Gerald Shadel

Professor

Molecular and Cell Biology Laboratory

Audrey Geisel Chair in Biomedical Science

Salk Institute for Biological Studies - Publications

all Publications


2019

Harel, M., Ortenberg, R., Varanasi, S.K., Mangalhara, K.C., Mardamshina, M., Markovits, E., Baruch, E.N., Tripple, V., Arama-Chayoth, M., Greenberg, E., Shenoy, A., Ayasun, R., Knafo, N., Xu, S., Anafi, L., Yanovich-Arad, G., Barnabas, G.D., Ashkenazi, S., Besser, M.J., Schachter, J., Bosenberg, M., Shadel, G.S., Barshack, I., Kaech, S.M., Markel, G., Geiger, T. Proteomics of Melanoma Response to Immunotherapy Reveals Mitochondrial Dependence. (2019) Cell. DOI: 10.1016/j.cell.2019.08.012

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2018

Mangalhara, K.C., Shadel, G.S. A Mitochondrial-Derived Peptide Exercises the Nuclear Option. (2018) Cell Metabolism. 28(3):330-331. DOI: 10.1016/j.cmet.2018.08.017

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Newman, L.E., Shadel, G.S. Pink1/Parkin link inflammation, mitochondrial stress, and neurodegeneration. (2018) Journal of Cell Biology. 217(10):3327-3329. DOI: 10.1083/jcb.201808118

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Cox, C.S., McKay, S.E., Holmbeck, M.A., Christian, B.E., Scortea, A.C., Tsay, A.J., Newman, L.E., Shadel, G.S. Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling. (2018) Cell Metabolism. 28(5):776-786. DOI: 10.1016/j.cmet.2018.07.011

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Wu, L., Cao, J., Cai, W.L., Lang, S.M., Horton, J.R., Jansen, D.J., Liu, Z.Z., Chen, J.F., Zhang, M., Mott, B.T., Pohida, K., Rai, G., Kales, S.C., Henderson, M.J., Hu, X., Jadhav, A., Maloney, D.J., Simeonov, A., Zhu, S., Iwasaki, A., Hall, M.D., Cheng, X., Shadel, G.S., Yan, Q. KDM5 histone demethylases repress immune response via suppression of STING. (2018) PLOS Biology. 16(8):e2006134. DOI: 10.1371/journal.pbio.2006134

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Zhang, Y., Lee, J.H., Paull, T.T., Gehrke, S., D'Alessandro, A., Dou, Q., Gladyshev, V.N., Schroeder, E.A., Steyl, S.K., Christian, B.E., Shadel, G.S. Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity. (2018) Science Signaling. 11(538). DOI: 10.1126/scisignal.aaq0702

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Kuszak, A.J., Espey, M.G., Falk, M.J., Holmbeck, M.A., Manfredi, G., Shadel, G.S., Vernon, H.J., Zolkipli-Cunningham, Z. Nutritional Interventions for Mitochondrial OXPHOS Deficiencies: Mechanisms and Model Systems. (2018) Annual Review of Pathology. 13(163-191):163-191. DOI: 10.1146/annurev-pathol-020117-043644

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2017

West, A.P., Shadel, G.S. Mitochondrial DNA in innate immune responses and inflammatory pathology. (2017) Nature Reviews. Immunology. 17(6):363-375. DOI: 10.1038/nri.2017.21

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Schweppe, D.K., Chavez, J.D., Lee, C.F., Caudal, A., Kruse, S.E., Stuppard, R., Marcinek, D.J., Shadel, G.S., Tian, R., Bruce, J.E. Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry. (2017) Proceedings of the National Academy of Sciences of the United States of America. 114(7):1732-1737. DOI: 10.1073/pnas.1617220114

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2016

Oran, A.R., Adams, C.M., Zhang, X.Y., Gennaro, V.J., Pfeiffer, H.K., Mellert, H.S., Seidel, H.E., Mascioli, K., Kaplan, J., Gaballa, M.R., Shen, C., Rigoutsos, I., King, M.P., Cotney, J.L., Arnold, J.J., Sharma, S.D., Martinez-Outschoorn, U.E., Vakoc, C.R., Chodosh, L.A., Thompson, J.E., Bradner, J.E., Cameron, C.E., Shadel, G.S., Eischen, C.M., McMahon, S.B. Multi-focal control of mitochondrial gene expression by oncogenic MYC provides potential therapeutic targets in cancer. (2016) Oncotarget. 7(45):72395-72414. DOI: 10.18632/oncotarget.11718

