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Doludda, B., Barde, W., D'Egidio, F., Fitzsimons, C.P., Frisén, J., Gage, F.H., Jessberger, S., Lazarov, O., Lie, D.C., Lucassen, P.J., de Lucia, C., Salta, E., Song, H., Song, J., Thuret, S., Toda, T., Kempermann, G. Neurogénesis adulta: Nuevas neuronas, nuevas oportunidades. (2026) Células Madre. 33(3):382-392. DOI: 10.1016/j.stem.2026.01.014

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Duff, M.K., Li, M.J., Nimmerjahn, A. Astrocyte Regulation of Spinal Circuit Function. (2026) J Neurochem. 170(2):e70366. DOI: 10.1111/jnc.70366

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The spinal cord stands as a crucial nexus in the central nervous system (CNS), integrating and modulating signals that ultimately shape our everyday interactions with the world. Its gray matter is arranged into discrete laminae spanning the dorsal-ventral axis that encompass circuit-specific modalities. Concurrently, extensive interconnected interneuron networks within and between these laminae confer remarkable flexibility in the behavioral outputs for a given input. The flexibility of spinal cord information processing in light of its organized architecture makes it a particularly intriguing region to explore the neuronal computations underlying behaviors, particularly as they relate to neurological dysfunction. At the same time, astrocytes engage in highly dynamic interactions with underlying neuronal circuitries, suggesting they may add another dimension to spinal cord information processing. Technical limitations specific to the spinal cord have long limited our ability to interrogate the relationship between astrocyte-neuron interactions and ongoing spinal cord function. In this review, we highlight emerging insights-particularly those from recent in vivo studies-that illustrate astrocytes actively shape spinal cord behavioral outputs in both health and disease. We briefly review the spinal cord's neuronal organization to provide a structural foundation for assessing the relative spatial relationship between astrocyte and neuron activity as it relates to different spinal cord outputs. Within this architectural framework, we review growing evidence that spinal cord astrocytes respond to activity associated with spinal cord function and, in turn, modulate underlying neuronal circuits to alter future behavioral outputs. Moreover, we propose an overall conceptual framework for understanding circuit-specific spinal cord modulations through the lens of astrocyte-neuron interactions and underscore how it can be leveraged to uncover novel ways of targeting spinal cord disease states. Finally, we put forth key outstanding questions related to this conceptual framework and emphasize the technological advances that will facilitate future studies addressing them.

Tsuda, B., Pate, S.C., Tye, K.M., Siegelmann, H.T., Sejnowski, T.J. Neuromodulators Generate Multiple Context-Relevant Behaviors in Recurrent Neural Networks. (2026) Computación neuronal.:1-36 DOI: 10.1162/NECO.a.1489

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Keller, T.A., Muller, L., Sejnowski, T.J., Welling, M. A Spatiotemporal Perspective on Dynamical Computation in Neural Information Processing Systems. (2026) ArXiv.

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Argueti-Ostrovsky, S., Lim, S.M., Arogundade, O.A., Diaz-Garcia, S., Yunisova, G., Meng, A., Hermann, A., Ong, K., Eremenko, E., Bravo-Hernandez, M., Driscoll, S.P., Lee, C.Z., Jiang, X., Stavsky, A., Barel, S., Shani, T., Kahn, J., Pfaff, S.L., Monsonego, A., Marsala, M., Ravits, J., Lagier-Tourenne, C., Israelson, A. Impaired nucleocytoplasmic transport in SOD1-mediated ALS. (2026) Mol Neurodegener. DOI: 10.1186/s13024-026-00930-8

Wagner, M., Chen, Y., Karuvally, A., Cameron, M., Sejnowski, T.J. Balancing Stability and Flow in Hippocampal Networks via Inductive Bias and Learned Symmetry Breaking. (2026) bioRxiv. DOI: 10.64898/2026.02.06.704443

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Sejnowski, T.J. Mecanismos Dinámicos para la Coordinación de la Memoria de Trabajo a Largo Plazo Basados en la Precisión de la Sincronización de Espigas en Neuronas Corticales. (2025) ArXiv.

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En el siglo pasado, la mayoría de los estudios sensoriomotores sobre las neuronas corticales se basaban en las frecuencias medias de disparo. La codificación de frecuencia resulta eficaz para el procesamiento sensoriomotor rápido que tiene lugar en unos pocos segundos. Se sabe mucho menos sobre la memoria de trabajo a largo plazo, con una escala temporal de horas (Ericsson y Kintsch, 1995). El descubrimiento de la precisión milisegundaria del inicio de los picos en las neuronas corticales fue inesperado (Mainen y Sejnowski, 1995). Aún más sorprendente fue la precisión de los picos in vivo, en respuesta a entradas sensoriales que fluctuaban rápidamente, lo que sugiere que los circuitos neuronales podrían, en principio, conservar y manipular la información sensorial a través de la sincronización de los picos. Esto podría respaldar la plasticidad dependiente de la sincronización de los picos (STDP), que se desencadena por la sincronización relativa de los picos entre las neuronas presinápticas y postsinápticas en el rango de los milisegundos. ¿Qué mecanismos de sincronización de los picos podrían regular la STDP in vivo? Se han observado ondas viajeras corticales a través de muchas bandas de frecuencia con alta precisión temporal. Las ondas viajeras tienen frentes de onda que podrían vincular la sincronización de los picos con la STDP. A medida que un frente de onda atraviesa una columna cortical, las sinapsis excitatorias en los dendritos tanto de las células piramidales como de las cestas se estimulan de manera sincrónica. Las células de cesta inhibitorias forman un cáliz sobre los cuerpos celulares piramidales, y el rebote inhibitorio tras una fuerte hiperpolarización transitoria puede desencadenar un potencial de acción de retropropagación, que llega poco después de las entradas excitatorias en los dendritos piramidales. El STDP activado de esta manera podría persistir durante horas, creando una red de segundo nivel. Esta red temporal podría sustentar la memoria de trabajo a largo plazo, una red cognitiva que se superpone a la red sensoriomotora a largo plazo. Por sí solas, las ondas viajeras y el STDP aún no han aportado nuevos conocimientos sobre la función cortical. Juntas, podrían ser responsables de cómo pensamos (Sejnowski, 2025).

Kelsey, M.M.G., Chongtham, A., LaCava, J., Taylor, M.S., Boeke, J.D., Gage, F.H., Seluanov, A., Gorbunova, V., Pereira, A.C., Sedivy, J.M. Activación de Retrotransposones en el Cerebro Envejecido y con Enfermedad de Alzheimer Examinada por Secuenciación de ADN de Lectura Larga Nanopore. (2025) bioRxiv. DOI: 10.64898/2025.12.12.693943

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Pașca, S.P., Arlotta, P., Campbell, P., Charo, A., Evans, J.H., Farahany, N., Gage, F.H., Godfrey-Smith, P., Hyun, I., Kriegstein, A.R., Melloni, L., Ming, G.L., Moreno, J.D., Sugarman, J., Temple, S., Testa, G., Greely, H.T. La necesidad de un esfuerzo global para atender la investigación de organoides y asambloides neuronales humanos. (2025) Ciencia. 390(6773):574-577. DOI: 10.1126/science.aeb1510

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Pinto-Duarte, A., Bogue, K., Sejnowski, T.J., Srinivasan, S. Conservación de la densidad glial y las proporciones de tipos celulares dentro de una región cerebral en mamíferos. (2025) PNAS Nexus. 4(11):pgaf314. DOI: 10.1093/pnasnexus/pgaf314

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Farsheed, A.C., Makhoul, J.T., Chew-Martinez, D., Maldonado, E., Liu, J., Yu, L.T., Gorostieta-Salas, E., Jones, J.R., Gage, F.H., Hartgerink, J.D. Impresión 3D Omnidireccional de Hidrogeles Peptídicos Anisotrópicos de Nano . (2025) bioRxiv. DOI: 10.1101/2025.11.06.687046

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Zhang, H., Nelken, I., Sharpee, T. Análisis del código neural auditivo en paquetes de máxima entropía. (2025) bioRxiv. DOI: 10.1101/2025.11.09.687481

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Klug, J.R., Yan, X., Hoffman, H., Engelhardt, M.D., Osakada, F., Callaway, E.M., Jin, X. Las proyecciones corticales asimétricas a las vías directa e indirecta del cuerpo estriado controlan las acciones de forma distinta. (2025) Elife. 12. DOI: 10.7554/eLife.92992

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Moore, J.D., Bachmann, L.C., McElvain, L.E., Pfaff, S.L., Dulac, C. Una población genéticamente definida de neuronas amigdalofugales promueve la succión y el crecimiento posnatal temprano. (2025) bioRxiv. DOI: 10.1101/2025.10.18.683193

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Yao, M., Praturu, A., Sharpee, T. Incrustación Hiperbólica Multiescala para Jerarquías Celulares en Datos Bioinformáticos a Gran Escala. (2025) bioRxiv. DOI: 10.1101/2025.09.29.679407

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Barykina, N.V., Carey, E.M., Oliinyk, O.S., Mendonça-Gomes, J.M., de Oliveira, S., Nimmerjahn, A., Verkhusha, V.V. Plataforma de nanobodies sintéticos multicolores estabilizados contra antígenos para marcado intersecional e imagenología funcional. (2025) bioRxiv. DOI: 10.1101/2025.10.27.684934

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Virlogeux, A., Bollu, T., Ren, X., Palma, A.M., Goulding, M.D. Los núcleos de la columna dorsal codifican y transmiten las firmas de red de la alodinia mecánica. (2025) Cell Reports. 44(9):116248. DOI: 10.1016/j.celrep.2025.116248

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Esparza-Moltó, P.B., Goswami, A.V., Bozkurt, S., Münch, C., Newman, L.E., Moyzis, A.G., Rojas, G.R., Guan, D., Jones, J.R., Gage, F.H., Shadel, G.S. Localización de enzimas glucolíticas en mitocondrias dependiente de ROS. (2025) Redox Biol. 86:103812. DOI: 10.1016/j.redox.2025.103812

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Las especies reactivas del oxígeno mitocondrial (mtROS) regulan las vías de señalización celular, pero también provocan estrés oxidativo cuando se desregulan durante el envejecimiento y en condiciones patológicas como las enfermedades neurodegenerativas. La redistribución dinámica de proteínas entre los compartimentos celulares es un mecanismo común para controlar su estabilidad y actividades biológicas. Al dirigir la biotina ligasa BirA∗ hacia la membrana mitocondrial externa en células HEK293, identificamos proteínas cuyo marcaje aumentó o disminuyó en respuesta al tratamiento con menadiona, lo cual concuerda con un cambio dinámico en su localización mitocondrial en respuesta al aumento de la producción de mtROS. Estas proteínas representan candidatos potenciales para futuros estudios sobre la señalización del estrés oxidativo mitocondrial. En este cribado se encontró un subconjunto de enzimas glucolíticas y se confirmó, mediante fraccionamiento mitocondrial e imágenes, que aumentaban su localización en las mitocondrias en respuesta a la menadiona, a pesar de que no se observó ningún cambio en su abundancia general. Los estudios de fraccionamiento submitocondrial concuerdan con la importación de un conjunto de estas enzimas al espacio intermembranario mitocondrial. La localización de las enzimas glucolíticas en las mitocondrias también aumentó en células cultivadas en condiciones de hipoxia o que expresan una d-aminoacido oxidasa dirigida a las mitocondrias (condiciones que inducen un aumento en la producción de ROS mitocondriales), y se inhibió de forma basal en condiciones normales de crecimiento mediante el antioxidante mitocondrial MnTBAP. Por último, los fibroblastos de la enfermedad de Alzheimer primaria también presentaban enzimas glucolíticas asociadas a las mitocondrias que se reducían con los antioxidantes, lo que concuerda con el aumento de mtROS que altera su distribución relativa entre el citoplasma y las mitocondrias. Especulamos que el aumento de la localización mitocondrial de las enzimas glucolíticas es una respuesta adaptativa a los mtROS que altera el flujo de glucosa hacia la vía de las pentosas fosfato antioxidante, crea reservorios reguladores distintos de metabolitos mitocondriales o nuevos circuitos metabólicos, y/o proporciona citoprotección u otras respuestas adaptativas a través de funciones secundarias no relacionadas con su actividad enzimática.

Nakahira, Y., Liu, Q., Deng, X., Sejnowski, T.J., Doyle, J.C. La diversidad libera las limitaciones de los componentes en el control sensoriomotor. (2025) Computación neuronal.:1-24 DOI: 10.1162/neco.a.24

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Praturu, A., Sharpee, T.O. Factorización de Matrices Logística Basada en Distancia. (2025) Computación neuronal.:1-14 DOI: 10.1162/neco.a.25

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Calatayud, C., Fernandez-Carasa, I., Spataro, N., Mussolino, C., Richaud-Patin, Y., Faella, A., Fernández-Santiago, R., Ezquerra, M., Courtin, T., Bandres-Ciga, S., Miguez, A., Canals, J.M., Chiritoiu, M., Malhotra, V., Garrido, A., Marti, M.J., Tolosa, E., Bosch, E., Cathomen, T., Gage, F.H., Raya, A., Consiglio, A. Las variantes genéticas de baja frecuencia en GAK mejoran la función del Golgi y protegen contra la enfermedad de Parkinson. (2025) medRxiv. DOI: 10.1101/2025.08.13.25333123

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Molokanova, E., Zhou, T., Vasupal, P., Cherkas, V.P., Narute, P., Ferraz, M.S.A., Reiss, M., Almenar-Queralt, A., Chaldaiopoulou, G., de Souza, J.S., Hemati, H., Downey, F., Olajide, O.O., Thörn Perez, C., Puppo, F., Mesci, P., Pfaff, S.L., Kireev, D., Muotri, A.R., Savchenko, A. Modulación neuronal no genética con actuadores optoelectrónicos de grafeno para modelos de enfermedades, maduración de células madre y robótica bio-híbrida. (2025) Nature Communications. 16(1):7499. DOI: 10.1038/s41467-025-62637-6

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Baek, G.H., Kim, D., Son, G., Do, H., Yeon, G.B., Lee, M.J., Ji, M., Son, J.H., Ju, M., Ahn, I., Kang, C.S., Lee, H., Choi, S., Suh, J.M., Seo, J., Gage, F.H., Paik, M.J., Park, Y., Kim, D.S., Han, J. Efectos diferenciales del litio en disfunciones metabólicas en astrocitos derivados de pacientes con trastorno bipolar. (2025) Psiquiatría Molecular. DOI: 10.1038/s41380-025-03176-w

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Se han observado alteraciones metabólicas en el cerebro de pacientes con trastorno bipolar (TB), un trastorno neuropsiquiátrico caracterizado por episodios alternantes de manía y depresión. Sin embargo, las contribuciones específicas de las células gliales a estos cambios metabólicos siguen siendo en gran medida desconocidas. En este estudio, investigamos las características metabólicas de los astrocitos inducidos (iAstrocitos) derivados de células madre pluripotentes inducidas de pacientes con TB —clasificados según su respuesta al litio— y de controles sanos. Los análisis transcriptómicos revelaron una expresión desregulada de genes asociados con enfermedades metabólicas en los iAstrocitos de pacientes con TB. En comparación con los iAstrocitos de control, los iAstrocitos de pacientes con TB mostraron una disminución de la respiración mitocondrial, un aumento de la glucólisis y una secreción elevada de lactato, lo que indica una función mitocondrial alterada. Estos defectos se vieron respaldados además por la regulación a la baja de las proteínas del complejo de fosforilación oxidativa y la disminución de los niveles de especies reactivas de oxígeno. Cabe destacar que los iAstrocitos de BD mostraron una acumulación sustancial de gotitas lipídicas (LD), potencialmente como consecuencia de una homeostasis metabólica alterada. El tratamiento con litio redujo los niveles de LD en los iAstrocitos sensibles al litio (Li-R), pero no logró restaurar la respiración mitocondrial ni normalizar la secreción de lactato. En el cocultivo con neuronas humanas, los iAstrocitos no sensibles al litio (Li-NR) mostraron una mayor captación de lípidos derivados de las neuronas y un aumento selectivo de la excitabilidad neuronal. El perfil metabolómico reveló firmas metabólicas distintas entre los iAstrocitos Li-R y Li-NR, lo que sugiere que la respuesta al litio es un eje clave de la heterogeneidad metabólica. En conjunto, nuestros hallazgos identifican la disfunción metabólica específica de los astrocitos como un rasgo característico del TB y revelan funciones divergentes de los iAstrocitos Li-R y Li-NR en la modulación de la función neuronal. La acumulación de LD en los iAstrocitos Li-NR podría servir como indicador funcional para el cribado de fármacos con el fin de identificar tratamientos alternativos para pacientes que no responden al litio.

Da Silva, I.I.N., Ramirez, D., Parylak, S.L., Wallace, L.A., Tucker, J.K., Erberich, J.M., Katariya, R., McDonald, A.H., Gallina, I.S., Tucker, A.L., Burgado, J., Jaeger, B.N., Barron, J.J., Pratt, J.M., Pena, M., Racha, V., Lim, C.K., Fernandes, S., Benassi, S., Randolph-Moore, L., Vadodaria, K.C., Marchetto, M.C., Allen, N.J., Gage, F.H. La inhibición de CK2 suprime la inflamación glial en el cerebro. (2025) bioRxiv. DOI: 10.1101/2025.08.05.668554

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Kang, S.J., Liu, S., Kim, J.H., Kim, D.I., Oh, T.G., Peng, J., Ye, M., Lee, K.F., Evans, R.M., Goulding, M., Han, S. Las neuronas CGRP talámicas definen una vía espinotalámica para el dolor afectivo. (2025) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 122(28):e2505889122. DOI: 10.1073/pnas.2505889122

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Moreira, J.P.C., Carvalho, V.R., Mendes, E.M.A.M., Fallah, A., Sejnowski, T.J., Lainscsek, C., Comstock, L. Un conjunto de datos EEG de acceso abierto para decodificación del habla: explorando el papel de la articulación y la coarticulación. (2025) Datos científicos. 12(1):1017. DOI: 10.1038/s41597-025-05187-2

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Yao, M., Nagamori, A., Campos Maçãs, S., Azim, E., Sharpee, T., Goulding, M., Golomb, D., Gatto, G. La red premotora espinal que impulsa la alternancia flexora y extensora del rascado. (2025) Cell Reports. 44(6):115845. DOI: 10.1016/j.celrep.2025.115845

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Rowekamp, R.J., Sharpee, T.O. Computaciones que sostienen la selectividad de características neuronales a través de etapas de procesamiento. (2025) PLOS Biología Computacional. 21(6):e1013075. DOI: 10.1371/journal.pcbi.1013075

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Liu, Y.N., Wang, Q.W., Lu, S.Y., Shen, W., Guo, C., Xing, Z., Li, C., Sun, S., Sui, S.F., Mi, S., Gage, F.H., Yao, J. El déficit de sinaptotagmina-7 causa hipoactividad de la insulina y contribuye a alteraciones conductuales en ratones. (2025) iCiencia. 28(5):112354. DOI: 10.1016/j.isci.2025.112354

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Rusu, I., Cecere, Z.T., How, J.J., Quach, K.T., Yemini, E., Sharpee, T.O., Chalasani, S.H. Un marco para analizar la actividad neuronal de C. elegans utilizando incrustación hiperbólica multidimensional (2025) bioRxiv. DOI: 10.1101/2021.04.09.439242

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Kirk, L.M., Garcia, G.C., Hanka, D.C., Zatyko, K., Bartol, T., Sejnowski, T.J., Harris, K.M. Las vesículas presinápticas suministran membrana para el agrandamiento del botón axonal durante la LTP. (2025) bioRxiv. DOI: 10.1101/2025.04.29.651313

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Goulding, M., Bollu, T., Büschges, A. Retroalimentación Sensorial y el Control Dinámico del Movimiento. (2025) Revisión Anual de Neurociencia. DOI: 10.1146/annurev-neuro-112723-042229

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Mi, X., Chen, A.B., Duarte, D., Carey, E., Taylor, C.R., Braaker, P.N., Bright, M., Almeida, R.G., Lim, J.X., Ruetten, V.M.S., Wang, Y., Wang, M., Zhang, W., Zheng, W., Reitman, M.E., Huang, Y., Wang, X., Li, L., Deng, H., Shi, S.H., Poskanzer, K.E., Lyons, D.A., Nimmerjahn, A., Ahrens, M.B., Yu, G. Plataforma de análisis de datos rápida, precisa y versátil para la cuantificación de señales moleculares espaciotemporales. (2025) Móvil. DOI: 10.1016/j.cell.2025.03.012

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Bartol, T.M., Ordyan, M., Sejnowski, T.J., Rangamani, P., Kennedy, M.B. Un modelo espacial de autofosforilación de CaMKII predice que la vida útil del phospho-CaMKII después de la inducción de plasticidad sináptica se prolonga enormemente por el atrapamiento de CaM. (2025) Frente en Neurociencia Sináptica. 17:1547948. DOI: 10.3389/fnsyn.2025.1547948

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La potenciación a largo plazo (LTP) es un proceso bioquímico que subyace al aprendizaje en las sinapsis glutamatérgicas excitatorias del sistema nervioso central (SNC). Un factor impulsor temprano y fundamental de la LTP es la autofosforilación de la proteína quinasa II dependiente de Ca/calmodulina (CaMKII), una enzima postsináptica muy abundante. La autofosforilación se inicia por el flujo de Ca a través de los receptores NMDA activados por una fuerte actividad sináptica. Su duración viene determinada en última instancia por el equilibrio entre las tasas de autofosforilación y de desfosforilación por la proteína fosfatasa 1 (PP1). Aquí hemos modelado la autofosforilación y la desfosforilación de la CaMKII durante la actividad sináptica en una sinapsis espinal utilizando MCell4, un programa informático de código abierto para crear modelos estocásticos basados en partículas y espacialmente realistas de la microquímica celular. El modelo integra cuatro modelos detallados anteriores de aspectos separados de la regulación de la actividad del Ca y de la CaMKII en las espinas, cada uno de los cuales incorpora parámetros bioquímicos medidos experimentalmente y ha sido validado con datos experimentales. Validamos el modelo compuesto demostrando que predice con precisión mediciones experimentales previas de los efectos de la activación del receptor NMDA, incluyendo la alta sensibilidad de la inducción de la LTP a la actividad de la fosfatasa y la persistencia de la autofosforilación durante un período de minutos después del final de la estimulación sináptica. A continuación, utilizamos el modelo para investigar aspectos del mecanismo de regulación de la autofosforilación de la CaMKII que son difíciles de medir. Examinamos los efectos del "atrapamiento de CaM", un proceso en el que la afinidad por el Ca/CaM aumenta varios cientos de veces tras la autofosforilación. Descubrimos que el atrapamiento de CaM no aumenta la proporción de subunidades autofosforiladas en las holoenzimas tras un estímulo complejo, como se había hipotetizado anteriormente. En cambio, el atrapamiento de CaM puede prolongar drásticamente la vida útil de la CaMKII autofosforilada a través de un impedimento estérico de la desfosforilación por parte de la proteína fosfatasa 1. Los resultados motivan la medición experimental del grado de supresión de la desfosforilación de la CaMKII por el Ca/CaM unido. El modelo compuesto MCell4 de los efectos bioquímicos de los estímulos complejos en las espinas sinápticas es una nueva y poderosa herramienta para el análisis realista y detallado de los mecanismos de la plasticidad sináptica.

Tucker, J.K., Da Silva, I.I.N., Gage, F.H. El "scaffold hopping" y la modificación de cadenas laterales a partir de un esqueleto de flavona conducen al descubrimiento de potentes inhibidores selectivos de CK2A2 con propiedades favorables para la actividad en el SNC. (2025) Química Bioorgánica y Medicinal. 127:118196. DOI: 10.1016/j.bmc.2025.118196

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Hur, M., Bartol, T., Rangamani, P., Sejnowski, T., Mjolsness, E. Dinamorfología de cabezas de espinas sinápticas a partir de reglas de gramática gráfica para la dinámica de actina. (2025) ArXiv.

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El aprendizaje sináptico tiene un componente morfodinámico por el cual los cambios en el tamaño de la cabeza de las espinas dendríticas (postsinápticas) se asocian con el fortalecimiento o debilitamiento de la conexión sináptica entre dos neuronas, en respuesta a la correlación temporal de las señales locales presinápticas y postsinápticas. A su vez, estos factores morfológicos están determinados por la dinámica del citoesqueleto de actina. En este artículo, utilizamos gramáticas de grafos dinámicos (DGG) implementadas en un sistema de álgebra computacional para modelar cómo las redes de filamentos de actina pueden crecer o encogerse dinámicamente, remodelando la cabeza de la espina. Las gramáticas de grafos dinámicos (DGG) proporcionan una forma bien definida de dar cabida a estructuras de sistemas que cambian dinámicamente, como el citoesqueleto activo representado mediante grafos dinámicos, dentro de la física estadística de no equilibrio bajo la ecuación maestra. Demostramos que las DGG también pueden incorporar fuerzas biofísicas entre objetos conectados por grafos a una escala de tiempo más fina, con reglas cinéticas DGG especializadas que obedecen a restricciones biofísicas de invariancia galileana, conservación del momento y disipación de la energía global conservada. Utilizamos funciones de energía locales al grafo para redes de citoesqueleto que interactúan con membranas, y derivamos reglas DGG a partir de la especialización de la dinámica estocástica disipativa —separada en tipos de reglas de ruido disipativo y térmico— en una colección mutuamente excluyente y exhaustiva de tipos de vecindad locales al grafo para los lados izquierdos de las reglas. Las reglas disipativas comprenden una versión estocástica de la dinámica de descenso por gradiente. Las reglas de ruido térmico utilizan una aproximación gaussiana de cada coordenada de posición para muestrear desplazamientos similares a la fluctuación. Para el modelo de cabeza de espina, diseñamos e implementamos submodelos matemáticos de gramática DGG que incluyen el crecimiento de la red de actina, la mecánica estadística de no equilibrio y la interacción mecánica entre filamentos y membranas para regular la reescritura de los objetos del grafo. Simulamos la biofísica emergente de redes simplificadas de polímeros de actina y sus interacciones con las membranas. Desde una perspectiva biológica, observamos los efectos reguladores de tres proteínas de unión a la actina (ABP) sobre el tamaño de la membrana y encontramos evidencia que respalda los mecanismos de crecimiento de la membrana.

Gage, F.H. Nuevas neuronas nacen en el cerebro humano adulto. (2025) Medicina Natural. DOI: 10.1038/s41591-025-03497-x

Gage, F.H. Neurogénesis adulta en el giro dentado humano. (2025) Hipocampo. 35(1):e23655. DOI: 10.1002/hipo.23655

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Rungratsameetaweemana, N., Kim, R., Chotibut, T., Sejnowski, T.J. El ruido aleatorio promueve dinámicas sinápticas lentas y heterogéneas importantes para el cálculo robusto de la memoria de trabajo. (2025) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 122(3):e2316745122. DOI: 10.1073/pnas.2316745122

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Cassidy, R.M., Macias, A.V., Lagos, W.N., Ugorji, C., Callaway, E.M. Organización complementaria de los circuitos cortico-tálamo-corticales del conductor y modulador del ratón. (2025) Diario de Neurociencia. DOI: 10.1523/JNEUROSCI.1167-24.2024

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Las proyecciones corticocorticales (CC) en el sistema visual facilitan el procesamiento jerárquico de la información sensorial. Además de las conexiones CC directas, las vías indirectas cortico-talámico-corticales (CTC) que pasan por el núcleo del pulvinar del tálamo pueden transmitir señales sensoriales y mediar en las interacciones corticales según las exigencias conductuales. Si bien el pulvinar se conecta ampliamente con toda la corteza visual, se desconoce si las vías transtalámicas unen todas las áreas corticales o si siguen reglas organizativas sistemáticas. Debido a que las neuronas del pulvinar de ratón que se proyectan a diferentes áreas están entremezcladas espacialmente, ha sido difícil caracterizar sus relaciones de entrada/salida utilizando métodos anatómicos tradicionales. Para determinar la organización de los circuitos CTC, cartografiamos las áreas visuales superiores (HVA) de ratones machos y hembras mediante imágenes de señales intrínsecas y seleccionamos cinco vías pulvinares→HVA para el rastreo de la rabia específico de la proyección. Alineamos el tejido cortical post mortem con los mapas in vivo para cuantificar con precisión las áreas y los tipos de células que se proyectan a cada población pulvinar→HVA. Las entradas "impulsoras" corticotalámicas de la capa 5 (L5CT) al pulvinar se originan predominantemente en la corteza visual primaria (V1), en consonancia con la jerarquía del CC. Las entradas L5CT de las HVA laterales evitan específicamente las conexiones recíprocas, lo que concuerda con la hipótesis de "ausencia de bucles fuertes". Por el contrario, las entradas "moduladoras" corticotalámicas de la capa 6 (L6CT) se distribuyen por todas las áreas y se inclinan hacia las conexiones recíprocas. A diferencia de estudios previos en primates, encontramos que cada HVA recibe una entrada disináptica del colículo superior. Los circuitos CTC en el pulvinar dependen, por lo tanto, tanto de la HVA de destino como del tipo de célula de entrada, de tal manera que las vías de orden superior impulsoras y moduladoras siguen reglas de conexión complementarias similares a las que rigen los circuitos CT de primer orden. Comprender la función del pulvinar requerirá el conocimiento de sus conexiones anatómicas. Utilizando técnicas de rastreo de la rabia de última generación, establecemos un mapa de las conexiones del pulvinar a nivel cerebral y de los circuitos CTC. Mientras que la vía tectopulvinaria de los primates se dirige selectivamente a las áreas visuales dorsales, encontramos una entrada del colículo superior (SC) ubicua en el ratón. Esta proyección extrageniculada sustenta el comportamiento inconsciente guiado visualmente, lo que sugiere que todas las áreas corticales visuales contribuyen a tales funciones en los ratones. Nuestros resultados también unifican hipótesis anatómicas de larga data. A saber, las entradas "impulsoras" del CTC son relés de alimentación directa y se ajustan a la hipótesis de "ausencia de bucles fuertes". Las entradas "moduladoras" del L6CT están sobrerrepresentadas en la HVA de destino, lo que refleja la conectividad recíproca descrita en estudios previos de rastreo masivo. En conjunto, estos hallazgos constituyen un mapa exhaustivo para orientar futuros estudios experimentales y teóricos sobre la función del pulvinar.

Pașca, S.P., Arlotta, P., Bateup, H.S., Camp, J.G., Cappello, S., Gage, F.H., Knoblich, J.A., Kriegstein, A.R., Lancaster, M.A., Ming, G.L., Novarino, G., Okano, H., Parmar, M., Park, I.H., Reiner, O., Song, H., Studer, L., Takahashi, J., Temple, S., Testa, G., Treutlein, B., Vaccarino, F.M., Vanderhaeghen, P., Young-Pearse, T. Un marco para estudios de organoides neuronales, ensambloides y trasplantes. (2024) Naturaleza. DOI: 10.1038/s41586-024-08487-6

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Praturu, A., Sharpee, T.O. Adaptive data embedding for curved spaces. (2024) iCiencia. 27(12):111266. DOI: 10.1016/j.isci.2024.111266

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Hirakis, S.P., Bartol, T.M., Autin, L., Amaro, R.E., Sejnowski, T.J. Electrophysical cardiac remodeling at the molecular level: insights into Ryanodine Receptor activation and calcium-induced calcium release from a stochastic explicit-particle model. (2024) Biophysical Journal. DOI: 10.1016/j.bpj.2024.09.029

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We present the first-ever, fully-discrete, stochastic model of triggered cardiac calcium dynamics. Using anatomically accurate subcellular cardiac myocyte geometries, we simulate the molecular players involved in calcium handling using high-resolution stochastic and explicit-particle methods at the level of an individual cardiac dyadic junction. Integrating data from multiple experimental sources, the model not only replicates the findings of traditional in silico studies and complements in vitro experimental data, but also reveals new insights into the molecular mechanisms driving cardiac dysfunction under stress and disease conditions. We improve upon older, non-discrete models using the same realistic geometry by incorporating molecular mechanisms for spontaneous, as well as triggered Calcium-Induced Calcium Release (CICR). Action potentials are used to activate L-type calcium channels (LTCCs), triggering CICR through Ryanodine receptors (RyR) on the surface of the sarcoplasmic reticulum. These improvements allow for the specific focus on the couplon: the structure-function relationship between LTCC and RyR. We investigate the electrophysical effects of normal and diseased action potentials on CICR and interrogate the effects of dyadic junction deformation through detubulation and orphaning of RyR. Our work demonstrates the importance of the electrophysical integrity of the CRU on CICR fidelity, giving insights into the molecular basis of heart disease. Finally, we provide a unique, detailed, molecular view of the CICR process using advanced rendering techniques. This easy-to-use model comes complete with tutorials and all necessary software for use and analysis so as to maximize usability and reproducibility. Our work focuses on quantifying, qualifying, and visualizing the behavior of the molecular species that underlie the function and dysfunction of subcellular cardiomyocyte systems.

Patel, D., Sejnowski, T., Siegelmann, H. Optimizing Attention and Cognitive Control Costs Using Temporally Layered Architectures. (2024) Computación neuronal.:1-30 DOI: 10.1162/neco_a_01718

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Son, G., Na, Y., Kim, Y., Son, J.H., Clemenson, G.D., Schafer, S.T., Yoo, J.Y., Parylak, S.L., Paquola, A., Do, H., Kim, D., Ahn, I., Ju, M., Kang, C.S., Ju, Y., Jung, E., McDonald, A.H., Park, Y., Kim, G., Paik, S.B., Hur, J., Kim, J., Han, Y.M., Lee, S.H., Gage, F.H., Kim, J.S., Han, J. El miR-124 coordina reguladores metabólicos que actúan en las etapas tempranas de la neurogénesis humana. (2024) Comunicaciones Biología. 7(1):1393. DOI: 10.1038/s42003-024-07089-2

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Barykina, N.V., Carey, E.M., Oliinyk, O.S., Nimmerjahn, A., Verkhusha, V.V. Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. (2024) Nature Communications. 15(1):7788. DOI: 10.1038/s41467-024-51857-x

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Muller, L., Churchland, P.S., Sejnowski, T.J. Transformers and cortical waves: encoders for pulling in context across time. (2024) Trends in Neurosciences. DOI: 10.1016/j.tins.2024.08.006

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Comstock, L., Carvalho, V.R., Lainscsek, C., Fallah, A., Sejnowski, T.J. Transcranial Magnetic Stimulation Facilitates Neural Speech Decoding. (2024) Brain Sci. 14(9). DOI: 10.3390/brainsci14090895

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Patiño, M., Rossa, M.A., Lagos, W.N., Patne, N.S., Callaway, E.M. Transcriptomic cell-type specificity of local cortical circuits. (2024) Neurona. DOI: 10.1016/j.neuron.2024.09.003

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Fernandes, S., Revanna, J., Pratt, J., Hayes, N., Marchetto, M.C., Gage, F.H. Modelado de la enfermedad de Alzheimer utilizando organoides cerebrales derivados de células humanas y modelos 3D. (2024) Fronteras en Neurociencia. 18:1434945. DOI: 10.3389/fnins.2024.1434945

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Minhas, P.S., Jones, J.R., Latif-Hernandez, A., Sugiura, Y., Durairaj, A.S., Wang, Q., Mhatre, S.D., Uenaka, T., Crapser, J., Conley, T., Ennerfelt, H., Jung, Y.J., Liu, L., Prasad, P., Jenkins, B.C., Ay, Y.A., Matrongolo, M., Goodman, R., Newmeyer, T., Heard, K., Kang, A., Wilson, E.N., Yang, T., Ullian, E.M., Serrano, G.E., Beach, T.G., Wernig, M., Rabinowitz, J.D., Suematsu, M., Longo, F.M., McReynolds, M.R., Gage, F.H., Andreasson, K.I. Restaurar el metabolismo de la glucosa en el hipocampo rescata la cognición a través de patologías de la enfermedad de Alzheimer. (2024) Ciencia. 385(6711):eabm6131. DOI: 10.1126/science.abm6131

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Alvarez-Kuglen, M., Ninomiya, K., Qin, H., Rodriguez, D., Fiengo, L., Farhy, C., Hsu, W.M., Kirk, B., Havas, A., Feng, G.S., Roberts, A.J., Anderson, R.M., Serrano, M., Adams, P.D., Sharpee, T.O., Terskikh, A.V. ImAge quantitates aging and rejuvenation. (2024) Nat Aging. DOI: 10.1038/s43587-024-00685-1

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Su, Y., Xu, J., Zhu, Z., Chin, J., Xu, L., Yu, H., Nudell, V., Dash, B., Moya, E.A., Ye, L., Nimmerjahn, A., Sun, X. Brainstem Dbh neurons control allergen-induced airway hyperreactivity. (2024) Naturaleza. DOI: 10.1038/s41586-024-07608-5

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Perez Garcia, G., Bicak, M., Buros, J., Haure-Mirande, J.V., Perez, G.M., Otero-Pagan, A., Gama Sosa, M.A., De Gasperi, R., Sano, M., Gage, F.H., Barlow, C., Dudley, J.T., Glicksberg, B.S., Wang, Y., Readhead, B., Ehrlich, M.E., Elder, G.A., Gandy, S. Efectos beneficiosos del ejercicio físico y un profármaco oralmente activo antagonista de mGluR2/3 sobre la neurogénesis y el comportamiento en un modelo de amiloidosis de Alzheimer. (2024) Frente Dement. 2:1198006. DOI: 10.3389/frdem.2023.1198006

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Carvalho, V.R., Mendes, E.M.A.M., Fallah, A., Sejnowski, T.J., Comstock, L., Lainscsek, C. Decoding imagined speech with delay differential analysis. (2024) Frontiers in Human Neuroscience. 18:1398065. DOI: 10.3389/fnhum.2024.1398065

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Schmidt, T.T., Tyer, C., Rughani, P., Haggblom, C., Jones, J.R., Dai, X., Frazer, K.A., Gage, F.H., Juul, S., Hickey, S., Karlseder, J. La secuenciación de telómeros de lectura larga de alta resolución revela mecanismos dinámicos en el envejecimiento y el cáncer. (2024) Nature Communications. 15(1):5149. DOI: 10.1038/s41467-024-48917-7

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Chen, Y., Zhang, H., Cameron, M., Sejnowski, T. Predictive sequence learning in the hippocampal formation. (2024) Neurona. DOI: 10.1016/j.neuron.2024.05.024

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Phang, H.J., Heimler, S.R., Scandalis, L.M., Wing, D., Moran, R., Nichols, J.F., Moreno, D., Shadel, G.S., Gage, F.H., Molina, A.J.A. Protocolo para la Cohorte Clínica del Centro Nathan Shock de San Diego: un nuevo recurso para estudios de envejecimiento humano. (2024) BMJ Open. 14(6):e082659. DOI: 10.1136/bmjopen-2023-082659

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Herdy, J.R., Mertens, J., Gage, F.H. La senescencia neuronal puede impulsar el envejecimiento cerebral. ENLACE AL TEXTO COMPLETO GRATUITO https://www.science.org/stoken/author-tokens/ST-1962/full [Pubmed] (2024) Ciencia. 384(6703):1404-1406. DOI: 10.1126/science.adi3450

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Minhas, P.S., Jones, J.R., Latif-Hernandez, A., Sugiura, Y., Durairaj, A.S., Uenaka, T., Wang, Q., Mhatre, S.D., Liu, L., Conley, T., Ennerfelt, H., Jung, Y.J., Prasad, P., Jenkins, B.C., Goodman, R., Newmeyer, T., Heard, K., Kang, A., Wilson, E.N., Ullian, E.M., Serrano, G.E., Beach, T.G., Rabinowitz, J.D., Wernig, M., Suematsu, M., Longo, F.M., McReynolds, M.R., Gage, F.H., Andreasson, K.I. Restaurar el metabolismo de la glucosa en el hipocampo rescata la cognición a través de patologías de la enfermedad de Alzheimer. (2024) bioRxiv. DOI: 10.1101/2024.06.23.598940

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El deterioro del metabolismo cerebral de la glucosa es una característica patológica de la enfermedad de Alzheimer (EA), y estudios proteómicos recientes ponen de relieve una alteración del metabolismo de los carbohidratos en las células gliales a medida que avanza la enfermedad. En este trabajo, informamos que la inhibición de la indolamina-2,3-dioxigenasa 1 (IDO1), que metaboliza el triptófano a kynurenina (KYN) en el primer paso de la vía de la kynurenina, rescata la función de memoria y la plasticidad del hipocampo en modelos preclínicos de patología amiloide y tau al restaurar el apoyo metabólico astrocítico de las neuronas. La activación de la IDO1 en los astrocitos por los oligómeros de beta-amiloide y tau, dos de los principales efectores patológicos de la EA, aumenta la KYN y suprime la glucólisis de manera dependiente del AhR. Por el contrario, la inhibición farmacológica de la IDO1 restaura la glucólisis y la producción de lactato. En ratones productores de amiloide y 5XFAD, así como en ratones P301S productores de tau, la inhibición de IDO1 restaura la memoria espacial y mejora el metabolismo de la glucosa en el hipocampo, según análisis metabolómicos y de MALDI-MS. El bloqueo de IDO1 también rescata la potenciación a largo plazo (LTP) del hipocampo de una manera dependiente del transportador de monocarboxilatos (MCT), lo que sugiere que la actividad de IDO1 altera el apoyo metabólico de los astrocitos a las neuronas. De hecho, el marcaje masivo in vitro de astrocitos humanos demuestra que IDO1 regula la generación de lactato por parte de los astrocitos, el cual es luego absorbido por las neuronas humanas. En cocultivos de astrocitos y neuronas derivadas de sujetos con EA, la transferencia deficiente de lactato de los astrocitos a las neuronas se corrigió mediante la inhibición de la IDO1, lo que dio lugar a una mejora del metabolismo de la glucosa neuronal. Por lo tanto, la actividad de la IDO1 altera el apoyo metabólico de los astrocitos a las neuronas tanto en las patologías amiloides como en las tau, y en un modelo de neuronas derivadas de iPSC de la EA. Estos hallazgos también sugieren que los inhibidores de la IDO1 desarrollados para la terapia complementaria en el cáncer podrían reutilizarse para el tratamiento de enfermedades neurodegenerativas mediadas por amiloide y tau.

Stern, S., Zhang, L., Wang, M., Wright, R., Rosh, I., Hussein, Y., Stern, T., Choudhary, A., Tripathi, U., Reed, P., Sadis, H., Nayak, R., Shemen, A., Agarwal, K., Cordeiro, D., Peles, D., Hang, Y., Mendes, A.P.D., Baul, T.D., Roth, J.G., Coorapati, S., Boks, M.P., McCombie, W.R., Hulshoff Pol, H., Brennand, K.J., Réthelyi, J.M., Kahn, R.S., Marchetto, M.C., Gage, F.H. Gemelos monocigóticos discordantes para esquizofrenia difieren en maduración y transmisión sináptica. (2024) Psiquiatría Molecular. DOI: 10.1038/s41380-024-02561-1

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La esquizofrenia afecta aproximadamente al 11 % de la población mundial. Se sabe que la genética, la epigenética y los factores ambientales influyen en este trastorno psiquiátrico. Si bien existe una alta concordancia en los gemelos monocigóticos, alrededor de la mitad de los pares de gemelos son discordantes en cuanto a la esquizofrenia. Para abordar la cuestión de cómo y cuándo se produce la concordancia en gemelos monocigóticos, hemos obtenido fibroblastos de dos pares de gemelos discordantes en esquizofrenia (un hermano con esquizofrenia mientras que el segundo no la padece) y tres pares de gemelos sanos (ambos hermanos están sanos). Hemos preparado modelos de iPSC para estos tres grupos de pacientes con esquizofrenia, gemelos no afectados y gemelos sanos. Cuando comenzó el estudio, los gemelos se consideraban sanos y no afectados, pero posteriormente se les diagnosticó a ambos un trastorno depresivo. Las iPSC reprogramadas se diferenciaron en neuronas del hipocampo para medir las anomalías neurofisiológicas en los pacientes. Descubrimos que las neuronas derivadas de los pacientes con esquizofrenia presentaban una menor arborización, eran hipoexcitables con características de picos inmaduros y mostraban una reducción significativa de la actividad sináptica con desregulación en los genes relacionados con las sinapsis. Curiosamente, las neuronas derivadas de los hermanos gemelos no afectados por la esquizofrenia formaron otro grupo distinto que se diferenciaba de las neuronas del grupo de los hermanos gemelos afectados, pero también de las neuronas del grupo de los gemelos de control. Es importante destacar que su actividad sináptica no se vio afectada. Nuestras mediciones, obtenidas de pacientes con esquizofrenia y sus gemelos monocigóticos y comparadas también con gemelos sanos de control, apuntan a los déficits sinápticos del hipocampo como un mecanismo central en la esquizofrenia.

Wu, Y., Temple, B.A., Sevilla, N., Zhang, J., Zhu, H., Zolotavin, P., Jin, Y., Duarte, D., Sanders, E., Azim, E., Nimmerjahn, A., Pfaff, S.L., Luan, L., Xie, C. Ultraflexible electrodes for recording neural activity in the mouse spinal cord during motor behavior. (2024) Cell Reports. 43(5):114199. DOI: 10.1016/j.celrep.2024.114199

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Tripathi, U., Rosh, I., Ben Ezer, R., Nayak, R., Hussein, Y., Choudhary, A., Djamus, J., Manole, A., Houlden, H., Gage, F.H., Stern, S. Genes de MEC regulados al alza y aumento de la actividad sináptica en neuronas dopaminérgicas humanas con mutaciones en PINK1/PRKN en la enfermedad de Parkinson. (2024) NPJ Parkinson's Dis. 10(1):103. DOI: 10.1038/s41531-024-00715-0

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La enfermedad de Parkinson (EP) es la segunda enfermedad neurodegenerativa más frecuente. Los síntomas primarios de la EP surgen con la pérdida de neuronas dopaminérgicas (DA) en la sustancia negra compacta, pero la EP también afecta al hipocampo y a la corteza cerebral, normalmente en sus etapas más avanzadas. Aproximadamente el 15 % de los casos de EP son hereditarios y se deben a una mutación genética. Dos de los genes más asociados con la EP familiar de inicio temprano autosómica recesiva (AR) son PINK1 y PRKN. Se necesitan estudios in vitro de estas mutaciones genéticas para comprender los cambios neurofisiológicos en las neuronas de los pacientes que pueden contribuir a la neurodegeneración. En este trabajo, generamos y diferenciamos neuronas dopaminérgicas y del hipocampo a partir de células madre pluripotentes inducidas humanas (hiPSC) derivadas de dos pacientes con una doble mutación en sus genes PINK1 y PRKN (uno homocigoto y otro heterocigoto) y evaluamos su neurofisiología en comparación con dos controles sanos. Demostramos que la actividad sináptica de las neuronas de la EP generadas a partir de pacientes con las mutaciones PINK1 y PRKN está alterada en el hipocampo y en las neuronas dopaminérgicas. Las neuronas dopaminérgicas mutantes presentaban una actividad postsináptica excitatoria aumentada. Además, las neuronas DA con la mutación homocigótica de PINK1 exhibieron diferencias electrofisiológicas más pronunciadas en comparación con las neuronas de control. El análisis de la red de señalización de los resultados de la secuenciación de ARN reveló que la adhesión focal y la vía del receptor de la MEC eran las dos vías más reguladas al alza en las neuronas de la EP mutantes. Nuestros hallazgos revelan que los fenotipos vinculados a las mutaciones de PINK1 y PRKN difieren de los de otras mutaciones de la EP, lo que sugiere una interacción única entre estas dos mutaciones que impulsa diferentes mecanismos de la EP.

Mi, X., Chen, A.B., Duarte, D., Carey, E., Taylor, C.R., Braaker, P.N., Bright, M., Almeida, R.G., Lim, J.X., Rutten, V.M., Zheng, W., Wang, M., Reitman, M.E., Wang, Y., Poskanzer, K.E., Lyons, D.A., Nimmerjahn, A., Ahrens, M.B., Yu, G. Fast, Accurate, and Versatile Data Analysis Platform for the Quantification of Molecular Spatiotemporal Signals. (2024) bioRxiv. DOI: 10.1101/2024.05.02.592259

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Zocher, S., McCloskey, A., Karasinsky, A., Schulte, R., Friedrich, U., Lesche, M., Rund, N., Gage, F.H., Hetzer, M.W., Toda, T. Persistencia de ARN nucleares a lo largo de la vida en el cerebro de ratón. (2024) Ciencia. 384(6691):53-59. DOI: 10.1126/science.adf3481

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Samavat, M., Bartol, T.M., Harris, K.M., Sejnowski, T.J. Synaptic Information Storage Capacity Measured With Information Theory. (2024) Computación neuronal. 36(5):781-802. DOI: 10.1162/neco_a_01659

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Husar, A., Ordyan, M., Garcia, G.C., Yancey, J.G., Saglam, A.S., Faeder, J.R., Bartol, T.M., Kennedy, M.B., Sejnowski, T.J. MCell4 with BioNetGen: A Monte Carlo simulator of rule-based reaction-diffusion systems with Python interface. (2024) PLOS Biología Computacional. 20(4):e1011800. DOI: 10.1371/journal.pcbi.1011800

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Adusei, M., Callaway, E.M., Usrey, W.M., Briggs, F. Parallel streams of direct corticogeniculate feedback from mid-level extrastriate cortex in the macaque monkey. (2024) eNeuro. DOI: 10.1523/ENEURO.0364-23.2024

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First order thalamic nuclei receive feedforward signals from peripheral receptors and relay these signals to primary sensory cortex. Primary sensory cortex, in turn, provides reciprocal feedback to first order thalamus. Because the vast majority of sensory thalamocortical inputs target primary sensory cortex, their complementary corticothalamic neurons are assumed to be similarly restricted to primary sensory cortex. We upend this assumption by characterizing morphologically diverse neurons in multiple mid-level visual cortical areas of the primate () brain that provide direct feedback to the primary visual thalamus, the dorsal lateral geniculate nucleus (LGN). Although the majority of geniculocortical neurons project to primary visual cortex (V1), a minority, located mainly in the koniocellular LGN layers, provide direct input to extrastriate visual cortex. These "V1-bypassing" projections may be implicated in blindsight. We hypothesized that geniculocortical inputs directly targeting extrastriate cortex should be complemented by reciprocal corticogeniculate circuits. Using virus-mediated circuit tracing, we discovered corticogeniculate neurons throughout three mid-level extrastriate areas: MT, MST, and V4. Quantitative morphological analyses revealed non-uniform distributions of unique cell types across areas. Many extrastriate corticogeniculate neurons had spiny stellate morphology, suggesting possible targeting of koniocellular LGN layers. Importantly though, multiple morphological types were observed across areas. Such morphological diversity could suggest parallel streams of V1-bypassing corticogeniculate feedback at multiple stages of the visual processing hierarchy. Furthermore, the presence of corticogeniculate neurons across visual cortex necessitates a reevaluation of the LGN as a hub for visual information rather than a simple relay. First order thalamic nuclei are most strongly connected in feedforward and feedback directions with primary sensory cortex. In the visual system, direct feedforward circuits link the visual thalamus with higher-order, extrastriate visual areas, although the function of these less-common pathways remains unknown. Here we demonstrate for the first time the presence of complementary direct feedback connections between multiple extrastriate visual cortical areas and the visual thalamus in the primate brain. Although sparse, extrastriate corticogeniculate circuits are consistently present and contain morphologically diverse neurons, suggesting parallel streams of extrastriate feedback. These findings indicate an evolutionary conservation of circuits that integrate information from throughout the visual processing hierarchy within the visual thalamus.

Podvin, S., Jones, J., Kang, A., Goodman, R., Reed, P., Lietz, C.B., Then, J., Lee, K.C., Eyler, L.T., Jeste, D.V., Gage, F.H., Hook, V. El modelo neuronal humano de esquizofrenia muestra desregulación de la cromogranina B y firmas de transmisores de neuropéptidos relacionados. (2024) Psiquiatría Molecular. DOI: 10.1038/s41380-024-02422-x

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La esquizofrenia (SZ) es una enfermedad mental grave y un trastorno neuropsiquiátrico del cerebro cuyos síntomas conductuales incluyen alucinaciones, delirios, comportamiento desorganizado y deterioro cognitivo. La regulación de tales comportamientos requiere la utilización de neurotransmisores liberados para mediar la comunicación entre células, los cuales son esenciales para las funciones cerebrales tanto en condiciones de salud como de enfermedad. Planteamos la hipótesis de que la SZ podría implicar una desregulación de los neurotransmisores secretados por las neuronas. Para comprender mejor la SZ en humanos, se obtuvieron neuronas inducidas (iN) de pacientes con SZ y de sujetos de control sanos con el fin de investigar los neurotransmisores peptídicos, conocidos como neuropéptidos, que representan la clase principal de transmisores. Las iN fueron sometidas a despolarización mediante altas concentraciones de KCl en el medio de cultivo, y los neuropéptidos secretados fueron identificados y cuantificados mediante espectrometría de masas en tándem nano-LC-MS/MS. Se identificaron varios neuropéptidos en las neuronas derivadas de pacientes con esquizofrenia, incluyendo la cromogranina B (CHGB), la neurotensina y el péptido natriurético. Centrándose en los principales neuropéptidos CHGB secretados, los resultados revelaron diferencias en las iN de SZ en comparación con las neuronas iN de control. Se encontró un menor número de péptidos CHGB distintos en los medios de secreción de SZ en comparación con los controles. El mapeo de los péptidos al precursor de CHGB reveló péptidos exclusivos de SZ o de control, y péptidos comunes a ambas condiciones. Además, las iN secretaron neuropéptidos tanto en condiciones de KCl como basales (sin KCl). Estos hallazgos concuerdan con los informes que indican que los niveles de cromogranina B están reducidos en el líquido cefalorraquídeo y en regiones específicas del cerebro de pacientes con esquizofrenia. Estos hallazgos sugieren que las iN derivadas de pacientes con esquizofrenia pueden modelar la disminución de los neuropéptidos de CHGB observada en la esquizofrenia humana.

Toda, T., Bedrosian, T.A., Schafer, S.T., Cuoco, M.S., Linker, S.B., Ghassemzadeh, S., Mitchell, L., Whiteley, J.T., Novaresi, N., McDonald, A.H., Gallina, I.S., Yoon, H., Hester, M.E., Pena, M., Lim, C., Suljic, E., AlFatah Mansour, A., Boulard, M., Parylak, S.L., Gage, F.H. Los elementos nucleares largos intercalados protegen a los progenitores neuronales de la diferenciación precoz. (2024) Cell Reports. 43(2):113774. DOI: 10.1016/j.celrep.2024.113774

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Bartol, T.M., Ordyan, M., Sejnowski, T.J., Rangamani, P., Kennedy, M.B. A spatial model of autophosphorylation of CaMKII in a glutamatergic spine suggests a network-driven kinetic mechanism for bistable changes in synaptic strength. (2024) bioRxiv. DOI: 10.1101/2024.02.02.578696

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Rosh, I., Tripathi, U., Hussein, Y., Rike, W.A., Djamus, J., Shklyar, B., Manole, A., Houlden, H., Winkler, J., Gage, F.H., Stern, S. Disfunción sináptica y desregulación de la matriz extracelular en neuronas dopaminérgicas de pacientes con enfermedad de Parkinson esporádica y con mutación E326K en el gen GBA1. (2024) NPJ Parkinson's Dis. 10(1):38. DOI: 10.1038/s41531-024-00653-x

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Wang, H., Dey, O., Lagos, W.N., Behnam, N., Callaway, E.M., Stafford, B.K. Parallel pathways carrying direction-and orientation-selective retinal signals to layer 4 of the mouse visual cortex. (2024) Cell Reports. 43(3):113830. DOI: 10.1016/j.celrep.2024.113830

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Miao, S., Fourgeaud, L., Burrola, P.G., Stern, S., Zhang, Y., Happonen, K.E., Novak, S.W., Gage, F.H., Lemke, G. Tyro3 promueve la maduración de las sinapsis glutamatérgicas. (2024) Fronteras en Neurociencia. 18:1327423. DOI: 10.3389/fnins.2024.1327423

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Wang, M., Zhang, L., Novak, S.W., Yu, J., Gallina, I.S., Xu, L.L., Lim, C.K., Fernandes, S., Shokhirev, M.N., Williams, A.E., Saxena, M.D., Coorapati, S., Parylak, S.L., Quintero, C., Molina, E., Andrade, L.R., Manor, U., Gage, F.H. Diversificación morfológica y maduración funcional de astrocitos humanos en organoides corticales enriquecidos con glía trasplantados en cerebros de ratón. (2024) Nature Biotechnology. DOI: 10.1038/s41587-024-02157-8

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Sameni, S., Bartol, T.M., Corey-Bloom, J., Sejnowski, T.J. Computer simulations predict the impact of neuronal atrophy on the calcium dynamics in Huntington's disease. (2024) PNAS Nexus. 3(1):pgad443. DOI: 10.1093/pnasnexus/pgad443

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Samavat, M., Bartol, T.M., Bromer, C., Hubbard, D.D., Hanka, D.C., Kuwajima, M., Mendenhall, J.M., Parker, P.H., Bowden, J.B., Abraham, W.C., Sejnowski, T.J., Harris, K.M. Long-Term Potentiation Produces a Sustained Expansion of Synaptic Information Storage Capacity in Adult Rat Hippocampus. (2024) bioRxiv. DOI: 10.1101/2024.01.12.574766

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Zhang, L.A., Li, P., Callaway, E.M. High-Resolution Laminar Identification in Macaque Primary Visual Cortex Using Neuropixels Probes. (2024) bioRxiv. DOI: 10.1101/2024.01.23.576944

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Zhou, J., Zhang, Z., Wu, M., Liu, H., Pang, Y., Bartlett, A., Peng, Z., Ding, W., Rivkin, A., Lagos, W.N., Williams, E., Lee, C.T., Miyazaki, P.A., Aldridge, A., Zeng, Q., Salinda, J.L.A., Claffey, N., Liem, M., Fitzpatrick, C., Boggeman, L., Yao, Z., Smith, K.A., Tasic, B., Altshul, J., Kenworthy, M.A., Valadon, C., Nery, J.R., Castanon, R.G., Patne, N.S., Vu, M., Rashid, M., Jacobs, M., Ito, T., Osteen, J., Emerson, N., Lee, J., Cho, S., Rink, J., Huang, H.H., Pinto-Duartec, A., Dominguez, B., Smith, J.B., O'Connor, C., Zeng, H., Chen, S., Lee, K.F., Mukamel, E.A., Jin, X., Margarita Behrens, M., Ecker, J.R., Callaway, E.M. Brain-wide correspondence of neuronal epigenomics and distant projections. (2023) Naturaleza. 624(7991):355-365. DOI: 10.1038/s41586-023-06823-w

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Zemke, N.R., Armand, E.J., Wang, W., Lee, S., Zhou, J., Li, Y.E., Liu, H., Tian, W., Nery, J.R., Castanon, R.G., Bartlett, A., Osteen, J.K., Li, D., Zhuo, X., Xu, V., Chang, L., Dong, K., Indralingam, H.S., Rink, J.A., Xie, Y., Miller, M., Krienen, F.M., Zhang, Q., Taskin, N., Ting, J., Feng, G., McCarroll, S.A., Callaway, E.M., Wang, T., Lein, E.S., Behrens, M.M., Ecker, J.R., Ren, B. Conserved and divergent gene regulatory programs of the mammalian neocortex. (2023) Naturaleza. 624(7991):390-402. DOI: 10.1038/s41586-023-06819-6

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Patiño, M., Lagos, W.N., Patne, N.S., Miyazaki, P.A., Bhamidipati, S.K., Collman, F., Callaway, E.M. Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. (2023) Elife. 12. DOI: 10.7554/eLife.89297

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Chung, B., Zia, M., Thomas, K.A., Michaels, J.A., Jacob, A., Pack, A., Williams, M.J., Nagapudi, K., Teng, L.H., Arrambide, E., Ouellette, L., Oey, N., Gibbs, R., Anschutz, P., Lu, J., Wu, Y., Kashefi, M., Oya, T., Kersten, R., Mosberger, A.C., O'Connell, S., Wang, R., Marques, H., Mendes, A.R., Lenschow, C., Kondakath, G., Kim, J.J., Olson, W., Quinn, K.N., Perkins, P., Gatto, G., Thanawalla, A., Coltman, S., Kim, T., Smith, T., Binder-Markey, B., Zaback, M., Thompson, C.K., Giszter, S., Person, A., Goulding, M., Azim, E., Thakor, N., O'Connor, D., Trimmer, B., Lima, S.Q., Carey, M.R., Pandarinath, C., Costa, R.M., Pruszynski, J.A., Bakir, M., Sober, S.J. Myomatrix arrays for high-definition muscle recording. (2023) Elife. 12. DOI: 10.7554/eLife.88551

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Lainscsek, C., Salami, P., Carvalho, V.R., Mendes, E.M.A.M., Fan, M., Cash, S.S., Sejnowski, T.J. Network-motif delay differential analysis of brain activity during seizures. (2023) Chaos. 33(12). DOI: 10.1063/5.0165904

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Garrison, M.A., Jang, Y., Bae, T., Cherskov, A., Emery, S.B., Fasching, L., Jones, A., Moldovan, J.B., Molitor, C., Pochareddy, S., Peters, M.A., Shin, J.H., Wang, Y., Yang, X., Akbarian, S., Chess, A., Gage, F.H., Gleeson, J.G., Kidd, J.M., McConnell, M., Mills, R.E., Moran, J.V., Park, P.J., Sestan, N., Urban, A.E., Vaccarino, F.M., Walsh, C.A., Weinberger, D.R., Wheelan, S.J., Abyzov, A. Recursos de datos genómicos de la Red de Mosaicismo Somático Cerebral para enfermedades neuropsiquiátricas. (2023) Datos científicos. 10(1):813. DOI: 10.1038/s41597-023-02645-7

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Tsuda, B., Richmond, B.J., Sejnowski, T.J. Exploring strategy differences between humans and monkeys with recurrent neural networks. (2023) PLOS Biología Computacional. 19(11):e1011618. DOI: 10.1371/journal.pcbi.1011618

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Manole, A., Wong, T., Rhee, A., Novak, S., Chin, S.M., Tsimring, K., Paucar, A., Williams, A., Newmeyer, T.F., Schafer, S.T., Rosh, I., Kaushik, S., Hoffman, R., Chen, S., Wang, G., Snyder, M., Cuervo, A.M., Andrade, L., Manor, U., Lee, K., Jones, J.R., Stern, S., Marchetto, M.C., Gage, F.H. Las mutaciones de NGLY1 causan agregación de proteínas en neuronas humanas. (2023) Cell Reports. 42(12):113466. DOI: 10.1016/j.celrep.2023.113466

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Alvarez-Kuglen, M., Rodriguez, D., Qin, H., Ninomiya, K., Fiengo, L., Farhy, C., Hsu, W.M., Havas, A., Feng, G.S., Roberts, A.J., Anderson, R.M., Serrano, M., Adams, P.D., Sharpee, T.O., Terskikh, A.V. Imaging-based chromatin and epigenetic age, ImAge, quantitates aging and rejuvenation. (2023) Res. Cuadrado. DOI: 10.21203/rs.3.rs-3479973/v1

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Jorstad, N.L., Close, J., Johansen, N., Yanny, A.M., Barkan, E.R., Travaglini, K.J., Bertagnolli, D., Campos, J., Casper, T., Crichton, K., Dee, N., Ding, S.L., Gelfand, E., Goldy, J., Hirschstein, D., Kiick, K., Kroll, M., Kunst, M., Lathia, K., Long, B., Martin, N., McMillen, D., Pham, T., Rimorin, C., Ruiz, A., Shapovalova, N., Shehata, S., Siletti, K., Somasundaram, S., Sulc, J., Tieu, M., Torkelson, A., Tung, H., Callaway, E.M., Hof, P.R., Keene, C.D., Levi, B.P., Linnarsson, S., Mitra, P.P., Smith, K., Hodge, R.D., Bakken, T.E., Lein, E.S. Transcriptomic cytoarchitecture reveals principles of human neocortex organization. (2023) Ciencia. 382(6667):eadf6812. DOI: 10.1126/science.adf6812

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Lainscsek, C., Mendes, E.M.A.M., Salgado, G.H.O., Sejnowski, T.J. Transformations that preserve the uniqueness of the differential form for Lorenz-like systems. (2023) Chaos. 33(10). DOI: 10.1063/5.0156237

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Klug, J.R., Yan, X., Hoffman, H.A., Engelhardt, M.D., Osakada, F., Callaway, E.M., Jin, X. Las proyecciones corticales asimétricas a las vías directa e indirecta del cuerpo estriado controlan las acciones de forma distinta. (2023) bioRxiv. DOI: 10.1101/2023.10.02.560589

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Daboussi, L., Costaguta, G., Gullo, M., Jasinski, N., Pessino, V., O'Leary, B., Lettieri, K., Driscoll, S., Pfaff, S.L. Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair. (2023) Cell Reports. 42(11):113282. DOI: 10.1016/j.celrep.2023.113282

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Garcia, G.C., Gupta, K., Bartol, T.M., Sejnowski, T.J., Rangamani, P. Mitochondrial morphology governs ATP production rate. (2023) J Gen Physiol. 155(9). DOI: 10.1085/jgp.202213263

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Globig, A.M., Zhao, S., Roginsky, J., Maltez, V.I., Guiza, J., Avina-Ochoa, N., Heeg, M., Araujo Hoffmann, F., Chaudhary, O., Wang, J., Senturk, G., Chen, D., O'Connor, C., Pfaff, S., Germain, R.N., Schalper, K.A., Emu, B., Kaech, S.M. The β-adrenergic receptor links sympathetic nerves to T cell exhaustion. (2023) Naturaleza. DOI: 10.1038/s41586-023-06568-6

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Li, J.S., Sarma, A.A., Sejnowski, T.J., Doyle, J.C. Internal feedback in the cortical perception-action loop enables fast and accurate behavior. (2023) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 120(39):e2300445120. DOI: 10.1073/pnas.2300445120

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Wang, H., Dey, O., Lagos, W.N., Behnam, N., Callaway, E.M., Stafford, B.K. Parallel pathways carrying direction and orientation selective retinal signals to layer 4 of mouse visual cortex. (2023) bioRxiv. DOI: 10.1101/2023.09.18.558281

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Mizrahi, L., Choudhary, A., Ofer, P., Goldberg, G., Milanesi, E., Kelsoe, J.R., Gurwitz, D., Alda, M., Gage, F.H., Stern, S. Los genes de inmunoglobulina expresados en líneas celulares de linfoblastoides discernen y predicen la respuesta a litio en pacientes con trastorno bipolar. (2023) Psiquiatría Molecular. DOI: 10.1038/s41380-023-02183-z

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Traxler, L., Lucciola, R., Herdy, J.R., Jones, J.R., Mertens, J., Gage, F.H. Cambios en el estado de las células neuronales y pérdida de su destino en el envejecimiento y enfermedades relacionadas con la edad. (2023) Nat Rev Neurol. DOI: 10.1038/s41582-023-00815-0

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Schafer, S.T., Mansour, A.A., Schlachetzki, J.C.M., Pena, M., Ghassemzadeh, S., Mitchell, L., Mar, A., Quang, D., Stumpf, S., Ortiz, I.S., Lana, A.J., Baek, C., Zaghal, R., Glass, C.K., Nimmerjahn, A., Gage, F.H. Un modelo de organoide neuroinmune in vivo para estudiar fenotipos de microglía humana. (2023) Móvil. 186(10):2111-2126.e20. DOI: 10.1016/j.cell.2023.04.022

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Arlotta, P., Gage, F.H. Organoides neuronales y la búsqueda para comprender y tratar enfermedades psiquiátricas. (2023) Psiquiatría Biológica. 93(7):588-589. DOI: 10.1016/j.biopsych.2023.01.021

Zemke, N.R., Armand, E.J., Wang, W., Lee, S., Zhou, J., Li, Y.E., Liu, H., Tian, W., Nery, J.R., Castanon, R.G., Bartlett, A., Osteen, J.K., Li, D., Zhuo, X., Xu, V., Miller, M., Krienen, F.M., Zhang, Q., Taskin, N., Ting, J., Feng, G., McCarroll, S.A., Callaway, E.M., Wang, T., Behrens, M.M., Lein, E.S., Ecker, J.R., Ren, B. Comparative single cell epigenomic analysis of gene regulatory programs in the rodent and primate neocortex. (2023) bioRxiv. DOI: 10.1101/2023.04.08.536119

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Kobayashi, Y., Shigyo, M., Platoshyn, O., Marsala, S., Kato, T., Takamura, N., Yoshida, K., Kishino, A., Bravo-Hernandez, M., Juhas, S., Juhasova, J., Studenovska, H., Proks, V., Driscoll, S.P., Glenn, T.D., Pfaff, S.L., Ciacci, J.D., Marsala, M. Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: Post-Grafting Safety Characterization in Rats and Adult Pig. (2023) Cell Transplantation. 32:9636897221107009. DOI: 10.1177/09636897221107009

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Mishra, H.K., Wei, H., Rohr, K.E., Ko, I., Nievergelt, C.M., Maihofer, A.X., Shilling, P.D., Alda, M., Berrettini, W.H., Brennand, K.J., Calabrese, J.R., Coryell, W.H., Frye, M., Gage, F., Gershon, E., McInnis, M.G., Nurnberger, J., Oedegaard, K.J., Zandi, P.P., Kelsoe, J.R., McCarthy, M.J. Contribuciones de los genes del reloj circadiano a la supervivencia celular en modelos de fibroblastos de trastorno bipolar sensible al litio. (2023) Eur Neuropsychopharmacol. 74:1-14. DOI: 10.1016/j.euroneuro.2023.04.009

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Shekhtmeyster, P., Duarte, D., Carey, E.M., Ngo, A., Gao, G., Olmstead, J.A., Nelson, N.A., Nimmerjahn, A. Trans-segmental imaging in the spinal cord of behaving mice. (2023) Nature Biotechnology. DOI: 10.1038/s41587-023-01700-3

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Kempermann, G., Song, H., Gage, F.H. Neurogénesis adulta en el hipocampo. (2023) Hipocampo. DOI: 10.1002/hipo.23525

Shekhtmeyster, P., Carey, E.M., Duarte, D., Ngo, A., Gao, G., Nelson, N.A., Clark, C.L., Nimmerjahn, A. Multiplex translaminar imaging in the spinal cord of behaving mice. (2023) Nature Communications. 14(1):1427. DOI: 10.1038/s41467-023-36959-2

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Zador, A., Escola, S., Richards, B., Ölveczky, B., Bengio, Y., Boahen, K., Botvinick, M., Chklovskii, D., Churchland, A., Clopath, C., DiCarlo, J., Ganguli, S., Hawkins, J., Körding, K., Koulakov, A., LeCun, Y., Lillicrap, T., Marblestone, A., Olshausen, B., Pouget, A., Savin, C., Sejnowski, T., Simoncelli, E., Solla, S., Sussillo, D., Tolias, A.S., Tsao, D. Catalyzing next-generation Artificial Intelligence through NeuroAI. (2023) Nature Communications. 14(1):1597. DOI: 10.1038/s41467-023-37180-x

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Niemsiri, V., Rosenthal, S.B., Nievergelt, C.M., Maihofer, A.X., Marchetto, M.C., Santos, R., Shekhtman, T., Alliey-Rodriguez, N., Anand, A., Balaraman, Y., Berrettini, W.H., Bertram, H., Burdick, K.E., Calabrese, J.R., Calkin, C.V., Conroy, C., Coryell, W.H., DeModena, A., Eyler, L.T., Feeder, S., Fisher, C., Frazier, N., Frye, M.A., Gao, K., Garnham, J., Gershon, E.S., Goes, F.S., Goto, T., Harrington, G.J., Jakobsen, P., Kamali, M., Kelly, M., Leckband, S.G., Lohoff, F.W., McCarthy, M.J., McInnis, M.G., Craig, D., Millett, C.E., Mondimore, F., Morken, G., Nurnberger, J.I., Donovan, C.O., Øedegaard, K.J., Ryan, K., Schinagle, M., Shilling, P.D., Slaney, C., Stapp, E.K., Stautland, A., Tarwater, B., Zandi, P.P., Alda, M., Fisch, K.M., Gage, F.H., Kelsoe, J.R. La adhesión focal se asocia con la respuesta al litio en el trastorno bipolar: evidencia de un análisis multi-ómico basado en redes. (2023) Psiquiatría Molecular. DOI: 10.1038/s41380-022-01909-9

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Little, J.C., Kaaronen, R.O., Hukkinen, J.I., Xiao, S., Sharpee, T., Farid, A.M., Nilchiani, R., Barton, C.M. Earth Systems to Anthropocene Systems: An Evolutionary, System-of-Systems, Convergence Paradigm for Interdependent Societal Challenges. (2023) Environ Sci Technol. DOI: 10.1021/acs.est.2c06203

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Ren, X., Liu, S., Virlogeux, A., Kang, S.J., Brusch, J., Liu, Y., Dymecki, S.M., Han, S., Goulding, M., Acton, D. Identification of an essential spinoparabrachial pathway for mechanical itch. (2023) Neurona. DOI: 10.1016/j.neuron.2023.03.013

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Hayashi, M., Gullo, M., Senturk, G., Di Costanzo, S., Nagasaki, S.C., Kageyama, R., Imayoshi, I., Goulding, M., Pfaff, S.L., Gatto, G. A spinal synergy of excitatory and inhibitory neurons coordinates ipsilateral body movements. (2023) bioRxiv. DOI: 10.1101/2023.03.21.533603

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Sejnowski, T.J. Large Language Models and the Reverse Turing Test. (2023) Computación neuronal.:1-34 DOI: 10.1162/neco_a_01563

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Parylak, S.L., Qiu, F., Linker, S.B., Gallina, I.S., Lim, C.K., Preciado, D., McDonald, A.H., Zhou, X., Gage, F.H. La transcripción relacionada con la actividad neuronal se ve atenuada en las células granulares del giro dentado inmaduras en comparación con las maduras. (2023) Hipocampo. DOI: 10.1002/hipo.23515

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Yang, J.Y., O'Connell, T.F., Hsu, W.M., Bauer, M.S., Dylla, K.V., Sharpee, T.O., Hong, E.J. Restructuring of olfactory representations in the fly brain around odor relationships in natural sources. (2023) bioRxiv. DOI: 10.1101/2023.02.15.528627

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Bermperidis, T., Schafer, S., Gage, F.H., Sejnowski, T., Torres, E.B. Interrogación Dinámica de Trayectorias Transcriptómicas Estocásticas Usando Genes Asociados a Enfermedades Revela Orígenes Distintos de Trastornos Neurológicos y Psiquiátricos. (2023) Fronteras en Neurociencia. 16:884707. DOI: 10.3389/fnins.2022.884707

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La llegada del acceso abierto a los datos genómicos ofrece nuevas oportunidades para retomar antiguos debates clínicos y abordarlos desde una perspectiva diferente. Reexaminamos la cuestión de si los trastornos psiquiátricos y neurológicos son distintos entre sí, evaluando el conjunto de genes asociados a los trastornos que se consideran psiquiátricos o neurológicos. Lo hacemos en el contexto de los datos del transcriptoma registrados a medida que las células madre embrionarias humanas se diferencian y se convierten en neuronas. Partiendo de capas probabilísticas de complejidad creciente, describimos la dinámica y las trayectorias estocásticas del transcriptoma completo y de los genes integrados asociados con trastornos psiquiátricos y/o neurológicos. Desde distribuciones marginales de la expresión de un gen en cientos de células, pasando por interacciones conjuntas consideradas globalmente para determinar el grado de dependencia por pares, hasta redes derivadas de grafos probabilísticos a lo largo de árboles de expansión máxima, hemos descubierto dos clases de genes fundamentalmente diferentes que subyacen a estos trastornos y los diferencian. Una clase de genes presenta una mayor variabilidad en la expresión y menores dependencias (genes de alta variabilidad de expresión, HEV); la otra tiene menor variabilidad y mayores dependencias (genes de baja variabilidad de expresión, LEV). Dan lugar a diferentes arquitecturas de red y diferentes estados de transición. Los genes HEV tienen grandes nodos centrales y una topología frágil, mientras que los genes LEV muestran un código más distribuido durante la maduración hacia el estado neuronal. Los genes LEV potencian la diferenciación entre trastornos psiquiátricos y neurológicos también a nivel de tejido en el cerebro, la médula espinal y las glándulas. Estos genes, con su baja variabilidad y sus estados ON/OFF asincrónicos que han sido tratados como datos brutos y excluidos de los análisis tradicionales, nos están ayudando a resolver esta vieja controversia en más de un nivel de investigación.

Fischer, K.B., Collins, H.K., Pang, Y., Roy, D.S., Zhang, Y., Feng, G., Li, S.J., Kepecs, A., Callaway, E.M. Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing. (2023) Journal of Comparative Neurology. DOI: 10.1002/cne.25451

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Perez-Garcia, G., Bicak, M., Haure-Mirande, J.V., Perez, G.M., Otero-Pagan, A., Gama Sosa, M.A., De Gasperi, R., Sano, M., Barlow, C., Gage, F.H., Readhead, B., Ehrlich, M.E., Gandy, S., Elder, G.A. BCI-838, un profármaco oralmente activo antagonista del receptor mGluR2/3, rescata déficits de comportamiento de aprendizaje en el modelo de ratón PS19 MAPT de tauopatía. (2023) Neurosci Lett.:137080 DOI: 10.1016/j.neulet.2023.137080

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Las tauopatías constituyen un grupo heterogéneo de trastornos neurodegenerativos que se distinguen clínica y patológicamente de la enfermedad de Alzheimer (EA), y cuya característica neuropatológica más destacada son las inclusiones de tau en las neuronas y/o la glía. El BCI-838 (MGS00210) es un profármaco antagonista de los receptores metabotrópicos de glutamato del grupo II (mGluR2/3). Anteriormente, informamos que el BCI-838 administrado por vía oral mejoró el comportamiento de aprendizaje y redujo la ansiedad en ratones transgénicos holandeses (APP), un modelo de la acumulación patológica de oligómeros de Aβ que se encuentra en la EA. En este estudio, investigamos los efectos del BCI-838 en ratones machos PS19 que expresan la mutación MAPT de la taupatía asociada con la degeneración lobular frontotemporal (DLFT) humana. Estos ratones desarrollan una taupatía relacionada con el envejecimiento sin acumulación de amiloide. Los ratones se dividieron en tres grupos experimentales: (1) ratones de tipo salvaje no transgénicos tratados con vehículo, (2) ratones PS19 tratados con vehículo y (3) ratones PS19 tratados con 5 mg/kg de BCI-838. Se utilizaron grupos de 10 a 13 ratones. El vehículo o el BCI-838 se administraron por sonda oral durante 4 semanas. Tras la administración del fármaco, se realizaron pruebas de comportamiento que consistían en una tarea de reconocimiento de objetos nuevos. Se llevaron a cabo dos estudios en los que se inició el tratamiento de los ratones a los 3 o 7 meses de edad. Un mes de tratamiento con BCI-838 revirtió los déficits en la memoria de reconocimiento en ratones PS19, independientemente de si el tratamiento se inició a los 3 o a los 7 meses de edad. Estos estudios amplían la utilidad potencial del BCI-838 a las afecciones neurodegenerativas que tienen la taupatía como base subyacente. También sugieren un mecanismo dependiente de mGluR2/3 como base de los déficits conductuales en ratones PS19.

Wang, M., Gage, F.H., Schafer, S.T. Estrategias de trasplante para mejorar la madurez y la complejidad celular en organoides cerebrales. (2023) Psiquiatría Biológica. DOI: 10.1016/j.biopsych.2023.01.004

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Herdy, J.R., Traxler, L., Agarwal, R.K., Karbacher, L., Schlachetzki, J.C.M., Boehnke, L., Zangwill, D., Galasko, D., Glass, C.K., Mertens, J., Gage, F.H. El aumento de la senescencia postmitótica en neuronas humanas envejecidas es una característica patológica de la enfermedad de Alzheimer. (2022) Células Madre. 29(12):1637-1652.e6. DOI: 10.1016/j.stem.2022.11.010

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Fu, C., Ngo, J., Zhang, S., Lu, L., Miron, A., Schafer, S., Gage, F.H., Jin, F., Schumacher, F.R., Wynshaw-Boris, A. Análisis correlativo novedoso identifica múltiples variaciones genómicas que impactan el TEA con macrocefalia. (2022) Genética Molecular Humana. DOI: 10.1093/hmg/ddac300

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Ronzano, R., Skarlatou, S., Barriga, B.K., Bannatyne, B.A., Bhumbra, G.S., Foster, J.D., Moore, J.D., Lancelin, C., Pocratsky, A.M., Özyurt, M.G., Smith, C.C., Todd, A.J., Maxwell, D.J., Murray, A.J., Pfaff, S.L., Brownstone, R.M., Zampieri, N., Beato, M. Spinal premotor interneurons controlling antagonistic muscles are spatially intermingled. (2022) Elife. 11. DOI: 10.7554/eLife.81976

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Wilson, M.N., Thunemann, M., Liu, X., Lu, Y., Puppo, F., Adams, J.W., Kim, J.H., Ramezani, M., Pizzo, D.P., Djurovic, S., Andreassen, O.A., Mansour, A.A., Gage, F.H., Muotri, A.R., Devor, A., Kuzum, D. Monitoreo multimodal de organoides corticales humanos implantados en ratones revela conexión funcional con la corteza visual. (2022) Nature Communications. 13(1):7945. DOI: 10.1038/s41467-022-35536-3

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Zhang, H., Rich, P.D., Lee, A.K., Sharpee, T.O. Hippocampal spatial representations exhibit a hyperbolic geometry that expands with experience. (2022) Nature Neuroscience. DOI: 10.1038/s41593-022-01212-4

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Rungratsameetaweemana, N., Lainscsek, C., Cash, S.S., Garcia, J.O., Sejnowski, T.J., Bansal, K. Brain network dynamics codify heterogeneity in seizure evolution. (2022) Brain Commun. 4(5):fcac234. DOI: 10.1093/braincomms/fcac234

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Dynamic functional brain connectivity facilitates adaptive cognition and behaviour. Abnormal alterations within such connectivity could result in disrupted functions observed across various neurological conditions. As one of the most common neurological disorders, epilepsy is defined by the seemingly random occurrence of spontaneous seizures. A central but unresolved question concerns the mechanisms by which extraordinarily diverse propagation dynamics of seizures emerge. Here, we applied a graph-theoretical approach to assess dynamic reconfigurations in the functional brain connectivity before, during and after seizures that display heterogeneous propagation patterns despite sharing similar cortical onsets. We computed time-varying functional brain connectivity networks from human intracranial recordings of 67 seizures (across 14 patients) that had a focal origin-49 of these focal seizures remained focal and 18 underwent a bilateral spread (focal to bilateral tonic-clonic seizures). We utilized functional connectivity networks estimated from interictal periods across patients as control. Our results characterize network features that quantify the underlying functional dynamics associated with the observed heterogeneity of seizure propagation across these two types of focal seizures. Decoding these network features demonstrate that bilateral propagation of seizure activity is an outcome of the imbalance of global integration and segregation in the brain prior to seizure onset. We show that there exist intrinsic network signatures preceding seizure onset that are associated with the extent to which an impending seizure will propagate throughout the brain (i.e. staying within one hemisphere versus spreading transcallosally). Additionally, these features characterize an increase in segregation and a decrease in excitability within the brain network (i.e. high modularity and low spectral radius). Importantly, seizure-type-specific differences in these features emerge several minutes prior to seizure onset, suggesting the potential utility of such measures in intervention strategies. Finally, our results reveal network characteristics the onset that are unique to the propagation mechanisms of two most common focal seizure subtypes, indicative of distinct reconfiguration processes that may assist termination of each seizure type. Together, our findings provide insights into the relationship between the temporal evolution of seizure activity and the underlying functional connectivity dynamics. These results offer exciting avenues where graph-theoretical measures could potentially guide personalized clinical interventions for epilepsy and other neurological disorders in which extensive heterogeneity is observed across subtypes as well as across and within individual patients.

Linker, S.B., Narvaiza, I., Hsu, J.Y., Wang, M., Qiu, F., Mendes, A.P.D., Oefner, R., Kottilil, K., Sharma, A., Randolph-Moore, L., Mejia, E., Santos, R., Marchetto, M.C., Gage, F.H. Regulación específica de humanos en la maduración neural identificada mediante transcriptómica comparada entre primates. (2022) Biología Actual. DOI: 10.1016/j.cub.2022.09.028

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Martins, L.F., Brambilla, I., Motta, A., de Pretis, S., Bhat, G.P., Badaloni, A., Malpighi, C., Amin, N.D., Imai, F., Almeida, R.D., Yoshida, Y., Pfaff, S.L., Bonanomi, D. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity. (2022) Neurona. DOI: 10.1016/j.neuron.2022.09.021

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van den Hurk, M., Lau, S., Marchetto, M.C., Mertens, J., Stern, S., Corti, O., Brice, A., Winner, B., Winkler, J., Gage, F.H., Bardy, C. Vías transcriptómicas tratables reveladas en neuronas de mesencéfalo derivadas de pacientes con Parkinson. (2022) NPJ Parkinson's Dis. 8(1):134. DOI: 10.1038/s41531-022-00400-0

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Las complejas predisposiciones genéticas aceleran la degeneración crónica de las neuronas de la sustancia negra del mesencéfalo en la enfermedad de Parkinson (EP). Descifrar la composición molecular humana de la fisiopatología de la EP puede orientar el descubrimiento de tratamientos para frenar la progresión de la enfermedad. Sin embargo, los hallazgos de los estudios de cerebros humanos post mortem solo reflejan las etapas finales de la EP, y hay una falta de datos sobre los eventos moleculares que preceden a la pérdida neuronal en los pacientes. Abordamos esta brecha identificando la desregulación génica de neuronas vivas del mesencéfalo reprogramadas in vitro a partir de células cutáneas de 42 individuos, incluyendo pacientes con EP esporádica y familiar y controles sanos emparejados. Para minimizar el sesgo resultante de la reprogramación neuronal y los métodos de secuenciación de ARN, desarrollamos un proceso de análisis que integra transcriptomas de la EP procedentes de diferentes conjuntos de datos de secuenciación de ARN (a granel sin clasificar y clasificado frente a de célula única y Patch-seq) y estrategias de reprogramación (pluripotencia inducida frente a conversión directa). El transcriptoma de esta cohorte de EP está enriquecido con genes humanos asociados a fenotipos clínicos conocidos de la EP, la regulación de la locomoción, la bradicinesia y la rigidez. La expresión génica desregulada se manifiesta con mayor intensidad en las vías subyacentes a la transmisión sináptica, el metabolismo, el tráfico intracelular, la morfogénesis neural y el estrés celular/las respuestas inmunitarias. Confirmamos un deterioro sináptico mediante patch-clamping e identificamos los pesticidas y los estresantes del retículo endoplásmico como las interacciones gen-químicas más significativas en la EP. Posteriormente, asociamos el perfil transcriptómico de la EP con fármacos candidatos en una gran base de datos y un registro de ensayos clínicos actuales. Este estudio destaca las vías transcriptómicas humanas que pueden ser objeto de tratamiento terapéutico antes de la pérdida neuronal irreversible. Además, demuestra la relevancia preclínica de los ensayos transcriptómicos imparciales a gran escala de neuronas de pacientes reprogramadas.

Li, P., Garg, A.K., Zhang, L.A., Rashid, M.S., Callaway, E.M. Cone opponent functional domains in primary visual cortex combine signals for color appearance mechanisms. (2022) Nature Communications. 13(1):6344. DOI: 10.1038/s41467-022-34020-2

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Wang, H., Dey, O., Lagos, W.N., Callaway, E.M. Diversity in spatial frequency, temporal frequency, and speed tuning across mouse visual cortical areas and layers. (2022) Journal of Comparative Neurology. DOI: 10.1002/cne.25404

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Pașca, S.P., Arlotta, P., Bateup, H.S., Camp, J.G., Cappello, S., Gage, F.H., Knoblich, J.A., Kriegstein, A.R., Lancaster, M.A., Ming, G.L., Muotri, A.R., Park, I.H., Reiner, O., Song, H., Studer, L., Temple, S., Testa, G., Treutlein, B., Vaccarino, F.M. Un consenso de nomenclatura para organoides y ensambloides del sistema nervioso. (2022) Naturaleza. 609(7929):907-910. DOI: 10.1038/s41586-022-05219-6

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Stern, S., Lau, S., Manole, A., Rosh, I., Percia, M.M., Ben Ezer, R., Shokhirev, M.N., Qiu, F., Schafer, S., Mansour, A.A., Mangan, K.P., Stern, T., Ofer, P., Stern, Y., Diniz Mendes, A.P., Djamus, J., Moore, L.R., Nayak, R., Laufer, S.H., Aicher, A., Rhee, A., Wong, T.L., Nguyen, T., Linker, S.B., Winner, B., Freitas, B.C., Jones, E., Sagi, I., Bardy, C., Brice, A., Winkler, J., Marchetto, M.C., Gage, F.H. Actividad sináptica reducida y vías de matriz extracelular desreguladas en neuronas del mesencéfalo de pacientes con enfermedad de Parkinson. (2022) NPJ Parkinson's Dis. 8(1):103. DOI: 10.1038/s41531-022-00366-z

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Traxler, L., Herdy, J.R., Stefanoni, D., Eichhorner, S., Pelucchi, S., Szücs, A., Santagostino, A., Kim, Y., Agarwal, R.K., Schlachetzki, J.C.M., Glass, C.K., Lagerwall, J., Galasko, D., Gage, F.H., D'Alessandro, A., Mertens, J. La transformación metabólica similar a la de Warburg subyace a la degeneración neuronal en la enfermedad de Alzheimer esporádica. (2022) Metabolismo Celular. DOI: 10.1016/j.cmet.2022.07.014

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Ghaninia, M., Zhou, Y., Knauer, A.C., Schiestl, F.P., Sharpee, T.O., Smith, B.H. Hyperbolic odorant mixtures as a basis for more efficient signaling between flowering plants and bees. (2022) PLOS One. 17(7):e0270358. DOI: 10.1371/journal.pone.0270358

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van Niekerk, E.A., Kawaguchi, R., Marques de Freria, C., Groeniger, K., Marchetto, M.C., Dupraz, S., Bradke, F., Geschwind, D.H., Gage, F.H., Tuszynski, M.H. Los métodos para cultivar neuronas del SNC adulto revelan un efecto de acondicionamiento del SNC. (2022) Cell Rep Methods. 2(7):100255. DOI: 10.1016/j.crmeth.2022.100255

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Bae, T., Fasching, L., Wang, Y., Shin, J.H., Suvakov, M., Jang, Y., Norton, S., Dias, C., Mariani, J., Jourdon, A., Wu, F., Panda, A., Pattni, R., Chahine, Y., Yeh, R., Roberts, R.C., Huttner, A., Kleinman, J.E., Hyde, T.M., Straub, R.E., Walsh, C.A., , Urban, A.E., Leckman, J.F., Weinberger, D.R., Vaccarino, F.M., Abyzov, A., Walsh, C.A., Park, P.J., Sestan, N., Weinberger, D., Moran, J.V., Gage, F.H., Vaccarino, F.M., Gleeson, J., Mathern, G., Courchesne, E., Roy, S., Chess, A.J., Akbarian, S., Bizzotto, S., Coulter, M., Dias, C., D'Gama, A., Ganz, J., Hill, R., Huang, A.Y., Khoshkhoo, S., Kim, S., Lee, A., Lodato, M., Maury, E.A., Miller, M., Borges-Monroy, R., Rodin, R., Zhou, Z., Bohrson, C., Chu, C., Cortes-Ciriano, I., Dou, Y., Galor, A., Gulhan, D., Kwon, M., Luquette, J., Sherman, M., Viswanadham, V., Jones, A., Rosenbluh, C., Cho, S., Langmead, B., Thorpe, J., Erwin, J., Jaffe, A., McConnell, M., Narurkar, R., Paquola, A., Shin, J., Straub, R., Abyzov, A., Bae, T., Jang, Y., Wang, Y., Molitor, C., Peters, M., Linker, S., Reed, P., Wang, M., Urban, A., Zhou, B., Zhu, X., Pattni, R., Serres Amero, A., Juan, D., Lobon, I., Marques-Bonet, T., Solis Moruno, M., Garcia Perez, R., Povolotskaya, I., Soriano, E., Antaki, D., Averbuj, D., Ball, L., Breuss, M., Yang, X., Chung, C., Emery, S.B., Flasch, D.A., Kidd, J.M., Kopera, H.C., Kwan, K.Y., Mills, R.E., Moldovan, J.B., Sun, C., Zhao, X., Zhou, W., Frisbie, T.J., Cherskov, A., Fasching, L., Jourdon, A., Pochareddy, S., Scuderi, S. El análisis de mutaciones somáticas en 131 cerebros humanos revela hipermutabilidad asociada al envejecimiento. (2022) Ciencia. 377(6605):511-517. DOI: 10.1126/science.abm6222

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Lecoin, L., Dempsey, B., Garancher, A., Bourane, S., Ruffault, P.L., Morin-Surun, M.P., Rocques, N., Goulding, M., Eychène, A., Pouponnot, C., Fortin, G., Champagnat, J. Mafa-dependent GABAergic activity promotes mouse neonatal apneas. (2022) Nature Communications. 13(1):3284. DOI: 10.1038/s41467-022-30825-3

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Ward, S., Riley, C., Carey, E.M., Nguyen, J., Esener, S., Nimmerjahn, A., Sirbuly, D.J. Electro-optical mechanically flexible coaxial microprobes for minimally invasive interfacing with intrinsic neural circuits. (2022) Nature Communications. 13(1):3286. DOI: 10.1038/s41467-022-30275-x

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Zhang, Y., Roy, D.S., Zhu, Y., Chen, Y., Aida, T., Hou, Y., Shen, C., Lea, N.E., Schroeder, M.E., Skaggs, K.M., Sullivan, H.A., Fischer, K.B., Callaway, E.M., Wickersham, I.R., Dai, J., Li, X.M., Lu, Z., Feng, G. Targeting thalamic circuits rescues motor and mood deficits in PD mice. (2022) Naturaleza. DOI: 10.1038/s41586-022-04806-x

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Chen, Y., Rosen, B.Q., Sejnowski, T.J. Dynamical differential covariance recovers directional network structure in multiscale neural systems. (2022) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 119(24):e2117234119. DOI: 10.1073/pnas.2117234119

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Grün, S., Li, J., McNaughton, B., Petersen, C., McCormick, D., Robson, D., Buzsáki, G., Harris, K., Sejnowski, T., Mrsic-Flogel, T., Lindén, H., Roland, P.E. Emerging principles of spacetime in brains: Meeting report on spatial neurodynamics. (2022) Neurona. 110(12):1894-1898. DOI: 10.1016/j.neuron.2022.05.018

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Chen, Y., Bukhari, Q., Lin, T.W., Sejnowski, T.J. Functional connectivity of fMRI using differential covariance predicts structural connectivity and behavioral reaction times. (2022) Network neuroscience. 6(2):614-633. DOI: 10.1162/netn_a_00239

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Zhou, Y., Sharpee, T.O. Using Global t-SNE to Preserve Intercluster Data Structure. (2022) Computación neuronal.:1-15 DOI: 10.1162/neco_a_01504

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Tadokoro, T., Bravo-Hernandez, M., Agashkov, K., Kobayashi, Y., Platoshyn, O., Navarro, M., Marsala, S., Miyanohara, A., Yoshizumi, T., Shigyo, M., Krotov, V., Juhas, S., Juhasova, J., Nguyen, D., Skalnikova, H.K., Motlik, J., Studenovska, H., Proks, V., Reddy, R., Driscoll, S.P., Glenn, T.D., Kemthong, T., Malaivijitnond, S., Tomori, Z., Vanicky, I., Kakinohana, M., Pfaff, S.L., Ciacci, J., Belan, P., Marsala, M. Precision spinal gene delivery-induced functional switch in nociceptive neurons reverses neuropathic pain. (2022) Molecular Therapy. DOI: 10.1016/j.ymthe.2022.04.023

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Garcia, J.W., Bartol, T.M., Sejnowski, T.J. Multiscale modeling of presynaptic dynamics from molecular to mesoscale. (2022) PLOS Biología Computacional. 18(5):e1010068. DOI: 10.1371/journal.pcbi.1010068

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Chemical synapses exhibit a diverse array of internal mechanisms that affect the dynamics of transmission efficacy. Many of these processes, such as release of neurotransmitter and vesicle recycling, depend strongly on activity-dependent influx and accumulation of Ca2+. To model how each of these processes may affect the processing of information in neural circuits, and how their dysfunction may lead to disease states, requires a computationally efficient modelling framework, capable of generating accurate phenomenology without incurring a heavy computational cost per synapse. Constructing a phenomenologically realistic model requires the precise characterization of the timing and probability of neurotransmitter release. Difficulties arise in that functional forms of instantaneous release rate can be difficult to extract from noisy data without running many thousands of trials, and in biophysical synapses, facilitation of per-vesicle release probability is confounded by depletion. To overcome this, we obtained traces of free Ca2+ concentration in response to various action potential stimulus trains from a molecular MCell model of a hippocampal Schaffer collateral axon. Ca2+ sensors were placed at varying distance from a voltage-dependent calcium channel (VDCC) cluster, and Ca2+ was buffered by calbindin. Then, using the calcium traces to drive deterministic state vector models of synaptotagmin 1 and 7 (Syt-1/7), which respectively mediate synchronous and asynchronous release in excitatory hippocampal synapses, we obtained high-resolution profiles of instantaneous release rate, to which we applied functional fits. Synchronous vesicle release occurred predominantly within half a micron of the source of spike-evoked Ca2+ influx, while asynchronous release occurred more consistently at all distances. Both fast and slow mechanisms exhibited multi-exponential release rate curves, whose magnitudes decayed exponentially with distance from the Ca2+ source. Profile parameters facilitate on different time scales according to a single, general facilitation function. These functional descriptions lay the groundwork for efficient mesoscale modelling of vesicular release dynamics.

