Wolfgang Busch

Associate Professor

Plant Molecular and Cellular Biology Laboratory

Integrative Biology Laboratory

Salk Institute for Biological Studies - Publications

List of Publications

Bouain N, Satbhai SB, Korte A, Saenchai C, Desbrosses G, Berthomieu P, Busch W#, Rouached H#. Natural allelic variation of the AZI1 gene controls root growth under zinc-limiting condition. PLoS Genet. 2018;14(4):e1007304. Epub 2018/04/03. doi: 10.1371/journal.pgen.1007304. PubMed PMID: 29608565. [#co-corresponding author]

Shibata M, Breuer C, Kawamura A, Clark NM, Rymen B, Braidwood L, Morohashi K, Busch W, Benfey PN, Sozzani R, Sugimoto K. GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis. Development. 2018;145(3). Epub 2018/02/14. doi: 10.1242/dev.159707. PubMed PMID: 29439132.

Kisko M, Bouain N, Safi A, Medici A, Akkers RC, Secco D, Fouret G, Krouk G, Aarts MG, Busch W, Rouached H. LPCAT1 controls phosphate homeostasis in a zinc-dependent manner. eLife. 2018;7. Epub 2018/02/18. doi: 10.7554/eLife.32077. PubMed PMID: 29453864.

Exposito-Alonso M, Becker C, Schuenemann VJ, Reiter E, Setzer C, Slovak R, Brachi B, Hagmann J, Grimm DG, Chen J, Busch W, Bergelson J, Ness RW, Krause J, Burbano HA, Weigel D. The rate and potential relevance of new mutations in a colonizing plant lineage. PLoS Genet. 2018;14(2):e1007155. Epub 2018/02/13. doi: 10.1371/journal.pgen.1007155. PubMed PMID: 29432421.

Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, Layeghifard M, Neuhold J, Lehner A, Kong J, Grünwald K, Weinberger N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir Y. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018. doi: 10.1038/nature25184

Munch D, Gupta V, Mun T, Bachmann A, Busch W, Kelly S, Andersen SU (2017). The Brassicaceae family displays divergent, shoot-skewed NLR resistance gene expression. Plant Physiol. doi: 10.1104/pp.17.01606. PubMed PMID: 29187571.

Di Mambro R, De Ruvo M, Pacifici E, Salvi E, Sozzani R, Benfey PN, Busch W, Novak O, Ljung K, Di Paola L, Maree AFM, Costantino P, Grieneisen VA, Sabatini S.(2017) Auxin minimum triggers the developmental switch from cell division to cell differentiation in the Arabidopsis root. Proc Natl Acad Sci U S A.;114(36):E7641-E9. doi: 10.1073/pnas.1705833114. PubMed PMID: 28831001; PMCID: PMC5594665.

Barbez E, Dünser K, Gaidora A, Lendl T, Busch W (2017) Auxin steers root cell expansion via apoplastic pH regulation in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2017 May 30. pii: 201613499. doi: 10.1073/pnas.1613499114.

Satbhai SB, Setzer C, Freynschlag F, Slovak R, Kerdaffrec E, Busch W (2017) Natural allelic variation of FRO2 modulates Arabidopsis root growth under iron deficiency. Nat Commun. 2017 May 24;8:15603. doi: 10.1038/ncomms15603.

Vad V, Cedrim D, Busch W, Filzmoser P, Viola I (2017) Generalized box-plot for root growth ensembles. BMC Bioinformatics. 2017 Feb 15;18(Suppl 2):65. doi: 10.1186/s12859-016-1445-3.

Klasen JR, Barbez E, Meier L, Meinshausen N, Bühlmann P, Koornneef M, Busch W, Schneeberger K (2016) A multi-marker association method for genome-wide association studies without the need for population structure correction. Nat Commun 10;7:13299. doi: 10.1038/ncomms13299.

Ristova D, Carré C, Pervent M, Medici A, Kim GJ, Scalia D, Ruffel S, Birnbaum KD, Lacombe B, Busch W, Coruzzi GM, Krouk G (2016) Combinatorial interaction network of transcriptomic and phenotypic responses to nitrogen and hormones in the Arabidopsis thaliana root.Sci Signal 25;9(451):rs13.

Janusch I, Kropatsch WG, Busch W (2014) Reeb graph based examination of root development. Proceedings of the 19th Computer Vision Winter Workshop: 43-50.