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Kang, M.J., Shadel, G.S. A Mitochondrial Perspective of Chronic Obstructive Pulmonary Disease Pathogenesis. (2016) Tuberculosis and Respiratory Diseases. 79(4):207-213. DOI: 10.4046/trd.2016.79.4.207

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Holmbeck, M.A., Shadel, G.S. Mitochondria provide a 'complex' solution to a bacterial problem. (2016) Nature Immunology. 17(9):1009-10. DOI: 10.1038/ni.3534


Nouws, J., Goswami, A.V., Bestwick, M., McCann, B.J., Surovtseva, Y.V., Shadel, G.S. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. (2016) Journal of Biological Chemistry. 291(2):989-97. DOI: 10.1074/jbc.M115.689299

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2015

McKay, S.E., Yan, W., Nouws, J., Thormann, M.J., Raimundo, N., Khan, A., Santos-Sacchi, J., Song, L., Shadel, G.S. Auditory Pathology in a Transgenic mtTFB1 Mouse Model of Mitochondrial Deafness. (2015) Am. J. Pathol. 185(12):3132-40. DOI: 10.1016/j.ajpath.2015.08.014

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Camell, C.D., Nguyen, K.Y., Jurczak, M.J., Christian, B.E., Shulman, G.I., Shadel, G.S., Dixit, V.D. Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity. (2015) Journal of Biological Chemistry. 290(49):29402-13. DOI: 10.1074/jbc.M115.680199

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Shadel, G.S., Horvath, T.L. Mitochondrial ROS signaling in organismal homeostasis. (2015) Cell. 163(3):560-9. DOI: 10.1016/j.cell.2015.10.001

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Longo, V.D., Antebi, A., Bartke, A., Barzilai, N., Brown-Borg, H.M., Caruso, C., Curiel, T.J., de Cabo, R., Franceschi, C., Gems, D., Ingram, D.K., Johnson, T.E., Kennedy, B.K., Kenyon, C., Klein, S., Kopchick, J.J., Lepperdinger, G., Madeo, F., Mirisola, M.G., Mitchell, J.R., Passarino, G., Rudolph, K.L., Sedivy, J.M., Shadel, G.S., Sinclair, D.A., Spindler, S.R., Suh, Y., Vijg, J., Vinciguerra, M., Fontana, L. Interventions to Slow Aging in Humans: Are We Ready? (2015) Aging Cell. 14(4):497-510. DOI: 10.1111/acel.12338

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Kang, M.J., Yoon, C.M., Kim, B.H., Lee, C.M., Zhou, Y., Sauler, M., Homer, R., Dhamija, A., Boffa, D., West, A.P., Shadel, G.S., Ting, J.P., Tedrow, J.R., Kaminski, N., Kim, W.J., Lee, C.G., Oh, Y.M., Elias, J.A. Suppression of NLRX1 in chronic obstructive pulmonary disease. (2015) Journal of Clinical Investigation. 125(6):2458-62. DOI: 10.1172/JCI71747

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2014

Rongvaux, A., Jackson, R., Harman, C.C., Li, T., West, A.P., de Zoete, M.R., Wu, Y., Yordy, B., Lakhani, S.A., Kuan, C.Y., Taniguchi, T., Shadel, G.S., Chen, Z.J., Iwasaki, A., Flavell, R.A. Apoptotic caspases prevent the induction of type I interferons by mitochondrial DNA. (2014) Cell. 159(7):1563-77. DOI: 10.1016/j.cell.2014.11.037

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Clayton, D.A., Shadel, G.S. Isolation of mitochondria from cells and tissues. (2014) Cold Spring Harbor Protocols. 2014(10):pdb.top074542. DOI: 10.1101/pdb.top074542

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Clayton, D.A., Shadel, G.S. Purification of mitochondria by sucrose step density gradient centrifugation. (2014) Cold Spring Harbor Protocols. 2014(10):pdb.prot080028. DOI: 10.1101/pdb.prot080028

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Clayton, D.A., Shadel, G.S. Isolation of mitochondria from animal tissue. (2014) Cold Spring Harbor Protocols. 2014(10):pdb.prot080010. DOI: 10.1101/pdb.prot080010