Li, J., Pinto-Duarte, A., Zander, M., Cuoco, M.S., Lai, C.Y., Osteen, J., Fang, L., Luo, C., Lucero, J.D., Gomez-Castanon, R., Nery, J.R., Silva-Garcia, I., Pang, Y., Sejnowski, T.J., Powell, S.B., Ecker, J.R., Mukamel, E.A., Behrens, M.M. Dnmt3a knockout in excitatory neurons impairs postnatal synapse maturation and increases the repressive histone modification H3K27me3. (2022) Elife. 11. DOI: 10.7554/eLife.66909

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Patiño, M., Lagos, W.N., Patne, N.S., Tasic, B., Zeng, H., Callaway, E.M. Single-cell transcriptomic classification of rabies-infected cortical neurons. (2022) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 119(22):e2203677119. DOI: 10.1073/pnas.2203677119

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Oliinyk, O.S., Baloban, M., Clark, C.L., Carey, E., Pletnev, S., Nimmerjahn, A., Verkhusha, V.V. Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies. (2022) Nature Methods. DOI: 10.1038/s41592-022-01467-6

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Nimmerjahn, A., Hirrlinger, J. Astrocyte regulation of neural circuit function and animal behavior. (2022) Glia. DOI: 10.1002/glia.24223

Shi, Y., Evans, R.M., Gage, F.H. El ácido oleico regula la neurogénesis hipocampal como un ligando de TLX. (2022) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 119(15):e2203038119. DOI: 10.1073/pnas.2203038119

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Amin, N.D., Senturk, G., Hayashi, M., Driscoll, S.P., Pfaff, S.L. Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations. (2022) STAR Protoc. 3(1):101130. DOI: 10.1016/j.xpro.2022.101130

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Cook, J.R., Li, H., Nguyen, B., Huang, H.H., Mahdavian, P., Kirchgessner, M.A., Strassmann, P., Engelhardt, M., Callaway, E.M., Jin, X. Secondary auditory cortex mediates a sensorimotor mechanism for action timing. (2022) Nature Neuroscience. 25(3):330-344. DOI: 10.1038/s41593-022-01025-5

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Hirrlinger, J., Nimmerjahn, A. A perspective on astrocyte regulation of neural circuit function and animal behavior. (2022) Glia. DOI: 10.1002/glia.24168

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Zhang, Z., Cisneros, E., Lee, H.Y., Vu, J.P., Chen, Q., Benadof, C.N., Whitehill, J., Rouzbehani, R., Sy, D.T., Huang, J.S., Sejnowski, T.J., Jankovic, J., Factor, S., Goetz, C.G., Barbano, R.L., Perlmutter, J.S., Jinnah, H.A., Berman, B.D., Richardson, S.P., Stebbins, G.T., Comella, C.L., Peterson, D.A. Hold that pose: capturing cervical dystonia's head deviation severity from video. (2022) Ann Clin Transl Neurol. DOI: 10.1002/acn3.51549

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Budzinski, R.C., Nguyen, T.T., Đoàn, J., Mináč, J., Sejnowski, T.J., Muller, L.E. Geometry unites synchrony, chimeras, and waves in nonlinear oscillator networks. (2022) Chaos. 32(3):031104. DOI: 10.1063/5.0078791

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Nowotny, T., van Albada, S.J., Fellous, J.M., Haas, J.S., Jolivet, R.B., Metzner, C., Sharpee, T. Editorial: Advances in Computational Neuroscience. (2022) Frontiers in Computational Neuroscience. 15:824899. DOI: 10.3389/fncom.2021.824899

Abdelfattah, A.S., Ahuja, S., Akkin, T., Allu, S.R., Brake, J., Boas, D.A., Buckley, E.M., Campbell, R.E., Chen, A.I., Cheng, X., Čižmár, T., Costantini, I., De Vittorio, M., Devor, A., Doran, P.R., El Khatib, M., Emiliani, V., Fomin-Thunemann, N., Fainman, Y., Fernandez-Alfonso, T., Ferri, C.G.L., Gilad, A., Han, X., Harris, A., Hillman, E.M.C., Hochgeschwender, U., Holt, M.G., Ji, N., Kılıç, K., Lake, E.M.R., Li, L., Li, T., Mächler, P., Miller, E.W., Mesquita, R.C., Nadella, K.M.N.S., Nägerl, U.V., Nasu, Y., Nimmerjahn, A., Ondráčková, P., Pavone, F.S., Perez Campos, C., Peterka, D.S., Pisano, F., Pisanello, F., Puppo, F., Sabatini, B.L., Sadegh, S., Sakadzic, S., Shoham, S., Shroff, S.N., Silver, R.A., Sims, R.R., Smith, S.L., Srinivasan, V.J., Thunemann, M., Tian, L., Tian, L., Troxler, T., Valera, A., Vaziri, A., Vinogradov, S.A., Vitale, F., Wang, L.V., Uhlířová, H., Xu, C., Yang, C., Yang, M.H., Yellen, G., Yizhar, O., Zhao, Y. Neurophotonic tools for microscopic measurements and manipulation: status report. (2022) Neurophotonics. 9(Suppl 1):013001. DOI: 10.1117/1.NPh.9.S1.013001

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Gage, F.H. Neurogénesis adulta en enfermedades neurológicas. [Pubmed] (2021) Ciencia. 374(6571):1049-1050. DOI: 10.1126/science.abm7468

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Heard, K.J., Shokhirev, M.N., Becronis, C., Fredlender, C., Zahid, N., Le, A.T., Ji, Y., Skime, M., Nelson, T., Hall-Flavin, D., Weinshilboum, R., Gage, F.H., Vadodaria, K.C. El cortisol crónico impacta de manera diferencial las astrocitos derivadas de células madre de pacientes con trastorno depresivo mayor. (2021) Psiquiatría Traducida. 11(1):608. DOI: 10.1038/s41398-021-01733-9

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Wagner, M., Bartol, T.M., Sejnowski, T.J., Cauwenberghs, G. Markov Chain Abstractions of Electrochemical Reaction-Diffusion in Synaptic Transmission for Neuromorphic Computing. (2021) Fronteras en Neurociencia. 15:698635. DOI: 10.3389/fnins.2021.698635

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Mendelsohn, R., Garcia, G.C., Bartol, T.M., Lee, C.T., Khandelwal, P., Liu, E., Spencer, D.J., Husar, A., Bushong, E.A., Phan, S., Perkins, G., Ellisman, M.H., Skupin, A., Sejnowski, T.J., Rangamani, P. Morphological principles of neuronal mitochondria. (2021) Journal of Comparative Neurology. DOI: 10.1002/cne.25254

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Liu, H., Zhou, J., Tian, W., Luo, C., Bartlett, A., Aldridge, A., Lucero, J., Osteen, J.K., Nery, J.R., Chen, H., Rivkin, A., Castanon, R.G., Clock, B., Li, Y.E., Hou, X., Poirion, O.B., Preissl, S., Pinto-Duarte, A., O'Connor, C., Boggeman, L., Fitzpatrick, C., Nunn, M., Mukamel, E.A., Zhang, Z., Callaway, E.M., Ren, B., Dixon, J.R., Behrens, M.M., Ecker, J.R. DNA methylation atlas of the mouse brain at single-cell resolution. (2021) Naturaleza. 598(7879):120-128. DOI: 10.1038/s41586-020-03182-8

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Zhang, Z., Zhou, J., Tan, P., Pang, Y., Rivkin, A.C., Kirchgessner, M.A., Williams, E., Lee, C.T., Liu, H., Franklin, A.D., Miyazaki, P.A., Bartlett, A., Aldridge, A.I., Vu, M., Boggeman, L., Fitzpatrick, C., Nery, J.R., Castanon, R.G., Rashid, M., Jacobs, M.W., Ito-Cole, T., O'Connor, C., Pinto-Duartec, A., Dominguez, B., Smith, J.B., Niu, S.Y., Lee, K.F., Jin, X., Mukamel, E.A., Behrens, M.M., Ecker, J.R., Callaway, E.M. Epigenomic diversity of cortical projection neurons in the mouse brain. (2021) Naturaleza. 598(7879):167-173. DOI: 10.1038/s41586-021-03223-w

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Neuronal cell types are classically defined by their molecular properties, anatomy and functions. Although recent advances in single-cell genomics have led to high-resolution molecular characterization of cell type diversity in the brain, neuronal cell types are often studied out of the context of their anatomical properties. To improve our understanding of the relationship between molecular and anatomical features that define cortical neurons, here we combined retrograde labelling with single-nucleus DNA methylation sequencing to link neural epigenomic properties to projections. We examined 11,827 single neocortical neurons from 63 cortico-cortical and cortico-subcortical long-distance projections. Our results showed unique epigenetic signatures of projection neurons that correspond to their laminar and regional location and projection patterns. On the basis of their epigenomes, intra-telencephalic cells that project to different cortical targets could be further distinguished, and some layer 5 neurons that project to extra-telencephalic targets (L5 ET) formed separate clusters that aligned with their axonal projections. Such separation varied between cortical areas, which suggests that there are area-specific differences in L5 ET subtypes, which were further validated by anatomical studies. Notably, a population of cortico-cortical projection neurons clustered with L5 ET rather than intra-telencephalic neurons, which suggests that a population of L5 ET cortical neurons projects to both targets. We verified the existence of these neurons by dual retrograde labelling and anterograde tracing of cortico-cortical projection neurons, which revealed axon terminals in extra-telencephalic targets including the thalamus, superior colliculus and pons. These findings highlight the power of single-cell epigenomic approaches to connect the molecular properties of neurons with their anatomical and projection properties.

Davis, Z.W., Benigno, G.B., Fletterman, C., Desbordes, T., Steward, C., Sejnowski, T.J., Reynolds, J.H., Muller, L. Spontaneous traveling waves naturally emerge from horizontal fiber time delays and travel through locally asynchronous-irregular states. (2021) Nature Communications. 12(1):6057. DOI: 10.1038/s41467-021-26175-1

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Li, Y., Kim, R., Sejnowski, T.J. Learning the Synaptic and Intrinsic Membrane Dynamics Underlying Working Memory in Spiking Neural Network Models. (2021) Computación neuronal.:1-24 DOI: 10.1162/neco_a_01409

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Lainscsek, C., Cash, S.S., Sejnowski, T.J., Kurths, J. Dynamical ergodicity DDA reveals causal structure in time series. (2021) Chaos. 31(10):103108. DOI: 10.1063/5.0063724

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Kirchgessner, M.A., Franklin, A.D., Callaway, E.M. Distinct "driving" versus "modulatory" influences of different visual corticothalamic pathways. (2021) Biología Actual. DOI: 10.1016/j.cub.2021.09.025

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Gorbunova, V., Seluanov, A., Mita, P., McKerrow, W., Fenyö, D., Boeke, J.D., Linker, S.B., Gage, F.H., Kreiling, J.A., Petrashen, A.P., Woodham, T.A., Taylor, J.R., Helfand, S.L., Sedivy, J.M. El papel de los elementos retrotransponibles en el envejecimiento y las enfermedades asociadas a la edad. https://rdcu.be/ct1i3 Texto completo proporcionado por la iniciativa de intercambio de contenido Springer Nature SharedIt [Pubmed] (2021) Naturaleza. 596(7870):43-53. DOI: 10.1038/s41586-021-03542-Y

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Shadel, G.S., Adams, P.D., Berggren, W.T., Diedrich, J.K., Diffenderfer, K.E., Gage, F.H., Hah, N., Hansen, M., Hetzer, M.W., Molina, A.J.A., Manor, U., Marek, K., O'Keefe, D.D., Pinto, A.F.M., Sacco, A., Sharpee, T.O., Shokriev, M.N., Zambetti, S. El Centro Nathan Shock de San Diego: abordando la heterogeneidad del envejecimiento. (2021) Gerociencia. DOI: 10.1007/s11357-021-00426-x

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Comprender los mecanismos básicos del envejecimiento ofrece grandes perspectivas para desarrollar intervenciones que prevengan o retrasen simultáneamente muchos deterioros y enfermedades relacionados con la edad, con el fin de prolongar la esperanza de vida saludable. Sin embargo, un importante factor de confusión en la investigación sobre el envejecimiento es la heterogeneidad del propio proceso de envejecimiento. A nivel del organismo, está claro que la edad cronológica no siempre predice la edad biológica ni la susceptibilidad a la fragilidad o la patología. Si bien la genética y el entorno son factores importantes que determinan las tasas variables de envejecimiento, surge una complejidad adicional debido a que los diferentes órganos, tejidos y tipos de células son intrínsecamente heterogéneos y presentan diferentes trayectorias de envejecimiento, ya sea de manera normal o en respuesta a las tensiones del proceso de envejecimiento (por ejemplo, la acumulación de daños). Abordar la heterogeneidad del envejecimiento requiere herramientas nuevas y especializadas (por ejemplo, análisis de células individuales, enfoques basados en la espectrometría de masas e imágenes avanzadas) para identificar nuevas firmas del envejecimiento en todas las escalas. A continuación, se necesitan enfoques computacionales de vanguardia para integrar estos conjuntos de datos dispares y dilucidar las interacciones en red entre los rasgos característicos conocidos del envejecimiento. También existe la necesidad de modelos mejorados de envejecimiento basados en células humanas para garantizar que los hallazgos de la investigación básica sean relevantes para el envejecimiento humano y las intervenciones para prolongar la vida saludable. El San Diego Nathan Shock Center (SD-NSC) brinda acceso a recursos científicos de vanguardia para facilitar el estudio de la heterogeneidad del envejecimiento en general y promover el uso de nuevos modelos de envejecimiento con células humanas. El centro también cuenta con un sólido Núcleo de Desarrollo de Investigación que financia proyectos piloto sobre la heterogeneidad del envejecimiento y organiza actividades de capacitación innovadoras, incluyendo talleres y un programa de tutoría personalizada, para ayudar a los investigadores nuevos en el campo del envejecimiento a tener éxito. Finalmente, el SD-NSC participa en actividades de divulgación para educar a la comunidad en general sobre la importancia de la investigación sobre el envejecimiento y promover la necesidad de la investigación en biología básica del envejecimiento en particular.

Amin, N.D., Senturk, G., Costaguta, G., Driscoll, S., O'Leary, B., Bonanomi, D., Pfaff, S.L. A hidden threshold in motor neuron gene networks revealed by modulation of miR-218 dose. (2021) Neurona. DOI: 10.1016/j.neuron.2021.07.028

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Hayes, T.L., Krishnan, G.P., Bazhenov, M., Siegelmann, H.T., Sejnowski, T.J., Kanan, C. Replay in Deep Learning: Current Approaches and Missing Biological Elements. (2021) Computación neuronal.:1-44 DOI: 10.1162/neco_a_01433

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Johnston, S., Parylak, S., Kim, S., Mac, N., Lim, C., Gallina, I., Bloyd, C., Newberry, A., Saavedra, C.D., Novak, O., Goncalves, J.T., Gage, F.H., Shtrahman, M. AAV abla la neurogénesis en el hipocampo murino adulto. (2021) Elife. 10. DOI: 10.7554/eLife.59291

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Nakahira, Y., Liu, Q., Sejnowski, T.J., Doyle, J.C. Diversity-enabled sweet spots in layered architectures and speed-accuracy trade-offs in sensorimotor control. (2021) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 118(22). DOI: 10.1073/pnas.1916367118

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Tan, K., Kim, M.E., Song, H.W., Skarbrevik, D., Babajanian, E., Bedrosian, T.A., Gage, F.H., Wilkinson, M.F. El clúster de genes Rhox suprime la transposición de la línea germinal LINE1. (2021) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 118(23). DOI: 10.1073/pnas.2024785118

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Faulkner, R.L., Wall, N.R., Callaway, E.M., Cline, H.T. Application of Recombinant Rabies Virus to Xenopus Tadpole Brain. (2021) eNeuro. DOI: 10.1523/ENEURO.0477-20.2021

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The Xenopus laevis experimental system has provided significant insight into the development and plasticity of neural circuits. Xenopus neuroscience research would be enhanced by additional tools to study neural circuit structure and function. Rabies viruses are powerful tools to label and manipulate neural circuits and have been widely used to study mesoscale connectomics. Whether rabies virus can be used to transduce neurons and express transgenes in Xenopus has not been systematically investigated. Glycoprotein-deleted rabies virus transduces neurons at the axon terminal and retrogradely labels their cell bodies. We show that glycoprotein-deleted rabies virus infects local and projection neurons in the Xenopus tadpole when directly injected into brain tissue. Pseudotyping glycoprotein-deleted rabies with EnvA restricts infection to cells with exogenous expression of the EnvA receptor, TVA. EnvA pseudotyped virus specifically infects tadpole neurons with promoter-driven expression of TVA, demonstrating its utility to label targeted neuronal populations. Neuronal cell types are defined by a combination of features including anatomical location, expression of genetic markers, axon projection sites, morphology, and physiological properties. We show that driving TVA expression in one hemisphere and injecting EnvA pseudotyped virus into the contralateral hemisphere, retrogradely labels neurons defined by cell body location and axon projection site. Using this approach, rabies can be used to identify cell types in Xenopus brain and simultaneously to express transgenes which enable monitoring or manipulation of neuronal activity. This makes rabies a valuable tool to study the structure and function of neural circuits in Xenopus.Studies in Xenopus have contributed a great deal to our understanding of brain circuit development and plasticity, regeneration, and hormonal regulation of behavior and metamorphosis. Here, we show that recombinant rabies virus transduces neurons in the Xenopus tadpole, enlarging the toolbox that can be applied to studying Xenopus brain. Rabies can be used for retrograde labeling and expression of a broad range of transgenes including fluorescent proteins for anatomical tracing and studying neuronal morphology, voltage or calcium indicators to visualize neuronal activity, and photo- or chemosensitive channels to control neuronal activity. The versatility of these tools enables diverse experiments to analyze and manipulate Xenopus brain structure and function, including mesoscale connectivity.

Schafer, S.T., Gage, F.H. Comer o no comer, esa es la cuestión: las células madre neurales escapan a la fagocitosis en el autismo con macrocefalia. (2021) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 118(19). DOI: 10.1073/pnas.2104888118

Compans, B., Camus, C., Kallergi, E., Sposini, S., Martineau, M., Butler, C., Kechkar, A., Klaassen, R.V., Retailleau, N., Sejnowski, T.J., Smit, A.B., Sibarita, J.B., Bartol, T.M., Perrais, D., Nikoletopoulou, V., Choquet, D., Hosy, E. NMDAR-dependent long-term depression is associated with increased short term plasticity through autophagy mediated loss of PSD-95. (2021) Nature Communications. 12(1):2849. DOI: 10.1038/s41467-021-23133-9

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Dias, G.P., Murphy, T., Stangl, D., Ahmet, S., Morisse, B., Nix, A., Aimone, L.J., Aimone, J.B., Kuro-O, M., Gage, F.H., Thuret, S. El ayuno intermitente mejora la consolidación de la memoria a largo plazo, la neurogénesis del hipocampo en adultos y la expresión del gen de longevidad Klotho. (2021) Psiquiatría Molecular. DOI: 10.1038/s41380-021-01102-4

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Hsu, W.M., Kastner, D.B., Baccus, S.A., Sharpee, T.O. How inhibitory neurons increase information transmission under threshold modulation. (2021) Cell Reports. 35(8):109158. DOI: 10.1016/j.celrep.2021.109158

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Reid, D.A., Reed, P.J., Schlachetzki, J.C.M., Nitulescu, I.I., Chou, G., Tsui, Chandran, S., Jones, J.R., Lu, A.T., McClain, C.A., Ooi, J.H., Wang, T.W., Lana, Linker, S.B., Ricciardulli, A.S., Lau, S., Schafer, S., Horvath, S., Dixon, J.R., Hah, N., Glass, C.K., Gage, F.H. La incorporación de un análogo de nucleósido mapea sitios de reparación del genoma en neuronas humanas postmitóticas. (2021) Ciencia. 372(6537):91-94. DOI: 10.1126/science.abb9032

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Huang, Y., Happonen, K.E., Burrola, P.G., O'Connor, C., Hah, N., Huang, L., Nimmerjahn, A., Lemke, G. Microglia use TAM receptors to detect and engulf amyloid β plaques. (2021) Naturaleza Inmunológica. DOI: 10.1038/s41590-021-00913-5

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Osseward, P.J., Amin, N.D., Moore, J.D., Temple, B.A., Barriga, B.K., Bachmann, L.C., Beltran, F., Gullo, M., Clark, R.C., Driscoll, S.P., Pfaff, S.L., Hayashi, M. Conserved genetic signatures parcellate cardinal spinal neuron classes into local and projection subsets. (2021) Ciencia. 372(6540):385-393. DOI: 10.1126/science.abe0690

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Mertens, J., Herdy, J.R., Traxler, L., Schafer, S.T., Schlachetzki, J.C.M., Böhnke, L., Reid, D.A., Lee, H., Zangwill, D., Fernandes, D.P., Agarwal, R.K., Lucciola, R., Zhou-Yang, L., Karbacher, L., Edenhofer, F., Stern, S., Horvath, S., Paquola, A.C.M., Glass, C.K., Yuan, S.H., Ku, M., Szücs, A., Goldstein, L.S.B., Galasko, D., Gage, F.H. Inestabilidad dependiente de la edad del destino neuronal maduro en neuronas inducidas de pacientes con Alzheimer. (2021) Células Madre. DOI: 10.1016/j.stem.2021.04.004

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Schafer, S.T., Gage, F.H. El cuándo y el dónde: Convergencia molecular y celular en el autismo. (2021) Psiquiatría Biológica. 89(5):419-420. DOI: 10.1016/j.biopsych.2020.12.016

Mishra, H.K., Ying, N.M., Luis, A., Wei, H., Nguyen, M., Nakhla, T., Vandenburgh, S., Alda, M., Berrettini, W.H., Brennand, K.J., Calabrese, J.R., Coryell, W.H., Frye, M.A., Gage, F.H., Gershon, E.S., McInnis, M.G., Nievergelt, C.M., Nurnberger, J.I., Shilling, P.D., Oedegaard, K.J., Zandi, P.P., , Kelsoe, J.R., Welsh, D.K., McCarthy, M.J. Los ritmos circadianos en neuronas derivadas de pacientes con trastorno bipolar predicen la respuesta al litio: estudios preliminares. (2021) Psiquiatría Molecular. DOI: 10.1038/s41380-021-01048-7

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El trastorno bipolar (TB) es una enfermedad neuropsiquiátrica caracterizada por episodios recurrentes de manía/hipomanía, depresión y alteraciones del ritmo circadiano. El litio es un fármaco eficaz para el TB, pero entre el 30 % y el 40 % de los pacientes no responden adecuadamente al tratamiento. Estudios previos han demostrado que el litio afecta la expresión de los "genes del reloj" y que los pacientes que responden al litio (Li-R) pueden distinguirse de los que no responden (Li-NR) por diferencias en los ritmos circadianos. Sin embargo, los ritmos circadianos no se han evaluado en las neuronas de pacientes con TB de los grupos Li-R y Li-NR. Utilizamos células madre pluripotentes inducidas (iPSC) para cultivar células precursoras neuronales (NPC) y neuronas glutamatérgicas de pacientes con TB caracterizados por su respuesta al litio y controles emparejados. Identificamos ritmos circadianos marcados en la expresión de Per2-luc en las NPC y las neuronas de los controles y los Li-R, pero los ritmos de las NPC en los Li-R tenían un período circadiano más corto. Los ritmos de los Li-NR eran de baja amplitud y estaban profundamente debilitados. En las NPC y las neuronas, la expresión de PER2 fue mayor en ambos grupos de TB en comparación con los controles. En las neuronas, los niveles de proteína PER2 fueron mayores en el TB que en los controles, especialmente en las muestras de Li-NR. En células individuales, los ritmos de las NPC y las neuronas en ambos grupos de TB estaban desincronizados en comparación con los controles. El litio alargó el período en las neuronas Li-R y de control, pero no logró alterar los ritmos en Li-NR. Por el contrario, la sincronización por temperatura aumentó la amplitud en todos los grupos y restauró parcialmente los ritmos en las neuronas Li-NR. Concluimos que las anomalías del ritmo circadiano neuronal están presentes en el TB y son más pronunciadas en Li-NR. Los déficits de ritmo en el TB pueden ser parcialmente reversibles mediante la estimulación de las vías de sincronización.

Zhou, Y., Sharpee, T.O. Hyperbolic geometry of gene expression. (2021) iCiencia. 24(3):102225. DOI: 10.1016/j.isci.2021.102225

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Nakatsuka, N., Heard, K.J., Faillétaz, A., Momotenko, D., Vörös, J., Gage, F.H., Vadodaria, K.C. Detección de serotonina secretada por neuronas serotoninérgicas humanas utilizando nanopipetas modificadas con aptámeros. (2021) Psiquiatría Molecular. DOI: 10.1038/s41380-021-01066-5

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El sistema serotoninérgico del cerebro humano modula varios procesos fisiológicos, y se ha relacionado la alteración de la neurotransmisión serotoninérgica con la neuropatología de varios trastornos psiquiátricos. El estudio de la neurotransmisión serotoninérgica en psiquiatría se ha limitado durante mucho tiempo a modelos animales, pero los avances en la tecnología de reprogramación celular han permitido generar neuronas serotoninérgicas a partir de células madre pluripotentes inducidas (iPSC) de pacientes. Si bien las neuronas serotoninérgicas humanas derivadas de iPSC ofrecen la posibilidad de estudiar la liberación y la recaptación de serotonina (5-HT), en particular por fármacos moduladores de la 5-HT como los inhibidores selectivos de la recaptación de serotonina (ISRS), una limitación importante es la incapacidad de cuantificar de manera confiable la 5-HT secretada por las neuronas in vitro. En este trabajo, abordamos esta brecha técnica mediante una novedosa tecnología de detección que acopla aptámeros de ADN específicos para la 5-HT a nanoporos (nanopipetas de vidrio) con orificios de ~10 nm para detectar la 5-HT en un medio de cultivo neuronal complejo con mayor selectividad, sensibilidad y estabilidad que los métodos existentes. Los aptámeros de 5-HT sufren un reordenamiento conformacional al capturar el objetivo y actúan como guardianes del flujo iónico a través de la abertura de la nanopipeta. Generamos neuronas serotoninérgicas humanas in vitro y detectamos la 5-HT secretada utilizando nanopipetas recubiertas de aptámeros en un rango nanomolar bajo, con la posibilidad de detectar concentraciones significativamente más bajas (picomolares). Además, como prueba de concepto, tratamos neuronas serotoninérgicas humanas in vitro con el ISRS citalopram y detectamos un aumento significativo de la 5-HT extracelular utilizando las nanopipetas modificadas con aptámeros. Demostramos la utilidad de dichos métodos para la detección de 5-HT, lo que abre la posibilidad de una cuantificación rápida de los neurotransmisores secretados por células neuronales vivas derivadas de pacientes.

Bedrosian, T.A., Houtman, J., Eguiguren, J.S., Ghassemzadeh, S., Rund, N., Novaresi, N.M., Hu, L., Parylak, S.L., Denli, A.M., Randolph-Moore, L., Namba, T., Gage, F.H., Toda, T. La disminución de la Lamin B1 subyace a la pérdida relacionada con la edad de la neurogénesis hipocampal adulta. (2021) Revista EMBO. 40(3):e105819. DOI: 10.15252/embj.2020105819

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Fukuda, S., Varshney, A., Fowler, B.J., Wang, S.B., Narendran, S., Ambati, K., Yasuma, T., Magagnoli, J., Leung, H., Hirahara, S., Nagasaka, Y., Yasuma, R., Apicella, I., Pereira, F., Makin, R.D., Magner, E., Liu, X., Sun, J., Wang, M., Baker, K., Marion, K.M., Huang, X., Baghdasaryan, E., Ambati, M., Ambati, V.L., Pandey, A., Pandya, L., Cummings, T., Banerjee, D., Huang, P., Yerramothu, P., Tolstonog, G.V., Held, U., Erwin, J.A., Paquola, A.C.M., Herdy, J.R., Ogura, Y., Terasaki, H., Oshika, T., Darwish, S., Singh, R.K., Mozaffari, S., Bhattarai, D., Kim, K.B., Hardin, J.W., Bennett, C.L., Hinton, D.R., Hanson, T.E., Röver, C., Parang, K., Kerur, N., Liu, J., Werner, B.C., Sutton, S.S., Sadda, S.R., Schumann, G.G., Gelfand, B.D., Gage, F.H., Ambati, J. Síntesis citoplasmática de ADN complementario endógeno mediante transcripción inversa e implicaciones en la degeneración macular asociada a la edad. (2021) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 118(6). DOI: 10.1073/pnas.2022751118

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Singh, N., Bartol, T., Levine, H., Sejnowski, T., Nadkarni, S. Presynaptic endoplasmic reticulum regulates short-term plasticity in hippocampal synapses. (2021) Comunicaciones Biología. 4(1):241. DOI: 10.1038/s42003-021-01761-7

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Vadodaria, K.C., Mendes, A.P.D., Mei, A., Racha, V., Erikson, G., Shokhirev, M.N., Oefner, R., Heard, K.J., McCarthy, M.J., Eyler, L., Kelsoe, J.R., Santos, R., Marchetto, M.C., Gage, F.H. Soporte Neuronal Alterado y Respuesta Inflamatoria en Astrocitos Derivados de Pacientes con Trastorno Bipolar. (2021) Stem Cell Reports. DOI: 10.1016/j.stemcr.2021.02.004

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Santos, R., Linker, S.B., Stern, S., Mendes, A.P.D., Shokhirev, M.N., Erikson, G., Randolph-Moore, L., Racha, V., Kim, Y., Kelsoe, J.R., Bang, A.G., Alda, M., Marchetto, M.C., Gage, F.H. La expresión deficiente de LEF1 se asocia con resistencia al litio e hiperexcitabilidad en neuronas derivadas de pacientes con trastorno bipolar. (2021) Psiquiatría Molecular. DOI: 10.1038/s41380-020-00981-3

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El trastorno bipolar (TB) es una afección psiquiátrica caracterizada por episodios depresivos y maníacos que afecta al 21 % de la población mundial. El tratamiento de primera línea a largo plazo para la estabilización del estado de ánimo es el litio (Li). El modelado del TB mediante células madre pluripotentes inducidas, utilizando neuronas similares al giro dentado del hipocampo derivadas de pacientes sensibles al litio (LR) y no sensibles al litio (NR), mostró previamente una hiperexcitabilidad neuronal. El tratamiento con litio revirtió la hiperexcitabilidad solo en las neuronas LR. En este estudio buscamos dianas específicas de la resistencia al litio en las neuronas NR y descubrimos que la actividad de la vía de señalización Wnt/β-catenina se veía gravemente afectada, con una disminución significativa en la expresión de LEF1. El litio actúa sobre la vía de señalización Wnt/β-catenina inhibiendo la GSK-3β y liberando β-catenina, que forma un complejo nuclear con TCF/LEF1, activando así el programa de transcripción Wnt/β-catenina. Por lo tanto, proponemos que la regulación a la baja de LEF1 podría explicar la resistencia al litio en las neuronas NR. Nuestros resultados muestran que el ácido valproico (VPA), un fármaco utilizado para tratar a pacientes con NR que también actúa aguas abajo de GSK-3β, reguló al alza los genes diana de LEF1 y Wnt/β-catenina, aumentó la actividad transcripcional del complejo β-catenina/TCF/LEF1 y redujo la excitabilidad en las neuronas NR. Además, la disminución de la expresión de LEF1 en las neuronas de control mediante shLEF1 provocó hiperexcitabilidad, lo que confirma que el impacto del VPA sobre la excitabilidad en las neuronas NR estaba relacionado con cambios en LEF1 y en la vía Wnt/β-catenina. Nuestros resultados sugieren que LEF1 podría ser una diana útil para el descubrimiento de nuevos fármacos para el tratamiento del trastorno bipolar.

Zholudeva, L.V., Abraira, V.E., Satkunendrarajah, K., McDevitt, T.C., Goulding, M.D., Magnuson, D.S.K., Lane, M.A. Spinal Interneurons as Gatekeepers to Neuroplasticity after Injury or Disease. (2021) Diario de Neurociencia. DOI: 10.1523/JNEUROSCI.1654-20.2020

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Trujillo, C.A., Adams, J.W., Negraes, P.D., Carromeu, C., Tejwani, L., Acab, A., Tsuda, B., Thomas, C.A., Sodhi, N., Fichter, K.M., Romero, S., Zanella, F., Sejnowski, T.J., Ulrich, H., Muotri, A.R. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. (2021) EMBO Molecular Medicine. 13(1):e12523. DOI: 10.15252/emmm.202012523

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Kim, R., Sejnowski, T.J. Strong inhibitory signaling underlies stable temporal dynamics and working memory in spiking neural networks. (2020) Nature Neuroscience. DOI: 10.1038/s41593-020-00753-w

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Tsuda, B., Tye, K.M., Siegelmann, H.T., Sejnowski, T.J. A modeling framework for adaptive lifelong learning with transfer and savings through gating in the prefrontal cortex. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.2009591117

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Peirs, C., Williams, S.G., Zhao, X., Arokiaraj, C.M., Ferreira, D.W., Noh, M.C., Smith, K.M., Halder, P., Corrigan, K.A., Gedeon, J.Y., Lee, S.J., Gatto, G., Chi, D., Ross, S.E., Goulding, M., Seal, R.P. Mechanical Allodynia Circuitry in the Dorsal Horn Is Defined by the Nature of the Injury. (2020) Neurona. DOI: 10.1016/j.neuron.2020.10.027

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Gatto, G., Bourane, S., Ren, X., Di Costanzo, S., Fenton, P.K., Halder, P., Seal, R.P., Goulding, M.D. A Functional Topographic Map for Spinal Sensorimotor Reflexes. (2020) Neurona. DOI: 10.1016/j.neuron.2020.10.003

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Wang, Q.W., Lu, S.Y., Liu, Y.N., Chen, Y., Wei, H., Shen, W., Chen, Y.F., Fu, C.L., Wang, Y.H., Dai, A., Huang, X., Gage, F.H., Xu, Q., Yao, J. La deficiencia de sinaptotagmina-7 induce anormalidades conductuales similares a la manía al atenuar la actividad de GluN2B. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.2016416117

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Roome, R.B., Bourojeni, F.B., Mona, B., Rastegar-Pouyani, S., Blain, R., Dumouchel, A., Salesse, C., Thompson, W.S., Brookbank, M., Gitton, Y., Tessarollo, L., Goulding, M., Johnson, J.E., Kmita, M., Chédotal, A., Kania, A. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans. (2020) Cell Reports. 33(8):108425. DOI: 10.1016/j.celrep.2020.108425

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Davis, W., Muller, L., Martinez-Trujillo, J., Sejnowski, T., Reynolds, J.H. Spontaneous travelling cortical waves gate perception in behaving primates (2020) Naturaleza. DOI: https://www.nature.com/articles/s41586-020-2802-y.

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Sejnowski, T. Charles Gross: Mentor, Maestro, Mensch. (2020) Progress in Neurobiology.:101926 DOI: 10.1016/j.pneurobio.2020.101926

Choi, S., Hachisuka, J., Brett, M.A., Magee, A.R., Omori, Y., Iqbal, N.U., Zhang, D., DeLisle, M.M., Wolfson, R.L., Bai, L., Santiago, C., Gong, S., Goulding, M., Heintz, N., Koerber, H.R., Ross, S.E., Ginty, D.D. Parallel ascending spinal pathways for affective touch and pain. [Pubmed] (2020) Naturaleza.:https://rdcu.be/b9ulT DOI: 10.1038/s41586-020-2860-1

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Linker, S.B., Randolph-Moore, L., Kottilil, K., Qiu, F., Jaeger, B.N., Barron, J., Gage, F.H. Identificación de la transcripción del retrotransposón B2 SINE `bona fide` mediante ARN-seq de núcleo único del hipocampo de ratón. (2020) Investigación del genoma. DOI: 10.1101/gr.262196.120

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Actualmente, los investigadores recurren a métodos generalizados para cuantificar la expresión del ARN de los elementos transponibles (TE), como la RT-qPCR y la secuenciación de ARN, que no distinguen entre los TE expresados a partir de su propio promotor (auténticos) y los TE que se transcriben desde el promotor de un gen vecino, como por ejemplo dentro de un intrón o un exón. Esta distinción es importante debido a las diferentes funciones que desempeñan los TE dependiendo de si se transcriben de forma independiente. Aquí presentamos una estrategia sencilla para examinar la expresión de TE genuinos, denominada BonaFide-TEseq. Este enfoque puede utilizarse con cualquier biblioteca basada en el cambio de molde, como Smart-seq2 o el kit de expresión génica 5' de célula única de 10x, lo que amplía su utilidad a la secuenciación de ARN de célula única. Este enfoque no requiere un enriquecimiento específico de TE, lo que permite el examen simultáneo de TE y genes codificadores de proteínas. Demostramos que los TE identificados mediante BonaFide-TEseq se expresan a partir de su propio promotor, en lugar de capturarse como productos internos de los genes. Revelamos la utilidad de BonaFide-TEseq en el análisis de datos de célula única y mostramos que los elementos nucleares intercalados cortos (SINE) presentan perfiles de expresión específicos del tipo celular en el hipocampo de ratón. Demostramos además que, en respuesta a una breve exposición de ratones en su jaula habitual a un estímulo novedoso, los SINE se activan en las neuronas granulares dentadas en un curso temporal similar al de los genes tempranos inmediatos que codifican proteínas. Este trabajo ofrece un enfoque alternativo sencillo para evaluar la transcripción genuina de los TE con resolución unicelular y proporciona una prueba de concepto utilizando este método para identificar la activación de los SINE en un contexto relevante para el aprendizaje y la memoria normales.

Gonzalez, C.E., Lainscsek, C., Sejnowski, T.J., Letellier, C. Assessing observability of chaotic systems using Delay Differential Analysis. (2020) Chaos. 30(10):103113. DOI: 10.1063/5.0015533

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Sobczak, F., He, Y., Sejnowski, T.J., Yu, X. Predicting the fMRI Signal Fluctuation with Recurrent Neural Networks Trained on Vascular Network Dynamics. (2020) Cerebral Cortex . DOI: 10.1093/cercor/bhaa260

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Abbott, L.F., Bock, D.D., Callaway, E.M., Denk, W., Dulac, C., Fairhall, A.L., Fiete, I., Harris, K.M., Helmstaedter, M., Jain, V., Kasthuri, N., LeCun, Y., Lichtman, J.W., Littlewood, P.B., Luo, L., Maunsell, J.H.R., Reid, R.C., Rosen, B.R., Rubin, G.M., Sejnowski, T.J., Seung, H.S., Svoboda, K., Tank, D.W., Tsao, D., Van Essen, D.C. The Mind of a Mouse. (2020) Móvil. 182(6):1372-1376. DOI: 10.1016/j.cell.2020.08.010

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Lin, T.W., Chen, Y., Bukhari, Q., Krishnan, G.P., Bazhenov, M., Sejnowski, T.J. Differential Covariance: A New Method to Estimate Functional Connectivity in fMRI. (2020) Computación neuronal.:1-33 DOI: 10.1162/neco_a_01323

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Yang, C.F., Kim, E.J., Callaway, E.M., Feldman, J.L. Monosynaptic Projections to Excitatory and Inhibitory preBötzinger Complex Neurons. (2020) Fronteras en Neuroanatomía. 14:58. DOI: 10.3389/fnana.2020.00058

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Figueiredo, T., Mendes, A.P.D., Moreira, D.P., Goulart, E., Oliveira, D., Kobayashi, G.S., Stern, S., Kok, F., Marchetto, M.C., Santos, R., Gage, F.H., Zatz, M. La mutación en la inositol monofosfatasa 1 (IMPA1) en pacientes con discapacidad intelectual afecta la neurogénesis, pero no la gliogénesis. (2020) Psiquiatría Molecular. DOI: 10.1038/s41380-020-00862-9

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Recientemente se identificó una mutación homocigótica en el gen de la inositol monofosfatasa 1 (IMPA1) en nueve personas con discapacidad intelectual (DI) grave y comportamiento disruptivo. Estas personas pertenecen a la misma familia del noreste de Brasil, que cuenta con 28 matrimonios consanguíneos y 59 miembros de la familia genotipados. El IMPA1 es responsable de la generación de inositol libre a partir de la biosíntesis de novo y del reciclaje de polifosfatos de inositol, y participa en la vía de señalización del fosfatidilinositol. Para comprender el papel de la deficiencia de IMPA1 en la DI, generamos células madre pluripotentes inducidas (iPSC) a partir de pacientes y controles neurotípicos, y las diferenciamos en neuronas similares al giro dentado del hipocampo y astrocitos. Las células progenitoras neuronales (NPC) con deficiencia de IMPA1 revelaron déficits sustanciales en la proliferación y el potencial neurogénico. En NPC de bajo paso (P1 a P3), observamos detención del ciclo celular, apoptosis, cambio progresivo a una morfología glial y reducción de la diferenciación neuronal. Estas observaciones se validaron mediante la recuperación del fenotipo con medios suplementados con mio-inositol durante la diferenciación de las iPSC derivadas de pacientes en neuronas y mediante la reducción del potencial neurogénico en las NPC de control que expresaban shIMPA1. El análisis del transcriptoma mostró que las NPC y las neuronas derivadas de pacientes con ID presentan una desregulación extensa de la expresión génica que afecta a las vías necesarias para la neurogénesis y la regulación al alza de genes gliogénicos. La deficiencia de IMPA1 no afectó la progresión del ciclo celular ni la supervivencia en las iPSC y las células progenitoras gliales, ni la diferenciación de astrocitos. Por lo tanto, este estudio demuestra que la mutación de IMPA1 afecta específicamente la supervivencia de las NPC y la diferenciación neuronal.

Kerman, B.E., Genoud, S., Kurt Vatandaslar, B., Denli, A.M., Ghosh, S.G., Xu, X., Yeo, G.W., Aimone, J.B., Gage, F.H. El perfil de expresión de motoneuronas identifica una asociación entre una variante de empalme axonal de la proteína 3 relacionada con HDGF y la mielinización periférica. (2020) Revista de Química Biológica. DOI: 10.1074/jbc.RA120.014329

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Los trastornos que alteran la formación de la mielina durante el desarrollo o en la edad adulta, como la esclerosis múltiple y las neuropatías periféricas, dan lugar a patologías graves, lo que pone de manifiesto el papel crucial de la mielina en el funcionamiento neuronal normal. Sin embargo, aunque nuestra comprensión de la biología glial está aumentando, las señales que emanan de los axones y regulan la mielinización siguen siendo en gran parte desconocidas. Para identificar los componentes centrales del proceso de mielinización, en este estudio adoptamos un enfoque de análisis de microarrays combinado con microdisección por captura láser de motoneuronas espinales durante la fase mielinogénica del desarrollo. Identificamos genes neuronales cuya expresión se enriquecía durante la mielinización e investigamos más a fondo la proteína 3 relacionada con el factor de crecimiento derivado del hepatoma (HRP3 o HDGFRP3). La HRP3 se expresó intensamente en los tractos de fibras de la materia blanca de los sistemas nerviosos periférico (SNP) y central (SNC) durante la mielinización y la remielinización en un modelo de desmielinización inducida por cuprizona. La localización dinámica de HRP3 entre los axones y los núcleos durante la mielinización fue consistente con su localización axonal durante la neuritogénesis. Para estudiar este fenómeno, identificamos dos variantes de empalme codificadas por el gen: la isoforma canónica HRP3-I y una isoforma recientemente reconocida, HRP3-II. HRP3-I permaneció exclusivamente en el núcleo, mientras que HRP3-II mostró una localización axonal distinta tanto antes como durante la mielinización. Curiosamente, HRP3-II permaneció en los núcleos de las neuronas y células gliales no mielinizadas, lo que sugiere la existencia de un mecanismo molecular que lo transfiere y lo retiene en los axones de las neuronas destinadas a la mielinización. La sobreexpresión de HRP3-II, pero no de HRP3-I, aumentó el número de células de Schwann y la mielinización en los cocultivos de neuronas y glía del SNP. Sin embargo, la sobreexpresión de HRP3-II en los cocultivos del SNC no alteró la mielinización.

Ordyan, M., Bartol, T., Kennedy, M., Rangamani, P., Sejnowski, T. Interactions between calmodulin and neurogranin govern the dynamics of CaMKII as a leaky integrator. (2020) PLOS Biología Computacional. 16(7):e1008015. DOI: 10.1371/journal.pcbi.1008015

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Calmodulin-dependent kinase II (CaMKII) has long been known to play an important role in learning and memory as well as long term potentiation (LTP). More recently it has been suggested that it might be involved in the time averaging of synaptic signals, which can then lead to the high precision of information stored at a single synapse. However, the role of the scaffolding molecule, neurogranin (Ng), in governing the dynamics of CaMKII is not yet fully understood. In this work, we adopt a rule-based modeling approach through the Monte Carlo method to study the effect of Ca2+ signals on the dynamics of CaMKII phosphorylation in the postsynaptic density (PSD). Calcium surges are observed in synaptic spines during an EPSP and back-propagating action potential due to the opening of NMDA receptors and voltage dependent calcium channels. Using agent-based models, we computationally investigate the dynamics of phosphorylation of CaMKII monomers and dodecameric holoenzymes. The scaffolding molecule, Ng, when present in significant concentration, limits the availability of free calmodulin (CaM), the protein which activates CaMKII in the presence of calcium. We show that Ng plays an important modulatory role in CaMKII phosphorylation following a surge of high calcium concentration. We find a non-intuitive dependence of this effect on CaM concentration that results from the different affinities of CaM for CaMKII depending on the number of calcium ions bound to the former. It has been shown previously that in the absence of phosphatase, CaMKII monomers integrate over Ca2+ signals of certain frequencies through autophosphorylation (Pepke et al, Plos Comp. Bio., 2010). We also study the effect of multiple calcium spikes on CaMKII holoenzyme autophosphorylation, and show that in the presence of phosphatase, CaMKII behaves as a leaky integrator of calcium signals, a result that has been recently observed in vivo. Our models predict that the parameters of this leaky integrator are finely tuned through the interactions of Ng, CaM, CaMKII, and PP1, providing a mechanism to precisely control the sensitivity of synapses to calcium signals. Author Summary not valid for PLOS ONE submissions.

Ceto, S., Sekiguchi, K.J., Takashima, Y., Nimmerjahn, A., Tuszynski, M.H. Neural Stem Cell Grafts Form Extensive Synaptic Networks that Integrate with Host Circuits after Spinal Cord Injury. (2020) Células Madre. DOI: 10.1016/j.stem.2020.07.007

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Goncalves, J., Bartol, T.M., Camus, C., Levet, F., Menegolla, A.P., Sejnowski, T.J., Sibarita, J.B., Vivaudou, M., Choquet, D., Hosy, E. Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1922563117

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Mansour, A.A., Schafer, S.T., Gage, F.H. Complejidad celular en organoides cerebrales: progreso actual y problemas no resueltos. (2020) Seminarios en Biología Celular y del Desarrollo. DOI: 10.1016/j.semcdb.2020.05.013

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Filimon, F., Nelson, J.D., Sejnowski, T.J., Sereno, M.I., Cottrell, G.W. The ventral striatum dissociates information expectation, reward anticipation, and reward receipt. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1911778117

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Kim, E.J., Zhang, Z., Huang, L., Ito-Cole, T., Jacobs, M.W., Juavinett, A.L., Senturk, G., Hu, M., Ku, M., Ecker, J.R., Callaway, E.M. Extraction of Distinct Neuronal Cell Types from within a Genetically Continuous Population. (2020) Neurona. DOI: 10.1016/j.neuron.2020.04.018

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Kirchgessner, M.A., Franklin, A.D., Callaway, E.M. Context-dependent and dynamic functional influence of corticothalamic pathways to first- and higher-order visual thalamus. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.2002080117

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Wenderski, W., Wang, L., Krokhotin, A., Walsh, J.J., Li, H., Shoji, H., Ghosh, S., George, R.D., Miller, E.L., Elias, L., Gillespie, M.A., Son, E.Y., Staahl, B.T., Baek, S.T., Stanley, V., Moncada, C., Shipony, Z., Linker, S.B., Marchetto, M.C.N., Gage, F.H., Chen, D., Sultan, T., Zaki, M.S., Ranish, J.A., Miyakawa, T., Luo, L., Malenka, R.C., Crabtree, G.R., Gleeson, J.G. La pérdida de la subunidad BAF específica neuronal ACTL6B alivia la represión de genes de respuesta temprana y causa autismo recesivo. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. [DOI: 10.1073/pnas.1908238117]

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La actividad sináptica en las neuronas provoca la rápida activación de genes implicados en el comportamiento de los mamíferos. Los remodeladores de la cromatina dependientes del ATP, como el complejo BAF, contribuyen a estas respuestas y, en general, se cree que activan la transcripción. Sin embargo, los mecanismos que mantienen inactivos esos genes de "activación temprana" han sido un misterio. En el curso de la investigación del autismo recesivo mendeliano, identificamos seis familias con mutaciones segregantes de pérdida de función en la subunidad neuronal del BAF (nBAF) (originalmente denominada ). En consecuencia, fue el gen con mutaciones más significativas en la Cohorte de Autismo Recesivo de Simons. Al menos 14 subunidades del complejo nBAF presentan mutaciones en el autismo, lo que en conjunto lo convierte en un factor importante en el trastorno del espectro autista (TEA). Las mutaciones de los pacientes desestabilizaron la proteína ACTL6B en las neuronas y desviaron las dendritas hacia el glomérulo incorrecto en el sistema olfativo de la mosca. Los seres humanos y los ratones que carecían de presentaban hipoplasia del cuerpo calloso, lo que indica un papel conservado de en la facilitación de la conectividad neural. Los ratones knockout en dos fondos genéticos exhibieron comportamientos relacionados con el TEA, incluyendo deficiencias sociales y de memoria, comportamientos repetitivos e hiperactividad. Sorprendentemente, la mutación de aliviaba la represión de genes de respuesta temprana, incluidos los factores de transcripción AP1 (, , , y ), aumentaba la accesibilidad de la cromatina en los sitios de unión de AP1 y provocaba cambios transcripcionales en genes de respuesta tardía asociados con la actividad de los factores de transcripción de respuesta temprana. La pérdida de es, por lo tanto, una causa importante del TEA recesivo, y la represión de la cromatina específica de las neuronas alterada se indica como un posible mecanismo.

Sun, G., Chiuppesi, F., Chen, X., Wang, C., Tian, E., Nguyen, J., Kha, M., Trinh, D., Zhang, H., Marchetto, M.C., Song, H., Ming, G.L., Gage, F.H., Diamond, D.J., Wussow, F., Shi, Y. Modelado de la microcefalia inducida por el citomegalovirus humano en organoides cerebrales derivados de iPSC humanas. (2020) Cell Reports Medicine. 1(1):100002. DOI: 10.1016/j.xcrm.2020.100002

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DAS, A., Cash, S.S., Sejnowski, T.J. Heterogeneity of Preictal Dynamics in Human Epileptic Seizures. (2020) IEEE Access. 8:52738-52748. DOI: 10.1109/access.2020.2981017

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It is generally understood that there is a preictal phase in the development of a seizure and this precictal period is the basis for seizure prediction attempts. The focus of this study is the preictal global spatiotemporal dynamics and its intra-patient variability. We analyzed preictal broadband brain connectivity from human electrocorticography (ECoG) recordings of 185 seizures (which included 116 clinical seizures) collected from 12 patients. ECoG electrodes record from only a part of the cortex, leaving large regions of the brain unobserved. Brain connectivity was therefore estimated using the sparse-plus-latent-regularized precision matrix (SLRPM) method, which calculates connectivity from partial correlations of the conditional statistics of the observed regions given the unobserved latent regions. Brain connectivity was quantified using eigenvector centrality (EC), from which a degree of heterogeneity was calculated for the preictal periods of all seizures in each patient. Results from the SLRPM method are compared to those from the sparse-regularized precision matrix (SRPM) and correlation methods, which do not account for the unobserved inputs when estimating brain connectivity. The degree of heterogeneity estimated by the SLRPM method is higher than those estimated by the SRPM and correlation methods for the preictal periods in most patients. These results reveal substantial heterogeneity or desynchronization among brain areas in the preictal period of human epileptic seizures. Furthermore, the SLRPM method identifies more onset channels from the preictal active electrodes compared to the SRPM and correlation methods. Finally, the correlation between the degree of heterogeneity and seizure severity of patients for SLRPM and SRPM methods were lower than that obtained from the correlation method. These results support recent findings suggesting that inhibitory neurons can have anti-seizure effects by inducing variability or heterogeneity across seizures. Understanding how this variability is linked to seizure initiation may lead to better predictions and controlling therapies.

Shen, W., Wang, Q.W., Liu, Y.N., Marchetto, M.C., Linker, S., Lu, S.Y., Chen, Y., Liu, C., Guo, C., Xing, Z., Shi, W., Kelsoe, J.R., Alda, M., Wang, H., Zhong, Y., Sui, S.F., Zhao, M., Yang, Y., Mi, S., Cao, L., Gage, F.H., Yao, J. La sinaptotagmina-7 es un factor clave para las anomalías conductuales tipo bipolar en ratones. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1918165117

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La patogénesis del trastorno bipolar (TB) sigue siendo un misterio, en gran parte porque los modelos animales genéticos basados en genes de susceptibilidad identificados a menudo no han logrado mostrar los síntomas centrales de los ciclos espontáneos del estado de ánimo. Sin embargo, los análisis basados en pedigríes y en células madre pluripotentes inducidas (iPSC) han sugerido que la disfunción en algunas cascadas de señalización clave podría ser crucial para la patogénesis de la enfermedad en una subpoblación de pacientes con TB. Planteamos la hipótesis de que las anomalías conductuales de los pacientes y las anomalías metabólicas comórbidas podrían compartir algún mecanismo molecular idéntico. Por lo tanto, investigamos la expresión de genes con doble función (insulina/sinapsis) en neuronas derivadas de las iPSC de pacientes con TB y el fenotipo conductual de ratones con estos genes silenciados en el hipocampo. De esta manera, identificamos la sinaptotagmina-7 (Syt7) como un factor de riesgo candidato para las anomalías conductuales. A continuación, investigamos ratones knockout (KO) para Syt7 y observamos fluctuaciones conductuales nocturnas de tipo maníaco y diurnas de tipo depresivo en la mayoría de estos animales, análogas a los síntomas de ciclos del estado de ánimo del TB. Tratamos a los ratones KO para Syt7 con fármacos clínicos para el TB, incluyendo olanzapina y litio, y descubrimos que los tratamientos farmacológicos podían regular eficazmente las anomalías conductuales de los ratones KO para Syt7. Para verificar aún más si existían déficits de Syt7 en pacientes con TB, investigamos las muestras de plasma de 20 pacientes con TB y descubrimos que el nivel de ARNm de Syt7 estaba significativamente atenuado en el plasma de los pacientes en comparación con los controles sanos. Por lo tanto, concluimos que Syt7 es probablemente un factor clave para las anomalías conductuales de tipo bipolar.

Stern, S., Sarkar, A., Galor, D., Stern, T., Mei, A., Stern, Y., Mendes, A.P.D., Randolph-Moore, L., Rouleau, G., Bang, A.G., Santos, R., Alda, M., Marchetto, M.C., Gage, F.H. Una inestabilidad fisiológica mostrada en neuronas del hipocampo derivadas de pacientes con trastorno bipolar no respondedores a litio. (2020) Psiquiatría Biológica. DOI: 10.1016/j.biopsych.2020.01.020

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Sejnowski, T.J. The unreasonable effectiveness of deep learning in artificial intelligence. (2020) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1907373117

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Wang, M., Wei, P.C., Lim, C.K., Gallina, I.S., Marshall, S., Marchetto, M.C., Alt, F.W., Gage, F.H. Proliferación incrementada de progenitores neurales en un modelo de autismo con iPSC induce inestabilidad genómica asociada a estrés de replicación. (2020) Células Madre. 26(2):221-233.e6 . DOI: 10.1016/j.stem.2019.12.013

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Nimmerjahn, A. Monitoring neuronal health. (2020) Ciencia. 367(6477):510-511. DOI: 10.1126/science.aba4472

Fischer, K.B., Collins, H.K., Callaway, E.M. Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. (2019) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 116(52):27001–27010. DOI: 10.1073/pnas.1915974116

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Marsala, M., Kamizato, K., Tadokoro, T., Navarro, M., Juhas, S., Juhasova, J., Marsala, S., Studenovska, H., Proks, V., Hazel, T., Johe, K., Kakinohana, M., Driscoll, S., Thomas, G., Pfaff, S., Ciacci, J. Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats. (2019) Stem Cells Translational Medicine. DOI: 10.1002/sctm.19-0156

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Garcia, G.C., Bartol, T.M., Phan, S., Bushong, E.A., Perkins, G., Sejnowski, T.J., Ellisman, M.H., Skupin, A. Mitochondrial morphology provides a mechanism for energy buffering at synapses. (2019) Scientific Reports. 9(1):18306. DOI: 10.1038/s41598-019-54159-1

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Jullié, D., Stoeber, M., Sibarita, J.B., Zieger, H.L., Bartol, T.M., Arttamangkul, S., Sejnowski, T.J., Hosy, E., von Zastrow, M. A Discrete Presynaptic Vesicle Cycle for Neuromodulator Receptors. (2019) Neurona. DOI: 10.1016/j.neuron.2019.11.016

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Reichenstein, I., Eitan, C., Diaz-Garcia, S., Haim, G., Magen, I., Siany, A., Hoye, M.L., Rivkin, N., Olender, T., Toth, B., Ravid, R., Mandelbaum, A.D., Yanowski, E., Liang, J., Rymer, J.K., Levy, R., Beck, G., Ainbinder, E., Farhan, S.M.K., Lennox, K.A., Bode, N.M., Behlke, M.A., Möller, T., Saxena, S., Moreno, C.A.M., Costaguta, G., van Eijk, K.R., Phatnani, H., Al-Chalabi, A., Başak, A.N., van den Berg, L.H., Hardiman, O., Landers, J.E., Mora, J.S., Morrison, K.E., Shaw, P.J., Veldink, J.H., Pfaff, S.L., Yizhar, O., Gross, C., Brown, R.H., Ravits, J.M., Harms, M.B., Miller, T.M., Hornstein, E. Human genetics and neuropathology suggest a link between miR-218 and amyotrophic lateral sclerosis pathophysiology. (2019) Medicina Traslacional en Ciencia. 11(523). DOI: 10.1126/scitranslmed.aav5264

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Pharris, M.C., Patel, N.M., VanDyk, T.G., Bartol, T.M., Sejnowski, T.J., Kennedy, M.B., Stefan, M.I., Kinzer-Ursem, T.L. A Multi-State Model of the CaMKII Dodecamer Suggests a Role for Calmodulin in Maintenance of Autophosphorylation. (2019) PLOS Biología Computacional. 15(12):e1006941. DOI: 10.1371/journal.pcbi.1006941

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Bravo-Hernandez, M., Tadokoro, T., Navarro, M.R., Platoshyn, O., Kobayashi, Y., Marsala, S., Miyanohara, A., Juhas, S., Juhasova, J., Skalnikova, H., Tomori, Z., Vanicky, I., Studenovska, H., Proks, V., Chen, P., Govea-Perez, N., Ditsworth, D., Ciacci, J.D., Gao, S., Zhu, W., Ahrens, E.T., Driscoll, S.P., Glenn, T.D., McAlonis-Downes, M., Da Cruz, S., Pfaff, S.L., Kaspar, B.K., Cleveland, D.W., Marsala, M. Spinal subpial delivery of AAV9 enables widespread gene silencing and blocks motoneuron degeneration in ALS. (2019) Medicina Natural. DOI: 10.1038/s41591-019-0674-1

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Lainscsek, C., Rungratsameetaweemana, N., Cash, S.S., Sejnowski, T.J. Cortical chimera states predict epileptic seizures. (2019) Chaos. 29(12):121106. DOI: 10.1063/1.5139654

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Ruiz-Martínez, , Bartol, T.M., Sejnowski, T.J., Tartakovsky, D.M. Stochastic self-tuning hybrid algorithm for reaction-diffusion systems. (2019) Journal of Chemical Physics. 151(24):244117. DOI: 10.1063/1.5125022

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Zhu, Z., Mesci, P., Bernatchez, J.A., Gimple, R.C., Wang, X., Schafer, S.T., Wettersten, H.I., Beck, S., Clark, A.E., Wu, Q., Prager, B.C., Kim, L.J.Y., Dhanwani, R., Sharma, S., Garancher, A., Weis, S.M., Mack, S.C., Negraes, P.D., Trujillo, C.A., Penalva, L.O., Feng, J., Lan, Z., Zhang, R., Wessel, A.W., Dhawan, S., Diamond, M.S., Chen, C.C., Wechsler-Reya, R.J., Gage, F.H., Hu, H., Siqueira-Neto, J.L., Muotri, A.R., Cheresh, D.A., Rich, J.N. El virus Zika se dirige a las células madre del glioblastoma a través de un eje SOX2-integrina αβ. (2019) Células Madre. 26(2):187-204. DOI: 10.1016/j.stem.2019.11.016

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Siddharth, S., Jung, T.P., Sejnowski, T.J. Impact of Affective Multimedia Content on the Electroencephalogram and Facial Expressions. (2019) Scientific Reports. 9(1):16295. DOI: 10.1038/s41598-019-52891-2

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De la Fuente, I.M., Bringas, C., Malaina, I., Regner, B., Pérez-Samartín, A., Boyano, M.D., Fedetz, M., López, J.I., Pérez-Yarza, G., Cortes, J.M., Sejnowski, T. The nucleus does not significantly affect the migratory trajectories of amoeba in two-dimensional environments. (2019) Scientific Reports. 9(1):16369. DOI: 10.1038/s41598-019-52716-2

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Nott, A., Holtman, I.R., Coufal, N.G., Schlachetzki, J.C.M., Yu, M., Hu, R., Han, C.Z., Pena, M., Xiao, J., Wu, Y., Keulen, Z., Pasillas, M.P., O'Connor, C., Nickl, C.K., Schafer, S.T., Shen, Z., Rissman, R.A., Brewer, J.B., Gosselin, D., Gonda, D.D., Levy, M.L., Rosenfeld, M.G., McVicker, G., Gage, F.H., Ren, B., Glass, C.K. Mapas de interactomas promotor-potenciador específicos de tipos de células cerebrales y asociación de riesgo de enfermedad. (2019) Ciencia. 366(6469):1134-1139. DOI: 10.1126/science.aay0793

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Patriarchi, T., Cho, J.R., Merten, K., Marley, A., Broussard, G.J., Liang, R., Williams, J., Nimmerjahn, A., von Zastrow, M., Gradinaru, V., Tian, L. Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators. (2019) Nature Protocols. 14(12):3471–3505. DOI: 10.1038/s41596-019-0239-2

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Vadodaria, K.C., Jones, J.R., Linker, S., Gage, F.H. Modelado de Trastornos Cerebrales Usando Células Madre Pluripotentes Inducidas. (2019) Perspectivas de Cold Spring Harbor en Biología. DOI: 10.1101/cshperspect.a035659

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Sharpee, T.O., Berkowitz, J.A. Linking neural responses to behavior with information-preserving population vectors (2019) Current Opinion in Behavioral Sciences. 29:37-44. DOI: https://doi.org/10.1016/j.cobeha.2019.03.004

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Litvina, E., Adams, A., Barth, A., Bruchez, M., Carson, J., Chung, J.E., Dupre, K.B., Frank, L.M., Gates, K.M., Harris, K.M., Joo, H., William Lichtman, J., Ramos, K.M., Sejnowski, T., Trimmer, J.S., White, S., Koroshetz, W. BRAIN Initiative: Cutting-Edge Tools and Resources for the Community. (2019) Diario de Neurociencia. 39(42):8275-8284. DOI: 10.1523/JNEUROSCI.1169-19.2019

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The overarching goal of the NIH BRAIN (Brain Research through Advancing Innovative Neurotechnologies) Initiative is to advance the understanding of healthy and diseased brain circuit function through technological innovation. Core principles for this goal include the validation and dissemination of the myriad innovative technologies, tools, methods, and resources emerging from BRAIN-funded research. Innovators, BRAIN funding agencies, and non-Federal partners are working together to develop strategies for making these products usable, available, and accessible to the scientific community. Here, we describe several early strategies for supporting the dissemination of BRAIN technologies. We aim to invigorate a dialogue with the neuroscience research and funding community, interdisciplinary collaborators, and trainees about the existing and future opportunities for cultivating groundbreaking research products into mature, integrated, and adaptable research systems. Along with the accompanying Society for Neuroscience 2019 Mini-Symposium, "BRAIN Initiative: Cutting-Edge Tools and Resources for the Community," we spotlight the work of several BRAIN investigator teams who are making progress toward providing tools, technologies, and services for the neuroscience community. These tools access neural circuits at multiple levels of analysis, from subcellular composition to brain-wide network connectivity, including the following: integrated systems for EM- and florescence-based connectomics, advances in immunolabeling capabilities, and resources for recording and analyzing functional connectivity. Investigators describe how the resources they provide to the community will contribute to achieving the goals of the NIH BRAIN Initiative. Finally, in addition to celebrating the contributions of these BRAIN-funded investigators, the Mini-Symposium will illustrate the broader diversity of BRAIN Initiative investments in cutting-edge technologies and resources.

Kim, R., Li, Y., Sejnowski, T.J. Simple framework for constructing functional spiking recurrent neural networks. (2019) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1905926116

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Lainscsek, C., Gonzalez, C.E., Sampson, A.L., Cash, S.S., Sejnowski, T.J. Causality detection in cortical seizure dynamics using cross-dynamical delay differential analysis. (2019) Chaos. 29(10):101103. DOI: 10.1063/1.5126125

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Stern, S., Sarkar, A., Stern, T., Mei, A., Mendes, A.P.D., Stern, Y., Goldberg, G., Galor, D., Nguyen, T., Randolph-Moore, L., Kim, Y., Rouleau, G., Bang, A., Alda, M., Santos, R., Marchetto, M.C., Gage, F.H. Mecanismos subyacentes a la hiperexcitabilidad de las neuronas del hipocampo CA3 y del giro dentado derivadas de pacientes con trastorno bipolar. (2019) Psiquiatría Biológica. DOI: 10.1016/j.biopsych.2019.09.018

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Paixão, S., Loschek, L., Gaitanos, L., Alcalà Morales, P., Goulding, M., Klein, R. Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements. (2019) Neurona. 104(4):749-764. DOI: 10.1016/j.neuron.2019.08.029

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Hafner, G., Witte, M., Guy, J., Subhashini, N., Fenno, L.E., Ramakrishnan, C., Kim, Y.S., Deisseroth, K., Callaway, E.M., Oberhuber, M., Conzelmann, K.K., Staiger, J.F. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. (2019) Cell Reports. 28(13):3450-3461.e8. DOI: 10.1016/j.celrep.2019.08.064

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Cugno, A., Bartol, T.M., Sejnowski, T.J., Iyengar, R., Rangamani, P. Geometric principles of second messenger dynamics in dendritic spines. (2019) Scientific Reports. 9(1):11676. DOI: 10.1038/s41598-019-48028-0

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Amatya, D.N., Linker, S.B., Mendes, A.P.D., Santos, R., Erikson, G., Shokhirev, M.N., Zhou, Y., Sharpee, T., Gage, F.H., Marchetto, M.C., Kim, Y. La complejidad eléctrica dinámica se reduce durante la diferenciación neuronal en el trastorno del espectro autista. (2019) Stem Cell Reports. DOI: 10.1016/j.stemcr.2019.08.001

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Sharpee, T.O. An argument for hyperbolic geometry in neural circuits. (2019) Current Opinion in Neurobiology. 58:101-104. DOI: 10.1016/j.conb.2019.07.008

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Juavinett, A.L., Kim, E.J., Collins, H.C., Callaway, E.M. A systematic topographical relationship between mouse lateral posterior thalamic neurons and their visual cortical projection targets. (2019) Journal of Comparative Neurology. 528(1):95-107. DOI: 10.1002/cne.24737

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Wang, H.P., Garcia, J.W., Sabottke, C.F., Spencer, D., Sejnowski, T.J. Feedforward thalamocortical connectivity preserves stimulus timing information in sensory pathways. (2019) Diario de Neurociencia. DOI: 10.1523/JNEUROSCI.3165-17.2019

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Reliable timing of cortical spikes in response to visual events is crucial in representing visual inputs to the brain. Spikes in the primary visual cortex need to occur at the same time within a repeated visual stimulus. Two classical mechanisms are employed by the cortex to enhance reliable timing. First, cortical neurons respond reliably to a restricted set of stimuli through their preference for certain patterns of membrane potential due to their intrinsic properties. Second, intracortical networking of excitatory and inhibitory neurons induces lateral inhibition that, through the timing and strength of IPSCs and EPSCs, produces sparse and reliably timed cortical neuron spike trains to be transmitted downstream. Here we describe a third mechanism that, through preferential thalamocortical synaptic connectivity, enhances the trial-to-trial timing precision of cortical spikes in the presence of spike train variability within each trial that is introduced between LGN neurons in the retino-thalamic pathway. Applying experimentally recorded LGN spike trains from the anesthetized cat to a detailed model of a spiny stellate V1 neuron, we found that output spike timing precision improved with increasing numbers of convergent LGN inputs. The improvement was consistent with the predicted proportionality of √???? for ???? LGN source neurons. We also found connectivity configurations that maximize reliability and that generate V1 cell output spike trains quantitatively similar to the experimental recordings. Our findings suggest a general principle, namely intra-trial variability among converging inputs, that increases stimulus response precision and is widely applicable to synaptically connected spiking neurons.The early visual pathway of the cat is favorable for studying the effects of trial-to-trial variability of synaptic inputs and intra-trial variability of thalamocortical connectivity on information transmission into the visual cortex. We have used a detailed model to show that there are preferred combinations of the number of thalamic afferents and the number of synapses per afferent that maximize the output reliability and spike-timing precision of cortical neurons. This provides additional insights into how synchrony in thalamic spike trains can reduce trial-to-trial variability to produce highly reliable reporting of sensory events to the cortex. The same principles may apply to other converging pathways where temporally jittered spike trains can reliably drive the downstream neuron and improve temporal precision.

Acton, D., Ren, X., Di Costanzo, S., Dalet, A., Bourane, S., Bertocchi, I., Eva, C., Goulding, M. Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch. (2019) Cell Reports. 28(3):625-639.e6. DOI: 10.1016/j.celrep.2019.06.033

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Bell, M., Bartol, T., Sejnowski, T., Rangamani, P. Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium. (2019) J. Gen. Physiol. DOI: 10.1085/jgp.201812261

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Quraishi, I.H., Stern, S., Mangan, K.P., Zhang, Y., Ali, S.R., Mercier, M.R., Marchetto, M.C., McLachlan, M.J., Jones, E.M., Gage, F.H., Kaczmarek, L.K. Una mutación KCNT1 asociada a la epilepsia aumenta la excitabilidad de neuronas derivadas de iPSC humanas mediante el aumento de las corrientes de potasio Slack. (2019) Diario de Neurociencia. DOI: 10.1523/JNEUROSCI.1628-18.2019

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Las mutaciones en el canal de potasio activado por sodio KCNT1 (Slack, K1.1) provocan encefalopatías epilépticas graves. La expresión en sistemas heterólogos ha demostrado que las mutaciones causantes de la enfermedad dan lugar a canales con una mayor amplitud de corriente. Sin embargo, se desconoce si dicha ganancia de función se produce en las neuronas humanas, ni si cabe esperar que ese aumento de la corriente de potasio suprima o aumente la excitabilidad de las neuronas corticales. Utilizando neuronas derivadas de células madre pluripotentes inducidas (iPSC) humanas modificadas genéticamente, hemos descubierto ahora que las corrientes de potasio dependientes del sodio se multiplican varias veces en las neuronas que portan una mutación homocigótica P924L. En registros de corriente de fijación, el aumento de la corriente de potasio en las neuronas con la mutación P924L actúa acortando la duración de los potenciales de acción y aumentando la amplitud de la poshiperpolarización que sigue a cada potencial de acción. Sorprendentemente, el número de potenciales de acción evocados por corrientes despolarizantes, así como las tasas máximas de disparo, aumentaron en las neuronas que expresaban el canal mutante. En redes de neuronas espontáneamente activas, la tasa media de disparo, la aparición de ráfagas rápidas de potenciales de acción y la intensidad del disparo durante la ráfaga aumentaron en las neuronas con la mutación P924L de Slack. La viabilidad de que un aumento de la corriente K aumente las tasas de disparo independientemente de cualquier cambio compensatorio se validó mediante simulaciones numéricas. Nuestros hallazgos indican que la ganancia de función en los canales K Slack causa hiperexcitabilidad tanto en neuronas aisladas como en redes neuronales y se produce mediante un mecanismo autónomo de la célula que no requiere interacciones de red. Las mutaciones del KCNT1 conducen a encefalopatías epilépticas graves para las que no existen tratamientos eficaces. Este estudio es la primera demostración de que una mutación en KCNT1 aumenta la corriente Slack en las neuronas. También ofrece la primera explicación de cómo este aumento de la corriente de potasio induce hiperexcitabilidad, lo que podría ser el factor subyacente que causa las convulsiones.

Pinto-Duarte, A., Roberts, A.J., Ouyang, K., Sejnowski, T.J. Impairments in remote memory caused by the lack of Type 2 IP receptors. (2019) Glia. DOI: 10.1002/glia.23679

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Stern, S., Linker, S., Vadodaria, K.C., Marchetto, M.C., Gage, F.H. Predicción de la respuesta a la terapia farmacológica en trastornos psiquiátricos. (2019) Focus (American Psychiatric Publishing). 17(3):294-307. DOI: 10.1176/appi.focus.17304

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Vadodaria, K.C., Ji, Y., Skime, M., Paquola, A., Nelson, T., Hall-Flavin, D., Fredlender, C., Heard, K.J., Deng, Y., Le, A.T., Dave, S., Fung, L., Li, X., Marchetto, M.C., Weinshilboum, R., Gage, F.H. Estudio de la resistencia al tratamiento de la depresión mediante el uso in vitro de neuronas derivadas de pacientes. (2019) Psiquiatría Molecular. 24(6):775. DOI: 10.1038/s41380-019-0424-2

Sommer, A., Marxreiter, F., Krach, F., Fadler, T., Grosch, J., Maroni, M., Graef, D., Eberhardt, E., Riemenschneider, M.J., Yeo, G.W., Kohl, Z., Xiang, W., Gage, F.H., Winkler, J., Prots, I., Winner, B. Linfocitos Th17 Inducen Muerte Neuronal en un Modelo Basado en iPSC Humanas de la Enfermedad de Parkinson. (2019) Células Madre. 24(6):1006. DOI: 10.1016/j.stem.2019.04.019

Nelson, N.A., Wang, X., Cook, D., Carey, E.M., Nimmerjahn, A. Imaging spinal cord activity in behaving animals. (2019) Experimental Neurology. 230:112974. DOI: 10.1016/j.expneurol.2019.112974

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Zhou, J., Ma, J., Chen, Y., Cheng, C., Bao, B., Peng, J., Sejnowski, T.J., Dixon, J.R., Ecker, J.R. Robust single-cell Hi-C clustering by convolution- and random-walk-based imputation. (2019) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1901423116

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Oakley, B.A., Sejnowski, T.J. What we learned from creating one of the world's most popular MOOCs. (2019) NPJ Sci Learn. 4:7. DOI: 10.1038/s41539-019-0046-0

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Garg, A.K., Li, P., Rashid, M.S., Callaway, E.M. Color and orientation are jointly coded and spatially organized in primate primary visual cortex. (2019) Ciencia. 364(6447):1275-1279. DOI: 10.1126/science.aaw5868

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Ernst, O.K., Bartol, T.M., Sejnowski, T.J., Mjolsness, E. Learning moment closure in reaction-diffusion systems with spatial dynamic Boltzmann distributions. (2019) Phys Rev E. 99(6-1):063315. DOI: 10.1103/PhysRevE.99.063315

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Herdy, J., Schafer, S., Kim, Y., Ansari, Z., Zangwill, D., Ku, M., Paquola, A., Lee, H., Mertens, J., Gage, F.H. Modulación química de vías de señalización enriquecidas transcripcionalmente para optimizar la conversión de fibroblastos en neuronas. (2019) Elife. 8. DOI: 10.7554/eLife.41356

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Das, A., Sexton, D., Lainscsek, C., Cash, S.S., Sejnowski, T.J. Characterizing Brain Connectivity from Human Electrocorticography Recordings with Unobserved Inputs during Epileptic Seizures. (2019) Computación neuronal.:1-56 DOI: 10.1162/neco_a_01205

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Epilepsy is a neurological disorder characterized by the sudden occurrence of unprovoked seizures. There is extensive evidence of significantly altered brain connectivity during seizure periods in the human brain. Research on analyzing human brain functional connectivity during epileptic seizures has been limited predominantly to the use of the correlation method. However, spurious connectivity can be measured between two brain regions without having direct connection or interaction between them. Correlations can be due to the apparent interactions of the two brain regions resulting from common input from a third region, which may or may not be observed. Hence, researchers have recently proposed a sparse-plus-latent-regularized precision matrix (SLRPM) when there are unobserved or latent regions interacting with the observed regions. The SLRPM method yields partial correlations of the conditional statistics of the observed regions given the latent regions, thus identifying observed regions that are conditionally independent of both the observed and latent regions. We evaluate the performance of the methods using a spring-mass artificial network and assuming that some nodes cannot be observed, thus constituting the latent variables in the example. Several cases have been considered, including both sparse and dense connections, short-range and long-range connections, and a varying number of latent variables. The SLRPM method is then applied to estimate brain connectivity during epileptic seizures from human ECoG recordings. Seventy-four clinical seizures from five patients, all having complex partial epilepsy, were analyzed using SLRPM, and brain connectivity was quantified using modularity index, clustering coefficient, and eigenvector centrality. Furthermore, using a measure of latent inputs estimated by the SLRPM method, it was possible to automatically detect 72 of the 74 seizures with four false positives and find six seizures that were not marked manually.

Wang, M., Zhang, L., Gage, F.H. Modelado de trastornos neuropsiquiátricos utilizando células madre pluripotentes inducidas humanas. (2019) Proteínas y Células. 11(1):45-59. DOI: 10.1007/s13238-019-0638-8

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Schafer, S.T., Gage, F.H. Las células nerviosas del cerebro invaden tumores de próstata. (2019) Naturaleza. 569(7758):637-638. DOI: 10.1038/d41586-019-01461-7

Gage, F.H. Neurogénesis adulta en mamíferos. (2019) Ciencia. 364(6443):827-828. DOI: 10.1126/science.aav6885

Siddharth, Patel, A.N., Jung, T.P., Sejnowski, T.J. A Wearable Multi-Modal Bio-Sensing System Towards Real-World Applications. (2019) IEEE Trans Biomed Eng. 66(4):1137-1147. DOI: 10.1109/TBME.2018.2868759

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Tapia, J.J., Saglam, A.S., Czech, J., Kuczewski, R., Bartol, T.M., Sejnowski, T.J., Faeder, J.R. MCell-R: A Particle-Resolution Network-Free Spatial Modeling Framework. (2019) Métodos en Biología Molecular. 1945:203-229. DOI: 10.1007/978-1-4939-9102-0_9

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Gatto, G., Smith, K.M., Ross, S.E., Goulding, M. Neuronal diversity in the somatosensory system: bridging the gap between cell type and function. (2019) Current Opinion in Neurobiology. 56:167-174. DOI: 10.1016/j.conb.2019.03.002

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Osseward, P.J., Pfaff, S.L. Cell type and circuit modules in the spinal cord. (2019) Current Opinion in Neurobiology. 56:175-184. DOI: 10.1016/j.conb.2019.03.003

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Berkowitz, J.A., Sharpee, T.O. Quantifying Information Conveyed by Large Neuronal Populations. (2019) Computación neuronal.:1-33 DOI: 10.1162/neco_a_01193

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McBride, E.G., Lee, S.J., Callaway, E.M. Local and Global Influences of Visual Spatial Selection and Locomotion in Mouse Primary Visual Cortex. (2019) Biología Actual. 29(10):1592-1605. DOI: 10.1016/j.cub.2019.03.065

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Nudell, V., Wei, H., Nievergelt, C., Maihofer, A.X., Shilling, P., Alda, M., Berrettini, W.H., Brennand, K.J., Calabrese, J.R., Coryell, W.H., Covault, J.M., Frye, M.A., Gage, F., Gershon, E., McInnis, M.G., Nurnberger, J.I., Oedegaard, K.J., Shekhtman, T., Zandi, P.P., Kelsoe, J.R., McCarthy, M.J. Aclimatación de Ritmos Circadianos a la Temperatura Revela Déficits de Amplitud en Fibroblastos de Pacientes con Trastorno Bipolar y Posibles Vínculos con Canales de Calcio. (2019) Neuropsiquiatría molecular. 5(2):115-124. DOI: 10.1159/000497354

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Wang, M., Zhang, L., Gage, F.H. Microglía, complemento y esquizofrenia. (2019) Nature Neuroscience. 22(3):333-334. DOI: 10.1038/s41593-019-0343-1

Farrelly, L.A., Thompson, R.E., Zhao, S., Lepack, A.E., Lyu, Y., Bhanu, N.V., Zhang, B., Loh, Y.E., Ramakrishnan, A., Vadodaria, K.C., Heard, K.J., Erikson, G., Nakadai, T., Bastle, R.M., Lukasak, B.J., Zebroski, H., Alenina, N., Bader, M., Berton, O., Roeder, R.G., Molina, H., Gage, F.H., Shen, L., Garcia, B.A., Li, H., Muir, T.W., Maze, I. La serotonilación de histonas es una modificación permisiva que mejora la unión de TFIID a H3K4me3. (2019) Naturaleza. DOI: 10.1038/s41586-019-1024-7

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Bohaciakova, D., Hruska-Plochan, M., Tsunemoto, R., Gifford, W.D., Driscoll, S.P., Glenn, T.D., Wu, S., Marsala, S., Navarro, M., Tadokoro, T., Juhas, S., Juhasova, J., Platoshyn, O., Piper, D., Sheckler, V., Ditsworth, D., Pfaff, S.L., Marsala, M. A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors. (2019) Stem Cell Research & Therapy. 10(1):83. DOI: 10.1186/s13287-019-1163-7

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Vadodaria, K.C., Ji, Y., Skime, M., Paquola, A.C., Nelson, T., Hall-Flavin, D., Heard, K.J., Fredlender, C., Deng, Y., Elkins, J., Dani, K., Le, A.T., Marchetto, M.C., Weinshilboum, R., Gage, F.H. Circuitería serotoninérgica alterada en neuronas derivadas de pacientes con trastorno depresivo mayor resistente a ISRS. (2019) Psiquiatría Molecular. DOI: 10.1038/s41380-019-0377-5

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Bonanomi, D., Valenza, F., Chivatakarn, O., Sternfeld, M.J., Driscoll, S.P., Aslanian, A., Lettieri, K., Gullo, M., Badaloni, A., Lewcock, J.W., Hunter, T., Pfaff, S.L. p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts. (2019) Neurona. DOI: 10.1016/j.neuron.2019.02.034

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Marchetto, M.C., Hrvoj-Mihic, B., Kerman, B.E., Yu, D.X., Vadodaria, K.C., Linker, S.B., Narvaiza, I., Santos, R., Denli, A.M., Mendes, A.P., Oefner, R., Cook, J., McHenry, L., Grasmick, J.M., Heard, K., Fredlender, C., Randolph-Moore, L., Kshirsagar, R., Xenitopoulos, R., Chou, G., Hah, N., Muotri, A.R., Padmanabhan, K., Semendeferi, K., Gage, F.H. Perfiles de maduración específicos de especies de células neurales humanas, de chimpancés y bonobos. (2019) Elife. 8. DOI: 10.7554/eLife.37527

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Aimon, S., Katsuki, T., Jia, T., Grosenick, L., Broxton, M., Deisseroth, K., Sejnowski, T.J., Greenspan, R.J. Fast near-whole-brain imaging in adult Drosophila during responses to stimuli and behavior. (2019) PLOS Biología. 17(2):e2006732. DOI: 10.1371/journal.pbio.2006732

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Lainscsek, C., Sampson, A.L., Kim, R., Thomas, M.L., Man, K., Lainscsek, X., , Swerdlow, N.R., Braff, D.L., Sejnowski, T.J., Light, G.A. Nonlinear dynamics underlying sensory processing dysfunction in schizophrenia. (2019) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 116(9):3847-3852. DOI: 10.1073/pnas.1810572116

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Bennett, C., Gale, S.D., Garrett, M.E., Newton, M.L., Callaway, E.M., Murphy, G.J., Olsen, S.R. Higher-Order Thalamic Circuits Channel Parallel Streams of Visual Information in Mice. (2019) Neurona. 102(2):477-492. DOI: 10.1016/j.neuron.2019.02.010

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Lucassen, P.J., Toni, N., Kempermann, G., Frisen, J., Gage, F.H., Swaab, D.F. ¿Límites a la neurogénesis humana, en serio? (2019) Psiquiatría Molecular. DOI: 10.1038/s41380-018-0337-5

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Schafer, S.T., Paquola, A.C.M., Stern, S., Gosselin, D., Ku, M., Pena, M., Kuret, T.J.M., Liyanage, M., Mansour, A.A., Jaeger, B.N., Marchetto, M.C., Glass, C.K., Mertens, J., Gage, F.H. La imprimación patológica causa heterocronía en redes genéticas del desarrollo en neuronas derivadas de sujetos autistas. (2019) Nature Neuroscience. DOI: 10.1038/s41593-018-0295-x

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Eftekharzadeh, B., Daigle, J.G., Kapinos, L.E., Coyne, A., Schiantarelli, J., Carlomagno, Y., Cook, C., Miller, S.J., Dujardin, S., Amaral, A.S., Grima, J.C., Bennett, R.E., Tepper, K., DeTure, M., Vanderburg, C.R., Corjuc, B.T., DeVos, S.L., Gonzalez, J.A., Chew, J., Vidensky, S., Gage, F.H., Mertens, J., Troncoso, J., Mandelkow, E., Salvatella, X., Lim, R.Y.H., Petrucelli, L., Wegmann, S., Rothstein, J.D., Hyman, B.T. La proteína tau altera el transporte nucleocitoplasmático en la enfermedad de Alzheimer. (2019) Neurona. 101(2):349. DOI: 10.1016/j.neuron.2018.12.031

Padmanabhan, K., Osakada, F., Tarabrina, A., Kizer, E., Callaway, E.M., Gage, F.H., Sejnowski, T.J. Entradas Centrifugas al Bulbo Olfatorio Principal Reveladas Mediante Mapeo de Circuitos de Cerebro Completo. (2019) Fronteras en Neuroanatomía. 12:115. DOI: 10.3389/fnana.2018.00115

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Yetman, M.J., Washburn, E., Hyun, J.H., Osakada, F., Hayano, Y., Zeng, H., Callaway, E.M., Kwon, H.B., Taniguchi, H. Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. (2019) Nature Neuroscience. 22:492-502. DOI: 10.1038/s41593-018-0322-y

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Vadodaria, K.C., Ji, Y., Skime, M., Paquola, A., Nelson, T., Hall-Flavin, D., Fredlender, C., Heard, K.J., Deng, Y., Le, A.T., Dave, S., Fung, L., Li, X., Marchetto, M.C., Weinshilboum, R., Gage, F.H. Hiperactividad inducida por serotonina en neuronas derivadas de pacientes con trastorno depresivo mayor resistente a ISRS. (2019) Psiquiatría Molecular. DOI: 10.1038/s41380-019-0363-y

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Sampson, A.L., Lainscsek, C., Gonzalez, C.E., Ulbert, I., Devinsky, O., Fabó, D., Halgren, E., Cash, S.S., Sejnowski, T.J. Delay Differential Analysis for Dynamical Sleep Spindle Detection. (2019) Journal of Neuroscience Methods. DOI: 10.1016/j.jneumeth.2019.01.009

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Huang, T., Lin, S.H., Malewicz, N.M., Zhang, Y., Zhang, Y., Goulding, M., LaMotte, R.H., Ma, Q. Identifying the pathways required for coping behaviours associated with sustained pain. (2018) Naturaleza. 565(7737):86-90. DOI: 10.1038/s41586-018-0793-8

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Kim, Y., Vadodaria, K.C., Lenkei, Z., Kato, T., Gage, F.H., Marchetto, M.C., Santos, R. MITOCONDRIAS, METABOLISMO Y MECANISMOS REDOX EN TRASTORNOS PSIQUIÁTRICOS. (2018) Antioxidantes y señalización redox. DOI: 10.1089/ars.2018.7606

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McCarthy, M.J., Wei, H., Nievergelt, C.M., Stautland, A., Maihofer, A.X., Welsh, D.K., Shilling, P., Alda, M., Alliey-Rodriguez, N., Anand, A., Andreasson, O.A., Balaraman, Y., Berrettini, W.H., Bertram, H., Brennand, K.J., Calabrese, J.R., Calkin, C.V., Claasen, A., Conroy, C., Coryell, W.H., Craig, D.W., D'Arcangelo, N., Demodena, A., Djurovic, S., Feeder, S., Fisher, C., Frazier, N., Frye, M.A., Gage, F.H., Gao, K., Garnham, J., Gershon, E.S., Glazer, K., Goes, F., Goto, T., Harrington, G., Jakobsen, P., Kamali, M., Karberg, E., Kelly, M., Leckband, S.G., Lohoff, F., McInnis, M.G., Mondimore, F., Morken, G., Nurnberger, J.I., Obral, S., Oedegaard, K.J., Ortiz, A., Ritchey, M., Ryan, K., Schinagle, M., Schoeyen, H., Schwebel, C., Shaw, M., Shekhtman, T., Slaney, C., Stapp, E., Szelinger, S., Tarwater, B., Zandi, P.P., Kelsoe, J.R. Cronomorfología y ritmos circadianos celulares predicen la respuesta clínica al tratamiento de mantenimiento con litio en pacientes con trastorno bipolar. (2018) Neuropsicofarmacología. . DOI: 10.1038/s41386-018-0273-8

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El trastorno bipolar (TB) es un trastorno grave del estado de ánimo asociado a anomalías en el ritmo circadiano. El riesgo de padecer TB está codificado genéticamente y se superpone con los sistemas que mantienen los ritmos circadianos. El litio es un tratamiento estabilizador del estado de ánimo eficaz para el TB, pero solo una minoría de pacientes responde plenamente a la monoterapia. Actualmente, planteamos la hipótesis de que los pacientes con TB que responden al litio (Li-R) mostrarían diferencias características en el cronotipo y los ritmos circadianos celulares en comparación con los que no responden al litio (Li-NR). Seleccionando pacientes de un ensayo clínico prospectivo y multicéntrico de monoterapia con litio, examinamos la preferencia por la mañana frente a la tarde (cronotipo) como una dimensión de la función del ritmo circadiano en 193 pacientes con TB Li-R y Li-NR. A partir de un subconjunto de 59 pacientes donantes, medimos los ritmos circadianos en fibroblastos de la piel de forma longitudinal durante 5 días utilizando un reportero bioluminiscente (Per2-luc). A continuación, estimamos los parámetros del ritmo circadiano (amplitud, período, fase) y los efectos farmacológicos del litio sobre los ritmos en las células de los donantes Li-R y Li-NR. En comparación con los Li-NR, los Li-R mostraron una diferencia en el cronotipo, con niveles más altos de «mañanismo». El cronotipo vespertino se asoció con un aumento de los síntomas del estado de ánimo en la línea de base, incluyendo depresión, manía e insomnio. Las células de los Li-R fueron más propensas a exhibir un período circadiano corto, una relación lineal entre el período y la fase, y efectos de acortamiento del período por el litio. La variación genética común en la vía de señalización IP podría explicar algunas de las diferencias individuales en los efectos del litio sobre los ritmos celulares. Concluimos que los ritmos circadianos pueden influir en la respuesta al litio en el tratamiento de mantenimiento del trastorno bipolar.

