October 31, 2019

Unlocking the black box of embryonic development

International collaboration improves method for culturing primate embryos to learn more about human development

Salk News

Unlocking the black box of embryonic development

International collaboration improves method for culturing primate embryos to learn more about human development

LA JOLLA—Little is known about the molecular and cellular events that occur during early embryonic development in primate species. Now, an internationally renowned team of scientists in China and the United States has created a method to allow primate embryos to grow in the laboratory longer than ever before, enabling the researchers to obtain molecular details of key developmental processes for the first time. This research, while done in nonhuman primate cells, can have direct implications for early human development.

Pictured is Day 17 of a cultured primate embryo; the various colors indicate markers of cellular differentiation (specialization).
Pictured is Day 17 of a cultured primate embryo; the various colors indicate markers of cellular differentiation (specialization).

Click here for a high-resolution image.

Credit: Weizhi Ji/Kunming University of Science and Technology

The findings, published in Science on October 31, 2019, provide valuable insight into early embryonic development and potentially can help inform approaches to advance regenerative medicine in humans.

“Our study provides a first look into this black box of early development,” says Juan Carlos Izpisua Belmonte, co-corresponding author and a professor in Salk’s Gene Expression Laboratory. “We can now observe how cells progress through each embryonic stage and what factors they need to develop, which will aid in creating better options for the generation of a variety of cells and tissues.”

“To understand cellular and molecular mechanisms underlying primate gastrulation, we began monkey embryo culture experiments in China three years ago. Because of the team’s long-standing expertise on the systematic study of nonhuman primates and well-established reproductive research systems, such as an in vitro fertilization platform, we succeeded in achieving our goals. This can help to shed light on previously unknown aspects of human post-implantation development.” says Weizhi Ji, co-corresponding author, professor and dean of the Institute of Primate Translational Medicine of Kunming University of Science and Technology, in China.

The scientists wanted to study an early developmental milestone called gastrulation, which occurs when a developing embryo transforms into a multilayered structure, called the gastrula, from which all future tissues and organs will be derived. One layer will become the lungs, gastrointestinal tract and liver; another will become the heart, muscles and reproductive organs; and a third will become the skin and nervous system. Yet, scientists did not know the molecular and cellular drivers of this process in primates, largely due to limited access to early embryos.

Juan Carlos Izpisua Belmonte
Juan Carlos Izpisua Belmonte

Click here for a high-resolution image.

Credit: Salk Institute

“Our goal was to culture a primate embryo from an early timepoint in order to study the process of development,” says Jun Wu, a co-author of the paper and assistant professor at University of Texas Southwestern Medical Center. “We wanted to monitor the embryos every day to observe their shape, size and migration patterns as well as how they generate different types of cells during early primate development.”

To better study this critical transformation, the scientists modified a previously established embryo culture protocol to allow an early primate embryo to develop in laboratory conditions for up to twenty days; previously researchers had only been able to maintain cultured primate embryos prior to the second week of gestation. Using the new protocol, the team found that the cells within cultured embryos exhibited clear developmental trajectories towards each layer of the gastrula, and the results revealed some of the molecular details required for this growth. The data could also be used as a resource to aid in extending the cultured embryo duration past twenty days in order to better study stem- cell differentiation (specialization).

“These results illuminate some of the regulation networks and signaling pathways that are crucial to development in primates,” says Izpisua Belmonte. “This system provides a foundation and resource for developing better strategies to examine early primate development in both health and disease, in the laboratory.”

Other authors included Yuyu Niu, Nianqin Sun, Chang Li, Chenyang Si, Jinkuan Wei, Yu Kang, Wei Si, Hong Wang, E Zhang, Lu Zhao, Ziwei Li and Tao Tan of Kunming University of Science and Technology; and Ying Lei, Xi Dai, Chuanyu Liu, Zhihao Huang and Longqi Liu of BGI-Shenzhen.

The work was funded by the National Key Research and Development Program (2016YFA0101401), the National Natural Science Foundation of China (81760271), Major Science and Technology Projects of Yunnan Province (2017ZF028) and Key Projects of Basic Research Program in Yunnan Province (2017FA010). Work in the laboratory of Juan Carlos Izpisua Belmonte was supported by The Moxie Foundation.

DOI: 10.1126/science.aaw5754





Dissecting primate early post-implantation development using long


Yuyu Niu, Nianqin Sun, Chang Li, Ying Lei, Zhihao Huang, Jun Wu, Chenyang Si, Xi Dai, Chuanyu Liu, Jinkuan Wei, Longqi Liu, Yu kang, Wei Si, Hong Wang, E Zhang, Lu Zhao, Ziwei Li, Juan Carlos Izpisúa Belmonte, Weizhi Ji and Tao Tan

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