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Clayton, D.A., Shadel, G.S. Isolation of mitochondria from tissue culture cells. (2014) Cold Spring Harbor Protocols. 2014(10):pdb.prot080002. DOI: 10.1101/pdb.prot080002

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Nouws, J., Shadel, G.S. microManaging mitochondrial translation. (2014) Cell. 158(3):477-8. DOI: 10.1016/j.cell.2014.07.010

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Shadel, G.S. Live longer on MARS: a yeast paradigm of mitochondrial adaptive ROS signaling in aging. (2014) Microbial Cell. 1(5):140-144. DOI: 10.15698/mic2014.05.143

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Mannam, P., Shinn, A.S., Srivastava, A., Neamu, R.F., Walker, W.E., Bohanon, M., Merkel, J., Kang, M.J., Dela Cruz, C.S., Ahasic, A.M., Pisani, M.A., Trentalange, M., West, A.P., Shadel, G.S., Elias, J.A., Lee, P.J. MKK3 regulates mitochondrial biogenesis and mitophagy in sepsis-induced lung injury. (2014) Am. J. Physiol. Lung Cell Mol. Physiol. 306(7):L604-19. DOI: 10.1152/ajplung.00272.2013

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Christian, B.E., Shadel, G.S. Aging: it's SIRTainly possible to restore mitochondrial dysfunction. (2014) Current Biology. 24(5):R206-8. DOI: 10.1016/j.cub.2014.01.027

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Schroeder, E.A., Shadel, G.S. Crosstalk between mitochondrial stress signals regulates yeast chronological lifespan. (2014) Mech. Ageing Dev. 135:41-9. DOI: 10.1016/j.mad.2013.12.002

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Sharma, N.K., Lebedeva, M., Thomas, T., Kovalenko, O.A., Stumpf, J.D., Shadel, G.S., Santos, J.H. Intrinsic mitochondrial DNA repair defects in Ataxia Telangiectasia. (2014) DNA Repair. 13:22-31. DOI: 10.1016/j.dnarep.2013.11.002

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2013

Schroeder, E.A., Raimundo, N., Shadel, G.S. Epigenetic silencing mediates mitochondria stress-induced longevity. (2013) Cell Metabolism. 17(6):954-64. DOI: 10.1016/j.cmet.2013.04.003

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Bestwick, M.L., Shadel, G.S. Accessorizing the human mitochondrial transcription machinery. (2013) Trends in Biochemical Sciences. 38(6):283-91. DOI: 10.1016/j.tibs.2013.03.006

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Surovtseva, Y.V., Shadel, G.S. Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. (2013) Nucleic Acids Research. 41(4):2479-88. DOI: 10.1093/nar/gks1447

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2012

Ocampo, A., Liu, J., Schroeder, E.A., Shadel, G.S., Barrientos, A. Mitochondrial respiratory thresholds regulate yeast chronological life span and its extension by caloric restriction. (2012) Cell Metabolism. 16(1):55-67. DOI: 10.1016/j.cmet.2012.05.013

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Longo, V.D., Shadel, G.S., Kaeberlein, M., Kennedy, B. Replicative and chronological aging in Saccharomyces cerevisiae. (2012) Cell Metabolism. 16(1):18-31. DOI: 10.1016/j.cmet.2012.06.002

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Lodeiro, M.F., Uchida, A., Bestwick, M., Moustafa, I.M., Arnold, J.J., Shadel, G.S., Cameron, C.E. Transcription from the second heavy-strand promoter of human mtDNA is repressed by transcription factor A in vitro. (2012) Proceedings of the National Academy of Sciences of the United States of America. 109(17):6513-8. DOI: 10.1073/pnas.1118710109

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Schroeder, E.A., Shadel, G.S. Alternative mitochondrial fuel extends life span. (2012) Cell Metabolism. 15(4):417-8. DOI: 10.1016/j.cmet.2012.03.011

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Raimundo, N., Song, L., Shutt, T.E., McKay, S.E., Cotney, J., Guan, M.X., Gilliland, T.C., Hohuan, D., Santos-Sacchi, J., Shadel, G.S. Mitochondrial stress engages E2F1 apoptotic signaling to cause deafness. (2012) Cell. 148(4):716-26. DOI: 10.1016/j.cell.2011.12.027

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Woo, D.K., Green, P.D., Santos, J.H., D'Souza, A.D., Walther, Z., Martin, W.D., Christian, B.E., Chandel, N.S., Shadel, G.S. Mitochondrial genome instability and ROS enhance intestinal tumorigenesis in APC(Min/+) mice. (2012) Am. J. Pathol. 180(1):24-31. DOI: 10.1016/j.ajpath.2011.10.003