Suh, H., Zhou, Q.G., Fernandez-Carasa, I., Clemenson, G.D., Pons-Espinal, M., Ro, E.J., Marti, M., Raya, A., Gage, F.H., Consiglio, A. Etiquetado a Largo Plazo de Células Madre Neurales Hipocampales Mediante un Vector Lentiviral. (2018) Fronteras en Neurociencia Molecular. 11:415. DOI: 10.3389/fnmol.2018.00415

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Dhande, O.S., Stafford, B.K., Franke, K., El-Danaf, R., Percival, K.A., Phan, A.H., Li, P., Hansen, B.J., Nguyen, P.L., Berens, P., Taylor, W.R., Callaway, E., Euler, T., Huberman, A.D. Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits. (2018) Diario de Neurociencia. DOI: 10.1523/JNEUROSCI.1784-18.2018

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The ability to detect moving objects is an ethologically salient function. Direction selective neurons have been identified in the retina, thalamus, and cortex of many species, but their homology has remained opaque. For instance, it is unknown whether direction-selective retinal ganglion cells (DSGCs) exist in primates, and if so, whether they are the equivalent to mouse and rabbit DSGCs. Here, we used a molecular/circuit approach in both sexes to address these issues. In mice, we identify the transcription factor Satb2 (Special AT-rich sequence-binding protein 2) as a selective marker for three RGC types: On-Off DSGCs encoding motion in either the anterior or posterior direction, a newly identified type of Off-DSGC and an Off-sustained RGC type. In rabbits, we find that expression of Satb2 is conserved in On-Off DSGCs; however, has evolved to include On-Off DSGCs encoding upward and downward motion, in addition to anterior and posterior motion. Next, we show that macaque RGCs express Satb2 most likely in a single type. We used rabies-virus based circuit mapping tools to reveal the identity of macaque Satb2-RGCs and discovered their dendritic arbors are relatively large and monostratified. Together, these data indicate Satb2-expressing On-Off DSGCs are likely not present in the primate retina. Moreover, if DSGCs are present in the primate retina, it is unlikely that they express Satb2.The ability to detect object-motion is a fundamental feature of almost all visual systems. Here we identify a novel marker for retinal ganglion cells encoding directional motion that is evolutionarily conserved in mice and rabbits, but not in primates. We show that in macaque monkeys the retinal ganglion cells that express this marker comprise a single type and are morphologically distinct from mouse and rabbit direction-selective retinal ganglion cells. Our findings indicate that On-Off direction-selective retinal neurons may have evolutionarily diverged in primates and more generally provide novel insight into the identity and organization of primate parallel visual pathways.

Kuhn, H.G., Toda, T., Gage, F.H. Neurogénesis hipocampal adulta: una historia de maduración. (2018) Diario de Neurociencia. 10.1523/JNEUROSCI.2144-18.2018

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Reddy, G., Wong-Ng, J., Celani, A., Sejnowski, T.J., Vergassola, M. Glider soaring via reinforcement learning in the field. (2018) Naturaleza. DOI: 10.1038/s41586-018-0533-0

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Eftekharzadeh, B., Daigle, J.G., Kapinos, L.E., Coyne, A., Schiantarelli, J., Carlomagno, Y., Cook, C., Miller, S.J., Dujardin, S., Amaral, A.S., Grima, J.C., Bennett, R.E., Tepper, K., DeTure, M., Vanderburgh, C.R., Corjuc, B.T., DeVos, S.L., Gonzalez, J.A., Chew, J., Vidensky, S., Gage, F.H., Mertens, J., Troncoso, J., Mandelkow, E., Salvatella, X., Lim, R.Y.H., Petrucelli, L., Wegmann, S., Rothstein, J.D., Hyman, B.T. La proteína tau altera el transporte nucleocitoplasmático en la enfermedad de Alzheimer. (2018) Neurona. 99(5):925-940.e7. DOI: 10.1016/j.neuron.2018.07.039

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Choi, S.H., Bylykbashi, E., Chatila, Z.K., Lee, S.W., Pulli, B., Clemenson, G.D., Kim, E., Rompala, A., Oram, M.K., Asselin, C., Aronson, J., Zhang, C., Miller, S.J., Lesinski, A., Chen, J.W., Kim, D.Y., van Praag, H., Spiegelman, B.M., Gage, F.H., Tanzi, R.E. Neurogénesis adulta combinada y BDNF imitan los efectos del ejercicio en la cognición en un modelo de ratón con Alzheimer. (2018) Ciencia. 361(6406). DOI: 10.1126/science.aan8821

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Mertens, J., Reid, D., Lau, S., Kim, Y., Gage, F.H. Envejecimiento en una placa de cultivo: neuronas derivadas de iPSC e inducidas directamente para el estudio del envejecimiento cerebral y las enfermedades neurodegenerativas relacionadas con la edad. (2018) Revisión Anual de Genética. 52:271-293. DOI: 10.1146/annurev-genet-120417-031534

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Sejnowski, T. The prophet who foretold our future. (2018) Brain : A Journal of Neurology. 141(9):2820-2822. DOI: 10.1093/brain/awy212

Jaeger, B.N., Linker, S.B., Parylak, S.L., Barron, J.J., Gallina, I.S., Saavedra, C.D., Fitzpatrick, C., Lim, C.K., Schafer, S.T., Lacar, B., Jessberger, S., Gage, F.H. Una novedosa firma transcripcional evocada por el ambiente predice la reactividad en neuronas granulares del hipocampo en células individuales. (2018) Nature Communications. 9(1):3084. DOI: 10.1038/s41467-018-05418-8

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Zhang, Y., Liu, S., Zhang, Y.Q., Goulding, M., Wang, Y.Q., Ma, Q. Timing Mechanisms Underlying Gate Control by Feedforward Inhibition. (2018) Neurona. 99(5):941-955.e4. DOI: 10.1016/j.neuron.2018.07.026

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van den Hurk, M., Erwin, J.A., Yeo, G.W., Gage, F.H., Bardy, C. Protocolo Patch-Seq para analizar la electrofisiología, morfología y transcriptoma de neuronas únicas derivadas de células madre pluripotentes humanas. (2018) Fronteras en Neurociencia Molecular. 11:261. DOI: 10.3389/fnmol.2018.00261

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Zhou, Y., Smith, B.H., Sharpee, T.O. Hyperbolic geometry of the olfactory space. (2018) Avances en Ciencia. 4(8):eaaq1458. DOI: 10.1126/sciadv.aaq1458

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Sommer, A., Maxreiter, F., Krach, F., Fadler, T., Grosch, J., Maroni, M., Graef, D., Eberhardt, E., Riemenschneider, M.J., Yeo, G.W., Kohl, Z., Xiang, W., Gage, F.H., Winkler, J., Prots, I., Winner, B. Linfocitos Th17 Inducen Muerte Neuronal en un Modelo Basado en iPSC Humanas de la Enfermedad de Parkinson. (2018) Células Madre. 23(1):123-131.e6. DOI: 10.1016/j.stem.2018.06.015

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Linker, S.B., Hsu, J.Y., Pfaff, A., Amatya, D., Ko, S.M., Voter, S., Wong, Q., Gage, F.H. BrainImageR: Análisis espaciotemporal de conjuntos de genes haciendo referencia al cerebro humano. (2018) Bioinformática. DOI: 10.1093/bioinformatics/bty618

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Ernst, O.K., Bartol, T., Sejnowski, T., Mjolsness, E. Learning dynamic Boltzmann distributions as reduced models of spatial chemical kinetics. (2018) J Chem Phys. 149(3):034107. DOI: 10.1063/1.5026403

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Haas, K.T., Compans, B., Letellier, M., Bartol, T.M., Grillo-Bosch, D., Sejnowski, T.J., Sainlos, M., Choquet, D., Thoumine, O., Hosy, E. Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency. (2018) Elife. 7. DOI: 10.7554/eLife.31755

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Siddharth, Jung, T.P., Sejnowski, T.J. Multi-modal Approach for Affective Computing. (2018) Conf Proc IEEE Eng Med Biol Soc. 2018:291-294. DOI: 10.1109/EMBC.2018.8512320

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Kronenberg, Z.N., Fiddes, I.T., Gordon, D., Murali, S., Cantsilieris, S., Meyerson, O.S., Underwood, J.G., Nelson, B.J., Chaisson, M.J.P., Dougherty, M.L., Munson, K.M., Hastie, A.R., Diekhans, M., Hormozdiari, F., Lorusso, N., Hoekzema, K., Qiu, R., Clark, K., Raja, A., Welch, A.E., Sorensen, M., Baker, C., Fulton, R.S., Armstrong, J., Graves-Lindsay, T.A., Denli, A.M., Hoppe, E.R., Hsieh, P., Hill, C.M., Pang, A.W.C., Lee, J., Lam, E.T., Dutcher, S.K., Gage, F.H., Warren, W.C., Shendure, J., Haussler, D., Schneider, V.A., Cao, H., Ventura, M., Wilson, R.K., Paten, B., Pollen, A., Eichler, E.E. Análisis comparativo de genomas de grandes simios en alta resolución. (2018) Ciencia. 360(6393). DOI: 10.1126/science.aar6343

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Krishnan, G.P., Rosen, B.Q., Chen, J.Y., Muller, L., Sejnowski, T.J., Cash, S.S., Halgren, E., Bazhenov, M. Thalamocortical and intracortical laminar connectivity determines sleep spindle properties. (2018) PLOS Biología Computacional. 14(6):e1006171. DOI: 10.1371/journal.pcbi.1006171

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Ueno, M., Nakamura, Y., Li, J., Gu, Z., Niehaus, J., Maezawa, M., Crone, S.A., Goulding, M., Baccei, M.L., Yoshida, Y. Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons. (2018) Cell Reports. 23(5):1286-1300.e7. DOI: 10.1016/j.celrep.2018.03.137

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Sarkar, A., Mei, A., Paquola, A.C.M., Stern, S., Bardy, C., Klug, J.R., Kim, S., Neshat, N., Kim, H.J., Ku, M., Shokhirev, M.N., Adamowicz, D.H., Marchetto, M.C., Jappelli, R., Erwin, J.A., Padmanabhan, K., Shtrahman, M., Jin, X., Gage, F.H. Generación Eficiente de Neuronas CA3 a Partir de Células Madre Pluripotentes Humanas Permite el Modelado de la Conectividad Hipocampal In Vitro. (2018) Células Madre. 22(5):684-697.e9. DOI: 10.1016/j.stem.2018.04.009

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Strnadel, J., Carromeu, C., Bardy, C., Navarro, M., Platoshyn, O., Glud, A.N., Marsala, S., Kafka, J., Miyanohara, A., Kato, T., Tadokoro, T., Hefferan, M.P., Kamizato, K., Yoshizumi, T., Juhas, S., Juhasova, J., Ho, C.S., Kheradmand, T., Chen, P., Bohaciakova, D., Hruska-Plochan, M., Todd, A.J., Driscoll, S.P., Glenn, T.D., Pfaff, S.L., Klima, J., Ciacci, J., Curtis, E., Gage, F.H., Bui, J., Yamada, K., Muotri, A.R., Marsala, M. Supervivencia de precursores neurales derivados de iPSC singénicos y alogénicos después de injertos espinales en minicerdos. (2018) Medicina Traslacional en Ciencia. 10(440). DOI: 10.1126/scitranslmed.aam6651

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Stern, S., Linker, S., Vadodaria, K.C., Marchetto, M.C., Gage, F.H. Prediction of response to drug therapy in psychiatric disorders. (2018) Open Biology. 8(5). DOI: 10.1098/rsob.180031

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Personalized medicine has become increasingly relevant to many medical fields, promising more efficient drug therapies and earlier intervention. The development of personalized medicine is coupled with the identification of biomarkers and classification algorithms that help predict the responses of different patients to different drugs. In the last 10 years, the Food and Drug Administration (FDA) has approved several genetically pre-screened drugs labelled as pharmacogenomics in the fields of oncology, pulmonary medicine, gastroenterology, haematology, neurology, rheumatology and even psychiatry. Clinicians have long cautioned that what may appear to be similar patient-reported symptoms may actually arise from different biological causes. With growing populations being diagnosed with different psychiatric conditions, it is critical for scientists and clinicians to develop precision medication tailored to individual conditions. Genome-wide association studies have highlighted the complicated nature of psychiatric disorders such as schizophrenia, bipolar disorder, major depression and autism spectrum disorder. Following these studies, association studies are needed to look for genomic markers of responsiveness to available drugs of individual patients within the population of a specific disorder. In addition to GWAS, the advent of new technologies such as brain imaging, cell reprogramming, sequencing and gene editing has given us the opportunity to look for more biomarkers that characterize a therapeutic response to a drug and to use all these biomarkers for determining treatment options. In this review, we discuss studies that were performed to find biomarkers of responsiveness to different available drugs for four brain disorders: bipolar disorder, schizophrenia, major depression and autism spectrum disorder. We provide recommendations for using an integrated method that will use available techniques for a better prediction of the most suitable drug.

Kim, Y., Zheng, X., Ansari, Z., Bunnell, M.C., Herdy, J.R., Traxler, L., Lee, H., Paquola, A.C.M., Blithikioti, C., Ku, M., Schlachetzki, J.C.M., Winkler, J., Edenhofer, F., Glass, C.K., Paucar, A.A., Jaeger, B.N., Pham, S., Boyer, L., Campbell, B.C., Hunter, T., Mertens, J., Gage, F.H. Mitochondrial Aging Defects Emerge in Directly Reprogrammed Human Neurons due to Their Metabolic Profile. (2018) Cell Reports. 23(9):2550-2558. DOI: 10.1016/j.celrep.2018.04.105

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Patriarchi, T., Cho, J.R., Merten, K., Howe, M.W., Marley, A., Xiong, W.H., Folk, R.W., Broussard, G.J., Liang, R., Jang, M.J., Zhong, H., Dombeck, D., von Zastrow, M., Nimmerjahn, A., Gradinaru, V., Williams, J.T., Tian, L. Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors. (2018) Ciencia. DOI: 10.1126/science.aat4422

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Mansour, A.A., Gonçalves, J.T., Bloyd, C.W., Li, H., Fernandes, S., Quang, D., Johnston, S., Parylak, S.L., Jin, X., Gage, F.H. An in vivo model of functional and vascularized human brain organoids. (2018) Nature Biotechnology. DOI: 10.1038/nbt.4127

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Luo, L., Callaway, E.M., Svoboda, K. Genetic Dissection of Neural Circuits: A Decade of Progress. (2018) Neurona. 98(2):256-281. DOI: 10.1016/j.neuron.2018.03.040

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Toda, T., Parylak, S.L., Linker, S.B., Gage, F.H. The role of adult hippocampal neurogenesis in brain health and disease. (2018) Psiquiatría Molecular. DOI: 10.1038/s41380-018-0036-2

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Kempermann, G., Gage, F.H., Aigner, L., Song, H., Curtis, M.A., Thuret, S., Kuhn, H.G., Jessberger, S., Frankland, P.W., Cameron, H.A., Gould, E., Hen, R., Abrous, D.N., Toni, N., Schinder, A.F., Zhao, X., Lucassen, P.J., Frisén, J. Human Adult Neurogenesis: Evidence and Remaining Questions. (2018) Células Madre. DOI: 10.1016/j.stem.2018.04.004

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Ruiz-Martínez, , Bartol, T.M., Sejnowski, T.J., Tartakovsky, D.M. Efficient models of polymerization applied to FtsZ ring assembly in . (2018) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1719391115

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Moldakarimov, S., Bazhenov, M., Feldman, D.E., Sejnowski, T.J. Structured networks support sparse traveling waves in rodent somatosensory cortex. (2018) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1710202115

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Wang, X., Pinto-Duarte, A., Behrens, M.M., Zhou, X., Sejnowski, T.J. Ketamine independently modulated power and phase-coupling of theta oscillations in Sp4 hypomorphic mice. (2018) PLOS One. 13(3):e0193446. DOI: 10.1371/journal.pone.0193446

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Reduced expression of Sp4, the murine homolog of human SP4, a risk gene of multiple psychiatric disorders, led to N-methyl-D-aspartate (NMDA) hypofunction in mice, producing behavioral phenotypes reminiscent of schizophrenia, including hypersensitivity to ketamine. As accumulating evidence on molecular mechanisms and behavioral phenotypes established Sp4 hypomorphism as a promising animal model, systems-level neural circuit mechanisms of Sp4 hypomorphism, especially network dynamics underlying cognitive functions, remain poorly understood. We attempted to close this gap in knowledge in the present study by recording multi-channel epidural electroencephalogram (EEG) from awake behaving wildtype and Sp4 hypomorphic mice. We characterized cortical theta-band power and phase-coupling phenotypes, a known neural circuit substrate underlying cognitive functions, and further studied the effects of a subanesthetic dosage of ketamine on theta abnormalities unique to Sp4 hypomorphism. Sp4 hypomorphic mice had markedly elevated theta power localized frontally and parietally, a more pronounced theta phase progression along the neuraxis, and a stronger frontal-parietal theta coupling. Acute subanesthetic ketamine did not affect theta power in wildtype animals but significantly reduced it in Sp4 hypomorphic mice, nearly completely neutralizing their excessive frontal/parietal theta power. Ketamine did not significantly alter cortical theta phase progression in either wildtype or Sp4 hypomorphic animals, but significantly strengthened cortical theta phase-coupling in wildtype, but not in Sp4 hypomorphic animals. Our results suggested that the resting-state phenotypes of cortical theta oscillations unique to Sp4 hypomorphic mice closely mimicked a schizophrenic endophenotype. Further, ketamine independently modulated Sp4 hypomorphic anomalies in theta power and phase-coupling, suggesting separate underlying neural circuit mechanisms.

Mo, Z., Zhao, X., Liu, H., Hu, Q., Chen, X.Q., Pham, J., Wei, N., Liu, Z., Zhou, J., Burgess, R.W., Pfaff, S.L., Caskey, C.T., Wu, C., Bai, G., Yang, X.L. Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy. (2018) Nature Communications. 9(1):1007. DOI: 10.1038/s41467-018-03461-z

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Gatto, G., Goulding, M. Locomotion Control: Brainstem Circuits Satisfy the Need for Speed. (2018) Biología Actual. 28(6):R256-R259. DOI: 10.1016/j.cub.2018.01.068

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Muller, L., Chavane, F., Reynolds, J., Sejnowski, T.J. Cortical travelling waves: mechanisms and computational principles. (2018) Nature Reviews Neuroscience. DOI: 10.1038/nrn.2018.20

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Bedrosian, T.A., Quayle, C., Novaresi, N., Gage, F.H. Early life experience drives structural variation of neural genomes in mice. (2018) Ciencia. 359(6382):1395-1399. DOI: 10.1126/science.aah3378

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Zhang, Y., Kastner, D.B., Baccus, S.A., Sharpee, T.O. Optimal Information Transmission by Overlapping Retinal Cell Mosaics. (2018) Proc Conf Inf Sci Syst. 2018. DOI: 10.1109/ciss.2018.8362310

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Zheng, Y., Shen, W., Zhang, J., Yang, B., Liu, Y.N., Qi, H., Yu, X., Lu, S.Y., Chen, Y., Xu, Y.Z., Li, Y., Gage, F.H., Mi, S., Yao, J. CRISPR interference-based specific and efficient gene inactivation in the brain. (2018) Nature Neuroscience. DOI: 10.1038/s41593-018-0077-5

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Kaya, C., Cheng, M.H., Block, E.R., Bartol, T.M., Sejnowski, T.J., Sorkin, A., Faeder, J.R., Bahar, I. Heterogeneities in Axonal Structure and Transporter Distribution Lower Dopamine Reuptake Efficiency. (2018) eNeuro. 5(1). DOI: 10.1523/ENEURO.0298-17.2017

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Bromer, C., Bartol, T.M., Bowden, J.B., Hubbard, D.D., Hanka, D.C., Gonzalez, P.V., Kuwajima, M., Mendenhall, J.M., Parker, P.H., Abraham, W.C., Sejnowski, T.J., Harris, K.M. Long-term potentiation expands information content of hippocampal dentate gyrus synapses. (2018) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1716189115

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Vadodaria, K.C., Amatya, D.N., Marchetto, M.C., Gage, F.H. Modeling psychiatric disorders using patient stem cell-derived neurons: a way forward. (2018) Genome Medicine. 10(1):1. DOI: 10.1186/s13073-017-0512-3

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Wang, M., He, Y., Sejnowski, T.J., Yu, X. Brain-state dependent astrocytic Ca2+ signals are coupled to both positive and negative BOLD-fMRI signals. (2018) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1711692115

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Hayashi, M., Hinckley, C.A., Driscoll, S.P., Moore, N.J., Levine, A.J., Hilde, K.L., Sharma, K., Pfaff, S.L. Graded Arrays of Spinal and Supraspinal V2a Interneuron Subtypes Underlie Forelimb and Hindlimb Motor Control. (2018) Neurona. DOI: 10.1016/j.neuron.2018.01.023

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Sharpee, T.O. (2018) Oxford Research Encyclopedias, Neuroscience.:1-23 DOI: doi.org/10.1093/acrefore/9780190264086.013.110

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Sensory systems exist to provide an organism with information about the state of the environment that can be used to guide future actions and decisions. Remarkably, two conceptually simple yet general theorems from information theory can be used to evaluate the performance of any sensory system. One theorem states that there is a minimal amount of energy that an organism has to spend in order to capture a given amount of information about the environment. The second theorem states that the maximum rate with which the organism can acquire resources from the environment, relative to its competitors, is limited by the information this organism collects about the environment, also relative to its competitors. These two theorems provide a scaffold for formulating and testing general principles of sensory coding but leave unanswered many important practical questions of implementation in neural circuits. These implementation questions have guided thinking in entire subfields of sensory neuroscience, and include: What features in the sensory environment should be measured? Given that we make decisions on a variety of time scales, how should one solve trade-offs between making simpler measurements to guide minimal decisions vs. more elaborate sensory systems that have to overcome multiple delays between sensation and action. Once we agree on the types of features that are important to represent, how should they be represented? How should resources be allocated between different stages of processing, and where is the impact of noise most damaging? Finally, one should consider trade-offs between implementing a fixed strategy vs. an adaptive scheme that readjusts resources based on current needs. Where adaptation is considered, under what conditions does it become optimal to switch strategies? Research over the past 60 years has provided answers to almost all of these questions but primarily in early sensory systems. Joining these answers into a comprehensive framework is a challenge that will help us understand who we are and how we can make better use of limited natural resources.

Koch, S.C., Del Barrio, M.G., Dalet, A., Gatto, G., Günther, T., Zhang, J., Seidler, B., Saur, D., Schüle, R., Goulding, M. RORβ Spinal Interneurons Gate Sensory Transmission during Locomotion to Secure a Fluid Walking Gait. (2017) Neurona. DOI: 10.1016/j.neuron.2017.11.011

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Jaeger, B.N., Parylak, S.L., Gage, F.H. Mechanisms of dietary flavonoid action in neuronal function and neuroinflammation. (2017) Molecular Aspects of Medicine. DOI: 10.1016/j.mam.2017.11.003

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Callaway, E.M., Garg, A.K. Brain technology: Neurons recorded en masse. (2017) Naturaleza. 551(7679):172-173. DOI: 10.1038/551172a

Toda, T., Gage, F.H. Review: adult neurogenesis contributes to hippocampal plasticity. (2017) Cell and Tissue Research. DOI: 10.1007/s00441-017-2735-4

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O'Donnell, C., Gonçalves, J.T., Portera-Cailliau, C., Sejnowski, T.J. Beyond excitation/inhibition imbalance in multidimensional models of neural circuit changes in brain disorders. (2017) Elife. 6. DOI: 10.7554/eLife.26724

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Snijders, C., de Nijs, L., Baker, D.G., Hauger, R.L., van den Hove, D., Kenis, G., Nievergelt, C.M., Boks, M.P., Vermetten, E., Gage, F.H., Rutten, B.P.F. MicroRNAs in Post-traumatic Stress Disorder. (2017) Current Topics in Behavioral Neurosciences. DOI: 10.1007/7854_2017_32

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Atencio, C.A., Sharpee, T.O. Multidimensional receptive field processing by cat primary auditory cortical neurons. (2017) Neuroscience. 359:130-141. DOI: 10.1016/j.neuroscience.2017.07.003

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Topol, A., Zhu, S., Hartley, B.J., English, J., Hauberg, M.E., Tran, N., Rittenhouse, C.A., Simone, A., Ruderfer, D.M., Johnson, J., Readhead, B., Hadas, Y., Gochman, P.A., Wang, Y.C., Shah, H., Cagney, G., Rapoport, J., Gage, F.H., Dudley, J.T., Sklar, P., Mattheisen, M., Cotter, D., Fang, G., Brennand, K.J. Dysregulation of miRNA-9 in a Subset of Schizophrenia Patient-Derived Neural Progenitor Cells. (2017) Cell Reports. 20(10):2525. DOI: 10.1016/j.celrep.2017.08.073

Wu, J., Capelli, P., Bouvier, J., Goulding, M., Arber, S., Fortin, G. A V0 core neuronal circuit for inspiration. (2017) Nature Communications. 8(1):544. DOI: 10.1038/s41467-017-00589-2

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Toda, T., Hsu, J.Y., Linker, S.B., Hu, L., Schafer, S.T., Mertens, J., Jacinto, F.V., Hetzer, M.W., Gage, F.H. Nup153 Interacts with Sox2 to Enable Bimodal Gene Regulation and Maintenance of Neural Progenitor Cells. (2017) Células Madre. 21:618–634. DOI: 10.1016/j.stem.2017.08.012

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Shahsavani, M., Pronk, R.J., Falk, R., Lam, M., Moslem, M., Linker, S.B., Salma, J., Day, K., Schuster, J., Anderlid, B.M., Dahl, N., Gage, F.H., Falk, A. An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis. (2017) Psiquiatría Molecular. DOI: 10.1038/mp.2017.175

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Koch, S.C., Acton, D., Goulding, M. Spinal Circuits for Touch, Pain, and Itch. (2017) Annual Review of Physiology. DOI: 10.1146/annurev-physiol-022516-034303

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Sharpee, T.O. On texture, form, and fixational eye movements. (2017) Current Opinion in Neurobiology. 46:228-233. DOI: 10.1016/j.conb.2017.09.002

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Li, Y., Wang, F., Chen, Y., Cichocki, A., Sejnowski, T. The Effects of Audiovisual Inputs on Solving the Cocktail Party Problem in the Human Brain: An fMRI Study. (2017) Cerebral Cortex .:1-15 DOI: 10.1093/cercor/bhx235

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Holter, K.E., Kehlet, B., Devor, A., Sejnowski, T.J., Dale, A.M., Omholt, S.W., Ottersen, O.P., Nagelhus, E.A., Mardal, K.A., Pettersen, K.H. Interstitial solute transport in 3D reconstructed neuropil occurs by diffusion rather than bulk flow. (2017) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. DOI: 10.1073/pnas.1706942114

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Vadodaria, K.C., Stern, S., Marchetto, M.C., Gage, F.H. Serotonin in psychiatry: in vitro disease modeling using patient-derived neurons. (2017) Cell and Tissue Research. DOI: 10.1007/s00441-017-2670-4

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Linker, S.B., Marchetto, M.C., Narvaiza, I., Denli, A.M., Gage, F.H. Examining non-LTR retrotransposons in the context of the evolving primate brain. (2017) BMC Biology. 15(1):68. DOI: 10.1186/s12915-017-0409-z

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Luo, C., Keown, C.L., Kurihara, L., Zhou, J., He, Y., Li, J., Castanon, R., Lucero, J., Nery, J.R., Sandoval, J.P., Bui, B., Sejnowski, T.J., Harkins, T.T., Mukamel, E.A.*, Behrens, M.M.*, Ecker, J.R.* Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex. (2017) Ciencia. 357(6351):600-604. DOI: 10.1126/science.aan3351

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Lainscsek, C., Weyhenmeyer, J., Cash, S.S., Sejnowski, T.J. Delay Differential Analysis of Seizures in Multichannel Electrocorticography Data. (2017) Computación neuronal.:1-38 DOI: 10.1162/neco_a_01009

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Lin, T.W., Das, A., Krishnan, G.P., Bazhenov, M., Sejnowski, T.J. Differential Covariance: A New Class of Methods to Estimate Sparse Connectivity from Neural Recordings. (2017) Computación neuronal.:1-52 DOI: 10.1162/neco_a_01008

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Kaardal, J.T., Theunissen, F.E., Sharpee, T.O. A Low-Rank Method for Characterizing High-Level Neural Computations. (2017) Frontiers in Computational Neuroscience. 11:68. DOI: 10.3389/fncom.2017.00068

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Newkirk, S.J., Lee, S., Grandi, F.C., Gaysinskaya, V., Rosser, J.M., Vanden Berg, N., Hogarth, C.A., Marchetto, M.C.N., Muotri, A.R., Griswold, M.D., Ye, P., Bortvin, A., Gage, F.H., Boeke, J.D., An, W. Intact piRNA pathway prevents L1 mobilization in male meiosis. (2017) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 114(28):E5635-E5644. DOI: 10.1073/pnas.1701069114

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Lainscsek, C., Muller, L.E., Sampson, A.L., Sejnowski, T.J. Analytical Derivation of Nonlinear Spectral Effects and 1/f Scaling Artifact in Signal Processing of Real-World Data. (2017) Computación neuronal. 29(7):2004-2020. DOI: 10.1162/NECO_a_00979

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Veit, J., Hakim, R., Jadi, M.P., Sejnowski, T.J., Adesnik, H. Cortical gamma band synchronization through somatostatin interneurons. (2017) Nature Neuroscience. 20(7):951-959. DOI: 10.1038/nn.4562

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Hurtado-López, J., Ramirez-Moreno, D.F., Sejnowski, T.J. Decision-making neural circuits mediating social behaviors : An attractor network model. (2017) Journal of Computational Neuroscience. DOI: 10.1007/s10827-017-0654-8

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Sharpee, T.O. Optimizing Neural Information Capacity through Discretization. (2017) Neurona. 94(5):954-960. DOI: 10.1016/j.neuron.2017.04.044

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Rowekamp, R.J., Sharpee, T.O. Cross-orientation suppression in visual area V2. (2017) Nature Communications. 8:15739. DOI: 10.1038/ncomms15739

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Santos, R., Vadodaria, K.C., Jaeger, B.N., Mei, A., Lefcochilos-Fogelquist, S., Mendes, A.P.D., Erikson, G., Shokhirev, M., Randolph-Moore, L., Fredlender, C., Dave, S., Oefner, R., Fitzpatrick, C., Pena, M., Barron, J.J., Ku, M., Denli, A.M., Kerman, B.E., Charnay, P., Kelsoe, J.R., Marchetto, M.C., Gage, F.H. Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells. (2017) Stem Cell Reports. 8(6):1757-1769. DOI: 10.1016/j.stemcr.2017.05.011

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Gosselin, D., Skola, D., Coufal, N.G., Holtman, I.R., Schlachetzki, J.C.M., Sajti, E., Jaeger, B.N., O'Connor, C., Fitzpatrick, C., Pasillas, M.P., Pena, M., Adair, A., Gonda, D.D., Levy, M.L., Ransohoff, R.M., Gage, F.H., Glass, C.K. An environment-dependent transcriptional network specifies human microglia identity. (2017) Ciencia. 356(6344). DOI: 10.1126/science.aal3222

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Cheng, L., Duan, B., Huang, T., Zhang, Y., Chen, Y., Britz, O., Garcia-Campmany, L., Ren, X., Vong, L., Lowell, B.B., Goulding, M., Wang, Y., Ma, Q. Identification of spinal circuits involved in touch-evoked dynamic mechanical pain. (2017) Nature Neuroscience. 20(6):804-814. DOI: 10.1038/nn.4549

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Marchetto, M.C., Belinson, H., Tian, Y., Freitas, B.C., Fu, C., Vadodaria, K., Beltrao-Braga, P., Trujillo, C.A., Mendes, A.P.D., Padmanabhan, K., Nunez, Y., Ou, J., Ghosh, H., Wright, R., Brennand, K., Pierce, K., Eichenfield, L., Pramparo, T., Eyler, L., Barnes, C.C., Courchesne, E., Geschwind, D.H., Gage, F.H., Wynshaw-Boris, A., Muotri, A.R. Altered proliferation and networks in neural cells derived from idiopathic autistic individuals. (2017) Psiquiatría Molecular. 22(6):820-835. DOI: 10.1038/mp.2016.95

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Ishii, S., Torii, M., Son, A.I., Rajendraprasad, M., Morozov, Y.M., Kawasawa, Y.I., Salzberg, A.C., Fujimoto, M., Brennand, K., Nakai, A., Mezger, V., Gage, F.H., Rakic, P., Hashimoto-Torii, K. Variations in brain defects result from cellular mosaicism in the activation of heat shock signalling. (2017) Nature Communications. 8:15157. DOI: 10.1038/ncomms15157

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McConnell, M.J., Moran, J.V., Abyzov, A., Akbarian, S., Bae, T., Cortes-Ciriano, I., Erwin, J.A., Fasching, L., Flasch, D.A., Freed, D., Ganz, J., Jaffe, A.E., Kwan, K.Y., Kwon, M., Lodato, M.A., Mills, R.E., Paquola, A.C.M., Rodin, R.E., Rosenbluh, C., Sestan, N., Sherman, M.A., Shin, J.H., Song, S., Straub, R.E., Thorpe, J., Weinberger, D.R., Urban, A.E., Zhou, B., Gage, F.H., Lehner, T., Senthil, G., Walsh, C.A., Chess, A., Courchesne, E., Gleeson, J.G., Kidd, J.M., Park, P.J., Pevsner, J., Vaccarino, F.M. Intersection of diverse neuronal genomes and neuropsychiatric disease: The Brain Somatic Mosaicism Network. (2017) Ciencia. 356(6336). DOI: 10.1126/science.aal1641

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Peterson, D.A., Sejnowski, T.J. A Dynamic Circuit Hypothesis for the Pathogenesis of Blepharospasm. (2017) Frontiers in Computational Neuroscience. 11:11. DOI: 10.3389/fncom.2017.00011

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Blepharospasm (sometimes called "benign essential blepharospasm," BEB) is one of the most common focal dystonias. It involves involuntary eyelid spasms, eye closure, and increased blinking. Despite the success of botulinum toxin injections and, in some cases, pharmacologic or surgical interventions, BEB treatments are not completely efficacious and only symptomatic. We could develop principled strategies for preventing and reversing the disease if we knew the pathogenesis of primary BEB. The objective of this study was to develop a conceptual framework and dynamic circuit hypothesis for the pathogenesis of BEB. The framework extends our overarching theory for the multifactorial pathogenesis of focal dystonias (Peterson et al., 2010) to incorporate a two-hit rodent model specifically of BEB (Schicatano et al., 1997). We incorporate in the framework three features critical to cranial motor control: (1) the joint influence of motor cortical regions and direct descending projections from one of the basal ganglia output nuclei, the substantia nigra pars reticulata, on brainstem motor nuclei, (2) nested loops composed of the trigeminal blink reflex arc and the long sensorimotor loop from trigeminal nucleus through thalamus to somatosensory cortex back through basal ganglia to the same brainstem nuclei modulating the reflex arc, and (3) abnormalities in the basal ganglia dopamine system that provide a sensorimotor learning substrate which, when combined with patterns of increased blinking, leads to abnormal sensorimotor mappings manifest as BEB. The framework explains experimental data on the trigeminal reflex blink excitability (TRBE) from Schicatano et al. and makes predictions that can be tested in new experimental animal models based on emerging genetics in dystonia, including the recently characterized striatal-specific D1R dopamine transduction alterations caused by the GNAL mutation. More broadly, the model will provide a guide for future efforts to mechanistically link multiple factors in the pathogenesis of BEB and facilitate simulations of how exogenous manipulations of the pathogenic factors could ultimately be used to prevent and reverse the disorder.

Das, A., Sampson, A.L., Lainscsek, C., Muller, L., Lin, W., Doyle, J.C., Cash, S.S., Halgren, E., Sejnowski, T.J. Interpretation of the Precision Matrix and Its Application in Estimating Sparse Brain Connectivity during Sleep Spindles from Human Electrocorticography Recordings. (2017) Computación neuronal. 29(3):603-642. DOI: 10.1162/NECO_a_00936

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Kim, Y., Santos, R., Gage, F.H., Marchetto, M.C. Molecular Mechanisms of Bipolar Disorder: Progress Made and Future Challenges. (2017) Frontiers in Cellular Neuroscience. 11:30. DOI: 10.3389/fncel.2017.00030

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Ramsay, L., Marchetto, M.C., Caron, M., Chen, S.H., Busche, S., Kwan, T., Pastinen, T., Gage, F.H., Bourque, G. Conserved expression of transposon-derived non-coding transcripts in primate stem cells. (2017) BMC Genomics. 18(1):214. DOI: 10.1186/s12864-017-3568-y

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Stern, S., Santos, R., Marchetto, M.C., Mendes, A.P., Rouleau, G.A., Biesmans, S., Wang, Q.W., Yao, J., Charnay, P., Bang, A.G., Alda, M., Gage, F.H. Neurons derived from patients with bipolar disorder divide into intrinsically different sub-populations of neurons, predicting the patients' responsiveness to lithium. (2017) Psiquiatría Molecular. DOI: 10.1038/mp.2016.260

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Bipolar disorder (BD) is a progressive psychiatric disorder with more than 3% prevalence worldwide. Affected individuals experience recurrent episodes of depression and mania, disrupting normal life and increasing the risk of suicide greatly. The complexity and genetic heterogeneity of psychiatric disorders have challenged the development of animal and cellular models. We recently reported that hippocampal dentate gyrus (DG) neurons differentiated from induced pluripotent stem cell (iPSC)-derived fibroblasts of BD patients are electrophysiologically hyperexcitable. Here we used iPSCs derived from Epstein-Barr virus-immortalized B-lymphocytes to verify that the hyperexcitability of DG-like neurons is reproduced in this different cohort of patients and cells. Lymphocytes are readily available for research with a large number of banked lines with associated patient clinical description. We used whole-cell patch-clamp recordings of over 460 neurons to characterize neurons derived from control individuals and BD patients. Extensive functional analysis showed that intrinsic cell parameters are very different between the two groups of BD neurons, those derived from lithium (Li)-responsive (LR) patients and those derived from Li-non-responsive (NR) patients, which led us to partition our BD neurons into two sub-populations of cells and suggested two different subdisorders. Training a Naïve Bayes classifier with the electrophysiological features of patients whose responses to Li are known allows for accurate classification with more than 92% success rate for a new patient whose response to Li is unknown. Despite their very different functional profiles, both populations of neurons share a large, fast after-hyperpolarization (AHP). We therefore suggest that the large, fast AHP is a key feature of BD and a main contributor to the fast, sustained spiking abilities of BD neurons. Confirming our previous report with fibroblast-derived DG neurons, chronic Li treatment reduced the hyperexcitability in the lymphoblast-derived LR group but not in the NR group, strengthening the validity and utility of this new human cellular model of BD.Molecular Psychiatry advance online publication, 28 February 2017; doi:10.1038/mp.2016.260.

Flynn, J.R., Conn, V.L., Boyle, K.A., Hughes, D.I., Watanabe, M., Velasquez, T., Goulding, M.D., Callister, R.J., Graham, B.A. Anatomical and Molecular Properties of Long Descending Propriospinal Neurons in Mice. (2017) Fronteras en Neuroanatomía. 11:5. DOI: 10.3389/fnana.2017.00005

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Sternfeld, M.J., Hinckley, C.A., Moore, N.J., Pankratz, M.T., Hilde, K.L., Driscoll, S.P., Hayashi, M., Amin, N.D., Bonanomi, D., Gifford, W.D., Sharma, K., Goulding, M., Pfaff, S.L. Speed and segmentation control mechanisms characterized in rhythmically-active circuits created from spinal neurons produced from genetically-tagged embryonic stem cells. (2017) Elife. 6. DOI: 10.7554/eLife.21540

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Tufail, Y., Cook, D., Fourgeaud, L., Powers, C.J., Merten, K., Clark, C.L., Hoffman, E., Ngo, A., Sekiguchi, K.J., O'Shea, C.C., Lemke, G., Nimmerjahn, A. Phosphatidylserine Exposure Controls Viral Innate Immune Responses by Microglia. (2017) Neurona. 93(3):574-586.e8. DOI: 10.1016/j.neuron.2016.12.021

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Adamowicz, D.H., Roy, S., Salmon, D.P., Galasko, D.R., Hansen, L.A., Masliah, E., Gage, F.H. Hippocampal α-Synuclein in Dementia with Lewy Bodies Contributes to Memory Impairment and Is Consistent with Spread of Pathology. (2017) Diario de Neurociencia. 37(7):1675-1684. DOI: 10.1523/JNEUROSCI.3047-16.2016

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Despite considerable research to uncover them, the anatomic and neuropathologic correlates of memory impairment in dementia with Lewy bodies (DLB) remain unclear. While some studies have implicated Lewy bodies in the neocortex, others have pointed to α-synuclein pathology in the hippocampus. We systematically examined hippocampal Lewy pathology and its distribution in hippocampal subfields in 95 clinically and neuropathologically characterized human cases of DLB, finding that α-synuclein pathology was highest in two hippocampal-related subregions: the CA2 subfield and the entorhinal cortex (EC). While the EC had numerous classic somatic Lewy bodies, CA2 contained mainly Lewy neurites in presumed axon terminals, suggesting the involvement of the EC → CA2 circuitry in the pathogenesis of DLB symptoms. Clinicopathological correlations with measures of verbal and visual memory supported a role for EC Lewy pathology, but not CA2, in causing these memory deficits. Lewy pathology in CA1-the main output region for CA2-correlated best with results from memory testing despite a milder pathology. This result indicates that CA1 may be more functionally relevant than CA2 in the context of memory impairment in DLB. These correlations remained significant after controlling for several factors, including concurrent Alzheimer's pathology (neuritic plaques and neurofibrillary tangles) and the interval between time of testing and time of death. Our data suggest that although hippocampal Lewy pathology in DLB is predominant in CA2 and EC, memory performance correlates most strongly with CA1 burden.SIGNIFICANCE STATEMENT This study provides a detailed neuropathologic analysis of hippocampal Lewy pathology in human patients with autopsy-confirmed dementia with Lewy bodies. The approach-informed by regional molecular markers, concurrent Alzheimer's pathology analysis, and relevant clinical data-helps tease out the relative contribution of Lewy pathology to memory dysfunction in the disease. Levels of Lewy pathology were found to be highest in the hippocampal CA2 subregion and entorhinal cortex, implicating a potentially overlooked circuit in disease pathogenesis. However, correlation with memory performance was strongest with CA1. This unexpected finding suggests that Lewy pathology must reach a critical burden across hippocampal circuitry to contribute to memory dysfunction beyond that related to other factors, notably coexisting Alzheimer's disease tau pathology.

Sarkar, A., Marchetto, M.C., Gage, F.H. Synaptic activity: An emerging player in schizophrenia. (2017) Brain Research. 1656:68-75. DOI: 10.1016/j.brainres.2015.12.028

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Paquola, A.C.M., Erwin, J.A., Gage, F.H. Insights into the role of somatic mosaicism in the brain. (2017) Current Opinion in Systems Biology. 1:90-94. DOI: 10.1016/j.coisb.2016.12.004

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Juavinett, A.L., Nauhaus, I., Garrett, M.E., Zhuang, J., Callaway, E.M. Automated identification of mouse visual areas with intrinsic signal imaging. (2017) Nature Protocols. 12(1):32-43. DOI: 10.1038/nprot.2016.158

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O'Donnell, C., Gonçalves, J.T., Whiteley, N., Portera-Cailliau, C., Sejnowski, T.J. The Population Tracking Model: A Simple, Scalable Statistical Model for Neural Population Data. (2017) Computación neuronal. 29(1):50-93. DOI: 10.1162/NECO_a_00910

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Han, J., Gage, F.H. A role for miR-19 in the migration of adult-born neurons and schizophrenia. (2016) Neurogenesis. 3(1):e1251873. DOI: 10.1080/23262133.2016.1251873

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Williams, A.H., O'Donnell, C., Sejnowski, T.J., O'Leary, T. Dendritic trafficking faces physiologically critical speed-precision tradeoffs. (2016) Elife. 5. DOI: 10.7554/eLife.20556

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Sharpee, T.O. Plasticity to the Rescue. (2016) Neurona. 92(5):935-936. DOI: 10.1016/j.neuron.2016.11.042

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Suzuki, K., Tsunekawa, Y., Hernandez-Benitez, R., Wu, J., Zhu, J., Kim, E.J., Hatanaka, F., Yamamoto, M., Araoka, T., Li, Z., Kurita, M., Hishida, T., Li, M., Aizawa, E., Guo, S., Chen, S., Goebl, A., Soligalla, R.D., Qu, J., Jiang, T., Fu, X., Jafari, M., Esteban, C.R., Berggren, W.T., Lajara, J., Nuñez-Delicado, E., Guillen, P., Campistol, J.M., Matsuzaki, F., Liu, G.H., Magistretti, P., Zhang, K., Callaway, E.M., Zhang, K., Belmonte, J.C. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. (2016) Naturaleza. 540(7631):144-149. DOI: 10.1038/nature20565

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Dimidschstein, J., Chen, Q., Tremblay, R., Rogers, S.L., Saldi, G.A., Guo, L., Xu, Q., Liu, R., Lu, C., Chu, J., Grimley, J.S., Krostag, A.R., Kaykas, A., Avery, M.C., Rashid, M.S., Baek, M., Jacob, A.L., Smith, G.B., Wilson, D.E., Kosche, G., Kruglikov, I., Rusielewicz, T., Kotak, V.C., Mowery, T.M., Anderson, S.A., Callaway, E.M., Dasen, J.S., Fitzpatrick, D., Fossati, V., Long, M.A., Noggle, S., Reynolds, J.H., Sanes, D.H., Rudy, B., Feng, G., Fishell, G. A viral strategy for targeting and manipulating interneurons across vertebrate species. (2016) Nature Neuroscience. 19(12):1743-1749. DOI: 10.1038/nn.4430

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Erwin, J.A., Paquola, A.C., Singer, T., Gallina, I., Novotny, M., Quayle, C., Bedrosian, T.A., Alves, F.I., Butcher, C.R., Herdy, J.R., Sarkar, A., Lasken, R.S., Muotri, A.R., Gage, F.H. L1-associated genomic regions are deleted in somatic cells of the healthy human brain. (2016) Nature Neuroscience. 19(12):1583-1591. DOI: 10.1038/nn.4388

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Kuczala, A., Sharpee, T.O. Eigenvalue spectra of large correlated random matrices. (2016) Phys Rev E. 94(5-1):050101. DOI: 10.1103/PhysRevE.94.050101

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Muller, L., Piantoni, G., Koller, D., Cash, S.S., Halgren, E., Sejnowski, T.J. Rotating waves during human sleep spindles organize global patterns of activity that repeat precisely through the night. (2016) Elife. 5. DOI: 10.7554/eLife.17267

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Gonçalves, J.T., Schafer, S.T., Gage, F.H. Adult Neurogenesis in the Hippocampus: From Stem Cells to Behavior. (2016) Móvil. 167(4):897-914. DOI: 10.1016/j.cell.2016.10.021

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Vadodaria, K.C., Marchetto, M.C., Mertens, J., Gage, F.H. Generating human serotonergic neurons in vitro: Methodological advances. (2016) Bioessays. 38(11):1123-1129. DOI: 10.1002/bies.201600127

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Xu, C., Krabbe, S., Gründemann, J., Botta, P., Fadok, J.P., Osakada, F., Saur, D., Grewe, B.F., Schnitzer, M.J., Callaway, E.M., Lüthi, A. Distinct Hippocampal Pathways Mediate Dissociable Roles of Context in Memory Retrieval. (2016) Móvil. 167(4):961-972. DOI: 10.1016/j.cell.2016.09.051

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Peterson, D.A., Littlewort, G.C., Bartlett, M.S., Macerollo, A., Perlmutter, J.S., Jinnah, H.A., Hallett, M., Sejnowski, T.J. Objective, computerized video-based rating of blepharospasm severity. (2016) Neurology. 87(20):2146-2153. DOI: 10.1212/WNL.0000000000003336

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Bardy, C., van den Hurk, M., Kakaradov, B., Erwin, J.A., Jaeger, B.N., Hernandez, R.V., Eames, T., Paucar, A.A., Gorris, M., Marchand, C., Jappelli, R., Barron, J., Bryant, A.K., Kellogg, M., Lasken, R.S., Rutten, B.P., Steinbusch, H.W., Yeo, G.W., Gage, F.H. Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology. (2016) Psiquiatría Molecular. 21(11):1573-1588. DOI: 10.1038/mp.2016.158

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Churchland, P.S., Sejnowski, T.J. Blending computational and experimental neuroscience. (2016) Nature Reviews Neuroscience. 17(11):667-668. DOI: 10.1038/nrn.2016.114

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Tian, J., Huang, R., Cohen, J.Y., Osakada, F., Kobak, D., Machens, C.K., Callaway, E.M., Uchida, N., Watabe-Uchida, M. Distributed and Mixed Information in Monosynaptic Inputs to Dopamine Neurons. (2016) Neurona. 91(6):1374-1389. DOI: 10.1016/j.neuron.2016.08.018

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Barth, L., Burkhalter, A., Callaway, E.M., Connors, B.W., Cauli, B., DeFelipe, J., Feldmeyer, D., Freund, T., Kawaguchi, Y., Kisvarday, Z., Kubota, Y., McBain, C., Oberlaender, M., Rossier, J., Rudy, B., Staiger, J.F., Somogyi, P., Tamas, G., Yuste, R. Comment on "Principles of connectivity among morphologically defined cell types in adult neocortex". (2016) Ciencia. 353(6304):1108. DOI: 10.1126/science.aaf5663

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Du, H., Deng, W., Aimone, J.B., Ge, M., Parylak, S., Walch, K., Zhang, W., Cook, J., Song, H., Wang, L., Gage, F.H., Mu, Y. Dopaminergic inputs in the dentate gyrus direct the choice of memory encoding. (2016) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 113(37):E5501-10. DOI: 10.1073/pnas.1606951113

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Smith, J.B., Klug, J.R., Ross, D.L., Howard, C.D., Hollon, N.G., Ko, V.I., Hoffman, H., Callaway, E.M., Gerfen, C.R., Jin, X. Genetic-Based Dissection Unveils the Inputs and Outputs of Striatal Patch and Matrix Compartments. (2016) Neurona. 91(5):1069-1084. DOI: 10.1016/j.neuron.2016.07.046

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Sharpee, T.O. How Invariant Feature Selectivity Is Achieved in Cortex. (2016) Frente en Neurociencia Sináptica. 8:26. DOI: 10.3389/fnsyn.2016.00026

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Grillner, S., Ip, N., Koch, C., Koroshetz, W., Okano, H., Polachek, M., Poo, M.M., Sejnowski, T.J. Worldwide initiatives to advance brain research. (2016) Nature Neuroscience. 19(9):1118-22. DOI: 10.1038/nn.4371

Kosik, K.S., Sejnowski, T.J., Raichle, M.E., Ciechanover, A., Baltimore, D. A path toward understanding neurodegeneration. (2016) Ciencia. 353(6302):872-3. DOI: 10.1126/science.aai7622

Nauhaus, I., Nielsen, K.J., Callaway, E.M. Efficient Receptive Field Tiling in Primate V1. (2016) Neurona. 91(4):893-904. DOI: 10.1016/j.neuron.2016.07.015

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Reddy, G., Celani, A., Sejnowski, T.J., Vergassola, M. Learning to soar in turbulent environments. (2016) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 113(33):E4877-84. DOI: 10.1073/pnas.1606075113

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Hilde, K.L., Levine, A.J., Hinckley, C.A., Hayashi, M., Montgomery, J.M., Gullo, M., Driscoll, S.P., Grosschedl, R., Kohwi, Y., Kohwi-Shigematsu, T., Pfaff, S.L. Satb2 Is Required for the Development of a Spinal Exteroceptive Microcircuit that Modulates Limb Position. (2016) Neurona. 91(4):763-776. DOI: 10.1016/j.neuron.2016.07.014

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van den Hurk, M., Kenis, G., Bardy, C., van den Hove, D.L., Gage, F.H., Steinbusch, H.W., Rutten, B.P. Transcriptional and epigenetic mechanisms of cellular reprogramming to induced pluripotency. (2016) Epigenomics. 8(8):1131-49. DOI: 10.2217/epi-2016-0032

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Huh, D., Sejnowski, T.J. Conservation law for self-paced movements. (2016) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 113(31):8831-6. DOI: 10.1073/pnas.1608724113

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Gage, F.H., Guarente, L.P., Wagers, A.J. Aging and Rejuvenation: Insights from Rusty Gage, Leonard Guarente, and Amy Wagers. (2016) Tendencias en Medicina Molecular. 22(8):633-4. DOI: 10.1016/j.molmed.2016.06.011

Chailangkarn, T., Trujillo, C.A., Freitas, B.C., Hrvoj-Mihic, B., Herai, R.H., Yu, D.X., Brown, T.T., Marchetto, M.C., Bardy, C., McHenry, L., Stefanacci, L., Järvinen, A., Searcy, Y.M., DeWitt, M., Wong, W., Lai, P., Ard, M.C., Hanson, K.L., Romero, S., Jacobs, B., Dale, A.M., Dai, L., Korenberg, J.R., Gage, F.H., Bellugi, U., Halgren, E., Semendeferi, K., Muotri, A.R. A human neurodevelopmental model for Williams syndrome. (2016) Naturaleza. 536(7616):338-43. DOI: 10.1038/nature19067

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Nuttle, X., Giannuzzi, G., Duyzend, M.H., Schraiber, J.G., Narvaiza, I., Sudmant, P.H., Penn, O., Chiatante, G., Malig, M., Huddleston, J., Benner, C., Camponeschi, F., Ciofi-Baffoni, S., Stessman, H.A., Marchetto, M.C., Denman, L., Harshman, L., Baker, C., Raja, A., Penewit, K., Janke, N., Tang, W.J., Ventura, M., Banci, L., Antonacci, F., Akey, J.M., Amemiya, C.T., Gage, F.H., Reymond, A., Eichler, E.E. Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility. (2016) Naturaleza. 536(7615):205-9. DOI: 10.1038/nature19075

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Padmanabhan, K., Osakada, F., Tarabrina, A., Kizer, E., Callaway, E.M., Gage, F.H., Sejnowski, T.J. Diverse Representations of Olfactory Information in Centrifugal Feedback Projections. (2016) Diario de Neurociencia. 36(28):7535-45. DOI: 10.1523/JNEUROSCI.3358-15.2016

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Ruiz-Martinez, A., Bartol, T.M., Sejnowski, T.J., Tartakovsky, D.M. Efficient Multiscale Models of Polymer Assembly. (2016) Biophysical Journal. 111(1):185-96. DOI: 10.1016/j.bpj.2016.05.022

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Han, J., Kim, H.J., Schafer, S.T., Paquola, A., Clemenson, G.D., Toda, T., Oh, J., Pankonin, A.R., Lee, B.S., Johnston, S.T., Sarkar, A., Denli, A.M., Gage, F.H. Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain. (2016) Neurona. 91(1):79-89. DOI: 10.1016/j.neuron.2016.05.034

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Mertens, J., Marchetto, M.C., Bardy, C., Gage, F.H. Evaluating cell reprogramming, differentiation and conversion technologies in neuroscience. (2016) Nature Reviews Neuroscience. 17(7):424-37. DOI: 10.1038/nrn.2016.46

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Ettle, B., Kerman, B.E., Valera, E., Gillmann, C., Schlachetzki, J.C., Reiprich, S., Büttner, C., Ekici, A.B., Reis, A., Wegner, M., Bäuerle, T., Riemenschneider, M.J., Masliah, E., Gage, F.H., Winkler, J. α-Synuclein-induced myelination deficit defines a novel interventional target for multiple system atrophy. (2016) Acta Neuropathologica. 132(1):59-75. DOI: 10.1007/s00401-016-1572-y

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Multiple system atrophy (MSA) is a rare atypical parkinsonian disorder characterized by a rapidly progressing clinical course and at present without any efficient therapy. Neuropathologically, myelin loss and neurodegeneration are associated with α-synuclein accumulation in oligodendrocytes, but underlying pathomechanisms are poorly understood. Here, we analyzed the impact of oligodendrocytic α-synuclein on the formation of myelin sheaths to define a potential interventional target for MSA. Post-mortem analyses of MSA patients and controls were performed to quantify myelin and oligodendrocyte numbers. As pre-clinical models, we used transgenic MSA mice, a myelinating stem cell-derived oligodendrocyte-neuron co-culture, and primary oligodendrocytes to determine functional consequences of oligodendrocytic α-synuclein overexpression on myelination. We detected myelin loss accompanied by preserved or even increased numbers of oligodendrocytes in post-mortem MSA brains or transgenic mouse forebrains, respectively, indicating an oligodendrocytic dysfunction in myelin formation. Corroborating this observation, overexpression of α-synuclein in primary and stem cell-derived oligodendrocytes severely impaired myelin formation, defining a novel α-synuclein-linked pathomechanism in MSA. We used the pro-myelinating activity of the muscarinic acetylcholine receptor antagonist benztropine to analyze the reversibility of the myelination deficit. Transcriptome profiling of primary pre-myelinating oligodendrocytes demonstrated that benztropine readjusts myelination-related processes such as cholesterol and membrane biogenesis, being compromised by oligodendrocytic α-synuclein. Additionally, benztropine restored the α-synuclein-induced myelination deficit of stem cell-derived oligodendrocytes. Strikingly, benztropine also ameliorated the myelin deficit in transgenic MSA mice, resulting in a prevention of neuronal cell loss. In conclusion, this study defines the α-synuclein-induced myelination deficit as a novel and crucial pathomechanism in MSA. Importantly, the reversible nature of this oligodendrocytic dysfunction opens a novel avenue for an intervention in MSA.

Faget, L., Osakada, F., Duan, J., Ressler, R., Johnson, A.B., Proudfoot, J.A., Yoo, J.H., Callaway, E.M., Hnasko, T.S. Afferent Inputs to Neurotransmitter-Defined Cell Types in the Ventral Tegmental Area. (2016) Cell Reports. 15(12):2796-2808. DOI: 10.1016/j.celrep.2016.05.057

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Zheng, X., Boyer, L., Jin, M., Mertens, J., Kim, Y., Ma, L., Ma, L., Hamm, M., Gage, F.H., Hunter, T. Metabolic reprogramming during neuronal differentiation from aerobic glycolysis to neuronal oxidative phosphorylation. (2016) Elife. 5. DOI: 10.7554/eLife.13374

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Gonçalves, J.T., Bloyd, C.W., Shtrahman, M., Johnston, S.T., Schafer, S.T., Parylak, S.L., Tran, T., Chang, T., Gage, F.H. In vivo imaging of dendritic pruning in dentate granule cells. (2016) Nature Neuroscience. 19(6):788-91. DOI: 10.1038/nn.4301

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Bedrosian, T.A., Linker, S., Gage, F.H. Environment-driven somatic mosaicism in brain disorders. (2016) Genome Medicine. 8(1):58. DOI: 10.1186/s13073-016-0317-9

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Franks, T.M., Benner, C., Narvaiza, I., Marchetto, M.C., Young, J.M., Malik, H.S., Gage, F.H., Hetzer, M.W. Evolución de un regulador transcripcional a partir de un nucleoporina transmembrana. (2016) Genes y Desarrollo. 30(10):1155-71. DOI: 10.1101/gad.280941.116

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Poo, M.M., Pignatelli, M., Ryan, T.J., Tonegawa, S., Bonhoeffer, T., Martin, K.C., Rudenko, A., Tsai, L.H., Tsien, R.W., Fishell, G., Mullins, C., Gonçalves, J.T., Shtrahman, M., Johnston, S.T., Gage, F.H., Dan, Y., Long, J., Buzsáki, G., Stevens, C. What is memory? The present state of the engram. (2016) BMC Biology. 14:40. DOI: 10.1186/s12915-016-0261-6

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Oedegaard, K.J., Alda, M., Anand, A., Andreassen, O.A., Balaraman, Y., Berrettini, W.H., Bhattacharjee, A., Brennand, K.J., Burdick, K.E., Calabrese, J.R., Calkin, C.V., Claasen, A., Coryell, W.H., Craig, D., DeModena, A., Frye, M., Gage, F.H., Gao, K., Garnham, J., Gershon, E., Jakobsen, P., Leckband, S.G., McCarthy, M.J., McInnis, M.G., Maihofer, A.X., Mertens, J., Morken, G., Nievergelt, C.M., Nurnberger, J., Pham, S., Schoeyen, H., Shekhtman, T., Shilling, P.D., Szelinger, S., Tarwater, B., Yao, J., Zandi, P.P., Kelsoe, J.R. The Pharmacogenomics of Bipolar Disorder study (PGBD): identification of genes for lithium response in a prospective sample. (2016) BMC Psychiatry. 16:129. DOI: 10.1186/s12888-016-0732-x

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Topol, A., Zhu, S., Hartley, B.J., English, J., Hauberg, M.E., Tran, N., Rittenhouse, C.A., Simone, A., Ruderfer, D.M., Johnson, J., Readhead, B., Hadas, Y., Gochman, P.A., Wang, Y.C., Shah, H., Cagney, G., Rapoport, J., Gage, F.H., Dudley, J.T., Sklar, P., Mattheisen, M., Cotter, D., Fang, G., Brennand, K.J. Dysregulation of miRNA-9 in a Subset of Schizophrenia Patient-Derived Neural Progenitor Cells. (2016) Cell Reports. 15(5):1024-1036. DOI: 10.1016/j.celrep.2016.03.090

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Jadi, M.P., Behrens, M.M., Sejnowski, T.J. Abnormal Gamma Oscillations in N-Methyl-D-Aspartate Receptor Hypofunction Models of Schizophrenia. (2016) Psiquiatría Biológica. 79(9):716-26. DOI: 10.1016/j.biopsych.2015.07.005

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Saremi, S., Sejnowski, T.J. Correlated Percolation, Fractal Structures, and Scale-Invariant Distribution of Clusters in Natural Images. (2016) IEEE Trans Pattern Anal Mach Intell. 38(5):1016-20. DOI: 10.1109/TPAMI.2015.2481402

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Kim, E.J., Jacobs, M.W., Ito-Cole, T., Callaway, E.M. Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins. (2016) Cell Reports. 15(4):692-699. DOI: 10.1016/j.celrep.2016.03.067

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Sharpee, T.O. Taking a close look at electrosensing. (2016) Elife. 5. DOI: 10.7554/eLife.16209

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Sekiguchi, K.J., Shekhtmeyster, P., Merten, K., Arena, A., Cook, D., Hoffman, E., Ngo, A., Nimmerjahn, A. Imaging large-scale cellular activity in spinal cord of freely behaving mice. (2016) Nature Communications. 7:11450. DOI: 10.1038/ncomms11450

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Lacar, B., Linker, S.B., Jaeger, B.N., Krishnaswami, S., Barron, J., Kelder, M., Parylak, S., Paquola, A., Venepally, P., Novotny, M., O'Connor, C., Fitzpatrick, C., Erwin, J., Hsu, J.Y., Husband, D., McConnell, M.J., Lasken, R., Gage, F.H. Nuclear RNA-seq of single neurons reveals molecular signatures of activation. (2016) Nature Communications. 7:11022. DOI: 10.1038/ncomms11022

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Harrison, B.J., Venkat, G., Lamb, J.L., Hutson, T.H., Drury, C., Rau, K.K., Bunge, M.B., Mendell, L.M., Gage, F.H., Johnson, R.D., Hill, C.E., Rouchka, E.C., Moon, L.D., Petruska, J.C. The Adaptor Protein CD2AP Is a Coordinator of Neurotrophin Signaling-Mediated Axon Arbor Plasticity. (2016) Diario de Neurociencia. 36(15):4259-75. DOI: 10.1523/JNEUROSCI.2423-15.2016

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Zhang, Y., Sharpee, T.O. A Robust Feedforward Model of the Olfactory System. (2016) PLOS Biología Computacional. 12(4):e1004850. DOI: 10.1371/journal.pcbi.1004850

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Wall, N.R., De La Parra, M., Sorokin, J.M., Taniguchi, H., Huang, Z.J., Callaway, E.M. Brain-Wide Maps of Synaptic Input to Cortical Interneurons. (2016) Diario de Neurociencia. 36(14):4000-4009. DOI: 10.1523/JNEUROSCI.3967-15.2016

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Fourgeaud, L., Través, P.G., Tufail, Y., Leal-Bailey, H., Lew, E.D., Burrola, P.G., Callaway, P., Zagórska, A., Rothlin, C.V., Nimmerjahn, A., Lemke, G. TAM receptors regulate multiple features of microglial physiology. (2016) Naturaleza. 532(7598):240-244. DOI: 10.1038/nature17630

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Otani, T., Marchetto, M.C., Gage, F.H., Simons, B.D., Livesey, F.J. 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. (2016) Células Madre. 18(4):467-80. DOI: 10.1016/j.stem.2016.03.003

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Briggs, F., Kiley, C.W., Callaway, E.M., Usrey, W.M. Morphological Substrates for Parallel Streams of Corticogeniculate Feedback Originating in Both V1 and V2 of the Macaque Monkey. (2016) Neurona. 90(2):388-399. DOI: 10.1016/j.neuron.2016.02.038

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Zheng, X., Boyer, L., Jin, M., Kim, Y., Fan, W., Bardy, C., Berggren, T., Evans, R.M., Gage, F.H., Hunter, T. Alleviation of neuronal energy deficiency by mTOR inhibition as a treatment for mitochondria-related neurodegeneration. (2016) Elife. 5. DOI: 10.7554/eLife.13378

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Mishra, H.K., Prots, I., Havlicek, S., Kohl, Z., Perez-Branguli, F., Boerstler, T., Anneser, L., Minakaki, G., Wend, H., Hampl, M., Leone, M., Brückner, M., Klucken, J., Reis, A., Boyer, L., Schuierer, G., Behrens, J., Lampert, A., Engel, F.B., Gage, F.H., Winkler, J., Winner, B. GSK3ß-dependent dysregulation of neurodevelopment in SPG11-patient iPSC model. (2016) Ann. Neurol. DOI: 10.1002/ana.24633

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Krishnaswami, S.R., Grindberg, R.V., Novotny, M., Venepally, P., Lacar, B., Bhutani, K., Linker, S.B., Pham, S., Erwin, J.A., Miller, J.A., Hodge, R., McCarthy, J.K., Kelder, M., McCorrison, J., Aevermann, B.D., Fuertes, F.D., Scheuermann, R.H., Lee, J., Lein, E.S., Schork, N., McConnell, M.J., Gage, F.H., Lasken, R.S. Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons. (2016) Nature Protocols. 11(3):499-524. DOI: 10.1038/nprot.2016.015

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Johnston, S.T., Shtrahman, M., Parylak, S., Gonçalves, J.T., Gage, F.H. Paradox of pattern separation and adult neurogenesis: A dual role for new neurons balancing memory resolution and robustness. (2016) Neurobiology of Learning and Memory. 129:60-8. DOI: 10.1016/j.nlm.2015.10.013

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Zarrinpar, A., Callaway, E.M. Functional Local Input to Layer 5 Pyramidal Neurons in the Rat Visual Cortex. (2016) Cereb. Cortex. 26(3):991-1003. DOI: 10.1093/cercor/bhu268

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Aljadeff, J., Renfrew, D., Vegué, M., Sharpee, T.O. Low-dimensional dynamics of structured random networks. (2016) Phys Rev E. 93(2):022302. DOI: 10.1103/PhysRevE.93.022302

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Weissman, I.L., Gage, F.H. A Mechanism for Somatic Brain Mosaicism. (2016) Móvil. 164(4):593-5. DOI: 10.1016/j.cell.2016.01.048

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Rodrigues, S., Desroches, M., Krupa, M., Cortes, J.M., Sejnowski, T.J., Ali, A.B. Time-coded neurotransmitter release at excitatory and inhibitory synapses. (2016) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 113(8):E1108-15. DOI: 10.1073/pnas.1525591113

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Tuncdemir, S.N., Wamsley, B., Stam, F.J., Osakada, F., Goulding, M., Callaway, E.M., Rudy, B., Fishell, G. Early Somatostatin Interneuron Connectivity Mediates the Maturation of Deep Layer Cortical Circuits. (2016) Neurona. 89(3):521-35. DOI: 10.1016/j.neuron.2015.11.020

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Hasenstaub, A., Otte, S., Callaway, E. Cell Type-Specific Control of Spike Timing by Gamma-Band Oscillatory Inhibition. (2016) Cereb. Cortex. 26(2):797-806. DOI: 10.1093/cercor/bhv044

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Agre, P., Bertozzi, C., Bissell, M., Campbell, K.P., Cummings, R.D., Desai, U.R., Estes, M., Flotte, T., Fogleman, G., Gage, F., Ginsburg, D., Gordon, J.I., Hart, G., Hascall, V., Kiessling, L., Kornfeld, S., Lowe, J., Magnani, J., Mahal, L.K., Medzhitov, R., Roberts, R.J., Sackstein, R., Sarkar, R., Schnaar, R., Schwartz, N., Varki, A., Walt, D., Weissman, I. Training the next generation of biomedical investigators in glycosciences. (2016) Revista de Investigación Clínica. 126(2):405-8. DOI: 10.1172/JCI85905

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Wasson, J.A., Simon, A.K., Myrick, D.A., Wolf, G., Driscoll, S., Pfaff, S.L., Macfarlan, T.S., Katz, D.J. Maternally provided LSD1/KDM1A enables the maternal-to-zygotic transition and prevents defects that manifest postnatally. (2016) Elife. 5. DOI: 10.7554/eLife.08848

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O'Donnell, C., Sejnowski, T.J. Street View of the Cognitive Map. (2016) Móvil. 164(1-2):13-5. DOI: 10.1016/j.cell.2015.12.051

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Tang, X., Kim, J., Zhou, L., Wengert, E., Zhang, L., Wu, Z., Carromeu, C., Muotri, A.R., Marchetto, M.C., Gage, F.H., Chen, G. KCC2 rescues functional deficits in human neurons derived from patients with Rett syndrome. (2016) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 113(3):751-6. DOI: 10.1073/pnas.1524013113

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Vadodaria, K.C., Mertens, J., Paquola, A., Bardy, C., Li, X., Jappelli, R., Fung, L., Marchetto, M.C., Hamm, M., Gorris, M., Koch, P., Gage, F.H. The different moods of human serotonergic neurons. (2016) Psiquiatría Molecular. 21(1):3. DOI: 10.1038/mp.2015.202

Duricki, D.A., Hutson, T.H., Kathe, C., Soleman, S., Gonzalez-Carter, D., Petruska, J.C., Shine, H.D., Chen, Q., Wood, T.C., Bernanos, M., Cash, D., Williams, S.C., Gage, F.H., Moon, L.D. Delayed intramuscular human neurotrophin-3 improves recovery in adult and elderly rats after stroke. (2016) Brain. 139(Pt 1):259-75. DOI: 10.1093/brain/awv341

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Vadodaria, K.C., Mertens, J., Paquola, A., Bardy, C., Li, X., Jappelli, R., Fung, L., Marchetto, M.C., Hamm, M., Gorris, M., Koch, P., Gage, F.H. Generation of functional human serotonergic neurons from fibroblasts. (2016) Psiquiatría Molecular. 21(1):49-61. DOI: 10.1038/mp.2015.161

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Geeven, G., Zhu, Y., Kim, B.J., Bartholdy, B.A., Yang, S.M., Macfarlan, T.S., Gifford, W.D., Pfaff, S.L., Verstegen, M.J., Pinto, H., Vermunt, M.W., Creyghton, M.P., Wijchers, P.J., Stamatoyannopoulos, J.A., Skoultchi, A.I., de Laat, W. Local compartment changes and regulatory landscape alterations in histone H1-depleted cells. (2015) Biología del Genoma. 16:289. DOI: 10.1186/s13059-015-0857-0

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Harrison, B.J., Venkat, G., Hutson, T., Rau, K.K., Bunge, M.B., Mendell, L.M., Gage, F.H., Johnson, R.D., Hill, C., Rouchka, E.C., Moon, L., Petruska, J.C. Transcriptional changes in sensory ganglia associated with primary afferent axon collateral sprouting in spared dermatome model. (2015) Genomics Data. 6:249-52. DOI: 10.1016/j.gdata.2015.10.005

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Amin, N.D., Bai, G., Klug, J.R., Bonanomi, D., Pankratz, M.T., Gifford, W.D., Hinckley, C.A., Sternfeld, M.J., Driscoll, S.P., Dominguez, B., Lee, K.F., Jin, X., Pfaff, S.L. Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure. (2015) Ciencia. 350(6267):1525-9. DOI: 10.1126/science.aad2509

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Kim, E.J., Juavinett, A.L., Kyubwa, E.M., Jacobs, M.W., Callaway, E.M. Three Types of Cortical Layer 5 Neurons That Differ in Brain-wide Connectivity and Function. (2015) Neurona. 88(6):1253-1267. DOI: 10.1016/j.neuron.2015.11.002

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Brennand, K.J., Marchetto, M.C., Benvenisty, N., Brüstle, O., Ebert, A., Izpisua Belmonte, J.C., Kaykas, A., Lancaster, M.A., Livesey, F.J., McConnell, M.J., McKay, R.D., Morrow, E.M., Muotri, A.R., Panchision, D.M., Rubin, L.L., Sawa, A., Soldner, F., Song, H., Studer, L., Temple, S., Vaccarino, F.M., Wu, J., Vanderhaeghen, P., Gage, F.H., Jaenisch, R. Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders. (2015) Stem Cell Reports. 5(6):933-45. DOI: 10.1016/j.stemcr.2015.10.011

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Veltz, R., Sejnowski, T.J. Periodic Forcing of Inhibition-Stabilized Networks: Nonlinear Resonances and Phase-Amplitude Coupling. (2015) Computación neuronal. 27(12):2477-509. DOI: 10.1162/NECO_a_00786

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Mertens, J., Paquola, A.C., Ku, M., Hatch, E., Böhnke, L., Ladjevardi, S., McGrath, S., Campbell, B., Lee, H., Herdy, J.R., Gonçalves, J.T., Toda, T., Kim, Y., Winkler, J., Yao, J., Hetzer, M.W., Gage, F.H. Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects. (2015) Células Madre. 17(6):705-18. DOI: 10.1016/j.stem.2015.09.001

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Bartol, T.M., Bromer, C., Kinney, J., Chirillo, M.A., Bourne, J.N., Harris, K.M., Sejnowski, T.J. Nanoconnectomic upper bound on the variability of synaptic plasticity. (2015) Elife. 4:e10778. DOI: 10.7554/eLife.10778

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Kim, H.J., Denli, A.M., Wright, R., Baul, T.D., Clemenson, G.D., Morcos, A.S., Zhao, C., Schafer, S.T., Gage, F.H., Kagalwala, M.N. REST Regulates Non-Cell-Autonomous Neuronal Differentiation and Maturation of Neural Progenitor Cells via Secretogranin II. (2015) Diario de Neurociencia. 35(44):14872-84. DOI: 10.1523/JNEUROSCI.4286-14.2015

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Pan, F.C., Brissova, M., Powers, A.C., Pfaff, S., Wright, C.V. Inactivating the permanent neonatal diabetes gene Mnx1 switches insulin-producing β-cells to a δ-like fate and reveals a facultative proliferative capacity in aged β-cells. (2015) Development. 142(21):3637-48. DOI: 10.1242/dev.126011

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Mertens, J., Wang, Q.W., Kim, Y., Yu, D.X., Pham, S., Yang, B., Zheng, Y., Diffenderfer, K.E., Zhang, J., Soltani, S., Eames, T., Schafer, S.T., Boyer, L., Marchetto, M.C., Nurnberger, J.I., Calabrese, J.R., Ødegaard, K.J., McCarthy, M.J., Zandi, P.P., Alda, M., Alba, M., Nievergelt, C.M., , Mi, S., Brennand, K.J., Kelsoe, J.R., Gage, F.H., Yao, J. Differential responses to lithium in hyperexcitable neurons from patients with bipolar disorder. (2015) Naturaleza. 527(7576):95-9. DOI: 10.1038/nature15526

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Bipolar disorder is a complex neuropsychiatric disorder that is characterized by intermittent episodes of mania and depression; without treatment, 15% of patients commit suicide. Hence, it has been ranked by the World Health Organization as a top disorder of morbidity and lost productivity. Previous neuropathological studies have revealed a series of alterations in the brains of patients with bipolar disorder or animal models, such as reduced glial cell number in the prefrontal cortex of patients, upregulated activities of the protein kinase A and C pathways and changes in neurotransmission. However, the roles and causation of these changes in bipolar disorder have been too complex to exactly determine the pathology of the disease. Furthermore, although some patients show remarkable improvement with lithium treatment for yet unknown reasons, others are refractory to lithium treatment. Therefore, developing an accurate and powerful biological model for bipolar disorder has been a challenge. The introduction of induced pluripotent stem-cell (iPSC) technology has provided a new approach. Here we have developed an iPSC model for human bipolar disorder and investigated the cellular phenotypes of hippocampal dentate gyrus-like neurons derived from iPSCs of patients with bipolar disorder. Guided by RNA sequencing expression profiling, we have detected mitochondrial abnormalities in young neurons from patients with bipolar disorder by using mitochondrial assays; in addition, using both patch-clamp recording and somatic Ca(2+) imaging, we have observed hyperactive action-potential firing. This hyperexcitability phenotype of young neurons in bipolar disorder was selectively reversed by lithium treatment only in neurons derived from patients who also responded to lithium treatment. Therefore, hyperexcitability is one early endophenotype of bipolar disorder, and our model of iPSCs in this disease might be useful in developing new therapies and drugs aimed at its clinical treatment.

Bourane, S., Duan, B., Koch, S.C., Dalet, A., Britz, O., Garcia-Campmany, L., Kim, E., Cheng, L., Ghosh, A., Ma, Q., Goulding, M. Gate control of mechanical itch by a subpopulation of spinal cord interneurons. (2015) Ciencia. 350(6260):550-4. DOI: 10.1126/science.aac8653

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He, W., Bai, G., Zhou, H., Wei, N., White, N.M., Lauer, J., Liu, H., Shi, Y., Dumitru, C.D., Lettieri, K., Shubayev, V., Jordanova, A., Guergueltcheva, V., Griffin, P.R., Burgess, R.W., Pfaff, S.L., Yang, X.L. CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase. (2015) Naturaleza. 526(7575):710-4. DOI: 10.1038/nature15510

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Bartol, T.M., Keller, D.X., Kinney, J.P., Bajaj, C.L., Harris, K.M., Sejnowski, T.J., Kennedy, M.B. Computational reconstitution of spine calcium transients from individual proteins. (2015) Frente en Neurociencia Sináptica. 7:17. DOI: 10.3389/fnsyn.2015.00017

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Denli, A.M., Narvaiza, I., Kerman, B.E., Pena, M., Benner, C., Marchetto, M.C., Diedrich, J.K., Aslanian, A., Ma, J., Moresco, J.J., Moore, L., Hunter, T., Saghatelian, A., Gage, F.H. Primate-specific ORF0 contributes to retrotransposon-mediated diversity. (2015) Móvil. 163(3):583-93. DOI: 10.1016/j.cell.2015.09.025

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Mu, Y., Zhao, C., Toni, N., Yao, J., Gage, F.H. Distinct roles of NMDA receptors at different stages of granule cell development in the adult brain. (2015) Elife. 4:e07871. DOI: 10.7554/eLife.07871

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Britz, O., Zhang, J., Grossmann, K.S., Dyck, J., Kim, J.C., Dymecki, S., Gosgnach, S., Goulding, M. A genetically defined asymmetry underlies the inhibitory control of flexor-extensor locomotor movements. (2015) Elife. 4. DOI: 10.7554/eLife.04718

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Wang, X., Pinto-Duarte, A., Behrens, M.M., Zhou, X., Sejnowski, T.J. Characterization of spatio-temporal epidural event-related potentials for mouse models of psychiatric disorders. (2015) Scientific Reports. 5:14964. DOI: 10.1038/srep14964

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Barnes, S.A., Pinto-Duarte, A., Kappe, A., Zembrzycki, A., Metzler, A., Mukamel, E.A., Lucero, J., Wang, X., Sejnowski, T.J., Markou, A.*, Behrens, M.M.* Disruption of mGluR5 in parvalbumin-positive interneurons induces core features of neurodevelopmental disorders. (2015) Psiquiatría Molecular. 20(10):1161-72. DOI: 10.1038/mp.2015.113

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Pawar, K., Cummings, B.J., Thomas, A., Shea, L.D., Levine, A., Pfaff, S., Anderson, A.J. Biomaterial bridges enable regeneration and re-entry of corticospinal tract axons into the caudal spinal cord after SCI: Association with recovery of forelimb function. (2015) Biomaterials. 65:1-12. DOI: 10.1016/j.biomaterials.2015.05.032

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Tashiro, A., Zhao, C., Suh, H., Gage, F.H. Preparation and Use of Retroviral Vectors for Labeling, Imaging, and Genetically Manipulating Cells. (2015) Protocolos de Cold Spring Harbor. 2015(10):883-8. DOI: 10.1101/pdb.top086363

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Tashiro, A., Zhao, C., Suh, H., Gage, F.H. Analysis of Spine Motility of Newborn Granule Cells in Acute Brain Slices. (2015) Protocolos de Cold Spring Harbor. 2015(10):934-6. DOI: 10.1101/pdb.prot086397

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Tashiro, A., Zhao, C., Suh, H., Gage, F.H. Imaging Newborn Granule Cells in Fixed Sections. (2015) Protocolos de Cold Spring Harbor. 2015(10):932-3. DOI: 10.1101/pdb.prot086389

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Tashiro, A., Zhao, C., Suh, H., Gage, F.H. Purification and Injection of Retroviral Vectors. (2015) Protocolos de Cold Spring Harbor. 2015(10):925-31. DOI: 10.1101/pdb.prot086371

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González, O.C., Krishnan, G.P., Chauvette, S., Timofeev, I., Sejnowski, T., Bazhenov, M. Modeling of Age-Dependent Epileptogenesis by Differential Homeostatic Synaptic Scaling. (2015) Diario de Neurociencia. 35(39):13448-62. DOI: 10.1523/JNEUROSCI.5038-14.2015

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Prescott, S.L., Srinivasan, R., Marchetto, M.C., Grishina, I., Narvaiza, I., Selleri, L., Gage, F.H., Swigut, T., Wysocka, J. Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest. (2015) Móvil. 163(1):68-83. DOI: 10.1016/j.cell.2015.08.036

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Hinckley, C.A., Alaynick, W.A., Gallarda, B.W., Hayashi, M., Hilde, K.L., Driscoll, S.P., Dekker, J.D., Tucker, H.O., Sharpee, T.O., Pfaff, S.L. Spinal Locomotor Circuits Develop Using Hierarchical Rules Based on Motorneuron Position and Identity. (2015) Neurona. 87(5):1008-21. DOI: 10.1016/j.neuron.2015.08.005

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Kempermann, G., Song, H., Gage, F.H. Neurogenesis in the Adult Hippocampus. (2015) Perspectivas de Cold Spring Harbor en Biología. 7(9):a018812. DOI: 10.1101/cshperspect.a018812

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Jovičić, A., Mertens, J., Boeynaems, S., Bogaert, E., Chai, N., Yamada, S.B., Paul, J.W., Sun, S., Herdy, J.R., Bieri, G., Kramer, N.J., Gage, F.H., Van Den Bosch, L., Robberecht, W., Gitler, A.D. Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. (2015) Nature Neuroscience. 18(9):1226-9. DOI: 10.1038/nn.4085

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Firth, A.L., Menon, T., Parker, G.S., Qualls, S.J., Lewis, B.M., Ke, E., Dargitz, C.T., Wright, R., Khanna, A., Gage, F.H., Verma, I.M. Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs. (2015) Cell Reports. 12(9):1385-90. DOI: 10.1016/j.celrep.2015.07.062

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Wang, J., Telese, F., Tan, Y., Li, W., Jin, C., He, X., Basnet, H., Ma, Q., Merkurjev, D., Zhu, X., Liu, Z., Zhang, J., Ohgi, K., Taylor, H., White, R.R., Tazearslan, C., Suh, Y., Macfarlan, T.S., Pfaff, S.L., Rosenfeld, M.G. LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control. (2015) Nature Neuroscience. 18(9):1256-64. DOI: 10.1038/nn.4069

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Deng, W., Gage, F.H. The effect of immature adult-born dentate granule cells on hyponeophagial behavior is related to their roles in learning and memory. (2015) Frontiers in Systems Neuroscience. 9:34. DOI: 10.3389/fnsys.2015.00034

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Coggan, J.S., Sejnowski, T.J., Prescott, S.A. Cooperativity between remote sites of ectopic spiking allows afterdischarge to be initiated and maintained at different locations. (2015) Journal of Computational Neuroscience. 39(1):17-28. DOI: 10.1007/s10827-015-0562-8

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Huh, D., Sejnowski, T.J. Spectrum of power laws for curved hand movements. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(29):E3950-8. DOI: 10.1073/pnas.1510208112

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Han, J., Sarkar, A., Gage, F.H. MIR137: big impacts from small changes. (2015) Nature Neuroscience. 18(7):931-3. DOI: 10.1038/nn.4045

Schultz, M.D., He, Y., Whitaker, J.W., Hariharan, M., Mukamel, E.A., Leung, D., Rajagopal, N., Nery, J.R., Urich, M.A., Chen, H., Lin, S., Lin, Y., Jung, I., Schmitt, A.D., Selvaraj, S., Ren, B., Sejnowski, T.J., Wang, W., Ecker, J.R. Human body epigenome maps reveal noncanonical DNA methylation variation. (2015) Naturaleza. 523(7559):212-6. DOI: 10.1038/nature14465

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Lainscsek, C., Weyhenmeyer, J., Sejnowski, T.J., Letellier, C. Discovering independent parameters in complex dynamical systems. (2015) Chaos Solitons Fractals. 76:182-189. DOI: 10.1016/j.chaos.2015.04.001

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Lee, H., Kim, M., Kim, N., Macfarlan, T., Pfaff, S.L., Mastick, G.S., Song, M.R. Slit and Semaphorin signaling governed by Islet transcription factors positions motor neuron somata within the neural tube. (2015) Experimental Neurology. 269:17-27. DOI: 10.1016/j.expneurol.2015.03.024

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Zhao, C., Jou, J., Wolff, L.J., Sun, H., Gage, F.H. Spine morphogenesis in newborn granule cells is differentially regulated in the outer and middle molecular layers. (2015) Journal of Comparative Neurology. 523(10):1588. DOI: 10.1002/cne.23800

Krzisch, M., Temprana, S.G., Mongiat, L.A., Armida, J., Schmutz, V., Virtanen, M.A., Kocher-Braissant, J., Kraftsik, R., Vutskits, L., Conzelmann, K.K., Bergami, M., Gage, F.H., Schinder, A.F., Toni, N. Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus. (2015) Brain Structure & Function. 220(4):2027-42. DOI: 10.1007/s00429-014-0768-y

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Mo, A., Mukamel, E.A., Davis, F.P., Luo, C., Henry, G.L., Picard, S., Urich, M.A., Nery, J.R., Sejnowski, T.J., Lister, R., Eddy, S.R., Ecker, J.R., Nathans, J. Epigenomic Signatures of Neuronal Diversity in the Mammalian Brain. (2015) Neurona. 86(6):1369-84. DOI: 10.1016/j.neuron.2015.05.018

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Callaway, E.M., Luo, L. Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses. (2015) Diario de Neurociencia. 35(24):8979-8985. DOI: 10.1523/JNEUROSCI.0409-15.2015

Juavinett, A.L., Callaway, E.M. Pattern and Component Motion Responses in Mouse Visual Cortical Areas. (2015) Biología Actual. 25(13):1759-1764. DOI: 10.1016/j.cub.2015.05.028

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Kerman, B.E., Kim, H.J., Padmanabhan, K., Mei, A., Georges, S., Joens, M.S., Fitzpatrick, J.A., Jappelli, R., Chandross, K.J., August, P., Gage, F.H. In vitro myelin formation using embryonic stem cells. (2015) Development. 142(12):2213-25. DOI: 10.1242/dev.116517

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Nimmerjahn, A., Bergles, D.E. Large-scale recording of astrocyte activity. (2015) Current Opinion in Neurobiology. 32:95-106. DOI: 10.1016/j.conb.2015.01.015

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Johnson, T., Bartol, T., Sejnowski, T., Mjolsness, E. Model reduction for stochastic CaMKII reaction kinetics in synapses by graph-constrained correlation dynamics. (2015) Phys Biol. 12(4):045005. DOI: 10.1088/1478-3975/12/4/045005

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Mukherjee, S., Hsieh, W.L., Smith, N., Goulding, M., Heikenfeld, J. Electrokinetic pixels with biprimary inks for color displays and color-temperature-tunable smart windows. (2015) Appl Opt. 54(17):5603-5609.

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Sejnowski, T.J. Vernon Mountcastle: Father of neuroscience. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(21):6523-4. DOI: 10.1073/pnas.1506644112

Izpisua Belmonte, J.C., Callaway, E.M., Caddick, S.J., Churchland, P., Feng, G., Homanics, G.E., Lee, K.F., Leopold, D.A., Miller, C.T., Mitchell, J.F., Mitalipov, S., Moutri, A.R., Movshon, J.A., Okano, H., Reynolds, J.H., Ringach, D., Sejnowski, T.J., Silva, A.C., Strick, P.L., Wu, J., Zhang, F. Brains, genes, and primates. (2015) Neurona. 86(3):617-31. DOI: 10.1016/j.neuron.2015.03.021

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Bardy, C., van den Hurk, M., Eames, T., Marchand, C., Hernandez, R.V., Kellogg, M., Gorris, M., Galet, B., Palomares, V., Brown, J., Bang, A.G., Mertens, J., Böhnke, L., Boyer, L., Simon, S., Gage, F.H. Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(20):E2725-34. DOI: 10.1073/pnas.1504393112

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Jorgenson, L.A., Newsome, W.T., Anderson, D.J., Bargmann, C.I., Brown, E.N., Deisseroth, K., Donoghue, J.P., Hudson, K.L., Ling, G.S., MacLeish, P.R., Marder, E., Normann, R.A., Sanes, J.R., Schnitzer, M.J., Sejnowski, T.J., Tank, D.W., Tsien, R.Y., Ugurbil, K., Wingfield, J.C. The BRAIN Initiative: developing technology to catalyse neuroscience discovery. (2015) Philos. Trans. R. Soc. Lond., B, Biol. Sci. 370(1668). DOI: 10.1098/rstb.2014.0164

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Calhoun, A.J., Tong, A., Pokala, N., Fitzpatrick, J.A., Sharpee, T.O., Chalasani, S.H. Neural Mechanisms for Evaluating Environmental Variability in Caenorhabditis elegans. (2015) Neurona. 86(2):428-41. DOI: 10.1016/j.neuron.2015.03.026

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Pei, L., Mu, Y., Leblanc, M., Alaynick, W., Barish, G.D., Pankratz, M., Tseng, T.W., Kaufman, S., Liddle, C., Yu, R.T., Downes, M., Pfaff, S.L., Auwerx, J., Gage, F.H., Evans, R.M. Dependence of hippocampal function on ERRγ-regulated mitochondrial metabolism. (2015) Metabolismo Celular. 21(4):628-36. DOI: 10.1016/j.cmet.2015.03.004

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Adamowicz, D.H., Mertens, J., Gage, F.H. Alzheimer's disease: distinct stages in neurogenic decline? (2015) Psiquiatría Biológica. 77(8):680-2. DOI: 10.1016/j.biopsych.2015.02.021

Amador-Arjona, A., Cimadamore, F., Huang, C.T., Wright, R., Lewis, S., Gage, F.H., Terskikh, A.V. SOX2 primes the epigenetic landscape in neural precursors enabling proper gene activation during hippocampal neurogenesis. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(15):E1936-45. DOI: 10.1073/pnas.1421480112

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Schafer, S.T., Han, J., Pena, M., von Bohlen Und Halbach, O., Peters, J., Gage, F.H. The Wnt adaptor protein ATP6AP2 regulates multiple stages of adult hippocampal neurogenesis. (2015) Diario de Neurociencia. 35(12):4983-98. DOI: 10.1523/JNEUROSCI.4130-14.2015

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Li, P.H., Gauthier, J.L., Schiff, M., Sher, A., Ahn, D., Field, G.D., Greschner, M., Callaway, E.M., Litke, A.M., Chichilnisky, E.J. Anatomical identification of extracellularly recorded cells in large-scale multielectrode recordings. (2015) Diario de Neurociencia. 35(11):4663-4675. DOI: 10.1523/JNEUROSCI.3675-14.2015

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Lainscsek, C., Sejnowski, T.J. Delay differential analysis of time series. (2015) Computación neuronal. 27(3):594-614. DOI: 10.1162/NECO_a_00706

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Nonlinear dynamical system analysis based on embedding theory has been used for modeling and prediction, but it also has applications to signal detection and classification of time series. An embedding creates a multidimensional geometrical object from a single time series. Traditionally either delay or derivative embeddings have been used. The delay embedding is composed of delayed versions of the signal, and the derivative embedding is composed of successive derivatives of the signal. The delay embedding has been extended to nonuniform embeddings to take multiple timescales into account. Both embeddings provide information on the underlying dynamical system without having direct access to all the system variables. Delay differential analysis is based on functional embeddings, a combination of the derivative embedding with nonuniform delay embeddings. Small delay differential equation (DDE) models that best represent relevant dynamic features of time series data are selected from a pool of candidate models for detection or classification. We show that the properties of DDEs support spectral analysis in the time domain where nonlinear correlation functions are used to detect frequencies, frequency and phase couplings, and bispectra. These can be efficiently computed with short time windows and are robust to noise. For frequency analysis, this framework is a multivariate extension of discrete Fourier transform (DFT), and for higher-order spectra, it is a linear and multivariate alternative to multidimensional fast Fourier transform of multidimensional correlations. This method can be applied to short or sparse time series and can be extended to cross-trial and cross-channel spectra if multiple short data segments of the same experiment are available. Together, this time-domain toolbox provides higher temporal resolution, increased frequency and phase coupling information, and it allows an easy and straightforward implementation of higher-order spectra across time compared with frequency-based methods such as the DFT and cross-spectral analysis.

