prepare sequencing libraries up to 16 samples in less than 10 hours, thus significantly reducing hands-on time, variability between experiments and potential cross-contamination from precious samples.
The Agilent 2200 TapeStation is a comprehensive quality control instrument for nucleic acids. The TapeStation automates RNA and DNA loading, separation and imaging. Each run can process up to 16 samples in as little as 2 minutes per sample. The TapeStation is our most sensitive instrument for detecting DNA as little as 10pg/uL or 100pg/uL for RNA. The TapeStation can detect nucleic acids from 50bp up to 15kB and only requires 1uL of sample.
The Covaris M220 Sonicator provides reproducible and precise fragmentation of nucleic acids and chromatin. It can transmit acoustic energy at ultrasonic frequencies isothermally without contacts with probes. Preloaded Covaris programs can generate fragment sizes between 150bp and 5000bp, in 15uL up to 1ml of volume.
The QX200 ddPCR system provides an absolute measure of target DNA molecules with high sensitivity using Taqman probe-based or Evagreen assays. The QX200 ddPCR system can be applied to determining copy number variations (CNV), mutation, gene expression analysis, enrichment of rare targeted sequences and CRISPR/Cas9 clones screenings. Each run can process up to 96 samples with each reaction partitioned into 20,000 or more emulsion droplets, totaling ~1.5 millions droplets per plate.
The Qubit Fluorometer can detect DNA, RNA and protein using Qubit quantitation assays. The concentration of the is determined by the association of fluorometric dye and target molecules, which eliminates overestimation in the presence of contaminants.
The TECAN M200 plate reader is equipped with tunable fluorescence excitation and emission with a range of 230nm-850nm and luminescence. Samples can be heated from room temperature to 42C, or mixed by orbital shaking. The system is also equipped with Magellan software for programmable detection and automated analysis.
The EpiShear is a programmable ultrasonic processor (120W), which can shear chromatin or DNA from 500uL to 15ml. Chromatin shearing for Chromatin Immunoprecipitation (ChIP) has been shown to be reproducible and robust. The buildup of heat in temperature-sensitive samples can be prevented by pulse-mode and cold blocks.
This instrument is capable of synthesizing two peptides at once, at scales of 100, 300 or 500 uM. The real time UV monitoring system provides feedback so that deprotection and coupling times are automatically lengthened for problematic sequence, to ensure each cycle is complete.
The Thermo Orbitrap Fusion mass spectrometer combines its three mass analyzers -quadrupole, Orbitrap and linear ion trap in a Tribrid architecture that provides unprecedented depth of analysis for complex biological samples. It can perform a wide variety of analyses, from characterization of complex PTMs as well as comprehensive qualitative and quantitative analysis. Multiple fragmentation techniques (CID, HCD, and ETD) with fragment ion detection in either the ion trap or Orbitrap mass analyzer are available.
The Thermo Q-Exactive mass spectrometer combines quadrupole precursor selection with high-resolution, accurate-mass Orbitrap detection. It generates high quality MS and MS/MS data. Its advanced quadrupole technology improves quantitation of low-abundance ions in complex matrices.
The TSQ Quantiva triple quadrupole mass spectrometer employs Active Ion Management (AIM) to optimize ion creation and transmission from the source to the detector, resulting in extreme sensitivity. This enhanced level of sensitivity greatly improves results in applications such as high-throughput targeted MS experiments and targeted quantitation of small molecules.
This hybrid instrument consists of a linear ion trap and an electrostatic Fourier transform analyzer and is equipped with a nano-spray source. The instrument affords high sensitivity, in the low femtomole range for peptides, and mass accuracies in the single ppm range. The instrument is used for proteomic analysis of proteins either separated by gel electrophoresis or by two-dimensional chromatography (MudPIT). Precursor masses are determined in the high resolution Orbitrap with <10ppm accuracy. Fragmentation is achieved by collisional or higher energy dissociation (CID or HCD). In addition to proteomic analyses, the instrument is capable of analyzing small intact proteins to obtain accurate masses.
This imaging system resides within a dedicated CO2 cell culture incubator for extended live cell imaging experiments. It is capable of phase contrast plus red/green fluorescence detection. This system has 4x, 10x and 20x objectives, and can accept a wide variety of standard cell culture dishes and multi-well plates.
Image live or fixed cells in 6-384 well format with bright field and/or RGB fluorescence
(Olympus) with a wide range of filter cubes for common fluorophores. The core has two of these microscopes, both with monochrome ccd cameras, one also equipped with color camera.
These “picking-scopes” are used for iPSC colony derivation and manipulation of stem cell cultures.
A Nikon Eclipse TE 300 and Diaphot 300 equipped for bright field, phase contrast and Nomarski differential interference contrast. The microscopes have both 35 mm still photography and video capabilities.
Narshige MO-188 and MN-188 hydraulic micromanipulators are mounted on the Nikon microscopes for the holding and injection of embryos.
Hamilton Thorne Biosciences Laser System ablates and breaches intra-and extra-cellular membranes and is used for microinjections, assisted in-vitro fertilization (IVF) and Intracytoplasmic Sperm Injections (ICSI).
A gimbal gas piston isolator for veritcal and horizontal isolation of vibration.
PLI-90 pico-injectors guarantee a precise and easily controlled injection process.
A Sutter Instrument P-97 microprocessor controlled horizontal pipet puller. This pipet puller is used for the production of injection pipets, which can be accurately and repeatedly reproduced.
Alcatel microscope consists of a heating filament and very finely adjustable holders for heat polishing of transfer pipettes, creating the narrow tips of holding pipettes and the spike on the ES cell injection needles.
5000 microincubators for ES cell injections to control the temperature on the microscope stage.
A Nikon microscope equipped for brightfield and phase contrast observation.
A Nuaire model NU425-400 HEPA filter vertical laminar flow hood is used for tissue culture procedures.
Nuaire and Fisher CO2 incubators which are equipped with infrared CO2 monitoring, HEPA filtered air and humidity monitoring, are used for tissue and embryo culture procedures.
Controlled-rate Freezer
A FTS Sysytems Bio Cool III, -80°C freezer is used for embryo cryopreservation.
A MVE 600 cryo-preservation system provides reliable storage for frozen embryos and cells in liquid or vapor LN2.
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