August 27, 2019

New target for autoimmune disease could enable therapies with fewer side effects

Salk scientists find how to block inflammatory molecules in mouse model of multiple sclerosis

Salk News


New target for autoimmune disease could enable therapies with fewer side effects

Salk scientists find how to block inflammatory molecules in mouse model of multiple sclerosis

LA JOLLA—Your immune system comes ready for battle against bacteria, viruses, fungi and even cancer. But in cases of autoimmune disease, the immune system’s superpowers turn it into a supervillain. Now, Salk Institute scientists have discovered a way to stop certain immune system cells from mistakenly attacking the body. Their findings, published the week of August 26, 2019, in the journal Proceedings of the National Academy of Sciences, suggest a new way to target Th17 helper T cells, a type of immune cell that produces interleukin 17, a molecule known to be at the root of autoimmune diseases such as multiple sclerosis, rheumatoid arthritis and psoriasis. Previous efforts targeting Th17 helper T cells have had limited success.

“In an autoimmune disease situation, the immune system turns its weapons on itself,” says Associate Professor Ye Zheng, of Salk’s NOMIS Center for Immunobiology and Microbial Pathogenesis and co-senior author of the study.

“Autoimmune diseases affect around 1 in 30 people. This research opens the door to treating these patients without triggering harmful side effects,” says co-senior author and director of Salk’s Gene Expression Laboratory Ronald Evans, who holds the March of Dimes Chair in Molecular and Developmental Biology and is a Howard Hughes Medical investigator.

The team of researchers, including first author Christina Chang, used a mouse model of multiple sclerosis to investigate the overproduction of interleukin 17 in Th17 helper T cells. These immune cells normally work to cure infections, with interleukin 17 sending an inflammation signal to help kick off the immune response. However, sometimes the immune cells become hyperactive and start churning out too much interleukin 17, damaging nearby tissues and kicking off the autoimmune disease process.

Drug treatments can slow interleukin 17 production, but they also work as powerful immunosuppressants, blocking the body’s overall ability to fight infections. For the new study, the Salk scientists wondered if there was a way to block only interleukin 17 production without harming immune system function.

The team uncovered a way to use a molecule called REV-ERB to suppress interleukin 17 production. REV-ERB binds to a site on the Th17 helper T cells that is normally occupied by a molecule called ROR, responsible for interleukin 17 production. By taking over the same binding location, REV-ERB competes with ROR. “ROR is a gas pedal that drives these helper T cells, but REV-ERB is like the brakes,” says Zheng.

The researchers then showed they were able to chemically activate REV-ERB and delay the onset and slow the progression of symptoms of experimental autoimmune encephalomyelitis, the mouse model of multiple sclerosis.

More work is needed, but the scientists say targeting this particular pathway offers a way to tamp down interleukin 17 expression without affecting the rest of the immune system.

“It’s an early stage, but it’s certainly encouraging,” adds Michael Downes, co-author of the study and a senior staff scientist in Salk’s Gene Expression Laboratory.

Going forward, the scientists plan to study the role of REV-ERB in models of rheumatoid arthritis and psoriasis.

Other authors included Xuan Zhao, Yuqiong Liang, Han Cho, Mathias Leblanc, Annette R. Atkins and Ruth T. Yu of Salk, Chin-San Loo, Sagar P. Bapat of Salk and the University of California, San Diego; Laura A. Solt and Theodore M. Kamenecka of Scripps Research; and Thomas P. Burris of Washington University School of Medicine and St. Louis College of Pharmacy.

The work was funded by the National Institutes of Health (DK096828, T32 GM007198, HL088093, HL105278, AI107027, OD023689, P42ES010337), the H.A. and Mary K. Chapman Charitable Trust, the Albert G. and Olive H. Schlink Foundation, the Leona M. and Harry B. Helmsley Charitable Trust (grant #2017PG-MED001), Ipsen/Biomeasure, Fondation Leducq, the NOMIS Foundation, the Rita Allen Foundation and the National Multiple Sclerosis Society (RG4978-A-2). This work was also supported by National Cancer Institute funded Salk Institute Cancer Center core facilities (CA014195).

DOI: 10.1073/pnas.1907563116

PUBLICATION INFORMATION

JOURNAL

Proceedings of the National Academy of Sciences

TITLE

The nuclear receptor REV-ERB modulates Th17 cell-mediated autoimmune disease

AUTHORS

Christina Chang, Chin-San Loo, Xuan Zhao, Laura A. Solt, Yuqiong Liang, Sagar P. Bapat, Han Cho, Theodore M. Kamenecka, Mathias Leblanc, Annette R. Atkins, Ruth T. Yu, Michael Downes, Thomas P. Burris, Ronald M. Evans, and Ye Zheng

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