Janusch I, Kropatsch WG, Busch W (2014) Topological image analysis and (normalised) representations for plant phenotyping. 16th Internation Syposium on Symbolic and Numeric Algorithms for Scientific Computing: 579-586.

Slovak R, Goschl C, Su X, Shimotani K, Shiina T, Busch W (2014) A Scalable Open- Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis. Plant Cell 26: 2390-2403

Schuster C, Gaillochet C, Medzihradszky A, Busch W, Daum G, Krebs M, Kehle A, Lohmann JU (2014) A Regulatory Framework for Shoot Stem Cell Control Integrating Metabolic, Transcriptional, and Phytohormone Signals. Dev Cell 28: 438-449

Meijon M, Satbhai SB, Tsuchimatsu T, Busch W (2014) Genome-Wide Association Study Using Cellular Traits Identifies a New Regulator of Root Development in Arabidopsis. Nature Genetics 46: 77-81

Ischebeck T, Werner S, Krishnamoorthy P, Lerche J, Meijon M, Stenzel I, Lofke C, Wiessner T, Im YJ, Perera IY, Iven T, Feussner I, Busch W, Boss WF, Teichmann T, Hause B, Persson S, Heilmann I (2013) Phosphatidylinositol 4,5-Bisphosphate Influences PIN Polarization by Controlling Clathrin-Mediated Membrane Trafficking in Arabidopsis. Plant Cell 25: 4894-4911

Busch W, Moore BT, Martsberger B, Mace DL, Twigg RW, Jung J, Pruteanu-Malinici I, Kennedy SJ, Fricke GK, Clark RL, Ohler U, Benfey PN (2012) A Microfluidic Device and Computational Platform for High-Throughput Live Imaging of Gene Expression. Nature Methods 9: 1101-1106

Iyer-Pascuzzi AS, Jackson T, Cui H, Petricka JJ, Busch W, Tsukagoshi H, Benfey PN (2011) Cell Identity Regulators Link Development and Stress Responses in the Arabidopsis Root. Developmental Cell 21: 770-782

Tsukagoshi H, Busch W, Benfey PN (2010) Transcriptional Regulation of ROS Controls Transition from Proliferation to Differentiation in the Root. Cell 143: 606-616

Sozzani R, Cui H, Moreno-Risueno MA, Busch W, Van Norman JM, Vernoux T, Brady SM, Dewitte W, Murray JA, Benfey PN (2010) Spatiotemporal Regulation of Cell-Cycle Genes by SHORTROOT Links Patterning and Growth. Nature 466: 128- 132

Long TA, Tsukagoshi H, Busch W, Lahner B, Salt DE, Benfey PN (2010) The bHLH Transcription Factor POPEYE Regulates Response to Iron Deficiency in Arabidopsis Roots. Plant Cell 22: 2219-2236

Busch W, Miotk A, Ariel FD, Zhao Z, Forner J, Daum G, Suzaki T, Schuster C, Schultheiss SJ, Leibfried A, Haubeiss S, Ha N, Chan RL, Lohmann JU (2010) Transcriptional Control of a Plant Stem Cell Niche. Developmental Cell 18: 849-861

Schultheiss SJ, Busch W, Lohmann JU, Kohlbacher O, Ratsch G (2009) KIRMES: Kernel-Based Identification of Regulatory Modules in Euchromatic Sequences. Bioinformatics 25: 2126-2133

Kumar M, Busch W, Birke H, Kemmerling B, Nurnberger T, Schoffl F (2009) Heat Shock Factors HsfB1 and HsfB2b are Involved in the Regulation of Pdf1.2 Expression and Pathogen Resistance in Arabidopsis. Molecular Plant 2: 152-165

Fulton L, Batoux M, Vaddepalli P, Yadav RK, Busch W, Andersen SU, Jeong S, Lohmann JU, Schneitz K (2009) DETORQUEO, QUIRKY, and ZERZAUST Represent Novel Components Involved in Organ Development Mediated by the Receptor-lLike Kinase STRUBBELIG in Arabidopsis thaliana. PLoS Genet 5: e1000355

Skirycz A, Radziejwoski A, Busch W, Hannah MA, Czeszejko J, Kwasniewski M, Zanor MI, Lohmann JU, De Veylder L, Witt I, Mueller-Roeber B (2008) The DOF Transcription Factor OBP1 is Involved in Cell Cycle Regulation in Arabidopsis thaliana. Plant J 56: 779-792

