San Diego Nathan Shock Center

Sequencing and Single Cell Analysis

Salk Institute for Biological Studies - San Diego Nathan Shock Center - Sequencing and Single Cell Analysis

Sequencing and Single Cell Analysis


1) 10X Single Cell Sequencing Service

2) Single Cell SMART-Seq® v4 Service

Description:
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA, in a convenient input volume of 1–10 µl. This application provides the benefit of generating cDNA using proprietary SMART® (Switching Mechanism at 5ʹ End of RNA Template) technology. This technology relies on the template switching activity of reverse transcriptases to enrich for full-length cDNAs and to add defined PCR adapters directly to both ends of the first-strand cDNA. This ensures the final cDNA libraries contain the 5ʹ end of the mRNA and maintain a true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, and allows direct cDNA synthesis from intact cells.

3) Visium Spatial Gene Expression (Coming in 2022)

4) Nanostring GeoMx® Digital Spatial Profiler Service (Coming in 2022)