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Malarkey, C.S., Bestwick, M., Kuhlwilm, J.E., Shadel, G.S., Churchill, M.E. Transcriptional activation by mitochondrial transcription factor A involves preferential distortion of promoter DNA. (2012) Nucleic Acids Research. 40(2):614-24. DOI: 10.1093/nar/gkr787

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2011

Liu, L., Sanosaka, M., Lei, S., Bestwick, M.L., Frey, J.H., Surovtseva, Y.V., Shadel, G.S., Cooper, M.P. LRP130 protein remodels mitochondria and stimulates fatty acid oxidation. (2011) Journal of Biological Chemistry. 286(48):41253-64. DOI: 10.1074/jbc.M111.276121

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Surovtseva, Y.V., Shutt, T.E., Cotney, J., Cimen, H., Chen, S.Y., Koc, E.C., Shadel, G.S. Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription. (2011) Proceedings of the National Academy of Sciences of the United States of America. 108(44):17921-6. DOI: 10.1073/pnas.1108852108

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Baysal, B.E., McKay, S.E., Kim, Y.J., Zhang, Z., Alila, L., Willett-Brozick, J.E., Pacak, K., Kim, T.H., Shadel, G.S. Genomic imprinting at a boundary element flanking the SDHD locus. (2011) Human Molecular Genetics. 20(22):4452-61. DOI: 10.1093/hmg/ddr376

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Raimundo, N., Baysal, B.E., Shadel, G.S. Revisiting the TCA cycle: signaling to tumor formation. (2011) Trends in Molecular Medicine. 17(11):641-9. DOI: 10.1016/j.molmed.2011.06.001

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Pan, Y., Schroeder, E.A., Ocampo, A., Barrientos, A., Shadel, G.S. Regulation of yeast chronological life span by TORC1 via adaptive mitochondrial ROS signaling. (2011) Cell Metabolism. 13(6):668-78. DOI: 10.1016/j.cmet.2011.03.018

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West, A.P., Shadel, G.S., Ghosh, S. Mitochondria in innate immune responses. (2011) Nature Reviews. Immunology. 11(6):389-402. DOI: 10.1038/nri2975

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Chatenay-Lapointe, M., Shadel, G.S. Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p. (2011) PLOS One. 6(5):e20441. DOI: 10.1371/journal.pone.0020441

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West, A.P., Brodsky, I.E., Rahner, C., Woo, D.K., Erdjument-Bromage, H., Tempst, P., Walsh, M.C., Choi, Y., Shadel, G.S., Ghosh, S. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. (2011) Nature. 472(7344):476-80. DOI: 10.1038/nature09973

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Shutt, T.E., Bestwick, M., Shadel, G.S. The core human mitochondrial transcription initiation complex: It only takes two to tango. (2011) Transcription. 2(2):55-59. DOI: 10.4161/trns.2.2.14296

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Woo, D.K., Shadel, G.S. Mitochondrial stress signals revise an old aging theory. (2011) Cell. 144(1):11-2. DOI: 10.1016/j.cell.2010.12.023

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2010

Lee, H.Y., Choi, C.S., Birkenfeld, A.L., Alves, T.C., Jornayvaz, F.R., Jurczak, M.J., Zhang, D., Woo, D.K., Shadel, G.S., Ladiges, W., Rabinovitch, P.S., Santos, J.H., Petersen, K.F., Samuel, V.T., Shulman, G.I. Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance. (2010) Cell Metabolism. 12(6):668-74. DOI: 10.1016/j.cmet.2010.11.004

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Chatenay-Lapointe, M., Shadel, G.S. Stressed-out mitochondria get MAD. (2010) Cell Metabolism. 12(6):559-60. DOI: 10.1016/j.cmet.2010.11.018

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Shutt, T.E., Lodeiro, M.F., Cotney, J., Cameron, C.E., Shadel, G.S. Core human mitochondrial transcription apparatus is a regulated two-component system in vitro. (2010) Proceedings of the National Academy of Sciences of the United States of America. 107(27):12133-8. DOI: 10.1073/pnas.0910581107

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Shutt, T.E., Shadel, G.S. A compendium of human mitochondrial gene expression machinery with links to disease. (2010) Environmental and Molecular Mutagenesis. 51(5):360-79. DOI: 10.1002/em.20571

