Human Cell Models of Aging
Human Cell Models of Aging
The overarching goal of the SD-NSC Human Cell Models of Aging Core is to create and make available powerful, new human cell-based models to enable studies into the molecular and cellular heterogeneity of human aging. Major activities and resources offered include:
- Generate a bank of fibroblasts and corresponding induced pluripotent stem cells (iPSCs) that represent the breadth of healthy adult chronological age from a human cohort extensively phenotyped for key physical and functional measures of biological age.
- Create induced cell models that maintain age-related phenotypes. Unlike iPSC-based reprogramming processes that can erase epigenetic and transcriptional signatures of aging, direct-conversion approaches can better maintain molecular signatures of aging in resultant cell types. This makes direct-induction protocols particularly well-suited for creating cellular models of human aging.
- Drive technology development to create novel organoid and hybrid-cell models to investigate the role of cellular heterogeneity in human tissue and organ aging. This will be iPSC-derived organoids initially, but over time will involve integration of directly converted cells that maintain aging signatures.
- Distribute protocols and key reagents relevant for creating induced cell types and organoid models.
Protocols
- Preparing Matrigel Aliquots, enhanced version
- Preparing Matrigel coated plates, written version, enhanced version
- Passaging hPSC using Versene EDTA, written version, enhanced version
- Maintenance of Primary Human Dermal Fibroblast, written version
- Thawing Human Pluripotent Stem Cells in Feeder-Free Conditions, written version
- Freezing Human Pluripotent Stem Cells in Feeder-Free Conditions, written version
SD-NSC Cell lines
Fibroblasts are now available to aging researchers upon request and approval:
- Follow this link for an updated list of the cell lines currently available.
- If you are interested in using the cells you may:
- Apply to our SD-NSC Pilot Grant Program (deadline Aug 22, 2023).
- Request them anytime during the year contacting Jenn Page
In any case, you will need to submit a cell line request by completing: SD-NSC Cell line request form
Personnel and contact information
Jenn Page, Stem Cell Core Director
jepage@salk.edu