Clemenson, G.D., Lee, S.W., Deng, W., Barrera, V.R., Iwamoto, K.S., Fanselow, M.S., Gage, F.H. Enrichment rescues contextual discrimination deficit associated with immediate shock. (2015) Hipocampo. 25(3):385-92. DOI: 10.1002/hipo.22380

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Lainscsek, C., Hernandez, M.E., Poizner, H., Sejnowski, T.J. Delay differential analysis of electroencephalographic data. (2015) Computación neuronal. 27(3):615-27. DOI: 10.1162/NECO_a_00656

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Brennand, K., Savas, J.N., Kim, Y., Tran, N., Simone, A., Hashimoto-Torii, K., Beaumont, K.G., Kim, H.J., Topol, A., Ladran, I., Abdelrahim, M., Matikainen-Ankney, B., Chao, S.H., Mrksich, M., Rakic, P., Fang, G., Zhang, B., Yates, J.R., Gage, F.H. Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. (2015) Psiquiatría Molecular. 20(3):361-8. DOI: 10.1038/mp.2014.22

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Aljadeff, J., Stern, M., Sharpee, T. Transition to chaos in random networks with cell-type-specific connectivity. (2015) Phys. Rev. Lett. 114(8):088101. DOI: 10.1103/PhysRevLett.114.088101

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Moldakarimov, S., Bazhenov, M., Sejnowski, T.J. Feedback stabilizes propagation of synchronous spiking in cortical neural networks. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(8):2545-50. DOI: 10.1073/pnas.1500643112

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Kastner, D.B., Baccus, S.A., Sharpee, T.O. Critical and maximally informative encoding between neural populations in the retina. (2015) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 112(8):2533-8. DOI: 10.1073/pnas.1418092112

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Tanaka, H., Sejnowski, T.J. Motor adaptation and generalization of reaching movements using motor primitives based on spatial coordinates. (2015) Journal of Neurophysiology. 113(4):1217-33. DOI: 10.1152/jn.00002.2014

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Frost, W.N., Brandon, C.J., Bruno, A.M., Humphries, M.D., Moore-Kochlacs, C., Sejnowski, T.J., Wang, J., Hill, E.S. Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia. (2015) Advances in Experimental Medicine and Biology. 859:127-45. DOI: 10.1007/978-3-319-17641-3_5

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Sejnowski, T.J. Consciousness. (2015) Daedalus. 144(1):123-132. DOI: 10.1162/DAED_a_00321

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Bourane, S., Grossmann, K.S., Britz, O., Dalet, A., Del Barrio, M.G., Stam, F.J., Garcia-Campmany, L., Koch, S., Goulding, M. Identification of a spinal circuit for light touch and fine motor control. (2015) Móvil. 160(3):503-15. DOI: 10.1016/j.cell.2015.01.011

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Duan, B., Cheng, L., Bourane, S., Britz, O., Padilla, C., Garcia-Campmany, L., Krashes, M., Knowlton, W., Velasquez, T., Ren, X., Ross, S., Lowell, B.B., Wang, Y., Goulding, M., Ma, Q. Identification of spinal circuits transmitting and gating mechanical pain. (2014) Móvil. 159(6):1417-1432. DOI: 10.1016/j.cell.2014.11.003

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Ritaccio, A., Brunner, P., Gunduz, A., Hermes, D., Hirsch, L.J., Jacobs, J., Kamada, K., Kastner, S., Knight, R.T., Lesser, R.P., Miller, K., Sejnowski, T., Worrell, G., Schalk, G. Proceedings of the Fifth International Workshop on Advances in Electrocorticography. (2014) Epilepsy Behav. 41:183-92. DOI: 10.1016/j.yebeh.2014.09.015

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Calhoun, A.J., Chalasani, S.H., Sharpee, T.O. Maximally informative foraging by Caenorhabditis elegans. (2014) Elife. 3. DOI: 10.7554/eLife.04220

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Pankevich, D.E., Altevogt, B.M., Dunlop, J., Gage, F.H., Hyman, S.E. Improving and accelerating drug development for nervous system disorders. (2014) Neurona. 84(3):546-53. DOI: 10.1016/j.neuron.2014.10.007

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Regner, B.M., Tartakovsky, D.M., Sejnowski, T.J. Identifying transport behavior of single-molecule trajectories. (2014) Biophysical Journal. 107(10):2345-51. DOI: 10.1016/j.bpj.2014.10.005

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Carcagno, A.L., Di Bella, D.J., Goulding, M., Guillemot, F., Lanuza, G.M. Neurogenin3 restricts serotonergic neuron differentiation to the hindbrain. (2014) Diario de Neurociencia. 34(46):15223-33. DOI: 10.1523/JNEUROSCI.3403-14.2014

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Sejnowski, T.J., Churchland, P.S., Movshon, J.A. Putting big data to good use in neuroscience. (2014) Nature Neuroscience. 17(11):1440-1. DOI: 10.1038/nn.3839

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Kim, S.H., Steele, J.W., Lee, S.W., Clemenson, G.D., Carter, T.A., Treuner, K., Gadient, R., Wedel, P., Glabe, C., Barlow, C., Ehrlich, M.E., Gage, F.H., Gandy, S. Proneurogenic Group II mGluR antagonist improves learning and reduces anxiety in Alzheimer Aβ oligomer mouse. (2014) Psiquiatría Molecular. 19(11):1235-42. DOI: 10.1038/mp.2014.87

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Hook, V., Brennand, K.J., Kim, Y., Toneff, T., Funkelstein, L., Lee, K.C., Ziegler, M., Gage, F.H. Human iPSC neurons display activity-dependent neurotransmitter secretion: aberrant catecholamine levels in schizophrenia neurons. (2014) Stem Cell Reports. 3(4):531-8. DOI: 10.1016/j.stemcr.2014.08.001

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Aimone, J.B., Li, Y., Lee, S.W., Clemenson, G.D., Deng, W., Gage, F.H. Regulation and function of adult neurogenesis: from genes to cognition. (2014) Physiological Reviews. 94(4):991-1026. DOI: 10.1152/physrev.00004.2014

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Jessberger, S., Gage, F.H. Adult neurogenesis: bridging the gap between mice and humans. (2014) Trends in Cell Biology. 24(10):558-63. DOI: 10.1016/j.tcb.2014.07.003

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Fields, R.D., Araque, A., Johansen-Berg, H., Lim, S.S., Lynch, G., Nave, K.A., Nedergaard, M., Perez, R., Sejnowski, T., Wake, H. Glial biology in learning and cognition. (2014) The Neuroscientist. 20(5):426-431. DOI: 10.1177/1073858413504465

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Mak-McCully, R.A., Deiss, S.R., Rosen, B.Q., Jung, K.Y., Sejnowski, T.J., Bastuji, H., Rey, M., Cash, S.S., Bazhenov, M., Halgren, E. Synchronization of isolated downstates (K-complexes) may be caused by cortically-induced disruption of thalamic spindling. (2014) PLOS Biología Computacional. 10(9):e1003855. DOI: 10.1371/journal.pcbi.1003855

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Sleep spindles and K-complexes (KCs) define stage 2 NREM sleep (N2) in humans. We recently showed that KCs are isolated downstates characterized by widespread cortical silence. We demonstrate here that KCs can be quasi-synchronous across scalp EEG and across much of the cortex using electrocorticography (ECOG) and localized transcortical recordings (bipolar SEEG). We examine the mechanism of synchronous KC production by creating the first conductance based thalamocortical network model of N2 sleep to generate both spontaneous spindles and KCs. Spontaneous KCs are only observed when the model includes diffuse projections from restricted prefrontal areas to the thalamic reticular nucleus (RE), consistent with recent anatomical findings in rhesus monkeys. Modeled KCs begin with a spontaneous focal depolarization of the prefrontal neurons, followed by depolarization of the RE. Surprisingly, the RE depolarization leads to decreased firing due to disrupted spindling, which in turn is due to depolarization-induced inactivation of the low-threshold Ca2+ current (IT). Further, although the RE inhibits thalamocortical (TC) neurons, decreased RE firing causes decreased TC cell firing, again because of disrupted spindling. The resulting abrupt removal of excitatory input to cortical pyramidal neurons then leads to the downstate. Empirically, KCs may also be evoked by sensory stimuli while maintaining sleep. We reproduce this phenomenon in the model by depolarization of either the RE or the widely-projecting prefrontal neurons. Again, disruption of thalamic spindling plays a key role. Higher levels of RE stimulation also cause downstates, but by directly inhibiting the TC neurons. SEEG recordings from the thalamus and cortex in a single patient demonstrated the model prediction that thalamic spindling significantly decreases before KC onset. In conclusion, we show empirically that KCs can be widespread quasi-synchronous cortical downstates, and demonstrate with the first model of stage 2 NREM sleep a possible mechanism whereby this widespread synchrony may arise.

Stefan, M.I., Bartol, T.M., Sejnowski, T.J., Kennedy, M.B. Multi-state modeling of biomolecules. (2014) PLOS Biología Computacional. 10(9):e1003844. DOI: 10.1371/journal.pcbi.1003844

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Sharpee, T.O. Toward functional classification of neuronal types. (2014) Neurona. 83(6):1329-34. DOI: 10.1016/j.neuron.2014.08.040

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Garrett, M.E., Nauhaus, I., Marshel, J.H., Callaway, E.M. Topography and areal organization of mouse visual cortex. (2014) Diario de Neurociencia. 34(37):12587-12600. DOI: 10.1523/JNEUROSCI.1124-14.2014

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Winner, B., Marchetto, M.C., Winkler, J., Gage, F.H. Human-induced pluripotent stem cells pave the road for a better understanding of motor neuron disease. (2014) Genética Molecular Humana. 23(R1):R27-34. DOI: 10.1093/hmg/ddu205

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Gage, F., Nair, P. QnAs with Fred Gage. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(36):12958-9. DOI: 10.1073/pnas.1412625111

Moldakarimov, S., Bazhenov, M., Sejnowski, T.J. Top-down inputs enhance orientation selectivity in neurons of the primary visual cortex during perceptual learning. (2014) PLOS Biología Computacional. 10(8):e1003770. DOI: 10.1371/journal.pcbi.1003770

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Lee, H.S., Ghetti, A., Pinto-Duarte, A., Wang, X., Dziewczapolski, G., Galimi, F., Huitron-Resendiz, S., Piña-Crespo, J.C., Roberts, A.J., Verma, I.M., Sejnowski, T.J., Heinemann, S.F. Astrocytes contribute to gamma oscillations and recognition memory. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(32):E3343-52. DOI: 10.1073/pnas.1410893111

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Erwin, J.A., Marchetto, M.C., Gage, F.H. Mobile DNA elements in the generation of diversity and complexity in the brain. (2014) Nature Reviews Neuroscience. 15(8):497-506. DOI: 10.1038/nrn3730

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Gage, F., Pariente, N. On jumping genes and other things: Interview with Fred Gage, Adler Professor at the Salk Institute. (2014) EMBO Reports. 15(8):822-6. DOI: 10.15252/embr.201439088

Zhao, C., Jou, J., Wolff, L.J., Sun, H., Gage, F.H. Spine morphogenesis in newborn granule cells is differentially regulated in the outer and middle molecular layers. (2014) Journal of Comparative Neurology. 522(12):2756-66. DOI: 10.1002/cne.23581

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Weyhenmeyer, J., Hernandez, M.E., Lainscsek, C., Sejnowski, T.J., Poizner, H. Muscle artifacts in single trial EEG data distinguish patients with Parkinson's disease from healthy individuals. (2014) Conf Proc IEEE Eng Med Biol Soc. 2014:3292-3295. DOI: 10.1109/EMBC.2014.6944326

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Broccard, F.D., Mullen, T., Chi, Y.M., Peterson, D., Iversen, J.R., Arnold, M., Kreutz-Delgado, K., Jung, T.P., Makeig, S., Poizner, H., Sejnowski, T., Cauwenberghs, G. Closed-loop brain-machine-body interfaces for noninvasive rehabilitation of movement disorders. (2014) Ann Biomed Eng. 42(8):1573-1593. DOI: 10.1007/s10439-014-1032-6

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Bekinschtein, P., Kent, B.A., Oomen, C.A., Clemenson, G.D., Gage, F.H., Saksida, L.M., Bussey, T.J. Brain-derived neurotrophic factor interacts with adult-born immature cells in the dentate gyrus during consolidation of overlapping memories. (2014) Hipocampo. 24(8):905-11. DOI: 10.1002/hipo.22304

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Hatori, M., Gill, S., Mure, L.S., Goulding, M., O'Leary, D.D., Panda, S. Lhx1 maintains synchrony among circadian oscillator neurons of the SCN. (2014) Elife. 3:e03357. DOI: 10.7554/eLife.03357

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Ni, Y., Nawabi, H., Liu, X., Yang, L., Miyamichi, K., Tedeschi, A., Xu, B., Wall, N.R., Callaway, E.M., He, Z. Characterization of long descending premotor propriospinal neurons in the spinal cord. (2014) Diario de Neurociencia. 34(28):9404-9417. DOI: 10.1523/JNEUROSCI.1771-14.2014

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Stein, J.L., de la Torre-Ubieta, L., Tian, Y., Parikshak, N.N., Hernández, I.A., Marchetto, M.C., Baker, D.K., Lu, D., Hinman, C.R., Lowe, J.K., Wexler, E.M., Muotri, A.R., Gage, F.H., Kosik, K.S., Geschwind, D.H. A quantitative framework to evaluate modeling of cortical development by neural stem cells. (2014) Neurona. 83(1):69-86. DOI: 10.1016/j.neuron.2014.05.035

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Saremi, S., Sejnowski, T.J. On criticality in high-dimensional data. (2014) Computación neuronal. 26(7):1329-1339. DOI: 10.1162/NECO_a_00607

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Yu, D.X., Marchetto, M.C., Gage, F.H. How to make a hippocampal dentate gyrus granule neuron. (2014) Development. 141(12):2366-75. DOI: 10.1242/dev.096776

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Solstad, T., Yousif, H.N., Sejnowski, T.J. Place cell rate remapping by CA3 recurrent collaterals. (2014) PLOS Biología Computacional. 10(6):e1003648. DOI: 10.1371/journal.pcbi.1003648

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Episodic-like memory is thought to be supported by attractor dynamics in the hippocampus. A possible neural substrate for this memory mechanism is rate remapping, in which the spatial map of place cells encodes contextual information through firing rate variability. To test whether memories are stored as multimodal attractors in populations of place cells, recent experiments morphed one familiar context into another while observing the responses of CA3 cell ensembles. Average population activity in CA3 was reported to transition gradually rather than abruptly from one familiar context to the next, suggesting a lack of attractive forces associated with the two stored representations. On the other hand, individual CA3 cells showed a mix of gradual and abrupt transitions at different points along the morph sequence, and some displayed hysteresis which is a signature of attractor dynamics. To understand whether these seemingly conflicting results are commensurate with attractor network theory, we developed a neural network model of the CA3 with attractors for both position and discrete contexts. We found that for memories stored in overlapping neural ensembles within a single spatial map, position-dependent context attractors made transitions at different points along the morph sequence. Smooth transition curves arose from averaging across the population, while a heterogeneous set of responses was observed on the single unit level. In contrast, orthogonal memories led to abrupt and coherent transitions on both population and single unit levels as experimentally observed when remapping between two independent spatial maps. Strong recurrent feedback entailed a hysteretic effect on the network which diminished with the amount of overlap in the stored memories. These results suggest that context-dependent memory can be supported by overlapping local attractors within a spatial map of CA3 place cells. Similar mechanisms for context-dependent memory may also be found in other regions of the cerebral cortex.

Sharpee, T.O. Function determines structure in complex neural networks. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(23):8327-8. DOI: 10.1073/pnas.1407198111

Goulding, M., Bourane, S., Garcia-Campmany, L., Dalet, A., Koch, S. Inhibition downunder: an update from the spinal cord. (2014) Current Opinion in Neurobiology. 26:161-6. DOI: 10.1016/j.conb.2014.03.006

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Lacar, B., Parylak, S.L., Vadodaria, K.C., Sarkar, A., Gage, F.H. Increasing the resolution of the adult neurogenesis picture. (2014) F1000 Prime Reports. 6:8. DOI: 10.12703/P6-8

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Hrvoj-Mihic, B., Marchetto, M.C., Gage, F.H., Semendeferi, K., Muotri, A.R. Novel tools, classic techniques: evolutionary studies using primate pluripotent stem cells. (2014) Psiquiatría Biológica. 75(12):929-35. DOI: 10.1016/j.biopsych.2013.08.007

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Sejnowski, T.J. Summary: Cognition in 2014. (2014) Cold Spring Harbor Symposia on Quantitative Biology. 79:237-41. DOI: 10.1101/sqb.2014.79.024885

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Sejnowski, T.J., Poizner, H., Lynch, G., Gepshtein, S., Greenspan, R.J. Prospective Optimization. (2014) Proceedings of the IEEE. Institute of Electrical and Electronics Engineers. 102(5). DOI: 10.1109/JPROC.2014.2314297

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Knowland, D., Arac, A., Sekiguchi, K.J., Hsu, M., Lutz, S.E., Perrino, J., Steinberg, G.K., Barres, B.A., Nimmerjahn, A., Agalliu, D. Stepwise recruitment of transcellular and paracellular pathways underlies blood-brain barrier breakdown in stroke. (2014) Neurona. 82(3):603-17. DOI: 10.1016/j.neuron.2014.03.003

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Jadi, M.P., Sejnowski, T.J. Cortical oscillations arise from contextual interactions that regulate sparse coding. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(18):6780-5. DOI: 10.1073/pnas.1405300111

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Hashimoto-Torii, K., Torii, M., Fujimoto, M., Nakai, A., El Fatimy, R., Mezger, V., Ju, M.J., Ishii, S., Chao, S.H., Brennand, K.J., Gage, F.H., Rakic, P. Roles of heat shock factor 1 in neuronal response to fetal environmental risks and its relevance to brain disorders. (2014) Neurona. 82(3):560-72. DOI: 10.1016/j.neuron.2014.03.002

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Cetin, A., Callaway, E.M. Optical control of retrogradely infected neurons using drug-regulated "TLoop" lentiviral vectors. (2014) Journal of Neurophysiology. 111(10):2150-2159. DOI: 10.1152/jn.00495.2013

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Wang, L., Mongera, A., Bonanomi, D., Cyganek, L., Pfaff, S.L., Nüsslein-Volhard, C., Marquardt, T. A conserved axon type hierarchy governing peripheral nerve assembly. (2014) Development. 141(9):1875-83. DOI: 10.1242/dev.106211

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McDonnell, M.D., Boahen, K., Ijspeert, A., Sejnowski, T.J. Engineering Intelligent Electronic Systems Based on Computational Neuroscience. (2014) Proceedings of the IEEE. Institute of Electrical and Electronics Engineers. 102(5):646-651. DOI: 10.1109/JPROC.2014.2314776

Rockenstein, E., Nuber, S., Overk, C.R., Ubhi, K., Mante, M., Patrick, C., Adame, A., Trejo-Morales, M., Gerez, J., Picotti, P., Jensen, P.H., Campioni, S., Riek, R., Winkler, J., Gage, F.H., Winner, B., Masliah, E. Accumulation of oligomer-prone α-synuclein exacerbates synaptic and neuronal degeneration in vivo. (2014) Brain. 137(Pt 5):1496-513. DOI: 10.1093/brain/awu057

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In Parkinson's disease and dementia with Lewy bodies, α-synuclein aggregates to form oligomers and fibrils; however, the precise nature of the toxic α-synuclein species remains unclear. A number of synthetic α-synuclein mutations were recently created (E57K and E35K) that produce species of α-synuclein that preferentially form oligomers and increase α-synuclein-mediated toxicity. We have shown that acute lentiviral expression of α-synuclein E57K leads to the degeneration of dopaminergic neurons; however, the effects of chronic expression of oligomer-prone α-synuclein in synapses throughout the brain have not been investigated. Such a study could provide insight into the possible mechanism(s) through which accumulation of α-synuclein oligomers in the synapse leads to neurodegeneration. For this purpose, we compared the patterns of neurodegeneration and synaptic damage between a newly generated mThy-1 α-synuclein E57K transgenic mouse model that is prone to forming oligomers and the mThy-1 α-synuclein wild-type mouse model (Line 61), which accumulates various forms of α-synuclein. Three lines of α-synuclein E57K (Lines 9, 16 and 54) were generated and compared with the wild-type. The α-synuclein E57K Lines 9 and 16 were higher expressings of α-synuclein, similar to α-synuclein wild-type Line 61, and Line 54 was a low expressing of α-synuclein compared to Line 61. By immunoblot analysis, the higher-expressing α-synuclein E57K transgenic mice showed abundant oligomeric, but not fibrillar, α-synuclein whereas lower-expressing mice accumulated monomeric α-synuclein. Monomers, oligomers, and fibrils were present in α-synuclein wild-type Line 61. Immunohistochemical and ultrastructural analyses demonstrated that α-synuclein accumulated in the synapses but not in the neuronal cells bodies, which was different from the α-synuclein wild-type Line 61, which accumulates α-synuclein in the soma. Compared to non-transgenic and lower-expressing mice, the higher-expressing α-synuclein E57K mice displayed synaptic and dendritic loss, reduced levels of synapsin 1 and synaptic vesicles, and behavioural deficits. Similar alterations, but to a lesser extent, were seen in the α-synuclein wild-type mice. Moreover, although the oligomer-prone α-synuclein mice displayed neurodegeneration in the frontal cortex and hippocampus, the α-synuclein wild-type only displayed neuronal loss in the hippocampus. These results support the hypothesis that accumulating oligomeric α-synuclein may mediate early synaptic pathology in Parkinson's disease and dementia with Lewy bodies by disrupting synaptic vesicles. This oligomer-prone model might be useful for evaluating therapies directed at oligomer reduction.

Havlicek, S., Kohl, Z., Mishra, H.K., Prots, I., Eberhardt, E., Denguir, N., Wend, H., Plötz, S., Boyer, L., Marchetto, M.C., Aigner, S., Sticht, H., Groemer, T.W., Hehr, U., Lampert, A., Schlötzer-Schrehardt, U., Winkler, J., Gage, F.H., Winner, B. Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons. (2014) Genética Molecular Humana. 23(10):2527-41. DOI: 10.1093/hmg/ddt644

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Jadi, M.P., Sejnowski, T.J. Regulating Cortical Oscillations in an Inhibition-Stabilized Network. (2014) Proceedings of the IEEE. Institute of Electrical and Electronics Engineers. 102(5). DOI: 10.1109/JPROC.2014.2313113

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O'Donnell, C., Sejnowski, T.J. Selective memory generalization by spatial patterning of protein synthesis. (2014) Neurona. 82(2):398-412. DOI: 10.1016/j.neuron.2014.02.028

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Sharpee, T.O., Calhoun, A.J., Chalasani, S.H. Information theory of adaptation in neurons, behavior, and mood. (2014) Current Opinion in Neurobiology. 25:47-53. DOI: 10.1016/j.conb.2013.11.007

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Morris, R., Sancho-Martinez, I., Sharpee, T.O., Izpisua Belmonte, J.C. Mathematical approaches to modeling development and reprogramming. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(14):5076-82. DOI: 10.1073/pnas.1317150111

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Firth, A.L., Dargitz, C.T., Qualls, S.J., Menon, T., Wright, R., Singer, O., Gage, F.H., Khanna, A., Verma, I.M. Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(17):E1723-30. DOI: 10.1073/pnas.1403470111

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Zhang, J., Lanuza, G.M., Britz, O., Wang, Z., Siembab, V.C., Zhang, Y., Velasquez, T., Alvarez, F.J., Frank, E., Goulding, M. V1 and v2b interneurons secure the alternating flexor-extensor motor activity mice require for limbed locomotion. (2014) Neurona. 82(1):138-50. DOI: 10.1016/j.neuron.2014.02.013

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Sun, Y., Nguyen, A.Q., Nguyen, J.P., Le, L., Saur, D., Choi, J., Callaway, E.M., Xu, X. Cell-type-specific circuit connectivity of hippocampal CA1 revealed through Cre-dependent rabies tracing. (2014) Cell Reports. 7(1):269-280. DOI: 10.1016/j.celrep.2014.02.030

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Pao, G.M., Zhu, Q., Perez-Garcia, C.G., Chou, S.J., Suh, H., Gage, F.H., O'Leary, D.D., Verma, I.M. Role of BRCA1 in brain development. (2014) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 111(13):E1240-8. DOI: 10.1073/pnas.1400783111

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Levine, A.J., Hinckley, C.A., Hilde, K.L., Driscoll, S.P., Poon, T.H., Montgomery, J.M., Pfaff, S.L. Identification of a cellular node for motor control pathways. (2014) Nature Neuroscience. 17(4):586-93. DOI: 10.1038/nn.3675

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Crotti, A., Benner, C., Kerman, B.E., Gosselin, D., Lagier-Tourenne, C., Zuccato, C., Cattaneo, E., Gage, F.H., Cleveland, D.W., Glass, C.K. Mutant Huntingtin promotes autonomous microglia activation via myeloid lineage-determining factors. (2014) Nature Neuroscience. 17(4):513-21. DOI: 10.1038/nn.3668

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Edwards, J., Daniel, E., Kinney, J., Bartol, T., Sejnowski, T., Johnston, D., Harris, K., Bajaj, C. VolRoverN: enhancing surface and volumetric reconstruction for realistic dynamical simulation of cellular and subcellular function. (2014) Neuroinformatics. 12(2):277-289. DOI: 10.1007/s12021-013-9205-2

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Yu, D.X., Di Giorgio, F.P., Yao, J., Marchetto, M.C., Brennand, K., Wright, R., Mei, A., McHenry, L., Lisuk, D., Grasmick, J.M., Silberman, P., Silberman, G., Jappelli, R., Gage, F.H. Modeling hippocampal neurogenesis using human pluripotent stem cells. (2014) Stem Cell Reports. 2(3):295-310. DOI: 10.1016/j.stemcr.2014.01.009

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Cruz-Martín, A., El-Danaf, R.N., Osakada, F., Sriram, B., Dhande, O.S., Nguyen, P.L., Callaway, E.M., Ghosh, A., Huberman, A.D. A dedicated circuit links direction-selective retinal ganglion cells to the primary visual cortex. (2014) Naturaleza. 507(7492):358-361. DOI: 10.1038/nature12989

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Wright, R., Réthelyi, J.M., Gage, F.H. Enhancing induced pluripotent stem cell models of schizophrenia. (2014) JAMA Psychiatry. 71(3):334-5. DOI: 10.1001/jamapsychiatry.2013.4239

Yang, L., Leung, H., Peterson, D.A., Sejnowski, T.J., Poizner, H. Toward a semi-self-paced EEG brain computer interface: decoding initiation state from non-initiation state in dedicated time slots. (2014) PLOS One. 9(2):e88915. DOI: 10.1371/journal.pone.0088915

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Brain computer interfaces (BCIs) offer a broad class of neurologically impaired individuals an alternative means to interact with the environment. Many BCIs are "synchronous" systems, in which the system sets the timing of the interaction and tries to infer what control command the subject is issuing at each prompting. In contrast, in "asynchronous" BCIs subjects pace the interaction and the system must determine when the subject's control command occurs. In this paper we propose a new idea for BCI which draws upon the strengths of both approaches. The subjects are externally paced and the BCI is able to determine when control commands are issued by decoding the subject's intention for initiating control in dedicated time slots. A single task with randomly interleaved trials was designed to test whether it can be used as stimulus for inducing initiation and non-initiation states when the sensory and motor requirements for the two types of trials are very nearly identical. Further, the essential problem on the discrimination between initiation state and non-initiation state was studied. We tested the ability of EEG spectral power to distinguish between these two states. Among the four standard EEG frequency bands, beta band power recorded over parietal-occipital cortices provided the best performance, achieving an average accuracy of 86% for the correct classification of initiation and non-initiation states. Moreover, delta band power recorded over parietal and motor areas yielded a good performance and thus could also be used as an alternative feature to discriminate these two mental states. The results demonstrate the viability of our proposed idea for a BCI design based on conventional EEG features. Our proposal offers the potential to mitigate the signal detection challenges of fully asynchronous BCIs, while providing greater flexibility to the subject than traditional synchronous BCIs.

Vadodaria, K.C., Gage, F.H. SnapShot: adult hippocampal neurogenesis. (2014) Móvil. 156(5):1114-1114.e1. DOI: 10.1016/j.cell.2014.02.029

Rangel, L.M., Alexander, A.S., Aimone, J.B., Wiles, J., Gage, F.H., Chiba, A.A., Quinn, L.K. Temporally selective contextual encoding in the dentate gyrus of the hippocampus. (2014) Nature Communications. 5:3181. DOI: 10.1038/ncomms4181

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Parylak, S.L., Deng, W., Gage, F.H. Mother's milk programs offspring's cognition. (2014) Nature Neuroscience. 17(1):8-9. DOI: 10.1038/nn.3611

Marchetto, M.C., Gage, F.H. Your brain under the microscope: the promise of stem cells. (2014) Cerebrum. 2014:1.

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Lainscsek, C., Weyhenmeyer, J., Hernandez, M.E., Poizner, H., Sejnowski, T.J. Non-linear dynamical classification of short time series of the rössler system in high noise regimes. (2013) Frontiers in Neurology. 4:182. DOI: 10.3389/fneur.2013.00182

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Time series analysis with delay differential equations (DDEs) reveals non-linear properties of the underlying dynamical system and can serve as a non-linear time-domain classification tool. Here global DDE models were used to analyze short segments of simulated time series from a known dynamical system, the Rössler system, in high noise regimes. In a companion paper, we apply the DDE model developed here to classify short segments of encephalographic (EEG) data recorded from patients with Parkinson's disease and healthy subjects. Nine simulated subjects in each of two distinct classes were generated by varying the bifurcation parameter b and keeping the other two parameters (a and c) of the Rössler system fixed. All choices of b were in the chaotic parameter range. We diluted the simulated data using white noise ranging from 10 to -30 dB signal-to-noise ratios (SNR). Structure selection was supervised by selecting the number of terms, delays, and order of non-linearity of the model DDE model that best linearly separated the two classes of data. The distances d from the linear dividing hyperplane was then used to assess the classification performance by computing the area A' under the ROC curve. The selected model was tested on untrained data using repeated random sub-sampling validation. DDEs were able to accurately distinguish the two dynamical conditions, and moreover, to quantify the changes in the dynamics. There was a significant correlation between the dynamical bifurcation parameter b of the simulated data and the classification parameter d from our analysis. This correlation still held for new simulated subjects with new dynamical parameters selected from each of the two dynamical regimes. Furthermore, the correlation was robust to added noise, being significant even when the noise was greater than the signal. We conclude that DDE models may be used as a generalizable and reliable classification tool for even small segments of noisy data.

Lainscsek, C., Hernandez, M.E., Weyhenmeyer, J., Sejnowski, T.J., Poizner, H. Non-linear dynamical analysis of EEG time series distinguishes patients with Parkinson's disease from healthy individuals. (2013) Frontiers in Neurology. 4:200. DOI: 10.3389/fneur.2013.00200

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The pathophysiology of Parkinson's disease (PD) is known to involve altered patterns of neuronal firing and synchronization in cortical-basal ganglia circuits. One window into the nature of the aberrant temporal dynamics in the cerebral cortex of PD patients can come from analysis of the patients electroencephalography (EEG). Rather than using spectral-based methods, we used data models based on delay differential equations (DDE) as non-linear time-domain classification tools to analyze EEG recordings from PD patients on and off dopaminergic therapy and healthy individuals. Two sets of 50 1-s segments of 64-channel EEG activity were recorded from nine PD patients on and off medication and nine age-matched controls. The 64 EEG channels were grouped into 10 clusters covering frontal, central, parietal, and occipital brain regions for analysis. DDE models were fitted to individual trials, and model coefficients and error were used as features for classification. The best models were selected using repeated random sub-sampling validation and classification performance was measured using the area under the ROC curve A'. In a companion paper, we show that DDEs can uncover hidden dynamical structure from short segments of simulated time series of known dynamical systems in high noise regimes. Using the same method for finding the best models, we found here that even short segments of EEG data in PD patients and controls contained dynamical structure, and moreover, that PD patients exhibited a greater dynamic range than controls. DDE model output on the means from one set of 50 trials provided nearly complete separation of PD patients off medication from controls: across brain regions, the area under the receiver-operating characteristic curves, A', varied from 0.95 to 1.0. For distinguishing PD patients on vs. off medication, classification performance A' ranged from 0.86 to 1.0 across brain regions. Moreover, the generalizability of the model to the second set of 50 trials was excellent, with A' ranging from 0.81 to 0.94 across brain regions for controls vs. PD off medication, and from 0.62 to 0.82 for PD on medication vs. off. Finally, model features significantly predicted individual patients' motor severity, as assessed with standard clinical rating scales.

Pfaff, S., Shaham, S. Development of neurons and glia. (2013) Current Opinion in Neurobiology. 23(6):901-2.

Grindberg, R.V., Yee-Greenbaum, J.L., McConnell, M.J., Novotny, M., O'Shaughnessy, A.L., Lambert, G.M., Araúzo-Bravo, M.J., Lee, J., Fishman, M., Robbins, G.E., Lin, X., Venepally, P., Badger, J.H., Galbraith, D.W., Gage, F.H., Lasken, R.S. RNA-sequencing from single nuclei. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(49):19802-7. DOI: 10.1073/pnas.1319700110

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Borowska, J., Jones, C.T., Zhang, H., Blacklaws, J., Goulding, M., Zhang, Y. Functional subpopulations of V3 interneurons in the mature mouse spinal cord. (2013) Diario de Neurociencia. 33(47):18553-65. DOI: 10.1523/JNEUROSCI.2005-13.2013

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Del Barrio, M.G., Bourane, S., Grossmann, K., Schüle, R., Britsch, S., O'Leary, D.D., Goulding, M. A transcription factor code defines nine sensory interneuron subtypes in the mechanosensory area of the spinal cord. (2013) PLOS One. 8(11):e77928. DOI: 10.1371/journal.pone.0077928

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Reilly, M.T., Faulkner, G.J., Dubnau, J., Ponomarev, I., Gage, F.H. The role of transposable elements in health and diseases of the central nervous system. (2013) Diario de Neurociencia. 33(45):17577-86. DOI: 10.1523/JNEUROSCI.3369-13.2013

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McConnell, M.J., Lindberg, M.R., Brennand, K.J., Piper, J.C., Voet, T., Cowing-Zitron, C., Shumilina, S., Lasken, R.S., Vermeesch, J.R., Hall, I.M., Gage, F.H. Mosaic copy number variation in human neurons. (2013) Ciencia. 342(6158):632-7. DOI: 10.1126/science.1243472

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Marchetto, M.C.N., Narvaiza, I., Denli, A.M., Benner, C., Lazzarini, T.A., Nathanson, J.L., Paquola, A.C.M., Desai, K.N., Herai, R.H., Weitzman, M.D., Yeo, G.W., Muotri, A.R., Gage, F.H. Differential L1 regulation in pluripotent stem cells of humans and apes. (2013) Naturaleza. 503(7477):525-529. DOI: 10.1038/nature12686

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Identifying cellular and molecular differences between human and non-human primates (NHPs) is essential to the basic understanding of the evolution and diversity of our own species. Until now, preserved tissues have been the main source for most comparative studies between humans, chimpanzees (Pan troglodytes) and bonobos (Pan paniscus). However, these tissue samples do not fairly represent the distinctive traits of live cell behaviour and are not amenable to genetic manipulation. We propose that induced pluripotent stem (iPS) cells could be a unique biological resource to determine relevant phenotypical differences between human and NHPs, and that those differences could have potential adaptation and speciation value. Here we describe the generation and initial characterization of iPS cells from chimpanzees and bonobos as new tools to explore factors that may have contributed to great ape evolution. Comparative gene expression analysis of human and NHP iPS cells revealed differences in the regulation of long interspersed element-1 (L1, also known as LINE-1) transposons. A force of change in mammalian evolution, L1 elements are retrotransposons that have remained active during primate evolution. Decreased levels of L1-restricting factors APOBEC3B (also known as A3B) and PIWIL2 (ref. 7) in NHP iPS cells correlated with increased L1 mobility and endogenous L1 messenger RNA levels. Moreover, results from the manipulation of A3B and PIWIL2 levels in iPS cells supported a causal inverse relationship between levels of these proteins and L1 retrotransposition. Finally, we found increased copy numbers of species-specific L1 elements in the genome of chimpanzees compared to humans, supporting the idea that increased L1 mobility in NHPs is not limited to iPS cells in culture and may have also occurred in the germ line or embryonic cells developmentally upstream to germline specification during primate evolution. We propose that differences in L1 mobility may have differentially shaped the genomes of humans and NHPs and could have continuing adaptive significance.

Bekinschtein, P., Kent, B.A., Oomen, C.A., Clemenson, G.D., Gage, F.H., Saksida, L.M., Bussey, T.J. BDNF in the dentate gyrus is required for consolidation of "pattern-separated" memories. (2013) Cell Reports. 5(3):759-68. DOI: 10.1016/j.celrep.2013.09.027

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Miller, J.A., Nathanson, J., Franjic, D., Shim, S., Dalley, R.A., Shapouri, S., Smith, K.A., Sunkin, S.M., Bernard, A., Bennett, J.L., Lee, C.K., Hawrylycz, M.J., Jones, A.R., Amaral, D.G., Šestan, N., Gage, F.H., Lein, E.S. Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates. (2013) Development. 140(22):4633-44. DOI: 10.1242/dev.097212

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Gage, F.H., Temple, S. Neural stem cells: generating and regenerating the brain. (2013) Neurona. 80(3):588-601. DOI: 10.1016/j.neuron.2013.10.037

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Deshmukh, V.A., Tardif, V., Lyssiotis, C.A., Green, C.C., Kerman, B., Kim, H.J., Padmanabhan, K., Swoboda, J.G., Ahmad, I., Kondo, T., Gage, F.H., Theofilopoulos, A.N., Lawson, B.R., Schultz, P.G., Lairson, L.L. A regenerative approach to the treatment of multiple sclerosis. (2013) Naturaleza. 502(7471):327-332. DOI: 10.1038/nature12647

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Devor, A., Bandettini, P.A., Boas, D.A., Bower, J.M., Buxton, R.B., Cohen, L.B., Dale, A.M., Einevoll, G.T., Fox, P.T., Franceschini, M.A., Friston, K.J., Fujimoto, J.G., Geyer, M.A., Greenberg, J.H., Halgren, E., Hämäläinen, M.S., Helmchen, F., Hyman, B.T., Jasanoff, A., Jernigan, T.L., Judd, L.L., Kim, S.G., Kleinfeld, D., Kopell, N.J., Kutas, M., Kwong, K.K., Larkum, M.E., Lo, E.H., Magistretti, P.J., Mandeville, J.B., Masliah, E., Mitra, P.P., Mobley, W.C., Moskowitz, M.A., Nimmerjahn, A., Reynolds, J.H., Rosen, B.R., Salzberg, B.M., Schaffer, C.B., Silva, G.A., So, P.T., Spitzer, N.C., Tootell, R.B., Van Essen, D.C., Vanduffel, W., Vinogradov, S.A., Wald, L.L., Wang, L.V., Weber, B., Yodh, A.G. The challenge of connecting the dots in the B.R.A.I.N. (2013) Neurona. 80(2):270-4. DOI: 10.1016/j.neuron.2013.09.008

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Cortes, J.M., Desroches, M., Rodrigues, S., Veltz, R., Muñoz, M.A., Sejnowski, T.J. Short-term synaptic plasticity in the deterministic Tsodyks-Markram model leads to unpredictable network dynamics. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(41):16610-5. DOI: 10.1073/pnas.1316071110

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Herzog, C.D., Brown, L., Kruegel, B.R., Wilson, A., Tansey, M.G., Gage, F.H., Johnson, E.M., Bartus, R.T. Enhanced neurotrophic distribution, cell signaling and neuroprotection following substantia nigral versus striatal delivery of AAV2-NRTN (CERE-120). (2013) Neurobiol. Dis. 58:38-48. DOI: 10.1016/j.nbd.2013.04.011

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This paper reassesses the currently accepted viewpoint that targeting the terminal fields (i.e. striatum) of degenerating nigrostriatal dopamine neurons with neurotrophic factors in Parkinson's disease (PD) is sufficient for achieving an optimal neurotrophic response. Recent insight indicating that PD is an axonopathy characterized by axonal transport deficits prompted this effort. We tested whether a significantly greater neurotrophic response might be induced in SN neurons when the neurotrophic factor neurturin (NRTN) is also targeted to the substantia nigra (SN), compared to the more conventional, striatum-only target. While recognizing the importance of maintaining the integrity of nigrostriatal fibers and terminals (especially for achieving optimal function), we refocused attention to the fate of SN neurons. Under conditions of axonal degeneration and neuronal transport deficits, this component of the nigrostriatal system is most vulnerable to the lack of neurotrophic exposure following striatal-only delivery. Given the location of repair genes induced by neurotrophic factors, achieving adequate neurotrophic exposure to the SN neurons is essential for an optimal neurotrophic response, while the survival of these neurons is essential to the very survival of the fibers. Two separate studies were performed using the 6-OHDA model of nigrostriatal degeneration, in conjunction with delivery of the viral vector AAV2-NRTN (CERE-120) to continuously express NRTN to either striatum or nigra alone or combined striatal/nigral exposure, including conditions of ongoing axonopathy. These studies provide additional insight for reinterpreting past animal neurotrophic/6-OHDA studies conducted under conditions where axon transport deficiencies were generally not accounted for, which suggested that targeting the striatum was both necessary and sufficient. The current data demonstrate that delivering NRTN directly to the SN produces 1) expanded NRTN distribution within the terminal field and cell bodies of targeted nigrostriatal neurons, 2) enhanced intracellular neurotrophic factor signaling in the nigrostriatal neurons, and 3) produced greater numbers of surviving dopamine neurons against 6-OHDA-induced toxicity, particularly under the conditions of active axonopathy. Thus, these data provide empirical support that targeting the SN with neurotrophic factors (in addition to striatum) may help enhance the neurotrophic response in midbrain neurons, particularly under conditions of active neurodegeneration which occurs in PD patients.

Aljadeff, J., Segev, R., Berry, M.J., Sharpee, T.O. Spike triggered covariance in strongly correlated gaussian stimuli. (2013) PLOS Biología Computacional. 9(9):e1003206. DOI: 10.1371/journal.pcbi.1003206

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Ruiz, O., Lustig, B.R., Nassi, J.J., Cetin, A., Reynolds, J.H., Albright, T.D., Callaway, E.M., Stoner, G.R., Roe, A.W. Optogenetics through windows on the brain in the nonhuman primate. (2013) Journal of Neurophysiology. 110(6):1455-67. DOI: 10.1152/jn.00153.2013

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Hsu, C.C., White, N.M., Hayashi, M., Lin, E.C., Poon, T., Banerjee, I., Chen, J., Pfaff, S.L., Macagno, E.R., Dorrestein, P.C. Microscopy ambient ionization top-down mass spectrometry reveals developmental patterning. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(37):14855-60. DOI: 10.1073/pnas.1310618110

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Timofeev, I., Sejnowski, T.J., Bazhenov, M., Chauvette, S., Grand, L.B. Age dependency of trauma-induced neocortical epileptogenesis. (2013) Frontiers in Cellular Neuroscience. 7:154. DOI: 10.3389/fncel.2013.00154

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Tang, X., Zhou, L., Wagner, A.M., Marchetto, M.C., Muotri, A.R., Gage, F.H., Chen, G. Astroglial cells regulate the developmental timeline of human neurons differentiated from induced pluripotent stem cells. (2013) Stem Cell Research. 11(2):743-57. DOI: 10.1016/j.scr.2013.05.002

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Osakada, F., Callaway, E.M. Design and generation of recombinant rabies virus vectors. (2013) Nature Protocols. 8(8):1583-1601. DOI: 10.1038/nprot.2013.094

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Lister, R.*, Mukamel, E.A., Nery, J.R., Urich, M., Puddifoot, C.A., Johnson, N.D., Lucero, J., Huang, Y., Dwork, A.J., Schultz, M.D., Yu, M., Tonti-Filippini, J., Heyn, H., Hu, S., Wu, J.C., Rao, A., Esteller, M., He, C., Haghighi, F.G., Sejnowski, T.J., Behrens, M.M.*, Ecker, J.R.* Global epigenomic reconfiguration during mammalian brain development. (2013) Ciencia. 341(6146):1237905. DOI: 10.1126/science.1237905

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Francius, C., Harris, A., Rucchin, V., Hendricks, T.J., Stam, F.J., Barber, M., Kurek, D., Grosveld, F.G., Pierani, A., Goulding, M., Clotman, F. Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord. (2013) PLOS One. 8(8):e70325. DOI: 10.1371/journal.pone.0070325

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Sung, T.C., Chen, Z., Thuret, S., Vilar, M., Gage, F.H., Riek, R., Lee, K.F. P45 forms a complex with FADD and promotes neuronal cell survival following spinal cord injury. (2013) PLOS One. 8(7):e69286. DOI: 10.1371/journal.pone.0069286

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Sharpee, T.O. Computational identification of receptive fields. (2013) Revisión Anual de Neurociencia. 36:103-20. DOI: 10.1146/annurev-neuro-062012-170253

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Nienborg, H., Hasenstaub, A., Nauhaus, I., Taniguchi, H., Huang, Z.J., Callaway, E.M. Contrast dependence and differential contributions from somatostatin- and parvalbumin-expressing neurons to spatial integration in mouse V1. (2013) Diario de Neurociencia. 33(27):11145-11154. DOI: 10.1523/JNEUROSCI.5320-12.2013

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Wall, N.R., De La Parra, M., Callaway, E.M., Kreitzer, A.C. Differential innervation of direct- and indirect-pathway striatal projection neurons. (2013) Neurona. 79(2):347-360. DOI: 10.1016/j.neuron.2013.05.014

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Sharpee, T.O., Kouh, M., Reynolds, J.H. Trade-off between curvature tuning and position invariance in visual area V4. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(28):11618-23. DOI: 10.1073/pnas.1217479110

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Kaardal, J., Fitzgerald, J.D., Berry, M.J., Sharpee, T.O. Identifying functional bases for multidimensional neural computations. (2013) Computación neuronal. 25(7):1870-90. DOI: 10.1162/NECO_a_00465

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Current dimensionality-reduction methods can identify relevant subspaces for neural computations but do not favor one basis over the other within the relevant subspace. Finding the appropriate basis can simplify the description of the nonlinear computation with respect to the relevant variables, making it easier to elucidate the underlying neural computation and make hypotheses about the neural circuitry, giving rise to the observed responses. Part of the problem is that although some of the dimensionality reduction methods can identify many of the relevant dimensions, it is usually difficult to map out or interpret the nonlinear transformation with respect to more than a few relevant dimensions simultaneously without some simplifying assumptions. While recent approaches make it possible to create predictive models based on many relevant dimensions simultaneously, there still remains the need to relate such predictive models to the mechanistic descriptions of the operation of underlying neural circuitry. Here we demonstrate that transforming to a basis within the relevant subspace where the neural computation is best described by a given nonlinear function often makes it easier to interpret the computation and describe it with a small number of parameters. We refer to the corresponding basis as the functional basis, and illustrate the utility of such transformation in the context of logical OR and logical AND functions. We show that although dimensionality-reduction methods such as spike-triggered covariance are able to find a relevant subspace, they often produce dimensions that are difficult to interpret and do not correspond to a functional basis. The functional features can be found using a maximum likelihood approach. The results are illustrated using simulated neurons and recordings from retinal ganglion cells. The resulting features are uniquely defined and nonorthogonal, and they make it easier to relate computational and mechanistic models to each other.

Golomb, B.A., Brenner, S., Chalfie, M., Glashow, S.L., Glauber, R.J., Hubel, D.H., Maskin, E.S., Greengard, P., Gross, D.J., Roberts, R., Tonegawa, S., Wilczek, F.A., Brown, E.M., Sejnowski, T.J. Chocolate habits of Nobel prizewinners. (2013) Naturaleza. 499(7459):409. DOI: 10.1038/499409a

Lainscsek, C., Sejnowski, T.J. Electrocardiogram classification using delay differential equations. (2013) Chaos. 23(2):023132. DOI: 10.1063/1.4811544

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Nandy, A.S., Sharpee, T.O., Reynolds, J.H., Mitchell, J.F. The fine structure of shape tuning in area V4. (2013) Neurona. 78(6):1102-15. DOI: 10.1016/j.neuron.2013.04.016

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Yu, D.X., Marchetto, M.C., Gage, F.H. Therapeutic translation of iPSCs for treating neurological disease. (2013) Células Madre. 12(6):678-88. DOI: 10.1016/j.stem.2013.05.018

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Vilchez, D., Boyer, L., Lutz, M., Merkwirth, C., Morantte, I., Tse, C., Spencer, B., Page, L., Masliah, E., Berggren, W.T., Gage, F.H., Dillin, A. FOXO4 is necessary for neural differentiation of human embryonic stem cells. (2013) Célula en Envejecimiento. 12(3):518-22. DOI: 10.1111/acel.12067

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Ji, B., Wang, X., Pinto-Duarte, A., Kim, M., Caldwell, S., Young, J.W., Behrens, M.M., Sejnowski, T.J., Geyer, M.A., Zhou, X. Prolonged Ketamine Effects in Sp4 Hypomorphic Mice: Mimicking Phenotypes of Schizophrenia. (2013) PLOS One. 8(6):e66327. DOI: 10.1371/journal.pone.0066327

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Chukoskie, L., Snider, J., Mozer, M.C., Krauzlis, R.J., Sejnowski, T.J. Learning where to look for a hidden target. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110 Suppl :10438-10445. DOI: 10.1073/pnas.1301216110

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Egawa, N., Kitaoka, S., Tsukita, K., Naitoh, M., Takahashi, K., Yamamoto, T., Adachi, F., Kondo, T., Okita, K., Asaka, I., Aoi, T., Watanabe, A., Yamada, Y., Morizane, A., Takahashi, J., Ayaki, T., Ito, H., Yoshikawa, K., Yamawaki, S., Suzuki, S., Watanabe, D., Hioki, H., Kaneko, T., Makioka, K., Okamoto, K., Takuma, H., Tamaoka, A., Hasegawa, K., Nonaka, T., Hasegawa, M., Kawata, A., Yoshida, M., Nakahata, T., Takahashi, R., Marchetto, M.C., Gage, F.H., Yamanaka, S., Inoue, H. Response to comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells". (2013) Medicina Traslacional en Ciencia. 5(188):188lr2. DOI: 10.1126/scitranslmed.3005697

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Li, Y., Stam, F.J., Aimone, J.B., Goulding, M., Callaway, E.M., Gage, F.H. Molecular layer perforant path-associated cells contribute to feed-forward inhibition in the adult dentate gyrus. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(22):9106-11. DOI: 10.1073/pnas.1306912110

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Stiefel, K.M., Englitz, B., Sejnowski, T.J. Origin of intrinsic irregular firing in cortical interneurons. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(19):7886-7891. DOI: 10.1073/pnas.1305219110

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Gifford, W.D., Pfaff, S.L., Macfarlan, T.S. Transposable elements as genetic regulatory substrates in early development. (2013) Trends in Cell Biology. 23(5):218-26. DOI: 10.1016/j.tcb.2013.01.001

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Yin, L., Geng, Y., Osakada, F., Sharma, R., Cetin, A.H., Callaway, E.M., Williams, D.R., Merigan, W.H. Imaging light responses of retinal ganglion cells in the living mouse eye. (2013) Journal of Neurophysiology. 109(9):2415-2421. DOI: 10.1152/jn.01043.2012

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Gire, D.H., Restrepo, D., Sejnowski, T.J., Greer, C., De Carlos, J.A., Lopez-Mascaraque, L. Temporal processing in the olfactory system: can we see a smell? (2013) Neurona. 78(3):416-432. DOI: 10.1016/j.neuron.2013.04.033

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Rangel, L.M., Quinn, L.K., Chiba, A.A., Gage, F.H., Aimone, J.B. A hypothesis for temporal coding of young and mature granule cells. (2013) Fronteras en Neurociencia. 7:75. DOI: 10.3389/fnins.2013.00075

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Saremi, S., Sejnowski, T.J., Sharpee, T.O. Double-gabor filters are independent components of small translation-invariant image patches. (2013) Computación neuronal. 25(4):922-39. DOI: 10.1162/NECO_a_00418

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Wang, X., Pinto-Duarte, A., Sejnowski, T.J., Behrens, M.M. How Nox2-containing NADPH oxidase affects cortical circuits in the NMDA receptor antagonist model of schizophrenia. (2013) Antioxidantes y señalización redox. 18(12):1444-62. DOI: 10.1089/ars.2012.4907

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Jeanne, J.M., Sharpee, T.O., Gentner, T.Q. Associative learning enhances population coding by inverting interneuronal correlation patterns. (2013) Neurona. 78(2):352-63. DOI: 10.1016/j.neuron.2013.02.023

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Regner, B.M., Vučinić, D., Domnisoru, C., Bartol, T.M., Hetzer, M.W., Tartakovsky, D.M., Sejnowski, T.J. Anomalous diffusion of single particles in cytoplasm. (2013) Biophysical Journal. 104(8):1652-60. DOI: 10.1016/j.bpj.2013.01.049

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Wang, X., Zhao, Y., Zhang, X., Badie, H., Zhou, Y., Mu, Y., Loo, L.S., Cai, L., Thompson, R.C., Yang, B., Chen, Y., Johnson, P.F., Wu, C., Bu, G., Mobley, W.C., Zhang, D., Gage, F.H., Ranscht, B., Zhang, Y.W., Lipton, S.A., Hong, W., Xu, H. Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome. (2013) Medicina Natural. 19(4):473-80. DOI: 10.1038/nm.3117

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Deng, W., Mayford, M., Gage, F.H. Selection of distinct populations of dentate granule cells in response to inputs as a mechanism for pattern separation in mice. (2013) Elife. 2:e00312. DOI: 10.7554/eLife.00312

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Hinckley, C.A., Pfaff, S.L. Imaging spinal neuron ensembles active during locomotion with genetically encoded calcium indicators. (2013) Ann. N. Y. Acad. Sci. 1279:71-9. DOI: 10.1111/nyas.12092

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Rowe, H.M., Kapopoulou, A., Corsinotti, A., Fasching, L., Macfarlan, T.S., Tarabay, Y., Viville, S., Jakobsson, J., Pfaff, S.L., Trono, D. TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells. (2013) Investigación del genoma. 23(3):452-61. DOI: 10.1101/gr.147678.112

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Alivisatos, A.P., Andrews, A.M., Boyden, E.S., Chun, M., Church, G.M., Deisseroth, K., Donoghue, J.P., Fraser, S.E., Lippincott-Schwartz, J., Looger, L.L., Masmanidis, S., McEuen, P.L., Nurmikko, A.V., Park, H., Peterka, D.S., Reid, C., Roukes, M.L., Scherer, A., Schnitzer, M., Sejnowski, T.J., Shepard, K.L., Tsao, D., Turrigiano, G., Weiss, P.S., Xu, C., Yuste, R., Zhuang, X. Nanotools for neuroscience and brain activity mapping. (2013) ACS Nano. 7(3):1850-66. DOI: 10.1021/nn4012847

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Alivisatos, A.P., Chun, M., Church, G.M., Deisseroth, K., Donoghue, J.P., Greenspan, R.J., McEuen, P.L., Roukes, M.L., Sejnowski, T.J., Weiss, P.S., Yuste, R. Neuroscience. The brain activity map. (2013) Ciencia. 339(6125):1284-5. DOI: 10.1126/science.1236939

Liang, Y., Franks, T.M., Marchetto, M.C., Gage, F.H., Hetzer, M.W. Dynamic association of NUP98 with the human genome. (2013) PLOS Genetics. 9(2):e1003308. DOI: 10.1371/journal.pgen.1003308

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Saremi, S., Sejnowski, T.J. Hierarchical model of natural images and the origin of scale invariance. (2013) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 110(8):3071-3076. DOI: 10.1073/pnas.1222618110

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Tanaka, H., Sejnowski, T.J. Computing reaching dynamics in motor cortex with Cartesian spatial coordinates. (2013) Journal of Neurophysiology. 109(4):1182-1201. DOI: 10.1152/jn.00279.2012

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Kinney, J.P., Spacek, J., Bartol, T.M., Bajaj, C.L., Harris, K.M., Sejnowski, T.J. Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil. (2013) Journal of Comparative Neurology. 521(2):448-464. DOI: 10.1002/cne.23181

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Srinivasan, L., Asaad, W.F., Ginat, D.T., Gale, J.T., Dougherty, D.D., Williams, Z.M., Sejnowski, T.J., Eskandar, E.N. Action initiation in the human dorsal anterior cingulate cortex. (2013) PLOS One. 8(2):e55247. DOI: 10.1371/journal.pone.0055247

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Shimozaki, K., Clemenson, G.D., Gage, F.H. Paired related homeobox protein 1 is a regulator of stemness in adult neural stem/progenitor cells. (2013) Diario de Neurociencia. 33(9):4066-75. DOI: 10.1523/JNEUROSCI.4586-12.2013

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Volman, V., Bazhenov, M., Sejnowski, T.J. Divide and conquer: functional segregation of synaptic inputs by astrocytic microdomains could alleviate paroxysmal activity following brain trauma. (2013) PLOS Biología Computacional. 9(1):e1002856. DOI: 10.1371/journal.pcbi.1002856

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Kurian, L., Sancho-Martinez, I., Nivet, E., Aguirre, A., Moon, K., Pendaries, C., Volle-Challier, C., Bono, F., Herbert, J.M., Pulecio, J., Xia, Y., Li, M., Montserrat, N., Ruiz, S., Dubova, I., Rodriguez, C., Denli, A.M., Boscolo, F.S., Thiagarajan, R.D., Gage, F.H., Loring, J.F., Laurent, L.C., Izpisua Belmonte, J.C. Conversion of human fibroblasts to angioblast-like progenitor cells. (2013) Nature Methods. 10(1):77-83. DOI: 10.1038/nmeth.2255

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Nauhaus, I., Nielsen, K.J., Disney, A.A., Callaway, E.M. Orthogonal micro-organization of orientation and spatial frequency in primate primary visual cortex. (2012) Nature Neuroscience. 15(12):1683-1690. DOI: 10.1038/nn.3255

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Brennand, K.J., Simone, A., Tran, N., Gage, F.H. Modeling psychiatric disorders at the cellular and network levels. (2012) Psiquiatría Molecular. 17(12):1239-53. DOI: 10.1038/mp.2012.20

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Fava, M., Targum, S.D., Nierenberg, A.A., Bleicher, L.S., Carter, T.A., Wedel, P.C., Hen, R., Gage, F.H., Barlow, C. An exploratory study of combination buspirone and melatonin SR in major depressive disorder (MDD): a possible role for neurogenesis in drug discovery. (2012) J Psychiatr Res. 46(12):1553-63. DOI: 10.1016/j.jpsychires.2012.08.013

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Marshel, J.H., Kaye, A.P., Nauhaus, I., Callaway, E.M. Anterior-posterior direction opponency in the superficial mouse lateral geniculate nucleus. (2012) Neurona. 76(4):713-720. DOI: 10.1016/j.neuron.2012.09.021

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Goulding, M. Motor neurons that multitask. (2012) Neurona. 76(4):669-70. DOI: 10.1016/j.neuron.2012.11.011

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Winner, B., Regensburger, M., Schreglmann, S., Boyer, L., Prots, I., Rockenstein, E., Mante, M., Zhao, C., Winkler, J., Masliah, E., Gage, F.H. Role of α-synuclein in adult neurogenesis and neuronal maturation in the dentate gyrus. (2012) Diario de Neurociencia. 32(47):16906-16. DOI: 10.1523/JNEUROSCI.2723-12.2012

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Bellin, M., Marchetto, M.C., Gage, F.H., Mummery, C.L. Induced pluripotent stem cells: the new patient? (2012) Nature Reviews Molecular Cell Biology. 13(11):713-26. DOI: 10.1038/nrm3448

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Levine, A.J., Lewallen, K.A., Pfaff, S.L. Spatial organization of cortical and spinal neurons controlling motor behavior. (2012) Current Opinion in Neurobiology. 22(5):812-21. DOI: 10.1016/j.conb.2012.07.002

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Toups, J.V., Fellous, J.M., Thomas, P.J., Sejnowski, T.J., Tiesinga, P.H. Multiple spike time patterns occur at bifurcation points of membrane potential dynamics. (2012) PLOS Biología Computacional. 8(10):e1002615. DOI: 10.1371/journal.pcbi.1002615

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Catterall, W.A., Raman, I.M., Robinson, H.P., Sejnowski, T.J., Paulsen, O. The Hodgkin-Huxley heritage: from channels to circuits. (2012) Diario de Neurociencia. 32(41):14064-14073. DOI: 10.1523/JNEUROSCI.3403-12.2012

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Vilchez, D., Boyer, L., Morantte, I., Lutz, M., Merkwirth, C., Joyce, D., Spencer, B., Page, L., Masliah, E., Berggren, W.T., Gage, F.H., Dillin, A. Increased proteasome activity in human embryonic stem cells is regulated by PSMD11. (2012) Naturaleza. 489(7415):304-8. DOI: 10.1038/nature11468

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Eickenberg, M., Rowekamp, R.J., Kouh, M., Sharpee, T.O. Characterizing responses of translation-invariant neurons to natural stimuli: maximally informative invariant dimensions. (2012) Computación neuronal. 24(9):2384-421. DOI: 10.1162/NECO_a_00330

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The human visual system is capable of recognizing complex objects even when their appearances change drastically under various viewing conditions. Especially in the higher cortical areas, the sensory neurons reflect such functional capacity in their selectivity for complex visual features and invariance to certain object transformations, such as image translation. Due to the strong nonlinearities necessary to achieve both the selectivity and invariance, characterizing and predicting the response patterns of these neurons represents a formidable computational challenge. A related problem is that such neurons are poorly driven by randomized inputs, such as white noise, and respond strongly only to stimuli with complex high-order correlations, such as natural stimuli. Here we describe a novel two-step optimization technique that can characterize both the shape selectivity and the range and coarseness of position invariance from neural responses to natural stimuli. One step in the optimization is finding the template as the maximally informative dimension given the estimated spatial location where the response could have been triggered within each image. The estimates of the locations that triggered the response are updated in the next step. Under the assumption of a monotonic relationship between the firing rate and stimulus projections on the template at a given position, the most likely location is the one that has the largest projection on the estimate of the template. The algorithm shows quick convergence during optimization, and the estimation results are reliable even in the regime of small signal-to-noise ratios. When we apply the algorithm to responses of complex cells in the primary visual cortex (V1) to natural movies, we find that responses of the majority of cells were significantly better described by translation-invariant models based on one template compared with position-specific models with several relevant features.

Nadkarni, S., Bartol, T.M., Stevens, C.F., Sejnowski, T.J., Levine, H. Short-term plasticity constrains spatial organization of a hippocampal presynaptic terminal. (2012) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 109(36):14657-14662. DOI: 10.1073/pnas.1211971109

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Volman, V., Bazhenov, M., Sejnowski, T.J. Computational models of neuron-astrocyte interaction in epilepsy. (2012) Frontiers in Computational Neuroscience. 6:58. DOI: 10.3389/fncom.2012.00058

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Boyer, L.F., Campbell, B., Larkin, S., Mu, Y., Gage, F.H. Dopaminergic differentiation of human pluripotent cells. (2012) Curr Protoc Stem Cell Biol. Chapter 1:Unit1H.6. DOI: 10.1002/9780470151808.sc01h06s22

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Callaway, E.M., Marder, E. Common features of diverse circuits. (2012) Current Opinion in Neurobiology. 22(4):565-567. DOI: 10.1016/j.conb.2012.07.003

Egawa, N., Kitaoka, S., Tsukita, K., Naitoh, M., Takahashi, K., Yamamoto, T., Adachi, F., Kondo, T., Okita, K., Asaka, I., Aoi, T., Watanabe, A., Yamada, Y., Morizane, A., Takahashi, J., Ayaki, T., Ito, H., Yoshikawa, K., Yamawaki, S., Suzuki, S., Watanabe, D., Hioki, H., Kaneko, T., Makioka, K., Okamoto, K., Takuma, H., Tamaoka, A., Hasegawa, K., Nonaka, T., Hasegawa, M., Kawata, A., Yoshida, M., Nakahata, T., Takahashi, R., Marchetto, M.C., Gage, F.H., Yamanaka, S., Inoue, H. Drug screening for ALS using patient-specific induced pluripotent stem cells. (2012) Medicina Traslacional en Ciencia. 4(145):145ra104. DOI: 10.1126/scitranslmed.3004052

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Macfarlan, T.S., Gifford, W.D., Driscoll, S., Lettieri, K., Rowe, H.M., Bonanomi, D., Firth, A., Singer, O., Trono, D., Pfaff, S.L. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. (2012) Naturaleza. 487(7405):57-63. DOI: 10.1038/nature11244

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Giorgetti, A., Marchetto, M.C., Li, M., Yu, D., Fazzina, R., Mu, Y., Adamo, A., Paramonov, I., Cardoso, J.C., Monasterio, M.B., Bardy, C., Cassiani-Ingoni, R., Liu, G.H., Gage, F.H., Izpisua Belmonte, J.C. Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. (2012) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 109(31):12556-61. DOI: 10.1073/pnas.1209523109

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Nielsen, K.J., Callaway, E.M., Krauzlis, R.J. Viral vector-based reversible neuronal inactivation and behavioral manipulation in the macaque monkey. (2012) Frontiers in Systems Neuroscience. 6:48. DOI: 10.3389/fnsys.2012.00048

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Marchetto, M.C., Gage, F.H. Modeling brain disease in a dish: really? (2012) Células Madre. 10(6):642-5. DOI: 10.1016/j.stem.2012.05.008

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Zhao, C., Warner-Schmidt, J., Duman, R.S., Gage, F.H. Electroconvulsive seizure promotes spine maturation in newborn dentate granule cells in adult rat. (2012) Developmental Neurobiology. 72(6):937-42. DOI: 10.1002/dneu.20986

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Atencio, C.A., Sharpee, T.O., Schreiner, C.E. Receptive field dimensionality increases from the auditory midbrain to cortex. (2012) Journal of Neurophysiology. 107(10):2594-603. DOI: 10.1152/jn.01025.2011

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Nimmerjahn, A. Two-photon imaging of microglia in the mouse cortex in vivo. (2012) Protocolos de Cold Spring Harbor. 2012(5). DOI: 10.1101/pdb.prot069294

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Nimmerjahn, A. Optical window preparation for two-photon imaging of microglia in mice. (2012) Protocolos de Cold Spring Harbor. 2012(5). DOI: 10.1101/pdb.prot069286

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Nimmerjahn, A. Surgical implantation of a head plate in mice in preparation for in vivo two-photon imaging of microglia. (2012) Protocolos de Cold Spring Harbor. 2012(5). DOI: 10.1101/pdb.prot069278

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Yao, J., Mu, Y., Gage, F.H. Neural stem cells: mechanisms and modeling. (2012) Proteínas y Células. 3(4):251-61. DOI: 10.1007/s13238-012-2033-6

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Bonjean, M., Baker, T., Bazhenov, M., Cash, S., Halgren, E., Sejnowski, T. Interactions between core and matrix thalamocortical projections in human sleep spindle synchronization. (2012) Diario de Neurociencia. 32(15):5250-5263. DOI: 10.1523/JNEUROSCI.6141-11.2012

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Tanaka, H., Krakauer, J.W., Sejnowski, T.J. Generalization and multirate models of motor adaptation. (2012) Computación neuronal. 24(4):939-966. DOI: 10.1162/NECO_a_00262

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Gage, F.H. Transplantation in the future. (2012) Progress in Brain Research. 200:7-13. DOI: 10.1016/B978-0-444-59575-1.00001-6

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Gage, F.H., Muotri, A.R. What makes each brain unique. (2012) Sci. Am. 306(3):26-31.

Li, Y., Aimone, J.B., Xu, X., Callaway, E.M., Gage, F.H. Development of GABAergic inputs controls the contribution of maturing neurons to the adult hippocampal network. (2012) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 109(11):4290-5. DOI: 10.1073/pnas.1120754109

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Nimmerjahn, A., Helmchen, F. In vivo labeling of cortical astrocytes with sulforhodamine 101 (SR101). (2012) Protocolos de Cold Spring Harbor. 2012(3):326-34. DOI: 10.1101/pdb.prot068155

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Ramirez-Moreno, D.F., Sejnowski, T.J. A computational model for the modulation of the prepulse inhibition of the acoustic startle reflex. (2012) Biol Cybern. 106(3):169-176. DOI: 10.1007/s00422-012-0485-7

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Powell, S.B., Sejnowski, T.J., Behrens, M.M. Behavioral and neurochemical consequences of cortical oxidative stress on parvalbumin-interneuron maturation in rodent models of schizophrenia. (2012) Neuropharmacology. 62(3):1322-31. DOI: 10.1016/j.neuropharm.2011.01.049

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Ge, W.P., Miyawaki, A., Gage, F.H., Jan, Y.N., Jan, L.Y. Local generation of glia is a major astrocyte source in postnatal cortex. (2012) Naturaleza. 484(7394):376-80. DOI: 10.1038/nature10959

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Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G.D., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F.H., Jessberger, S. Gene expression profiling of neural stem cells and their neuronal progeny reveals IGF2 as a regulator of adult hippocampal neurogenesis. (2012) Diario de Neurociencia. 32(10):3376-87. DOI: 10.1523/JNEUROSCI.4248-11.2012

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Thuret, S., Thallmair, M., Horky, L.L., Gage, F.H. Enhanced functional recovery in MRL/MpJ mice after spinal cord dorsal hemisection. (2012) PLOS One. 7(2):e30904. DOI: 10.1371/journal.pone.0030904

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Bonanomi, D., Chivatakarn, O., Bai, G., Abdesselem, H., Lettieri, K., Marquardt, T., Pierchala, B.A., Pfaff, S.L. Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals. (2012) Móvil. 148(3):568-82. DOI: 10.1016/j.cell.2012.01.024

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Shimozaki, K., Zhang, C.L., Suh, H., Denli, A.M., Evans, R.M., Gage, F.H. SRY-box-containing gene 2 regulation of nuclear receptor tailless (Tlx) transcription in adult neural stem cells. (2012) Revista de Química Biológica. 287(8):5969-78. DOI: 10.1074/jbc.M111.290403

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Nauhaus, I., Nielsen, K.J., Callaway, E.M. Nonlinearity of two-photon Ca2+ imaging yields distorted measurements of tuning for V1 neuronal populations. (2012) Journal of Neurophysiology. 107(3):923-936. DOI: 10.1152/jn.00725.2011

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Lee, S.W., Clemenson, G.D., Gage, F.H. New neurons in an aged brain. (2012) Behavioural Brain Research. 227(2):497-507. DOI: 10.1016/j.bbr.2011.10.009

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Kerr, J.N., Nimmerjahn, A. Functional imaging in freely moving animals. (2012) Current Opinion in Neurobiology. 22(1):45-53. DOI: 10.1016/j.conb.2011.12.002

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Vivar, C., Potter, M.C., Choi, J., Lee, J.Y., Stringer, T.P., Callaway, E.M., Gage, F.H., Suh, H., van Praag, H. Monosynaptic inputs to new neurons in the dentate gyrus. (2012) Nature Communications. 3:1107. DOI: 10.1038/ncomms2101

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Sharpee, T.O. Adaptive switches in midbrain circuits. (2012) Neurona. 73(1):6-7. DOI: 10.1016/j.neuron.2011.12.017

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Gage, F.H. ISSCR: beginning the next chapter. (2012) Células Madre. 10(1):29-31. DOI: 10.1016/j.stem.2011.12.009

Han, J., Denli, A.M., Gage, F.H. The enemy within: intronic miR-26b represses its host gene, ctdsp2, to regulate neurogenesis. (2012) Genes y Desarrollo. 26(1):6-10. DOI: 10.1101/gad.184416.111

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Stam, F.J., Hendricks, T.J., Zhang, J., Geiman, E.J., Francius, C., Labosky, P.A., Clotman, F., Goulding, M. Renshaw cell interneuron specialization is controlled by a temporally restricted transcription factor program. (2012) Development. 139(1):179-90. DOI: 10.1242/dev.071134

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Brennand, K.J., Gage, F.H. Modeling psychiatric disorders through reprogramming. (2012) Dis Model Mech. 5(1):26-32. DOI: 10.1242/dmm.008268

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Nhan, H.L., Callaway, E.M. Morphology of superior colliculus- and middle temporal area-projecting neurons in primate primary visual cortex. (2012) Journal of Comparative Neurology. 520(1):52-80. DOI: 10.1002/cne.22685

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Ikrar, T., Shi, Y., Velasquez, T., Goulding, M., Xu, X. Cell-type specific regulation of cortical excitability through the allatostatin receptor system. (2012) Front Neural Circuits. 6:2. DOI: 10.3389/fncir.2012.00002

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Israel, M.A., Yuan, S.H., Bardy, C., Reyna, S.M., Mu, Y., Herrera, C., Hefferan, M.P., Van Gorp, S., Nazor, K.L., Boscolo, F.S., Carson, C.T., Laurent, L.C., Marsala, M., Gage, F.H., Remes, A.M., Koo, E.H., Goldstein, L.S. Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells. (2012) Naturaleza. 482(7384):216-20. DOI: 10.1038/nature10821

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Our understanding of Alzheimer's disease pathogenesis is currently limited by difficulties in obtaining live neurons from patients and the inability to model the sporadic form of the disease. It may be possible to overcome these challenges by reprogramming primary cells from patients into induced pluripotent stem cells (iPSCs). Here we reprogrammed primary fibroblasts from two patients with familial Alzheimer's disease, both caused by a duplication of the amyloid-β precursor protein gene (APP; termed APP(Dp)), two with sporadic Alzheimer's disease (termed sAD1, sAD2) and two non-demented control individuals into iPSC lines. Neurons from differentiated cultures were purified with fluorescence-activated cell sorting and characterized. Purified cultures contained more than 90% neurons, clustered with fetal brain messenger RNA samples by microarray criteria, and could form functional synaptic contacts. Virtually all cells exhibited normal electrophysiological activity. Relative to controls, iPSC-derived, purified neurons from the two APP(Dp) patients and patient sAD2 exhibited significantly higher levels of the pathological markers amyloid-β(1-40), phospho-tau(Thr 231) and active glycogen synthase kinase-3β (aGSK-3β). Neurons from APP(Dp) and sAD2 patients also accumulated large RAB5-positive early endosomes compared to controls. Treatment of purified neurons with β-secretase inhibitors, but not γ-secretase inhibitors, caused significant reductions in phospho-Tau(Thr 231) and aGSK-3β levels. These results suggest a direct relationship between APP proteolytic processing, but not amyloid-β, in GSK-3β activation and tau phosphorylation in human neurons. Additionally, we observed that neurons with the genome of one sAD patient exhibited the phenotypes seen in familial Alzheimer's disease samples. More generally, we demonstrate that iPSC technology can be used to observe phenotypes relevant to Alzheimer's disease, even though it can take decades for overt disease to manifest in patients.

Boyce, V.S., Park, J., Gage, F.H., Mendell, L.M. Differential effects of brain-derived neurotrophic factor and neurotrophin-3 on hindlimb function in paraplegic rats. (2012) Eur. J. Neurosci. 35(2):221-32. DOI: 10.1111/j.1460-9568.2011.07950.x

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We compared the effect of viral administration of brain-derived neurotrophic factor (BDNF) or neurotrophin 3 (NT-3) on locomotor recovery in adult rats with complete thoracic (T10) spinal cord transection injuries, in order to determine the effect of chronic neurotrophin expression on spinal plasticity. At the time of injury, BDNF, NT-3 or green fluorescent protein (GFP) (control) was delivered to the lesion via adeno-associated virus (AAV) constructs. AAV-BDNF was significantly more effective than AAV-NT-3 in eliciting locomotion. In fact, AAV-BDNF-treated rats displayed plantar, weight-supported hindlimb stepping on a stationary platform, that is, without the assistance of a moving treadmill and without step training. Rats receiving AAV-NT-3 or AAV-GFP were incapable of hindlimb stepping during this task, despite provision of balance support. AAV-NT-3 treatment did promote the recovery of treadmill-assisted stepping, but this required continuous perineal stimulation. In addition, AAV-BDNF-treated rats were sensitized to noxious heat, whereas AAV-NT-3-treated and AAV-GFP-treated rats were not. Notably, AAV-BDNF-treated rats also developed hindlimb spasticity, detracting from its potential clinical applicability via the current viral delivery method. Intracellular recording from triceps surae motoneurons revealed that AAV-BDNF significantly reduced motoneuron rheobase, suggesting that AAV-BDNF promoted the recovery of over-ground stepping by enhancing neuronal excitability. Elevated nuclear c-Fos expression in interneurons located in the L2 intermediate zone after AAV-BDNF treatment indicated increased activation of interneurons in the vicinity of the locomotor central pattern generator. AAV-NT-3 treatment reduced motoneuron excitability, with little change in c-Fos expression. These results support the potential for BDNF delivery at the lesion site to reorganize locomotor circuits.

Marshel, J.H., Garrett, M.E., Nauhaus, I., Callaway, E.M. Functional specialization of seven mouse visual cortical areas. (2011) Neurona. 72(6):1040-1054. DOI: 10.1016/j.neuron.2011.12.004

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Mu, Y., Gage, F.H. Adult hippocampal neurogenesis and its role in Alzheimer's disease. (2011) Mol Neurodegener. 6:85. DOI: 10.1186/1750-1326-6-85

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Volman, V., Behrens, M.M., Sejnowski, T.J. Downregulation of parvalbumin at cortical GABA synapses reduces network gamma oscillatory activity. (2011) Diario de Neurociencia. 31(49):18137-48. DOI: 10.1523/JNEUROSCI.3041-11.2011

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Coufal, N.G., Garcia-Perez, J.L., Peng, G.E., Marchetto, M.C., Muotri, A.R., Mu, Y., Carson, C.T., Macia, A., Moran, J.V., Gage, F.H. Ataxia telangiectasia mutated (ATM) modulates long interspersed element-1 (L1) retrotransposition in human neural stem cells. (2011) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 108(51):20382-7. DOI: 10.1073/pnas.1100273108

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Coggan, J.S., Ocker, G.K., Sejnowski, T.J., Prescott, S.A. Explaining pathological changes in axonal excitability through dynamical analysis of conductance-based models. (2011) J Neural Eng. 8(6):065002. DOI: 10.1088/1741-2560/8/6/065002

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Brennand, K.J., Gage, F.H. Concise review: the promise of human induced pluripotent stem cell-based studies of schizophrenia. (2011) Células madre. 29(12):1915-22. DOI: 10.1002/stem.762

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Choi, J., Callaway, E.M. Monosynaptic inputs to ErbB4-expressing inhibitory neurons in mouse primary somatosensory cortex. (2011) Journal of Comparative Neurology. 519(17):3402-3414. DOI: 10.1002/cne.22680

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Kuwabara, T., Kagalwala, M.N., Onuma, Y., Ito, Y., Warashina, M., Terashima, K., Sanosaka, T., Nakashima, K., Gage, F.H., Asashima, M. Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb. (2011) EMBO Molecular Medicine. 3(12):742-54. DOI: 10.1002/emmm.201100177

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Tremblay, M., Stevens, B., Sierra, A., Wake, H., Bessis, A., Nimmerjahn, A. The role of microglia in the healthy brain. (2011) Diario de Neurociencia. 31(45):16064-9. DOI: 10.1523/JNEUROSCI.4158-11.2011

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Wei, H., Bonjean, M., Petry, H.M., Sejnowski, T.J., Bickford, M.E. Thalamic burst firing propensity: a comparison of the dorsal lateral geniculate and pulvinar nuclei in the tree shrew. (2011) Diario de Neurociencia. 31(47):17287-17299. DOI: 10.1523/JNEUROSCI.6431-10.2011

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Brenner, S., Sejnowski, T.J. Understanding the human brain. (2011) Ciencia. 334(6056):567. DOI: 10.1126/science.1215674

Fitzgerald, J.D., Rowekamp, R.J., Sincich, L.C., Sharpee, T.O. Second order dimensionality reduction using minimum and maximum mutual information models. (2011) PLOS Biología Computacional. 7(10):e1002249. DOI: 10.1371/journal.pcbi.1002249

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Bai, G., Pfaff, S.L. Protease regulation: the Yin and Yang of neural development and disease. (2011) Neurona. 72(1):9-21. DOI: 10.1016/j.neuron.2011.09.012

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Kawakami, Y., Marti, M., Kawakami, H., Itou, J., Quach, T., Johnson, A., Sahara, S., O'Leary, D.D., Nakagawa, Y., Lewandoski, M., Pfaff, S., Evans, S.M., Izpisua Belmonte, J.C. Islet1-mediated activation of the β-catenin pathway is necessary for hindlimb initiation in mice. (2011) Development. 138(20):4465-73. DOI: 10.1242/dev.065359

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Marchetto, M.C., Brennand, K.J., Boyer, L.F., Gage, F.H. Induced pluripotent stem cells (iPSCs) and neurological disease modeling: progress and promises. (2011) Genética Molecular Humana. 20(R2):R109-15. DOI: 10.1093/hmg/ddr336

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Volman, V., Sejnowski, T.J., Bazhenov, M. Topological basis of epileptogenesis in a model of severe cortical trauma. (2011) Journal of Neurophysiology. 106(4):1933-1942. DOI: 10.1152/jn.00458.2011

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Sharpee, T.O., Nagel, K.I., Doupe, A.J. Two-dimensional adaptation in the auditory forebrain. (2011) Journal of Neurophysiology. 106(4):1841-61. DOI: 10.1152/jn.00905.2010

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Sharpee, T.O., Atencio, C.A., Schreiner, C.E. Hierarchical representations in the auditory cortex. (2011) Current Opinion in Neurobiology. 21(5):761-7. DOI: 10.1016/j.conb.2011.05.027

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Hester, M.E., Murtha, M.J., Song, S., Rao, M., Miranda, C.J., Meyer, K., Tian, J., Boulting, G., Schaffer, D.V., Zhu, M.X., Pfaff, S.L., Gage, F.H., Kaspar, B.K. Rapid and efficient generation of functional motor neurons from human pluripotent stem cells using gene delivered transcription factor codes. (2011) Mol. Ther. 19(10):1905-12. DOI: 10.1038/mt.2011.135

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Yu, T., Sejnowski, T.J., Cauwenberghs, G. Biophysical Neural Spiking, Bursting, and Excitability Dynamics in Reconfigurable Analog VLSI. (2011) IEEE Trans Biomed Circuits Syst. 5(5):420-429. DOI: 10.1109/TBCAS.2011.2169794

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Zhu, Q., Pao, G.M., Huynh, A.M., Suh, H., Tonnu, N., Nederlof, P.M., Gage, F.H., Verma, I.M. BRCA1 tumour suppression occurs via heterochromatin-mediated silencing. (2011) Naturaleza. 477(7363):179-84. DOI: 10.1038/nature10371

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Volman, V., Bazhenov, M., Sejnowski, T.J. Pattern of trauma determines the threshold for epileptic activity in a model of cortical deafferentation. (2011) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 108(37):15402-15407. DOI: 10.1073/pnas.1112066108

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Ghosh, K.K., Burns, L.D., Cocker, E.D., Nimmerjahn, A., Ziv, Y., Gamal, A.E., Schnitzer, M.J. Miniaturized integration of a fluorescence microscope. (2011) Nature Methods. 8(10):871-8. DOI: 10.1038/nmeth.1694

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Dang-Vu, T.T., Bonjean, M., Schabus, M., Boly, M., Darsaud, A., Desseilles, M., Degueldre, C., Balteau, E., Phillips, C., Luxen, A., Sejnowski, T.J., Maquet, P. Interplay between spontaneous and induced brain activity during human non-rapid eye movement sleep. (2011) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 108(37):15438-15443. DOI: 10.1073/pnas.1112503108

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Toups, J.V., Fellous, J.M., Thomas, P.J., Sejnowski, T.J., Tiesinga, P.H. Finding the event structure of neuronal spike trains. (2011) Computación neuronal. 23(9):2169-2208. DOI: 10.1162/NECO_a_00173

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Osakada, F., Mori, T., Cetin, A.H., Marshel, J.H., Virgen, B., Callaway, E.M. New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. (2011) Neurona. 71(4):617-631. DOI: 10.1016/j.neuron.2011.07.005

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Shen, B., Xiang, Z., Miller, B., Louie, G., Wang, W., Noel, J.P., Gage, F.H., Wang, L. Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons. (2011) Células madre. 29(8):1231-40. DOI: 10.1002/stem.679

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Rowekamp, R.J., Sharpee, T.O. Analyzing multicomponent receptive fields from neural responses to natural stimuli. (2011) Network. 22(1-4):45-73. DOI: 10.3109/0954898X.2011.566303

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Alaynick, W.A., Jessell, T.M., Pfaff, S.L. SnapShot: spinal cord development. (2011) Móvil. 146(1):178-178.e1. DOI: 10.1016/j.cell.2011.06.038

Woods, N.B., Parker, A.S., Moraghebi, R., Lutz, M.K., Firth, A.L., Brennand, K.J., Berggren, W.T., Raya, A., Izpisúa Belmonte, J.C., Gage, F.H., Verma, I.M. Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines. (2011) Células madre. 29(7):1158-64. DOI: 10.1002/stem.657

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Liang, X., Song, M.R., Xu, Z., Lanuza, G.M., Liu, Y., Zhuang, T., Chen, Y., Pfaff, S.L., Evans, S.M., Sun, Y. Isl1 is required for multiple aspects of motor neuron development. (2011) Molecular and Cellular Neurosciences. 47(3):215-22. DOI: 10.1016/j.mcn.2011.04.007

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Bonjean, M., Baker, T., Lemieux, M., Timofeev, I., Sejnowski, T., Bazhenov, M. Corticothalamic feedback controls sleep spindle duration in vivo. (2011) Diario de Neurociencia. 31(25):9124-9134. DOI: 10.1523/JNEUROSCI.0077-11.2011

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Aimone, J.B., Deng, W., Gage, F.H. Resolving new memories: a critical look at the dentate gyrus, adult neurogenesis, and pattern separation. (2011) Neurona. 70(4):589-96. DOI: 10.1016/j.neuron.2011.05.010

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Callaway, E.M., Borrell, V. Developmental sculpting of dendritic morphology of layer 4 neurons in visual cortex: influence of retinal input. (2011) Diario de Neurociencia. 31(20):7456-7470. DOI: 10.1523/JNEUROSCI.5222-10.2011

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Brennand, K.J., Simone, A., Jou, J., Gelboin-Burkhart, C., Tran, N., Sangar, S., Li, Y., Mu, Y., Chen, G., Yu, D., McCarthy, S., Sebat, J., Gage, F.H. Modelling schizophrenia using human induced pluripotent stem cells. (2011) Naturaleza. 473(7346):221-5. DOI: 10.1038/nature09915

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Bruno, I.G., Karam, R., Huang, L., Bhardwaj, A., Lou, C.H., Shum, E.Y., Song, H.W., Corbett, M.A., Gifford, W.D., Gecz, J., Pfaff, S.L., Wilkinson, M.F. Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay. (2011) Biología Celular Molecular. 42(4):500-10. DOI: 10.1016/j.molcel.2011.04.018

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Miyamichi, K., Amat, F., Moussavi, F., Wang, C., Wickersham, I., Wall, N.R., Taniguchi, H., Tasic, B., Huang, Z.J., He, Z., Callaway, E.M., Horowitz, M.A., Luo, L. Cortical representations of olfactory input by trans-synaptic tracing. (2011) Naturaleza. 472(7342):191-196. DOI: 10.1038/nature09714

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Fitzgerald, J.D., Sincich, L.C., Sharpee, T.O. Minimal models of multidimensional computations. (2011) PLOS Biología Computacional. 7(3):e1001111. DOI: 10.1371/journal.pcbi.1001111

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Mu, Y., Zhao, C., Gage, F.H. Dopaminergic modulation of cortical inputs during maturation of adult-born dentate granule cells. (2011) Diario de Neurociencia. 31(11):4113-23. DOI: 10.1523/JNEUROSCI.4913-10.2011

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Aimone, J.B., Gage, F.H. Modeling new neuron function: a history of using computational neuroscience to study adult neurogenesis. (2011) European Journal of Neuroscience. 33(6):1160-9. DOI: 10.1111/j.1460-9568.2011.07615.x

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Winner, B., Kohl, Z., Gage, F.H. Neurodegenerative disease and adult neurogenesis. (2011) Eur. J. Neurosci. 33(6):1139-51. DOI: 10.1111/j.1460-9568.2011.07613.x

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Macfarlan, T.S., Gifford, W.D., Agarwal, S., Driscoll, S., Lettieri, K., Wang, J., Andrews, S.E., Franco, L., Rosenfeld, M.G., Ren, B., Pfaff, S.L. Endogenous retroviruses and neighboring genes are coordinately repressed by LSD1/KDM1A. (2011) Genes y Desarrollo. 25(6):594-607. DOI: 10.1101/gad.2008511

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Winner, B., Jappelli, R., Maji, S.K., Desplats, P.A., Boyer, L., Aigner, S., Hetzer, C., Loher, T., Vilar, M., Campioni, S., Tzitzilonis, C., Soragni, A., Jessberger, S., Mira, H., Consiglio, A., Pham, E., Masliah, E., Gage, F.H., Riek, R. In vivo demonstration that alpha-synuclein oligomers are toxic. (2011) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 108(10):4194-9. DOI: 10.1073/pnas.1100976108

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Winner, B., Melrose, H.L., Zhao, C., Hinkle, K.M., Yue, M., Kent, C., Braithwaite, A.T., Ogholikhan, S., Aigner, R., Winkler, J., Farrer, M.J., Gage, F.H. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice. (2011) Neurobiol. Dis. 41(3):706-16. DOI: 10.1016/j.nbd.2010.12.008

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Romani, S., Sejnowski, T.J., Tsodyks, M. Intracellular dynamics of virtual place cells. (2011) Computación neuronal. 23(3):651-5. DOI: 10.1162/NECO_a_00087

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Yuan, S.H., Martin, J., Elia, J., Flippin, J., Paramban, R.I., Hefferan, M.P., Vidal, J.G., Mu, Y., Killian, R.L., Israel, M.A., Emre, N., Marsala, S., Marsala, M., Gage, F.H., Goldstein, L.S., Carson, C.T. Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells. (2011) PLOS One. 6(3):e17540. DOI: 10.1371/journal.pone.0017540

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Garudadri, S., Gallarda, B., Pfaff, S., Alaynick, W. Spinal cord electrophysiology II: extracellular suction electrode fabrication. (2011) J Vis Exp.(48) DOI: 10.3791/2580

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Jeanne, J.M., Thompson, J.V., Sharpee, T.O., Gentner, T.Q. Emergence of learned categorical representations within an auditory forebrain circuit. (2011) Diario de Neurociencia. 31(7):2595-606. DOI: 10.1523/JNEUROSCI.3930-10.2011

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Kim, J.E., O'Sullivan, M.L., Sanchez, C.A., Hwang, M., Israel, M.A., Brennand, K., Deerinck, T.J., Goldstein, L.S., Gage, F.H., Ellisman, M.H., Ghosh, A. Investigating synapse formation and function using human pluripotent stem cell-derived neurons. (2011) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 108(7):3005-10. DOI: 10.1073/pnas.1007753108

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Bai, G., Chivatakarn, O., Bonanomi, D., Lettieri, K., Franco, L., Xia, C., Stein, E., Ma, L., Lewcock, J.W., Pfaff, S.L. Presenilin-dependent receptor processing is required for axon guidance. (2011) Móvil. 144(1):106-18. DOI: 10.1016/j.cell.2010.11.053

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Meng, X., Arocena, M., Penninger, J., Gage, F.H., Zhao, M., Song, B. PI3K mediated electrotaxis of embryonic and adult neural progenitor cells in the presence of growth factors. (2011) Exp. Neurol. 227(1):210-7. DOI: 10.1016/j.expneurol.2010.11.002

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Peterson, D.A., Lotz, D.T., Halgren, E., Sejnowski, T.J., Poizner, H. Choice modulates the neural dynamics of prediction error processing during rewarded learning. (2011) Neuroimage. 54(2):1385-1394. DOI: 10.1016/j.neuroimage.2010.09.051

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Ruiz, S., Brennand, K., Panopoulos, A.D., Herrerías, A., Gage, F.H., Izpisua-Belmonte, J.C. High-efficient generation of induced pluripotent stem cells from human astrocytes. (2010) PLOS One. 5(12):e15526. DOI: 10.1371/journal.pone.0015526

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Wall, N.R., Wickersham, I.R., Cetin, A., De La Parra, M., Callaway, E.M. Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(50):21848-21853. DOI: 10.1073/pnas.1011756107

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Timofeev, I., Bazhenov, M., Seigneur, J., Sejnowski, T. Neocortical synchronization. (2010) Epilepsia. 51(Suppl 5):18. DOI: 10.1111/j.1528-1167.2010.02804.x

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Weible, A.P., Schwarcz, L., Wickersham, I.R., Deblander, L., Wu, H., Callaway, E.M., Seung, H.S., Kentros, C.G. Transgenic targeting of recombinant rabies virus reveals monosynaptic connectivity of specific neurons. (2010) Diario de Neurociencia. 30(49):16509-16513. DOI: 10.1523/JNEUROSCI.2442-10.2010

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Hill, E.S., Moore-Kochlacs, C., Vasireddi, S.K., Sejnowski, T.J., Frost, W.N. Validation of independent component analysis for rapid spike sorting of optical recording data. (2010) Journal of Neurophysiology. 104(6):3721-3731. DOI: 10.1152/jn.00691.2010

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Sejnowski, T.J. Learning optimal strategies in complex environments. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(47):20151-2. DOI: 10.1073/pnas.1014954107

Marchetto, M.C., Carromeu, C., Acab, A., Yu, D., Yeo, G.W., Mu, Y., Chen, G., Gage, F.H., Muotri, A.R. A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells. (2010) Móvil. 143(4):527-39. DOI: 10.1016/j.cell.2010.10.016

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Coggan, J.S., Prescott, S.A., Bartol, T.M., Sejnowski, T.J. Imbalance of ionic conductances contributes to diverse symptoms of demyelination. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(48):20602-20609. DOI: 10.1073/pnas.1013798107

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Mathews, E.A., Morgenstern, N.A., Piatti, V.C., Zhao, C., Jessberger, S., Schinder, A.F., Gage, F.H. A distinctive layering pattern of mouse dentate granule cells is generated by developmental and adult neurogenesis. (2010) Journal of Comparative Neurology. 518(22):4479-90. DOI: 10.1002/cne.22489

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Heider, B., Nathanson, J.L., Isacoff, E.Y., Callaway, E.M., Siegel, R.M. Two-photon imaging of calcium in virally transfected striate cortical neurons of behaving monkey. (2010) PLOS One. 5(11). DOI: 10.1371/journal.pone.0013829

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Haubensak, W., Kunwar, P.S., Cai, H., Ciocchi, S., Wall, N.R., Ponnusamy, R., Biag, J., Dong, H.W., Deisseroth, K., Callaway, E.M., Fanselow, M.S., Lüthi, A., Anderson, D.J. Genetic dissection of an amygdala microcircuit that gates conditioned fear. (2010) Naturaleza. 468(7321):270-276. DOI: 10.1038/nature09553

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Tiesinga, P.H., Sejnowski, T.J. Mechanisms for Phase Shifting in Cortical Networks and their Role in Communication through Coherence. (2010) Frontiers in Human Neuroscience. 4:196. DOI: 10.3389/fnhum.2010.00196

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Nadkarni, S., Bartol, T.M., Sejnowski, T.J., Levine, H. Modelling vesicular release at hippocampal synapses. (2010) PLOS Biología Computacional. 6(11):e1000983. DOI: 10.1371/journal.pcbi.1000983

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Volman, V., Levine, H., Sejnowski, T.J. Shunting inhibition controls the gain modulation mediated by asynchronous neurotransmitter release in early development. (2010) PLOS Biología Computacional. 6(11):e1000973. DOI: 10.1371/journal.pcbi.1000973

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Sejnowski, T.J. QnAs with Terrence J. Sejnowski. [Interviewed by Prashant Nair]. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(48):20601. DOI: 10.1073/pnas.1015459107

Muotri, A.R., Marchetto, M.C., Coufal, N.G., Oefner, R., Yeo, G., Nakashima, K., Gage, F.H. L1 retrotransposition in neurons is modulated by MeCP2. (2010) Naturaleza. 468(7322):443-6. DOI: 10.1038/nature09544

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Long interspersed nuclear elements-1 (LINE-1 or L1s) are abundant retrotransposons that comprise approximately 20% of mammalian genomes. Active L1 retrotransposons can impact the genome in a variety of ways, creating insertions, deletions, new splice sites or gene expression fine-tuning. We have shown previously that L1 retrotransposons are capable of mobilization in neuronal progenitor cells from rodents and humans and evidence of massive L1 insertions was observed in adult brain tissues but not in other somatic tissues. In addition, L1 mobility in the adult hippocampus can be influenced by the environment. The neuronal specificity of somatic L1 retrotransposition in neural progenitors is partially due to the transition of a Sox2/HDAC1 repressor complex to a Wnt-mediated T-cell factor/lymphoid enhancer factor (TCF/LEF) transcriptional activator. The transcriptional switch accompanies chromatin remodelling during neuronal differentiation, allowing a transient stimulation of L1 transcription. The activity of L1 retrotransposons during brain development can have an impact on gene expression and neuronal function, thereby increasing brain-specific genetic mosaicism. Further understanding of the molecular mechanisms that regulate L1 expression should provide new insights into the role of L1 retrotransposition during brain development. Here we show that L1 neuronal transcription and retrotransposition in rodents are increased in the absence of methyl-CpG-binding protein 2 (MeCP2), a protein involved in global DNA methylation and human neurodevelopmental diseases. Using neuronal progenitor cells derived from human induced pluripotent stem cells and human tissues, we revealed that patients with Rett syndrome (RTT), carrying MeCP2 mutations, have increased susceptibility for L1 retrotransposition. Our data demonstrate that L1 retrotransposition can be controlled in a tissue-specific manner and that disease-related genetic mutations can influence the frequency of neuronal L1 retrotransposition. Our findings add a new level of complexity to the molecular events that can lead to neurological disorders.