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2009

Pawar, V., Jingjing, L., Patel, N., Kaur, N., Doetsch, P.W., Shadel, G.S., Zhang, H., Siede, W. Checkpoint kinase phosphorylation in response to endogenous oxidative DNA damage in repair-deficient stationary-phase Saccharomyces cerevisiae. (2009) Mech. Ageing Dev. 130(8):501-8. DOI: 10.1016/j.mad.2009.06.002

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Shadel, G.S., Pan, Y. Multi-faceted regulation of mitochondria by TOR. (2009) Cell Cycle. 8(14):2143. DOI: 10.4161/cc.8.14.9117


Cotney, J., McKay, S.E., Shadel, G.S. Elucidation of separate, but collaborative functions of the rRNA methyltransferase-related human mitochondrial transcription factors B1 and B2 in mitochondrial biogenesis reveals new insight into mate (2009) Human Molecular Genetics. 18(14):2670-82. DOI: 10.1093/hmg/ddp208

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Griffiths, L.M., Doudican, N.A., Shadel, G.S., Doetsch, P.W. Mitochondrial DNA oxidative damage and mutagenesis in Saccharomyces cerevisiae. (2009) Methods in Molecular Biology. 554:267-86. DOI: 10.1007/978-1-59745-521-3_17

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Crouser, E.D., Shao, G., Julian, M.W., Macre, J.E., Shadel, G.S., Tridandapani, S., Huang, Q., Wewers, M.D. Monocyte activation by necrotic cells is promoted by mitochondrial proteins and formyl peptide receptors. (2009) Critical Care Medicine. 37(6):2000-9. DOI: 10.1097/CCM.0b013e3181a001ae

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Suissa, S., Wang, Z., Poole, J., Wittkopp, S., Feder, J., Shutt, T.E., Wallace, D.C., Shadel, G.S., Mishmar, D. Ancient mtDNA genetic variants modulate mtDNA transcription and replication. (2009) PLOS Genetics. 5(5):e1000474. DOI: 10.1371/journal.pgen.1000474

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Lebedeva, M.A., Eaton, J.S., Shadel, G.S. Loss of p53 causes mitochondrial DNA depletion and altered mitochondrial reactive oxygen species homeostasis. (2009) Biochimica et Biophysica Acta. 1787(5):328-34. DOI: 10.1016/j.bbabio.2009.01.004

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Raimundo, N., Shadel, G.S. A "radical" mitochondrial view of autophagy-related pathology. (2009) Aging. 1(4):354-6. DOI: 10.18632/aging.100037


Tal, M.C., Sasai, M., Lee, H.K., Yordy, B., Shadel, G.S., Iwasaki, A. Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling. (2009) Proceedings of the National Academy of Sciences of the United States of America. 106(8):2770-5. DOI: 10.1073/pnas.0807694106

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Pan, Y., Shadel, G.S. Extension of chronological life span by reduced TOR signaling requires down-regulation of Sch9p and involves increased mitochondrial OXPHOS complex density. (2009) Aging. 1(1):131-45. DOI: 10.18632/aging.100016

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2008

Shadel, G.S. Into the wild frontier of mitochondrial disease and aging. (2008) Methods. 46(4):239-40. DOI: 10.1016/j.ymeth.2008.10.027


Bonawitz, N.D., Chatenay-Lapointe, M., Wearn, C.M., Shadel, G.S. Expression of the rDNA-encoded mitochondrial protein Tar1p is stringently controlled and responds differentially to mitochondrial respiratory demand and dysfunction. (2008) Current Genetics. 54(2):83-94. DOI: 10.1007/s00294-008-0203-0

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Shadel, G.S. Expression and maintenance of mitochondrial DNA: new insights into human disease pathology. (2008) Am. J. Pathol. 172(6):1445-56. DOI: 10.2353/ajpath.2008.071163

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Schleicher, M., Shepherd, B.R., Suarez, Y., Fernandez-Hernando, C., Yu, J., Pan, Y., Acevedo, L.M., Shadel, G.S., Sessa, W.C. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. (2008) Journal of Cell Biology. 180(1):101-12. DOI: 10.1083/jcb.200706072

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2007

Eaton, J.S., Lin, Z.P., Sartorelli, A.C., Bonawitz, N.D., Shadel, G.S. Ataxia-telangiectasia mutated kinase regulates ribonucleotide reductase and mitochondrial homeostasis. (2007) Journal of Clinical Investigation. 117(9):2723-34. DOI: 10.1172/JCI31604

