Gene Transfer, Targeting and Therapeutics
Viral Vector Core

Salk Institute NINDS-R24 Resource Page for:
Neural Circuit Structure and Function Studies using G-Deleted Rabies Viruses

PI: Edward Callaway

SUMMARY:
Understanding how neurological diseases impair brain function (e.g., memory retrieval and the control of movement) requires a detailed understanding of the affected cell types and neural circuits. A modified version of the rabies virus can be used to trace and experimentally manipulate neural circuits, but these cutting-edge reagents are difficult to produce and are not readily available to the neuroscience community. To generate these reagents in a more efficient manner and to encourage the use of this technology by new research groups, we have established a facility to maintain, propagate, distribute, and improve the G-deleted rabies virus system.

FACILITIES:
This NINDS funded resource is supported by the Salk Institute Core Facility for Gene Transfer, Targeting and Therapeutics (GT3 Core). The Core consists of a large 500-ft2 tissue culture (TC) room and a 500-ft2 main laboratory area, which is equipped to meet the requirements of currently offered services. These services include cloning and production of G-deleted rabies vectors, lentiviral vectors, adenoviral vectors, AAV, and VSV vectors. The TC room is equipped with 5 biological safety cabinets (2 designated for BSL2 and 3 for BSL2+ work), 5 TC incubators, an LED fluorescent microscope with camera, ultracentrifuges, and the full compliment of molecular biology and analytical equipment to meet a wide array of viral vector production needs.

PRODUCTS:
The following is a listing of commonly used stock reagents available through this resource.

Lentiviral Vectors

Rabies Viral Vectors

Production of custom: G-deleted rabies, rAAV, as well as lentiviral, retroviral, and adenoviral vectors specific for individual NINDS investigator needs.

ORDERING:
To order specific products and information on rates, please follow the link below:
https://www.salk.edu/science/core-facilities/viral-vector-core/scheduling-and-rates/

VIRTUAL KITS:
https://www.salk.edu/science/core-facilities/viral-vector-core/ninds-r24-resource-page/virtual-kits/

ACKNOWLEDGING SUPPORT:
Research reported was supported by the National Institute Of Neurological Disorders And Stroke of the National Institutes of Health under Award Number R24NS092943. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.