Ma, D.K., Marchetto, M.C., Guo, J.U., Ming, G.L., Gage, F.H., Song, H. Epigenetic choreographers of neurogenesis in the adult mammalian brain. (2010) Nature Neuroscience. 13(11):1338-44. DOI: 10.1038/nn.2672

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Aimone, J.B., Deng, W., Gage, F.H. Put them out to pasture? What are old granule cells good for, anyway...? (2010) Hipocampo. 20(10):1124-5. DOI: 10.1002/hipo.20867

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Miller, K.J., Hermes, D., Honey, C.J., Sharma, M., Rao, R.P., den Nijs, M., Fetz, E.E., Sejnowski, T.J., Hebb, A.O., Ojemann, J.G., Makeig, S., Leuthardt, E.C. Dynamic modulation of local population activity by rhythm phase in human occipital cortex during a visual search task. (2010) Frontiers in Human Neuroscience. 4:197. DOI: 10.3389/fnhum.2010.00197

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Liu, D., Ou, L., Clemenson, G.D., Chao, C., Lutske, M.E., Zambetti, G.P., Gage, F.H., Xu, Y. Puma is required for p53-induced depletion of adult stem cells. (2010) Naturaleza y Biología Celular. 12(10):993-8. DOI: 10.1038/ncb2100

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Hasenstaub, A.R., Callaway, E.M. Paint it black (or red, or green): optical and genetic tools illuminate inhibitory contributions to cortical circuit function. (2010) Neurona. 67(5):681-684. DOI: 10.1016/j.neuron.2010.08.039

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Choi, J., Young, J.A., Callaway, E.M. Selective viral vector transduction of ErbB4 expressing cortical interneurons in vivo with a viral receptor-ligand bridge protein. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(38):16703-16708. DOI: 10.1073/pnas.1006233107

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Petruska, J.C., Kitay, B., Boyce, V.S., Kaspar, B.K., Pearse, D.D., Gage, F.H., Mendell, L.M. Intramuscular AAV delivery of NT-3 alters synaptic transmission to motoneurons in adult rats. (2010) Eur. J. Neurosci. 32(6):997-1005. DOI: 10.1111/j.1460-9568.2010.07392.x

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Hao, J., Lee, T.W., Sejnowski, T.J. Speech Enhancement Using Gaussian Scale Mixture Models. (2010) IEEE Trans Audio Speech Lang Process. 18(6):1127-1136. DOI: 10.1109/TASL.2009.2030012

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Marshel, J.H., Mori, T., Nielsen, K.J., Callaway, E.M. Targeting single neuronal networks for gene expression and cell labeling in vivo. (2010) Neurona. 67(4):562-574. DOI: 10.1016/j.neuron.2010.08.001

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Fröhlich, F., Sejnowski, T.J., Bazhenov, M. Network bistability mediates spontaneous transitions between normal and pathological brain states. (2010) Diario de Neurociencia. 30(32):10734-10743. DOI: 10.1523/JNEUROSCI.1239-10.2010

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Mu, Y., Lee, S.W., Gage, F.H. Signaling in adult neurogenesis. (2010) Current Opinion in Neurobiology. 20(4):416-23. DOI: 10.1016/j.conb.2010.04.010

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Singer, T., McConnell, M.J., Marchetto, M.C., Coufal, N.G., Gage, F.H. LINE-1 retrotransposons: mediators of somatic variation in neuronal genomes? (2010) Trends in Neurosciences. 33(8):345-54. DOI: 10.1016/j.tins.2010.04.001

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Valdez, G., Tapia, J.C., Kang, H., Clemenson, G.D., Gage, F.H., Lichtman, J.W., Sanes, J.R. Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(33):14863-8. DOI: 10.1073/pnas.1002220107

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Mira, H., Andreu, Z., Suh, H., Lie, D.C., Jessberger, S., Consiglio, A., San Emeterio, J., Hortigüela, R., Marqués-Torrejón, M.A., Nakashima, K., Colak, D., Götz, M., Fariñas, I., Gage, F.H. Signaling through BMPR-IA regulates quiescence and long-term activity of neural stem cells in the adult hippocampus. (2010) Células Madre. 7(1):78-89. DOI: 10.1016/j.stem.2010.04.016

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Hasenstaub, A., Otte, S., Callaway, E., Sejnowski, T.J. Metabolic cost as a unifying principle governing neuronal biophysics. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(27):12329-34. DOI: 10.1073/pnas.0914886107

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Aimone, J.B., Deng, W., Gage, F.H. Adult neurogenesis: integrating theories and separating functions. (2010) Trends in Cognitive Sciences. 14(7):325-37. DOI: 10.1016/j.tics.2010.04.003

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Imaizumi, K., Priebe, N.J., Sharpee, T.O., Cheung, S.W., Schreiner, C.E. Encoding of temporal information by timing, rate, and place in cat auditory cortex. (2010) PLOS One. 5(7):e11531. DOI: 10.1371/journal.pone.0011531

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Qu, Y., Glasco, D.M., Zhou, L., Sawant, A., Ravni, A., Fritzsch, B., Damrau, C., Murdoch, J.N., Evans, S., Pfaff, S.L., Formstone, C., Goffinet, A.M., Chandrasekhar, A., Tissir, F. Atypical cadherins Celsr1-3 differentially regulate migration of facial branchiomotor neurons in mice. (2010) Diario de Neurociencia. 30(28):9392-401. DOI: 10.1523/JNEUROSCI.0124-10.2010

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Nelson, J.D., McKenzie, C.R., Cottrell, G.W., Sejnowski, T.J. Experience matters: information acquisition optimizes probability gain. (2010) Psychol Sci. 21(7):960-969. DOI: 10.1177/0956797610372637

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Grossmann, K.S., Giraudin, A., Britz, O., Zhang, J., Goulding, M. Genetic dissection of rhythmic motor networks in mice. (2010) Progress in Brain Research. 187:19-37. DOI: 10.1016/B978-0-444-53613-6.00002-2

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Gallarda, B.W., Sharpee, T.O., Pfaff, S.L., Alaynick, W.A. Defining rhythmic locomotor burst patterns using a continuous wavelet transform. (2010) Ann. N. Y. Acad. Sci. 1198:133-9. DOI: 10.1111/j.1749-6632.2010.05437.x

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Hao, J., Lee, I., Lee, T.W., Sejnowski, T.J. Independent vector analysis for source separation using a mixture of gaussians prior. (2010) Computación neuronal. 22(6):1646-1673. DOI: 10.1162/neco.2010.11-08-906

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Deng, W., Aimone, J.B., Gage, F.H. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory? (2010) Nature Reviews Neuroscience. 11(5):339-50. DOI: 10.1038/nrn2822

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Moldakarimov, S., Bazhenov, M., Sejnowski, T.J. Representation sharpening can explain perceptual priming. (2010) Computación neuronal. 22(5):1312-32. DOI: 10.1162/neco.2009.04-09-999

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Zhang, M., Liu, J., Kim, Y., Dixon, J.E., Pfaff, S.L., Gill, G.N., Noel, J.P., Zhang, Y. Structural and functional analysis of the phosphoryl transfer reaction mediated by the human small C-terminal domain phosphatase, Scp1. (2010) Protein Science. 19(5):974-86. DOI: 10.1002/pro.375

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Modchang, C., Nadkarni, S., Bartol, T.M., Triampo, W., Sejnowski, T.J., Levine, H., Rappel, W.J. A comparison of deterministic and stochastic simulations of neuronal vesicle release models. (2010) Phys Biol. 7(2):026008. DOI: 10.1088/1478-3975/7/2/026008

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Marchetto, M.C., Winner, B., Gage, F.H. Pluripotent stem cells in neurodegenerative and neurodevelopmental diseases. (2010) Genética Molecular Humana. 19(R1):R71-6. DOI: 10.1093/hmg/ddq159

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Wang, H.P., Spencer, D., Fellous, J.M., Sejnowski, T.J. Synchrony of thalamocortical inputs maximizes cortical reliability. (2010) Ciencia. 328(5974):106-109. DOI: 10.1126/science.1183108

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Tronel, S., Fabre, A., Charrier, V., Oliet, S.H., Gage, F.H., Abrous, D.N. Spatial learning sculpts the dendritic arbor of adult-born hippocampal neurons. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(17):7963-8. DOI: 10.1073/pnas.0914613107

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Gage, F.H. Molecular and cellular mechanisms contributing to the regulation, proliferation and differentiation of neural stem cells in the adult dentate gyrus. (2010) Keio J Med. 59(3):79-83.

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Stiefel, K.M., Fellous, J.M., Thomas, P.J., Sejnowski, T.J. Intrinsic subthreshold oscillations extend the influence of inhibitory synaptic inputs on cortical pyramidal neurons. (2010) European Journal of Neuroscience. 31(6):1019-26. DOI: 10.1111/j.1460-9568.2010.07146.x

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Bonanomi, D., Pfaff, S.L. Motor axon pathfinding. (2010) Perspectivas de Cold Spring Harbor en Biología. 2(3):a001735. DOI: 10.1101/cshperspect.a001735

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Moldakarimov, S., Bazhenov, M., Sejnowski, T.J. Perceptual priming leads to reduction of gamma frequency oscillations. (2010) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 107(12):5640-5645. DOI: 10.1073/pnas.0907525107

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Drion, G., Bonjean, M., Waroux, O., Scuvée-Moreau, J., Liégeois, J.F., Sejnowski, T.J., Sepulchre, R., Seutin, V. M-type channels selectively control bursting in rat dopaminergic neurons. (2010) European Journal of Neuroscience. 31(5):827-835. DOI: 10.1111/j.1460-9568.2010.07107.x

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Peterson, D.A., Sejnowski, T.J., Poizner, H. Convergent evidence for abnormal striatal synaptic plasticity in dystonia. (2010) Neurobiol. Dis. 37(3):558-73. DOI: 10.1016/j.nbd.2009.12.003

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Dystonia is a functionally disabling movement disorder characterized by abnormal movements and postures. Although substantial recent progress has been made in identifying genetic factors, the pathophysiology of the disease remains a mystery. A provocative suggestion gaining broader acceptance is that some aspect of neural plasticity may be abnormal. There is also evidence that, at least in some forms of dystonia, sensorimotor "use" may be a contributing factor. Most empirical evidence of abnormal plasticity in dystonia comes from measures of sensorimotor cortical organization and physiology. However, the basal ganglia also play a critical role in sensorimotor function. Furthermore, the basal ganglia are prominently implicated in traditional models of dystonia, are the primary targets of stereotactic neurosurgical interventions, and provide a neural substrate for sensorimotor learning influenced by neuromodulators. Our working hypothesis is that abnormal plasticity in the basal ganglia is a critical link between the etiology and pathophysiology of dystonia. In this review we set up the background for this hypothesis by integrating a large body of disparate indirect evidence that dystonia may involve abnormalities in synaptic plasticity in the striatum. After reviewing evidence implicating the striatum in dystonia, we focus on the influence of two neuromodulatory systems: dopamine and acetylcholine. For both of these neuromodulators, we first describe the evidence for abnormalities in dystonia and then the means by which it may influence striatal synaptic plasticity. Collectively, the evidence suggests that many different forms of dystonia may involve abnormal plasticity in the striatum. An improved understanding of these altered plastic processes would help inform our understanding of the pathophysiology of dystonia, and, given the role of the striatum in sensorimotor learning, provide a principled basis for designing therapies aimed at the dynamic processes linking etiology to pathophysiology of the disease.

Glass, C.K., Saijo, K., Winner, B., Marchetto, M.C., Gage, F.H. Mechanisms underlying inflammation in neurodegeneration. (2010) Móvil. 140(6):918-34. DOI: 10.1016/j.cell.2010.02.016

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Gage, F. The promise and the challenge of modelling human disease in a dish. (2010) EMBO Molecular Medicine. 2(3):77-8. DOI: 10.1002/emmm.201000060

Otte, S., Hasenstaub, A., Callaway, E.M. Cell type-specific control of neuronal responsiveness by gamma-band oscillatory inhibition. (2010) Diario de Neurociencia. 30(6):2150-2159. DOI: 10.1523/JNEUROSCI.4818-09.2010

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Garcia-Campmany, L., Stam, F.J., Goulding, M. From circuits to behaviour: motor networks in vertebrates. (2010) Current Opinion in Neurobiology. 20(1):116-25. DOI: 10.1016/j.conb.2010.01.002

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Xu, X., Roby, K.D., Callaway, E.M. Immunochemical characterization of inhibitory mouse cortical neurons: three chemically distinct classes of inhibitory cells. (2010) Journal of Comparative Neurology. 518(3):389-404. DOI: 10.1002/cne.22229

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Lyon, D.C., Nassi, J.J., Callaway, E.M. A disynaptic relay from superior colliculus to dorsal stream visual cortex in macaque monkey. (2010) Neurona. 65(2):270-279. DOI: 10.1016/j.neuron.2010.01.003

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Meyer, A., Gallarda, B.W., Pfaff, S., Alaynick, W. Spinal cord electrophysiology. (2010) J Vis Exp.(35) DOI: 10.3791/1660

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Qu, Q., Sun, G., Li, W., Yang, S., Ye, P., Zhao, C., Yu, R.T., Gage, F.H., Evans, R.M., Shi, Y. Orphan nuclear receptor TLX activates Wnt/beta-catenin signalling to stimulate neural stem cell proliferation and self-renewal. (2010) Naturaleza y Biología Celular. 12(1):31-40; sup pp 1-9. DOI: 10.1038/ncb2001

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Sejnowski, T.J. Consequences of non-uniform active currents in dendrites. (2009) Fronteras en Neurociencia. 3(3):332-333. DOI: 10.3389/neuro.01.038.2009

Atencio, C.A., Sharpee, T.O., Schreiner, C.E. Hierarchical computation in the canonical auditory cortical circuit. (2009) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 106(51):21894-9. DOI: 10.1073/pnas.0908383106

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Volman, V., Levine, H., Ben-Jacob, E., Sejnowski, T.J. Locally balanced dendritic integration by short-term synaptic plasticity and active dendritic conductances. (2009) Journal of Neurophysiology. 102(6):3234-3250. DOI: 10.1152/jn.00260.2009

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Nathanson, J.L., Jappelli, R., Scheeff, E.D., Manning, G., Obata, K., Brenner, S., Callaway, E.M. Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. (2009) Front Neural Circuits. 3:19. DOI: 10.3389/neuro.04.019.2009

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Tanaka, H., Sejnowski, T.J., Krakauer, J.W. Adaptation to visuomotor rotation through interaction between posterior parietal and motor cortical areas. (2009) Journal of Neurophysiology. 102(5):2921-32. DOI: 10.1152/jn.90834.2008

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Nakashima, K., Kohyama, J., Namihira, M., Gage, F.H., Okano, H., Sawamoto, K. [Epigenetic mechanisms regulating neural cell fate determination]. (2009) No To Hattatsu. 41(6):411-4.

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Jessberger, S., Gage, F.H., Eisch, A.J., Lagace, D.C. Making a neuron: Cdk5 in embryonic and adult neurogenesis. (2009) Trends in Neurosciences. 32(11):575-82. DOI: 10.1016/j.tins.2009.07.002

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Peterson, D.A., Elliott, C., Song, D.D., Makeig, S., Sejnowski, T.J., Poizner, H. Probabilistic reversal learning is impaired in Parkinson's disease. (2009) Neuroscience. 163(4):1092-1101. DOI: 10.1016/j.neuroscience.2009.07.033

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Deng, W., Saxe, M.D., Gallina, I.S., Gage, F.H. Adult-born hippocampal dentate granule cells undergoing maturation modulate learning and memory in the brain. (2009) Diario de Neurociencia. 29(43):13532-42. DOI: 10.1523/JNEUROSCI.3362-09.2009

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Muotri, A.R., Zhao, C., Marchetto, M.C., Gage, F.H. Environmental influence on L1 retrotransposons in the adult hippocampus. (2009) Hipocampo. 19(10):1002-7. DOI: 10.1002/hipo.20564

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Wehr, M., Hostick, U., Kyweriga, M., Tan, A., Weible, A.P., Wu, H., Wu, W., Callaway, E.M., Kentros, C. Transgenic silencing of neurons in the mammalian brain by expression of the allatostatin receptor (AlstR). (2009) Journal of Neurophysiology. 102(4):2554-2562. DOI: 10.1152/jn.00480.2009

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Fitzgerald, J.D., Sharpee, T.O. Maximally informative pairwise interactions in networks. (2009) Phys Rev E Stat Nonlin Soft Matter Phys. 80(3 Pt 1):031914. DOI: 10.1103/PhysRevE.80.031914

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Liu, Y.S., Stevens, C.F., Sharpee, T.O. Predictable irregularities in retinal receptive fields. (2009) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 106(38):16499-504. DOI: 10.1073/pnas.0908926106

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Marchetto, M.C., Yeo, G.W., Kainohana, O., Marsala, M., Gage, F.H., Muotri, A.R. Transcriptional signature and memory retention of human-induced pluripotent stem cells. (2009) PLOS One. 4(9):e7076. DOI: 10.1371/journal.pone.0007076

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Behrens, M.M., Sejnowski, T.J. Does schizophrenia arise from oxidative dysregulation of parvalbumin-interneurons in the developing cortex? (2009) Neuropharmacology. 57(3):193-200. DOI: 10.1016/j.neuropharm.2009.06.002

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Lin, W., Metzakopian, E., Mavromatakis, Y.E., Gao, N., Balaskas, N., Sasaki, H., Briscoe, J., Whitsett, J.A., Goulding, M., Kaestner, K.H., Ang, S.L. Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development. (2009) Developmental Biology. 333(2):386-96. DOI: 10.1016/j.ydbio.2009.07.006

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Song, M.R., Sun, Y., Bryson, A., Gill, G.N., Evans, S.M., Pfaff, S.L. Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity. (2009) Development. 136(17):2923-32. DOI: 10.1242/dev.037986

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Mukamel, E.A., Nimmerjahn, A., Schnitzer, M.J. Automated analysis of cellular signals from large-scale calcium imaging data. (2009) Neurona. 63(6):747-60. DOI: 10.1016/j.neuron.2009.08.009

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Tiesinga, P., Sejnowski, T.J. Cortical enlightenment: are attentional gamma oscillations driven by ING or PING? (2009) Neurona. 63(6):727-732. DOI: 10.1016/j.neuron.2009.09.009

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Conner, J.M., Franks, K.M., Titterness, A.K., Russell, K., Merrill, D.A., Christie, B.R., Sejnowski, T.J., Tuszynski, M.H. NGF is essential for hippocampal plasticity and learning. (2009) Diario de Neurociencia. 29(35):10883-10889. DOI: 10.1523/JNEUROSCI.2594-09.2009

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Tamaki, S.J., Jacobs, Y., Dohse, M., Capela, A., Cooper, J.D., Reitsma, M., He, D., Tushinski, R., Belichenko, P.V., Salehi, A., Mobley, W., Gage, F.H., Huhn, S., Tsukamoto, A.S., Weissman, I.L., Uchida, N. Neuroprotection of host cells by human central nervous system stem cells in a mouse model of infantile neuronal ceroid lipofuscinosis. (2009) Células Madre. 5(3):310-9. DOI: 10.1016/j.stem.2009.05.022

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Kuwabara, T., Hsieh, J., Muotri, A., Yeo, G., Warashina, M., Lie, D.C., Moore, L., Nakashima, K., Asashima, M., Gage, F.H. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. (2009) Nature Neuroscience. 12(9):1097-105. DOI: 10.1038/nn.2360

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Coufal, N.G., Garcia-Perez, J.L., Peng, G.E., Yeo, G.W., Mu, Y., Lovci, M.T., Morell, M., O'Shea, K.S., Moran, J.V., Gage, F.H. L1 retrotransposition in human neural progenitor cells. (2009) Naturaleza. 460(7259):1127-31. DOI: 10.1038/nature08248

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Makeig, S., Gramann, K., Jung, T.P., Sejnowski, T.J., Poizner, H. Linking brain, mind and behavior. (2009) Int J Psychophysiol. 73(2):95-100. DOI: 10.1016/j.ijpsycho.2008.11.008

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Overall, R.W., Kempermann, G., Peirce, J., Lu, L., Goldowitz, D., Gage, F.H., Goodwin, S., Smit, A.B., Airey, D.C., Rosen, G.D., Schalkwyk, L.C., Sutter, T.R., Nowakowski, R.S., Whatley, S., Williams, R.W. Genetics of the hippocampal transcriptome in mouse: a systematic survey and online neurogenomics resource. (2009) Fronteras en Neurociencia. 3:55. DOI: 10.3389/neuro.15.003.2009

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Clelland, C.D., Choi, M., Romberg, C., Clemenson, G.D., Fragniere, A., Tyers, P., Jessberger, S., Saksida, L.M., Barker, R.A., Gage, F.H., Bussey, T.J. A functional role for adult hippocampal neurogenesis in spatial pattern separation. (2009) Ciencia. 325(5937):210-3. DOI: 10.1126/science.1173215

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Goulding, M. Circuits controlling vertebrate locomotion: moving in a new direction. (2009) Nature Reviews Neuroscience. 10(7):507-18. DOI: 10.1038/nrn2608

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Thuret, S., Toni, N., Aigner, S., Yeo, G.W., Gage, F.H. Hippocampus-dependent learning is associated with adult neurogenesis in MRL/MpJ mice. (2009) Hipocampo. 19(7):658-69. DOI: 10.1002/hipo.20550

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Destexhe, A., Sejnowski, T.J. The Wilson-Cowan model, 36 years later. (2009) Biol Cybern. 101(1):1-2. DOI: 10.1007/s00422-009-0328-3

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Meltzoff, A.N., Kuhl, P.K., Movellan, J., Sejnowski, T.J. Foundations for a new science of learning. (2009) Ciencia. 325(5938):284-288. DOI: 10.1126/science.1175626

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Fortun, J., Puzis, R., Pearse, D.D., Gage, F.H., Bunge, M.B. Muscle injection of AAV-NT3 promotes anatomical reorganization of CST axons and improves behavioral outcome following SCI. (2009) J. Neurotrauma. 26(7):941-53. DOI: 10.1089/neu.2008.0807

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Nathanson, J.L., Yanagawa, Y., Obata, K., Callaway, E.M. Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. (2009) Neuroscience. 161(2):441-450. DOI: 10.1016/j.neuroscience.2009.03.032

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Jagasia, R., Steib, K., Englberger, E., Herold, S., Faus-Kessler, T., Saxe, M., Gage, F.H., Song, H., Lie, D.C. GABA-cAMP response element-binding protein signaling regulates maturation and survival of newly generated neurons in the adult hippocampus. (2009) Diario de Neurociencia. 29(25):7966-77. DOI: 10.1523/JNEUROSCI.1054-09.2009

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Li, Y., Mu, Y., Gage, F.H. Development of neural circuits in the adult hippocampus. (2009) Current Topics in Developmental Biology. 87:149-74. DOI: 10.1016/S0070-2153(09)01205-8

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Sincich, L.C., Horton, J.C., Sharpee, T.O. Preserving information in neural transmission. (2009) Diario de Neurociencia. 29(19):6207-16. DOI: 10.1523/JNEUROSCI.3701-08.2009

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Nassi, J.J., Callaway, E.M. Parallel processing strategies of the primate visual system. (2009) Nature Reviews Neuroscience. 10(5):360-372. DOI: 10.1038/nrn2619

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Nimmerjahn, A., Mukamel, E.A., Schnitzer, M.J. Motor behavior activates Bergmann glial networks. (2009) Neurona. 62(3):400-12. DOI: 10.1016/j.neuron.2009.03.019

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Suh, H., Deng, W., Gage, F.H. Signaling in adult neurogenesis. (2009) Annual Review of Cell and Developmental Biology. 25:253-75. DOI: 10.1146/annurev.cellbio.042308.113256

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Stiefel, K.M., Gutkin, B.S., Sejnowski, T.J. The effects of cholinergic neuromodulation on neuronal phase-response curves of modeled cortical neurons. (2009) Journal of Computational Neuroscience. 26(2):289-301. DOI: 10.1007/s10827-008-0111-9

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Sharpee, T.O., Victor, J.D. Contextual modulation of V1 receptive fields depends on their spatial symmetry. (2009) Journal of Computational Neuroscience. 26(2):203-18. DOI: 10.1007/s10827-008-0107-5

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Nimmerjahn, A. Astrocytes going live: advances and challenges. (2009) Journal of Physiology. 587(Pt 8):1639-47. DOI: 10.1113/jphysiol.2008.167171

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Schwartz, O., Sejnowski, T.J., Dayan, P. Perceptual organization in the tilt illusion. (2009) Journal of Vision. 9(4):19.1-20. DOI: 10.1167/9.4.19

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Andrews, M.R., Czvitkovich, S., Dassie, E., Vogelaar, C.F., Faissner, A., Blits, B., Gage, F.H., ffrench-Constant, C., Fawcett, J.W. Alpha9 integrin promotes neurite outgrowth on tenascin-C and enhances sensory axon regeneration. (2009) Diario de Neurociencia. 29(17):5546-57. DOI: 10.1523/JNEUROSCI.0759-09.2009

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Saijo, K., Winner, B., Carson, C.T., Collier, J.G., Boyer, L., Rosenfeld, M.G., Gage, F.H., Glass, C.K. A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death. (2009) Móvil. 137(1):47-59. DOI: 10.1016/j.cell.2009.01.038

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Denli, A.M., Cao, X., Gage, F.H. miR-9 and TLX: chasing tails in neural stem cells. (2009) Nature Structural & Molecular Biology. 16(4):346-7. DOI: 10.1038/nsmb0409-346

Zhao, C., Suh, H., Gage, F.H. Notch keeps ependymal cells in line. (2009) Nature Neuroscience. 12(3):243-5. DOI: 10.1038/nn0309-243

Bazhenov, M., Timofeev, I., Fröhlich, F., Sejnowski, T.J. Cellular and network mechanisms of electrographic seizures. (2009) Drug Discov Today Dis Models. 5(1):45-57. DOI: 10.1016/j.ddmod.2008.07.005

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Jessberger, S., Clark, R.E., Broadbent, N.J., Clemenson, G.D., Consiglio, A., Lie, D.C., Squire, L.R., Gage, F.H. Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats. (2009) Learn. Mem. 16(2):147-54. DOI: 10.1101/lm.1172609

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Yeo, G.W., Coufal, N.G., Liang, T.Y., Peng, G.E., Fu, X.D., Gage, F.H. An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells. (2009) Nature Structural & Molecular Biology. 16(2):130-7. DOI: 10.1038/nsmb.1545

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Jessberger, S., Gage, F.H. Fate plasticity of adult hippocampal progenitors: biological relevance and therapeutic use. (2009) Tendencias en Ciencias Farmacológicas. 30(2):61-5. DOI: 10.1016/j.tips.2008.11.003

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Kouh, M., Sharpee, T.O. Estimating linear-nonlinear models using Renyi divergences. (2009) Network. 20(2):49-68. DOI: 10.1080/09548980902950891

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Aimone, J.B., Wiles, J., Gage, F.H. Computational influence of adult neurogenesis on memory encoding. (2009) Neurona. 61(2):187-202. DOI: 10.1016/j.neuron.2008.11.026

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Xu, X., Callaway, E.M. Laminar specificity of functional input to distinct types of inhibitory cortical neurons. (2009) Diario de Neurociencia. 29(1):70-85. DOI: 10.1523/JNEUROSCI.4104-08.2009

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Marumoto, T., Tashiro, A., Friedmann-Morvinski, D., Scadeng, M., Soda, Y., Gage, F.H., Verma, I.M. Development of a novel mouse glioma model using lentiviral vectors. (2009) Medicina Natural. 15(1):110-6. DOI: 10.1038/nm.1863

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Hao, J., Attias, H., Nagarajan, S., Lee, T.W., Sejnowski, T.J. Speech Enhancement, Gain, and Noise Spectrum Adaptation Using Approximate Bayesian Estimation. (2009) IEEE Trans Audio Speech Lang Process. 17(1):24-37. DOI: 10.1109/TASL.2008.2005342

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Rose, J.B., Crews, L., Rockenstein, E., Adame, A., Mante, M., Hersh, L.B., Gage, F.H., Spencer, B., Potkar, R., Marr, R.A., Masliah, E. Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease. (2009) Diario de Neurociencia. 29(4):1115-25. DOI: 10.1523/JNEUROSCI.4220-08.2009

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Callaway, E.M. Transneuronal circuit tracing with neurotropic viruses. (2008) Current Opinion in Neurobiology. 18(6):617-623. DOI: 10.1016/j.conb.2009.03.007

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Stiefel, K.M., Gutkin, B.S., Sejnowski, T.J. Cholinergic neuromodulation changes phase response curve shape and type in cortical pyramidal neurons. (2008) PLOS One. 3(12):e3947. DOI: 10.1371/journal.pone.0003947

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Prescott, S.A., Sejnowski, T.J. Spike-rate coding and spike-time coding are affected oppositely by different adaptation mechanisms. (2008) Diario de Neurociencia. 28(50):13649-13661. DOI: 10.1523/JNEUROSCI.1792-08.2008

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Marchetto, M.C., Muotri, A.R., Mu, Y., Smith, A.M., Cezar, G.G., Gage, F.H. Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor neurons derived from human embryonic stem cells. (2008) Células Madre. 3(6):649-57. DOI: 10.1016/j.stem.2008.10.001

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Cao, X., Pfaff, S.L., Gage, F.H. YAP regulates neural progenitor cell number via the TEA domain transcription factor. (2008) Genes y Desarrollo. 22(23):3320-34. DOI: 10.1101/gad.1726608

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Prescott, S.A., Ratté, S., De Koninck, Y., Sejnowski, T.J. Pyramidal neurons switch from integrators in vitro to resonators under in vivo-like conditions. (2008) Journal of Neurophysiology. 100(6):3030-42. DOI: 10.1152/jn.90634.2008

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Jessberger, S., Gage, F.H. Stem-cell-associated structural and functional plasticity in the aging hippocampus. (2008) Psychol Aging. 23(4):684-91. DOI: 10.1037/a0014188

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Thompson, C.L., Pathak, S.D., Jeromin, A., Ng, L.L., MacPherson, C.R., Mortrud, M.T., Cusick, A., Riley, Z.L., Sunkin, S.M., Bernard, A., Puchalski, R.B., Gage, F.H., Jones, A.R., Bajic, V.B., Hawrylycz, M.J., Lein, E.S. Genomic anatomy of the hippocampus. (2008) Neurona. 60(6):1010-21. DOI: 10.1016/j.neuron.2008.12.008

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Jessberger, S., Aigner, S., Clemenson, G.D., Toni, N., Lie, D.C., Karalay, O., Overall, R., Kempermann, G., Gage, F.H. Cdk5 regulates accurate maturation of newborn granule cells in the adult hippocampus. (2008) PLOS Biología. 6(11):e272. DOI: 10.1371/journal.pbio.0060272

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Flusberg, B.A., Nimmerjahn, A., Cocker, E.D., Mukamel, E.A., Barretto, R.P., Ko, T.H., Burns, L.D., Jung, J.C., Schnitzer, M.J. High-speed, miniaturized fluorescence microscopy in freely moving mice. (2008) Nature Methods. 5(11):935-8. DOI: 10.1038/nmeth.1256

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Spencer, B., Marr, R.A., Rockenstein, E., Crews, L., Adame, A., Potkar, R., Patrick, C., Gage, F.H., Verma, I.M., Masliah, E. Long-term neprilysin gene transfer is associated with reduced levels of intracellular Abeta and behavioral improvement in APP transgenic mice. (2008) BMC Neurosci. 9:109. DOI: 10.1186/1471-2202-9-109

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Kohyama, J., Kojima, T., Takatsuka, E., Yamashita, T., Namiki, J., Hsieh, J., Gage, F.H., Namihira, M., Okano, H., Sawamoto, K., Nakashima, K. Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. (2008) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 105(46):18012-7. DOI: 10.1073/pnas.0808417105

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Fröhlich, F., Bazhenov, M., Iragui-Madoz, V., Sejnowski, T.J. Potassium dynamics in the epileptic cortex: new insights on an old topic. (2008) The Neuroscientist. 14(5):422-433. DOI: 10.1177/1073858408317955

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Zhang, Y., Narayan, S., Geiman, E., Lanuza, G.M., Velasquez, T., Shanks, B., Akay, T., Dyck, J., Pearson, K., Gosgnach, S., Fan, C.M., Goulding, M. V3 spinal neurons establish a robust and balanced locomotor rhythm during walking. (2008) Neurona. 60(1):84-96. DOI: 10.1016/j.neuron.2008.09.027

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Kerr, R.A., Bartol, T.M., Kaminsky, B., Dittrich, M., Chang, J.C., Baden, S.B., Sejnowski, T.J., Stiles, J.R. FAST MONTE CARLO SIMULATION METHODS FOR BIOLOGICAL REACTION-DIFFUSION SYSTEMS IN SOLUTION AND ON SURFACES. (2008) SIAM Journal on Scientific Computing. 30(6):3126. DOI: 10.1137/070692017

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Prescott, S.A., De Koninck, Y., Sejnowski, T.J. Biophysical basis for three distinct dynamical mechanisms of action potential initiation. (2008) PLOS Biología Computacional. 4(10):e1000198. DOI: 10.1371/journal.pcbi.1000198

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Transduction of graded synaptic input into trains of all-or-none action potentials (spikes) is a crucial step in neural coding. Hodgkin identified three classes of neurons with qualitatively different analog-to-digital transduction properties. Despite widespread use of this classification scheme, a generalizable explanation of its biophysical basis has not been described. We recorded from spinal sensory neurons representing each class and reproduced their transduction properties in a minimal model. With phase plane and bifurcation analysis, each class of excitability was shown to derive from distinct spike initiating dynamics. Excitability could be converted between all three classes by varying single parameters; moreover, several parameters, when varied one at a time, had functionally equivalent effects on excitability. From this, we conclude that the spike-initiating dynamics associated with each of Hodgkin's classes represent different outcomes in a nonlinear competition between oppositely directed, kinetically mismatched currents. Class 1 excitability occurs through a saddle node on invariant circle bifurcation when net current at perithreshold potentials is inward (depolarizing) at steady state. Class 2 excitability occurs through a Hopf bifurcation when, despite net current being outward (hyperpolarizing) at steady state, spike initiation occurs because inward current activates faster than outward current. Class 3 excitability occurs through a quasi-separatrix crossing when fast-activating inward current overpowers slow-activating outward current during a stimulus transient, although slow-activating outward current dominates during constant stimulation. Experiments confirmed that different classes of spinal lamina I neurons express the subthreshold currents predicted by our simulations and, further, that those currents are necessary for the excitability in each cell class. Thus, our results demonstrate that all three classes of excitability arise from a continuum in the direction and magnitude of subthreshold currents. Through detailed analysis of the spike-initiating process, we have explained a fundamental link between biophysical properties and qualitative differences in how neurons encode sensory input.

Barbour, D.L., Callaway, E.M. Excitatory local connections of superficial neurons in rat auditory cortex. (2008) Diario de Neurociencia. 28(44):11174-11185. DOI: 10.1523/JNEUROSCI.2093-08.2008

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Pagliardini, S., Ren, J., Gray, P.A., Vandunk, C., Gross, M., Goulding, M., Greer, J.J. Central respiratory rhythmogenesis is abnormal in lbx1- deficient mice. (2008) Diario de Neurociencia. 28(43):11030-41. DOI: 10.1523/JNEUROSCI.1648-08.2008

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Li, X., Barkho, B.Z., Luo, Y., Smrt, R.D., Santistevan, N.J., Liu, C., Kuwabara, T., Gage, F.H., Zhao, X. Epigenetic regulation of the stem cell mitogen Fgf-2 by Mbd1 in adult neural stem/progenitor cells. (2008) Revista de Química Biológica. 283(41):27644-52. DOI: 10.1074/jbc.M804899200

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Pfaff, S.L. Developmental neuroscience: Hox and Fox. (2008) Naturaleza. 455(7211):295-7. DOI: 10.1038/455295a

Lopreore, C.L., Bartol, T.M., Coggan, J.S., Keller, D.X., Sosinsky, G.E., Ellisman, M.H., Sejnowski, T.J. Computational modeling of three-dimensional electrodiffusion in biological systems: application to the node of Ranvier. (2008) Biophysical Journal. 95(6):2624-2635. DOI: 10.1529/biophysj.108.132167

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Gage, F.H., Selzer, M.E. Experts provide insights into emerging field of brain and spine repair. Interview by Bridget M. Kuehn. (2008) JAMA. 300(9):1013-4. DOI: 10.1001/jama.300.9.1013

Toni, N., Laplagne, D.A., Zhao, C., Lombardi, G., Ribak, C.E., Gage, F.H., Schinder, A.F. Neurons born in the adult dentate gyrus form functional synapses with target cells. (2008) Nature Neuroscience. 11(8):901-7. DOI: 10.1038/nn.2156

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Jessberger, S., Toni, N., Clemenson, G.D., Ray, J., Gage, F.H. Directed differentiation of hippocampal stem/progenitor cells in the adult brain. (2008) Nature Neuroscience. 11(8):888-93. DOI: 10.1038/nn.2148

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Low, P.S., Shank, S.S., Sejnowski, T.J., Margoliash, D. Mammalian-like features of sleep structure in zebra finches. (2008) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 105(26):9081-9086. DOI: 10.1073/pnas.0703452105

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Wang, Z., Li, L., Goulding, M., Frank, E. Early postnatal development of reciprocal Ia inhibition in the murine spinal cord. (2008) Journal of Neurophysiology. 100(1):185-96. DOI: 10.1152/jn.90354.2008

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Atencio, C.A., Sharpee, T.O., Schreiner, C.E. Cooperative nonlinearities in auditory cortical neurons. (2008) Neurona. 58(6):956-66. DOI: 10.1016/j.neuron.2008.04.026

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Lee, S., Lee, B., Joshi, K., Pfaff, S.L., Lee, J.W., Lee, S.K. A regulatory network to segregate the identity of neuronal subtypes. (2008) Developmental Cell. 14(6):877-89. DOI: 10.1016/j.devcel.2008.03.021

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Sharpee, T.O., Miller, K.D., Stryker, M.P. On the importance of static nonlinearity in estimating spatiotemporal neural filters with natural stimuli. (2008) Journal of Neurophysiology. 99(5):2496-509. DOI: 10.1152/jn.01397.2007

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Tan, W., Janczewski, W.A., Yang, P., Shao, X.M., Callaway, E.M., Feldman, J.L. Silencing preBötzinger complex somatostatin-expressing neurons induces persistent apnea in awake rat. (2008) Nature Neuroscience. 11(5):538-540. DOI: 10.1038/nn.2104

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Yeo, G.W., Coufal, N., Aigner, S., Winner, B., Scolnick, J.A., Marchetto, M.C., Muotri, A.R., Carson, C., Gage, F.H. Multiple layers of molecular controls modulate self-renewal and neuronal lineage specification of embryonic stem cells. (2008) Genética Molecular Humana. 17(R1):R67-75. DOI: 10.1093/hmg/ddn065

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Keller, D.X., Franks, K.M., Bartol, T.M., Sejnowski, T.J. Calmodulin activation by calcium transients in the postsynaptic density of dendritic spines. (2008) PLOS One. 3(4):e2045. DOI: 10.1371/journal.pone.0002045

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Finelli, L.A., Haney, S., Bazhenov, M., Stopfer, M., Sejnowski, T.J. Synaptic learning rules and sparse coding in a model sensory system. (2008) PLOS Biología Computacional. 4(4):e1000062. DOI: 10.1371/journal.pcbi.1000062

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Gallarda, B.W., Bonanomi, D., Müller, D., Brown, A., Alaynick, W.A., Andrews, S.E., Lemke, G., Pfaff, S.L., Marquardt, T. Segregation of axial motor and sensory pathways via heterotypic trans-axonal signaling. (2008) Ciencia. 320(5873):233-6. DOI: 10.1126/science.1153758

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Xu, Y., Lopes, C., Qian, Y., Liu, Y., Cheng, L., Goulding, M., Turner, E.E., Lima, D., Ma, Q. Tlx1 and Tlx3 coordinate specification of dorsal horn pain-modulatory peptidergic neurons. (2008) Diario de Neurociencia. 28(15):4037-46. DOI: 10.1523/JNEUROSCI.4126-07.2008

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Ma, Y.C., Song, M.R., Park, J.P., Henry Ho, H.Y., Hu, L., Kurtev, M.V., Zieg, J., Ma, Q., Pfaff, S.L., Greenberg, M.E. Regulation of motor neuron specification by phosphorylation of neurogenin 2. (2008) Neurona. 58(1):65-77. DOI: 10.1016/j.neuron.2008.01.037

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Larsen, D.D., Wickersham, I.R., Callaway, E.M. Retrograde tracing with recombinant rabies virus reveals correlations between projection targets and dendritic architecture in layer 5 of mouse barrel cortex. (2008) Front Neural Circuits. 1:5. DOI: 10.3389/neuro.04.005.2007

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Luo, L., Callaway, E.M., Svoboda, K. Genetic dissection of neural circuits. (2008) Neurona. 57(5):634-660. DOI: 10.1016/j.neuron.2008.01.002

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Zhao, C., Deng, W., Gage, F.H. Mechanisms and functional implications of adult neurogenesis. (2008) Móvil. 132(4):645-60. DOI: 10.1016/j.cell.2008.01.033

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Fröhlich, F., Bazhenov, M., Sejnowski, T.J. Pathological effect of homeostatic synaptic scaling on network dynamics in diseases of the cortex. (2008) Diario de Neurociencia. 28(7):1709-1720. DOI: 10.1523/JNEUROSCI.4263-07.2008

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Zhang, C.L., Zou, Y., He, W., Gage, F.H., Evans, R.M. A role for adult TLX-positive neural stem cells in learning and behaviour. (2008) Naturaleza. 451(7181):1004-7. DOI: 10.1038/nature06562

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Chen, E., Stiefel, K.M., Sejnowski, T.J., Bullock, T.H. Model of traveling waves in a coral nerve network. (2008) J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 194(2):195-200. DOI: 10.1007/s00359-007-0305-z

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Tiesinga, P., Fellous, J.M., Sejnowski, T.J. Regulation of spike timing in visual cortical circuits. (2008) Nature Reviews Neuroscience. 9(2):97-107. DOI: 10.1038/nrn2315

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Ghosh, S., Marquardt, T., Thaler, J.P., Carter, N., Andrews, S.E., Pfaff, S.L., Hunter, T. Instructive role of aPKCzeta subcellular localization in the assembly of adherens junctions in neural progenitors. (2008) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 105(1):335-40. DOI: 10.1073/pnas.0705713105

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Nielsen, K.J., Callaway, E.M. More than a feeling: sensation from cortical stimulation. (2008) Nature Neuroscience. 11(1):10-11. DOI: 10.1038/nn0108-10

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Englitz, B., Stiefel, K.M., Sejnowski, T.J. Irregular firing of isolated cortical interneurons in vitro driven by intrinsic stochastic mechanisms. (2008) Computación neuronal. 20(1):44-64. DOI: 10.1162/neco.2008.20.1.44

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Cezar, G.G., Quam, J.A., Smith, A.M., Rosa, G.J., Piekarczyk, M.S., Brown, J.F., Gage, F.H., Muotri, A.R. Identification of small molecules from human embryonic stem cells using metabolomics. (2007) Stem Cells Dev. 16(6):869-82. DOI: 10.1089/scd.2007.0022

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Yao, W., Nathanson, J., Lian, I., Gage, F.H., Sung, L.A. Mouse erythrocyte tropomodulin in the brain reported by lacZ knocked-in downstream from the E1 promoter. (2007) Gene Expr. Patterns. 8(1):36-46. DOI: 10.1016/j.modgep.2007.08.002

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Suh, H., Consiglio, A., Ray, J., Sawai, T., D'Amour, K.A., Gage, F.H. In vivo fate analysis reveals the multipotent and self-renewal capacities of Sox2+ neural stem cells in the adult hippocampus. (2007) Células Madre. 1(5):515-28. DOI: 10.1016/j.stem.2007.09.002

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Lewcock, J.W., Genoud, N., Lettieri, K., Pfaff, S.L. The ubiquitin ligase Phr1 regulates axon outgrowth through modulation of microtubule dynamics. (2007) Neurona. 56(4):604-20. DOI: 10.1016/j.neuron.2007.09.009

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Clifford, C.W., Webster, M.A., Stanley, G.B., Stocker, A.A., Kohn, A., Sharpee, T.O., Schwartz, O. Visual adaptation: neural, psychological and computational aspects. (2007) Vision Research. 47(25):3125-31. DOI: 10.1016/j.visres.2007.08.023

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Yeo, G.W., Xu, X., Liang, T.Y., Muotri, A.R., Carson, C.T., Coufal, N.G., Gage, F.H. Alternative splicing events identified in human embryonic stem cells and neural progenitors. (2007) PLOS Biología Computacional. 3(10):1951-67. DOI: 10.1371/journal.pcbi.0030196

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Human embryonic stem cells (hESCs) and neural progenitor (NP) cells are excellent models for recapitulating early neuronal development in vitro, and are key to establishing strategies for the treatment of degenerative disorders. While much effort had been undertaken to analyze transcriptional and epigenetic differences during the transition of hESC to NP, very little work has been performed to understand post-transcriptional changes during neuronal differentiation. Alternative RNA splicing (AS), a major form of post-transcriptional gene regulation, is important in mammalian development and neuronal function. Human ESC, hESC-derived NP, and human central nervous system stem cells were compared using Affymetrix exon arrays. We introduced an outlier detection approach, REAP (Regression-based Exon Array Protocol), to identify 1,737 internal exons that are predicted to undergo AS in NP compared to hESC. Experimental validation of REAP-predicted AS events indicated a threshold-dependent sensitivity ranging from 56% to 69%, at a specificity of 77% to 96%. REAP predictions significantly overlapped sets of alternative events identified using expressed sequence tags and evolutionarily conserved AS events. Our results also reveal that focusing on differentially expressed genes between hESC and NP will overlook 14% of potential AS genes. In addition, we found that REAP predictions are enriched in genes encoding serine/threonine kinase and helicase activities. An example is a REAP-predicted alternative exon in the SLK (serine/threonine kinase 2) gene that is differentially included in hESC, but skipped in NP as well as in other differentiated tissues. Lastly, comparative sequence analysis revealed conserved intronic cis-regulatory elements such as the FOX1/2 binding site GCAUG as being proximal to candidate AS exons, suggesting that FOX1/2 may participate in the regulation of AS in NP and hESC. In summary, a new methodology for exon array analysis was introduced, leading to new insights into the complexity of AS in human embryonic stem cells and their transition to neural stem cells.

Muotri, A.R., Marchetto, M.C., Coufal, N.G., Gage, F.H. The necessary junk: new functions for transposable elements. (2007) Genética Molecular Humana. 16 Spec No:R159-67. DOI: 10.1093/hmg/ddm196

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Nassi, J.J., Callaway, E.M. Specialized circuits from primary visual cortex to V2 and area MT. (2007) Neurona. 55(5):799-808. DOI: 10.1016/j.neuron.2007.07.037

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Sharpee, T.O. Comparison of information and variance maximization strategies for characterizing neural feature selectivity. (2007) Stat Med. 26(21):4009-31. DOI: 10.1002/sim.2931

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Jessberger, S., Zhao, C., Toni, N., Clemenson, G.D., Li, Y., Gage, F.H. Seizure-associated, aberrant neurogenesis in adult rats characterized with retrovirus-mediated cell labeling. (2007) Diario de Neurociencia. 27(35):9400-7. DOI: 10.1523/JNEUROSCI.2002-07.2007

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Vucinić, D., Sejnowski, T.J. A compact multiphoton 3D imaging system for recording fast neuronal activity. (2007) PLOS One. 2(8):e699. DOI: 10.1371/journal.pone.0000699

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Lanctot, P.M., Gage, F.H., Varki, A.P. The glycans of stem cells. (2007) Current Opinion in Chemical Biology. 11(4):373-80. DOI: 10.1016/j.cbpa.2007.05.032

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Sharpee, T., Bialek, W. Neural decision boundaries for maximal information transmission. (2007) PLOS One. 2(7):e646. DOI: 10.1371/journal.pone.0000646

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Aigner, S., Denli, A.M., Gage, F.H. A novel model for an older remodeler: the BAF swap in neurogenesis. (2007) Neurona. 55(2):171-3. DOI: 10.1016/j.neuron.2007.07.004

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Tour, O., Adams, S.R., Kerr, R.A., Meijer, R.M., Sejnowski, T.J., Tsien, R.W., Tsien, R.Y. Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. (2007) Nature Chemical Biology. 3(7):423-31. DOI: 10.1038/nchembio.2007.4

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Stiefel, K.M., Sejnowski, T.J. Mapping function onto neuronal morphology. (2007) Journal of Neurophysiology. 98(1):513-26. DOI: 10.1152/jn.00865.2006

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Smrt, R.D., Eaves-Egenes, J., Barkho, B.Z., Santistevan, N.J., Zhao, C., Aimone, J.B., Gage, F.H., Zhao, X. Mecp2 deficiency leads to delayed maturation and altered gene expression in hippocampal neurons. (2007) Neurobiol. Dis. 27(1):77-89. DOI: 10.1016/j.nbd.2007.04.005

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Garcia-Perez, J.L., Marchetto, M.C., Muotri, A.R., Coufal, N.G., Gage, F.H., O'Shea, K.S., Moran, J.V. LINE-1 retrotransposition in human embryonic stem cells. (2007) Genética Molecular Humana. 16(13):1569-77. DOI: 10.1093/hmg/ddm105

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Toni, N., Teng, E.M., Bushong, E.A., Aimone, J.B., Zhao, C., Consiglio, A., van Praag, H., Martone, M.E., Ellisman, M.H., Gage, F.H. Synapse formation on neurons born in the adult hippocampus. (2007) Nature Neuroscience. 10(6):727-34. DOI: 10.1038/nn1908

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El-Amouri, S.S., Zhu, H., Yu, J., Gage, F.H., Verma, I.M., Kindy, M.S. Neprilysin protects neurons against Abeta peptide toxicity. (2007) Brain Research. 1152:191-200. DOI: 10.1016/j.brainres.2007.03.072

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Wachtler, T., Doi, E., Lee, T., Sejnowski, T.J. Cone selectivity derived from the responses of the retinal cone mosaic to natural scenes. (2007) Journal of Vision. 7(8):6. DOI: 10.1167/7.8.6

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Parent, J.M., Jessberger, S., Gage, F.H., Gong, C. Is neurogenesis reparative after status epilepticus? (2007) Epilepsia. 48 Suppl 8:69-71.

Jessberger, S., Nakashima, K., Clemenson, G.D., Mejia, E., Mathews, E., Ure, K., Ogawa, S., Sinton, C.M., Gage, F.H., Hsieh, J. Epigenetic modulation of seizure-induced neurogenesis and cognitive decline. (2007) Diario de Neurociencia. 27(22):5967-75. DOI: 10.1523/JNEUROSCI.0110-07.2007

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van Praag, H., Lucero, M.J., Yeo, G.W., Stecker, K., Heivand, N., Zhao, C., Yip, E., Afanador, M., Schroeter, H., Hammerstone, J., Gage, F.H. Plant-derived flavanol (-)epicatechin enhances angiogenesis and retention of spatial memory in mice. (2007) Diario de Neurociencia. 27(22):5869-78. DOI: 10.1523/JNEUROSCI.0914-07.2007

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Laplagne, D.A., Kamienkowski, J.E., Espósito, M.S., Piatti, V.C., Zhao, C., Gage, F.H., Schinder, A.F. Similar GABAergic inputs in dentate granule cells born during embryonic and adult neurogenesis. (2007) European Journal of Neuroscience. 25(10):2973-81. DOI: 10.1111/j.1460-9568.2007.05549.x

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Jessberger, S., Clemenson, G.D., Gage, F.H. Spontaneous fusion and nonclonal growth of adult neural stem cells. (2007) Células madre. 25(4):871-4. DOI: 10.1634/stemcells.2006-0620

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Fox, M.A., Sanes, J.R., Borza, D.B., Eswarakumar, V.P., Fässler, R., Hudson, B.G., John, S.W., Ninomiya, Y., Pedchenko, V., Pfaff, S.L., Rheault, M.N., Sado, Y., Segal, Y., Werle, M.J., Umemori, H. Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals. (2007) Móvil. 129(1):179-93. DOI: 10.1016/j.cell.2007.02.035

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Mishra, J., Martinez, A., Sejnowski, T.J., Hillyard, S.A. Early cross-modal interactions in auditory and visual cortex underlie a sound-induced visual illusion. (2007) Diario de Neurociencia. 27(15):4120-4131. DOI: 10.1523/JNEUROSCI.4912-06.2007

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Tashiro, A., Makino, H., Gage, F.H. Experience-specific functional modification of the dentate gyrus through adult neurogenesis: a critical period during an immature stage. (2007) Diario de Neurociencia. 27(12):3252-9. DOI: 10.1523/JNEUROSCI.4941-06.2007

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Leingärtner, A., Thuret, S., Kroll, T.T., Chou, S.J., Leasure, J.L., Gage, F.H., O'Leary, D.D. Cortical area size dictates performance at modality-specific behaviors. (2007) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 104(10):4153-8. DOI: 10.1073/pnas.0611723104

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Cao, X., Pfaff, S.L., Gage, F.H. A functional study of miR-124 in the developing neural tube. (2007) Genes y Desarrollo. 21(5):531-6. DOI: 10.1101/gad.1519207

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Wickersham, I.R., Lyon, D.C., Barnard, R.J., Mori, T., Finke, S., Conzelmann, K.K., Young, J.A., Callaway, E.M. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. (2007) Neurona. 53(5):639-647. DOI: 10.1016/j.neuron.2007.01.033

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Peng, C.Y., Yajima, H., Burns, C.E., Zon, L.I., Sisodia, S.S., Pfaff, S.L., Sharma, K. Notch and MAML signaling drives Scl-dependent interneuron diversity in the spinal cord. (2007) Neurona. 53(6):813-27. DOI: 10.1016/j.neuron.2007.02.019

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Eagleman, D.M., Sejnowski, T.J. Motion signals bias localization judgments: a unified explanation for the flash-lag, flash-drag, flash-jump, and Frohlich illusions. (2007) Journal of Vision. 7(4):3. DOI: 10.1167/7.4.3

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Pereira, A.C., Huddleston, D.E., Brickman, A.M., Sosunov, A.A., Hen, R., McKhann, G.M., Sloan, R., Gage, F.H., Brown, T.R., Small, S.A. An in vivo correlate of exercise-induced neurogenesis in the adult dentate gyrus. (2007) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 104(13):5638-43. DOI: 10.1073/pnas.0611721104

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Delorme, A., Sejnowski, T., Makeig, S. Enhanced detection of artifacts in EEG data using higher-order statistics and independent component analysis. (2007) Neuroimage. 34(4):1443-1449. DOI: 10.1016/j.neuroimage.2006.11.004

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Odani, N., Pfaff, S.L., Nakamura, H., Funahashi, J. Cloning and developmental expression of a chick G-protein-coupled receptor SCGPR1. (2007) Gene Expression Patterns. 7(4):375-80. DOI: 10.1016/j.modgep.2006.12.003

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Siegel, R.M., Duann, J.R., Jung, T.P., Sejnowski, T. Spatiotemporal dynamics of the functional architecture for gain fields in inferior parietal lobule of behaving monkey. (2007) Cerebral Cortex . 17(2):378-390. DOI: 10.1093/cercor/bhj155

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Suzuki, S., Gerhold, L.M., Böttner, M., Rau, S.W., Dela Cruz, C., Yang, E., Zhu, H., Yu, J., Cashion, A.B., Kindy, M.S., Merchenthaler, I., Gage, F.H., Wise, P.M. Estradiol enhances neurogenesis following ischemic stroke through estrogen receptors alpha and beta. (2007) Journal of Comparative Neurology. 500(6):1064-75. DOI: 10.1002/cne.21240

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Wickersham, I.R., Finke, S., Conzelmann, K.K., Callaway, E.M. Retrograde neuronal tracing with a deletion-mutant rabies virus. (2007) Nature Methods. 4(1):47-49. DOI: 10.1038/nmeth999

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Pillai, A., Mansouri, A., Behringer, R., Westphal, H., Goulding, M. Lhx1 and Lhx5 maintain the inhibitory-neurotransmitter status of interneurons in the dorsal spinal cord. (2007) Development. 134(2):357-66. DOI: 10.1242/dev.02717

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Jessberger, S., Gage, F.H. ZOOMING IN: a new high-resolution gene expression atlas of the brain. (2007) Mol. Syst. Biol. 3:75. DOI: 10.1038/msb4100122

Zhang, Y., Kim, Y., Genoud, N., Gao, J., Kelly, J.W., Pfaff, S.L., Gill, G.N., Dixon, J.E., Noel, J.P. Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1. (2006) Biología Celular Molecular. 24(5):759-70. DOI: 10.1016/j.molcel.2006.10.027

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Nassi, J.J., Callaway, E.M. Multiple circuits relaying primate parallel visual pathways to the middle temporal area. (2006) Diario de Neurociencia. 26(49):12789-12798. DOI: 10.1523/JNEUROSCI.4044-06.2006

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Song, M.R., Shirasaki, R., Cai, C.L., Ruiz, E.C., Evans, S.M., Lee, S.K., Pfaff, S.L. T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. (2006) Development. 133(24):4945-55. DOI: 10.1242/dev.02694

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Sceniak, M.P., Chatterjee, S., Callaway, E.M. Visual spatial summation in macaque geniculocortical afferents. (2006) Journal of Neurophysiology. 96(6):3474-3484. DOI: 10.1152/jn.00734.2006

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Miller, T.M., Kim, S.H., Yamanaka, K., Hester, M., Umapathi, P., Arnson, H., Rizo, L., Mendell, J.R., Gage, F.H., Cleveland, D.W., Kaspar, B.K. Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(51):19546-51. DOI: 10.1073/pnas.0609411103

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Suhonen, J., Ray, J., Blömer, U., Gage, F.H., Kaspar, B. Ex vivo and in vivo gene delivery to the brain. (2006) Current Protocols in Human Genetics. Chapter 13:Unit 13.3. DOI: 10.1002/0471142905.hg1303s51

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Schwartz, O., Sejnowski, T.J., Dayan, P. Soft mixer assignment in a hierarchical generative model of natural scene statistics. (2006) Computación neuronal. 18(11):2680-718. DOI: 10.1162/neco.2006.18.11.2680

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Xu, X., Roby, K.D., Callaway, E.M. Mouse cortical inhibitory neuron type that coexpresses somatostatin and calretinin. (2006) Journal of Comparative Neurology. 499(1):144-160. DOI: 10.1002/cne.21101

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Thallmair, M., Ray, J., Stallcup, W.B., Gage, F.H. Functional and morphological effects of NG2 proteoglycan deletion on hippocampal neurogenesis. (2006) Exp. Neurol. 202(1):167-78. DOI: 10.1016/j.expneurol.2006.05.025

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NG2-expressing cells are the largest proliferating cell population in the adult central nervous system. The function of NG2 proteoglycan or NG2-expressing cells in the adult brain, however, is unknown. So far, NG2-positive cells are thought to be mainly oligodendrocyte precursor cells. This view was recently challenged when NG2+/CNP-EGFP-positive cells were identified as multipotent progenitor cells in the postnatal and adult CNS (e.g., [Belachew, S., Chittajallu, R., Aguirre, A.A., Yuan, X., Kirby, M., Anderson, S., Gallo, V., 2003. Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent and generate functional neurons. J. Cell Biol. 161, 169-186]). In addition, purified NG2-expressing progenitor cells, were shown to differentiate into neurons and astrocytes in vitro [Sellers, D.L., Horner, P.J., 2005. Instructive niches: environmental instructions that confound NG2 proteoglycan expression and the fate-restriction of CNS progenitors J. Anat. 207, 727-734]. In this study, we focus on the influence of NG2 ablation on neurogenesis in the hippocampus, where putative multipotent NG2-positive cells reside, and on hippocampus-dependent behavior using NG2 knockout mice. Using the thymidine analogue bromodeoxyuridine (BrdU) to label dividing cells in vivo we show that the number of BrdU-positive cells was unchanged in the hippocampus of NG2 knockout mice 1 day after a series of BrdU injections. This finding suggests that the proliferation rate of hippocampal progenitor cells is not influenced by NG2. A few BrdU-positive cells were found in deeper layers of the granule zone 1 day after a series of BrdU injections, which is different from the wild type. The presence and the phenotype of newborn hippocampal cells were studied 4 weeks after a series of BrdU injections. The survival and differentiation of BrdU-positive cells in NG2 knockout hippocampus did not significantly differ from wild-type mice. Concurrently, the water maze task did not reveal obvious differences compared to wild-type animals. These results suggest that the null mutation for NG2 does not influence adult hippocampal neurogenesis or hippocampal-dependent behavioral tasks.

Borrell, V., Kaspar, B.K., Gage, F.H., Callaway, E.M. In vivo evidence for radial migration of neurons by long-distance somal translocation in the developing ferret visual cortex. (2006) Cereb. Cortex. 16(11):1571-83. DOI: 10.1093/cercor/bhj094

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Mishra, J., Fellous, J.M., Sejnowski, T.J. Selective attention through phase relationship of excitatory and inhibitory input synchrony in a model cortical neuron. (2006) Neural Networks. 19(9):1329-1346. DOI: 10.1016/j.neunet.2006.08.005

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Ramirez-Amaya, V., Marrone, D.F., Gage, F.H., Worley, P.F., Barnes, C.A. Integration of new neurons into functional neural networks. (2006) Diario de Neurociencia. 26(47):12237-41. DOI: 10.1523/JNEUROSCI.2195-06.2006

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Although it is established that new granule cells can be born and can survive in the adult mammalian hippocampus, there remains some question concerning the functional integration of these neurons into behaviorally relevant neural networks. By using high-resolution confocal microscopy, we have applied a new strategy to address the question of functional integration of newborn neurons into networks that mediate spatial information processing and memory formation. Exploration-induced expression of the immediate-early gene Arc in hippocampal cells has been linked to cellular activity observed in electrophysiological recordings under the same behavioral conditions. We investigated whether mature (5-month-old), newborn granule cells express Arc in response to a discrete spatial experience by detecting the expression of Arc in combination with NeuN (neuron-specific nuclear protein)-positive and bromodeoxyuridine-positive cells. We found that mature new granule cells do indeed express Arc in response to an exploration experience, supporting the idea that these cells are well integrated into hippocampal circuits. The proportion of mature newborn neurons that expressed Arc in response to exploration, however, was significantly higher (approximately 2.8%) than the proportion of cells that expressed Arc in the already existing population of granule cells (approximately 1.6%; p < 0.01). This finding extends previous data suggesting that the cellular physiology of newborn granule neurons differs from that of the existing population by indicating that these properties are retained in mature adult-generated neurons. Thus, these data have interesting implications for network models of spatial information processing and the role of hippocampal circuits in memory, indicating that mature new neurons are selectively recruited into hippocampal cell assemblies in higher proportions than older cells.

Laplagne, D.A., Espósito, M.S., Piatti, V.C., Morgenstern, N.A., Zhao, C., van Praag, H., Gage, F.H., Schinder, A.F. Functional convergence of neurons generated in the developing and adult hippocampus. (2006) PLOS Biología. 4(12):e409. DOI: 10.1371/journal.pbio.0040409

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Carson, C.T., Aigner, S., Gage, F.H. Stem cells: the good, bad and barely in control. (2006) Medicina Natural. 12(11):1237-8. DOI: 10.1038/nm1106-1237

Prescott, S.A., Sejnowski, T.J., De Koninck, Y. Reduction of anion reversal potential subverts the inhibitory control of firing rate in spinal lamina I neurons: towards a biophysical basis for neuropathic pain. (2006) Mol Pain. 2:32. DOI: 10.1186/1744-8069-2-32

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Horky, L.L., Galimi, F., Gage, F.H., Horner, P.J. Fate of endogenous stem/progenitor cells following spinal cord injury. (2006) Journal of Comparative Neurology. 498(4):525-38. DOI: 10.1002/cne.21065

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Callaway, E.M., Sanes, J.R. New technologies. (2006) Current Opinion in Neurobiology. 16(5):540-542. DOI: 10.1016/j.conb.2006.08.014

Prescott, S.A., Ratté, S., De Koninck, Y., Sejnowski, T.J. Nonlinear interaction between shunting and adaptation controls a switch between integration and coincidence detection in pyramidal neurons. (2006) Diario de Neurociencia. 26(36):9084-97. DOI: 10.1523/JNEUROSCI.1388-06.2006

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Jagasia, R., Song, H., Gage, F.H., Lie, D.C. New regulators in adult neurogenesis and their potential role for repair. (2006) Tendencias en Medicina Molecular. 12(9):400-5. DOI: 10.1016/j.molmed.2006.07.006

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Tashiro, A., Sandler, V.M., Toni, N., Zhao, C., Gage, F.H. NMDA-receptor-mediated, cell-specific integration of new neurons in adult dentate gyrus. (2006) Naturaleza. 442(7105):929-33. DOI: 10.1038/nature05028

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Vucinić, D., Bartol, T.M., Sejnowski, T.J. Hybrid reflecting objectives for functional multiphoton microscopy in turbid media. (2006) Opt Lett. 31(16):2447-2449.

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Kashani, A.H., Qiu, Z., Jurata, L., Lee, S.K., Pfaff, S., Goebbels, S., Nave, K.A., Ghosh, A. Calcium activation of the LMO4 transcription complex and its role in the patterning of thalamocortical connections. (2006) Diario de Neurociencia. 26(32):8398-408. DOI: 10.1523/JNEUROSCI.0618-06.2006

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Zhao, Y., Morales, D.C., Hermesz, E., Lee, W.K., Pfaff, S.L., Westphal, H. Reduced expression of the LIM-homeobox gene Lhx3 impairs growth and differentiation of Rathke's pouch and increases cell apoptosis during mouse pituitary development. (2006) Mechanisms of Development. 123(8):605-13. DOI: 10.1016/j.mod.2006.06.005

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Anemüller, J., Duann, J.R., Sejnowski, T.J., Makeig, S. Spatio-temporal dynamics in fMRI recordings revealed with complex independent component analysis. (2006) Neurocomputing. 69(13-15):1502-1512. DOI: 10.1016/j.neucom.2005.12.029

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Bhardwaj, R.D., Curtis, M.A., Spalding, K.L., Buchholz, B.A., Fink, D., Björk-Eriksson, T., Nordborg, C., Gage, F.H., Druid, H., Eriksson, P.S., Frisén, J. Neocortical neurogenesis in humans is restricted to development. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(33):12564-8. DOI: 10.1073/pnas.0605177103

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Thuret, S., Moon, L.D., Gage, F.H. Therapeutic interventions after spinal cord injury. (2006) Nature Reviews Neuroscience. 7(8):628-43. DOI: 10.1038/nrn1955

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Tan, E.M., Yamaguchi, Y., Horwitz, G.D., Gosgnach, S., Lein, E.S., Goulding, M., Albright, T.D., Callaway, E.M. Selective and quickly reversible inactivation of mammalian neurons in vivo using the Drosophila allatostatin receptor. (2006) Neurona. 51(2):157-70. DOI: 10.1016/j.neuron.2006.06.018

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Suzuki, A., Raya, A., Kawakami, Y., Morita, M., Matsui, T., Nakashima, K., Gage, F.H., Rodríguez-Esteban, C., Izpisúa Belmonte, J.C. Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(27):10294-9. DOI: 10.1073/pnas.0506945103

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Tashiro, A., Zhao, C., Gage, F.H. Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo. (2006) Nature Protocols. 1(6):3049-55. DOI: 10.1038/nprot.2006.473

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Muotri, A.R., Gage, F.H. Generation of neuronal variability and complexity. (2006) Naturaleza. 441(7097):1087-93. DOI: 10.1038/nature04959

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Cao, X., Yeo, G., Muotri, A.R., Kuwabara, T., Gage, F.H. Noncoding RNAs in the mammalian central nervous system. (2006) Revisión Anual de Neurociencia. 29:77-103. DOI: 10.1146/annurev.neuro.29.051605.112839

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Shirasaki, R., Lewcock, J.W., Lettieri, K., Pfaff, S.L. FGF as a target-derived chemoattractant for developing motor axons genetically programmed by the LIM code. (2006) Neurona. 50(6):841-53. DOI: 10.1016/j.neuron.2006.04.030

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Fröhlich, F., Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Slow state transitions of sustained neural oscillations by activity-dependent modulation of intrinsic excitability. (2006) Diario de Neurociencia. 26(23):6153-6162. DOI: 10.1523/JNEUROSCI.5509-05.2006

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Aimone, J.B., Wiles, J., Gage, F.H. Potential role for adult neurogenesis in the encoding of time in new memories. (2006) Nature Neuroscience. 9(6):723-7. DOI: 10.1038/nn1707

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Mizuguchi, R., Kriks, S., Cordes, R., Gossler, A., Ma, Q., Goulding, M. Ascl1 and Gsh1/2 control inhibitory and excitatory cell fate in spinal sensory interneurons. (2006) Nature Neuroscience. 9(6):770-8. DOI: 10.1038/nn1706

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Barkho, B.Z., Song, H., Aimone, J.B., Smrt, R.D., Kuwabara, T., Nakashima, K., Gage, F.H., Zhao, X. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. (2006) Stem Cells Dev. 15(3):407-21. DOI: 10.1089/scd.2006.15.407

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Zhang, C.L., Zou, Y., Yu, R.T., Gage, F.H., Evans, R.M. Nuclear receptor TLX prevents retinal dystrophy and recruits the corepressor atrophin1. (2006) Genes y Desarrollo. 20(10):1308-20. DOI: 10.1101/gad.1413606

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Nassi, J.J., Lyon, D.C., Callaway, E.M. The parvocellular LGN provides a robust disynaptic input to the visual motion area MT. (2006) Neurona. 50(2):319-327. DOI: 10.1016/j.neuron.2006.03.019

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Callaway, E.M. Should I stay or should I go? Presynaptic boutons in the adult cortex still haven't made up their minds. (2006) Neurona. 49(6):780-783. DOI: 10.1016/j.neuron.2006.03.007

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Gosgnach, S., Lanuza, G.M., Butt, S.J., Saueressig, H., Zhang, Y., Velasquez, T., Riethmacher, D., Callaway, E.M., Kiehn, O., Goulding, M. V1 spinal neurons regulate the speed of vertebrate locomotor outputs. (2006) Naturaleza. 440(7081):215-9. DOI: 10.1038/nature04545

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Jacobs, S., Lie, D.C., DeCicco, K.L., Shi, Y., DeLuca, L.M., Gage, F.H., Evans, R.M. Retinoic acid is required early during adult neurogenesis in the dentate gyrus. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(10):3902-7. DOI: 10.1073/pnas.0511294103

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Suzuki, A., Raya, A., Kawakami, Y., Morita, M., Matsui, T., Nakashima, K., Gage, F.H., Rodríguez-Esteban, C., Izpisúa Belmonte, J.C. Maintenance of embryonic stem cell pluripotency by Nanog-mediated reversal of mesoderm specification. (2006) Nature Clinical Practice. Cardiovascular Medicine. 3 Suppl 1:S114-22. DOI: 10.1038/ncpcardio0442

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Ray, J., Gage, F.H. Differential properties of adult rat and mouse brain-derived neural stem/progenitor cells. (2006) Molecular and Cellular Neurosciences. 31(3):560-73. DOI: 10.1016/j.mcn.2005.11.010

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Zarrinpar, A., Callaway, E.M. Local connections to specific types of layer 6 neurons in the rat visual cortex. (2006) Journal of Neurophysiology. 95(3):1751-1761. DOI: 10.1152/jn.00974.2005

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Nakahira, E., Kagawa, T., Shimizu, T., Goulding, M.D., Ikenaka, K. Direct evidence that ventral forebrain cells migrate to the cortex and contribute to the generation of cortical myelinating oligodendrocytes. (2006) Developmental Biology. 291(1):123-31. DOI: 10.1016/j.ydbio.2005.12.010

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Sejnowski, T.J., Paulsen, O. Network oscillations: emerging computational principles. (2006) Diario de Neurociencia. 26(6):1673-6. DOI: 10.1523/JNEUROSCI.3737-05d.2006

Sharpee, T.O., Sugihara, H., Kurgansky, A.V., Rebrik, S.P., Stryker, M.P., Miller, K.D. Adaptive filtering enhances information transmission in visual cortex. (2006) Naturaleza. 439(7079):936-42. DOI: 10.1038/nature04519

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Paulsen, O., Sejnowski, T.J. From invertebrate olfaction to human cognition: emerging computational functions of synchronized oscillatory activity. (2006) Diario de Neurociencia. 26(6):1661-1662. DOI: 10.1523/JNEUROSCI.3737-05a.2006

Yang, H., Lu, P., McKay, H.M., Bernot, T., Keirstead, H., Steward, O., Gage, F.H., Edgerton, V.R., Tuszynski, M.H. Endogenous neurogenesis replaces oligodendrocytes and astrocytes after primate spinal cord injury. (2006) Diario de Neurociencia. 26(8):2157-66. DOI: 10.1523/JNEUROSCI.4070-05.2005

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Zhao, C., Teng, E.M., Summers, R.G., Ming, G.L., Gage, F.H. Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus. (2006) Diario de Neurociencia. 26(1):3-11. DOI: 10.1523/JNEUROSCI.3648-05.2006

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Kerr, R.A., Levine, H., Sejnowski, T.J., Rappel, W.J. Division accuracy in a stochastic model of Min oscillations in Escherichia coli. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(2):347-352. DOI: 10.1073/pnas.0505825102

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Larsen, D.D., Callaway, E.M. Development of layer-specific axonal arborizations in mouse primary somatosensory cortex. (2006) Journal of Comparative Neurology. 494(3):398-414. DOI: 10.1002/cne.20754

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Victor, J.D., Mechler, F., Repucci, M.A., Purpura, K.P., Sharpee, T. Responses of V1 neurons to two-dimensional hermite functions. (2006) Journal of Neurophysiology. 95(1):379-400. DOI: 10.1152/jn.00498.2005

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Battista, D., Ferrari, C.C., Gage, F.H., Pitossi, F.J. Neurogenic niche modulation by activated microglia: transforming growth factor beta increases neurogenesis in the adult dentate gyrus. (2006) European Journal of Neuroscience. 23(1):83-93. DOI: 10.1111/j.1460-9568.2005.04539.x

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Alisch, R.S., Garcia-Perez, J.L., Muotri, A.R., Gage, F.H., Moran, J.V. Unconventional translation of mammalian LINE-1 retrotransposons. (2006) Genes y Desarrollo. 20(2):210-24. DOI: 10.1101/gad.1380406

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Kempermann, G., Chesler, E.J., Lu, L., Williams, R.W., Gage, F.H. Natural variation and genetic covariance in adult hippocampal neurogenesis. (2006) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 103(3):780-5. DOI: 10.1073/pnas.0510291103

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Warashina, M., Min, K.H., Kuwabara, T., Huynh, A., Gage, F.H., Schultz, P.G., Ding, S. A synthetic small molecule that induces neuronal differentiation of adult hippocampal neural progenitor cells. (2006) Angew. Chem. Int. Ed. Engl. 45(4):591-3. DOI: 10.1002/anie.200503089

Pizzo, D.P., Coufal, N.G., Lortie, M.J., Gage, F.H., Thal, L.J. Regulatable acetylcholine-producing fibroblasts enhance cognitive performance. (2006) Mol. Ther. 13(1):175-82. DOI: 10.1016/j.ymthe.2005.08.001

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Moon, L.D., Leasure, J.L., Gage, F.H., Bunge, M.B. Motor enrichment sustains hindlimb movement recovered after spinal cord injury and glial transplantation. (2006) Restorative Neurology and Neuroscience. 24(3):147-61.

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Muotri, A.R., Nakashima, K., Toni, N., Sandler, V.M., Gage, F.H. Development of functional human embryonic stem cell-derived neurons in mouse brain. (2005) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 102(51):18644-8. DOI: 10.1073/pnas.0509315102

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Quina, L.A., Pak, W., Lanier, J., Banwait, P., Gratwick, K., Liu, Y., Velasquez, T., O'Leary, D.D., Goulding, M., Turner, E.E. Brn3a-expressing retinal ganglion cells project specifically to thalamocortical and collicular visual pathways. (2005) Diario de Neurociencia. 25(50):11595-604. DOI: 10.1523/JNEUROSCI.2837-05.2005

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Alvarez, F.J., Jonas, P.C., Sapir, T., Hartley, R., Berrocal, M.C., Geiman, E.J., Todd, A.J., Goulding, M. Postnatal phenotype and localization of spinal cord V1 derived interneurons. (2005) Journal of Comparative Neurology. 493(2):177-92. DOI: 10.1002/cne.20711

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Hsieh, J., Gage, F.H. Chromatin remodeling in neural development and plasticity. (2005) Current Opinion in Cell Biology. 17(6):664-71. DOI: 10.1016/j.ceb.2005.09.002

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Song, M.R., Pfaff, S.L. Hox genes: the instructors working at motor pools. (2005) Móvil. 123(3):363-5. DOI: 10.1016/j.cell.2005.10.014

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Yoshimura, Y., Callaway, E.M. Fine-scale specificity of cortical networks depends on inhibitory cell type and connectivity. (2005) Nature Neuroscience. 8(11):1552-1559. DOI: 10.1038/nn1565

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Genoud, S., Maricic, I., Kumar, V., Gage, F.H. Targeted expression of IGF-1 in the central nervous system fails to protect mice from experimental autoimmune encephalomyelitis. (2005) Journal of Neuroimmunology. 168(1-2):40-5. DOI: 10.1016/j.jneuroim.2005.06.033

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Cheng, L., Samad, O.A., Xu, Y., Mizuguchi, R., Luo, P., Shirasawa, S., Goulding, M., Ma, Q. Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes. (2005) Nature Neuroscience. 8(11):1510-5. DOI: 10.1038/nn1569

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Lie, D.C., Colamarino, S.A., Song, H.J., Désiré, L., Mira, H., Consiglio, A., Lein, E.S., Jessberger, S., Lansford, H., Dearie, A.R., Gage, F.H. Wnt signalling regulates adult hippocampal neurogenesis. (2005) Naturaleza. 437(7063):1370-5. DOI: 10.1038/nature04108

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Singer, O., Marr, R.A., Rockenstein, E., Crews, L., Coufal, N.G., Gage, F.H., Verma, I.M., Masliah, E. Targeting BACE1 with siRNAs ameliorates Alzheimer disease neuropathology in a transgenic model. (2005) Nature Neuroscience. 8(10):1343-9. DOI: 10.1038/nn1531

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Sample, P.A., Boden, C., Zhang, Z., Pascual, J., Lee, T.W., Zangwill, L.M., Weinreb, R.N., Crowston, J.G., Hoffmann, E.M., Medeiros, F.A., Sejnowski, T., Goldbaum, M. Unsupervised machine learning with independent component analysis to identify areas of progression in glaucomatous visual fields. (2005) Investigative Ophthalmology & Visual Science. 46(10):3684-3692. DOI: 10.1167/iovs.04-1168

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Goldbaum, M.H., Sample, P.A., Zhang, Z., Chan, K., Hao, J., Lee, T.W., Boden, C., Bowd, C., Bourne, R., Zangwill, L., Sejnowski, T., Spinak, D., Weinreb, R.N. Using unsupervised learning with independent component analysis to identify patterns of glaucomatous visual field defects. (2005) Investigative Ophthalmology & Visual Science. 46(10):3676-3683. DOI: 10.1167/iovs.04-1167

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Gage, F.H., McAllister, A.K. Neuronal and glial cell biology. (2005) Current Opinion in Neurobiology. 15(5):497-9. DOI: 10.1016/j.conb.2005.08.010

van Praag, H., Shubert, T., Zhao, C., Gage, F.H. Exercise enhances learning and hippocampal neurogenesis in aged mice. (2005) Diario de Neurociencia. 25(38):8680-5. DOI: 10.1523/JNEUROSCI.1731-05.2005

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Cummings, B.J., Uchida, N., Tamaki, S.J., Salazar, D.L., Hooshmand, M., Summers, R., Gage, F.H., Anderson, A.J. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. (2005) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 102(39):14069-74. DOI: 10.1073/pnas.0507063102

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Aigner, S., Gage, F.H. A small gem with great powers: geminin keeps neural progenitors thriving. (2005) Developmental Cell. 9(2):171-2. DOI: 10.1016/j.devcel.2005.07.005

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Sullivan, M.R., Nimmerjahn, A., Sarkisov, D.V., Helmchen, F., Wang, S.S. In vivo calcium imaging of circuit activity in cerebellar cortex. (2005) Journal of Neurophysiology. 94(2):1636-44. DOI: 10.1152/jn.01013.2004

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Gillette, M.U., Sejnowski, T.J. Physiology. Biological clocks coordinately keep life on time. (2005) Ciencia. 309(5738):1196-1198. DOI: 10.1126/science.1111420

Prank, K., Schulze, E., Eckert, O., Nattkemper, T.W., Bettendorf, M., Maser-Gluth, C., Sejnowski, T.J., Grote, A., Penner, E., von Zur Mühlen, A., Brabant, G. Machine learning approaches for phenotype-genotype mapping: predicting heterozygous mutations in the CYP21B gene from steroid profiles. (2005) Eur. J. Endocrinol. 153(2):301-305. DOI: 10.1530/eje.1.01957

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Zupanc, G.K., Hinsch, K., Gage, F.H. Proliferation, migration, neuronal differentiation, and long-term survival of new cells in the adult zebrafish brain. (2005) Journal of Comparative Neurology. 488(3):290-319. DOI: 10.1002/cne.20571

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Coggan, J.S., Bartol, T.M., Esquenazi, E., Stiles, J.R., Lamont, S., Martone, M.E., Berg, D.K., Ellisman, M.H., Sejnowski, T.J. Evidence for ectopic neurotransmission at a neuronal synapse. (2005) Ciencia. 309(5733):446-451. DOI: 10.1126/science.1108239

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Kriks, S., Lanuza, G.M., Mizuguchi, R., Nakafuku, M., Goulding, M. Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord. (2005) Development. 132(13):2991-3002. DOI: 10.1242/dev.01878

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Callaway, E.M. Structure and function of parallel pathways in the primate early visual system. (2005) Journal of Physiology. 566(Pt 1):13-19. DOI: 10.1113/jphysiol.2005.088047

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Muotri, A.R., Chu, V.T., Marchetto, M.C., Deng, W., Moran, J.V., Gage, F.H. Somatic mosaicism in neuronal precursor cells mediated by L1 retrotransposition. (2005) Naturaleza. 435(7044):903-10. DOI: 10.1038/nature03663

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Fröhlich, F., Bazhenov, M., Timofeev, I., Sejnowski, T.J. Maintenance and termination of neocortical oscillations by dynamic modulation of intrinsic and synaptic excitability. (2005) Thalamus Relat Syst. 3(2):147-156. DOI: 10.1017/S147292880700015

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Houweling, A.R., Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Homeostatic synaptic plasticity can explain post-traumatic epileptogenesis in chronically isolated neocortex. (2005) Cerebral Cortex . 15(6):834-845. DOI: 10.1093/cercor/bhh184

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Kuwabara, T., Hsieh, J., Nakashima, K., Warashina, M., Taira, K., Gage, F.H. The NRSE smRNA specifies the fate of adult hippocampal neural stem cells. (2005) Nucleic Acids Symp Ser (Oxf).(49):87-8 DOI: 10.1093/nass/49.1.87

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Lin, W., Dominguez, B., Yang, J., Aryal, P., Brandon, E.P., Gage, F.H., Lee, K.F. Neurotransmitter acetylcholine negatively regulates neuromuscular synapse formation by a Cdk5-dependent mechanism. (2005) Neurona. 46(4):569-79. DOI: 10.1016/j.neuron.2005.04.002

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Bazhenov, M., Stopfer, M., Sejnowski, T.J., Laurent, G. Fast odor learning improves reliability of odor responses in the locust antennal lobe. (2005) Neurona. 46(3):483-492. DOI: 10.1016/j.neuron.2005.03.022

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Kaspar, B.K., Frost, L.M., Christian, L., Umapathi, P., Gage, F.H. Synergy of insulin-like growth factor-1 and exercise in amyotrophic lateral sclerosis. (2005) Ann. Neurol. 57(5):649-55. DOI: 10.1002/ana.20451

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Scheel, J.R., Ray, J., Gage, F.H., Barlow, C. Quantitative analysis of gene expression in living adult neural stem cells by gene trapping. (2005) Nature Methods. 2(5):363-70. DOI: 10.1038/nmeth755

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Briggs, F., Callaway, E.M. Laminar patterns of local excitatory input to layer 5 neurons in macaque primary visual cortex. (2005) Cereb. Cortex. 15(5):479-488. DOI: 10.1093/cercor/bhh154

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Nimmerjahn, A., Kirchhoff, F., Helmchen, F. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. (2005) Ciencia. 308(5726):1314-8. DOI: 10.1126/science.1110647

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Duann, J.R., Jung, T.P., Makeig, S., Sejnowski, T. Repeated decompositions reveal the stability of infomax decomposition of fMRI data. (2005) Conf Proc IEEE Eng Med Biol Soc. 5:5324-5327. DOI: 10.1109/IEMBS.2005.1615683

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Miller, T.M., Kaspar, B.K., Kops, G.J., Yamanaka, K., Christian, L.J., Gage, F.H., Cleveland, D.W. Virus-delivered small RNA silencing sustains strength in amyotrophic lateral sclerosis. (2005) Ann. Neurol. 57(5):773-6. DOI: 10.1002/ana.20453

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Lilley, C.E., Carson, C.T., Muotri, A.R., Gage, F.H., Weitzman, M.D. DNA repair proteins affect the lifecycle of herpes simplex virus 1. (2005) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 102(16):5844-9. DOI: 10.1073/pnas.0501916102

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Myers, C.P., Lewcock, J.W., Hanson, M.G., Gosgnach, S., Aimone, J.B., Gage, F.H., Lee, K.F., Landmesser, L.T., Pfaff, S.L. Cholinergic input is required during embryonic development to mediate proper assembly of spinal locomotor circuits. (2005) Neurona. 46(1):37-49. DOI: 10.1016/j.neuron.2005.02.022

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Marquardt, T., Shirasaki, R., Ghosh, S., Andrews, S.E., Carter, N., Hunter, T., Pfaff, S.L. Coexpressed EphA receptors and ephrin-A ligands mediate opposing actions on growth cone navigation from distinct membrane domains. (2005) Móvil. 121(1):127-39. DOI: 10.1016/j.cell.2005.01.020

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Borrell, V., Yoshimura, Y., Callaway, E.M. Targeted gene delivery to telencephalic inhibitory neurons by directional in utero electroporation. (2005) Journal of Neuroscience Methods. 143(2):151-158. DOI: 10.1016/j.jneumeth.2004.09.027

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Callaway, E.M. A molecular and genetic arsenal for systems neuroscience. (2005) Trends in Neurosciences. 28(4):196-201. DOI: 10.1016/j.tins.2005.01.007

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Lein, E.S., Callaway, E.M., Albright, T.D., Gage, F.H. Redefining the boundaries of the hippocampal CA2 subfield in the mouse using gene expression and 3-dimensional reconstruction. (2005) Journal of Comparative Neurology. 485(1):1-10. DOI: 10.1002/cne.20426

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Bowd, C., Medeiros, F.A., Zhang, Z., Zangwill, L.M., Hao, J., Lee, T.W., Sejnowski, T.J., Weinreb, R.N., Goldbaum, M.H. Relevance vector machine and support vector machine classifier analysis of scanning laser polarimetry retinal nerve fiber layer measurements. (2005) Investigative Ophthalmology & Visual Science. 46(4):1322-1329. DOI: 10.1167/iovs.04-1122

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Galimi, F., Summers, R.G., van Praag, H., Verma, I.M., Gage, F.H. A role for bone marrow-derived cells in the vasculature of noninjured CNS. (2005) Blood. 105(6):2400-2. DOI: 10.1182/blood-2004-02-0612

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Yoshimura, Y., Dantzker, J.L., Callaway, E.M. Excitatory cortical neurons form fine-scale functional networks. (2005) Naturaleza. 433(7028):868-873. DOI: 10.1038/nature03252

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Goulding, M., Pfaff, S.L. Development of circuits that generate simple rhythmic behaviors in vertebrates. (2005) Current Opinion in Neurobiology. 15(1):14-20. DOI: 10.1016/j.conb.2005.01.017

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Lee, S.K., Jurata, L.W., Nowak, R., Lettieri, K., Kenny, D.A., Pfaff, S.L., Gill, G.N. The LIM domain-only protein LMO4 is required for neural tube closure. (2005) Molecular and Cellular Neurosciences. 28(2):205-14. DOI: 10.1016/j.mcn.2004.04.010

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Martin, M.J., Muotri, A., Gage, F., Varki, A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. (2005) Medicina Natural. 11(2):228-32. DOI: 10.1038/nm1181

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Callaway, E.M. Neural substrates within primary visual cortex for interactions between parallel visual pathways. (2005) Progress in Brain Research. 149:59-64. DOI: 10.1016/S0079-6123(05)49005-6

Lee, S.K., Lee, B., Ruiz, E.C., Pfaff, S.L. Olig2 and Ngn2 function in opposition to modulate gene expression in motor neuron progenitor cells. (2005) Genes y Desarrollo. 19(2):282-94. DOI: 10.1101/gad.1257105

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Yeo, M., Lee, S.K., Lee, B., Ruiz, E.C., Pfaff, S.L., Gill, G.N. Small CTD phosphatases function in silencing neuronal gene expression. (2005) Ciencia. 307(5709):596-600. DOI: 10.1126/science.1100801

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Cepeda-Nieto, A.C., Pfaff, S.L., Varela-Echavarría, A. Homeodomain transcription factors in the development of subsets of hindbrain reticulospinal neurons. (2005) Molecular and Cellular Neurosciences. 28(1):30-41. DOI: 10.1016/j.mcn.2004.06.016

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Lehky, S.R., Sejnowski, T.J., Desimone, R. Selectivity and sparseness in the responses of striate complex cells. (2005) Vision Research. 45(1):57-73. DOI: 10.1016/j.visres.2004.07.021

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Fuentealba, P., Timofeev, I., Bazhenov, M., Sejnowski, T.J., Steriade, M. Membrane bistability in thalamic reticular neurons during spindle oscillations. (2005) Journal of Neurophysiology. 93(1):294-304. DOI: 10.1152/jn.00552.2004

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Siegel, R.M., Callaway, E.M. Francis Crick's legacy for neuroscience: between the alpha and the Omega. (2004) PLOS Biología. 2(12). DOI: 10.1371/journal.pbio.0020419

Dittgen, T., Nimmerjahn, A., Komai, S., Licznerski, P., Waters, J., Margrie, T.W., Helmchen, F., Denk, W., Brecht, M., Osten, P. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. (2004) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 101(52):18206-11. DOI: 10.1073/pnas.0407976101

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Hashimoto, M., Rockenstein, E., Mante, M., Crews, L., Bar-On, P., Gage, F.H., Marr, R., Masliah, E. An antiaggregation gene therapy strategy for Lewy body disease utilizing beta-synuclein lentivirus in a transgenic model. (2004) Gene Therapy. 11(23):1713-23. DOI: 10.1038/sj.gt.3302349

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Hsieh, J., Nakashima, K., Kuwabara, T., Mejia, E., Gage, F.H. Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells. (2004) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 101(47):16659-64. DOI: 10.1073/pnas.0407643101

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Pak, W., Hindges, R., Lim, Y.S., Pfaff, S.L., O'Leary, D.D. Magnitude of binocular vision controlled by islet-2 repression of a genetic program that specifies laterality of retinal axon pathfinding. (2004) Móvil. 119(4):567-78. DOI: 10.1016/j.cell.2004.10.026

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Göbel, W., Kerr, J.N., Nimmerjahn, A., Helmchen, F. Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective. (2004) Opt Lett. 29(21):2521-3.

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Chin, V.I., Taupin, P., Sanga, S., Scheel, J., Gage, F.H., Bhatia, S.N. Microfabricated platform for studying stem cell fates. (2004) Biotechnol. Bioeng. 88(3):399-415. DOI: 10.1002/bit.20254

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Platforms that allow parallel, quantitative analysis of single cells will be integral to realizing the potential of postgenomic biology. In stem cell biology, the study of clonal stem cells in multiwell formats is currently both inefficient and time-consuming. Thus, to investigate low-frequency events of interest, large sample sizes must be interrogated. We report a simple, versatile, and efficient micropatterned arraying system conducive to the culture and dynamic monitoring of stem cell proliferation. This platform enables: 1) parallel, automated, long-term ( approximately days to weeks), live-cell microscopy of single cells in culture; 2) tracking of individual cell fates over time (proliferation, apoptosis); and 3) correlation of differentiated progeny with founder clones. To achieve these goals, we used microfabrication techniques to create an array of approximately 10,000 microwells on a glass coverslip. The dimensions of the wells are tunable, ranging from 20 to >500 microm in diameter and 10-500 microm in height. The microarray can be coated with adhesive proteins and is integrated into a culture chamber that permits rapid (approximately min), addressable monitoring of each well using a standard programmable microscope stage. All cells share the same media (including paracrine survival signals), as opposed to cells in multiwell formats. The incorporation of a coverslip as a substrate also renders the platform compatible with conventional, high-magnification light and fluorescent microscopy. We validated this approach by analyzing the proliferation dynamics of a heterogeneous adult rat neural stem cell population. Using this platform, one can further interrogate the response of distinct stem cell subpopulations to microenvironmental cues (mitogens, cell-cell interactions, and cell-extracellular matrix interactions) that govern their behavior. In the future, the platform may also be adapted for the study of other cell types by tailoring the surface coatings, microwell dimensions, and culture environment, thereby enabling parallel investigation of many distinct cellular responses.