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Lebedeva, M.A., Shadel, G.S. Cell cycle- and ribonucleotide reductase-driven changes in mtDNA copy number influence mtDNA Inheritance without compromising mitochondrial gene expression. (2007) Cell Cycle. 6(16):2048-57. DOI: 10.4161/cc.6.16.4572

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Bonawitz, N.D., Shadel, G.S. Rethinking the mitochondrial theory of aging: the role of mitochondrial gene expression in lifespan determination. (2007) Cell Cycle. 6(13):1574-8. DOI: 10.4161/cc.6.13.4457

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Cotney, J., Wang, Z., Shadel, G.S. Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression. (2007) Nucleic Acids Research. 35(12):4042-54. DOI: 10.1093/nar/gkm424

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Bonawitz, N.D., Chatenay-Lapointe, M., Pan, Y., Shadel, G.S. Reduced TOR signaling extends chronological life span via increased respiration and upregulation of mitochondrial gene expression. (2007) Cell Metabolism. 5(4):265-77. DOI: 10.1016/j.cmet.2007.02.009

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Wang, Z., Cotney, J., Shadel, G.S. Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression. (2007) Journal of Biological Chemistry. 282(17):12610-8. DOI: 10.1074/jbc.M700461200

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Zhang, H., Luo, Y., Zhang, W., He, Y., Dai, S., Zhang, R., Huang, Y., Bernatchez, P., Giordano, F.J., Shadel, G., Sessa, W.C., Min, W. Endothelial-specific expression of mitochondrial thioredoxin improves endothelial cell function and reduces atherosclerotic lesions. (2007) Am. J. Pathol. 170(3):1108-20. DOI: 10.2353/ajpath.2007.060960

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Lin, Z.P., Belcourt, M.F., Carbone, R., Eaton, J.S., Penketh, P.G., Shadel, G.S., Cory, J.G., Sartorelli, A.C. Excess ribonucleotide reductase R2 subunits coordinate the S phase checkpoint to facilitate DNA damage repair and recovery from replication stress. (2007) Biochem. Pharmacol. 73(6):760-72. DOI: 10.1016/j.bcp.2006.11.014

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Shutt, T.E., Shadel, G.S. Expanding the mitochondrial interactome. (2007) Genome Biology. 8(2):203. DOI: 10.1186/gb-2007-8-2-203

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2006

Bonawitz, N.D., Clayton, D.A., Shadel, G.S. Initiation and beyond: multiple functions of the human mitochondrial transcription machinery. (2006) Molecular Cell. 24(6):813-25. DOI: 10.1016/j.molcel.2006.11.024

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Cotney, J., Shadel, G.S. Evidence for an early gene duplication event in the evolution of the mitochondrial transcription factor B family and maintenance of rRNA methyltransferase activity in human mtTFB1 and mtTFB2. (2006) J. Mol. Evol. 63(5):707-17. DOI: 10.1007/s00239-006-0075-1

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Bonawitz, N.D., Rodeheffer, M.S., Shadel, G.S. Defective mitochondrial gene expression results in reactive oxygen species-mediated inhibition of respiration and reduction of yeast life span. (2006) Molecular and Cellular Biology. 26(13):4818-29. DOI: 10.1128/MCB.02360-05

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2005

O'Rourke, T.W., Doudican, N.A., Zhang, H., Eaton, J.S., Doetsch, P.W., Shadel, G.S. Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. (2005) Gene. 354:86-92. DOI: 10.1016/j.gene.2005.03.031

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Shadel, G.S. Mitochondrial DNA, aconitase 'wraps' it up. (2005) Trends in Biochemical Sciences. 30(6):294-6. DOI: 10.1016/j.tibs.2005.04.007

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Doudican, N.A., Song, B., Shadel, G.S., Doetsch, P.W. Oxidative DNA damage causes mitochondrial genomic instability in Saccharomyces cerevisiae. (2005) Molecular and Cellular Biology. 25(12):5196-204. DOI: 10.1128/MCB.25.12.5196-5204.2005