Laubinger S, Sachsenberg T, Zeller G, Busch W, Lohmann JU, Ratsch G, Weigel D (2008) Dual Roles of the Nuclear Cap-Binding Complex and SERRATE in pre-mRNA Splicing and microRNA Processing in Arabidopsis thaliana. Proc Natl Acad Sci U S A 105: 8795-8800

Andersen SU, Buechel S, Zhao Z, Ljung K, Novak O, Busch W, Schuster C, Lohmann JU (2008) Requirement of B2-type Cyclin-Dependent Kinases for Meristem Integrity in Arabidopsis thaliana. Plant Cell 20: 88-100

Levesque MP, Vernoux T, Busch W, Cui H, Wang JY, Blilou I, Hassan H, Nakajima K, Matsumoto N, Lohmann JU, Scheres B, Benfey PN (2006) Whole-Genome Analysis of the SHORT-ROOT Developmental Pathway in Arabidopsis. PLoS Biology 4: e143

Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohmann JU, Weigel D (2005) Integration of Spatial and Temporal Information During Floral Induction in Arabidopsis. Science 309: 1056-1059

Leibfried A, To JP, Busch W, Stehling S, Kehle A, Demar M, Kieber JJ, Lohmann JU (2005) WUSCHEL Controls Meristem Function by Direct Regulation of Cytokinin- Inducible Response Regulators. Nature 438: 1172-1175

Busch W, Wunderlich M, Schoffl F (2005) Identification of Novel Heat Shock Factor- Dependent Genes and Biochemical Pathways in Arabidopsis thaliana. Plant J 41: 1- 14

Pivetti CD, Yen MR, Miller S, Busch W, Tseng YH, Booth IR, Saier MH, Jr. (2003) Two Families of Mechanosensitive Channel Proteins. Microbiol Mol Biol Rev 67: 66- 85, table of contents

Invited Reviews

Ogura T, Busch W (2016) Genotypes, Networks, Phenotypes: Moving Toward Plant Systems Genetics.
Annu Rev Cell Dev Biol. 6;32:103-126.

Smakowska E, Kong J, Busch W, Belkhadir Y (2016) Organ-specific regulation of growth-defense tradeoffs by plants. Curr Opin Plant Biol.29:129-37. doi: 10.1016/j.pbi.2015.12.005. Review.

Slovak R, Ogura T, Satbhai SB, Ristova D, Busch W (2016) Genetic control of root growth: from genes to networks.
Ann Bot. 117(1):9-24. doi: 10.1093/aob/mcv160. Review.

Satbhai SB, Ristova D, Busch W (2015) Underground tuning: quantitative regulation of root growth. J Exp Bot 66(4):1099-1112.

Ogura T and Busch W. From phenotypes to causal sequences: The use of genome wide association studies to dissect the sequence basis for variation of plant development. Current Opinion in Plant Biology 23C: 98-108

Ristova D, Busch W (2014) Natural variation of Root Traits: from Development to Nutrient Uptake. Plant Physiol 166: 518-527

Sozzani R, Busch W, Spalding EP, Benfey PN (2014) Advanced Imaging Techniques for the Study of Plant Growth and Development. Trends Plant Sci 19: 304-310

Moreno-Risueno MA, Busch W, Benfey PN (2010) Omics Meet Networks - Using Systems Approaches to Infer Regulatory Networks in Plants. Current Opinion in Plant Biology 13: 126-131

Busch W, Benfey PN (2010) Information Processing Without Brains--the Power of Intercellular Regulators in Plants. Development 137: 1215-1226

Busch W, Lohmann JU (2007) Profiling a Plant: Expression Analysis in Arabidopsis. Curr Opin Plant Biol 10: 136-141


Diploma, Biology, University of Tübingen, Germany 2004
PhD, Biology, University of Tübingen, Germany 2009

Awards & Honors

  • 2015 Society for Experimental Biology President's Medal 2015
  • 2014 Annals of Botany Lecture; ICAR 2014; Vancouver; Canada

  • 2013 Genome Web Young Investigator, GenomeWeb Intelligence Network, USA
  • 2009 Doctoral thesis award, Reinhold-und-Maria-T eufel Foundation, Germany
  • 2008 PhD summa cum laude, University of Tübingen, Germany
  • 2004 PhD - Fellowship of the Cusanuswerk, Germany

Science Guide