Shihabuddin, L.S., Numan, S., Huff, M.R., Dodge, J.C., Clarke, J., Macauley, S.L., Yang, W., Taksir, T.V., Parsons, G., Passini, M.A., Gage, F.H., Stewart, G.R. Intracerebral transplantation of adult mouse neural progenitor cells into the Niemann-Pick-A mouse leads to a marked decrease in lysosomal storage pathology. (2004) Diario de Neurociencia. 24(47):10642-51. DOI: 10.1523/JNEUROSCI.3584-04.2004

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Hsieh, J., Gage, F.H. Epigenetic control of neural stem cell fate. (2004) Current Opinion in Genetics & Development. 14(5):461-9. DOI: 10.1016/j.gde.2004.07.006

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Horwitz, G.D., Callaway, E.M. Antisense inhibition of reward learning. (2004) Nature Neuroscience. 7(10):1023-1024. DOI: 10.1038/nn1004-1023

Nimmerjahn, A., Kirchhoff, F., Kerr, J.N., Helmchen, F. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. (2004) Nature Methods. 1(1):31-7. DOI: 10.1038/nmeth706

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Consiglio, A., Gritti, A., Dolcetta, D., Follenzi, A., Bordignon, C., Gage, F.H., Vescovi, A.L., Naldini, L. Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors. (2004) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 101(41):14835-40. DOI: 10.1073/pnas.0404180101

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Schinder, A.F., Gage, F.H. A hypothesis about the role of adult neurogenesis in hippocampal function. (2004) Physiology (Bethesda). 19:253-61. DOI: 10.1152/physiol.00012.2004

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Aimone, J.B., Leasure, J.L., Perreau, V.M., Thallmair, M., Gage Spatial and temporal gene expression profiling of the contused rat spinal cord. (2004) Exp. Neurol. 189(2):204-21. DOI: 10.1016/j.expneurol.2004.05.042

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Sejnowski, T.J. In memoriam: Francis H.C. Crick. (2004) Neurona. 43(5):619-621. DOI: 10.1016/j.neuron.2004.08.022

Goulding, M. How early is firing required for wiring? (2004) Neurona. 43(5):601-3. DOI: 10.1016/j.neuron.2004.08.027

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Schwander, M., Shirasaki, R., Pfaff, S.L., Müller, U. Beta1 integrins in muscle, but not in motor neurons, are required for skeletal muscle innervation. (2004) Diario de Neurociencia. 24(37):8181-91. DOI: 10.1523/JNEUROSCI.1345-04.2004

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Weiner, J.A., Koo, S.J., Nicolas, S., Fraboulet, S., Pfaff, S.L., Pourquié, O., Sanes, J.R. Axon fasciculation defects and retinal dysplasias in mice lacking the immunoglobulin superfamily adhesion molecule BEN/ALCAM/SC1. (2004) Molecular and Cellular Neurosciences. 27(1):59-69. DOI: 10.1016/j.mcn.2004.06.005

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Zangwill, L.M., Chan, K., Bowd, C., Hao, J., Lee, T.W., Weinreb, R.N., Sejnowski, T.J., Goldbaum, M.H. Heidelberg retina tomograph measurements of the optic disc and parapapillary retina for detecting glaucoma analyzed by machine learning classifiers. (2004) Investigative Ophthalmology & Visual Science. 45(9):3144-3151. DOI: 10.1167/iovs.04-0202

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Potassium model for slow (2-3 Hz) in vivo neocortical paroxysmal oscillations. (2004) Journal of Neurophysiology. 92(2):1116-1132. DOI: 10.1152/jn.00529.2003

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In slow neocortical paroxysmal oscillations, the de- and hyperpolarizing envelopes in neocortical neurons are large compared with slow sleep oscillations. Increased local synchrony of membrane potential oscillations during seizure is reflected in larger electroencephalographic oscillations and the appearance of spike- or polyspike-wave complex recruitment at 2- to 3-Hz frequencies. The oscillatory mechanisms underlying this paroxysmal activity were investigated in computational models of cortical networks. The extracellular K(+) concentration ([K(+)](o)) was continuously computed based on neuronal K(+) currents and K(+) pumps as well as glial buffering. An increase of [K(+)](o) triggered a transition from normal awake-like oscillations to 2- to 3-Hz seizure-like activity. In this mode, the cells fired periodic bursts and nearby neurons oscillated highly synchronously; in some cells depolarization led to spike inactivation lasting 50-100 ms. A [K(+)](o) increase, sufficient to produce oscillations could result from excessive firing (e.g., induced by external stimulation) or inability of K(+) regulatory system (e.g., when glial buffering was blocked). A combination of currents including high-threshold Ca(2+), persistent Na(+) and hyperpolarization-activated depolarizing (I(h)) currents was sufficient to maintain 2- to 3-Hz activity. In a network model that included lateral K(+) diffusion between cells, increase of [K(+)](o) in a small region was generally sufficient to maintain paroxysmal oscillations in the whole network. Slow changes of [K(+)](o) modulated the frequency of bursting and, in some case, led to fast oscillations in the 10- to 15-Hz frequency range, similar to the fast runs observed during seizures in vivo. These results suggest that modifications of the intrinsic currents mediated by increase of [K(+)](o) can explain the range of neocortical paroxysmal oscillations in vivo.

Sample, P.A., Chan, K., Boden, C., Lee, T.W., Blumenthal, E.Z., Weinreb, R.N., Bernd, A., Pascual, J., Hao, J., Sejnowski, T., Goldbaum, M.H. Using unsupervised learning with variational bayesian mixture of factor analysis to identify patterns of glaucomatous visual field defects. (2004) Investigative Ophthalmology & Visual Science. 45(8):2596-2605. DOI: 10.1167/iovs.03-0343

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Shon, A.P., Rao, R.P., Sejnowski, T.J. Motion detection and prediction through spike-timing dependent plasticity. (2004) Network. 15(3):179-198.

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Callaway, E.M. Close encounters: how cortical neurons find and connect to their correct synaptic partners depends on the cell type. (2004) Neurona. 43(2):156-158. DOI: 10.1016/j.neuron.2004.07.006

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Wurmser, A.E., Nakashima, K., Summers, R.G., Toni, N., D'Amour, K.A., Lie, D.C., Gage, F.H. Cell fusion-independent differentiation of neural stem cells to the endothelial lineage. (2004) Naturaleza. 430(6997):350-6. DOI: 10.1038/nature02604

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Lee, S.K., Jurata, L.W., Funahashi, J., Ruiz, E.C., Pfaff, S.L. Analysis of embryonic motoneuron gene regulation: derepression of general activators function in concert with enhancer factors. (2004) Development. 131(14):3295-306. DOI: 10.1242/dev.01179

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Tiesinga, P.H., Fellous, J.M., Salinas, E., José, J.V., Sejnowski, T.J. Inhibitory synchrony as a mechanism for attentional gain modulation. (2004) Journal of Physiology, Paris. 98(4-6):296-314. DOI: 10.1016/j.jphysparis.2005.09.002

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Recordings from area V4 of monkeys have revealed that when the focus of attention is on a visual stimulus within the receptive field of a cortical neuron, two distinct changes can occur: The firing rate of the neuron can change and there can be an increase in the coherence between spikes and the local field potential (LFP) in the gamma-frequency range (30-50 Hz). The hypothesis explored here is that these observed effects of attention could be a consequence of changes in the synchrony of local interneuron networks. We performed computer simulations of a Hodgkin-Huxley type neuron driven by a constant depolarizing current, I, representing visual stimulation and a modulatory inhibitory input representing the effects of attention via local interneuron networks. We observed that the neuron's firing rate and the coherence of its output spike train with the synaptic inputs was modulated by the degree of synchrony of the inhibitory inputs. When inhibitory synchrony increased, the coherence of spiking model neurons with the synaptic input increased, but the firing rate either increased or remained the same. The mean number of synchronous inhibitory inputs was a key determinant of the shape of the firing rate versus current (f-I) curves. For a large number of inhibitory inputs (approximately 50), the f-I curve saturated for large I and an increase in input synchrony resulted in a shift of sensitivity-the model neuron responded to weaker inputs I. For a small number (approximately 10), the f-I curves were non-saturating and an increase in input synchrony led to an increase in the gain of the response-the firing rate in response to the same input was multiplied by an approximately constant factor. The firing rate modulation with inhibitory synchrony was highest when the input network oscillated in the gamma frequency range. Thus, the observed changes in firing rate and coherence of neurons in the visual cortex could be controlled by top-down inputs that regulated the coherence in the activity of a local inhibitory network discharging at gamma frequencies.

Fuentealba, P., Crochet, S., Timofeev, I., Bazhenov, M., Sejnowski, T.J., Steriade, M. Experimental evidence and modeling studies support a synchronizing role for electrical coupling in the cat thalamic reticular neurons in vivo. (2004) European Journal of Neuroscience. 20(1):111-9. DOI: 10.1111/j.1460-9568.2004.03462.x

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Bowd, C., Zangwill, L.M., Medeiros, F.A., Hao, J., Chan, K., Lee, T.W., Sejnowski, T.J., Goldbaum, M.H., Sample, P.A., Crowston, J.G., Weinreb, R.N. Confocal scanning laser ophthalmoscopy classifiers and stereophotograph evaluation for prediction of visual field abnormalities in glaucoma-suspect eyes. (2004) Investigative Ophthalmology & Visual Science. 45(7):2255-2262.

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Pizzo, D.P., Paban, V., Coufal, N.G., Gage, F.H., Thal, L.J. Long-term production of choline acetyltransferase in the CNS after transplantation of fibroblasts modified with a regulatable vector. (2004) Brain Research. Molecular Brain Research. 126(1):1-13. DOI: 10.1016/j.molbrainres.2004.03.006

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Farmer, J., Zhao, X., van Praag, H., Wodtke, K., Gage, F.H., Christie, B.R. Effects of voluntary exercise on synaptic plasticity and gene expression in the dentate gyrus of adult male Sprague-Dawley rats in vivo. (2004) Neuroscience. 124(1):71-9. DOI: 10.1016/j.neuroscience.2003.09.029

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Callaway, E.M. Feedforward, feedback and inhibitory connections in primate visual cortex. (2004) Neural Networks. 17(5-6):625-632. DOI: 10.1016/j.neunet.2004.04.004

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Brandon, E.P., Mellott, T., Pizzo, D.P., Coufal, N., D'Amour, K.A., Gobeske, K., Lortie, M., López-Coviella, I., Berse, B., Thal, L.J., Gage, F.H., Blusztajn, J.K. Choline transporter 1 maintains cholinergic function in choline acetyltransferase haploinsufficiency. (2004) Diario de Neurociencia. 24(24):5459-66. DOI: 10.1523/JNEUROSCI.1106-04.2004

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Gage, F.H. Structural plasticity of the adult brain. (2004) Dialogues Clin Neurosci. 6(2):135-41.

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Schwartz, O., Movellan, J.R., Wachtler, T., Albright, T.D., Sejnowski, T.J. Spike count distributions, factorizability, and contextual effects in area V1. (2004) Neurocomputing. 58-60:893-900. DOI: 10.1016/j.neucom.2004.01.143

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Arnold, M., Sejnowski, T., Hammerstrom, D., Jabri, M. Neural systems integration. (2004) Neurocomputing. 58-60:1123-1128. DOI: 10.1016/j.neucom.2004.01.176

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Lie, D.C., Song, H., Colamarino, S.A., Ming, G.L., Gage, F.H. Neurogenesis in the adult brain: new strategies for central nervous system diseases. (2004) Annual Review of Pharmacology and Toxicology. 44:399-421. DOI: 10.1146/annurev.pharmtox.44.101802.121631

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Marr, R.A., Guan, H., Rockenstein, E., Kindy, M., Gage, F.H., Verma, I., Masliah, E., Hersh, L.B. Neprilysin regulates amyloid Beta peptide levels. (2004) J. Mol. Neurosci. 22(1-2):5-11. DOI: 10.1385/JMN:22:1-2:5

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Goulding, M. Neurobiology: a matter of balance. (2004) Naturaleza. 429(6991):515-7. DOI: 10.1038/429515a

Göbel, W., Nimmerjahn, A., Helmchen, F. Distortion-free delivery of nanojoule femtosecond pulses from a Ti:sapphire laser through a hollow-core photonic crystal fiber. (2004) Optics Letters. 29(11):1285-7.

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Makeig, S., Delorme, A., Westerfield, M., Jung, T.P., Townsend, J., Courchesne, E., Sejnowski, T.J. Electroencephalographic brain dynamics following manually responded visual targets. (2004) PLOS Biología. 2(6):e176. DOI: 10.1371/journal.pbio.0020176

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Tiesinga, P.H., Fellous, J.M., Salinas, E., José, J.V., Sejnowski, T.J. Synchronization as a mechanism for attentional gain modulation. (2004) Neurocomputing. 58-60:641-646. DOI: 10.1016/j.neucom.2004.01.108

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Schaffer, D.V., Gage, F.H. Neurogenesis and neuroadaptation. (2004) Neuromolecular Med. 5(1):1-9. DOI: 10.1385/NMM:5:1:001

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Sharpee, T., Sugihara, H., Kurgansky, A.V., Rebrik, S., Stryker, M.P., Miller, K.D. Probing feature selectivity of neurons in primary visual cortex with natural stimuli. (2004) Proc SPIE Int Soc Opt Eng.(5467):212-222

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Wurmser, A.E., Palmer, T.D., Gage, F.H. Neuroscience. Cellular interactions in the stem cell niche. (2004) Ciencia. 304(5675):1253-5. DOI: 10.1126/science.1099344

Lanuza, G.M., Gosgnach, S., Pierani, A., Jessell, T.M., Goulding, M. Genetic identification of spinal interneurons that coordinate left-right locomotor activity necessary for walking movements. (2004) Neurona. 42(3):375-86.

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Aimone, J.B., Gage, F.H. Unbiased characterization of high-density oligonucleotide microarrays using probe-level statistics. (2004) Journal of Neuroscience Methods. 135(1-2):27-33. DOI: 10.1016/j.jneumeth.2003.11.016

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Cheng, L., Arata, A., Mizuguchi, R., Qian, Y., Karunaratne, A., Gray, P.A., Arata, S., Shirasawa, S., Bouchard, M., Luo, P., Chen, C.L., Busslinger, M., Goulding, M., Onimaru, H., Ma, Q. Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates. (2004) Nature Neuroscience. 7(5):510-7. DOI: 10.1038/nn1221

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Lein, E.S., Zhao, X., Gage, F.H. Defining a molecular atlas of the hippocampus using DNA microarrays and high-throughput in situ hybridization. (2004) Diario de Neurociencia. 24(15):3879-89. DOI: 10.1523/JNEUROSCI.4710-03.2004

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Yates, P.A., Holub, A.D., McLaughlin, T., Sejnowski, T.J., O'Leary, D.D. Computational modeling of retinotopic map development to define contributions of EphA-ephrinA gradients, axon-axon interactions, and patterned activity. (2004) Journal of Neurobiology. 59(1):95-113. DOI: 10.1002/neu.10341

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The topographic projection of retinal ganglion cell (RGC) axons to mouse superior colliculus (SC) or chick optic tectum (OT) is formed in three phases: RGC axons overshoot their termination zone (TZ); they exhibit interstitial branching along the axon that is topographically biased for the correct location of their future TZ; and branches arborize preferentially at the TZ and the initial exuberant projection refines through axon and branch elimination to generate a precise retinotopic map. We present a computational model of map development that demonstrates that the countergradients of EphAs and ephrinAs in retina and the OT/SC and bidirectional repellent signaling between RGC axons and OT/SC cells are sufficient to direct an initial topographic bias in RGC axon branching. Our model also suggests that a proposed repellent action of EphAs/ephrinAs present on RGC branches and arbors added to that of EphAs/ephrinAs expressed by OT/SC cells is required to progressively restrict branching and arborization to topographically correct locations and eliminate axon overshoot. Simulations show that this molecular framework alone can develop considerable topographic order and refinement, including axon elimination, a feature not programmed into the model. Generating a refined map with a condensed TZ as in vivo requires an additional parameter that enhances branch formation along an RGC axon near sites that it has a higher branch density, and resembles an assumed role for patterned neural activity. The same computational model generates the phenotypes reported in ephrinA deficient mice and Isl2-EphA3 knockin mice. This modeling suggests that gradients of counter-repellents can establish a substantial degree of topographic order in the OT/SC, and that repellents present on RGC axon branches and arbors make a substantial contribution to map refinement. However, competitive interactions between RGC axons that enhance the probability of continued local branching are required to generate precise retinotopy.

Eagleman, D.M., Jacobson, J.E., Sejnowski, T.J. Perceived luminance depends on temporal context. (2004) Naturaleza. 428(6985):854-856. DOI: 10.1038/nature02467

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Kempermann, G., Wiskott, L., Gage, F.H. Functional significance of adult neurogenesis. (2004) Current Opinion in Neurobiology. 14(2):186-91. DOI: 10.1016/j.conb.2004.03.001

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Fellous, J.M., Tiesinga, P.H., Thomas, P.J., Sejnowski, T.J. Discovering spike patterns in neuronal responses. (2004) Diario de Neurociencia. 24(12):2989-3001. DOI: 10.1523/JNEUROSCI.4649-03.2004

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Kuwabara, T., Hsieh, J., Nakashima, K., Taira, K., Gage, F.H. A small modulatory dsRNA specifies the fate of adult neural stem cells. (2004) Móvil. 116(6):779-93.

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Markakis, E.A., Palmer, T.D., Randolph-Moore, L., Rakic, P., Gage, F.H. Novel neuronal phenotypes from neural progenitor cells. (2004) Diario de Neurociencia. 24(12):2886-97. DOI: 10.1523/JNEUROSCI.4161-03.2004

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Thaler, J.P., Koo, S.J., Kania, A., Lettieri, K., Andrews, S., Cox, C., Jessell, T.M., Pfaff, S.L. A postmitotic role for Isl-class LIM homeodomain proteins in the assignment of visceral spinal motor neuron identity. (2004) Neurona. 41(3):337-50.

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Sapir, T., Geiman, E.J., Wang, Z., Velasquez, T., Mitsui, S., Yoshihara, Y., Frank, E., Alvarez, F.J., Goulding, M. Pax6 and engrailed 1 regulate two distinct aspects of renshaw cell development. (2004) Diario de Neurociencia. 24(5):1255-64. DOI: 10.1523/JNEUROSCI.3187-03.2004

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Sharpee, T., Rust, N.C., Bialek, W. Analyzing neural responses to natural signals: maximally informative dimensions. (2004) Computación neuronal. 16(2):223-50. DOI: 10.1162/089976604322742010

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Brivanlou, I.H., Dantzker, J.L., Stevens, C.F., Callaway, E.M. Topographic specificity of functional connections from hippocampal CA3 to CA1. (2004) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 101(8):2560-2565.

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Kim, J., Dittgen, T., Nimmerjahn, A., Waters, J., Pawlak, V., Helmchen, F., Schlesinger, S., Seeburg, P.H., Osten, P. Sindbis vector SINrep(nsP2S726): a tool for rapid heterologous expression with attenuated cytotoxicity in neurons. (2004) Journal of Neuroscience Methods. 133(1-2):81-90.

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Tiesinga, P.H., Sejnowski, T.J. Rapid temporal modulation of synchrony by competition in cortical interneuron networks. (2004) Computación neuronal. 16(2):251-75. DOI: 10.1162/089976604322742029

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Hsieh, J., Aimone, J.B., Kaspar, B.K., Kuwabara, T., Nakashima, K., Gage, F.H. IGF-I instructs multipotent adult neural progenitor cells to become oligodendrocytes. (2004) Revista de Biología Celular. 164(1):111-22. DOI: 10.1083/jcb.200308101

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Shi, Y., Chichung Lie, D., Taupin, P., Nakashima, K., Ray, J., Yu, R.T., Gage, F.H., Evans, R.M. Expression and function of orphan nuclear receptor TLX in adult neural stem cells. (2004) Naturaleza. 427(6969):78-83. DOI: 10.1038/nature02211

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Schreiber, S., Fellous, J.M., Tiesinga, P., Sejnowski, T.J. Influence of ionic conductances on spike timing reliability of cortical neurons for suprathreshold rhythmic inputs. (2004) Journal of Neurophysiology. 91(1):194-205. DOI: 10.1152/jn.00556.2003

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Nakashima, K., Colamarino, S., Gage, F.H. Embryonic stem cells: staying plastic on plastic. (2004) Medicina Natural. 10(1):23-4. DOI: 10.1038/nm0104-23

Chatterjee, S., Callaway, E.M. Parallel colour-opponent pathways to primary visual cortex. (2003) Naturaleza. 426(6967):668-671. DOI: 10.1038/nature02167

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Cai, C.L., Liang, X., Shi, Y., Chu, P.H., Pfaff, S.L., Chen, J., Evans, S. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart. (2003) Developmental Cell. 5(6):877-89.

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Fellous, J.M., Sejnowski, T.J. Regulation of persistent activity by background inhibition in an in vitro model of a cortical microcircuit. (2003) Cerebral Cortex . 13(11):1232-1241.

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Anemüller, J., Sejnowski, T.J., Makeig, S. Complex independent component analysis of frequency-domain electroencephalographic data. (2003) Neural Networks. 16(9):1311-1323. DOI: 10.1016/j.neunet.2003.08.003

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Muotri, A.R., Gage, F.H. Vibrating in the background. (2003) Nature Genetics. 35(3):205-7. DOI: 10.1038/ng1103-205

Sejnowski, T. The computational self. (2003) Ann. N. Y. Acad. Sci. 1001:262-271.

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Rhodes, J.S., van Praag, H., Jeffrey, S., Girard, I., Mitchell, G.S., Garland, T., Gage, F.H. Exercise increases hippocampal neurogenesis to high levels but does not improve spatial learning in mice bred for increased voluntary wheel running. (2003) Behavioral Neuroscience. 117(5):1006-16. DOI: 10.1037/0735-7044.117.5.1006

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Dziewczapolski, G., Lie, D.C., Ray, J., Gage, F.H., Shults, C.W. Survival and differentiation of adult rat-derived neural progenitor cells transplanted to the striatum of hemiparkinsonian rats. (2003) Exp. Neurol. 183(2):653-64.

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Destexhe, A., Sejnowski, T.J. Interactions between membrane conductances underlying thalamocortical slow-wave oscillations. (2003) Physiological Reviews. 83(4):1401-1453. DOI: 10.1152/physrev.00012.2003

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Gage, F.H. Brain, repair yourself. (2003) Sci. Am. 289(3):46-53.

D'Amour, K.A., Gage, F.H. Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells. (2003) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 100 Suppl :11866-72. DOI: 10.1073/pnas.1834200100

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Gage, F.H., Verma, I.M. Stem cells at the dawn of the 21st century. (2003) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 100 Suppl :11817-8. DOI: 10.1073/pnas.1834433100

Laughlin, S.B., Sejnowski, T.J. Communication in neuronal networks. (2003) Ciencia. 301(5641):1870-1874. DOI: 10.1126/science.1089662

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Aberg, M.A., Aberg, N.D., Palmer, T.D., Alborn, A.M., Carlsson-Skwirut, C., Bang, P., Rosengren, L.E., Olsson, T., Gage, F.H., Eriksson, P.S. IGF-I has a direct proliferative effect in adult hippocampal progenitor cells. (2003) Molecular and Cellular Neurosciences. 24(1):23-40.

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Arvanian, V.L., Horner, P.J., Gage, F.H., Mendell, L.M. Chronic neurotrophin-3 strengthens synaptic connections to motoneurons in the neonatal rat. (2003) Diario de Neurociencia. 23(25):8706-12.

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Eagleman, D.M., Sejnowski, T.J. The line-motion illusion can be reversed by motion signals after the line disappears. (2003) Perception. 32(8):963-968. DOI: 10.1068/p3314a

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Kaspar, B.K., Lladó, J., Sherkat, N., Rothstein, J.D., Gage, F.H. Retrograde viral delivery of IGF-1 prolongs survival in a mouse ALS model. (2003) Ciencia. 301(5634):839-42. DOI: 10.1126/science.1086137

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Sejnowski, T.J. Perceptions of science. Tap into science 24-7. (2003) Ciencia. 301(5633):601. DOI: 10.1126/science.1084463

Dickinson-Anson, H., Winkler, J., Fisher, L.J., Song, H.J., Poo, M.m., Gage, F.H. Acetylcholine-secreting cells improve age-induced memory deficits. (2003) Molecular Therapy. 8(1):51-61.

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Lee, S.K., Pfaff, S.L. Synchronization of neurogenesis and motor neuron specification by direct coupling of bHLH and homeodomain transcription factors. (2003) Neurona. 38(5):731-45.

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Kretzberg, J., Sejnowski, T., Warzecha, A.K., Egelhaaf, M. Variability of postsynaptic responses depends non-linearly on the number of synaptic inputs. (2003) Neurocomputing. 52-54:313-320. DOI: 10.1016/S0925-2312(02)00797-X

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Schreiber, S., Fellous, J.M., Whitmer, D., Tiesinga, P., Sejnowski, T.J. A new correlation-based measure of spike timing reliability. (2003) Neurocomputing. 52-54:925-931. DOI: 10.1016/S0925-2312(02)00838-X

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Rao, R.P., Sejnowski, T.J. Self-organizing neural systems based on predictive learning. (2003) Philos Trans A Math Phys Eng Sci. 361(1807):1149-1175. DOI: 10.1098/rsta.2003.1190

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Thomas, P.J., Tiesinga, P.H., Fellous, J.M., Sejnowski, T.J. Reliability and bifurcation in neurons driven by multiple sinusoids. (2003) Neurocomputing. 52-54:955-961. DOI: 10.1016/S0925-2312(02)00856-1

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South, S.M., Kohno, T., Kaspar, B.K., Hegarty, D., Vissel, B., Drake, C.T., Ohata, M., Jenab, S., Sailer, A.W., Malkmus, S., Masuyama, T., Horner, P., Bogulavsky, J., Gage, F.H., Yaksh, T.L., Woolf, C.J., Heinemann, S.F., Inturrisi, C.E. A conditional deletion of the NR1 subunit of the NMDA receptor in adult spinal cord dorsal horn reduces NMDA currents and injury-induced pain. (2003) Diario de Neurociencia. 23(12):5031-40.

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Zhao, X., Ueba, T., Christie, B.R., Barkho, B., McConnell, M.J., Nakashima, K., Lein, E.S., Eadie, B.D., Willhoite, A.R., Muotri, A.R., Summers, R.G., Chun, J., Lee, K.F., Gage, F.H. Mice lacking methyl-CpG binding protein 1 have deficits in adult neurogenesis and hippocampal function. (2003) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 100(11):6777-82. DOI: 10.1073/pnas.1131928100

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Scheel, J.R., Garrett, L.J., Allen, D.M., Carter, T.A., Randolph-Moore, L., Gambello, M.J., Gage, F.H., Wynshaw-Boris, A., Barlow, C. An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues. (2003) Investigación de Ácidos Nucleicos. 31(10):e57.

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Klassen, H.J., Imfeld, K.L., Kirov, I.I., Tai, L., Gage, F.H., Young, M.J., Berman, M.A. Expression of cytokines by multipotent neural progenitor cells. (2003) Cytokine. 22(3-4):101-6.

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Brown, J., Cooper-Kuhn, C.M., Kempermann, G., Van Praag, H., Winkler, J., Gage, F.H., Kuhn, H.G. Enriched environment and physical activity stimulate hippocampal but not olfactory bulb neurogenesis. (2003) European Journal of Neuroscience. 17(10):2042-6.

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Brivanlou, A.H., Gage, F.H., Jaenisch, R., Jessell, T., Melton, D., Rossant, J. Stem cells. Setting standards for human embryonic stem cells. (2003) Ciencia. 300(5621):913-6. DOI: 10.1126/science.1082940

Neubig, M., Destexhe, A., Sejnowski, T.J. Variability of quantal synaptic currents in thalamocortical neurons. (2003) Thalamus Relat Syst. 2(2):153-168. DOI: 10.1016/S1472-9288(03)00008-6

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Franks, K.M., Stevens, C.F., Sejnowski, T.J. Independent sources of quantal variability at single glutamatergic synapses. (2003) Diario de Neurociencia. 23(8):3186-3195.

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Klassen, H., Imfeld, K.L., Ray, J., Young, M.J., Gage, F.H., Berman, M.A. The immunological properties of adult hippocampal progenitor cells. (2003) Vision Research. 43(8):947-56.

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Fellous, J.M., Rudolph, M., Destexhe, A., Sejnowski, T.J. Synaptic background noise controls the input/output characteristics of single cells in an in vitro model of in vivo activity. (2003) Neuroscience. 122(3):811-829.

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Marr, R.A., Rockenstein, E., Mukherjee, A., Kindy, M.S., Hersh, L.B., Gage, F.H., Verma, I.M., Masliah, E. Neprilysin gene transfer reduces human amyloid pathology in transgenic mice. (2003) Diario de Neurociencia. 23(6):1992-6.

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Wachtler, T., Sejnowski, T.J., Albright, T.D. Representation of color stimuli in awake macaque primary visual cortex. (2003) Neurona. 37(4):681-691.

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Doi, E., Inui, T., Lee, T.W., Wachtler, T., Sejnowski, T.J. Spatiochromatic receptive field properties derived from information-theoretic analyses of cone mosaic responses to natural scenes. (2003) Computación neuronal. 15(2):397-417. DOI: 10.1162/089976603762552960

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Kreutz-Delgado, K., Murray, J.F., Rao, B.D., Engan, K., Lee, T.W., Sejnowski, T.J. Dictionary learning algorithms for sparse representation. (2003) Computación neuronal. 15(2):349-96. DOI: 10.1162/089976603762552951

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Brandon, E.P., Lin, W., D'Amour, K.A., Pizzo, D.P., Dominguez, B., Sugiura, Y., Thode, S., Ko, C.P., Thal, L.J., Gage, F.H., Lee, K.F. Aberrant patterning of neuromuscular synapses in choline acetyltransferase-deficient mice. (2003) Diario de Neurociencia. 23(2):539-49.

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Rao, R.P., Sejnowski, T.J. Complex Cell-like Direction Selectivity through Spike-Timing Dependent Plasticity. (2003) IETE Journal of Research. 49(2-3):97-111.

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Lai, K., Kaspar, B.K., Gage, F.H., Schaffer, D.V. Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo. (2003) Nature Neuroscience. 6(1):21-7. DOI: 10.1038/nn983

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Kempermann, G., Gast, D., Kronenberg, G., Yamaguchi, M., Gage, F.H. Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice. (2003) Development. 130(2):391-9.

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Callaway, E.M. Orientation tuning--a crooked path to the straight and narrow. (2002) Neurona. 36(5):783-785.

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Suri, R.E., Sejnowski, T.J. Spike propagation synchronized by temporally asymmetric Hebbian learning. (2002) Biol Cybern. 87(5-6):440-5. DOI: 10.1007/s00422-002-0355-9

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Franks, K.M., Sejnowski, T.J. Complexity of calcium signaling in synaptic spines. (2002) BioEssays. 24(12):1130-1144. DOI: 10.1002/bies.10193

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Destexhe, A., Sejnowski, T.J. The initiation of bursts in thalamic neurons and the cortical control of thalamic sensitivity. (2002) Philos. Trans. R. Soc. Lond., B, Biol. Sci. 357(1428):1649-57. DOI: 10.1098/rstb.2002.1154

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Bartlett, M.S., Movellan, J.R., Sejnowski, T.J. Face recognition by independent component analysis. (2002) IEEE Trans Neural Netw. 13(6):1450-1464. DOI: 10.1109/TNN.2002.804287

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Franks, K.M., Bartol, T.M., Sejnowski, T.J. A Monte Carlo model reveals independent signaling at central glutamatergic synapses. (2002) Biophysical Journal. 83(5):2333-2348. DOI: 10.1016/S0006-3495(02)75248-X

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Bowd, C., Chan, K., Zangwill, L.M., Goldbaum, M.H., Lee, T.W., Sejnowski, T.J., Weinreb, R.N. Comparing neural networks and linear discriminant functions for glaucoma detection using confocal scanning laser ophthalmoscopy of the optic disc. (2002) Investigative Ophthalmology & Visual Science. 43(11):3444-3454.

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Horner, P.J., Gage, F.H. Regeneration in the adult and aging brain. (2002) Archives of Neurology. 59(11):1717-20.

Callaway, E.M., Yuste, R. Stimulating neurons with light. (2002) Current Opinion in Neurobiology. 12(5):587-592.

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Goulding, M., Lanuza, G., Sapir, T., Narayan, S. The formation of sensorimotor circuits. (2002) Current Opinion in Neurobiology. 12(5):508-15.

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Model of thalamocortical slow-wave sleep oscillations and transitions to activated States. (2002) Diario de Neurociencia. 22(19):8691-704.

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Zhao, X., Gage, F.H. Expressing the central nervous system. (2002) Neurochem. Res. 27(10):953-4.

Chatterjee, S., Callaway, E.M. S cone contributions to the magnocellular visual pathway in macaque monkey. (2002) Neurona. 35(6):1135-1146.

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Taupin, P., Gage, F.H. Adult neurogenesis and neural stem cells of the central nervous system in mammals. (2002) Journal of Neuroscience Research. 69(6):745-9. DOI: 10.1002/jnr.10378

Chan, K., Lee, T.W., Sample, P.A., Goldbaum, M.H., Weinreb, R.N., Sejnowski, T.J. Comparison of machine learning and traditional classifiers in glaucoma diagnosis. (2002) IEEE Trans Biomed Eng. 49(9):963-74. DOI: 10.1109/TBME.2002.802012

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Salinas, E., Sejnowski, T.J. Integrate-and-fire neurons driven by correlated stochastic input. (2002) Computación neuronal. 14(9):2111-2155. DOI: 10.1162/089976602320264024

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Tamaki, S., Eckert, K., He, D., Sutton, R., Doshe, M., Jain, G., Tushinski, R., Reitsma, M., Harris, B., Tsukamoto, A., Gage, F., Weissman, I., Uchida, N. Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. (2002) Journal of Neuroscience Research. 69(6):976-86. DOI: 10.1002/jnr.10412

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Direct isolation of human central nervous system stem cells (CNS-SC) based on cell surface markers yields a highly purified stem cell population that can extensively expand in vitro and exhibit multilineage differentiation potential both in vitro and in vivo. The CNS-SC were isolated from fetal brain tissue using the cell surface markers CD133(+), CD34(-), CD45(-), and CD24(-/lo) (CD133(+) cells). Fluorescence-activated cell sorted (FACS) CD133(+) cells continue to expand exponentially as neurospheres while retaining multipotential differentiation capacity for >10 passages. CD133(-), CD34(-), and CD45(-) sorted cells (approximately 95% of total fetal brain tissue) fail to initiate neurospheres. Neurosphere cells transplanted into neonatal immunodeficient NOD-SCID mice proliferated, migrated, and differentiated in a site-specific manner. However, it has been difficult to evaluate human cell engraftment, because many of the available monoclonal antibodies against neural cells (beta-tubulin III and glial fibrillary acidic protein) are not species specific. To trace the progeny of human cells after transplantation, CD133(+)-derived neurosphere cells were transduced with lentiviral vectors containing enhanced green fluorescent protein (eGFP) expressed downstream of the phosphoglycerate kinase promoter. After transduction, GFP(+) cells were enriched by FACS, expanded, and transplanted into the lateral ventricular space of neonatal immunodeficient NOD-SCID brain. The progeny of transplanted cells were detected by either GFP fluorescence or antibody against GFP. GFP(+) cells were present in the subventricular zone-rostral migrating stream, olfactory bulb, and hippocampus as well as nonneurogenic sites, such as cerebellum, cerebral cortex, and striatum. Antibody against GFP revealed that some of the cells displayed differentiating dendrites and processes with neurons or glia cells. Thus, marking human CNS-SC with reporter genes introduced by lentiviral vectors is a useful tool with which to characterize migration and differentiation of human cells in this mouse transplantation model.

Koo, S.J., Pfaff, S.L. Fine-tuning motor neuron properties: signaling from the periphery. (2002) Neurona. 35(5):823-6.

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Borrell, V., Callaway, E.M. Reorganization of exuberant axonal arbors contributes to the development of laminar specificity in ferret visual cortex. (2002) Diario de Neurociencia. 22(15):6682-6695. DOI: 20026663

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Lie, D.C., Dziewczapolski, G., Willhoite, A.R., Kaspar, B.K., Shults, C.W., Gage, F.H. The adult substantia nigra contains progenitor cells with neurogenic potential. (2002) Diario de Neurociencia. 22(15):6639-49. DOI: 20026700

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Chan, K., Lee, T.W., Sejnowski, T.J. Variational Learning of Clusters of Undercomplete Nonsymmetric Independent Components. (2002) J Mach Learn Res. 3:99-114.

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Hu, W.Y., Myers, C.P., Kilzer, J.M., Pfaff, S.L., Bushman, F.D. Inhibition of retroviral pathogenesis by RNA interference. (2002) Biología Actual. 12(15):1301-11.

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Lee, T.W., Wachtler, T., Sejnowski, T.J. Color opponency is an efficient representation of spectral properties in natural scenes. (2002) Vision Research. 42(17):2095-2103.

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Sample, P.A., Goldbaum, M.H., Chan, K., Boden, C., Lee, T.W., Vasile, C., Boehm, A.G., Sejnowski, T., Johnson, C.A., Weinreb, R.N. Using machine learning classifiers to identify glaucomatous change earlier in standard visual fields. (2002) Investigative Ophthalmology & Visual Science. 43(8):2660-2665.

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Kempermann, G., Gast, D., Gage, F.H. Neuroplasticity in old age: sustained fivefold induction of hippocampal neurogenesis by long-term environmental enrichment. (2002) Ann. Neurol. 52(2):135-43. DOI: 10.1002/ana.10262

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Thaler, J.P., Lee, S.K., Jurata, L.W., Gill, G.N., Pfaff, S.L. LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions. (2002) Móvil. 110(2):237-49.

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Houweling, A.R., Bazhenov, M., Timofeev, I., Grenier, F., Steriade, M., Sejnowski, T.J. Frequency-selective augmenting responses by short-term synaptic depression in cat neocortex. (2002) Journal of Physiology. 542(Pt 2):599-617.

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Lechner, H.A., Lein, E.S., Callaway, E.M. A genetic method for selective and quickly reversible silencing of Mammalian neurons. (2002) Diario de Neurociencia. 22(13):5287-5290. DOI: 20026527

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Tiesinga, P.H., Fellous, J.M., Sejnowski, T.J. Attractor reliability reveals deterministic structure in neuronal spike trains. (2002) Computación neuronal. 14(7):1629-1650. DOI: 10.1162/08997660260028647

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Timofeev, I., Grenier, F., Bazhenov, M., Houweling, A.R., Sejnowski, T.J., Steriade, M. Short- and medium-term plasticity associated with augmenting responses in cortical slabs and spindles in intact cortex of cats in vivo. (2002) Journal of Physiology. 542(Pt 2):583-598.

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Plastic changes in the synaptic responsiveness of neocortical neurones, which occur after rhythmic stimuli within the frequency range of sleep spindles (10 Hz), were investigated in isolated neocortical slabs and intact cortex of anaesthetized cats by means of single, dual and triple simultaneous intracellular recordings in conjunction with recordings of local field potential responses. In isolated cortical slabs (10 mm long, 6 mm wide and 4-5 mm deep), augmenting responses to pulse-trains at 10 Hz (responses with growing amplitudes from the second stimulus in a train) were elicited only by relatively high-intensity stimuli. At low intensities, responses were decremental. The largest augmenting responses were evoked in neurones located close to the stimulation site. Quantitative analyses of the number of action potentials and the amplitude and area of depolarization during augmenting responses in a population of neurones recorded from slabs showed that the most dramatic increases in the number of spikes with successive stimuli, and the greatest increase in depolarization amplitude, were found in conventional fast-spiking (FS) neurones. The largest increase in the area of depolarization was found in regular-spiking (RS) neurones. Dual intracellular recordings from a pair of FS and RS neurones in the slab revealed more action potentials in the FS neurone during augmenting responses and a significant increase in the depolarization area of the RS neurone that was dependent on the firing of the FS neurone. Self-sustained seizures could occur in the slab after rhythmic stimuli at 10 Hz. In the intact cortex, repeated sequences of stimuli generating augmenting responses or spontaneous spindles could induce an increased synaptic responsiveness to single stimuli, which lasted for several minutes. A similar time course of increased responsiveness was obtained with induction of cellular plasticity. These data suggest that augmenting responses elicited by stimulation, as well as spontaneously occurring spindles, may induce short- and medium-term plasticity of neuronal responses.

Timofeev, I., Bazhenov, M., Sejnowski, T., Steriade, M. Cortical hyperpolarization-activated depolarizing current takes part in the generation of focal paroxysmal activities. (2002) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 99(14):9533-9537. DOI: 10.1073/pnas.132259899

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Kempermann, G., Gage, F.H. Genetic determinants of adult hippocampal neurogenesis correlate with acquisition, but not probe trial performance, in the water maze task. (2002) European Journal of Neuroscience. 16(1):129-36.

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Eagleman, D.M., Sejnowski, T.J. Untangling spatial from temporal illusions. (2002) Trends in Neurosciences. 25(6):293; author reply 294.

Horner, P.J., Thallmair, M., Gage, F.H. Defining the NG2-expressing cell of the adult CNS. (2002) Journal of Neurocytology. 31(6-7):469-80.

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Gross, M.K., Dottori, M., Goulding, M. Lbx1 specifies somatosensory association interneurons in the dorsal spinal cord. (2002) Neurona. 34(4):535-49.

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Shirasaki, R., Pfaff, S.L. Transcriptional codes and the control of neuronal identity. (2002) Revisión Anual de Neurociencia. 25:251-81. DOI: 10.1146/annurev.neuro.25.112701.142916

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Song, H., Stevens, C.F., Gage, F.H. Astroglia induce neurogenesis from adult neural stem cells. (2002) Naturaleza. 417(6884):39-44. DOI: 10.1038/417039a

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Song, H.J., Stevens, C.F., Gage, F.H. Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons. (2002) Nature Neuroscience. 5(5):438-45. DOI: 10.1038/nn844

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Wiskott, L., Sejnowski, T.J. Slow feature analysis: unsupervised learning of invariances. (2002) Computación neuronal. 14(4):715-770. DOI: 10.1162/089976602317318938

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Duann, J.R., Jung, T.P., Kuo, W.J., Yeh, T.C., Makeig, S., Hsieh, J.C., Sejnowski, T.J. Single-trial variability in event-related BOLD signals. (2002) Neuroimage. 15(4):823-835. DOI: 10.1006/nimg.2001.1049

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Wurmser, A.E., Gage, F.H. Stem cells: cell fusion causes confusion. (2002) Naturaleza. 416(6880):485-7. DOI: 10.1038/416485a

Callaway, E.M. Cell type specificity of local cortical connections. (2002) Journal of Neurocytology. 31(3-5):231-237.

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Kempermann, G., Gage, F.H. Genetic influence on phenotypic differentiation in adult hippocampal neurogenesis. (2002) Brain Res. Dev. Brain Res. 134(1-2):1-12.

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van Praag, H., Gage, F.H. Genetics of childhood disorders: XXXVI. Stem cell research, part 1: New neurons in the adult brain. (2002) J Am Acad Child Adolesc Psychiatry. 41(3):354-6.

D'Amour, K.A., Gage, F.H. Are somatic stem cells pluripotent or lineage-restricted? (2002) Medicina Natural. 8(3):213-4. DOI: 10.1038/nm0302-213

Tiesinga, P.H., Fellous, J.M., José, J.V., Sejnowski, T.J. Information transfer in entrained cortical neurons. (2002) Network. 13(1):41-66.

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van Praag, H., Schinder, A.F., Christie, B.R., Toni, N., Palmer, T.D., Gage, F.H. Functional neurogenesis in the adult hippocampus. (2002) Naturaleza. 415(6875):1030-4. DOI: 10.1038/4151030a

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Kaspar, B.K., Vissel, B., Bengoechea, T., Crone, S., Randolph-Moore, L., Muller, R., Brandon, E.P., Schaffer, D., Verma, I.M., Lee, K.F., Heinemann, S.F., Gage, F.H. Adeno-associated virus effectively mediates conditional gene modification in the brain. (2002) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 99(4):2320-5. DOI: 10.1073/pnas.042678699

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Gage, F.H. Neurogenesis in the adult brain. (2002) Diario de Neurociencia. 22(3):612-3.

Makeig, S., Westerfield, M., Jung, T.P., Enghoff, S., Townsend, J., Courchesne, E., Sejnowski, T.J. Dynamic brain sources of visual evoked responses. (2002) Ciencia. 295(5555):690-4. DOI: 10.1126/science.1066168

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Kaspar, B.K., Erickson, D., Schaffer, D., Hinh, L., Gage, F.H., Peterson, D.A. Targeted retrograde gene delivery for neuronal protection. (2002) Mol. Ther. 5(1):50-6. DOI: 10.1006/mthe.2001.0520

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Vallières, L., Campbell, I.L., Gage, F.H., Sawchenko, P.E. Reduced hippocampal neurogenesis in adult transgenic mice with chronic astrocytic production of interleukin-6. (2002) Diario de Neurociencia. 22(2):486-92.

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Goldbaum, M.H., Sample, P.A., Chan, K., Williams, J., Lee, T.W., Blumenthal, E., Girkin, C.A., Zangwill, L.M., Bowd, C., Sejnowski, T., Weinreb, R.N. Comparing machine learning classifiers for diagnosing glaucoma from standard automated perimetry. (2002) Investigative Ophthalmology & Visual Science. 43(1):162-9.

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Zhao, X., Lein, E.S., He, A., Smith, S.C., Aston, C., Gage, F.H. Transcriptional profiling reveals strict boundaries between hippocampal subregions. (2001) Journal of Comparative Neurology. 441(3):187-96.

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Flash, T., Sejnowski, T.J. Computational approaches to motor control. (2001) Current Opinion in Neurobiology. 11(6):655-62.

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Callaway, E.M. Neural mechanisms for the generation of visual complex cells. (2001) Neurona. 32(3):378-380.

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Lee, S.K., Pfaff, S.L. Transcriptional networks regulating neuronal identity in the developing spinal cord. (2001) Nature Neuroscience. 4 Suppl:1183-91. DOI: 10.1038/nn750

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Dottori, M., Gross, M.K., Labosky, P., Goulding, M. The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. (2001) Development. 128(21):4127-38.

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Destexhe, A., Rudolph, M., Fellous, J.M., Sejnowski, T.J. Fluctuating synaptic conductances recreate in vivo-like activity in neocortical neurons. (2001) Neuroscience. 107(1):13-24.

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Jung, T.P., Makeig, S., Westerfield, M., Townsend, J., Courchesne, E., Sejnowski, T.J. Analysis and visualization of single-trial event-related potentials. (2001) Hum Brain Mapp. 14(3):166-85.

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In this study, a linear decomposition technique, independent component analysis (ICA), is applied to single-trial multichannel EEG data from event-related potential (ERP) experiments. Spatial filters derived by ICA blindly separate the input data into a sum of temporally independent and spatially fixed components arising from distinct or overlapping brain or extra-brain sources. Both the data and their decomposition are displayed using a new visualization tool, the "ERP image," that can clearly characterize single-trial variations in the amplitudes and latencies of evoked responses, particularly when sorted by a relevant behavioral or physiological variable. These tools were used to analyze data from a visual selective attention experiment on 28 control subjects plus 22 neurological patients whose EEG records were heavily contaminated with blink and other eye-movement artifacts. Results show that ICA can separate artifactual, stimulus-locked, response-locked, and non-event-related background EEG activities into separate components, a taxonomy not obtained from conventional signal averaging approaches. This method allows: (1) removal of pervasive artifacts of all types from single-trial EEG records, (2) identification and segregation of stimulus- and response-locked EEG components, (3) examination of differences in single-trial responses, and (4) separation of temporally distinct but spatially overlapping EEG oscillatory activities with distinct relationships to task events. The proposed methods also allow the interaction between ERPs and the ongoing EEG to be investigated directly. We studied the between-subject component stability of ICA decomposition of single-trial EEG epochs by clustering components with similar scalp maps and activation power spectra. Components accounting for blinks, eye movements, temporal muscle activity, event-related potentials, and event-modulated alpha activities were largely replicated across subjects. Applying ICA and ERP image visualization to the analysis of sets of single trials from event-related EEG (or MEG) experiments can increase the information available from ERP (or ERF) data.

Salinas, E., Sejnowski, T.J. Gain modulation in the central nervous system: where behavior, neurophysiology, and computation meet. (2001) The Neuroscientist. 7(5):430-40. DOI: 10.1177/107385840100700512

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Marquardt, T., Pfaff, S.L. Cracking the transcriptional code for cell specification in the neural tube. (2001) Móvil. 106(6):651-4.

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Pfeifer, A., Brandon, E.P., Kootstra, N., Gage, F.H., Verma, I.M. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. (2001) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 98(20):11450-5. DOI: 10.1073/pnas.201415498

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Rao, R.P., Sejnowski, T.J. Predictive learning of temporal sequences in recurrent neocortical circuits. (2001) Novartis Found. Symp. 239:208-229; discussion 229-240.

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Salinas, E., Sejnowski, T.J. Correlated neuronal activity and the flow of neural information. (2001) Nature Reviews Neuroscience. 2(8):539-50. DOI: 10.1038/35086012

Chu, V.T., Gage, F.H. Chipping away at stem cells. (2001) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 98(14):7652-3. DOI: 10.1073/pnas.141244198

Jung, T.P., Makeig, S., McKeown, M.J., Bell, A.J., Lee, T.W., Sejnowski, T.J. Imaging Brain Dynamics Using Independent Component Analysis. (2001) Proceedings of the IEEE. Institute of Electrical and Electronics Engineers. 89(7):1107-1122. DOI: 10.1109/5.939827

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Pouget, A., Sejnowski, T.J. Simulating a lesion in a basis function model of spatial representations: comparison with hemineglect. (2001) Psychol Rev. 108(3):653-73.

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Läer, L., Kloppstech, M., Schöfl, C., Sejnowski, T.J., Brabant, G., Prank, K. Noise enhanced hormonal signal transduction through intracellular calcium oscillations. (2001) Biophysical Chemistry. 91(2):157-66.

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Tiesinga, P.H., Fellous, J.M., José, J.V., Sejnowski, T.J. Computational model of carbachol-induced delta, theta, and gamma oscillations in the hippocampus. (2001) Hipocampo. 11(3):251-274. DOI: 10.1002/hipo.1041

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Zhang, K., Sejnowski, T.J. Accuracy and learning in neuronal populations. (2001) Progress in Brain Research. 130:333-342.

van Praag, H., Barlow, C., Gage, F.H. Are drug targets missed owing to lack of physical activity? - Reply. (2001) Drug Discov. Today. 6(12):615-617.

Ray, J., Gage, F.H. Construction of cells expressing neurotrophins. (2001) Métodos en Biología Molecular. 169:115-33.

Matsuura, N., Lie, D.C., Hoshimaru, M., Asahi, M., Hojo, M., Ishizaki, R., Hashimoto, N., Noji, S., Ohuchi, H., Yoshioka, H., Gage, F.H. Sonic hedgehog facilitates dopamine differentiation in the presence of a mesencephalic glial cell line. (2001) Diario de Neurociencia. 21(12):4326-35.

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Rao, R.P., Eagleman, D.M., Sejnowski, T.J. Optimal smoothing in visual motion perception. (2001) Computación neuronal. 13(6):1243-53.

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Nicholson, L.F., Ma, L., Goulding, M. Cloning and expression of Munc 30: a member of the paired -like homeodomain gene family. (2001) Cell Biology International. 25(4):351-65. DOI: 10.1006/cbir.2000.0638

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Tiesinga, P.H., Sejnowski, T.J. Precision of pulse-coupled networks of integrate-and-fire neurons. (2001) Network. 12(2):215-33.

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Bazhenov, M., Stopfer, M., Rabinovich, M., Abarbanel, H.D., Sejnowski, T.J., Laurent, G. Model of cellular and network mechanisms for odor-evoked temporal patterning in the locust antennal lobe. (2001) Neurona. 30(2):569-81.

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Bazhenov, M., Stopfer, M., Rabinovich, M., Huerta, R., Abarbanel, H.D., Sejnowski, T.J., Laurent, G. Model of transient oscillatory synchronization in the locust antennal lobe. (2001) Neurona. 30(2):553-567.

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Briggs, F., Callaway, E.M. Layer-specific input to distinct cell types in layer 6 of monkey primary visual cortex. (2001) Diario de Neurociencia. 21(10):3600-3608.

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Wachtler, T., Albright, T.D., Sejnowski, T.J. Nonlocal interactions in color perception: nonlinear processing of chromatic signals from remote inducers. (2001) Vision Research. 41(12):1535-1546.

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Palmer, T.D., Schwartz, P.H., Taupin, P., Kaspar, B., Stein, S.A., Gage, F.H. Cell culture. Progenitor cells from human brain after death. (2001) Naturaleza. 411(6833):42-3. DOI: 10.1038/35075141

Simon, H.H., Saueressig, H., Wurst, W., Goulding, M.D., O'Leary, D.D. Fate of midbrain dopaminergic neurons controlled by the engrailed genes. (2001) Diario de Neurociencia. 21(9):3126-34.

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Lin, W., Burgess, R.W., Dominguez, B., Pfaff, S.L., Sanes, J.R., Lee, K.F. Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse. (2001) Naturaleza. 410(6832):1057-64. DOI: 10.1038/35074025

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Suhr, S.T., Senut, M.C., Whitelegge, J.P., Faull, K.F., Cuizon, D.B., Gage, F.H. Identities of sequestered proteins in aggregates from cells with induced polyglutamine expression. (2001) Revista de Biología Celular. 153(2):283-94.

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Yabuta, N.H., Sawatari, A., Callaway, E.M. Two functional channels from primary visual cortex to dorsal visual cortical areas. (2001) Ciencia. 292(5515):297-300. DOI: 10.1126/science.1057916

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Fellous, J.M., Houweling, A.R., Modi, R.H., Rao, R.P., Tiesinga, P.H., Sejnowski, T.J. Frequency dependence of spike timing reliability in cortical pyramidal cells and interneurons. (2001) Journal of Neurophysiology. 85(4):1782-1787.

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Lamar, E., Kintner, C., Goulding, M. Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation. (2001) Development. 128(8):1335-46.

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Anderson, D.J., Gage, F.H., Weissman, I.L. Can stem cells cross lineage boundaries? (2001) Medicina Natural. 7(4):393-5. DOI: 10.1038/86439

Allen, D.M., van Praag, H., Ray, J., Weaver, Z., Winrow, C.J., Carter, T.A., Braquet, R., Harrington, E., Ried, T., Brown, K.D., Gage, F.H., Barlow, C. Ataxia telangiectasia mutated is essential during adult neurogenesis. (2001) Genes y Desarrollo. 15(5):554-66. DOI: 10.1101/gad.869001

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Dykman, M.I., Sharpee, T., Platzman, P.M. Enhancement of tunneling from a correlated 2D electron system by a many-electron Mössbauer-type recoil in a magnetic field. (2001) Phys. Rev. Lett. 86(11):2408-11. DOI: 10.1103/PhysRevLett.86.2408

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Weissman, I.L., Anderson, D.J., Gage, F.H. Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. (2001) Annual Review of Cell and Developmental Biology. 17:387-403. DOI: 10.1146/annurev.cellbio.17.1.387

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Butler, A.K., Dantzker, J.L., Shah, R.B., Callaway, E.M. Development of visual cortical axons: layer-specific effects of extrinsic influences and activity blockade. (2001) Journal of Comparative Neurology. 430(3):321-331.

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Moran-Rivard, L., Kagawa, T., Saueressig, H., Gross, M.K., Burrill, J., Goulding, M. Evx1 is a postmitotic determinant of v0 interneuron identity in the spinal cord. (2001) Neurona. 29(2):385-99.

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Pierani, A., Moran-Rivard, L., Sunshine, M.J., Littman, D.R., Goulding, M., Jessell, T.M. Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. (2001) Neurona. 29(2):367-384.

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Vissel, B., Royle, G.A., Christie, B.R., Schiffer, H.H., Ghetti, A., Tritto, T., Perez-Otano, I., Radcliffe, R.A., Seamans, J., Sejnowski, T., Wehner, J.M., Collins, A.C., O'Gorman, S., Heinemann, S.F. The role of RNA editing of kainate receptors in synaptic plasticity and seizures. (2001) Neurona. 29(1):217-227.

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Brown, G.D., Yamada, S., Sejnowski, T.J. Independent component analysis at the neural cocktail party. (2001) Trends in Neurosciences. 24(1):54-63.

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Wachtler, T., Lee, T.W., Sejnowski, T.J. Chromatic structure of natural scenes. (2001) J Opt Soc Am A Opt Image Sci Vis. 18(1):65-77.

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Seamans, J.K., Durstewitz, D., Christie, B.R., Stevens, C.F., Sejnowski, T.J. Dopamine D1/D5 receptor modulation of excitatory synaptic inputs to layer V prefrontal cortex neurons. (2001) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 98(1):301-306. DOI: 10.1073/pnas.011518798

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van Praag, H., Kempermann, G., Gage, F.H. Neural consequences of environmental enrichment. (2000) Nature Reviews Neuroscience. 1(3):191-8. DOI: 10.1038/35044558

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Kempermann, G., van Praag, H., Gage, F.H. Activity-dependent regulation of neuronal plasticity and self repair. (2000) Progress in Brain Research. 127:35-48.

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Tiesinga, P.H., José, J.V., Sejnowski, T.J. Comparison of current-driven and conductance-driven neocortical model neurons with Hodgkin-Huxley voltage-gated channels. (2000) Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 62(6 Pt B):8413-8419.

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Uchida, N., Buck, D.W., He, D., Reitsma, M.J., Masek, M., Phan, T.V., Tsukamoto, A.S., Gage, F.H., Weissman, I.L. Direct isolation of human central nervous system stem cells. (2000) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 97(26):14720-5. DOI: 10.1073/pnas.97.26.14720

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Sejnowski, T.J., Destexhe, A. Why do we sleep? (2000) Brain Research. 886(1-2):208-223.

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Shihabuddin, L.S., Horner, P.J., Ray, J., Gage, F.H. Adult spinal cord stem cells generate neurons after transplantation in the adult dentate gyrus. (2000) Diario de Neurociencia. 20(23):8727-35.

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Makeig, S., Jung, T.P., Sejnowski, T.J. Awareness during drowsiness: dynamics and electrophysiological correlates. (2000) Can J Exp Psychol. 54(4):266-273.

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Christie, B.R., Franks, K.M., Seamans, J.K., Saga, K., Sejnowski, T.J. Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells. (2000) Hipocampo. 10(6):673-683. DOI: 10.1002/1098-1063(2000)10:6<673::aid-hipo1005>3.0.

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Timofeev, I., Grenier, F., Bazhenov, M., Sejnowski, T.J., Steriade, M. Origin of slow cortical oscillations in deafferented cortical slabs. (2000) Cerebral Cortex . 10(12):1185-1199.

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Taupin, P., Ray, J., Fischer, W.H., Suhr, S.T., Hakansson, K., Grubb, A., Gage, F.H. FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor. (2000) Neurona. 28(2):385-97.

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Durstewitz, D., Seamans, J.K., Sejnowski, T.J. Neurocomputational models of working memory. (2000) Nature Neuroscience. 3 Suppl:1184-91. DOI: 10.1038/81460

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Shults, C.W., Ray, J., Tsuboi, K., Gage, F.H. Fibroblast growth factor-2-producing fibroblasts protect the nigrostriatal dopaminergic system from 6-hydroxydopamine. (2000) Brain Research. 883(2):192-204.

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We tested the hypothesis that fibroblasts, which had been genetically engineered to produce fibroblast growth factor-2 (FGF-2), can protect nigrostriatal dopaminergic neurons. Three groups of rats received either a burr hole only (n=5) or implantation of fibroblasts, which had been genetically engineered to produce beta-galactosidase (beta-gal) (n=8) or FGF-2 (n=8), at two sites in the right striatum. Two weeks later, the animals received an injection of 25 microg of 6-hydroxydopamine hydrobromide (6-OHDA) midway between the two implant sites. The group that received FGF-2-fibroblasts had significantly fewer apomorphine-induced rotations than the groups that received a burr hole only or beta-gal-fibroblasts at weeks 2 and 3 following lesioning with 6-OHDA. Testing for amphetamine-induced rotation revealed a mild reduction in rotation in the beta-gal-fibroblast group compared to the burr hole only group, but a striking attenuation of amphetamine-induced rotation in the FGF-2-fibroblast group. There was also preservation of TH-IR neurons on the lesioned side relative to both control groups. The size of the grafts and the gliosis surrounding the injection sites did not differ between the FGF-2-fibroblast and beta-gal-fibroblast groups. To further characterize the production of FGF-2 by the FGF-2-fibroblasts, we implanted FGF-2-fibroblasts and beta-gal-fibroblast into the striatum of rats but did not lesion the animals with 6-OHDA. The animals were then sacrificed at 1, 2 and 5 weeks following implantation. Prior to implantation the FGF-2 fibroblasts contained 148 ng/mg of FGF-2-immunoreactive (FGF-2-IR) material per mg of protein of cell lysate. After implantation FGF-2-IR material was noted in the grafts of FGF-2-fibroblasts, most conspicuously at 1 and 2 weeks following implantation. We also noted FGF-2-IR material in the nuclei of reactive astrocytes adjacent to the implants, and OX-42-immunoreactive (OX-42-IR) cells adjacent and occasionally within the implants. Our work indicates that fibroblasts genetically engineered to produce FGF-2 and implanted in the striatum can protect the nigrostriatal dopaminergic system and may be useful in the treatment of Parkinson's disease.

Horner, P.J., Gage, F.H. Regenerating the damaged central nervous system. (2000) Naturaleza. 407(6807):963-70. DOI: 10.1038/35039559

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Zoghbi, H.Y., Gage, F.H., Choi, D.W. Neurobiology of disease. (2000) Current Opinion in Neurobiology. 10(5):655-60.

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Gage, F.H. Structural plasticity: cause, result, or correlate of depression. (2000) Psiquiatría Biológica. 48(8):713-4.

Palmer, T.D., Willhoite, A.R., Gage, F.H. Vascular niche for adult hippocampal neurogenesis. (2000) Journal of Comparative Neurology. 425(4):479-94.

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Jung, T.P., Makeig, S., Westerfield, M., Townsend, J., Courchesne, E., Sejnowski, T.J. Removal of eye activity artifacts from visual event-related potentials in normal and clinical subjects. (2000) Clin Neurophysiol. 111(10):1745-58.

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Young, M.J., Ray, J., Whiteley, S.J., Klassen, H., Gage, F.H. Neuronal differentiation and morphological integration of hippocampal progenitor cells transplanted to the retina of immature and mature dystrophic rats. (2000) Molecular and Cellular Neurosciences. 16(3):197-205. DOI: 10.1006/mcne.2000.0869

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Sharma, K., Leonard, A.E., Lettieri, K., Pfaff, S.L. Genetic and epigenetic mechanisms contribute to motor neuron pathfinding. (2000) Naturaleza. 406(6795):515-9. DOI: 10.1038/35020078

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T. Spiking-bursting activity in the thalamic reticular nucleus initiates sequences of spindle oscillations in thalamic networks. (2000) Journal of Neurophysiology. 84(2):1076-87.

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Salinas, E., Sejnowski, T.J. Impact of correlated synaptic input on output firing rate and variability in simple neuronal models. (2000) Diario de Neurociencia. 20(16):6193-209.

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Yabuta, N.H., Butler, A.K., Callaway, E.M. Laminar specificity of local circuits in barrel cortex of ephrin-A5 knockout mice. (2000) Diario de Neurociencia. 20(15):RC88.

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Krekelberg, B., Lappe, M., Whitney, D., Cavanagh, P., Eagleman, D.M., Sejnowski, T.J. The position of moving objects. (2000) Ciencia. 289(5482):1107a. DOI: 10.1126/science.289.5482.1107a

Verma, I.M., Gage, F.H. Genome and gene therapy. (2000) Molecular Therapy. 2(2):95. DOI: 10.1006/mthe.2000.0109

Goulding, M., Lamar, E. Neuronal patterning: Making stripes in the spinal cord. (2000) Biología Actual. 10(15):565-568.

Brown, A., Yates, P.A., Burrola, P., Ortuño, D., Vaidya, A., Jessell, T.M., Pfaff, S.L., O'Leary, D.D., Lemke, G. Topographic mapping from the retina to the midbrain is controlled by relative but not absolute levels of EphA receptor signaling. (2000) Móvil. 102(1):77-88.

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Mah, S.P., Saueressig, H., Goulding, M., Kintner, C., Dressler, G.R. Kidney development in cadherin-6 mutants: delayed mesenchyme-to-epithelial conversion and loss of nephrons. (2000) Developmental Biology. 223(1):38-53. DOI: 10.1006/dbio.2000.9738

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Dantzker, J.L., Callaway, E.M. Laminar sources of synaptic input to cortical inhibitory interneurons and pyramidal neurons. (2000) Nature Neuroscience. 3(7):701-707. DOI: 10.1038/76656

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Kempermann, G., Gage, F.H. Neurogenesis in the adult hippocampus. (2000) Novartis Found. Symp. 231:220-35; discussion 235-41, 302.

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Kafri, T., van Praag, H., Gage, F.H., Verma, I.M. Lentiviral vectors: regulated gene expression. (2000) Molecular Therapy. 1(6):516-21. DOI: 10.1006/mthe.2000.0083

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Makeig, S., Enghoff, S., Jung, T.P., Sejnowski, T.J. A natural basis for efficient brain-actuated control. (2000) IEEE Trans Rehabil Eng. 8(2):208-11.

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Zhang, K., Sejnowski, T.J. A universal scaling law between gray matter and white matter of cerebral cortex. (2000) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 97(10):5621-6. DOI: 10.1073/pnas.090504197

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Jacobs, B.L., van Praag, H., Gage, F.H. Adult brain neurogenesis and psychiatry: a novel theory of depression. (2000) Psiquiatría Molecular. 5(3):262-9.

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D'Amour, K., Gage, F.H. New tools for human developmental biology. (2000) Nature Biotechnology. 18(4):381-2. DOI: 10.1038/74429

Paulsen, O., Sejnowski, T.J. Natural patterns of activity and long-term synaptic plasticity. (2000) Current Opinion in Neurobiology. 10(2):172-9.

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Fellous, J.M., Sejnowski, T.J. Cholinergic induction of oscillations in the hippocampal slice in the slow (0.5-2 Hz), theta (5-12 Hz), and gamma (35-70 Hz) bands. (2000) Hipocampo. 10(2):187-97. DOI: 10.1002/(SICI)1098-1063(2000)10:2<187::aid-hipo8>3

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Jung, T.P., Makeig, S., Humphries, C., Lee, T.W., McKeown, M.J., Iragui, V., Sejnowski, T.J. Removing electroencephalographic artifacts by blind source separation. (2000) Psychophysiology. 37(2):163-78.

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Eagleman, D.M., Sejnowski, T.J. Motion integration and postdiction in visual awareness. (2000) Ciencia. 287(5460):2036-8.

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Durstewitz, D., Seamans, J.K., Sejnowski, T.J. Dopamine-mediated stabilization of delay-period activity in a network model of prefrontal cortex. (2000) Journal of Neurophysiology. 83(3):1733-50.

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The prefrontal cortex (PFC) is critically involved in working memory, which underlies memory-guided, goal-directed behavior. During working-memory tasks, PFC neurons exhibit sustained elevated activity, which may reflect the active holding of goal-related information or the preparation of forthcoming actions. Dopamine via the D1 receptor strongly modulates both this sustained (delay-period) activity and behavioral performance in working-memory tasks. However, the function of dopamine during delay-period activity and the underlying neural mechanisms are only poorly understood. Recently we proposed that dopamine might stabilize active neural representations in PFC circuits during tasks involving working memory and render them robust against interfering stimuli and noise. To further test this idea and to examine the dopamine-modulated ionic currents that could give rise to increased stability of neural representations, we developed a network model of the PFC consisting of multicompartment neurons equipped with Hodgkin-Huxley-like channel kinetics that could reproduce in vitro whole cell and in vivo recordings from PFC neurons. Dopaminergic effects on intrinsic ionic and synaptic conductances were implemented in the model based on in vitro data. Simulated dopamine strongly enhanced high, delay-type activity but not low, spontaneous activity in the model network. Furthermore the strength of an afferent stimulation needed to disrupt delay-type activity increased with the magnitude of the dopamine-induced shifts in network parameters, making the currently active representation much more stable. Stability could be increased by dopamine-induced enhancements of the persistent Na(+) and N-methyl-D-aspartate (NMDA) conductances. Stability also was enhanced by a reduction in AMPA conductances. The increase in GABA(A) conductances that occurs after stimulation of dopaminergic D1 receptors was necessary in this context to prevent uncontrolled, spontaneous switches into high-activity states (i.e., spontaneous activation of task-irrelevant representations). In conclusion, the dopamine-induced changes in the biophysical properties of intrinsic ionic and synaptic conductances conjointly acted to highly increase stability of activated representations in PFC networks and at the same time retain control over network behavior and thus preserve its ability to adequately respond to task-related stimuli. Predictions of the model can be tested in vivo by locally applying specific D1 receptor, NMDA, or GABA(A) antagonists while recording from PFC neurons in delayed reaction-type tasks with interfering stimuli.

Horner, P.J., Power, A.E., Kempermann, G., Kuhn, H.G., Palmer, T.D., Winkler, J., Thal, L.J., Gage, F.H. Proliferation and differentiation of progenitor cells throughout the intact adult rat spinal cord. (2000) Diario de Neurociencia. 20(6):2218-28.

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Barabash-Sharpee, T., Dykman, M.I., Platzman, P.M. Tunneling transverse to a magnetic field and its occurrence in correlated 2D electron systems. (2000) Phys. Rev. Lett. 84(10):2227-30. DOI: 10.1103/PhysRevLett.84.2227

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Sawatari, A., Callaway, E.M. Diversity and cell type specificity of local excitatory connections to neurons in layer 3B of monkey primary visual cortex. (2000) Neurona. 25(2):459-471.

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Gage, F.H. Mammalian neural stem cells. (2000) Ciencia. 287(5457):1433-8.

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Jurata, L.W., Thomas, J.B., Pfaff, S.L. Transcriptional mechanisms in the development of motor control. (2000) Current Opinion in Neurobiology. 10(1):72-9.

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Moortgat, K.T., Bullock, T.H., Sejnowski, T.J. Gap junction effects on precision and frequency of a model pacemaker network. (2000) Journal of Neurophysiology. 83(2):984-97.

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Moortgat, K.T., Bullock, T.H., Sejnowski, T.J. Precision of the pacemaker nucleus in a weakly electric fish: network versus cellular influences. (2000) Journal of Neurophysiology. 83(2):971-83.

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We investigated the relative influence of cellular and network properties on the extreme spike timing precision observed in the medullary pacemaker nucleus (Pn) of the weakly electric fish Apteronotus leptorhynchus. Of all known biological rhythms, the electric organ discharge of this and related species is the most temporally precise, with a coefficient of variation (CV = standard deviation/mean period) of 2 x 10(-4) and standard deviation (SD) of 0.12-1.0 micros. The timing of the electric organ discharge is commanded by neurons of the Pn, individual cells of which we show in an in vitro preparation to have only a slightly lesser degree of precision. Among the 100-150 Pn neurons, dye injection into a pacemaker cell resulted in dye coupling in one to five other pacemaker cells and one to three relay cells, consistent with previous results. Relay cell fills, however, showed profuse dendrites and contacts never seen before: relay cell dendrites dye-coupled to one to seven pacemaker and one to seven relay cells. Moderate (0.1-10 nA) intracellular current injection had no effect on a neuron's spiking period, and only slightly modulated its spike amplitude, but could reset the spike phase. In contrast, massive hyperpolarizing current injections (15-25 nA) could force the cell to skip spikes. The relative timing of subthreshold and full spikes suggested that at least some pacemaker cells are likely to be intrinsic oscillators. The relative amplitudes of the subthreshold and full spikes gave a lower bound to the gap junctional coupling coefficient of 0.01-0.08. Three drugs, called gap junction blockers for their mode of action in other preparations, caused immediate and substantial reduction in frequency, altered the phase lag between pairs of neurons, and later caused the spike amplitude to drop, without altering the spike timing precision. Thus we conclude that the high precision of the normal Pn rhythm does not require maximal gap junction conductances between neurons that have ordinary cellular precision. Rather, the spiking precision can be explained as an intrinsic cellular property while the gap junctions act to frequency- and phase-lock the network oscillations.

Lewicki, M.S., Sejnowski, T.J. Learning overcomplete representations. (2000) Computación neuronal. 12(2):337-65.

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Senut, M.C., Suhr, S.T., Kaspar, B., Gage, F.H. Intraneuronal aggregate formation and cell death after viral expression of expanded polyglutamine tracts in the adult rat brain. (2000) Diario de Neurociencia. 20(1):219-29.

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Gross, M.K., Moran-Rivard, L., Velasquez, T., Nakatsu, M.N., Jagla, K., Goulding, M. Lbx1 is required for muscle precursor migration along a lateral pathway into the limb. (2000) Development. 127(2):413-424.

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Murthy, V.N., Sejnowski, T.J., Stevens, C.F. Dynamics of dendritic calcium transients evoked by quantal release at excitatory hippocampal synapses. (2000) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 97(2):901-6.

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Sejnowski, T.J. The book of Hebb. (1999) Neurona. 24(4):773-6.

Spain, B.H., Larson, C.J., Shihabuddin, L.S., Gage, F.H., Verma, I.M. Truncated BRCA2 is cytoplasmic: implications for cancer-linked mutations. (1999) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 96(24):13920-5.

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van Praag, H., Christie, B.R., Sejnowski, T.J., Gage, F.H. Running enhances neurogenesis, learning, and long-term potentiation in mice. (1999) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 96(23):13427-31.

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Shihabuddin, L.S., Palmer, T.D., Gage, F.H. The search for neural progenitor cells: prospects for the therapy of neurodegenerative disease. (1999) Mol Med Today. 5(11):474-80.

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McKeown, M.J., Humphries, C., Iragui, V., Sejnowski, T.J. Spatially fixed patterns account for the spike and wave features in absence seizures. (1999) Brain Topogr. 12(2):107-16.

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Sailer, A., Swanson, G.T., Pérez-Otaño, I., O'Leary, L., Malkmus, S.A., Dyck, R.H., Dickinson-Anson, H., Schiffer, H.H., Maron, C., Yaksh, T.L., Gage, F.H., O'Gorman, S., Heinemann, S.F. Generation and analysis of GluR5(Q636R) kainate receptor mutant mice. (1999) Diario de Neurociencia. 19(20):8757-64.

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Palmer, T.D., Markakis, E.A., Willhoite, A.R., Safar, F., Gage, F.H. Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS. (1999) Diario de Neurociencia. 19(19):8487-97.

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Saueressig, H., Burrill, J., Goulding, M. Engrailed-1 and netrin-1 regulate axon pathfinding by association interneurons that project to motor neurons. (1999) Development. 126(19):4201-4212.

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Donato, G., Bartlett, M.S., Hager, J.C., Ekman, P., Sejnowski, T.J. Classifying Facial Actions. (1999) IEEE Trans Pattern Anal Mach Intell. 21(10):974. DOI: 10.1109/34.799905

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Sakurada, K., Ohshima-Sakurada, M., Palmer, T.D., Gage, F.H. Nurr1, an orphan nuclear receptor, is a transcriptional activator of endogenous tyrosine hydroxylase in neural progenitor cells derived from the adult brain. (1999) Development. 126(18):4017-26.

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Takahashi, M., Miyoshi, H., Verma, I.M., Gage, F.H. Rescue from photoreceptor degeneration in the rd mouse by human immunodeficiency virus vector-mediated gene transfer. (1999) Journal of Virology. 73(9):7812-6.

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Smith, D.E., Roberts, J., Gage, F.H., Tuszynski, M.H. Age-associated neuronal atrophy occurs in the primate brain and is reversible by growth factor gene therapy. (1999) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 96(19):10893-8.

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Harrison, K.A., Thaler, J., Pfaff, S.L., Gu, H., Kehrl, J.H. Pancreas dorsal lobe agenesis and abnormal islets of Langerhans in Hlxb9-deficient mice. (1999) Nature Genetics. 23(1):71-5. DOI: 10.1038/12674

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Thaler, J., Harrison, K., Sharma, K., Lettieri, K., Kehrl, J., Pfaff, S.L. Active suppression of interneuron programs within developing motor neurons revealed by analysis of homeodomain factor HB9. (1999) Neurona. 23(4):675-87.

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Bang, A.G., Papalopulu, N., Goulding, M.D., Kintner, C. Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-mediated signal from posterior nonaxial mesoderm. (1999) Developmental Biology. 212(2):366-80. DOI: 10.1006/dbio.1999.9319

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Lehky, S.R., Sejnowski, T.J. Seeing white: Qualia in the context of decoding population codes. (1999) Computación neuronal. 11(6):1261-80.

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Makeig, S., Westerfield, M., Townsend, J., Jung, T.P., Courchesne, E., Sejnowski, T.J. Functionally independent components of early event-related potentials in a visual spatial attention task. (1999) Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354(1387):1135-44. DOI: 10.1098/rstb.1999.0469

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Kempermann, G., Gage, F.H. Experience-dependent regulation of adult hippocampal neurogenesis: effects of long-term stimulation and stimulus withdrawal. (1999) Hipocampo. 9(3):321-32. DOI: 10.1002/(SICI)1098-1063(1999)9:3<321::aid-hipo11>3

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Kempermann, G., Gage, F.H. New nerve cells for the adult brain. (1999) Sci. Am. 280(5):48-53.

Steward, O., Schauwecker, P.E., Guth, L., Zhang, Z., Fujiki, M., Inman, D., Wrathall, J., Kempermann, G., Gage, F.H., Saatman, K.E., Raghupathi, R., McIntosh, T. Genetic approaches to neurotrauma research: opportunities and potential pitfalls of murine models. (1999) Experimental Neurology. 157(1):19-42. DOI: 10.1006/exnr.1999.7040

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Senut, M.C., Gage, F.H. Prenatal gene therapy: can the technical hurdles be overcome? (1999) Mol Med Today. 5(4):152-6.

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Markakis, E.A., Gage, F.H. Adult-generated neurons in the dentate gyrus send axonal projections to field CA3 and are surrounded by synaptic vesicles. (1999) Journal of Comparative Neurology. 406(4):449-60.

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Zhang, K., Sejnowski, T.J. A theory of geometric constraints on neural activity for natural three-dimensional movement. (1999) Diario de Neurociencia. 19(8):3122-45.

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Ueba, T., Kaspar, B., Zhao, X., Gage, F.H. Repression of human fibroblast growth factor 2 by a novel transcription factor. (1999) Revista de Química Biológica. 274(15):10382-7.

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Makeig, S., Westerfield, M., Jung, T.P., Covington, J., Townsend, J., Sejnowski, T.J., Courchesne, E. Functionally independent components of the late positive event-related potential during visual spatial attention. (1999) Diario de Neurociencia. 19(7):2665-80.

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van Praag, H., Kempermann, G., Gage, F.H. Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus. (1999) Nature Neuroscience. 2(3):266-70. DOI: 10.1038/6368

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Suhr, S.T., Gage, F.H. Gene therapy in the central nervous system: the use of recombinant retroviruses. (1999) Archives of Neurology. 56(3):287-92.

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Bartlett, M.S., Hager, J.C., Ekman, P., Sejnowski, T.J. Measuring facial expressions by computer image analysis. (1999) Psychophysiology. 36(2):253-63.

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Self-sustained rhythmic activity in the thalamic reticular nucleus mediated by depolarizing GABAA receptor potentials. (1999) Nature Neuroscience. 2(2):168-74. DOI: 10.1038/5729

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Lee, T.W., Girolami, M., Sejnowski, T.J. Independent component analysis using an extended infomax algorithm for mixed subgaussian and supergaussian sources. (1999) Computación neuronal. 11(2):417-41.

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Peterson, D.A., Dickinson-Anson, H.A., Leppert, J.T., Lee, K.F., Gage, F.H. Central neuronal loss and behavioral impairment in mice lacking neurotrophin receptor p75. (1999) Journal of Comparative Neurology. 404(1):1-20.

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Furuta, T., Wang, S.S., Dantzker, J.L., Dore, T.M., Bybee, W.J., Callaway, E.M., Denk, W., Tsien, R.Y. Brominated 7-hydroxycoumarin-4-ylmethyls: photolabile protecting groups with biologically useful cross-sections for two photon photolysis. (1999) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 96(4):1193-1200.

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Ernst, U., Pawelzik, K., Tsodyks, M., Sejnowski, T.J. Relation between retinotopical and orientation maps in visual cortex. (1999) Computación neuronal. 11(2):375-9.

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Takahashi, J., Palmer, T.D., Gage, F.H. Retinoic acid and neurotrophins collaborate to regulate neurogenesis in adult-derived neural stem cell cultures. (1999) Journal of Neurobiology. 38(1):65-81.

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Zhang, K., Sejnowski, T.J. Neuronal tuning: To sharpen or broaden? (1999) Computación neuronal. 11(1):75-84.

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Shihabuddin, L.S., Ray, J., Gage, F.H. Stem cell technology for basic science and clinical applications. (1999) Archives of Neurology. 56(1):29-32.

Kafri, T., van Praag, H., Ouyang, L., Gage, F.H., Verma, I.M. A packaging cell line for lentivirus vectors. (1999) Journal of Virology. 73(1):576-84.

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Mandel, R.J., Gage, F.H., Clevenger, D.G., Spratt, S.K., Snyder, R.O., Leff, S.E. Nerve growth factor expressed in the medial septum following in vivo gene delivery using a recombinant adeno-associated viral vector protects cholinergic neurons from fimbria-fornix lesion-induced deg (1999) Experimental Neurology. 155(1):59-64. DOI: 10.1006/exnr.1998.6961

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Destexhe, A., McCormick, D.A., Sejnowski, T.J. Thalamic and thalamocortical mechanisms underlying 3 Hz spike-and-wave discharges. (1999) Progress in Brain Research. 121:289-307.

Pouget, A., Deneve, S., Sejnowski, T.J. Frames of reference in hemineglect: a computational approach. (1999) Progress in Brain Research. 121:81-97.

Chiang, M.Y., Misner, D., Kempermann, G., Schikorski, T., Giguère, V., Sucov, H.M., Gage, F.H., Stevens, C.F., Evans, R.M. An essential role for retinoid receptors RARbeta and RXRgamma in long-term potentiation and depression. (1998) Neurona. 21(6):1353-61.

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Sharma, K., Sheng, H.Z., Lettieri, K., Li, H., Karavanov, A., Potter, S., Westphal, H., Pfaff, S.L. LIM homeodomain factors Lhx3 and Lhx4 assign subtype identities for motor neurons. (1998) Móvil. 95(6):817-28.

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Takahashi, M., Palmer, T.D., Takahashi, J., Gage, F.H. Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina. (1998) Molecular and Cellular Neurosciences. 12(6):340-8. DOI: 10.1006/mcne.1998.0721

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Takuma, N., Sheng, H.Z., Furuta, Y., Ward, J.M., Sharma, K., Hogan, B.L., Pfaff, S.L., Westphal, H., Kimura, S., Mahon, K.A. Formation of Rathke's pouch requires dual induction from the diencephalon. (1998) Development. 125(23):4835-40.

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Goulding, M. Specifying motor neurons and their connections. (1998) Neurona. 21(5):943-946.

Yabuta, N.H., Callaway, E.M. Cytochrome-oxidase blobs and intrinsic horizontal connections of layer 2/3 pyramidal neurons in primate V1. (1998) Visual Neuroscience. 15(6):1007-1027.

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Pyramidal neurons in superficial layers of cerebral cortex have extensive horizontal axons that provide a substrate for lateral interactions across cortical columns. These connections are believed to link functionally similar regions, as suggested by the observation that cytochrome-oxidase blobs in the monkey primary visual cortex (V1) are preferentially connected to blobs and interblobs to interblobs. To better understand the precise relationship between horizontal connections and blobs, we intracellularly labeled 20 layer 2/3 pyramidal neurons in tangential living brain slices from V1 of macaque monkeys. The locations of each cell body and the cell's synaptic boutons relative to blobs were quantitatively analyzed. We found evidence for two cell types located at characteristic distances from blob centers: (1) neurons lacking long-distance, clustered axons (somata 130-200 microm from blob centers) and (2) cells with clustered, long-distance axon collaterals (somata < 130 microm or >200 microm from blob centers). For all cells, synaptic boutons close to the cell body were located at similar distances from blob centers as the cell body. The majority of boutons from cells lacking distal axon clusters were close to their cell bodies. Cells located more than 200 microm from blob centers were in interblobs and had long-distance clustered axon collaterals selectively targeting distant interblob regions. Cells located less than 130 microm from blob centers were found within both blobs and interblobs, but many were close to traditionally defined borders. The distant synaptic boutons from these cells were generally located relatively near to blob centers, but the neurons closest to blob centers had synaptic boutons closer to blob centers than those farther away. There was not a sharp transition that would suggest specificity for blobs and interblobs as discrete, binary entities. Instead they appear to be extremes along a continuum. These observations have important implications for the function of lateral interactions within V1.

Yabuta, N.H., Callaway, E.M. Functional streams and local connections of layer 4C neurons in primary visual cortex of the macaque monkey. (1998) Diario de Neurociencia. 18(22):9489-9499.

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The primate visual system is composed of multiple, functionally specialized cortical areas. The functional diversity among areas is thought to reflect different contributions from early parallel visual pathways to the area V1 neurons providing input to "higher" cortical areas. The M pathway is believed to provide information about motion and contrast, via layer 4B of V1, to dorsal visual areas. The P pathway is believed to provide information about shape and color, via layer 2/3 of V1, to ventral visual areas, with specialized contributions from cytochrome-oxidase (CO) blob versus interblob neurons. However, the detailed anatomical relationships between the M and P pathways and the neurons in V1 that provide input to higher extrastriate cortical areas are poorly understood. To study these relationships, spiny stellate neurons in the M- and P-recipient layers of V1, 4Calpha and 4Cbeta, respectively, were intracellularly labeled, and their axonal and dendritic arbors were reconstructed. We find that neurons with dendrites in upper layer 4Calpha project axons to layer 4B and CO blobs in layer 2/3, thus relaying M input to these regions. Other neurons in lower layer 4Calpha provide M input to interblobs. These cells have either (1) dendrites restricted to lower layer 4Calpha and axons specifically targeting layer 2/3 interblobs, or (2) dendrites in lower 4Calpha and 4Cbeta and axons targeting blobs and interblobs. P-recipient layer 4Cbeta neurons have dense axonal arbors in both blobs and interblobs but not layer 4B. Quantitative analyses reveal that 4Calpha cells provide approximately five times more synapses than 4Cbeta cells to layer 4B, whereas 4Cbeta cells provide five times more synapses than 4Calpha cells to layer 2/3. These observations imply that M input is dominant in layer 4B. In layer 2/3, both blobs and interblobs receive M and P input, but the P input is dominant, and M input to interblobs derives exclusively from a subpopulation of M afferents that targets lower 4Calpha, not from afferents targeting only upper 4Calpha (cf. Blasdel and Lund, 1983). We speculate that the M and P pathways to interblobs are "X-like" linear systems, whereas blobs also receive nonlinear "Y-like" M input.

Eriksson, P.S., Perfilieva, E., Björk-Eriksson, T., Alborn, A.M., Nordborg, C., Peterson, D.A., Gage, F.H. Neurogenesis in the adult human hippocampus. (1998) Medicina Natural. 4(11):1313-7. DOI: 10.1038/3305

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Kempermann, G., Kuhn, H.G., Winkler, J., Gage, F.H. [New nerve cells for the adult brain. Adult neurogenesis and stem cell concepts in neurologic research]. (1998) Nervenarzt. 69(10):851-7.

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Gage, F.H. Stem cells of the central nervous system. (1998) Current Opinion in Neurobiology. 8(5):671-6.

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Miyoshi, H., Blömer, U., Takahashi, M., Gage, F.H., Verma, I.M. Development of a self-inactivating lentivirus vector. (1998) Journal of Virology. 72(10):8150-7.

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Varki, A., Wills, C., Perlmutter, D., Woodruff, D., Gage, F.H., Moore, J., Semendeferi, K., Bernirschke, K., Katzman, R., Doolittle, R., Bullock, T. Great Ape Phenome Project? (1998) Ciencia. 282(5387):239-40.

McKeown, M.J., Sejnowski, T.J. Independent component analysis of fMRI data: examining the assumptions. (1998) Hum Brain Mapp. 6(5-6):368-72.

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Aubert, I., Ridet, J.L., Schachner, M., Rougon, G., Gage, F.H. Expression of L1 and PSA during sprouting and regeneration in the adult hippocampal formation. (1998) Journal of Comparative Neurology. 399(1):1-19.

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Berger, F., Gage, F.H., Vijayaraghavan, S. Nicotinic receptor-induced apoptotic cell death of hippocampal progenitor cells. (1998) Diario de Neurociencia. 18(17):6871-81.

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McKeown, M.J., Humphries, C., Achermann, P., Borbély, A.A., Sejnowski, T.J. A new method for detecting state changes in the EEG: exploratory application to sleep data. (1998) Journal of Sleep Research. 7 Suppl 1:48-56.

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Bartlett, M.S., Sejnowski, T.J. Learning viewpoint-invariant face representations from visual experience in an attractor network. (1998) Network. 9(3):399-417.

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Gage, F.H., Kempermann, G., Palmer, T.D., Peterson, D.A., Ray, J. Multipotent progenitor cells in the adult dentate gyrus. (1998) Journal of Neurobiology. 36(2):249-66.

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Computational models of thalamocortical augmenting responses. (1998) Diario de Neurociencia. 18(16):6444-65.

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McKeown, M.J., Makeig, S., Brown, G.G., Jung, T.P., Kindermann, S.S., Bell, A.J., Sejnowski, T.J. Analysis of fMRI data by blind separation into independent spatial components. (1998) Hum Brain Mapp. 6(3):160-88.

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Current analytical techniques applied to functional magnetic resonance imaging (fMRI) data require a priori knowledge or specific assumptions about the time courses of processes contributing to the measured signals. Here we describe a new method for analyzing fMRI data based on the independent component analysis (ICA) algorithm of Bell and Sejnowski ([1995]: Neural Comput 7:1129-1159). We decomposed eight fMRI data sets from 4 normal subjects performing Stroop color-naming, the Brown and Peterson work/number task, and control tasks into spatially independent components. Each component consisted of voxel values at fixed three-dimensional locations (a component "map"), and a unique associated time course of activation. Given data from 144 time points collected during a 6-min trial, ICA extracted an equal number of spatially independent components. In all eight trials, ICA derived one and only one component with a time course closely matching the time course of 40-sec alternations between experimental and control tasks. The regions of maximum activity in these consistently task-related components generally overlapped active regions detected by standard correlational analysis, but included frontal regions not detected by correlation. Time courses of other ICA components were transiently task-related, quasiperiodic, or slowly varying. By utilizing higher-order statistics to enforce successively stricter criteria for spatial independence between component maps, both the ICA algorithm and a related fourth-order decomposition technique (Comon [1994]: Signal Processing 36:11-20) were superior to principal component analysis (PCA) in determining the spatial and temporal extent of task-related activation. For each subject, the time courses and active regions of the task-related ICA components were consistent across trials and were robust to the addition of simulated noise. Simulated movement artifact and simulated task-related activations added to actual fMRI data were clearly separated by the algorithm. ICA can be used to distinguish between nontask-related signal components, movements, and other artifacts, as well as consistently or transiently task-related fMRI activations, based on only weak assumptions about their spatial distributions and without a priori assumptions about their time courses. ICA appears to be a highly promising method for the analysis of fMRI data from normal and clinical populations, especially for uncovering unpredictable transient patterns of brain activity associated with performance of psychomotor tasks.

Lin, J.K., Pawelzik, K., Ernst, U., Sejnowski, T.J. Irregular synchronous activity in stochastically-coupled networks of integrate-and-fire neurons. (1998) Network. 9(3):333-44.

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Sejnowski, T.J. Neurobiology. Making smooth moves. (1998) Naturaleza. 394(6695):725-6. DOI: 10.1038/29404

Suhr, S.T., Gil, E.B., Senut, M.C., Gage, F.H. High level transactivation by a modified Bombyx ecdysone receptor in mammalian cells without exogenous retinoid X receptor. (1998) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 95(14):7999-8004.

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Winkler, J., Thal, L.J., Gage, F.H., Fisher, L.J. Cholinergic strategies for Alzheimer's disease. (1998) Journal of Molecular Medicine. 76(8):555-67.

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Alzheimer's disease is a devastating degenerative disorder of the central nervous system that results in gradual deterioration of cognitive function and severe alteration of personality. Degeneration of neurons in the nucleus basalis Meynert, the origin of the major cholinergic projections to the neocortex, occurs early in the course of the disease, and is correlated with the cognitive decline. This link between cholinergic dysfunction in the basal-cortical system and cognitive deficits has focused scientific efforts on developing tools to elucidate the neurobiological role of the cholinergic system in cognition and to develop therapeutic interventions in the disorder. An important step in understanding the mechanisms underlying cognitive dysfunction has been the development of in vivo rodent models that mimic some of the features of Alzheimer's disease. Acute excitotoxic or immunotoxic lesions of the nucleus basalis in rodents have revealed a role of the basal-cortical system in attention, learning and memory. More recent advances in developing mouse gene technology offer newer models to systematically examine the underlying neuropathological cascade leading to dysfunctions in mnemonic processing. Using in vivo rodent models, several cholinergic enhancement strategies have been tested and proven to be effective in alleviating lesion-induced cognitive deficits, including neuropharmacological approaches (acetylcholinesterase inhibitors), neurotrophic factor administration (nerve growth factor), and transplantation of cholinergic-enriched fetal grafts. Successful results have also been obtained using ex vivo gene transfer to deliver nerve growth factor or acetylcholine to compromised regions of the basal-cortical system. Gene therapy may be of particular interest for clinical applications, because this approach provides a method for topographically restricted and selective delivery of therapeutic genes and their products to afflicted areas of the brain. Advanced techniques in molecular biology (e.g., exogenous regulatable gene transfer) and newly developed tools of modern neuroscience (e.g., neural precursor cells) will be important contributions for deciphering the biological bases of neuronal degeneration and for refining therapeutic strategies for Alzheimer's disease.

McTigue, D.M., Horner, P.J., Stokes, B.T., Gage, F.H. Neurotrophin-3 and brain-derived neurotrophic factor induce oligodendrocyte proliferation and myelination of regenerating axons in the contused adult rat spinal cord. (1998) Diario de Neurociencia. 18(14):5354-65.

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Pfaff, S.L., Taylor, W.L. Xenopus TFIIIA gene transcription is dependent on cis-element positioning and chromatin structure. (1998) Biología Molecular y Celular. 18(7):3811-8.

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Kempermann, G., Brandon, E.P., Gage, F.H. Environmental stimulation of 129/SvJ mice causes increased cell proliferation and neurogenesis in the adult dentate gyrus. (1998) Biología Actual. 8(16):939-42.

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Dantzker, J.L., Callaway, E.M. The development of local, layer-specific visual cortical axons in the absence of extrinsic influences and intrinsic activity. (1998) Diario de Neurociencia. 18(11):4145-4154.

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Dickinson-Anson, H., Aubert, I., Gage, F.H., Fisher, L.J. Hippocampal grafts of acetylcholine-producing cells are sufficient to improve behavioural performance following a unilateral fimbria-fornix lesion. (1998) Neuroscience. 84(3):771-81.

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Bazhenov, M., Timofeev, I., Steriade, M., Sejnowski, T.J. Cellular and network models for intrathalamic augmenting responses during 10-Hz stimulation. (1998) Journal of Neurophysiology. 79(5):2730-48.

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Kempermann, G., Kuhn, H.G., Gage, F.H. Experience-induced neurogenesis in the senescent dentate gyrus. (1998) Diario de Neurociencia. 18(9):3206-12.