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Taylor, S.D., Zhang, H., Eaton, J.S., Rodeheffer, M.S., Lebedeva, M.A., O'rourke, T.W., Siede, W., Shadel, G.S. The conserved Mec1/Rad53 nuclear checkpoint pathway regulates mitochondrial DNA copy number in Saccharomyces cerevisiae. (2005) Molecular Biology of the Cell. 16(6):3010-8. DOI: 10.1091/mbc.e05-01-0053

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2004

Shadel, G.S. Coupling the mitochondrial transcription machinery to human disease. (2004) Trends in Genetics. 20(10):513-9. DOI: 10.1016/j.tig.2004.08.005

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Shadel, G.S. A dual-function mitochondrial transcription factor tunes out deafness. (2004) Molecular Genetics and Metabolism. 82(1):1-3. DOI: 10.1016/j.ymgme.2004.02.003


2003

McCulloch, V., Shadel, G.S. Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity. (2003) Molecular and Cellular Biology. 23(16):5816-24.

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Rodeheffer, M.S., Shadel, G.S. Multiple interactions involving the amino-terminal domain of yeast mtRNA polymerase determine the efficiency of mitochondrial protein synthesis. (2003) Journal of Biological Chemistry. 278(20):18695-701. DOI: 10.1074/jbc.M301399200

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Seidel-Rogol, B.L., McCulloch, V., Shadel, G.S. Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop. (2003) Nature Genetics. 33(1):23-4. DOI: 10.1038/ng1064

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2002

Coelho, P.S., Bryan, A.C., Kumar, A., Shadel, G.S., Snyder, M. A novel mitochondrial protein, Tar1p, is encoded on the antisense strand of the nuclear 25S rDNA. (2002) Genes & Development. 16(21):2755-60. DOI: 10.1101/gad.1035002

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O'Rourke, T.W., Doudican, N.A., Mackereth, M.D., Doetsch, P.W., Shadel, G.S. Mitochondrial dysfunction due to oxidative mitochondrial DNA damage is reduced through cooperative actions of diverse proteins. (2002) Molecular and Cellular Biology. 22(12):4086-93.

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Seidel-Rogol, B.L., Shadel, G.S. Modulation of mitochondrial transcription in response to mtDNA depletion and repletion in HeLa cells. (2002) Nucleic Acids Research. 30(9):1929-34.

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McCulloch, V., Seidel-Rogol, B.L., Shadel, G.S. A human mitochondrial transcription factor is related to RNA adenine methyltransferases and binds S-adenosylmethionine. (2002) Molecular and Cellular Biology. 22(4):1116-25.

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Bryan, A.C., Rodeheffer, M.S., Wearn, C.M., Shadel, G.S. Sls1p is a membrane-bound regulator of transcription-coupled processes involved in Saccharomyces cerevisiae mitochondrial gene expression. (2002) Genetics. 160(1):75-82.

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2001

Shadel, G.S., Seidel-Rogol, B.L. Diagnostic assays for defects in mtDNA replication and transcription in yeast and humans. (2001) Methods in Cell Biology. 80:465-79. DOI: 10.1016/S0091-679X(06)80023-3


Seidel-Rogol, B.L., Shadel, G.S. Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells. (2001) Methods in Cell Biology. 65:413-27. DOI: 10.1016/s0091-679x(01)65024-6


Rodeheffer, M.S., Boone, B.E., Bryan, A.C., Shadel, G.S. Nam1p, a protein involved in RNA processing and translation, is coupled to transcription through an interaction with yeast mitochondrial RNA polymerase. (2001) Journal of Biological Chemistry. 276(11):8616-22. DOI: 10.1074/jbc.M009901200

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2000

Devine, J.H., Shadel, G.S. Assay of autoinducer activity with luminescent Escherichia coli sensor strains harboring a modified Vibrio fischeri lux regulon. (2000) Meth. Enzymol. 305:279-87. DOI: 10.1016/s0076-6879(00)05494-x


Shadel, G.S., Buckenmeyer, G.A., Clayton, D.A., Schmitt, M.E. Mutational analysis of the RNA component of Saccharomyces cerevisiae RNase MRP reveals distinct nuclear phenotypes. (2000) Gene. 245(1):175-84. DOI: 10.1016/s0378-1119(00)00013-5

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1999

Shadel, G.S. Yeast as a model for human mtDNA replication. (1999) American Journal of Human Genetics. 65(5):1230-7. DOI: 10.1086/302630


Wang, Y., Shadel, G.S. Stability of the mitochondrial genome requires an amino-terminal domain of yeast mitochondrial RNA polymerase. (1999) Proceedings of the National Academy of Sciences of the United States of America. 96(14):8046-51. DOI: 10.1073/pnas.96.14.8046

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1997

Shadel, G.S., Clayton, D.A. Mitochondrial DNA maintenance in vertebrates. (1997) Annual Review of Biochemistry. 66:409-35. DOI: 10.1146/annurev.biochem.66.1.409

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1996

Sitnikov, D.M., Shadel, G.S., Baldwin, T.O. Autoinducer-independent mutants of the LuxR transcriptional activator exhibit differential effects on the two lux promoters of Vibrio fischeri. (1996) Molecular & General Genetics. 252(5):622-5. DOI: 10.1007/bf02172408

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Shadel, G.S., Clayton, D.A. Isolation and characterization of vertebrate mitochondrial transcription factor A homologs. (1996) Meth. Enzymol. 264:149-58. DOI: 10.1016/s0076-6879(96)64016-6


Shadel, G.S., Clayton, D.A. Mapping promoters in displacement-loop region of vertebrate mitochondrial DNA. (1996) Meth. Enzymol. 264:139-48. DOI: 10.1016/s0076-6879(96)64015-4


Carrodeguas, J.A., Yun, S., Shadel, G.S., Clayton, D.A., Bogenhagen, D.F. Functional conservation of yeast mtTFB despite extensive sequence divergence. (1996) Gene Expr. 6(4):219-30.

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1995

Dairaghi, D.J., Shadel, G.S., Clayton, D.A. Human mitochondrial transcription factor A and promoter spacing integrity are required for transcription initiation. (1995) Biochimica et Biophysica Acta. 1271(1):127-34. DOI: 10.1016/0925-4439(95)00019-z

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Dairaghi, D.J., Shadel, G.S., Clayton, D.A. Addition of a 29 residue carboxyl-terminal tail converts a simple HMG box-containing protein into a transcriptional activator. (1995) Journal of Molecular Biology. 249(1):11-28. DOI: 10.1006/jmbi.1995.9889

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Shadel, G.S., Clayton, D.A. A Saccharomyces cerevisiae mitochondrial transcription factor, sc-mtTFB, shares features with sigma factors but is functionally distinct. (1995) Molecular and Cellular Biology. 15(4):2101-8. DOI: 10.1128/mcb.15.4.2101

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1993

Shadel, G.S., Clayton, D.A. Mitochondrial transcription initiation. Variation and conservation. (1993) Journal of Biological Chemistry. 268(22):16083-6.


1992

Shadel, G.S., Baldwin, T.O. Positive autoregulation of the Vibrio fischeri luxR gene. LuxR and autoinducer activate cAMP-catabolite gene activator protein complex-independent and -dependent luxR transcription. (1992) Journal of Biological Chemistry. 267(11):7696-702.

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Shadel, G.S., Baldwin, T.O. Identification of a distantly located regulatory element in the luxD gene required for negative autoregulation of the Vibrio fischeri luxR gene. (1992) Journal of Biological Chemistry. 267(11):7690-5.

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1991

Shadel, G.S., Baldwin, T.O. The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. (1991) J. Bacteriol. 173(2):568-74. DOI: 10.1128/jb.173.2.568-574.1991

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1990

Shadel, G.S., Young, R., Baldwin, T.O. Use of regulated cell lysis in a lethal genetic selection in Escherichia coli: identification of the autoinducer-binding region of the LuxR protein from Vibrio fischeri ATCC 7744. (1990) J. Bacteriol. 172(7):3980-7. DOI: 10.1128/jb.172.7.3980-3987.1990

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Shadel, G.S., Devine, J.H., Baldwin, T.O. Control of the lux regulon of Vibrio fischeri. (1990) J. Biolumin. Chemilumin. 5(2):99-106. DOI: 10.1002/bio.1170050205

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1989

Devine, J.H., Shadel, G.S., Baldwin, T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. (1989) Proceedings of the National Academy of Sciences of the United States of America. 86(15):5688-92. DOI: 10.1073/pnas.86.15.5688

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Baldwin, T.O., Devine, J.H., Heckel, R.C., Lin, J.W., Shadel, G.S. The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence. (1989) J. Biolumin. Chemilumin. 4(1):326-41. DOI: 10.1002/bio.1170040145

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Education

BS, Chemistry, University of Nevada, Las Vegas
PhD, Texas A&M University
Postdoctoral Fellow, Stanford University