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Kempermann, G., Gage, F.H. Closer to neurogenesis in adult humans. (1998) Medicina Natural. 4(5):555-7.

Gray, M.S., Pouget, A., Zemel, R.S., Nowlan, S.J., Sejnowski, T.J. Reliable disparity estimation through selective integration. (1998) Visual Neuroscience. 15(3):511-28.

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Senut, M.C., Suhr, S.T., Gage, F.H. Gene transfer to the rodent placenta in situ. A new strategy for delivering gene products to the fetus. (1998) Revista de Investigación Clínica. 101(8):1565-71. DOI: 10.1172/JCI1959

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Moortgat, K.T., Keller, C.H., Bullock, T.H., Sejnowski, T.J. Submicrosecond pacemaker precision is behaviorally modulated: the gymnotiform electromotor pathway. (1998) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 95(8):4684-9.

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Mulle, C., Sailer, A., Pérez-Otaño, I., Dickinson-Anson, H., Castillo, P.E., Bureau, I., Maron, C., Gage, F.H., Mann, J.R., Bettler, B., Heinemann, S.F. Altered synaptic physiology and reduced susceptibility to kainate-induced seizures in GluR6-deficient mice. (1998) Naturaleza. 392(6676):601-5. DOI: 10.1038/33408

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Gage, F.H. Cell therapy. (1998) Naturaleza. 392(6679 Suppl):18-24.

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Callaway, E.M. Visual scenes and cortical neurons: what you see is what you get. (1998) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 95(7):3344-3345.

Blömer, U., Kafri, T., Randolph-Moore, L., Verma, I.M., Gage, F.H. Bcl-xL protects adult septal cholinergic neurons from axotomized cell death. (1998) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 95(5):2603-8.

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Pfaff, S., Kintner, C. Neuronal diversification: development of motor neuron subtypes. (1998) Current Opinion in Neurobiology. 8(1):27-36.

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Zhang, K., Ginzburg, I., McNaughton, B.L., Sejnowski, T.J. Interpreting neuronal population activity by reconstruction: unified framework with application to hippocampal place cells. (1998) Journal of Neurophysiology. 79(2):1017-1044.

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Physical variables such as the orientation of a line in the visual field or the location of the body in space are coded as activity levels in populations of neurons. Reconstruction or decoding is an inverse problem in which the physical variables are estimated from observed neural activity. Reconstruction is useful first in quantifying how much information about the physical variables is present in the population and, second, in providing insight into how the brain might use distributed representations in solving related computational problems such as visual object recognition and spatial navigation. Two classes of reconstruction methods, namely, probabilistic or Bayesian methods and basis function methods, are discussed. They include important existing methods as special cases, such as population vector coding, optimal linear estimation, and template matching. As a representative example for the reconstruction problem, different methods were applied to multi-electrode spike train data from hippocampal place cells in freely moving rats. The reconstruction accuracy of the trajectories of the rats was compared for the different methods. Bayesian methods were especially accurate when a continuity constraint was enforced, and the best errors were within a factor of two of the information-theoretic limit on how accurate any reconstruction can be and were comparable with the intrinsic experimental errors in position tracking. In addition, the reconstruction analysis uncovered some interesting aspects of place cell activity, such as the tendency for erratic jumps of the reconstructed trajectory when the animal stopped running. In general, the theoretical values of the minimal achievable reconstruction errors quantify how accurately a physical variable is encoded in the neuronal population in the sense of mean square error, regardless of the method used for reading out the information. One related result is that the theoretical accuracy is independent of the width of the Gaussian tuning function only in two dimensions. Finally, all the reconstruction methods considered in this paper can be implemented by a unified neural network architecture, which the brain feasibly could use to solve related problems.

Callaway, E.M. Prenatal development of layer-specific local circuits in primary visual cortex of the macaque monkey. (1998) Diario de Neurociencia. 18(4):1505-1527.

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Previous studies have demonstrated that axonal arbors specific for the four main cortical layers - 2/3, 4, 5, and 6 - develop precisely from the outset using activity-independent cues. In macaque primary visual cortex (V1), layer 2/3 is subdivided into layers named 2/3A, 3B, 4A, and 4B, and layer 4 is subdivided into 4Calpha and 4Cbeta. Individual neurons in V1 of mature macaques have axonal arbors that are highly specific for these sublayers. We have studied the prenatal development of laminar and sublaminar specificity of local circuits in macaque V1. Two-hundred thirty-eight neurons were labeled intracellularly in living brain slices prepared from V1 of five prenatal macaque monkeys aged 100 to 145 d postconception (E100-E145). Axonal and dendritic arbors of labeled neurons were reconstructed to assess their relationships to the cortical layers. We find that developing spiny neurons in layers 2-4B and layer 5 specifically target superficial and deep layers without forming "incorrect" branches in layer 4C. Similarly, layer 6 pyramidal neurons that target layer 4C do not form "incorrect" branches in layer 5. These results indicate that specific projections to the main cortical layers develop with a high degree of selectivity, as in other species. However, the development of sublayer-specific projections was not always precise from the outset. Unlike postnatal animals, axons of some prenatal layer 4Cbeta spiny neurons branch in layer 4B. At similar ages, many pyramidal neurons in the upper half of layer 6 have axonal branches in layer 4Calpha as well as 4Cbeta; these projections are specific for 4Cbeta in more mature animals. Also, there is similar "exuberance" in axonal arbors of other layer 6 cell types. Transient projections were also observed in the subplate and to the white matter for cells from all layers, except 4Cbeta. These observations indicate that at least some sublayer-specific projections emerge by elimination of exuberant axonal branches and suggest that they may use activity-dependent mechanisms to identify "correct" target layers. Such cues could be provided by laminar differences in the patterns of spontaneous prenatal activity in the retino-geniculo-cortical network.

McKeown, M.J., Jung, T.P., Makeig, S., Brown, G., Kindermann, S.S., Lee, T.W., Sejnowski, T.J. Spatially independent activity patterns in functional MRI data during the stroop color-naming task. (1998) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 95(3):803-810.

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Jurata, L.W., Pfaff, S.L., Gill, G.N. The nuclear LIM domain interactor NLI mediates homo- and heterodimerization of LIM domain transcription factors. (1998) Revista de Química Biológica. 273(6):3152-7.

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Callaway, E.M. Local circuits in primary visual cortex of the macaque monkey. (1998) Revisión Anual de Neurociencia. 21:47-74. DOI: 10.1146/annurev.neuro.21.1.47

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Zemel, R.S., Sejnowski, T.J. A model for encoding multiple object motions and self-motion in area MST of primate visual cortex. (1998) Diario de Neurociencia. 18(1):531-547.

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Berns, G.S., Sejnowski, T.J. A computational model of how the basal ganglia produce sequences. (1998) Journal of Cognitive Neuroscience. 10(1):108-121.

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Quartz, S.R., Sejnowski, T.J. The neural basis of cognitive development: a constructivist manifesto. (1997) Behavioral and Brain Sciences. 20(4):537-56; discussion 556-96.

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Shihabuddin, L.S., Ray, J., Gage, F.H. FGF-2 is sufficient to isolate progenitors found in the adult mammalian spinal cord. (1997) Exp. Neurol. 148(2):577-86. DOI: 10.1006/exnr.1997.6697

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Ridet, J.L., Malhotra, S.K., Privat, A., Gage, F.H. Reactive astrocytes: cellular and molecular cues to biological function. (1997) Trends in Neurosciences. 20(12):570-7.

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Bell, A.J., Sejnowski, T.J. The "independent components" of natural scenes are edge filters. (1997) Vision Research. 37(23):3327-3338.

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Burrill, J.D., Moran, L., Goulding, M.D., Saueressig, H. PAX2 is expressed in multiple spinal cord interneurons, including a population of EN1+ interneurons that require PAX6 for their development. (1997) Development. 124(22):4493-4503.

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Kafri, T., Blömer, U., Peterson, D.A., Gage, F.H., Verma, I.M. Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors. (1997) Nature Genetics. 17(3):314-7. DOI: 10.1038/ng1197-314

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Pouget, A., Sejnowski, T.J. A new view of hemineglect based on the response properties of parietal neurones. (1997) Philos. Trans. R. Soc. Lond., B, Biol. Sci. 352(1360):1449-1459. DOI: 10.1098/rstb.1997.0131

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Kempermann, G., Kuhn, H.G., Gage, F.H. Genetic influence on neurogenesis in the dentate gyrus of adult mice. (1997) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 94(19):10409-14.

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Miyoshi, H., Takahashi, M., Gage, F.H., Verma, I.M. Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector. (1997) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 94(19):10319-23.

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Tang, A.C., Bartels, A.M., Sejnowski, T.J. Effects of cholinergic modulation on responses of neocortical neurons to fluctuating input. (1997) Cerebral Cortex . 7(6):502-509.

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Blömer, U., Naldini, L., Kafri, T., Trono, D., Verma, I.M., Gage, F.H. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector. (1997) Journal of Virology. 71(9):6641-9.

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McWhirter, J.R., Goulding, M., Weiner, J.A., Chun, J., Murre, C. A novel fibroblast growth factor gene expressed in the developing nervous system is a downstream target of the chimeric homeodomain oncoprotein E2A-Pbx1. (1997) Development. 124(17):3221-3232.

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Makeig, S., Jung, T.P., Bell, A.J., Ghahremani, D., Sejnowski, T.J. Blind separation of auditory event-related brain responses into independent components. (1997) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 94(20):10979-10984.

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Ritz, R., Sejnowski, T.J. Synchronous oscillatory activity in sensory systems: new vistas on mechanisms. (1997) Current Opinion in Neurobiology. 7(4):536-546.

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Kuhn, H.G., Winkler, J., Kempermann, G., Thal, L.J., Gage, F.H. Epidermal growth factor and fibroblast growth factor-2 have different effects on neural progenitors in the adult rat brain. (1997) Diario de Neurociencia. 17(15):5820-9.

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Peterson, D.A., Leppert, J.T., Lee, K.F., Gage, F.H. Basal forebrain neuronal loss in mice lacking neurotrophin receptor p75. (1997) Ciencia. 277(5327):837-9.

Spitzer, N.C., Sejnowski, T.J. Biological information processing: bits of progress. (1997) Ciencia. 277(5329):1060-1061.

Grill, R., Murai, K., Blesch, A., Gage, F.H., Tuszynski, M.H. Cellular delivery of neurotrophin-3 promotes corticospinal axonal growth and partial functional recovery after spinal cord injury. (1997) Diario de Neurociencia. 17(14):5560-72.

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Schinstine, M., Ray, J., Gage, F.H. Potential effect of cytokines on transgene expression in primary fibroblasts implanted into the rat brain. (1997) Brain Res. Mol. Brain Res. 47(1-2):195-201.

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Ray, J., Baird, A., Gage, F.H. A 10-amino acid sequence of fibroblast growth factor 2 is sufficient for its mitogenic activity on neural progenitor cells. (1997) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 94(13):7047-52.

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Palmer, T.D., Takahashi, J., Gage, F.H. The adult rat hippocampus contains primordial neural stem cells. (1997) Molecular and Cellular Neurosciences. 8(6):389-404. DOI: 10.1006/mcne.1996.0595

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Sah, D.W., Ray, J., Gage, F.H. Bipotent progenitor cell lines from the human CNS. (1997) Nature Biotechnology. 15(6):574-80. DOI: 10.1038/nbt0697-574

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Tsodyks, M.V., Skaggs, W.E., Sejnowski, T.J., McNaughton, B.L. Paradoxical effects of external modulation of inhibitory interneurons. (1997) Diario de Neurociencia. 17(11):4382-4388.

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Bang, A.G., Papalopulu, N., Kintner, C., Goulding, M.D. Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm. (1997) Development. 124(10):2075-85.

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Kempermann, G., Kuhn, H.G., Gage, F.H. More hippocampal neurons in adult mice living in an enriched environment. (1997) Naturaleza. 386(6624):493-5. DOI: 10.1038/386493a0

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Murthy, V.N., Sejnowski, T.J., Stevens, C.F. Heterogeneous release properties of visualized individual hippocampal synapses. (1997) Neurona. 18(4):599-612.

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Fagan, A.M., Suhr, S.T., Lucidi-Phillipi, C.A., Peterson, D.A., Holtzman, D.M., Gage, F.H. Endogenous FGF-2 is important for cholinergic sprouting in the denervated hippocampus. (1997) Diario de Neurociencia. 17(7):2499-511.

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Maroto, M., Reshef, R., Münsterberg, A.E., Koester, S., Goulding, M., Lassar, A.B. Ectopic Pax-3 activates MyoD and Myf-5 expression in embryonic mesoderm and neural tissue. (1997) Móvil. 89(1):139-148.

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Raymon, H.K., Thode, S., Gage, F.H. Application of ex vivo gene therapy in the treatment of Parkinson's disease. (1997) Exp. Neurol. 144(1):82-91. DOI: 10.1006/exnr.1996.6392

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Wiser, A.K., Callaway, E.M. Ocular dominance columns and local projections of layer 6 pyramidal neurons in macaque primary visual cortex. (1997) Developmental Biology. 14(2):241-251.

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Pouget, A., Sejnowski, T.J. Spatial transformations in the parietal cortex using basis functions. (1997) Journal of Cognitive Neuroscience. 9(2):222-237. DOI: 10.1162/jocn.1997.9.2.222

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Contreras, D., Destexhe, A., Sejnowski, T.J., Steriade, M. Spatiotemporal patterns of spindle oscillations in cortex and thalamus. (1997) Diario de Neurociencia. 17(3):1179-1196.

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Spindle oscillations (7-14 Hz) appear in the thalamus and cortex during early stages of sleep. They are generated by the combination of intrinsic properties and connectivity patterns of thalamic neurons and distributed to cortical territories by thalamocortical axons. The corticothalamic feedback is a major factor in producing coherent spatiotemporal maps of spindle oscillations in widespread thalamic territories. Here we have investigated the spatiotemporal patterns of spontaneously occurring and evoked spindles by means of multisite field potential and unit recordings in intact cortex and decorticated animals. We show that (1) spontaneous spindle oscillations are synchronized over large cortical areas during natural sleep and barbiturate anesthesia; (2) under barbiturate anesthesia, the cortical coherence is not disrupted by transection of intracortical synaptic linkages; (3) in intact cortex animals, spontaneously occurring barbiturate spindle sequences occur nearly simultaneously over widespread thalamic territories; (4) in the absence of cortex, the spontaneous spindle oscillations throughout the thalamus are less organized, but the local coherence (within 2-4 mm) is still maintained; and (5) spindling propagation is observed in intact cortex animals only when elicited by low intensity cortical stimulation, applied shortly before the initiation of a spontaneous spindle sequence; propagation velocities are between 1 and 3 mm/sec, measured in the anteroposterior axis of the thalamus; increasing the intensity of cortical stimulation triggers spindle oscillations, which start simultaneously in all leads. We propose that, in vivo, the coherence of spontaneous spindle oscillations in corticothalamic networks is attributable to the combined action of continuous background corticothalamic input initiating spindle sequences in several thalamic sites at the same time and divergent corticothalamic and intrathalamic connectivity.

Sejnowski, T.J. The year of the dendrite. (1997) Ciencia. 275(5297):178-179.

Jung, T.P., Makeig, S., Stensmo, M., Sejnowski, T.J. Estimating alertness from the EEG power spectrum. (1997) IEEE Trans Biomed Eng. 44(1):60-69. DOI: 10.1109/10.553713

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Ahlgren, U., Pfaff, S.L., Jessell, T.M., Edlund, T., Edlund, H. Independent requirement for ISL1 in formation of pancreatic mesenchyme and islet cells. (1997) Naturaleza. 385(6613):257-60. DOI: 10.1038/385257a0

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Winkler, J., Ramirez, G.A., Kuhn, H.G., Peterson, D.A., Day-Lollini, P.A., Stewart, G.R., Tuszynski, M.H., Gage, F.H., Thal, L.J. Reversible Schwann cell hyperplasia and sprouting of sensory and sympathetic neurites after intraventricular administration of nerve growth factor. (1997) Annals of Neurology. 41(1):82-93. DOI: 10.1002/ana.410410114

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Sah, D.W., Ray, J., Gage, F.H. Regulation of voltage- and ligand-gated currents in rat hippocampal progenitor cells in vitro. (1997) Journal of Neurobiology. 32(1):95-110.

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Callaway, E.M., Lieber, J.L. Development of axonal arbors of layer 6 pyramidal neurons in ferret primary visual cortex. (1996) Journal of Comparative Neurology. 376(2):295-305. DOI: 10.1002/(SICI)1096-9861(19961209)376:2<295::AID

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Senut, M.C., Suhr, S.T., Gage, F.H. Intracerebral xenografts: Sertoli cells to the rescue? (1996) Nature Biotechnology. 14(13):1650-1. DOI: 10.1038/nbt1296-1650

Contreras, D., Destexhe, A., Sejnowski, T.J., Steriade, M. Control of spatiotemporal coherence of a thalamic oscillation by corticothalamic feedback. (1996) Ciencia. 274(5288):771-774.

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Suhonen, J.O., Peterson, D.A., Ray, J., Gage, F.H. Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo. (1996) Naturaleza. 383(6601):624-7. DOI: 10.1038/383624a0

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Naldini, L., Blömer, U., Gage, F.H., Trono, D., Verma, I.M. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. (1996) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 93(21):11382-8.

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Lipkin, S.M., Grider, T.L., Heyman, R.A., Glass, C.K., Gage, F.H. Constitutive retinoid receptors expressed from adenovirus vectors that specifically activate chromosomal target genes required for differentiation of promyelocytic leukemia and teratocarcinoma cells. (1996) Journal of Virology. 70(10):7182-9.

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Sufficient knowledge of transcription factor structure and function has accumulated to allow attempts at the rational design of novel transcription factors for the study of gene regulation and potential application in gene therapy. In the present studies, we have systematically evaluated the function of chimeric retinoid receptors generated by fusion with the transactivation domain of VP16 and expression in adenovirus vectors. By varying the location of fusion of the VP16 transactivation domain with the retinoic acid receptor (RAR) or retinoid X receptor (RXR), marked differences in the specificity of gene activation were obtained. Although several chimeric proteins activated both RAR and RXR target genes, fusion of the NT16 transactivation domain to the N terminus of RAR permitted specific activation of reporter genes containing retinoic acid response elements. In contrast, fusion of the VP16 transactivation domain to the C terminus of RXR permitted specific activation of reporter genes containing RXR response elements. When tested for their ability to activate chromosomal targets, the chimera consisting of VP16 linked to the N terminus of PAR was much more active in promoting the differentiation of HL-60 cells and NTera-2 cells than the chimera consisting of VP16 linked to the C terminus of RXR. These observations support the existence of two distinct retinoid signalling pathways predicted on the basis of biochemical and pharmacologic studies and provide direct evidence that the programs of differentiation elicited by retinoic acid in these cells are mediated by a specific subset of binding sites for RAR-RXR heterodimers. VP16-RAR and VP16-RXR fusion proteins should be of further use in dissecting the relative contributions of RARs and RXRs to specific programs of gene expression. Constitutive retinoid receptors may also be considered for use as novel tumor suppressor genes for genetically based treatment of retinoid-responsive cancers.

Choi, D.W., Gage, F.H. Disease, transplantation and regeneration. (1996) Current Opinion in Neurobiology. 6(5):635-7.

Varela-Echavarría, A., Pfaff, S.L., Guthrie, S. Differential expression of LIM homeobox genes among motor neuron subpopulations in the developing chick brain stem. (1996) Molecular and Cellular Neurosciences. 8(4):242-57. DOI: 10.1006/mcne.1996.0061

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Callaway, E.M., Wiser, A.K. Contributions of individual layer 2-5 spiny neurons to local circuits in macaque primary visual cortex. (1996) Visual Neuroscience. 13(5):907-922.

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Minger, S.L., Fisher, L.J., Ray, J., Gage, F.H. Long-term survival of transplanted basal forebrain cells following in vitro propagation with fibroblast growth factor-2. (1996) Exp. Neurol. 141(1):12-24. DOI: 10.1006/exnr.1996.0134

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The intracerebral transplantation of freshly dissected fetal tissue containing cholinergic neurons of the developing basal forebrain has been reported to reverse lesion-induced or age-related cognitive deficits in animal models of cholinergic neuronal degeneration. Grafts of cultured fetal neurons, however, have generally shown poor cellular survival and limited therapeutic benefit. We tested the hypothesis that recent advances in the identification of growth factors that promote the survival and propagation of fetal precursor cells in vitro would improve the long-term survival of cultured neurons following intracerebral implantation. Dissociated cells from gestational Day 14 rodent basal forebrain were grown in chemically defined media supplemented with 20 ng/ml basic fibroblast growth factor. Two weeks postplating, numerous cells were present in the cultures and showed immunoreactive labeling for a variety of markers, including glutamic acid decarboxylase, neuron-specific enolase, neurofilament proteins, glial fibrillary acidic protein and, occasionally, choline acetyltransferase. To determine if cultured basal forebrain cells would survive intracerebral implantation, the cells were implanted homotypically into the nucleus basalis magnocellularis. To enhance the potential for graft survival in vivo, cells were also implanted into the nucleus basalis magnocellularis following an ibotenic acid lesion and into the denervated frontal cortex. Animals sacrificed between 2 weeks and 7 months following transplantation showed good and comparable graft survival in all sites. Immunocytochemical analysis revealed that representative populations of cells observed in vitro survived for prolonged periods in vivo, even in sites distal from their normal cellular targets. Thus, neuronal populations expanded in vitro can successfully survive and maintain cellular phenotypes post-transplantation. These results suggest a potential for isolating and growing specific neuronal populations in vitro for intracerebral transplantation.

Destexhe, A., Bal, T., McCormick, D.A., Sejnowski, T.J. Ionic mechanisms underlying synchronized oscillations and propagating waves in a model of ferret thalamic slices. (1996) Journal of Neurophysiology. 76(3):2049-2070.

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1. A network model of thalamocortical (TC) and thalamic reticular (RE) neurons was developed based on electrophysiological measurements in ferret thalamic slices. Single-compartment TC and RE cells included voltage- and calcium-sensitive currents described by Hodgkin-Huxley type of kinetics. Synaptic currents were modeled by kinetic models of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), gamma-aminobutyric acid-A (GABAA) and GABAB receptors. 2. The model reproduced successfully the characteristics of spindle and slow bicuculline-induced oscillations observed in vitro. The characteristics of these two types of oscillations depended on both the intrinsic properties of TC and RE cells and their pattern of interconnectivity. 3. The oscillations were organized by the reciprocal recruitment between TC and RE cells, due to their manual connectivity and bursting properties. TC cells elicited AMPA-mediated excitatory postsynaptic potentials (EPSPs) in RE cells, whereas RE cells elicited a mixture of GABAA and GABAB inhibitory postsynaptic potentials (IPSPs) in TC cells. Because of the presence of a T current, sufficiently strong EPSPs could elicit a burst in RE cells, and TC cells could generate a rebound burst following GABAergic IPSPs. Under these conditions, interaction between the TC and RE cells produced sustained oscillations. 4. In the absence of spontaneous oscillation in any cell, the TC-RE network remained quiescent. Spindle oscillations with a frequency of 9-11 Hz could be initiated by stimulation of either TC or RE neurons. A few spontaneously oscillating TC neurons recruited the entire network model into a "waxing-and waning" oscillation. These "initiator" cells could be an extremely small proportion of TC cells. 5. In intracellular recordings, TC cells display a reduced ability for burst firing after a sequence of bursts. The "waning" phase of spindles was reproduced in the network model by assuming an activity-dependent upregulation of Ih operating via a calcium-binding protein in TC cells, as shown previously in a two-cell model. 6. Following the global suppression of GABAA inhibition, the disinhibited RE cells produced prolonged burst discharges that elicited strong GABAB-mediated currents in TC cells. The enhancement of slow IPSPs in TC cells was also due to cooperativity in the activation of GABAB-mediated current. These slow IPSPs recruited TC and RE cells into slower waxing-and-waning oscillations (3-4 HZ) that were even more highly synchronized. 7. Local axonal arborization of the TC to RE and RE to TC projections allowed oscillations to propagate through the network. An oscillation starting at a single focus induced a propagating wavefront as more cells were recruited progressively. The waning of the oscillation also propagated due to upregulation of Ih in TC cells, leading to waves of spindle activity as observed in experiments. 8. The spatiotemporal properties of propagating waves in the model were highly dependent on the intrinsic properties of TC cells. The spatial pattern of spiking activity was markedly different for spindles compared with bicuculline-induced oscillations and depended on the rebound burst behavior of TC cells. The upregulation of Ih produced a refractory period so that colliding spindle waves merged into a single oscillation and extinguished. Finally, reducing the Ih conductance led to sustained oscillations. 9. Two key properties of cells in the thalamic network may account for the initiation, propagation, and termination of spindle oscillations, the activity-dependent upregulation of Ih in TC cells, and the localized axonal projections between TC and RE cells. In addition, the model predicts that a nonlinear stimulus dependency of GABAB responses accounts for the genesis of prolonged synchronized discharges following block of GABAA receptors.

Nakajima, T., Fukamizu, A., Takahashi, J., Gage, F.H., Fisher, T., Blenis, J., Montminy, M.R. The signal-dependent coactivator CBP is a nuclear target for pp90RSK. (1996) Móvil. 86(3):465-74.

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Sejnowski, T.J. Synapses get smarter. (1996) Naturaleza. 382(6594):759-760. DOI: 10.1038/382759a0

Prank, K., Kloppstech, M., Nowlan, S.J., Sejnowski, T.J., Brabant, G. Random Secretion of Growth Hormone in Humans. (1996) Phys. Rev. Lett. 77(9):1909-1911. DOI: 10.1103/PhysRevLett.77.1909

Mainen, Z.F., Sejnowski, T.J. Influence of dendritic structure on firing pattern in model neocortical neurons. (1996) Naturaleza. 382(6589):363-366. DOI: 10.1038/382363a0

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Chalmers, G.R., Peterson, D.A., Gage, F.H. Sprouting adult CNS cholinergic axons express NILE and associate with astrocytic surfaces expressing neural cell adhesion molecule. (1996) Journal of Comparative Neurology. 371(2):287-99. DOI: 10.1002/(SICI)1096-9861(19960722)371:2<287::AID

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Nakahara, Y., Gage, F.H., Tuszynski, M.H. Grafts of fibroblasts genetically modified to secrete NGF, BDNF, NT-3, or basic FGF elicit differential responses in the adult spinal cord. (1996) Cell Transplantation. 5(2):191-204.

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Prank, K., Kloppstech, M., Nowlan, S.J., Sejnowski, T.J., Brabant, G. Self-organized segmentation of time series: separating growth hormone secretion in acromegaly from normal controls. (1996) Biophysical Journal. 70(6):2540-2547. DOI: 10.1016/S0006-3495(96)79825-9

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Tsodyks, M.V., Skaggs, W.E., Sejnowski, T.J., McNaughton, B.L. Population dynamics and theta rhythm phase precession of hippocampal place cell firing: a spiking neuron model. (1996) Hipocampo. 6(3):271-280. DOI: 10.1002/(SICI)1098-1063(1996)6:3<271::AID-HIPO5

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Bell, A.J., Sejnowski, T.J. Learning the higher-order structure of a natural sound. (1996) Network. 7(2):261-267. DOI: 10.1088/0954-898X/7/2/005

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Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F.H., Verma, I.M., Trono, D. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. (1996) Ciencia. 272(5259):263-7.

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Sawatari, A., Callaway, E.M. Convergence of magno- and parvocellular pathways in layer 4B of macaque primary visual cortex. (1996) Naturaleza. 380(6573):442-446. DOI: 10.1038/380442a0

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Wiser, A.K., Callaway, E.M. Contributions of individual layer 6 pyramidal neurons to local circuitry in macaque primary visual cortex. (1996) Diario de Neurociencia. 16(8):2724-2739.

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Tuszynski, M.H., Roberts, J., Senut, M.C., U, H.S., Gage, F.H. Gene therapy in the adult primate brain: intraparenchymal grafts of cells genetically modified to produce nerve growth factor prevent cholinergic neuronal degeneration. (1996) Gene Therapy. 3(4):305-14.

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Kuhn, H.G., Dickinson-Anson, H., Gage, F.H. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. (1996) Diario de Neurociencia. 16(6):2027-33.

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Lucidi-Phillipi, C.A., Clary, D.O., Reichardt, L.F., Gage, F.H. TrkA activation is sufficient to rescue axotomized cholinergic neurons. (1996) Neurona. 16(3):653-63.

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Daston, G., Lamar, E., Olivier, M., Goulding, M. Pax-3 is necessary for migration but not differentiation of limb muscle precursors in the mouse. (1996) Development. 122(3):1017-1027.

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Tuszynski, M.H., Gage, F.H. Somatic gene therapy for nervous system disease. (1996) Ciba Foundation Symposium. 196:85-94; discussion 94-7.

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Montague, P.R., Dayan, P., Sejnowski, T.J. A framework for mesencephalic dopamine systems based on predictive Hebbian learning. (1996) Diario de Neurociencia. 16(5):1936-1947.

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Hoshimaru, M., Ray, J., Sah, D.W., Gage, F.H. Differentiation of the immortalized adult neuronal progenitor cell line HC2S2 into neurons by regulatable suppression of the v-myc oncogene. (1996) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 93(4):1518-23.

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Bang, A.G., Goulding, M.D. Regulation of vertebrate neural cell fate by transcription factors. (1996) Current Opinion in Neurobiology. 6(1):25-32.

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Peterson, D.A., Lucidi-Phillipi, C.A., Murphy, D.P., Ray, J., Gage, F.H. Fibroblast growth factor-2 protects entorhinal layer II glutamatergic neurons from axotomy-induced death. (1996) Diario de Neurociencia. 16(3):886-98.

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Lytton, W.W., Destexhe, A., Sejnowski, T.J. Control of slow oscillations in the thalamocortical neuron: a computer model. (1996) Neuroscience. 70(3):673-684.

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We investigated computer models of a single thalamocortical neuron to assess the interaction of intrinsic voltage-sensitive channels and cortical synaptic input in producing the range of oscillation frequencies observed in these cells in vivo. A morphologically detailed model with Hodgkin-Huxley-like ion channels demonstrated that intrinsic properties would be sufficient to readily produce 3 to 6 Hz oscillations. Hyperpolarization of the model cell reduced its oscillation frequency monotonically whether through current injection or modulation of a potassium conductance, simulating the response to a neuromodulatory input. We performed detailed analysis of highly reduced models to determine the mechanism of this frequency control. The interburst interval was controlled by two different mechanisms depending on whether or not the pacemaker current, IH, was present. In the absence of IH, depolarization during the interburst interval occurred at the same rate with different current injections. The voltage difference from the nadir to threshold for the low-threshold calcium current, IT, determined the interburst interval. In contrast, with IH present, the rate of depolarization depended on injected current. With the full model, simulated repetitive cortical synaptic input entrained oscillations up to approximately double the natural frequency. Cortical input readily produced phase resetting as well. Our findings suggest that neither ascending brainstem control altering underlying hyperpolarization, nor descending drive by repetitive cortical inputs, would alone be sufficient to produce the range of oscillation frequencies seen in thalamocortical neurons. Instead, intrinsic neuronal mechanisms would dominate for generating the delta range (0.5-4 Hz) oscillations seen during slow wave sleep, whereas synaptic interactions with cortex and the thalamic reticular nucleus would be required for faster oscillations in the frequency range of spindling (7-14 Hz).

Blömer, U., Naldini, L., Verma, I.M., Trono, D., Gage, F.H. Applications of gene therapy to the CNS. (1996) Genética Molecular Humana. 5 Spec No:1397-404.

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Kilpatrick, T.J., Brown, A., Lai, C., Gassmann, M., Goulding, M., Lemke, G. Expression of the Tyro4/Mek4/Cek4 gene specifically marks a subset of embryonic motor neurons and their muscle targets. (1996) Molecular and Cellular Neurosciences. 7(1):62-74. DOI: 10.1006/mcne.1996.0005

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Destexhe, A., Contreras, D., Steriade, M., Sejnowski, T.J., Huguenard, J.R. In vivo, in vitro, and computational analysis of dendritic calcium currents in thalamic reticular neurons. (1996) Diario de Neurociencia. 16(1):169-185.

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Pfaff, S.L., Mendelsohn, M., Stewart, C.L., Edlund, T., Jessell, T.M. Requirement for LIM homeobox gene Isl1 in motor neuron generation reveals a motor neuron-dependent step in interneuron differentiation. (1996) Móvil. 84(2):309-20.

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Tuszynski, M.H., Gabriel, K., Gage, F.H., Suhr, S., Meyer, S., Rosetti, A. Nerve growth factor delivery by gene transfer induces differential outgrowth of sensory, motor, and noradrenergic neurites after adult spinal cord injury. (1996) Experimental Neurology. 137(1):157-73. DOI: 10.1006/exnr.1996.0016

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Eagle, K.S., Chalmers, G.R., Clary, D.O., Gage, F.H. Axonal regeneration and limited functional recovery following hippocampal deafferentation. (1995) Journal of Comparative Neurology. 363(3):377-88. DOI: 10.1002/cne.903630304

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Gage, F.H., Coates, P.W., Palmer, T.D., Kuhn, H.G., Fisher, L.J., Suhonen, J.O., Peterson, D.A., Suhr, S.T., Ray, J. Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. (1995) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 92(25):11879-83.

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Mainen, Z.F., Joerges, J., Huguenard, J.R., Sejnowski, T.J. A model of spike initiation in neocortical pyramidal neurons. (1995) Neurona. 15(6):1427-1439.

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Ray, J., Raymon, H.K., Gage, F.H. Generation and culturing of precursor cells and neuroblasts from embryonic and adult central nervous system. (1995) Methods in Enzymology. 254:20-37.

Gray, M.S., Lawrence, D.T., Golomb, B.A., Sejnowski, T.J. A perceptron reveals the face of sex. (1995) Computación neuronal. 7(6):1160-1164.

Bell, A.J., Sejnowski, T.J. An information-maximization approach to blind separation and blind deconvolution. (1995) Computación neuronal. 7(6):1129-1159.

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Destexhe, A., Sejnowski, T.J. G protein activation kinetics and spillover of gamma-aminobutyric acid may account for differences between inhibitory responses in the hippocampus and thalamus. (1995) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 92(21):9515-9519.

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Palmer, T.D., Ray, J., Gage, F.H. FGF-2-responsive neuronal progenitors reside in proliferative and quiescent regions of the adult rodent brain. (1995) Molecular and Cellular Neurosciences. 6(5):474-86. DOI: 10.1006/mcne.1995.1035

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Aubert, I., Ridet, J.L., Gage, F.H. Regeneration in the adult mammalian CNS: guided by development. (1995) Current Opinion in Neurobiology. 5(5):625-35.

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Montague, P.R., Dayan, P., Person, C., Sejnowski, T.J. Bee foraging in uncertain environments using predictive hebbian learning. (1995) Naturaleza. 377(6551):725-728. DOI: 10.1038/377725a0

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Senut, M.C., Tuszynski, M.H., Raymon, H.K., Suhr, S.T., Liou, N.H., Jones, K.R., Reichardt, L.F., Gage, F.H. Regional differences in responsiveness of adult CNS axons to grafts of cells expressing human neurotrophin 3. (1995) Experimental Neurology. 135(1):36-55. DOI: 10.1006/exnr.1995.1064

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Chen, K.S., Masliah, E., Mallory, M., Gage, F.H. Synaptic loss in cognitively impaired aged rats is ameliorated by chronic human nerve growth factor infusion. (1995) Neuroscience. 68(1):19-27.

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Sejnowski, T.J. Neural networks. Sleep and memory. (1995) Biología Actual. 5(8):832-834.

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Fisher, L.J., Gage, F.H. Novel therapeutic directions for Parkinson's disease. (1995) Mol Med Today. 1(4):181-7.

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Sejnowski, T.J. Pattern recognition. Time for a new neural code? (1995) Naturaleza. 376(6535):21-22. DOI: 10.1038/376021a0

Mainen, Z.F., Sejnowski, T.J. Reliability of spike timing in neocortical neurons. (1995) Ciencia. 268(5216):1503-1506.

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Prank, K., Nowlan, S.J., Harms, H.M., Kloppstech, M., Brabant, G., Hesch, R.D., Sejnowski, T.J. Time series prediction of plasma hormone concentration. Evidence for differences in predictability of parathyroid hormone secretion between osteoporotic patients and normal controls. (1995) Revista de Investigación Clínica. 95(6):2910-2919. DOI: 10.1172/JCI117998

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Winkler, J., Suhr, S.T., Gage, F.H., Thal, L.J., Fisher, L.J. Essential role of neocortical acetylcholine in spatial memory. (1995) Naturaleza. 375(6531):484-7. DOI: 10.1038/375484a0

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Tuszynski, M.H., Gage, F.H. Maintaining the neuronal phenotype after injury in the adult CNS. Neurotrophic factors, axonal growth substrates, and gene therapy. (1995) Molecular Neurobiology. 10(2-3):151-67. DOI: 10.1007/BF02740673

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Gage, F.H., Ray, J., Fisher, L.J. Isolation, characterization, and use of stem cells from the CNS. (1995) Revisión Anual de Neurociencia. 18:159-92. DOI: 10.1146/annurev.ne.18.030195.001111

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The nervous system of adult mammals, unlike the rest of the organs in the body, has been considered unique in its apparent inability to replace neurons following injury. However, in certain regions of the brain, neurogenesis occurs postnatally and continues through adulthood. The nature, fate, and longevity of cells undergoing proliferation within the CNS are unknown. These cells are increasingly becoming the focus of intense scrutiny; this is a recent development that has led to considerable controversy over the appropriate terminology to describe neural cells as they pass through different stages of proliferation, migration, and differentiation. Continuing studies detailing the properties of mitotic populations in the adult CNS will provide a better understanding of the nature of these cells during their development and should lead to a more consistent nomenclature. Studies of neural precursors isolated from the embryonic brain have indicated that many subgroups of cells undergo mitosis and subsequent differentiation into neurons and glia in vitro. A number of substances, such as growth factors and substrate molecules, are essential for these processes and also for lineage restriction and fate determination of these cells. Recent studies have shown that cells with proliferative capabilities can also be isolated from the adult brain. The nature of these cells is unknown, but there is evidence that both multipotent cells (stem cells) and lineage-restricted cells (neuroblasts or glioblasts) are resident within the mature CNS and that they can be maintained and induced to divide and differentiate in response to many of the same factors that influence their embryonic counterparts. Presently, it is unclear how many potentially quiescent precursor cells exist in the adult brain or what combination of growth factors and substrate molecules is involved in the proliferation and differentiation of these cells. Some of these questions are currently being addressed by using immortalized neural precursors or growth factor-expanded populations of primary precursors to model precursor responsiveness to environmental manipulations. Because in vitro culture conditions are unlikely to provide all of the factors necessary for inducing the proliferation and differentiation of neural precursors, recent studies have explored the properties of well-characterized precursor populations after implantation back into specific regions of the developing or adult CNS. These studies have highlighted the importance of the microenvironment in precursor differentiation and further suggested that precursor plasticity is a characteristic that is probably common to neural precursors throughout the CNS.(ABSTRACT TRUNCATED AT 400 WORDS)

du Lac, S., Raymond, J.L., Sejnowski, T.J., Lisberger, S.G. Learning and memory in the vestibulo-ocular reflex. (1995) Revisión Anual de Neurociencia. 18:409-441. DOI: 10.1146/annurev.ne.18.030195.002205

Jester, J.M., Campbell, L.W., Sejnowski, T.J. Associative EPSP--spike potentiation induced by pairing orthodromic and antidromic stimulation in rat hippocampal slices. (1995) Journal of Physiology. 484 ( Pt 3:689-705.

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Tuszynski, M.H., Gage, F.H. Bridging grafts and transient nerve growth factor infusions promote long-term central nervous system neuronal rescue and partial functional recovery. (1995) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 92(10):4621-5.

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Chalmers, G.R., Fisher, L.J., Niijima, K., Patterson, P.H., Gage, F.H. Adrenal chromaffin cells transdifferentiate in response to basic fibroblast growth factor and show directed outgrowth to a nerve growth factor source in vivo. (1995) Exp. Neurol. 133(1):32-42. DOI: 10.1006/exnr.1995.1005

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Chromaffin cells exposed to basic fibroblast growth factor (bFGF) in vitro express characteristics of sympathetic neurons, extend neurites, and become dependent on nerve growth factor (NGF) for survival. We explored whether the growth factor responsiveness of chromaffin cells could be exploited to enhance the transdifferentiation, neurite outgrowth and functional efficacy of chromaffin cells implanted into rats with unilateral 6-hydroxydopamine lesions. Cografts of neonatal chromaffin cells and fibroblasts genetically modified to produce bFGF were placed into the dopamine-depleted striatum of adult rats. Either control-transfected or NGF-producing fibroblasts were then transplanted 1 mm distal to the cograft. Chromaffin cells transdifferentiated under the influence of bFGF, as indicated by the growth of neurites and the expression of neuron-specific proteins. Distal grafts of NGF-producing fibroblasts successfully induced chromaffin neurites to traverse through the host parenchyma to the NGF source. In the absence of NGF fibroblast grafts, neither neurite extension nor good, long-term survival of the chromaffin-derived neurons was observed. Assessments of apomorphine-induced rotational behavior 2- and 4-weeks postgrafting revealed no behavior improvements in any of the groups. These results indicate that localized sources of growth factors are effective in inducing the transdifferentiation of grafted chromaffin cells as well as the extension of chromaffin-derived neurites into the host parenchyma. Such chromaffin cell-derived neurons are, however, functionally ineffective in this rat model of Parkinson's disease. Whether the lack of behavioral improvement reflected the tropic growth of neurites to an inappropriate striatal region or the noradrenergic nature of the chromaffin cell-derived neurons remains to be clarified. Nonetheless, these results caution that promoting transdifferentiation and neurite extension from engrafted chromaffin cells may not be sufficient to achieve desired functional effects of such grafts.

Lucidi-Phillipi, C.A., Gage, F.H., Shults, C.W., Jones, K.R., Reichardt, L.F., Kang, U.J. Brain-derived neurotrophic factor-transduced fibroblasts: production of BDNF and effects of grafting to the adult rat brain. (1995) Journal of Comparative Neurology. 354(3):361-76. DOI: 10.1002/cne.903540306

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Chen, K.S., Gage, F.H. Somatic gene transfer of NGF to the aged brain: behavioral and morphological amelioration. (1995) Diario de Neurociencia. 15(4):2819-25.

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Fisher, L.J., Gage, F.H. Radical directions in Parkinson's disease. (1995) Medicina Natural. 1(3):201-3.

Nowlan, S.J., Sejnowski, T.J. A selection model for motion processing in area MT of primates. (1995) Diario de Neurociencia. 15(2):1195-1214.

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Niijima, K., Chalmers, G.R., Peterson, D.A., Fisher, L.J., Patterson, P.H., Gage, F.H. Enhanced survival and neuronal differentiation of adrenal chromaffin cells cografted into the striatum with NGF-producing fibroblasts. (1995) Diario de Neurociencia. 15(2):1180-94.

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Ray, J., Hogg, J., Beutler, A.S., Takayama, H., Baird, A., Gage, F.H. Expression of biologically active basic fibroblast growth factor by genetically modified rat primary skin fibroblasts. (1995) Journal of Neurochemistry. 64(2):503-13.

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Beutler, A.S., Banck, M.S., Bach, F.W., Gage, F.H., Porreca, F., Bilsky, E.J., Yaksh, T.L. Retrovirus-mediated expression of an artificial beta-endorphin precursor in primary fibroblasts. (1995) J. Neurochem. 64(2):475-81.

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Takayama, H., Ray, J., Raymon, H.K., Baird, A., Hogg, J., Fisher, L.J., Gage, F.H. Basic fibroblast growth factor increases dopaminergic graft survival and function in a rat model of Parkinson's disease. (1995) Medicina Natural. 1(1):53-8.

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Tsuchida, T., Ensini, M., Morton, S.B., Baldassare, M., Edlund, T., Jessell, T.M., Pfaff, S.L. Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. (1994) Móvil. 79(6):957-70.

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Gonzalez, A.M., Carman, L.S., Ong, M., Ray, J., Gage, F.H., Shults, C.W., Baird, A. Storage, metabolism, and processing of 125I-fibroblast growth factor-2 after intracerebral injection. (1994) Brain Research. 665(2):285-92.

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Callaway, E.M. Caged neurotransmitters. Shedding light on neural circuits. (1994) Biología Actual. 4(11):1010-1012.

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Peterson, D.A., Lucidi-Phillipi, C.A., Eagle, K.L., Gage, F.H. Perforant path damage results in progressive neuronal death and somal atrophy in layer II of entorhinal cortex and functional impairment with increasing postdamage age. (1994) Diario de Neurociencia. 14(11 Pt 2):6872-85.

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Destexhe, A., Contreras, D., Sejnowski, T.J., Steriade, M. Modeling the control of reticular thalamic oscillations by neuromodulators. (1994) Neuroreport. 5(17):2217-2220.

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Choi, D., Gage, F.H. Disease, transplantation and regeneration. (1994) Current Opinion in Neurobiology. 4(5):693-5.

Tuszynski, M.H., Gage, F.H. Neurotrophic factors and diseases of the nervous system. (1994) Annals of Neurology. 35 Suppl:S9-12.

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Destexhe, A., Mainen, Z.F., Sejnowski, T.J. Synthesis of models for excitable membranes, synaptic transmission and neuromodulation using a common kinetic formalism. (1994) Journal of Computational Neuroscience. 1(3):195-230.

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Destexhe, A., Contreras, D., Sejnowski, T.J., Steriade, M. A model of spindle rhythmicity in the isolated thalamic reticular nucleus. (1994) Journal of Neurophysiology. 72(2):803-818.

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1. The oscillatory properties of the isolated reticular (RE) thalamus were modeled with the use of compartmental models of RE cells. Hodgkin-Huxley type kinetic models of ionic channels were derived from voltage- and current-clamp data from RE cells. Interactions between interconnected RE cells were simulated with the use of a kinetic model of gamma-aminobutyric acid (GABA) inhibitory synapses. 2. The intrinsic bursting properties of RE cells in the model were due to the presence of a low-threshold Ca2+ current and two Ca(2+)-activated currents. The properties of these model RE cells were compared with RE neurons recorded intracellularly in vivo in cats. 3. Model RE cells densely interconnected with GABAA synapses produced synchronous oscillations at a frequency close to that of spindles (7-14 Hz). Networks of RE neurons organized in a two-dimensional array with only proximal connectivity also exhibited synchronized oscillations in the spindle range. In addition, the proximally connected network showed periods of high and low synchronicity, giving rise to waxing and waning oscillations in the population of RE cells. 4. The spatiotemporal behavior of the network was investigated during waxing and waning oscillations. The waxing and waning emerged as an alternation between periods of desynchronized and synchronized activity, corresponding to periods of irregular and coherent spatial activity. During synchronized periods, the network displayed propagating coherent waves of synchronous activity that had a tendency to form spirals. 5. Networks of model RE neurons fully connected through GABAB synapses exhibited perfectly synchronous oscillations at lower frequencies (0.5-1 Hz), but two-dimensional networks with proximal GABAB connectivity failed to synchronize. 6. These simulations demonstrate that networks of model neurons that include the main intrinsic currents found in RE cells can generate waxing and waning oscillatory activity similar to the spindle rhythmicity observed in the isolated RE nucleus in vivo. The model reveals the interplay between the intrinsic rhythmic properties of RE cells and the fast synaptic interactions in organizing synchronized rhythmicity.

Ray, J., Gage, F.H. Spinal cord neuroblasts proliferate in response to basic fibroblast growth factor. (1994) Diario de Neurociencia. 14(6):3548-64.

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Trophic factors may function as one of the epigenic signals responsible for the proliferation, growth, migration, and differentiation of neurons and glia during embryogenesis. The present study reports that basic fibroblast growth factor (bFGF) at high concentrations (10-100 ng/ml) is a mitogen for embryonic spinal cord cells that have already committed to a neuronal pathway and are expressing neuronal phenotypes (neuroblasts). Neuroblasts proliferate with a doubling time of 2.5 d. To characterize the nature of cells proliferating in response to bFGF, we have established long-term cultures of neuroblasts that can be passaged, freeze thawed, and recultured. In cultures the proportion of astrocytes remained the same, indicating limited survival and proliferation of these cells in response to bFGF. These results indicate that bFGF has mitogenic effects preferably on neuroblasts. The morphological and biochemical characterizations of the neuronal populations present in the long-term neuroblast cultures are presented here. The presence of cholinergic and GABAergic neurons in the cultures was established by immunocytochemical analysis. The cultures contain a small number of motoneurons as judged by their immunostaining with ChAT, low-affinity NGF receptor (LNGFR), and large size. Among all other growth factors tested for their mitogenic effects on embryonic spinal cord cells in culture, only epidermal growth factor (EGF) showed such effects, but to a lesser degree. The proliferative nature of neuroblasts has made it possible to transduce the Escherichia coli beta-galactosidase (LacZ) gene stably into these cells in vitro using a retroviral vector. The transfected cells expressing the foreign gene can be passaged, freeze thawed, and recultured without the loss of transgenes. The ability to transduce foreign genes stably into these cells permits implantation of these cells in the spinal cord to study cellular and biochemical behaviors and gene expression in defined neuronal populations in in vivo environments.

Gage, F.H. Neuronal stem cells: their characterization and utilization. (1994) Neurobiology of Aging. 15 Suppl 2:S191.

Bush, P.C., Sejnowski, T.J. Effects of inhibition and dendritic saturation in simulated neocortical pyramidal cells. (1994) Journal of Neurophysiology. 71(6):2183-2193.

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1. We have used compartmental models of reconstructed pyramidal neurons from layers 2 and 5 of cat visual cortex to investigate the nonlinear summation of excitatory synaptic input and the effectiveness of inhibitory input in countering this excitation. 2. In simulations that match the conditions of a recent experiment, dendritic saturation was significant for physiological levels of synaptic activation: a compound excitatory postsynaptic potential (EPSP) electrically evoked during a depolarization caused by physiological synaptic activation was decreased by up to 80% compared with an EPSP evoked at rest. 3. Synaptic inhibition must be coactivated with excitation to quantitatively match the experimental results. The experimentally observed coactivation of inhibition with excitation produced additional current shunts that amplified the decrease in test EPSP amplitude. About 30% of the experimentally observed decrease in EPSP amplitude was caused by decreases in input resistance (Rin) due to synaptic conductance changes; a reduced driving force accounted for the remaining decrease. 4. The amount of inhibition was then increased by nearly an order of magnitude, to approximately 10% of the total number of inhibitory synapses on a typical cortical pyramidal cell. The sustained firing of this many inhibitory inputs was sufficient to completely suppress the firing of a neuron receiving strong excitatory input. However, this level of inhibition produced a very large reduction in Rin. Such large reductions in Rin have not been observed experimentally, suggesting that inhibition in cortex does not act to veto (shunt) strong, sustained excitatory input (of order 100 ms). 5. We propose instead that strong, transient activation (< 10 ms) of a neuron's inhibitory inputs, sufficient to briefly prevent firing, is used to shape the temporal structure of the cell's output spike train. Specifically, cortical inhibition may serve to synchronize the firing of groups of pyramidal cells during optimal stimulation.

Pouget, A., Sejnowski, T.J. A neural model of the cortical representation of egocentric distance. (1994) Cerebral Cortex. 4(3):314-329.

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Barba, D., Hardin, J., Sadelain, M., Gage, F.H. Development of anti-tumor immunity following thymidine kinase-mediated killing of experimental brain tumors. (1994) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 91(10):4348-52.

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Fisher, L.J., Gage, F.H. Intracerebral transplantation: basic and clinical applications to the neostriatum. (1994) Revista FASEB. 8(8):489-96.

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Dekker, A.J., Winkler, J., Ray, J., Thal, L.J., Gage, F.H. Grafting of nerve growth factor-producing fibroblasts reduces behavioral deficits in rats with lesions of the nucleus basalis magnocellularis. (1994) Neuroscience. 60(2):299-309.

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Rats received bilateral lesions of the nucleus basalis magnocellularis by infusion of biotenic acid. Two weeks after the lesion, a suspension of genetically modified primary rat fibroblasts was grafted dorsal to the nucleus basalis magnocellularis (2 x 10(5) cells per side). The fibroblasts were either infected with the gene for human beta-nerve growth factor or Escherichia coli beta-galactosidase. The nerve growth factor-producing fibroblasts released 67 ng nerve growth factor/10(5) cells per day in vitro. Two weeks after implantation of the fibroblasts, spatial learning was tested in the Morris water-maze. Nerve growth factor-producing fibroblasts, but not beta-galactosidase-producing fibroblasts ameliorated the deficit in acquisition of the water-maze task. In addition, spatial acuity was improved to near-normal levels by the nerve growth factor-producing grafts. Choline acetyltransferase activity in cortical areas and hippocampus was not affected by the nerve growth factor-producing grafts. Both grafted groups showed a similar reduction in the level of dopamine, but not homovanillic acid or 3-methoxytyramine, in the frontal cortex. Levels of norepinephrine, epinephrine and serotonin and their metabolites in the neocortex and hippocampus were not affected by the lesion or the grafts. Nerve growth factor-producing grafts increased the size of remaining nerve growth factor-receptor (p75) immunoreactive neurons in the nucleus basalis magnocellularis by 25%. Nucleus basalis magnocellularis lesions reduced the integrated optic density of choline acetyltransferase-positive fiber staining in the ventral neocortex by 46%, but nerve growth factor-producing grafts restored this area to 86% of control. These data suggest that nerve growth factor-producing grafts can cause a marked behavioral improvement, probably through the partial restoration of the lesioned projection from nucleus basalis magnocellularis to neocortex.

Montague, P.R., Sejnowski, T.J. The predictive brain: temporal coincidence and temporal order in synaptic learning mechanisms. (1994) Learning & Memory. 1(1):1-33.

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Goulding, M., Lumsden, A., Paquette, A.J. Regulation of Pax-3 expression in the dermomyotome and its role in muscle development. (1994) Development. 120(4):957-971.

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Chalepakis, G., Goulding, M., Read, A., Strachan, T., Gruss, P. Molecular basis of splotch and Waardenburg Pax-3 mutations. (1994) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 91(9):3685-3689.

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Beeson, J.G., Shelton, E.R., Chan, H.W., Gage, F.H. Differential distribution of amyloid protein precursor immunoreactivity in the rat brain studied by using five different antibodies. (1994) Journal of Comparative Neurology. 342(1):78-96. DOI: 10.1002/cne.903420109

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Beeson, J.G., Shelton, E.R., Chan, H.W., Gage, F.H. Age and damage induced changes in amyloid protein precursor immunohistochemistry in the rat brain. (1994) Journal of Comparative Neurology. 342(1):69-77. DOI: 10.1002/cne.903420108

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Dekker, A.J., Fagan, A.M., Gage, F.H., Thal, L.J. Effects of brain-derived neurotrophic factor and nerve growth factor on remaining neurons in the lesioned nucleus basalis magnocellularis. (1994) Brain Research. 639(1):149-55.

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Tuszynski, M.H., Peterson, D.A., Ray, J., Baird, A., Nakahara, Y., Gage, F.H. Fibroblasts genetically modified to produce nerve growth factor induce robust neuritic ingrowth after grafting to the spinal cord. (1994) Experimental Neurology. 126(1):1-14.

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Fagan, A.M., Gage, F.H. Mechanisms of sprouting in the adult central nervous system: cellular responses in areas of terminal degeneration and reinnervation in the rat hippocampus. (1994) Neuroscience. 58(4):705-25.

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Neurons of the adult mammalian central nervous system are limited in their ability to regenerate in response to injury. In certain circumstances, however, such neurons can exhibit morphological plasticity (e.g. sprouting). Unilateral transection of the perforant path in the adult rat induces terminal degeneration of entorhinal axons within the molecular layer of the ipsilateral hippocampal dentate gyrus. Cholinergic (and other) afferents subsequently sprout within the denervated zone. We show that despite the breach in the blood-brain barrier at the site of the aspirative lesion, the barrier remains intact in the areas of terminal degeneration (and reinnervation), and peripheral monocytic macrophages do not infiltrate this area to participate in the degenerative and/or regenerative events. Perforant path transection does not induce expression of major histocompatibility antigens on reactive cells within the denervated zone, nor are T lymphocytes recruited to this area. T lymphocyte-deficient Nude rats exhibit normal cholinergic sprouting. Perforant path transection does induce rapid and robust proliferation of microglia, and astrocytes to a lesser extent, in areas undergoing terminal degeneration. Histological evaluation after antimitotic administration shows that this glial proliferation is not required for the subsequent neuronal sprouting events. These results show that the reparative process in this model system involves interactions between cells endogenous to the brain in a non-immune context. Knowledge of these cellular responses provides a framework from which to further investigate putative molecular signals involved in initiating the neuronal sprouting events. Discovering the cellular and molecular interactions taking place under sprouting conditions is likely to be critical for understanding the mechanisms of reactive neuronal growth and, furthermore, may provide insights as to why regeneration is so limited in the central nervous system.

Goulding, M., Paquette, A. Pax genes and neural tube defects in the mouse. (1994) Ciba Found. Symp. 181:103-113; discussion 113-117.

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Holtzman, D.M., Li, Y., Chen, K., Gage, F.H., Epstein, C.J., Mobley, W.C. Nerve growth factor reverses neuronal atrophy in a Down syndrome model of age-related neurodegeneration. (1993) Neurology. 43(12):2668-73.

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Kang, U.J., Fisher, L.J., Joh, T.H., O'Malley, K.L., Gage, F.H. Regulation of dopamine production by genetically modified primary fibroblasts. (1993) Diario de Neurociencia. 13(12):5203-11.

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Destexhe, A., McCormick, D.A., Sejnowski, T.J. A model for 8-10 Hz spindling in interconnected thalamic relay and reticularis neurons. (1993) Biophysical Journal. 65(6):2473-2477. DOI: 10.1016/S0006-3495(93)81297-9

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Barba, D., Hardin, J., Ray, J., Gage, F.H. Thymidine kinase-mediated killing of rat brain tumors. (1993) Journal of Neurosurgery. 79(5):729-35. DOI: 10.3171/jns.1993.79.5.0729

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Gene therapy has many potential applications in central nervous system (CNS) disorders, including the selective killing of tumor cells in the brain. A rat brain tumor model was used to test the herpes simplex virus (HSV)-thymidine kinase (TK) gene for its ability to selectively kill C6 and 9L tumor cells in the brain following systemic administration of the nucleoside analog ganciclovir. The HSV-TK gene was introduced in vitro into tumor cells (C6-TK and 9L-TK), then these modified tumor cells were evaluated for their sensitivity to cell killing by ganciclovir. In a dose-response assay, both C6-TK and 9L-TK cells were 100 times more sensitive to killing by ganciclovir (median lethal dose: C6-TK, 0.1 microgram ganciclovir/ml; C6, 5.0 micrograms ganciclovir/ml) than unmodified wild-type tumor cells or cultured fibroblasts. In vivo studies confirmed the ability of intraperitoneal ganciclovir administration to kill established brain tumors in rats as quantified by both stereological assessment of brain tumor volumes and studies of animal survival over 90 days. Rats with brain tumors established by intracerebral injection of wild-type or HSV-TK modified tumor cells or by a combination of wild-type and HSV-TK-modified cells were studied with and without ganciclovir treatments. Stereological methods determined that ganciclovir treatment eliminated tumors composed of HSV-TK-modified cells while control tumors grew as expected (p < 0.001). In survival studies, all 10 rats with 9L-TK tumors treated with ganciclovir survived 90 days while all untreated rats died within 25 days. Curiously, tumors composed of combinations of 9L and 9L-TK cells could be eliminated by ganciclovir treatments even when only one-half of the tumor cells carried the HSV-TK gene. While not completely understood, this additional tumor cell killing appears to be both tumor selective and local in nature. It is concluded that HSV-TK gene therapy with ganciclovir treatment does selectively kill tumor cells in the brain and has many potential applications in CNS disorders, including the treatment of cancer.

Suhr, S.T., Gage, F.H. Gene therapy for neurologic disease. (1993) Archives of Neurology. 50(11):1252-68.

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Fisher, L.J., Schinstine, M., Salvaterra, P., Dekker, A.J., Thal, L., Gage, F.H. In vivo production and release of acetylcholine from primary fibroblasts genetically modified to express choline acetyltransferase. (1993) Journal of Neurochemistry. 61(4):1323-32.

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Steriade, M., McCormick, D.A., Sejnowski, T.J. Thalamocortical oscillations in the sleeping and aroused brain. (1993) Ciencia. 262(5134):679-685.

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Destexhe, A., Babloyantz, A., Sejnowski, T.J. Ionic mechanisms for intrinsic slow oscillations in thalamic relay neurons. (1993) Biophysical Journal. 65(4):1538-1552. DOI: 10.1016/S0006-3495(93)81190-1

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Berns, G.S., Dayan, P., Sejnowski, T.J. A correlational model for the development of disparity selectivity in visual cortex that depends on prenatal and postnatal phases. (1993) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 90(17):8277-8781.

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Fisher, L.J., Raymon, H.K., Gage, F.H. Cells engineered to produce acetylcholine: therapeutic potential for Alzheimer's disease. (1993) Annals of the New York Academy of Sciences. 695:278-84.

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Alzheimer's disease (AD) is a debilitating disorder of the central nervous system which may affect up to 50% of the population over the age of 85 years. The etiology of AD is unknown and there is currently no cure for the disease. Well-documented losses in cholinergic and other neurotransmitter systems have provided a focal point for attempting pharmacological interventions in AD to ameliorate some of the cognitive deficits that occur. However, current systemic strategies have met with limited success. An alternative strategy, that has been pursued in animal models of neurodegenerative disease, is to augment neurotransmitter function within the brain through tissue transplantation. Such implants have an advantage over conventional drug therapies in that the cells can be precisely placed within compromised areas of the brain. We have pursued a strategy of designing cells, through the use of molecular biology techniques, to produce neurotrophic factors and neurotransmitters. Recently, we developed a primary fibroblast cell line that was genetically modified to express choline acetyltransferase (ChAT). In vitro, these cells produced and released acetylcholine at levels that varied with the amount of choline in the culture media. When implanted into the hippocampus of rats, the in vivo microdialysis technique revealed that the ChAT-expressing fibroblasts continued to produce and release acetylcholine after grafting. Most importantly, the levels of acetylcholine synthesized by the cells could be regulated by the localized infusion of choline in the vicinity of the grafts. These results confirmed previous work which indicated that engineered fibroblasts provide an effective delivery vehicle of different substances to the brain. While the intracerebral implantation of genetically modified cells will not cure AD, the continuing development of this strategy may ultimately provide a powerful approach for ameliorating the devastating cognitive impairments which are a hallmark of this disease.

Callaway, E.M., Katz, L.C. Photostimulation using caged glutamate reveals functional circuitry in living brain slices. (1993) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 90(16):7661-7665.

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Goulding, M., Sterrer, S., Fleming, J., Balling, R., Nadeau, J., Moore, K.J., Brown, S.D., Steel, K.P., Gruss, P. Analysis of the Pax-3 gene in the mouse mutant splotch. (1993) Genomics. 17(2):355-363. DOI: 10.1006/geno.1993.1332

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Lockery, S.R., Sejnowski, T.J. The computational leech. (1993) Trends in Neurosciences. 16(7):283-290.

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Hammond, R.R., Gage, F.H., Terry, R.D. Alzheimer's disease and spirochetes; a questionable relationship. (1993) Neuroreport. 4(7):840.

Fisher, L.J., Gage, F.H. Grafting in the mammalian central nervous system. (1993) Physiological Reviews. 73(3):583-616.

Nilsson, O.G., Gage, F.H. Anticholinergic sensitivity in the aging rat septohippocampal system as assessed in a spatial memory task. (1993) Neurobiology of Aging. 14(5):487-97.

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Shimohama, S., Fisher, L.J., Gage, F.H. Intracerebral grafting of genetically modified cells. Applications to a rat model of Parkinson's disease. (1993) Advances in Neurology. 60:744-8.

Lockery, S.R., Sejnowski, T.J. A lower bound on the detectability of nonassociative learning in the local bending reflex of the medicinal leech. (1993) Behav. Neural Biol. 59(3):208-224.

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Yamada, T., Pfaff, S.L., Edlund, T., Jessell, T.M. Control of cell pattern in the neural tube: motor neuron induction by diffusible factors from notochord and floor plate. (1993) Móvil. 73(4):673-86.

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Schinstine, M., Gage, F.H. Factors affecting proviral expression in primary cells grafted into the CNS. (1993) Res Publ Assoc Res Nerv Ment Dis. 71:311-23.

Ray, J., Peterson, D.A., Schinstine, M., Gage, F.H. Proliferation, differentiation, and long-term culture of primary hippocampal neurons. (1993) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 90(8):3602-6.

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Pouget, A., Fisher, S.A., Sejnowski, T.J. Egocentric spaw representation in early vision. (1993) Journal of Cognitive Neuroscience. 5(2):150-161. DOI: 10.1162/jocn.1993.5.2.150

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Goulding, M.D., Lumsden, A., Gruss, P. Signals from the notochord and floor plate regulate the region-specific expression of two Pax genes in the developing spinal cord. (1993) Development. 117(3):1001-1016.

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Bush, P.C., Sejnowski, T.J. Reduced compartmental models of neocortical pyramidal cells. (1993) Journal of Neuroscience Methods. 46(2):159-166.

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Gage, F.H. Parkinson's disease. Fetal implants put to the test. (1993) Naturaleza. 361(6411):405-6. DOI: 10.1038/361405a0

Shimohama, S., Saitoh, T., Gage, F.H. Changes in protein kinase C isozymes in the rat hippocampal formation following hippocampal lesion. (1993) Hipocampo. 3(1):43-55. DOI: 10.1002/hipo.450030105

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The cellular and regional distribution of the four protein kinase C (PKC) isoforms in the rat hippocampal formation and the response of PKC to lesions were determined by employing immunohistochemical and immunochemical techniques with antibodies specific to PKC(alpha), -(beta I), -(beta II), and -(gamma). PKC(alpha) intensely stained the periphery of the pyramidal cell in the stratum pyramidale. The granule cells, glial cells, and mossy fibers were anti-PKC(alpha) negative. The cytoplasm, axons, and dendrites of basket cells and interneurons in the hilus were labeled with anti-PKC(alpha). Anti-PKC(beta I) immunoreactivity was localized on the periphery of pyramidal cells and interneurons of the hilus, as well as the oriens, radiatum, and molecular layers of the CA regions. Anti-PKC(beta II) immunoreactivity was mainly cytoplasmic, extending into the dendrites in the hippocampal pyramidal cells and the dentate granule cells, and also in some glial cells. In the stratum radiatum of the CA1, anti-PKC(gamma) immunoreactivity localized to the pyramidal cell cytoplasm, extending into the dendrites. Following fimbria-fornix (FF) lesions, the anti-PKC(alpha) and -(beta I) staining of the pyramidal cell periphery was markedly reduced. The anti-PKC(gamma) staining of the pyramidal and granular cells of the dentate gyrus was reduced whereas the interneuron staining in the hilus was increased. In the FF-lesioned hippocampus, anti-PKC(alpha) and anti-PKC(beta II) labeled reactive glial cells, whereas anti-PKC(beta I) and -(gamma) did not. Quantitative Western blot analysis revealed a dramatic increase in the particulate/total PKC for all isozyme forms, although the total levels of PKC, except PKC(gamma), did not change following FF lesions. The PKC(gamma) concentration doubled after FF lesions. Perforant path lesions resulted in a marked alteration in the neuronal staining in dentate gyrus with anti-PKC(alpha) and -(beta I) and in increased numbers of anti-PKC(alpha)- and anti-PKC(beta II)-positive glial cells. Anti-PKC(gamma) staining did not change noticeably. The total PKC concentration did not change for isozymes alpha, beta I, and gamma, but PKC (beta II) concentration increased by 48% following perforant path lesions as detected by Western blot analysis. The particulate/total PKC decreased for all four isozymes although the reduction in PKC(beta I) concentration was not statistically significant. This change in PKC compartmentalization is in marked contrast to an increased level of particulate PKC following FF lesions. Thus, the effects of deafferentation and deafferentation for each PKC isoform were different.

Chowdhury, K., Goulding, M., Walther, C., Imai, K., Fickenscher, H. The ubiquitous transactivator Zfp-38 is upregulated during spermatogenesis with differential transcription. (1992) Mech. Dev. 39(3):129-42.

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Lisberger, S.G., Sejnowski, T.J. Motor learning in a recurrent network model based on the vestibulo-ocular reflex. (1992) Naturaleza. 360(6400):159-161. DOI: 10.1038/360159a0

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Lockery, S.R., Sejnowski, T.J. Distributed processing of sensory information in the leech. III. A dynamical neural network model of the local bending reflex. (1992) Diario de Neurociencia. 12(10):3877-3895.

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Ray, J., Gage, F.H. Gene transfer into established and primary fibroblast cell lines: comparison of transfection methods and promoters. (1992) BioTechniques. 13(4):598-603.

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Fischer, W., Chen, K.S., Gage, F.H., Björklund, A. Progressive decline in spatial learning and integrity of forebrain cholinergic neurons in rats during aging. (1992) Neurobiology of Aging. 13(1):9-23.

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Rats distributed over five different age groups, 3, 12, 18, 24 and 30 months of age, were screened for their spatial learning and memory ability in the Morris water maze, and the degree of place navigational impairments was correlated with morphological changes in the four major forebrain cholinergic cell groups (medial septum, MS; vertical limb of the diagonal band of Broca, VDB; nucleus basalis magnocellularis, NBM; and striatum) using choline acetyltransferase (ChAT) and nerve growth factor receptor (NGFr) histochemistry. Impaired place navigation developed progressively with age, such that 8% of the 12-month-old rats, 45% of the 18-month-old, 53% of the 24-month-old, and over 90% of the 30-month-old rats were behaviorally impaired. Significant reductions in the number of ChAT/NGFr-positive cell bodies, amounting to between 19 and 45%, were observed in all four cell groups, and the remaining cells were reduced in size (6-24% reduction in cross-sectional area in the oldest age groups). Although the morphological changes were less severe and tended to develop later than the behavioral impairments, there was overall a significant correlation between water maze performance and ChAT/NGFr-positive cell counts, and to a lesser degree also cell size in all four cell groups. These changes were also highly correlated with age. The highest correlations were seen in MS, VDB and NBM, which are known to play a role in spatial memory performance in young rats. The results indicate that degenerative and/or atrophic changes in the forebrain cholinergic system and decline in spatial learning ability are parallel processes during aging. Although the magnitude of the morphological changes does not appear to be substantial enough, by itself, to explain the severe spatial learning impairments that develop in the oldest animals, the present data are consistent with the view that impaired function in the forebrain cholinergic system can contribute to age-dependent cognitive decline in rodents.

Lehky, S.R., Sejnowski, T.J., Desimone, R. Predicting responses of nonlinear neurons in monkey striate cortex to complex patterns. (1992) Diario de Neurociencia. 12(9):3568-3581.

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Yoshida, K., Kakihana, M., Chen, L.S., Ong, M., Baird, A., Gage, F.H. Cytokine regulation of nerve growth factor-mediated cholinergic neurotrophic activity synthesized by astrocytes and fibroblasts. (1992) Journal of Neurochemistry. 59(3):919-31.

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The neurotrophic activity of astrocytes and fibroblasts and its regulation by various cytokines were investigated. Astrocyte conditioned medium (ACM) enhanced the survival of neurons and the proliferation of astrocytes in embryonic cortical cultures grown in serum-free defined medium. However, these results were not affected by acidic fibroblast growth factor, interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF alpha), and transforming growth factor-beta 1. In contrast, ACM induced choline acetyltransferase expression in septal cholinergic neurons via nerve growth factor (NGF)-dependent and -independent mechanisms. However, neither acidic nor basic fibroblast growth factor is involved in this biological activity in ACM. The cytokines listed above mainly stimulate NGF-mediated cholinergic neurotrophic activity in ACM. A combination of IL-1 beta and TNF alpha significantly enhanced choline acetyltransferase activity in septal neurons co-cultured with astrocytes, and this effect was found to be mediated by NGF produced by activated astrocytes. Effects of astrocytes on GABAergic neurons were also examined. ACM was found to increase glutamate decarboxylase activity in neuronal cultures from septum in the presence of Ara-C. However, the cytokines did not enhance this activity in ACM. Moreover, a combination of IL-1 beta and TNF alpha had no effect on glutamate decarboxylase activity in septal neurons co-cultured with astrocytes. In a final set of experiments, cholinergic neurotrophic activity in skin-derived fibroblast conditioned medium (FCM) was examined. FCM was found to possess biological activity similar to that of ACM on septal neurons grown in serum-free defined medium with Ara-C. The cytokines also enhanced NGF-mediated cholinergic neurotrophic activity in FCM. Astrocytes and fibroblasts were found to possess NGF-type and non-NGF-type cholinergic neurotrophic activity, and various cytokines were found to regulate the NGF-type cholinergic neurotrophic activity in both types of cells. NGF produced by astrocytes and fibroblasts that are activated by cytokines is likely to be important for development and regeneration of NGF-sensitive neurons in the central and peripheral nervous systems.

Thal, L.J., Gilbertson, E., Armstrong, D.M., Gage, F.H. Development of the basal forebrain cholinergic system: phenotype expression prior to target innervation. (1992) Neurobiology of Aging. 13(1):67-72.

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Holtzman, D.M., Li, Y., Gage, F.H., Epstein, C.J., Mobley, W.C. Neurodegeneration and gene dysregulation: studies utilizing the mouse trisomy 16 model of Down syndrome. (1992) Progress in Clinical and Biological Research. 379:227-44.

Lytton, W.W., Sejnowski, T.J. Computer model of ethosuximide's effect on a thalamic neuron. (1992) Ann. Neurol. 32(2):131-139. DOI: 10.1002/ana.410320204

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Sejnowski, T.J. Models of vision. (1992) Ciencia. 257(5070):687-688. DOI: 10.1126/science.257.5070.687

Kawaja, M.D., Rosenberg, M.B., Yoshida, K., Gage, F.H. Somatic gene transfer of nerve growth factor promotes the survival of axotomized septal neurons and the regeneration of their axons in adult rats. (1992) Diario de Neurociencia. 12(7):2849-64.

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Katz, L.C., Callaway, E.M. Development of local circuits in mammalian visual cortex. (1992) Revisión Anual de Neurociencia. 15:31-56. DOI: 10.1146/annurev.ne.15.030192.000335

Schinstine, M., Rosenberg, M.B., Routledge-Ward, C., Friedmann, T., Gage, F.H. Effects of choline and quiescence on Drosophila choline acetyltransferase expression and acetylcholine production by transduced rat fibroblasts. (1992) Journal of Neurochemistry. 58(6):2019-29.

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Pfaff, S.L., Taylor, W.L. Characterization of a Xenopus oocyte factor that binds to a developmentally regulated cis-element in the TFIIIA gene. (1992) Developmental Biology. 151(1):306-16.

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Gage, F.H. Repopulating the mortal brain with immortal cells. (1992) Biología Actual. 2(5):232-4.

Lyden, P.D., Zivin, J.A., Chabolla, D.R., Jacobs, M.A., Gage, F.H. Quantitative effects of cerebral infarction on spatial learning in rats. (1992) Experimental Neurology. 116(2):122-32.

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Kawaja, M.D., Gage, F.H. Morphological and neurochemical features of cultured primary skin fibroblasts of Fischer 344 rats following striatal implantation. (1992) Journal of Comparative Neurology. 317(1):102-16. DOI: 10.1002/cne.903170108

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In order to assess the feasibility of using primary skin fibroblasts as a donor population for genetic modification and subsequent intracerebral grafting, the present study examines the structural and neurochemical characteristics of intrastriatal grafts of isogeneic primary fibroblasts over a period of 6 months. In culture, primary skin fibroblasts obtained from a female Fischer 344 rat display robust growth, but once confluent these cells exhibit contact inhibition. Following the implantation of cultured primary cells within the striatum of other adult rats from the same inbred strain, isologous grafts stain immunohistochemically for fibronectin at 1 week, and this immunostaining persists up to 6 months. Immunoreactivity for laminin is intense within the grafts from 1 to 8 weeks, but decreases by 6 months. Astrocytes within the striatum respond dramatically to the implantation of primary fibroblasts, such that immunohistochemical staining for glial fibrillary acidic protein increases markedly from 1 to 8 weeks after implantation. Although the intensity of immunostaining for glial fibrillary acidic protein diminishes among striatal astrocytes between 8 weeks and 6 months, the astrocytic border between the grafts and striatal neuropil remains intensely immunoreactive. Capillaries within the grafts stain immunohistochemically for glucose transporter (a facilitated glucose uptake carrier) as early as 3 weeks after implantation. Following intravenous infusions of peroxidase, capillaries within fibroblast grafts do not permit the extravasation of this macromolecule at 8 weeks and 6 months. Thus, capillaries formed within intracerebral grafts of primary skin fibroblasts exhibit a functional impermeable barrier to macromolecules similar to those capillaries of the host striatum. At the ultrastructural level, grafts possess numerous fibroblasts and have an extracellular matrix filled with collagen. Reactive astrocytic processes filled with intermediate filaments are found throughout the grafts. Hypertrophied astrocytes and their processes also appear to form a continuous border between the grafts and striatal neuropil. Grafts of primary fibroblasts also possess an extensive vasculature that is composed of capillaries with nonfenestrated endothelial cells; the occurrence of reactive astrocytic processes closely associated with or enveloping capillaries is variable. These results provide direct morphological and neurochemical evidence for the long-term survival of isologous fibroblasts after implantation within the rat striatum. From these data, we propose that isologous skin fibroblasts can be considered as donor candidates for successful intracerebral grafting following gene transfer.

Buzsáki, G., Gage, F.H. Fetal brain tissue grafts modulate neuronal excitability in a chronic model of epilepsy. (1992) Epilepsy Res. Suppl. 8:271-81.

Wathey, J.C., Lytton, W.W., Jester, J.M., Sejnowski, T.J. Computer simulations of EPSP-spike (E-S) potentiation in hippocampal CA1 pyramidal cells. (1992) Diario de Neurociencia. 12(2):607-618.

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Callaway, E.M., Katz, L.C. Development of axonal arbors of layer 4 spiny neurons in cat striate cortex. (1992) Diario de Neurociencia. 12(2):570-582.

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Spiny neurons in layer 4 of cat striate cortex are the primary recipients of geniculocortical afferents and provide crucial links to other cortical layers for processing visual information. Using intracellular staining, we examined the development of the local axonal projections of these neurons to determine (1) whether the laminar specificity of their projections emerged specifically or was sculpted from transient exuberant projections and (2) whether the emergence of excitatory connections from layer 4 to layer 2/3 could contribute to the activity-dependent development of clustered horizontal connections of layer 2/3 pyramidal neurons. Differences in the extent of projections to infragranular (layers 5 and 6, which receive sparse projections) versus superficial layers (layers 2/3 and 4, which receive extensive projections) developed specifically from the outset. By postnatal day 15 (P15) projections to infragranular layers matured and were indistinguishable from those in the oldest animal studied (P33). In contrast, projections to superficial layers continued to increase in complexity after P15. Projections within layer 4, which were the most elaborate at all ages studied, reached maturity at about P20, while those to layer 2/3 continued to increase in complexity through P33. No evidence for exuberant projections to any of these cortical layers was observed. At very early postnatal ages (P5) projections to the subplate region were evident. These disappeared by P8-P11, suggesting the presence of transient connections from layer 4 spiny neurons to subplate neurons. Binocular deprivation did not prevent the emergence of projections from layer 4 spiny neurons into layer 2/3 or development of normal laminar differences in projection density. Connections from layer 4 to layer 2/3 emerged after horizontal connections in layer 2/3 were crudely clustered, but in synchrony with the later refinement of clusters. Collaterals from layer 4 cells first crossed into layer 3 at P11, but were extremely short (extending only 50-200 microns beyond the laminar boundary) and uncommon (only 4 of 19 cells). Since by P8 horizontal projections of layer 2/3 pyramidal neurons are already crudely clustered, the emergence of crude clustering is probably independent of layer 4 to layer 2/3 excitatory projections. The proportion of cells projecting to layer 2/3 and the complexity of their arbors both increased in the subsequent weeks, closely matching the timing of both the refinement of crudely clustered horizontal connections and the emergence of visual responsiveness in layer 2/3.(ABSTRACT TRUNCATED AT 400 WORDS)

Holtzman, D.M., Li, Y.W., DeArmond, S.J., McKinley, M.P., Gage, F.H., Epstein, C.J., Mobley, W.C. Mouse model of neurodegeneration: atrophy of basal forebrain cholinergic neurons in trisomy 16 transplants. (1992) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 89(4):1383-7.

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Jinnah, H.A., Hess, E.J., Wilson, M.C., Gage, F.H., Friedmann, T. Localization of hypoxanthine-guanine phosphoribosyltransferase mRNA in the mouse brain by in situ hybridization. (1992) Molecular and Cellular Neurosciences. 3(1):64-78.

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Gage, F.H., Chen, K.S. Neural transplants: prospects for Alzheimer's disease. (1992) Current Opinion in Neurology and Neurosurgery. 5(1):94-9.

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Dekker, A.J., Gage, F.H., Thal, L.J. Delayed treatment with nerve growth factor improves acquisition of a spatial task in rats with lesions of the nucleus basalis magnocellularis: evaluation of the involvement of different neurotransmitt (1992) Neuroscience. 48(1):111-9.

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Yoshida, K., Gage, F.H. Cooperative regulation of nerve growth factor synthesis and secretion in fibroblasts and astrocytes by fibroblast growth factor and other cytokines. (1992) Brain Research. 569(1):14-25.

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Acidic fibroblast growth factor (aFGF) enhances nerve growth factor (NGF) synthesis by astrocytes obtained from various brain regions. NGF secretion by fibrous-shaped astrocytes transformed by dibutyryl-cAMP (db-cAMP) pretreatment was less than that by untreated astrocytes. However, aFGF also enhanced NGF secretion by fibrous-shaped astrocytes. The effects of various kinds of intracellular signaling modulators on NGF synthesis were examined. None of the following second messenger effectors had an effect on NGF synthesis: protein kinase C (PKC) agonist (phorbol myristate acetate (PMA)) or antagonist (sphingosine (SP)). LiCl, and ionomycin (Iono). Further, increases of intracellular cAMP by forskolin (FK) or db-cAMP have no significant effect on NGF synthesis in astrocytes under a standard culture condition. However, NGF synthesis by astrocytes in the presence of aFGF was significantly enhanced by db-cAMP, but not by FK or sodium butyrate. These results indicate that an excessive amount of cAMP enhances the effect of aFGF on NGF synthesis in astrocytes. NGF synthesis in astrocytes was not affected by treatment with anti-aFGF or anti-bFGF neutralizing antibodies, indicating that FGFs are not involved in the autocrine regulation of NGF synthesis in astrocytes. Transforming growth factor-beta 1 (TGF-beta 1), which inhibits some effects of FGFs, increased NGF synthesis in concert with aFGF. Furthermore, the highest NGF synthesis was observed when astrocytes were stimulated by all of the following cytokines: aFGF, interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and TGF-beta 1. The mechanism regulating NGF synthesis in fibroblasts obtained from prenatal rat skin was also investigated. Acidic FGF, basic FGF (bFGF), epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factor-alpha (TGF-alpha), TGF-beta 1, IL-1 beta, and TNF-alpha were found to be regulators of NGF synthesis in skin fibroblasts. Among these cytokines, aFGF is the most potent regulator of NGF synthesis in fibroblasts. NGF synthesis by skin fibroblasts, either in the presence or absence of aFGF, was not modified by any of the following: FK, PMA, SP, LiCl, and Iono. However, db-cAMP significantly enhanced NGF synthesis in both conditions. Sodium butyrate enhanced NGF synthesis in the presence of aFGF, but not in the absence of aFGF. These results suggest that an excessive amount of cAMP and butyrate moiety regulate NGF synthesis in skin fibroblasts in different ways.(ABSTRACT TRUNCATED AT 400 WORDS)

Buzsáki, G., Hsu, M., Horváth, Z., Gage, F.H. Physiological and behavioral consequences of delayed septal grafts in the subcortically denervated hippocampus. (1992) Restor. Neurol. Neurosci. 4(6):369-80. DOI: 10.3233/RNN-1992-4602

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Johnson, P.A., Yoshida, K., Gage, F.H., Friedmann, T. Effects of gene transfer into cultured CNS neurons with a replication-defective herpes simplex virus type 1 vector. (1992) Brain Research. Molecular Brain Research. 12(1-3):95-102.

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Jinnah, H.A., Gage, F.H., Friedmann, T. Amphetamine-induced behavioral phenotype in a hypoxanthine-guanine phosphoribosyltransferase-deficient mouse model of Lesch-Nyhan syndrome. (1991) Behavioral Neuroscience. 105(6):1004-12.

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Kawaja, M.D., Gage, F.H. Reactive astrocytes are substrates for the growth of adult CNS axons in the presence of elevated levels of nerve growth factor. (1991) Neurona. 7(6):1019-30.

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Holliday, J., Adams, R.J., Sejnowski, T.J., Spitzer, N.C. Calcium-induced release of calcium regulates differentiation of cultured spinal neurons. (1991) Neurona. 7(5):787-796.

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Tuszynski, M.H., Sang, H., Yoshida, K., Gage, F.H. Recombinant human nerve growth factor infusions prevent cholinergic neuronal degeneration in the adult primate brain. (1991) Annals of Neurology. 30(5):625-36. DOI: 10.1002/ana.410300502

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Kawaja, M.D., Ray, J., Gage, F.H. Employment of fibroblasts for gene transfer: applications for grafting into the central nervous system. (1991) Genetic Engineering. 13:205-20.

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Walther, C., Guenet, J.L., Simon, D., Deutsch, U., Jostes, B., Goulding, M.D., Plachov, D., Balling, R., Gruss, P. Pax: a murine multigene family of paired box-containing genes. (1991) Genomics. 11(2):424-434.

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Gage, F.H., Kang, U.J., Fisher, L.J. Intracerebral grafting in the dopaminergic system: issues and controversy. (1991) Current Opinion in Neurobiology. 1(3):414-9.

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Dekker, A.J., Langdon, D.J., Gage, F.H., Thal, L.J. NGF increases cortical acetylcholine release in rats with lesions of the nucleus basalis. (1991) Neuroreport. 2(10):577-80.

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Schinstine, M., Kawaja, M.D., Gage, F.H. Intracerebral delivery of growth factors: potential application of genetically modified fibroblasts. (1991) Progress in Growth Factor Research. 3(1):57-66.

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Lytton, W.W., Sejnowski, T.J. Simulations of cortical pyramidal neurons synchronized by inhibitory interneurons. (1991) Journal of Neurophysiology. 66(3):1059-1079.

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1. The interaction between inhibitory interneurons and cortical pyramidal neurons was studied by use of computer simulations to test whether inhibitory interneurons could assist in phase-locking postsynaptic cells. Two models were used: a simplified model, which included only 3 membrane channels, and a detailed 11-channel model. 2. The 11-channel model included most of the ion channels known to be present in neocortical pyramidal neurons as well as calcium diffusion and other membrane mechanisms. The kinetics for the channels were obtained from voltage-clamp studies in a variety of preparations. The parameters were then adjusted to produce repetitive bursting similar to that seen in some cortical pyramidal cells entrained during visual stimulation. 3. Phase-locking to a train of inhibitory postsynaptic potentials (IPSPs) located on or near the soma was observed in the 3-channel model cell subjected to random synaptic bombardment. In the 11-channel model, phase-locking due to multiple IPSPs was compared with phase-locking due to multiple excitatory postsynaptic potentials (EPSPs). Phase-locking began to occur when 20% of the IPSPs (20/100) or 40% of the EPSPs (4,000/10,000) were synchronized. The exact percentages differed with different 11-channel models, but either EPSPs or IPSPs would generally produce entrainment with approximately 40% synchronization. Thus 40 inhibitory boutons had an effect equivalent to 4,000 excitatory boutons in producing phase-locking. 4. Phase-locking with IPSPs in these models was possible because the IPSPs could cause either an increase or a decrease in firing rate over a limited range. The IPSPs served a modulatory role, increasing the rate of firing in some cases and decreasing it in others, depending on the state of the cell. 5. We examined frequency entrainment by IPSPs. In the 3-channel model, frequency entrainment of a postsynaptic cell was observed with a rapid train of strong (20-100 nS), brief, compound IPSPs. A 40-Hz compound IPSP train of 60 nS entrained cells having initial firing rates between 32 and 47 Hz. Below this range, cells could be partially entrained. Above the range, entrainment would fail. Frequency entrainment in the 3-channel model generally occurred on the first cycle after onset of the IPSPs. 6. Phase-locking and frequency entrainment were less robust in the 11-channel model. This was partly because bursts rather than individual spikes were being entrained. A 40-Hz, 90-nS compound IPSP train entrained a model cell upward from 34 Hz. Downward frequency entrainment also occurred.(ABSTRACT TRUNCATED AT 400 WORDS)

Chalepakis, G., Fritsch, R., Fickenscher, H., Deutsch, U., Goulding, M., Gruss, P. The molecular basis of the undulated/Pax-1 mutation. (1991) Móvil. 66(5):873-884.

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Bush, P.C., Sejnowski, T.J. Simulations of a Reconstructed Cerebellar Purkinje Cell Based on Simplified Channel Kinetics. (1991) Computación neuronal. 3(3):321-332. DOI: 10.1162/neco.1991.3.3.321

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Sejnowski, T.J. Neuroscience. Back together again. (1991) Naturaleza. 352(6337):669-670. DOI: 10.1038/352669a0

Masliah, E., Fagan, A.M., Terry, R.D., DeTeresa, R., Mallory, M., Gage, F.H. Reactive synaptogenesis assessed by synaptophysin immunoreactivity is associated with GAP-43 in the dentate gyrus of the adult rat. (1991) Experimental Neurology. 113(2):131-42.

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Reactive synaptogenesis and terminal proliferation are known to occur in the dentate gyrus of the rat hippocampus following removal of specific afferents. In the present study we have examined the relation of synaptophysin immunoreactivity to the immunohistochemical staining pattern of GAP-43, a putative marker of neuritic growth. Within the molecular layer of the normal dentate gyrus, synaptophysin immunolabeling shows a trilaminar pattern, with the inner and outer layers having the greatest density of staining. Within the first week following denervation, there was a significant decrease in the staining density in the outer two-thirds of the molecular layer, followed by a moderate recovery at 14 days and 80% recovery by 30 days. This pattern is consistent with the time course of denervation and reinnervation in this system as determined previously by electron microscopy. By comparison, the staining pattern for GAP-43 in the intact dentate gyrus showed the middle and outer thirds of the molecular layer to be less densely stained than the inner third. Within a week following deafferentation, the outer two-thirds of the molecular layer displayed decreased levels of GAP-43 immunoreactivity, followed by recovery to normal levels by 30 days. By 84 days postlesion, patterns of both synaptophysin and GAP-43 immunostaining reflected an increased width of the inner molecular layer. Laser confocal imaging of double-immunolabeled sections at 14 days postlesion showed a 370% increase in the number of GAP-43-positive terminals in the molecular layer as compared to unoperated controls. Many of these GAP 43-positive terminals were synaptophysin negative. We conclude that GAP-43 may play a role in the synaptic remodeling that occurs in the denervated rat hippocampus and that quantitative morphometry of synaptophysin immunolabeling accurately reflects the fate of presynaptic terminals in this model of degeneration and reinnervation.

Gage, F.H., Kawaja, M.D., Fisher, L.J. Genetically modified cells: applications for intracerebral grafting. (1991) Trends in Neurosciences. 14(8):328-33.

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Buzsáki, G., Masliah, E., Chen, L.S., Horváth, Z., Terry, R., Gage, F.H. Hippocampal grafts into the intact brain induce epileptic patterns. (1991) Brain Research. 554(1-2):30-7.

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Carman, L.S., Gage, F.H., Shults, C.W. Partial lesion of the substantia nigra: relation between extent of lesion and rotational behavior. (1991) Brain Research. 553(2):275-83.

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Fischer, W., Björklund, A., Chen, K., Gage, F.H. NGF improves spatial memory in aged rodents as a function of age. (1991) Diario de Neurociencia. 11(7):1889-906.

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Pfaff, S.L., Hall, R.K., Hart, G.C., Taylor, W.L. Regulation of the Xenopus laevis transcription factor IIIA gene during oogenesis and early embryogenesis: negative elements repress the O-TFIIIA promoter in embryonic cells. (1991) Developmental Biology. 145(2):241-54.

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Stanton, P.K., Chattarji, S., Sejnowski, T.J. 2-Amino-3-phosphonopropionic acid, an inhibitor of glutamate-stimulated phosphoinositide turnover, blocks induction of homosynaptic long-term depression, but not potentiation, in rat hippocampus. (1991) Neurosci. Lett. 127(1):61-66.

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Gage, F.H., Tuszynski, M.H., Chen, K.S., Fagan, A.M., Higgins, G.A. Nerve growth factor function in the central nervous system. (1991) Current Topics in Microbiology and Immunology. 165:71-93.

Holtzman, D.M., Li, Y.W., Gage, F.H., DeArmond, S.J., Epstein, C.J., McKinley, M.P., Mobley, W.C. Modeling cholinergic abnormalities in Down syndrome and Alzheimer's disease. (1991) Progress in Clinical and Biological Research. 373:189-202.

Gonzalez, A.M., Lappi, D.A., Buscaglia, M.L., Carman, L.S., Gage, F.H., Baird, A. Basic FGF-SAP mitotoxin in the hippocampus. Specific lethal effect on cells expressing the basic FGF receptor. (1991) Annals of the New York Academy of Sciences. 638:442-4.

Goulding, M.D., Chalepakis, G., Deutsch, U., Erselius, J.R., Gruss, P. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. (1991) Revista EMBO. 10(5):1135-1147.

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Kawaja, M.D., Fagan, A.M., Firestein, B.L., Gage, F.H. Intracerebral grafting of cultured autologous skin fibroblasts into the rat striatum: an assessment of graft size and ultrastructure. (1991) Journal of Comparative Neurology. 307(4):695-706. DOI: 10.1002/cne.903070414

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Masliah, E., Yoshida, K., Shimohama, S., Gage, F.H., Saitoh, T. Differential expression of protein kinase C isozymes in rat glial cell cultures. (1991) Brain Research. 549(1):106-11.

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Kawaja, M.D., Gage, F.H. Nerve growth factor receptor immunoreactivity in the rat septohippocampal pathway: a light and electron microscope investigation. (1991) J. Comp. Neurol. 307(3):517-29. DOI: 10.1002/cne.903070313

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Nerve growth factor receptor immunoreactivity in the septohippocampal pathway of adult Fischer 344 rats was assessed at the light and electron microscope level. The medial septum possesses immunoreactive somata, dendrites, axons, and terminals. Immunostained somata are either bipolar or multipolar in appearance. Dendritic processes of immunoreactive septal neurons are categorized into two groups: proximal dendrites with smooth plasma membranes and distal dendrites with numerous swellings. Immunoreactive axons within the septum are long and slender and do not possess varicosities. At the electron microscope level, immunoreactivity is confined predominantly to the plasma membrane of cell bodies and dendrites of septal neurons, as well as to the plasma membrane of axons and terminals. Both immunoreactive and nonimmunoreactive terminals that contain clear, spherical vesicles are observed contacting immunoreactive dendrites and somata. Although accumulations of vesicles are evident within these terminals at sites of contact, distinct synaptic specializations are difficult to distinguish due to the localization of reaction product on the apposing plasma membranes. Axons possessing immunoreactivity are also observed in the fimbria-fornix pathway, a major source of afferent inputs to the hippocampus. Immunoreactive axons and terminals are topographically organized in the hippocampal dentate gyrus. The density of immunostained axons and terminals is highest immediately adjacent to the granular layer. In comparison, a moderate density of immunoreactive axons is found in the outer molecular layer and a weak density in the inner molecular, granular, and polymorphic layers. Immunoreactivity is found on the plasma membrane of small unmyelinated axons and terminals aggregated into clusters throughout the dentate gyrus. Definitive examples of axosomatic and axodendritic synapses possessing immunoreactivity presynaptically are not observed. Immunoreactive profiles within the medial septum and hippocampus also circumfuse a small number of intracerebral vessels. Ultrastructural examination reveals that immunoreactivity is present within a narrowed extension of the subarachnoid space and appears to be closely associated with the plasma membrane of leptomeningeal cell processes. The present study provides direct evidence for the cellular distribution of nerve growth factor receptor immunoreactivity in the medial septum and dentate gyrus in the adult rat and offers new insight into the ultrastructural localization of nerve growth factor receptor among septal cholinergic neurons and their efferent projections to the hippocampus.

Tuszynski, M.H., Gage, F.H. In vivo assay of neuron-specific effects of nerve growth factor. (1991) Methods in Enzymology. 198:35-48.

Buzsáki, G., Hsu, M., Slamka, C., Gage, F.H., Horváth, Z. Emergence and propagation of interictal spikes in the subcortically denervated hippocampus. (1991) Hipocampo. 1(2):163-80. DOI: 10.1002/hipo.450010205

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Spontaneous and evoked field potentials and cellular discharges of the subcortically denervated dorsal hippocampus were studied by multisite recordings in the freely behaving rat. Characteristic short-duration (< 100 ms), large-amplitude (up to 10 mV) transients, termed interictal spikes (IIS), were seen after fimbria-fornix (FF) lesion. Both pyramidal cells and putative interneurons fired maximally during IIS, with some interneurons sustaining long bursts (up to 400 ms) of high-frequency discharges (400-600 Hz) after the IIS. The speed of propagation of IIS along the longitudinal axis of the hippocampus varied from 0.2 m/s to > 3 m/s. The majority of IIS (type 1) could be accounted for by an enhanced activity of the intrahippocampal associational systems; a second class of IIS (type 2) had positive polarities in the stratum radiatum of CA1 and CA3 and propagated very rapidly (> 1.5 m/s). The authors propose that type 2 IIS reflect somatic depolarization and discharge of pyramidal neurons due to nonsynaptic (probably ephaptic) effects. Ephaptic interactions may also explain the longitudinal propagation of IIS at speeds higher than the conduction velocities (0.5 m/s) of hippocampal fiber systems. IIS emerged during the first 3 weeks after fimbria-fornix lesion, their incidence reaching a plateau of 2/min thereafter. During the same time period, paired-pulse suppression increased in the dentate gyrus. The amplitude of test responses to angular bundle stimulation was potentiated by small-amplitude IIS but suppressed by large-amplitude IIS. The incidence of IIS was significantly suppressed during walking relative to standing still. Tetanic stimulation of the angular bundle or handling-induced stress resulted in a 10- to 20-fold increase in the incidence of IIS that lasted for about 30 minutes. There was a negative correlation between evoked field PSP slope and population spike amplitude in the dentate gyrus of FF-lesioned rats; this correlation was positive in intact rats. The authors attribute the above pathophysiological changes to sprouting of both excitatory and inhibitory GABAergic pathways as a result of denervation of the intrahippocampal circuitry. They hypothesize that the majority of the observed physiological alterations can be traced to a weakening of feedforward inhibition coupled with an enhancement of feedback inhibition and excitation.

Chen, L.S., Ray, J., Fisher, L.J., Kawaja, M.D., Schinstine, M., Kang, U.J., Gage, F.H. Cellular replacement therapy for neurologic disorders: potential of genetically engineered cells. (1991) Journal of Cellular Biochemistry. 45(3):252-7. DOI: 10.1002/jcb.240450305

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Shults, C.W., O'Connor, D.T., Baird, A., Hill, R., Goetz, C.G., Watts, R.L., Klawans, H.L., Carvey, P.M., Bakay, R.A., Gage, F.H. Clinical improvement in parkinsonian patients undergoing adrenal to caudate transplantation is not reflected by chromogranin A or basic fibroblast growth factor in ventricular fluid. (1991) Exp. Neurol. 111(3):276-81.

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Dawson, T.M., Dawson, V.L., Gage, F.H., Fisher, L.J., Hunt, M.A., Wamsley, J.K. Functional recovery of supersensitive dopamine receptors after intrastriatal grafts of fetal substantia nigra. (1991) Experimental Neurology. 111(3):282-92.

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Interruption of the ascending dopamine neurons of the nigrostriatal pathway, by 6-hydroxydopamine (6-OHDA) lesion in rats, produced a significant loss of the dopamine transport complexes labeled with the phencyclidine derivative [3H]BTCP. This loss of dopamine innervation in the striatum was present at least 12 to 14 months after lesioning and was functionally manifested by ipsilateral rotation of the animals in response to amphetamine. In these same animals, in comparison to controls, there was a significant increase in the number (Bmax) of [3H]SCH 23390-labeled D-1 receptors in the striatum (36.7%) and the substantia nigra (35.1%) and a 54.4% increase in the number (Bmax) of [3H]sulpiride-labeled striatal D-2 receptors without an apparent change in affinity (Kd). Ten to twelve months after the transplantation of homologous fetal substantia nigra into the denervated striatum, there was a significant decrease in amphetamine-induced turning behavior. In these animals, there was an ingrowth of dopamine nerve terminals in the striatum as demonstrated by a return of [3H]BTCP binding. Accompanying this reinnervation was the normalization of D-1 and D-2 receptors to control values in the striatum as well as the return of D-1 receptors to prelesion densities in the substantia nigra. In a subgroup of transplanted rats, amphetamine continued to induce ipsilateral turning. In these animals both D-1 and D-2 receptors remained supersensitive. These results support the hypothesis that the functional recovery of transplanted animals is due, in part, to reinnervation of the striatum. In addition, long-term alterations in receptor density may be related to the behavioral deficits that are associated with the 6-OHDA-lesioned rat. Furthermore, dopamine receptor plasticity may play a role in the functional recovery of substantia nigra transplanted animals and graft viability seems to be a prerequisite for behavioral recovery as well as receptor normalization.

Fisher, L.J., Jinnah, H.A., Kale, L.C., Higgins, G.A., Gage, F.H. Survival and function of intrastriatally grafted primary fibroblasts genetically modified to produce L-dopa. (1991) Neurona. 6(3):371-80.

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Fisher, L.J., Young, S.J., Tepper, J.M., Groves, P.M., Gage, F.H. Electrophysiological characteristics of cells within mesencephalon suspension grafts. (1991) Neuroscience. 40(1):109-22.

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Both spontaneous and evoked extracellular electrophysiological activity of neurons within fetal mesencephalon suspension grafts to the dopamine-depleted striatum of rats were examined. In some cases, extracellular recording was combined with intracellular labeling to identify recorded neurons. Grafted rats displaying a complete cessation of ipsilateral rotations following amphetamine administration were examined at post-implantation time intervals of two, four, five, eight and nine months. Four separate classes of neurons were distinguished within the transplanted striatum based on electrophysiological properties. The first of these groups, the type I cells, appeared to be non-grafted striatal neurons. When spontaneously active, these striatal-like cells fired bursts of action potentials separated by periods of decreased activity. Evoked responses in these cells were characteristic of striatal cells. Type I cells which were intracellularly labeled were found outside the grafts and displayed the characteristic morphology of the medium spiny neuron of the neostriatum. The other three cell classes displayed electrophysiological properties similar to neurons recorded in situ within the reticular formation, substantia nigra pars compacta and substantia nigra pars reticulata. Neurons from these three groups which were labeled with an intracellular marker were found to lie within the suspension grafts. The spontaneous activity of the pars compacta dopaminergic-like neurons was predominantly irregular, with some cells also firing in a regular or pacemaker-like pattern. Infrequently, irregular firing dopaminergic-like neurons displayed episodes of doublet bursting. Many of the grafted neurons responded to electrical stimulation of prefrontal cortex and striatum, indicating that the graft was receiving functional inputs from host neurons. Comparison of the firing rate and pattern of grafted neurons to in situ mesencephalic neurons as a function of time following grafting suggested that the grafted neurons and/or the neuronal circuitry is slowly developing within the host environment. A prolonged time-course for the maturation of the graft may be reflected in the time required to achieve improvements in some behavioral deficits following transplantation. However, the relatively rapid recovery of drug-induced rotational asymmetry following grafting suggests that this form of recovery may not require mature functioning of the grafted neurons.

Wamsley, J.K., Dawson, T.M., Dawson, V.L., Hunt, M.A., Fisher, L.J., Gage, F.H. Reversal of nigrostriatal-lesion-induced receptor alterations by grafting of fetal mesencephalic dopaminergic neurons. (1991) Advances in Experimental Medicine and Biology. 287:221-35.

Sejnowski, T.J. Synaptic mechanisms for long-term depression. (1991) Biología Actual. 1(1):38-40.

Dawson, T.M., Dawson, V.L., Gage, F.H., Fisher, L.J., Hunt, M.A., Wamsley, J.K. Downregulation of muscarinic receptors in the rat caudate-putamen after lesioning of the ipsilateral nigrostriatal dopamine pathway with 6-hydroxydopamine (6-OHDA): normalization by fetal mesencephali (1991) Brain Research. 540(1-2):145-52.

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Yoshida, K., Gage, F.H. Fibroblast growth factors stimulate nerve growth factor synthesis and secretion by astrocytes. (1991) Brain Research. 538(1):118-26.

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Gage, F.H., Fisher, L.J. Intracerebral grafting: a tool for the neurobiologist. (1991) Neurona. 6(1):1-12.

Hellweg, R., Fischer, W., Hock, C., Gage, F.H., Björklund, A., Thoenen, H. Nerve growth factor levels and choline acetyltransferase activity in the brain of aged rats with spatial memory impairments. (1990) Brain Research. 537(1-2):123-30.

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Tuszynski, M.H., U, H.S., Amaral, D.G., Gage, F.H. Nerve growth factor infusion in the primate brain reduces lesion-induced cholinergic neuronal degeneration. (1990) Diario de Neurociencia. 10(11):3604-14.

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Gage, F.H. Intracerebral grafting of genetically modified cells acting as biological pumps. (1990) Tendencias en Ciencias Farmacológicas. 11(11):437-9.

Qian, N., Sejnowski, T.J. When is an inhibitory synapse effective? (1990) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 87(20):8145-8149.

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Erselius, J.R., Goulding, M.D., Gruss, P. Structure and expression pattern of the murine Hox-3.2 gene. (1990) Development. 110(2):629-642.

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Fagan, A.M., Gage, F.H. Cholinergic sprouting in the hippocampus: a proposed role for IL-1. (1990) Exp. Neurol. 110(1):105-20.

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Jinnah, H.A., Gage, F.H., Friedmann, T. Animal models of Lesch-Nyhan syndrome. (1990) Brain Research Bulletin. 25(3):467-75.

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Foster, G.A., Brodin, E., Gage, F.H., Maxwell, D.J., Roberts, M.H., Sharp, T. Restoration of function to the denervated spinal cord after implantation of embryonic 5HT- and substance P-containing raphe neurones. (1990) Progress in Brain Research. 82:247-59.

Pfaff, S.L., Tamkun, M.M., Taylor, W.L. pOEV: a Xenopus oocyte protein expression vector. (1990) Analytical Biochemistry. 188(1):192-9.

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Lehky, S.R., Sejnowski, T.J. Neural model of stereoacuity and depth interpolation based on a distributed representation of stereo disparity. (1990) Diario de Neurociencia. 10(7):2281-2299.

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Hatzopoulos, A.K., Stoykova, A.S., Erselius, J.R., Goulding, M., Neuman, T., Gruss, P. Structure and expression of the mouse Oct2a and Oct2b, two differentially spliced products of the same gene. (1990) Development. 109(2):349-362.

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Lehky, S.R., Sejnowski, T.J. Neural network model of visual cortex for determining surface curvature from images of shaded surfaces. (1990) Proceedings of the Royal Society of London. Series B, Biological sciences. 240(1298):251-278.

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Lehky, S.R., Pouget, A., Sejnowski, T.J. Neural models of binocular depth perception. (1990) Cold Spring Harbor Symposia on Quantitative Biology. 55:765-777.

Shimohama, S., Saitoh, T., Gage, F.H. Differential expression of protein kinase C isozymes in rat cerebellum. (1990) J. Chem. Neuroanat. 3(5):367-75.

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Buzsáki, G., Chen, L.S., Gage, F.H. Spatial organization of physiological activity in the hippocampal region: relevance to memory formation. (1990) Progress in Brain Research. 83:257-68.

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Fisher, L.J., Young, S.J., Groves, P.M., Gage, F.H. Extracellular properties of cells within mesencephalon suspension grafts in rat striatum. (1990) Progress in Brain Research. 82:473-9.

Higgins, G.A., Koh, S., Neve, R.L., Mufson, E.J., Chen, K.S., Gage, F.H. Trophic regulation of basal forebrain gene expression in aging and Alzheimer's disease. (1990) Progress in Brain Research. 86:239-55.

Callaway, E.M., Katz, L.C. Emergence and refinement of clustered horizontal connections in cat striate cortex. (1990) Diario de Neurociencia. 10(4):1134-1153.

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Pyramidal cells in layer 2/3 of adult cat striate cortex have long, intrinsic horizontal axon collaterals within both layer 2/3 and layer 5. These collaterals form periodic "clusters" of finer axon branches that link columns of similar orientation selectivity. We have investigated the sequence of events and possible mechanisms underlying the development of these clustered intrinsic horizontal connections using a combination of neuronal tracers and intracellular staining. Small injections of fluorescent latex microspheres made during the first postnatal week (at P4-6), when examined in tangential sections, produced an even, unclustered distribution of retrogradely labeled cells up to 2 mm from the injection site. At P8, retrograde labeling extended over a larger area and clustering was discernible, primarily among the most distant labeled cells. At both P6 and P8, labeling was similar in layers 2/3 and 5, indicating that the transition from clustered to unclustered connections occurred simultaneously for cells in superficial and deep laminae. By the end of the second postnatal week (P12-15), retrogradely labeled cells were far more clustered both within and beyond the extent of P6 label; the density of labeled cells was high throughout the labeled region, but much higher within clusters. The periodicity of these nascent clusters was similar to that in the adult. Despite obvious clustering, the pattern of retrograde label observed following injections at 2-3 weeks (P12-21) differed markedly from the adult, in that the regions between clusters contained many labeled cells. Over the next 3 weeks, the connections were refined, so that by the sixth postnatal week (P36-38), regions between clusters contained very few retrogradely labeled cells and the overall pattern of retrograde label was indistinguishable from that in adults. Despite differences in postmigratory ages of neurons from the superficial and deep laminae, clustering of retrogradely labeled cells from these 2 populations was similar at all ages. Experiments in which 2-3 weeks elapsed between the time microsphere injections were made and animals were killed demonstrated that neither the initial formation of crude clusters nor their refinement was due to cell death. Instead, cluster refinement resulted from specific process elimination. When a red microsphere injection at P15 was followed by a green microsphere injection at exactly the same location on P29, the earlier injection resulted in crude clustering, as expected. Virtually all of the cells double-labeled by the later injection were within the densest clusters of label from the early injection.(ABSTRACT TRUNCATED AT 400 WORDS)

Thal, L.J., Mandel, R.J., Terry, R.D., Buzsaki, G., Gage, F.H. Nucleus basalis lesions fail to induce senile plaques in the rat. (1990) Experimental Neurology. 108(1):88-90.

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Higgins, G.A., Oyler, G.A., Neve, R.L., Chen, K.S., Gage, F.H. Altered levels of amyloid protein precursor transcripts in the basal forebrain of behaviorally impaired aged rats. (1990) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 87(8):3032-6.

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Tuszyński, M.H., Gage, F.H. Potential use of neurotrophic agents in the treatment of neurodegenerative disorders. (1990) Acta Neurobiologiae Experimentalis. 50(4-5):311-22.

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Dragunow, M., Goulding, M., Faull, R.L., Ralph, R., Mee, E., Frith, R. Induction of c-fos mRNA and protein in neurons and glia after traumatic brain injury: pharmacological characterization. (1990) Exp. Neurol. 107(3):236-248.

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Tuszynski, M.H., Buzsaki, G., Gage, F.H. Nerve growth factor infusions combined with fetal hippocampal grafts enhance reconstruction of the lesioned septohippocampal projection. (1990) Neuroscience. 36(1):33-44.

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Tuszynski, M.H., Armstrong, D.M., Gage, F.H. Basal forebrain cell loss following fimbria/fornix transection. (1990) Brain Research. 508(2):241-8.

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Buzsáki, G., Smith, A., Berger, S., Fisher, L.J., Gage, F.H. Petit mal epilepsy and parkinsonian tremor: hypothesis of a common pacemaker. (1990) Neuroscience. 36(1):1-14.

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Short, M.P., Rosenberg, M.B., Ezzedine, Z.D., Gage, F.H., Friedmann, T., Breakefield, X.O. Autocrine differentiation of PC12 cells mediated by retroviral vectors. (1990) Developmental Neuroscience. 12(1):34-45.

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Shults, C.W., Hashimoto, R., Brady, R.M., Gage, F.H. Dopaminergic cells align along radial glia in the developing mesencephalon of the rat. (1990) Neuroscience. 38(2):427-36.

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Mahanthappa, N.K., Gage, F.H., Patterson, P.H. Adrenal chromaffin cells as multipotential neurons for autografts. (1990) Progress in Brain Research. 82:33-9.

Gage, F.H., Buzsáki, G., Armstrong, D.M. NGF-dependent sprouting and regeneration in the hippocampus. (1990) Progress in Brain Research. 83:357-70.

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Gage, F.H., Rosenberg, M.B., Tuszynski, M.H., Yoshida, K., Armstrong, D.M., Hayes, R.C., Friedmann, T. Gene therapy in the CNS: intracerebral grafting of genetically modified cells. (1990) Progress in Brain Research. 86:205-17.

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Gage, F.H., Fisher, L.J., Jinnah, H.A., Rosenberg, M.B., Tuszynski, M.H., Friedmann, T. Grafting genetically modified cells to the brain: conceptual and technical issues. (1990) Progress in Brain Research. 82:1-10.

Goulding, M.D., Gruss, P. The homeobox in vertebrate development. (1989) Current Opinion in Cell Biology. 1(6):1088-93.

Dawson, V.L., Gage, F.H., Hunt, M.A., Wamsley, J.K. Normalization of subtype-specific muscarinic receptor binding in the denervated hippocampus by septodiagonal band grafts. (1989) Exp. Neurol. 106(2):115-24.

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Chen, K.S., Tuszynski, M.H., Gage, F.H. Role of neurotrophic factors in Alzheimer's disease. (1989) Neurobiology of Aging. 10(5):545-6; discussion 552-3.

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Chattarji, S., Stanton, P.K., Sejnowski, T.J. Commissural synapses, but not mossy fiber synapses, in hippocampal field CA3 exhibit associative long-term potentiation and depression. (1989) Brain Research. 495(1):145-50.

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Higgins, G.A., Koh, S., Chen, K.S., Gage, F.H. NGF induction of NGF receptor gene expression and cholinergic neuronal hypertrophy within the basal forebrain of the adult rat. (1989) Neurona. 3(2):247-56.

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Dragunow, M., Abraham, W.C., Goulding, M., Mason, S.E., Robertson, H.A., Faull, R.L. Long-term potentiation and the induction of c-fos mRNA and proteins in the dentate gyrus of unanesthetized rats. (1989) Neurosci. Lett. 101(3):274-80.

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Buzsáki, G., Bayardo, F., Miles, R., Wong, R.K., Gage, F.H. The grafted hippocampus: an epileptic focus. (1989) Exp. Neurol. 105(1):10-22.

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Field potentials and unitary activity were investigated in the grafted and the host hippocampi in freely moving rats and in vitro. The subcortical afferents and efferents of the hippocampus (fimbria-fornix, FF) were removed by aspiration. Solid pieces of hippocampal grafts derived from 15- to 16-day-old fetuses were placed in the lesion cavity in rats with unilateral FF lesions, and cell suspensions prepared from fetal hippocampi were grafted directly into the host hippocampi in animals with bilateral FF lesions. Reciprocal communication between the grafted and the host hippocampi was monitored with a 16-microelectrode probe from 7 to 10 months after grafting. The fluorescent retrograde tracer, Fluorogold, was used to examine graft-host projections and acetylcholinesterase staining to reveal host-derived fibers in the graft. The most typical neuronal pattern of the hippocampal graft was a highly synchronous population burst with concurrent EEG spike. The speed of propagation of the EEG spike within the graft and across the graft-host interface was either fast (greater than 3 m/s) or slow (less than 0.5 m/s). Large amplitude, short duration EEG spikes usually propagated with a high speed, while smaller amplitude, wider spikes with broad population bursts spread at a lower velocity. The direction of propagation was usually uniform indicating that the population burst was triggered by a localized subgroup of highly excitable neurons ("focus"). Spontaneous seizures were also present in the solid graft which frequently invaded the host hippocampus. The incidence of EEG spikes was three times higher in rats with bilateral suspension grafts than in animals with FF lesion only. In about half of the grafted rats spontaneous behavioral seizures were also observed. Intracellular recordings from putative pyramidal cells in the graft and in the host revealed large amplitude (10-12 mV), spontaneously occurring EPSPs. IPSPs were difficult to detect even during depolarizations of up to 20 mV from rest. We suggest that the increased excitability of the hippocampal graft is due to the high incidence of recurrent excitatory collaterals terminating on or close to the somata of pyramidal neurons. Population bursts may spread fast via extensively arborizing axon collaterals or slowly by successively activating new sets of neighboring neurons. Spontaneous behavioral convulsions are explained by assuming that the grafted hippocampus serves as an epileptic focus which is capable of kindling the host brain by repeated seizure induction.(ABSTRACT TRUNCATED AT 400 WORDS)

Batchelor, P.E., Armstrong, D.M., Blaker, S.N., Gage, F.H. Nerve growth factor receptor and choline acetyltransferase colocalization in neurons within the rat forebrain: response to fimbria-fornix transection. (1989) Journal of Comparative Neurology. 284(2):187-204. DOI: 10.1002/cne.902840204

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Although it is well known that magnocellular cholinergic basal forebrain neurons are trophically responsive to nerve growth factor (NGF) and contain NGF receptors (NGFr), the exact distribution of forebrain NGFr-immunoreactive neurons and the degree to which cholinergic neurons are colocalized with them have remained in question. In this study we employed a very sensitive double-labelling method and examined in the same tissue section the distribution and cellular features of NGFr-positive and choline acetyltransferase (ChAT)-immunolabelled neurons within the rat basal forebrain. Throughout this region the majority of magnocellular basal forebrain neurons were immunoreactive for both NGFr and ChAT. However, a small percentage of neurons in the ventral portion of the vertical limb of the diagonal band of Broca were immunoreactive only for NGFr, whereas a larger population of magnocellular neurons in the substantia innominata exhibited only ChAT immunoreactivity. No NGFr-immunoreactive cells were found associated with ChAT-positive neurons in the striatum, neocortex, or hippocampus, and no single-labelled NGFr-immunoreactive neurons were found outside the basal forebrain area, except for a large number of positive-labelled cells along the ventricular walls of the third ventricle. In addition to its function in maintaining the normal integrity of the basal forebrain and cholinergic, peripheral sympathetic, and neural-crest-derived sensory neurons, NGF may also have a role in the growth of these neurons after damage to the nervous system. To examine this postulate the hippocampus was denervated of its septal input and examined 8 weeks later. Two populations of neurons were found to have undergone collateral sprouting--namely, the midline magnocellular cholinergic neurons of the dorsal hippocampus and the sympathetic noradrenergic neurons of the superior cervical ganglion. Both of these neuronal populations also stained strongly for NGFr. In contrast, the small intrinsic cholinergic neurons of the hippocampus exhibited neither sprouting response nor staining for NGFr. In view of these results, we suggest that the differing sprouting responses demonstrated by these three neuronal populations may be due to their responsiveness to NGF, as indicated by the presence or absence of NGF receptors.

Buzsàki, G., Bickford, R.G., Ryan, L.J., Young, S., Prohaska, O., Mandel, R.J., Gage, F.H. Multisite recording of brain field potentials and unit activity in freely moving rats. (1989) Journal of Neuroscience Methods. 28(3):209-17.

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Mandel, R.J., Gage, F.H., Thal, L.J. Spatial learning in rats: correlation with cortical choline acetyltransferase and improvement with NGF following NBM damage. (1989) Exp. Neurol. 104(3):208-17.

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Shimohama, S., Rosenberg, M.B., Fagan, A.M., Wolff, J.A., Short, M.P., Breakefield, X.O., Friedmann, T., Gage, F.H. Grafting genetically modified cells into the rat brain: characteristics of E. coli beta-galactosidase as a reporter gene. (1989) Brain Res. Mol. Brain Res. 5(4):271-8.

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Buzsáki, G., Ponomareff, G.L., Bayardo, F., Ruiz, R., Gage, F.H. Neuronal activity in the subcortically denervated hippocampus: a chronic model for epilepsy. (1989) Neuroscience. 28(3):527-38.

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Spontaneous and evoked field potentials and cellular discharges were studied in the subcortically denervated hippocampus of the freely moving rat. The fimbria fornix, the ventral hippocampal commissure, and the supracallosal afferent fibers were removed by aspiration, and recordings were made 3-5 months after the lesion. Two types of spontaneous interictal spikes were observed. Type 1 interictal spike had identical depth distribution to physiological sharp waves but they were shorter in duration (less than 40 ms), larger in amplitude (greater than 2.5 mV) and population spikes were riding on the main deflection. Type 2 interictal spikes were negative in the stratum oriens and positive in the pyramidal layer and stratum radiatum of both CA1 and CA3. The amplitude of both types of interictal spikes could exceed 6 mV. We suggest that interictal spikes were initiated randomly in different subpopulations of the CA2-3 region and the location of the initiating population burst determined the polarity and amplitude of the extracellular interictal spike. Repetitive stimulation of the perforant path (5 Hz, 6 s) evoked markedly uniform afterdischarges in both intact and fimbria fornix-deprived rats. The threshold of afterdischarges was significantly lower, the seizure spread to the contralateral hippocampus was slower, and secondary afterdischarges lasted significantly longer in the lesioned rats. We suggest that under physiological conditions the electrical stability of the hippocampus is ensured by the feed-forward inhibitory action of subcortical afferents. Removal of tonic inhibitory influences and/or sprouting of local axon collaterals allows extreme synchronization and reverberation of information in the entorhinal-hippocampal-entorhinal cortex circuitry. The presence of interictal spikes and increased susceptibility to seizures for several months after the lesion offers the fimbria-fornix-deprived hippocampus a useful chronic preparation to study the mechanisms of limbic epilepsy.

Shapiro, M.L., Simon, D.K., Olton, D.S., Gage, F.H., Nilsson, O., Björklund, A. Intrahippocampal grafts of fetal basal forebrain tissue alter place fields in the hippocampus of rats with fimbria-fornix lesions. (1989) Neuroscience. 32(1):1-18.

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Gage, F.H., Tuszynski, M.H., Chen, K.S., Armstrong, D., Buzsáki, G. Survival, growth and function of damaged cholinergic neurons. (1989) EXS. 57:259-74.

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Buzsàki, G., Gage, F.H. The cholinergic nucleus basalis: a key structure in neocortical arousal. (1989) EXS. 57:159-71.

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Callaway, E.M., Soha, J.M., Van Essen, D.C. Differential loss of neuromuscular connections according to activity level and spinal position of neonatal rabbit soleus motor neurons. (1989) Diario de Neurociencia. 9(5):1806-1824.

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We have tested whether the ability of synapses to compete for occupancy of endplates during neuromuscular synapse elimination is affected by differences in the spinal position or in the activity level of the parent motor neuron. To test the role of spinal position, the relative sizes of motor units for motor neurons from middle and extreme (rostral/caudal) positions in the rabbit soleus motor pool were determined at 3 postnatal ages: 4-5 d ("early" ages, when the soleus is heavily polyinnervated), 8-9 d ("intermediate"), and 11-15 d ("late," when the soleus has just reached singly innervated state). Average motor unit sizes from extreme ventral roots were similar to those from middle ventral roots in early-aged soleus muscles but were significantly smaller (by 18-27%) for both intermediate and late muscles. Thus, motor neurons from extreme positions evidently compete less effectively for retention of synapses than those from middle positions. To test the role of differential activity, inactive and active synapses were pitted directly against one another by implanting Silastic plugs laden with tetrodotoxin (TTX) into one of the spinal nerves containing a minority of the soleus motor axons. Differential activity was maintained during a period of extensive synapse loss, from the time of the implant at day 4 or 5 until the intermediate age (day 8-9). Motor unit twitch tensions were subsequently measured to determine the relative number of synapses retained by individual active and inactive motor neurons. The inactivated motor units were on average significantly larger (by more than 50%) than the corresponding group from normal and control-implanted animals. The abnormally large size of inactivated motor units persisted in animals allowed to recover from the TTX block and examined after multiple innervation had disappeared. Hence, the effect of the TTX block cannot be attributed to a simple slowing of synapse elimination specifically among the inactive motor neurons. We conclude that complete presynaptic inactivity improves the chances of survival relative to that for normal activity during synapse elimination in the neonatal rabbit soleus muscle. This difference in competitive ability may contribute to the development of an important characteristic of adult muscles, the correlation between motor unit size and recruitment threshold.

Stanton, P.K., Sejnowski, T.J. Associative long-term depression in the hippocampus induced by hebbian covariance. (1989) Naturaleza. 339(6221):215-8. DOI: 10.1038/339215a0

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Tuszynski, M.H., Jinnah, H.A., Gage, F.H. Delivery of neuroactive compounds to the brain: potential utility of genetically modified cells. (1989) Neurobiology of Aging. 10(5):644-5; discussion 648-50.

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Gage, F.H., Dunnett, S.B., Bjorklund, A. Age-related impairments in spatial memory are independent of those in sensorimotor skills. (1989) Neurobiology of Aging. 10(4):347-52.

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Phelps, C.H., Gage, F.H., Growdon, J.H., Hefti, F., Harbaugh, R., Johnston, M.V., Khachaturian, Z.S., Mobley, W.C., Price, D.L., Raskind, M. Potential use of nerve growth factor to treat Alzheimer's disease. (1989) Neurobiology of Aging. 10(2):205-7.

Buzsáki, G., Wiesner, J., Henriksen, S.J., Gage, F.H. Long-term potentiation of evoked and spontaneous neuronal activity in the grafted hippocampus. (1989) Exp Brain Res. 76(2):401-8.

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Buzsáki, G., Gage, F.H. Absence of long-term potentiation in the subcortically deafferented dentate gyrus. (1989) Brain Research. 484(1-2):94-101.

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Callaway, E.M., Van Essen, D.C. Slowing of synapse elimination by alpha-bungarotoxin superfusion of the neonatal rabbit soleus muscle. (1989) Developmental Biology. 131(2):356-365.

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Soha, J.M., Callaway, E.M., Van Essen, D.C. Lack of fiber type selectivity during reinnervation of neonatal rabbit soleus muscle. (1989) Developmental Biology. 131(2):401-414.

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Gage, F.H., Batchelor, P., Chen, K.S., Chin, D., Higgins, G.A., Koh, S., Deputy, S., Rosenberg, M.B., Fischer, W., Bjorklund, A. NGF receptor reexpression and NGF-mediated cholinergic neuronal hypertrophy in the damaged adult neostriatum. (1989) Neurona. 2(2):1177-84.

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Fischer, W., Gage, F.H., Björklund, A. Degenerative Changes in Forebrain Cholinergic Nuclei Correlate with Cognitive Impairments in Aged Rats. (1989) European Journal of Neuroscience. 1(1):34-45.

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Goulding, M.D., Ralph, R.K. Cyclic-AMP-induced c-fos expression and its relevance to differentiation of a transformed mast cell line. (1989) Bioquímica y Biofísica Acta. 1007(1):99-108.

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Mandel, R.J., Gage, F.H., Thal, L.J. Enhanced detection of nucleus basalis magnocellularis lesion-induced spatial learning deficit in rats by modification of training regimen. (1989) Behavioural Brain Research. 31(3):221-9.

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Foster, G.A., Roberts, M.H., Wilkinson, L.S., Björklund, A., Gage, F.H., Hökfelt, T., Schultzberg, M., Sharp, T. Structural and functional analysis of raphe neurone implants into denervated rat spinal cord. (1989) Brain Research Bulletin. 22(1):131-7.

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Rosenberg, M.B., Friedmann, T., Robertson, R.C., Tuszynski, M., Wolff, J.A., Breakefield, X.O., Gage, F.H. Grafting genetically modified cells to the damaged brain: restorative effects of NGF expression. (1988) Ciencia. 242(4885):1575-8.

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Buzsáki, G., Ponomareff, G., Bayardo, F., Shaw, T., Gage, F.H. Suppression and induction of epileptic activity by neuronal grafts. (1988) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 85(23):9327-30.

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Churchland, P.S., Sejnowski, T.J. Perspectives on cognitive neuroscience. (1988) Ciencia. 242(4879):741-5.

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Buzsaki, G., Bickford, R.G., Ponomareff, G., Thal, L.J., Mandel, R., Gage, F.H. Nucleus basalis and thalamic control of neocortical activity in the freely moving rat. (1988) Diario de Neurociencia. 8(11):4007-26.

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EEG and single-unit techniques have been used to study the EEG correlates of cellular firing in the neocortex, n. reticularis (RT) and "specific" thalamic nuclei, and the cholinergic forebrain area (nucleus basalis, NB). Neuronal firing was related to the ongoing behavior of the rat. In addition, using a 16-channel neocortical recording/mapping system, we studied the effects of ibotenic acid lesion of NB, RT, and other thalamic nuclei on the patterns and spatial distribution of neocortical electrical activity. The majority of neurons in neocortex, NB, and RT increased their firing rates during walking, as compared to during immobility, with concurrent decrease of delta power in the neocortical EEG. During immobility, high-voltage spindles (HVS; greater than 1 mV) were occasionally recorded from the neocortex. Depth profiles of HVS and slow delta waves were different in the neocortex. Neocortical cells decreased their discharge frequency during the positive portion of delta waves recorded in layers V and VI. All cells in the neocortex and specific thalamic nuclei fired rhythmically and phase-locked to the spike component of HVS. RT neurons showed an opposite phase relationship and fired mainly during the wave component of HVS. Half of the NB neurons also showed phasic modulation with HVS. Circumscribed lesion of RT and extensive damage of other thalamic regions, including the intralaminar nuclei, suppressed HVS but had no effect on the neocortical EEG correlates of behavior. In sharp contrast, damage to the NB resulted in a dramatic increase of slow delta waves on the side of the lesion, mimicking the effect of scopolamine administration. We suggest that the NB plays a key role in neocortical arousal by directly activating the neocortex and by suppressing the rhythm generation in the RT-thalamocortical circuitry. We further suggest that the NB system may serve as a structural basis for the concept of the generalized ascending activation of Moruzzi and Magoun (1949).

Pfaff, S.L., Duesberg, P.H. Two autonomous myc oncogenes in avian carcinoma virus OK10. (1988) Journal of Virology. 62(10):3703-9.

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Gage, F.H., Olejniczak, P., Armstrong, D.M. Astrocytes are important for sprouting in the septohippocampal circuit. (1988) Exp. Neurol. 102(1):2-13.

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Sejnowski, T.J., Koch, C., Churchland, P.S. Computational neuroscience. (1988) Ciencia. 241(4871):1299-306.

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Buzsáki, G., Bickford, R.G., Armstrong, D.M., Ponomareff, G., Chen, K.S., Ruiz, R., Thal, L.J., Gage, F.H. Electric activity in the neocortex of freely moving young and aged rats. (1988) Neuroscience. 26(3):735-44.

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Brundin, P., Strecker, R.E., Gage, F.H., Lindvall, O., Björklund, A. Intracerebral transplantation of dopamine neurons: understanding the functional role of the mesolimbocortical dopamine system and developing a therapy for Parkinson's disease. (1988) Annals of the New York Academy of Sciences. 537:148-60.

Qian, N., Sejnowski, T.J. Predicting the secondary structure of globular proteins using neural network models. (1988) Revista de Biología Molecular. 202(4):865-84.

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Armstrong, D.M., Hersh, L.B., Gage, F.H. Morphologic alterations of cholinergic processes in the neocortex of aged rats. (1988) Neurobiology of Aging. 9(2):199-205.

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Lehky, S.R., Sejnowski, T.J. Network model of shape-from-shading: neural function arises from both receptive and projective fields. (1988) Naturaleza. 333(6172):452-4. DOI: 10.1038/333452a0

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Gage, F.H., Chen, K.S., Buzsaki, G., Armstrong, D. Experimental approaches to age-related cognitive impairments. (1988) Neurobiology of Aging. 9(5-6):645-55.

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Rats exhibit morphological, biochemical, and metabolic changes in their brains, as well as cognitive deficits, with aging. Aged rats were found to be significantly impaired compared to young rats in a water maze task and test of motor coordination, and show reduced locomotor activity and exploration. Although aged rats did exhibit deficits as a group, not all aged rats were impaired. Additionally, the subgroup that was impaired on one task was not necessarily the subgroup that was impaired on another task. The cholinergic projection neurons in the basal forebrain region were significantly atrophied in the aged rodent. The degree of atrophy was highly correlated with the cognitive impairment exhibited on the Morris water maze task. Swollen choline acetyltransferase (ChAT)-positive "plaque-like" structures were observed in the neocortex of the aged but not the young rats. Declines in cholinergic activity in the brain has also been observed during aging. Biochemical measurements of ChAT in the basal forebrain region of aged rats revealed small but consistent decreases in ChAT activity compared to young rats. General metabolic activity, measured by the 2-deoxyglucose method, was also decreased in the hippocampal CA1 and CA3 fields, the dentate gyrus, the medial septal-diagonal band area, and the prefrontal cortex of aged rats. There was a significant correlation between the decrease in glucose utilization and deficits on the Morris water maze. Most aged rats exhibit pathological EEG patterns as reflected by frequent long-duration high voltage neocortical spindles (HVS) during immobility. Bilateral lesions of the nucleus basalis and scopolamine treatment increased the incidence of HVS, thereby mimicking changes in the aged brain. We attempted to ameliorate the cognitive deficits observed in subgroups or impaired rats by either: (1) implanting fetal cells of basal forebrain origin into the hippocampus, or (2) infusing nerve growth factor (NGF) chronically into the lateral ventricle. The grafts appeared to facilitate an improvement in the ability of the impaired aged rats to perform in the Morris water maze. This improved performance was reversed by injections of atropine at doses that did not affect the behavior of young animals that performed well in the same task. These results suggest that enhancement of the cholinergic system could have an effect on the performance of the impaired aged animals. The study of the effects of infusions of NGF clearly demonstrate that the ability of impaired aged rats to remember what they had previously learned was increased after NGF treatment.(ABSTRACT TRUNCATED AT 400 WORDS)

Blaker, S.N., Armstrong, D.M., Gage, F.H. Cholinergic neurons within the rat hippocampus: response to fimbria-fornix transection. (1988) Journal of Comparative Neurology. 272(1):127-38. DOI: 10.1002/cne.902720109

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The distribution and morphologic characteristics of choline acetyltransferase (ChAT)-containing neurons were studied throughout the rostrocaudal extent of the rat hippocampus and in a midline area just dorsal to the dorsal hippocampus. Peroxidase reaction product was observed with the aid of immunohistochemical methods and a high-titer polyclonal antibody against ChAT, the acetylcholine biosynthetic enzyme. ChAT-positive cells in the hippocampus were characterized by small, round or oval perikarya with two or more proximal processes. They were located within the caudal and temporal hippocampal formation, predominantly within the subiculum, in the stratum lacunosum moleculare, at the border of the stratum lacunosum moleculare and the stratum radiatum, and in the molecular layer of the dentate gyrus. The cells resembled in morphology the small, bipolar and multipolar neocortical ChAT-immunoreactive cells. In addition to the hippocampus, ChAT-positive neurons were observed caudally in a region just above the dorsal hippocampal commissure and rostrally in the columns of the fornix. These cells were large with an oval perikarya and darkly labeled compared to neurons in the hippocampus. They more closely resembled the ChAT-positive neurons in the midline raphe of the medial septal nucleus. Examination of the rat hippocampus 2 and 8 weeks following unilateral lesioning of the fimbria-fornix and supracallosal striae revealed a sparse innervation of ChAT-positive fibers in the hippocampus ipsilateral to the lesion. ChAT-labeled neurons in the hippocampus did not appear to sprout in response to the lesion. In contrast, ChAT-positive cells in the midline did appear to sprout into the medial dorsal subiculum and dorsal medial hippocampus. We conclude that these two populations of cells are distinct with respect to their response to hippocampal denervation and, furthermore, that this distinction may be attributed to a differential response to nerve growth factor.

Gage, F.H., Blaker, S.N., Davis, G.E., Engvall, E., Varon, S., Manthorpe, M. Human amnion membrane matrix as a substratum for axonal regeneration in the central nervous system. (1988) Experimental Brain Research. 72(2):371-80.

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Human Amnion Membrane Matrix (HAMM) was used as a substratum for the regeneration of neuronal axons in the central nervous system. A large piece of HAMM was bound to nitrocellulose paper (NCP) as a supporting material, and cut into small strips. Aspirative lesions of the fimbria-fornix were made in adult rats leaving a cavity separating the septum from the hippocampus. In the same operation a small piece of HAMM-NCP was placed into the cavity with one end abutting the axotomized septum and the other end abutting the denervated hippocampus. At times between 2 weeks and 3 months after surgery the brains of the animals were examined histologically for 1) host response to the implant; 2) maintenance of HAMM-NCP in the originally implanted orientation; 3) growth of acetylcholinesterase (AChE)-positive fibers on the HAMM; 4) growth of the AChE-positive fibers into the denervated hippocampus; and 5) non-neuronal cells on the HAMM. The NCP remained in place over the 3-month period. In most cases the HAMM and NCP remained apposed and caused no greater reaction in the brain beyond that created in response to the aspirative lesion alone. AChE-positive fibers grew out from the septum onto the HAMM by 2 weeks, and by 8 weeks more extensive growth was observed on the HAMM. By 8-weeks, AChE fibers could be clearly seen coming off the membrane and entering the host hippocampus. By double-labelling for the basement membrane side of the HAMM (using anti-human laminin antibodies) and for cholinergic fibers (using AChE histochemistry) in the same sections, it was possible to see fibers clearly growing on the laminin-positive side of the HAMM but little or no growth on the opposing laminin-negative stromal side. The most important variables for extensive growth appeared to be the accurate placement of the implant and the amount of time following the lesions. The placement of the matrix on NCP prior to implantation in the brain made it easier to orient the membrane between the septum and the hippocampus, and seemed to assist in maintaining the membrane in that orientation. The HAMM represents a useful, regeneration-promoting grafting material for central nervous system repair studies.

Buzsáki, G., Freund, T., Björklund, A., Gage, F.H. Restoration and deterioration of function by brain grafts in the septohippocampal system. (1988) Progress in Brain Research. 78:69-77.

Blaker, S.N., Davis, G.E., Manthorpe, M., Engvall, E., Varon, S., Gage, F.H. Human amnion membrane as a substratum for axonal elongation in vitro and in vivo. (1988) Progress in Brain Research. 78:435-8.

Foster, G.A., Schultzberg, M., Gage, F.H., Björklund, A., Hökfelt, T., Cuello, A.C., Verhofstad, A.A., Visser, T.J., Emson, P.C. Transmitter expression and morphological development of embryonic medullary and mesencephalic raphé neurones after transplantation to the adult rat central nervous system. III. Grafts to the striatum. (1988) Exp Brain Res. 70(2):242-55.

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Foster, G.A., Schultzberg, M., Gage, F.H., Björklund, A., Hökfelt, T., Cuello, A.C., Verhofstad, A.A., Visser, T.J., Emson, P.C. Transmitter expression and morphological development of embryonic medullary and mesencephalic raphé neurones after transplantation to the adult rat central nervous system. II. Grafts to the hippocampu (1988) Experimental Brain Research. 70(2):225-41.

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Suspensions of cells taken from the mesencephalic or medullary raphé regions of the 13-14 day old embryonic rat brain were injected into the hippocampus of adult rats which had previously been denervated of its serotoninergic input by 5,7-dihydroxytryptamine. At periods of up to 14 months after implantation, the brains were taken for immunohistochemical analysis of 5-hydroxytryptamine (5HT)-, substance P (SP)- and thyrotropin releasing hormone (TRH)-like immunoreactivity. Surviving grafts were found in all animals. The implants derived from mesencephalic raphé contained neurones immunoreactive to 5HT, SP or both substances together. On average 19% of the potential number of mesencephalic 5HT neurones were found in the grafts. Outgrowth of 5HT-immunoreactive fibres was extensive, and displayed the typical pattern of 5HT innervation in the normal hippocampus-the densest plexuses were found in the dentate gyrus, with sparser networks in the CA1 and CA2 regions. SP-positive processes were principally found only in the graft itself. Transplants of medullary raphé cells contained 5HT-immunoreactive neurones, some of which also contained SP-and/or TRH-like immunoreactivity, thereby mirroring the situation found in the caudal raphé complex in situ. An average of 18% of the total available medullary serotoninergic neurones were found at each graft site. A rich outgrowth of 5HT-immunoreactive varicose processes was evident, with the same pattern as the 5HT innervation by the mesencephalic raphé grafts, and as in the normal hippocampus. SP- and TRH-positive fibres were essentially detectable only in the graft, but not in the host hippocampus. The present studies indicate that the milieu of the hippocampus does not preferentially attenuate the survival of the serotoninergic cells which do not normally project to it. Nor is the fibre outgrowth of these medullary raphé 5HT neurones significantly different from that of the mesencephalic raphé. However, the hippocampal environment may be responsible for the appearance of SP-LI in the otherwise apparently solely 5HT-containing mesencephalic raphé neurones, and for repressing the outgrowth of fibres containing TRH-, or SP-like immunoreactivity, regardless of their origin.

Buzsáki, G., Gage, F.H. Mechanisms of action of neural grafts in the limbic system. (1988) Can J Neurol Sci. 15(2):99-105.

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Gage, F.H., Wolff, J.A., Rosenberg, M.B., Xu, L., Yee, J.K., Shults, C., Friedmann, T. Implantation of genetically engineered cells to the brain. (1988) Progress in Brain Research. 78:651-8.

Bengtsson, F., Nobin, A., Falck, B., Gage, F.H., Jeppsson, B. Effect of oral branched chain amino acids on behavior and brain serotonin metabolism in portacaval shunted rats. (1988) World Journal of Surgery. 12(2):246-54.

Sejnowski, T.J. Neural populations revealed. (1988) Naturaleza. 332(6162):308. DOI: 10.1038/332308a0

Gage, F.H., Armstrong, D.M., Williams, L.R., Varon, S. Morphological response of axotomized septal neurons to nerve growth factor. (1988) Journal of Comparative Neurology. 269(1):147-55. DOI: 10.1002/cne.902690112

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Gage, F.H., Brundin, P., Strecker, R., Dunnett, S.B., Isacson, O., Bjorklund, A. Intracerebral neuronal grafting in experimental animal models of age-related motor dysfunction. (1988) Annals of the New York Academy of Sciences. 515:383-94.

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The combined morphological, biochemical, electrophysiological, and behavioral data summarized above show that implanted embryonic nerve cells in some cases can substitute quite well for a lost intrinsic neuronal system in mammals. The intracerebral implants probably exert their effects in several ways. The functional effects seen with grafts placed into one of the cerebral ventricles (such as those described in the studies of Perlow et al., Freed et al., and Gash et al.) are thus probably explained on the basis of a diffuse release of an active amine or peptide into the host CSF and adjacent brain tissue. In other instances, as in animals with DA-rich grafts reinnervating the neostriatum, we believe that the available data provide quite substantial evidence that the behavioral recovery is caused by the ability of the grafted neurons to reinnervate relevant parts of the host brain. This is illustrated by the studies mentioned above that show that the degree of functional recovery in 6-OHDA-lesioned rats with nigral transplants is directly correlated with the extent of striatal DA reinnervation and that the "profile" of functional recovery is dependent on that area of the striatal complex that is reinnervated by the graft. This point is particularly well illustrated in a further study in which rats with electrodes implanted into the center of intracortical nigral grafts were allowed to "self-stimulate" via the graft. The results show that the graft can indeed sustain self-stimulation behavior and that the rate of lever-pressing is related to the proximity between the electrode tip and the DA-containing neurons in the graft. This strongly supports the notion that the implanted DA neurons can transmit behaviorally meaningful and temporally organized information to the host brain via their efferent connections. To what extent the intracerebral implants can be functionally integrated with the host brain is still poorly known, though, and it therefore remains an interesting question for further investigation. The chances for extensive integration may be greatest for neuronal suspension grafts implanted as deposits directly into the depth of the brain, but even solid grafts inserted as whole pieces into the brain have, in several cases, been seen to become reinnervated from the host brain in adult and developing recipients. Nevertheless, a recent HRP study failed to detect any host afferents to intracortical solid nigral grafts, despite the fact that these grafts had themselves formed extensive DA connections in the host striatum and had produced behavioral recovery.(ABSTRACT TRUNCATED AT 400 WORDS)

Armstrong, D.M., Bruce, G., Hersh, L.B., Gage, F.H. Development of cholinergic neurons in the septal/diagonal band complex of the rat. (1987) Brain Research. 433(2):249-56.

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Gage, F.H., Wolff, J.A., Rosenberg, M.B., Xu, L., Yee, J.K., Shults, C., Friedmann, T. Grafting genetically modified cells to the brain: possibilities for the future. (1987) Neuroscience. 23(3):795-807.

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Shults, C.W., Johnston, P., Gage, F.H. Comparison of substance K-like and substance P-like fibers and cells in the rat hippocampus. (1987) Brain Research. 426(2):290-6.

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Isacson, O., Pritzel, M., Dawbarn, D., Brundin, P., Kelly, P.A., Wiklund, L., Emson, P.C., Gage, F.H., Dunnett, S.B., Björklund, A. Striatal neural transplants in the ibotenic acid-lesioned rat neostriatum. Cellular and functional aspects. (1987) Annals of the New York Academy of Sciences. 495:537-55.

Armstrong, D.M., Terry, R.D., Deteresa, R.M., Bruce, G., Hersh, L.B., Gage, F.H. Response of septal cholinergic neurons to axotomy. (1987) Journal of Comparative Neurology. 264(3):421-36. DOI: 10.1002/cne.902640309

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In the present study we employed quantitative morphometric techniques to assay the response of septal cholinergic neurons following unilateral transection of the fimbria/fornix and supracallosal stria. Analysis of 50-micron-thick tissue sections with a Quantimet 920 image analysis system demonstrated a reduction in ChAT immunoreactivity as early as 1 day following denervation. This decrease was associated with a drop in the number of labeled cells ipsilateral to the lesion and a decrease in the area of cholinergic perikarya on the lesioned and nonlesioned side of the septum. The response at 1 day, however, was transient, and at 4 days the number of labeled neurons was not significantly different from controls. By 8 days we observed a dramatic reduction in the number and size of ChAT-positive cells ipsilateral to the lesion and a reduction in the size of cholinergic perikarya on the contralateral (i.e., nonlesioned) side. These values persisted throughout the remainder of the study. To assess more completely the morphologic response of neurons to axotomy than can be determined in 50-micron-thick tissue sections, we embedded the adjacent immunolabeled tissue section in Epon and then serially sectioned it to a thickness of 0.75-1.0 micron. By using this method, we were able to measure the area, length, and width of the cell, the area of the nucleus and nucleolus, and the position of the nucleus (i.e., eccentricity). Measurements were performed on ChAT-labeled and nonlabeled cells. The results of our studies demonstrate that cholinergic and noncholinergic cells responded to axotomy in a characteristic yet different fashion from each other and that this response could be quantitatively assayed. In general, labeled and nonlabeled cells on the lesioned side of the septum shrink in response to denervation. This shrunken state was reflected in measurements of cellular area, length, width, and nuclear area. Moreover, other measurements of cellular morphology (i.e., area of the nucleolus, position of the nucleus) indicate that none of the neuronal populations examined in the present study displayed morphologic evidence of regeneration. Our results indicate a dramatic loss of cholinergic perikarya ipsilateral to the lesion. Moreover, although a few neurons do persist they do so in a shrunken state. These data provide an essential baseline for the second study in this series, which will evaluate the effect of nerve growth factor on the survival of denervated septal neurons.

Buzsàki, G., Czopf, J., Kondàkor, I., Björklund, A., Gage, F.H. Cellular activity of intracerebrally transplanted fetal hippocampus during behavior. (1987) Neuroscience. 22(3):871-83.

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Hippocampal tissue derived from 12-, 20-, 25- and 34-mm rat fetuses was placed in a cavity formed by unilateral aspiration of the fimbria-fornix and the overlying neocortical tissue in adult rats. From 4 to 6 months after transplantation the rats were equipped with chronic recording and stimulating electrodes. Single cell activity of the transplant was monitored during running in a wheel, drinking, and sleeping. Both complex-spike cells (n = 151) and single-spike cells (n = 80) were recorded from the graft. A portion of the neurons changed their firing rates and discharge patterns as a function of ongoing behavior. About half of the single-spike cells increased their firing frequency during running. Fifteen per cent of the single-spike cells fired rhythmically at about 8 Hz during running, and the paradoxical phase of sleep and the discharge pattern correlated with rhythmic slow activity (theta) recorded concurrently from the contralateral (intact) hippocampus. These patterns were most frequently obtained from grafts of 20- and 25-mm (16 to 18 embryonic days) fetuses. Graft neurons could be activated by stimulating the ipsilateral hippocampus or the ipsilateral perforant path, with latencies of 8-30 ms. The most common electrical pattern in grafts of all groups was the synchronous bursts of several neighboring cells and concurrent electroencephalogram sharp-waves. Sharp-waves occurred during all behaviors. Large amplitude, high-frequency electroencephalogram spindles (14-18 Hz and 30-50 Hz) and associated neuronal bursts were recorded in grafts of 12-, 20-, and 25-mm fetuses. Based on these findings we suggest that both subcortical afferents and host hippocampal afferents send axons to hippocampal grafts and form viable synaptic connections with a portion of the neurons in the graft. The frequently encountered population bursts are explained by assuming that excitatory collaterals in the graft are more potent in the graft than in the normal hippocampus, and/or GABAergic inhibition is less efficient in the graft.

Isacson, O., Dawbarn, D., Brundin, P., Gage, F.H., Emson, P.C., Björklund, A. Neural grafting in a rat model of Huntington's disease: striosomal-like organization of striatal grafts as revealed by acetylcholinesterase histochemistry, immunocytochemistry and receptor autoradiogr (1987) Neuroscience. 22(2):481-97.

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Grafts of fetal striatum were implanted in the form of a cell suspension into the brains of rats with prior ibotenic acid lesions of the caudate-putamen. The grafts were placed in three different sites: the lesioned caudate-putamen, or the denervated (but otherwise undamaged) globus pallidus and substantia nigra. After 3-6 months survival the grafts were investigated by means of immunohistochemistry and receptor autoradiography in combination with routine histology and acetylcholinesterase histochemistry. The grafts placed within the lesioned caudate-putamen were at least 10-fold larger larger than those placed in the substantia nigra region, with the grafts placed in the globus pallidus being of intermediate size. In all locations the acetylcholinesterase staining had an uneven, patchy distribution, which was most pronounced in the grafts located within the caudate-putamen. These patches did not bear any obvious relationship to variations in density of the neuronal perikarya within the grafted tissue. Many of the neuropeptide-immunoreactive neuron types present in the normal striatum, such as those containing substance P, [Met]enkephalin, somatostatin, cholecystokinin and neuropeptide Y were also detected in the grafted striatum along with acetylcholinesterase-positive staining. Acetylcholinesterase-positive, [Met]enkephalin-positive, substance P-positive and tyrosine hydroxylase-positive markers all showed uneven, patchy distributions in the grafts. This was also the case for the distribution of dopamine D2 and opiate receptors (as revealed by [3H]spiroperidol and [3H]diprenorphine autoradiography, respectively), whereas muscarinic receptor binding was even throughout the grafts. As is the case in the so-called striosomal patches (neurochemically defined compartments) in the immature intact striatum during the early postnatal period, patches of high acetylcholinesterase staining in the grafts showed partial correspondence with patches of high [Met]enkephalin fibre staining, and dopamine receptor density, and (although to a lesser degree) also with patches of high opiate receptor density and high substance P-immunoreactivity. This correspondence of patches also occurred between tyrosine hydroxylase fibre staining and acetylcholinesterase staining as revealed by grafts placed into the substantia nigra. These results suggest that the fetal striatal cell suspension grafts will give rise to a fairly normal range of striatal neuron and receptor types and that they develop at least some of the striosomal features characteristic for the normal striatum.(ABSTRACT TRUNCATED AT 400 WORDS)

Kesslak, J.P., Frederickson, C.J., Gage, F.H. Quantification of hippocampal noradrenaline and zinc changes after selective cell destruction. (1987) Exp Brain Res. 67(1):77-84.

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Callaway, E.M., Soha, J.M., Van Essen, D.C. Competition favouring inactive over active motor neurons during synapse elimination. (1987) Naturaleza. 328(6129):422-426. DOI: 10.1038/328422a0

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Nilsson, O.G., Shapiro, M.L., Gage, F.H., Olton, D.S., Björklund, A. Spatial learning and memory following fimbria-fornix transection and grafting of fetal septal neurons to the hippocampus. (1987) Experimental Brain Research. 67(1):195-215.

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The ability of intrahippocampal grafts of fetal septal-diagonal band tissue, rich in developing cholinergic neurons, to ameliorate cognitive impairments induced by bilateral fimbria-fornix transections in rats was examined in three experiments using the Morris water-maze to test different aspects of spatial memory. Experiment 1. Rats with fimbriafornix lesions received either septal cell suspension grafts or solid septal grafts; normal rats and rats with lesions alone were used as controls. Sixteen weeks after surgery, the rats' spatial learning and memory were tested in the water-maze using a place test, designed to investigate place navigation performance, in which rats learned to escape from the water by swimming to a platform hidden beneath the water's surface. After 5 days of training, the rats were given a spatial probe test in which the platform was removed from the tank to test spatial reference memory. Experiment 2. The same rats used in Exp. 1 were tested in a delayed-match-to-sample, working memory version of the water-maze task. The platform was located in one of two possible locations during each trial, which was composed of 2 swims. If the rat remembered the location of the platform on the 2nd swim of a trial, it should find the platform more quickly on that swim, and thereby demonstrate working memory. Experiment 3. Prior to receiving fimbria-fornix lesions, normal rats were trained in a modification of the water-maze task using alternating cue navigation and place navigation trials (i.e., with visible or non-visible escape platforms). The retention and reacquisition of the place task and the spatial probe test were examined in repeated tests up to 6 months after the lesion and intrahippocampal grafting of septal cell suspensions. The effects of central muscarinic cholinergic receptor blockade with atropine were also tested. Normal rats performed well in both the place and spatial probe tests. In contrast, rats with fimbria-fornix lesions only were unable to acquire or retain spatial information in any test. Instead, these rats adopted a random, non-spatial search strategy, whereby their latencies to find the platform decreased in the place navigation tasks. Sixty to 80% of the rats with septal suspension or solid grafts had recovered place navigation, i.e., the ability to locate the platform site in the tank, in Exp. 1 and 3, and they showed a significantly improved performance in the working memory test in Exp. 2. Atropine abolished the recovered place navigation in the grafted rats, whereas normal rats were impaired to a lesser extent.(ABSTRACT TRUNCATED AT 400 WORDS)

Björklund, A., Gage, F.H. Grafts of fetal septal cholinergic neurons to the hippocampal formation in aged or fimbria-fornix lesioned rats. (1987) Progress in Brain Research. 72:333-42.

Gage, F.H., Buzsaki, G., Nilsson, O., Björklund, A. Grafts of fetal cholinergic neurons to the deafferented hippocampus. (1987) Progress in Brain Research. 71:335-47.

Varon, S., Williams, L.R., Gage, F.H. Exogenous administration of neuronotrophic factors in vivo protects central nervous system neurons against axotomy induced degeneration. (1987) Progress in Brain Research. 71:191-201.

Davis, G.E., Blaker, S.N., Engvall, E., Varon, S., Manthorpe, M., Gage, F.H. Human amnion membrane serves as a substratum for growing axons in vitro and in vivo. (1987) Ciencia. 236(4805):1106-9.

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Peterson, G.M., Williams, L.R., Varon, S., Gage, F.H. Loss of GABAergic neurons in medial septum after fimbria-fornix transection. (1987) Neuroscience Letters. 76(2):140-4.

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Gage, F.H., Björklund, A. Denervation-induced enhancement of graft survival and growth. A trophic hypothesis. (1987) Annals of the New York Academy of Sciences. 495:378-95.

Clarke, D.J., Gage, F.H., Dunnett, S.B., Nilsson, O.G., Björklund, A. Synaptogenesis of grafted cholinergic neurons. (1987) Annals of the New York Academy of Sciences. 495:268-83.

Steinbusch, H.W., Beek, A., Frankhuyzen, A.L., Tonnaer, J.A., Gage, F.H., Björklund, A. Functional activity of raphe neurons transplanted to the hippocampus and caudate-putamen. An immunohistochemical and neurochemical analysis in adult and aged rats. (1987) Annals of the New York Academy of Sciences. 495:169-84.

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Björklund, A., Gage, F.H. Grafts of fetal septal cholinergic neurons to the hippocampal formation in aged or fimbria-fornix-lesioned rats. (1987) Annals of the New York Academy of Sciences. 495:120-37.

Gage, F.H., Björklund, A. Trophic and growth-regulating mechanisms in the central nervous system monitored by intracerebral neural transplants. (1987) Ciba Foundation Symposium. 126:143-59.

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Williams, L.R., Vahlsing, H.L., Lindamood, T., Varon, S., Gage, F.H., Manthorpe, M. A small-gauge cannula device for continuous infusion of exogenous agents into the brain. (1987) Exp. Neurol. 95(3):743-54.

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Fischer, W., Wictorin, K., Björklund, A., Williams, L.R., Varon, S., Gage, F.H. Amelioration of cholinergic neuron atrophy and spatial memory impairment in aged rats by nerve growth factor. (1987) Naturaleza. 329(6134):65-8. DOI: 10.1038/329065a0

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Buzsáki, G., Gage, F.H., Czopf, J., Björklund, A. Restoration of rhythmic slow activity (theta) in the subcortically denervated hippocampus by fetal CNS transplants. (1987) Brain Research. 400(2):334-47.

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Solid pieces of the fetal septal region (SG) or hippocampus (HPC) were implanted in a cavity formed by aspiration of the fimbria fornix (FF) and the overlying cingulate cortex on one side in adult rats. In other lesioned animals cell suspensions obtained from the fetal septal area (SS) or the locus coeruleus region of the brainstem (LC) were injected stereotaxically into the deafferented host hippocampus. Six to 9 months after transplantation the animals had chronic recording electrodes implanted into both hippocampi. EEG and unit activity were monitored during running in a wheel, drinking and immobility. Unilateral fimbria-fornix lesions abolished rhythmic slow activity (RSA or theta, theta) in the ipsilateral hippocampus and no recovery was seen up to 9 months later in either the control FF-lesioned animals or in the rats with LC suspension grafts. Recovery of RSA, however, was observed in all animals with solid septal grafts and in some rats with solid HPC grafts. Similar to normal rats, RSA was present only during running and absent during drinking and sitting still. Coherence measurements of RSA between the transplanted and intact hemispheres resulted in high values (0.70-0.95). Concurrent with RSA, interneurons and granule cells in the host hippocampus fired rhythmically at RSA frequency (6-9 Hz). The depth profile and the antero-posterior distribution of the power of RSA correlated with the amount and distribution of the graft-induced acetylcholinesterase-positive reinnervation of the host hippocampus. In contrast to the animals with solid septal grafts, placed within the FF lesion cavity, the rats with intrahippocampal septal suspension grafts displayed only short duration bursts of RSA, and mainly during immobility. Based on these findings it is suggested that at least a proportion of the RSA 'pacemaker' cells of the host septum survives the transection of the fimbria-fornix fibers and that a graft of fetal septal or hippocampal tissue implanted into the lesion cavity may be capable of relaying this pacemaker activity to the host hippocampus. This effect may be due to the ability of the grafted tissue to promote the regeneration of new, direct or indirect, septo-hippocampal connections across the lesion cavity.

Buzsáki, G., Gage, F.H., Kellényi, L., Björklund, A. Behavioral dependence of the electrical activity of intracerebrally transplanted fetal hippocampus. (1987) Brain Research. 400(2):321-33.

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Williams, L.R., Varon, S., Peterson, G.M., Wictorin, K., Fischer, W., Bjorklund, A., Gage, F.H. Continuous infusion of nerve growth factor prevents basal forebrain neuronal death after fimbria fornix transection. (1986) Actas de la Academia Nacional de Ciencias de los Estados Unidos de América. 83(23):9231-5.

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Clarke, D.J., Gage, F.H., Nilsson, O.G., Björklund, A. Grafted septal neurons form cholinergic synaptic connections in the dentate gyrus of behaviorally impaired aged rats. (1986) Journal of Comparative Neurology. 252(4):483-92. DOI: 10.1002/cne.902520405

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Gage, F.H., Björklund, A. Cholinergic septal grafts into the hippocampal formation improve spatial learning and memory in aged rats by an atropine-sensitive mechanism. (1986) Diario de Neurociencia. 6(10):2837-47.

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Behaviorally impaired old rats were selected from a population of 75 21- to 23-month-old rats on the basis of the place-investigated abilities in the Morris' water-maze task. After the pretransplant test, half of the old impaired rats received bilateral intrahippocampal cell suspension grafts prepared from the fetal septal-diagonal band region, which is rich in developing cholinergic neurons; the other half were left as sham-operated controls. Twelve weeks after grafting, the rats were retested in the water-maze using alternating cue-navigation and place-navigation trials (i.e., with visible or nonvisible escape platform). While the old impaired controls had deteriorated further between the 2 tests and showed poor retention of the pretransplant performance level, the grafted rats were significantly improved in comparison with both their pretransplant performance and the performance of the old impaired controls in the posttransplant test. The graft-induced effect was due both to an improved retention of the performance level acquired in the test performed prior to transplantation surgery, and to an improved acquisition of spatial memory of the escape platform site during the posttransplant test week (measured as an improved focusing of search over the platform site after platform removal). The acetylcholine esterase inhibitor physostigmine had no or only marginal effects on the performance of any of the young or aged rat groups. By contrast, the recovered navigational abilities and spatial memory in the grafted animals were completely abolished after administration of the muscarinic receptor antagonist atropine. A similar atropine-sensitivity was seen in behaviorally nonimpaired aged rats but not in the well-trained young control rats. The results show that cholinergic septal grafts, implanted into the hippocampal formation, improved spatial learning in the impaired aged rats primarily, or perhaps exclusively, by a recovery of the rats' ability to use spatial cues for place-navigation in the water-maze, while other aspects of the performance deficits in the water-maze task in the aged rats were left unaffected by the septal grafts. On the basis of parallel morphological studies, it is proposed that the effects of the septal grafts on the spatial learning impairments in the aged rats are critically dependent on the direct action of cholinergic neurons in the grafts via synapses formed onto neuronal elements in the host by their ingrowing axons.

Gage, F.H., Wictorin, K., Fischer, W., Williams, L.R., Varon, S., Bjorklund, A. Retrograde cell changes in medial septum and diagonal band following fimbria-fornix transection: quantitative temporal analysis. (1986) Neuroscience. 19(1):241-55.

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Complete unilateral fimbria-fornix transections, including the overlying cingulate cortex, were administered to female rats. At time points from 1 day to 6 weeks, the septal-diagonal band region was examined using acetylcholinesterase histochemistry, Cresyl Violet cell staining, and choline acetyltransferase biochemistry. As early as 1 day following the transection a decrease in acetylcholinesterase positive cell body staining was observed in the medial septum; however, no loss of Nissl-stained neurons was measured in Cresyl Violet stained sections until 1 week after the lesion. Maximal loss of acetylcholinesterase-positive cells, as visualized after irreversible acetylcholinesterase inhibition, was measured at 1 week, and no further change was observed at time points up to 6 weeks after operation. The loss of acetyltransferase-positive cells was greatest in the medial septal area (-65%) and the vertical limb of the diagonal band (-55%). Little cell loss was measured in the horizontal limb of the diagonal band. This is consistent with the known projections of these cell bodies. Remaining acetylcholinesterase-positive cell bodies in the medial septum had shrunk by about 20% (measured as the diameter along the major axis). A marked neuronal cell loss (about 50%) was demonstrable in the medial septum and vertical limb of the diagonal band in the Cresyl Violet-stained sections, too. A pile-up of acetylcholinesterase-stained material was observed in the dorsal-lateral quadrant of the septal area just proximal to the lesion at 1 day following transection. This pile-up occurred in the medial septum and diagonal band area up to 1 week following the transection, and had nearly disappeared by 2 weeks post-transection. Choline acetyltransferase biochemical activity, measured in samples of whole septum, decreased significantly at 1 day but subsequently returned to control levels. By 2 weeks following transection, an increase in acetylcholinesterase-positive stained fibers was observed in the dorsal-lateral quadrant of the septum, ipsilateral to the lesion relative to the contralateral septum. This response, which was interpreted as sprouting from the lesioned axons proximal to the transection, probably accounted for the rise in choline acetyltransferase biochemical activity in the whole septum following the reduction on the first day.

Gage, F.H., Björklund, A. Neural grafting in the aged rat brain. (1986) Annual Review of Physiology. 48:447-59. DOI: 10.1146/annurev.ph.48.030186.002311

Björklund, A., Nornes, H., Gage, F.H. Cell suspension grafts of noradrenergic locus coeruleus neurons in rat hippocampus and spinal cord: reinnervation and transmitter turnover. (1986) Neuroscience. 18(3):685-98.

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Fetal noradrenergic neurons from the brain stem locus coeruleus region can be successfully grafted as a dissociated cell suspension provided that the dissociation is done in the absence of any trypsin digestion step. The survival, fiber outgrowth and biochemical function of locus coeruleus neurons, taken from 13- to 15-day-old rat embryos, have been studied after injection into the dorsal hippocampal formation and the thoracolumbar spinal cord in adult rats. All rats were treated with an i.v. injection of 6-hydroxydopamine prior to grafting to remove the intrinsic locus coeruleus projections to these areas, and they were taken for fluorescence histochemical or biochemical analyses 2-7 months after transplantation. Up to 330 surviving noradrenaline neurons were found at each implantation site (injected with 2-3 microliters of cell suspension) which represents an estimated survival rate of about 40%. In the most successful cases the entire dorsal hippocampal formation, and an approximately 4 cm long segment of the thoracolumbar spinal cord, was supplied with a new noradrenaline-containing terminal network, which reached normal densities in the regions closest to the grafts. In the hippocampal formation, in particular, the ingrowing axons re-established a laminar innervation pattern which resembled that of the normal locus coeruleus afferents. In the hippocampus, two 2-microliters injections of locus coeruleus cell suspension restored the total hippocampal noradrenaline content to an average of 55%, and the noradrenaline synthesis rate (as assessed by the rate of DOPA accumulation after synthesis inhibition) was found to be close to normal in the graft-reinnervated specimens. In the spinal cord, two 3-microliters injections restored the noradrenaline level in the thoracolumbar cord (a 4.5 cm long segment) to an average of 22% of normal, with the highest individual levels being close to normal. Determinations of the noradrenaline metabolite 3,4-dihydroxy-phenylethyleneglycol indicated that the rate of noradrenaline metabolism in the graft-reinnervated spinal cord was close to that of the normal intact spinal cord. The results demonstrate the potential of the suspension grafting technique for extensive noradrenergic reinnervation of the hippocampal formation or large portions of the spinal cord. Fetal locus coeruleus neurons implanted in this way can re-establish fairly normal terminal innervation patterns and reinstate noradrenaline turnover and metabolism in a previously denervated central target.

Bengtsson, F., Nobin, A., Falck, B., Gage, F.H., Jeppsson, B. Portacaval shunt in the rat: selective alterations in behavior and brain serotonin. (1986) Pharmacology, Biochemistry, and Behavior. 24(6):1611-6.

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Björklund, A., Gage, F.H. Transplantation of basal forebrain cholinergic neurons in the aged rat brain. (1986) Progress in Brain Research. 70:499-512.

Acsádi, G., Buzsáki, G., Keszthelyi, T., Királyfalvi, L., Gage, F.H. Effects of confinement, previous experience and hippocampal damage on the DRL schedule. (1986) Behavioural Brain Research. 20(2):241-8.

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Lindvall, O., Ingvar, M., Gage, F.H. Short term status epilepticus in rats causes specific behavioral impairments related to substantia nigra necrosis. (1986) Experimental Brain Research. 64(1):143-8.

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Schultzberg, M., Foster, G.A., Gage, F.H., Björklund, A., Hökfelt, T. Coexistence during ontogeny and transplantation. (1986) Progress in Brain Research. 68:129-45.

Kesslak, J.P., Gage, F.H. Recovery of spatial alternation deficits following selective hippocampal destruction with kainic acid. (1986) Behavioral Neuroscience. 100(2):280-3.

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Clarke, D.J., Gage, F.H., Björklund, A. Formation of cholinergic synapses by intrahippocampal septal grafts as revealed by choline acetyltransferase immunocytochemistry. (1986) Brain Research. 369(1-2):151-62.

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Brundin, P., Isacson, O., Gage, F.H., Prochiantz, A., Björklund, A. The rotating 6-hydroxydopamine-lesioned mouse as a model for assessing functional effects of neuronal grafting. (1986) Brain Research. 366(1-2):346-9.

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Kienker, P.K., Sejnowski, T.J., Hinton, G.E., Schumacher, L.E. Separating figure from ground with a parallel network. (1986) Perception. 15(2):197-216. DOI: 10.1068/p150197

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Zetterström, T., Brundin, P., Gage, F.H., Sharp, T., Isacson, O., Dunnett, S.B., Ungerstedt, U., Björklund, A. In vivo measurement of spontaneous release and metabolism of dopamine from intrastriatal nigral grafts using intracerebral dialysis. (1986) Brain Research. 362(2):344-9.

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Gage, F.H., Björklund, A. Enhanced graft survival in the hippocampus following selective denervation. (1986) Neuroscience. 17(1):89-98.

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The trophic effects of denervation on the survival of fetal cholinergic neuronal cell suspensions grafted to the hippocampal formation of the rat were assessed in the present study. Young adult female rats were injected with cell suspensions of neurons obtained from the fetal basal forebrain region into the hippocampal formation simultaneously with (or without) a fimbria-fornix transection, which removes the hippocampal cholinergic afferents. Four to six months later, one group of grafted animals was evaluated histochemically for: transplant volume; number of acetylcholinesterase-positive cells, and size of acetylcholinesterase-positive cells in the graft. A parallel study was conducted to determine the total number and size of the acetylcholinesterase-positive cells in the septal-diagonal band-substantia innominata complex of the adult rat, to match with the cell survival and growth in the grafts. A second group of grafted rats was taken in parallel for biochemical analysis of choline acetyltransferase activity in the grafted hippocampus. The transplant volume in the rats with fimbria-fornix transection was greater than twice the volume seen in animals without fimbria-fornix lesion. In addition, the number of acetylcholinesterase-positive cells in the transplant was twice as great in the denervated animals as in the non-denervated ones. However, the number of acetylcholinesterase-positive cells per mm3 of graft volume did not differ between the two groups, suggesting that the trophic effect of the denervation was not specific for the cholinergic neurons, but affected the entire grafted tissue. The hippocampal choline acetyltransferase activity of the animals that received the fimbria-fornix lesion simultaneously with transplantation was about three times higher than that of the rats that received grafts but no simultaneous fimbria-fornix transection. A control experiment with animals that received an aspirative lesion of the retrosplenial cortex, transecting the perforant path input, revealed no enhancing effect of hippocampal choline acetyltransferase activity over non-lesioned grafted animals. Thus, the denervation-enhancing effects of the fimbria-fornix lesion appear to be selective and not the result of a general wound-induced mechanism. These results strongly support the contention that neurotrophic factors are released as a result of denervation in the adult hippocampal formation, and that these neurotrophic factors can support survival and growth of central cholinergic neurons. However, the factors involved do not appear to be specific for the cholinergic neurons, but rather have their trophic effects on many types of cells.

Brundin, P., Isacson, O., Gage, F.H., Björklund, A. Intrastriatal grafting of dopamine-containing neuronal cell suspensions: effects of mixing with target or non-target cells. (1986) Brain Research. 389(1-2):77-84.

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Isacson, O., Brundin, P., Gage, F.H., Björklund, A. Neural grafting in a rat model of Huntington's disease: progressive neurochemical changes after neostriatal ibotenate lesions and striatal tissue grafting. (1985) Neuroscience. 16(4):799-817.

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The acute and long-term changes following large neostriatal ibotenic acid lesions and intrastriatal striatal neuronal grafting have been studied neurochemically by determinations of the gamma-aminobutyrate (GABA) and acetylcholine-synthetic enzymes, glutamate decarboxylase and choline acetyltransferase, and of dopamine and its primary acidic metabolite, 3,4-dihydroxyphenylacetic acid. The neurochemical data have been matched with estimates of tissue volume changes and striatal graft development through tissue weight and protein content analysis and histological volumetric measurements. Injections of 20 micrograms ibotenic acid, deposited over four injection sites in the head of the caudate-putamen, had by one week caused a 70-85% reduction in glutamate decarboxylase and choline acetyltransferase activity. With time there was a progressive recovery of the specific enzyme activities in the lesioned areas (expressed per microgram protein or per mg wet weight) to 40-60% of control levels as determined at 20 weeks post-lesion in the caudate-putamen. This increase was, however, largely if not exclusively due to the long-term shrinkage of the lesioned caudate-putamen, amounting to 50-70% at 20 weeks. Thus, the total glutamate decarboxylase and choline acetyltransferase activity levels in the lesioned caudate-putamen remained virtually unchanged (between 15 and 25% of control) over the 20 week experimental period. Glutamate decarboxylase activity was also markedly reduced (35-70%) in the two primary striatal projection areas, globus pallidus and substantia nigra, and there were no signs of recovery over time. Striatal dopamine levels, which were acutely unaffected by the lesion, showed a slow decline so that the total dopamine content in the area was reduced by about 80% at 20 weeks. Suspended striatal neurons obtained from the striatal primordia of 14-15-day-old rat fetuses, injected into the previously lesioned caudate-putamen, survived and established a new striatum-like structure at the site of the ibotenic acid lesion. The final volume of the graft tissue reached up to about 10 mm3 in volume and reduced striatal atrophy on average from about 50 to 70% of normal control in the rats with lesions to about 30-40% in the animals with grafts. In the rats with grafts, there was a significant recovery of glutamate decarboxylase and choline acetyltransferase activities not only in the lesioned caudate-putamen, containing the graft (from 20-25% to 40-50%, when expressed as total enzyme activity levels), but also the glutamate decarboxylase activity in the globus pallidus, a structure located at a distance from the graft.(ABSTRACT TRUNCATED AT 400 WORDS)

Pfaff, S.L., Zhou, R.P., Young, J.C., Hayflick, J., Duesberg, P.H. Defining the borders of the chicken proto-fps gene, a precursor of Fujinami sarcoma virus. (1985) Virology. 146(2):307-14.

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The transforming (onc) genes of retroviruses contain specific sequences, derived from as yet poorly defined, normal cellular genes, termed proto-onc genes. Proto-onc genes must be defined to explain their docility compared to the oncogenicity of the viral derivatives. Here we set out to determine the borders of the chicken proto-fps gene from which the onc genes of avian Fujinami (FSV) and PRC sarcoma viruses (PRCSV) are derived. These onc genes are hybrids of an element from the gag gene of retroviruses (delta gag) linked to a 2.8-kb domain from proto-fps. To identify the 5' border of proto-fps we have sequenced 1.5 kb beyond the 5' border of overlap with viral fps utilizing a proto-fps clone derived previously. A possible promoter was identified that maps 736 nucleotides from this border. The 736 nucleotides contain two possible exons with 121 codons, and short regions of homology with the delta gag termini of FSV and PRCII. A translation stop codon and an adjacent polyadenylation signal were identified just prior to the 3' border of overlap with viral fps within a 1.15-kb sequence of a newly isolated proto-fps clone. Comparing four exons within this 1.15 kb proto-fps sequence with known fps equivalents of FSV and PRCSV, we have detected strain-specific, but no common point mutations in each viral genome. A 3.3-kb polyadenylated proto-fps mRNA was detected in chicken liver RNA by gel electrophoresis and hybridization with proto-fps DNA. We conclude that the coding capacity of proto-fps is just over 3 kb, consistent with the size of the putative proto-fps protein of 98 kDa and hence slightly larger than that of viral fps. Thus proto-fps and the viral delta gag-fps genes each contain distinct 5' regulatory and coding sequences and share the 3' terminal fps domains. It is suggested that this difference, rather than scattered point mutations, is responsible for the oncogenic function of the viral genes and the unknown cellular function of proto-fps.

Brundin, P., Barbin, G., Isacson, O., Mallat, M., Chamak, B., Prochiantz, A., Gage, F.H., Björklund, A. Survival of intracerebrally grafted rat dopamine neurons previously cultured in vitro. (1985) Neurosci. Lett. 61(1-2):79-84.

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Bengtsson, F., Gage, F.H., Jeppsson, B., Nobin, A., Rosengren, E. Brain monoamine metabolism and behavior in portacaval-shunted rats. (1985) Experimental Neurology. 90(1):21-35.

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Gage, F.H., Brundin, P., Isacson, O., Björklund, A. Rat fetal brain tissue grafts survive and innervate host brain following five day pregraft tissue storage. (1985) Neuroscience Letters. 60(2):133-7.

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Segal, M., Bjorklund, A., Gage, F.H. Transplanted septal neurons make viable cholinergic synapses with a host hippocampus. (1985) Brain Research. 336(2):302-7.

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Brundin, P., Nilsson, O.G., Gage, F.H., Björklund, A. Cyclosporin A increases survival of cross-species intrastriatal grafts of embryonic dopamine-containing neurons. (1985) Experimental Brain Research. 60(1):204-8.

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Shapiro, M.L., Simon, D., Olton, D.S., Gage, F.H., Bjorklund, A., Stenevi, U. Brain transplants: can they restore single unit activity in the hippocampus? (1985) Annals of the New York Academy of Sciences. 444:536-7.

Foster, G.A., Schultzberg, M., Gage, F.H., Björklund, A., Hökfelt, T., Nornes, H., Cuello, A.C., Verhofstad, A.A., Visser, T.J. Transmitter expression and morphological development of embryonic medullary and mesencephalic raphé neurones after transplantation to the adult rat central nervous system. I. Grafts to the spinal cord (1985) Experimental Brain Research. 60(3):427-44.

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Suspensions of cells derived from the mesencephalic raphé or medullary raphé regions of the 13-14 day old embryonic rat brain were injected into the spinal cord of adult rats which had been previously denervated with 5,7-dihydroxytryptamine. At periods of up to 12 months after grafting, the spinal cords were taken for immunohistochemical analysis of 5-hydroxytryptamine (5HT), substance P (SP) and thyrotropin releasing hormone (TRH). In nearly all cases, surviving transplants were found. The grafts derived from mesencephalic raphé cells contained neurones which were immunoreactive to 5HT, or SP, but not both together. On average 4% of the total possible number of the available embryonic mesencephalic serotoninergic cells were found. A very dense outgrowth of 5HT positive fibres from the transplant was observed, extending up to 1.5 cm in both the caudal and rostral directions from the graft locus. Some SP immunoreactive fibres were also apparent near the implant. The grafts derived from the medullary transplant also contained 5HT-immunoreactive cells, comprising on average 25% of the total 5HT neurones available from the embryonic medullary primordium. In addition, neurones co-localizing 5HT together with SP and TRH were visible, closely reflecting the situation found in the medullary raphé in situ. Dense plexi of fibres containing 5HT-LI extended both caudally and rostrally up to 12-15 mm from the transplant. Outgrowth of SP and TRH varicose fibres was also demonstrable, although to a lesser degree than for 5HT. It was also possible to find many motoneurones surrounded by varicose fibres containing both 5HT and SP, in contrast to the situation with the mesencephalic grafts, where no such patterns of innervation were seen. The experiments indicate that the milieu of the spinal cord may compromise the survival of mesencephalic raphé 5HT neurones far more than of medullary serotonin cells. However, despite this effect on cell survival, the outgrowth of fibres from the remaining mesencephalic 5HT neurones was apparently unaffected by their ectopic position. Similarly, the transmitter content of both classes of raphé cells was largely unaltered, either by the transplantation process or by the environment into which they were placed. It is concluded that although the adult denervated spinal cord can selectively affect neuronal survival, it is incapable either of inducing in other serotoninergic cells placed within it the transmitter phenotype typical of medullary raphé neurones, or of causing those ectopically located 5HT cells to form connections appropriate to the descending serotonin fibres.

Goulding, M., Ralph, R.K. Cyclic AMP, nuclear protein kinase and the PY815 cell cycle. (1985) Molecular and Cellular Biochemistry. 67(1):31-8.

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Wieloch, T., Lindvall, O., Blomqvist, P., Gage, F.H. Evidence for amelioration of ischaemic neuronal damage in the hippocampal formation by lesions of the perforant path. (1985) Neuroscience Letters. 7(1):24-6.

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Kelly, P.A., Gage, F.H., Ingvar, M., Lindvall, O., Stenevi, U., Björklund, A. Functional reactivation of the deafferented hippocampus by embryonic septal grafts as assessed by measurements of local glucose utilization. (1985) Experimental Brain Research. 58(3):570-9.

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Transection of the septo-hippocampal connections through fimbria-fornix damage in the rat results in profound hippocampal cholinergic deafferentation, and, when applied bilaterally, leads to severe and long-lasting impairments in learning and memory. Previous studies have shown that intrahippocampal septal grafts can reestablish a new cholinergic in the initially denervated hippocampal formation and at least partly compensate for the lesion-induced learning impairments in fimbria-fornix lesioned rats. The purpose of the present study was to determine the magnitude of lesion-induced alterations in cerebral function as reflected in local glucose use measured by (14C)-2-deoxyglucose (2-DG) autoradiography, and the degree to which this index of functional activity could be normalized following reinnervation from transplants of fetal cerebral tissue from the primordial septal region. Six months after unilateral fimbria-fornix transection the rate of glucose utilization was reduced markedly throughout the ipsilateral hippocampus when compared to the intact contralateral side, while in the neocortex only the cingulate cortex showed long-lasting reductions in glucose use. Rats that received a transplant of fetal septal-diagonal band tissue at the time of fimbria-fornix transection, and were sacrificed 6 months later, displayed significantly greater glucose utilization in the ipsilateral hippocampus and cingulate cortex than was measured in these areas in rats with lesion alone. The recovery in glucose use was paralleled by a significant increase in acetylcholinesterase (AChE) staining in several areas of the ipsilateral hippocampal formation and cingulate cortex. This index of graft-induced cholinergic reinnervation was, moreover, significantly correlated with the rate of glucose use. Thus, in the fimbria-fornix transected animals the magnitude of glucose depression correlated with the extent of reduction in AChE staining, and in the grafted animals the degree of normalization of glucose use was correlated with the graft-induced increase in AChE-staining density. These results thus indicate that the 2-DG autoradiographic technique can provide a unique opportunity to map both altered functional activity in localized areas of the brain following specific lesions and the extent to which transplant-derived reinnervation of the host may induce a return to normal functional levels in the target site.

Björklund, A., Gage, F.H., Stenevi, U., Dunnett, S.B., Kelly, P.A. Intracerebral neural grafting in animal models of aging brain: strategies, rationale and preliminary results. (1985) Danish Medical Bulletin. 32 Suppl 1:35-9.

Gage, F.H., Stenevi, U., Carlstedt, T., Foster, G., Björklund, A., Aguayo, A.J. Anatomical and functional consequences of grafting mesencephalic neurons into a peripheral nerve "bridge" connected to the denervated striatum. (1985) Exp Brain Res. 60(3):584-9.

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Björklund, A., Gage, F.H. Neural grafting in animal models of neurodegenerative diseases. (1985) Annals of the New York Academy of Sciences. 457:53-81.

Gage, F.H., Kelly, P.A., Björklund, A. Regional changes in brain glucose metabolism reflect cognitive impairments in aged rats. (1984) Diario de Neurociencia. 4(11):2856-65.

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Aged rats (22 to 24 months) and young control rats (3 months) were tested in a battery of behavioral tests which included tests of learning, place navigation, sensorimotor integration, motor coordination, activity, and exploration. Following testing all animals were analyzed in an unanesthetized state for their local glucose utilization. Significant differences in glucose utilization were found between the aged and young groups on some behaviors and in some brain regions. There was considerable variability in the aged group in both their behavioral performance and their glucose utilization scores; thus, attempts were made to determine whether the variability in the degree of impairment within any particular behavioral test was correlated to the regional glucose utilization scores in any of the 45 brain regions analyzed. In two of the behavioral tests employed (i.e., one for learning and one for place navigation), the decline in performance correlated significantly with the decrement in regional glucose utilization. Moreover, the performance in these two tests showed significant correlation with glucose use in only five regions (dentate gyrus, medial septum-diagonal band area, hippocampal CA1, hippocampal CA3, and prefrontal cortex). These results show that the learning impairments in the aged rats are related to the extent of decrease in glucose utilization in restricted areas of the limbic system. In addition, the results show that the individual rats within an aged rat population develop cognitive impairments to a variable degree and that the aged rats with the most pronounced learning impairments are the ones exhibiting the most severe functional decrements, in terms of glucose utilization, in the septohippocampal system and the prefrontal cortex. This suggests that aging rats may be interesting not only for the study of the normal aging process, but also as a model of dementia.

Isacson, O., Brundin, P., Kelly, P.A., Gage, F.H., Björklund, A. Functional neuronal replacement by grafted striatal neurones in the ibotenic acid-lesioned rat striatum. (1984) Naturaleza. 311(5985):458-60.

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Evans, S.H., Chafetz, M.D., Gage, F.H. Taxonomic abstraction in psychobiology. (1984) Journal of General Psychology. 111(2ND Half):209-27. DOI: 10.1080/00221309.1984.9921111

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Gage, F.H., Björklund, A. Intracerebral grafting of neuronal cell suspensions into the adult brain. (1984) Central Nervous System Trauma. 1(1):47-56.

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Gage, F.H., Björklund, A., Stenevi, U., Dunnett, S.B., Kelly, P.A. Intrahippocampal septal grafts ameliorate learning impairments in aged rats. (1984) Ciencia. 225(4661):533-6.

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Dunnett, S.B., Bunch, S.T., Gage, F.H., Björklund, A. Dopamine-rich transplants in rats with 6-OHDA lesions of the ventral tegmental area. I. Effects on spontaneous and drug-induced locomotor activity. (1984) Behavioural Brain Research. 13(1):71-82.

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Gage, F.H., Björklund, A., Stenevi, U. Denervation releases a neuronal survival factor in adult rat hippocampus. (1984) Naturaleza. 308(5960):637-9.

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Gage, F.H., Dunnett, S.B., Björklund, A. Spatial learning and motor deficits in aged rats. (1984) Neurobiology of Aging. 5(1):43-8.

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Dunnett, S.B., Gage, F.H., Björklund, A., Stenevi, U., Low, W.C., Iversen, S.D. Hippocampal deafferentation: transplant-derived reinnervation and functional recovery. (1984) Acta psychiatrica Scandinavica. Supplementum. 313:46-56.

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Gage, F.H., Björklund, A., Stenevi, U. Cells of origin of the ventral cholinergic septohippocampal pathway undergoing compensatory collateral sprouting following fimbria-fornix transection. (1984) Neuroscience Letters. 44(2):211-6.

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Barker, D.J., Howard, A.J., Gage, F.H. Functional significance of sympathohippocampal sprouting: changes in single cell spontaneous activity. (1984) Brain Research. 291(2):357-63.

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Stewart, G.R., Frederickson, C.J., Howell, G.A., Gage, F.H. Cholinergic denervation-induced increase of chelatable zinc in mossy-fiber region of the hippocampal formation. (1984) Brain Research. 290(1):43-51.

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Ballard, D.H., Hinton, G.E., Sejnowski, T.J. Parallel visual computation. (1983) Naturaleza. 306(5938):21-6.

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Kuffler, S.W., Sejnowski, T.J. Peptidergic and muscarinic excitation at amphibian sympathetic synapses. (1983) Journal of Physiology. 341:257-78.

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A single-electrode voltage clamp was used to study the slow muscarinic and late slow peptidergic excitatory post-synaptic currents (e.p.s.c.s) in B cells of the paravertebral sympathetic ganglia of the bull-frog. Conductance decreases were measured during peptidergic e.p.s.c.s in nearly all cells at clamped potentials near the resting level. In about half of the cells the size of the peptidergic e.p.s.c.s increased with hyperpolarization and in some of these cells conductance increases were found at hyperpolarized levels. In the remaining cells conductance decreases occurred at all levels of membrane potential tested, and in a few of these the polarity of the e.p.s.c.s reversed at hyperpolarized potentials. A similar diversity was observed among muscarinic e.p.s.c.s. At least two simple ionic mechanisms are required to explain the heterogeneous voltage dependencies observed: a conductance decrease primarily to K+ that dominates at depolarized potentials and a conductance increase to other ions that is more prominent at hyperpolarized potentials. The proportion of these two mechanisms appears to differ among B cells. The two slow e.p.s.c.s recorded in the same neurone had the same voltage dependence and were accompanied by the same conductance changes in each of eight cells despite differences between cells. The muscarinic e.p.s.c. was reduced during the peptidergic e.p.s.c. in each of twenty-five neurones tested over a range of membrane potentials. Externally-applied luteinizing hormone releasing hormone (LHRH) produced currents with the same voltage dependence and conductance changes as the nerve-evoked peptidergic e.p.s.c. in each of fifteen cells tested. Bethanechol, a muscarinic agonist, and LHRH produced currents with the same voltage dependence and conductance changes in each of the twelve cells studied. In several cells a saturating response to a prolonged application of LHRH completely occluded the response to bethanechol, and vice versa. Slow currents were recorded from dissociated cell bodies in response to bethanechol and LHRH; these responses exhibited the same diversity of voltage dependence and conductance changes as was observed in intact ganglia. Activation of muscarinic and peptidergic receptors may control shared ionic mechanisms in single ganglion cells.

Gage, F.H., Björklund, A., Stenevi, U. Local regulation of compensatory noradrenergic hyperactivity in the partially denervated hippocampus. (1983) Naturaleza. 303(5920):819-21.

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Björklund, A., Stenevi, U., Schmidt, R.H., Dunnett, S.B., Gage, F.H. Intracerebral grafting of neuronal cell suspensions. II. Survival and growth of nigral cell suspensions implanted in different brain sites. (1983) Acta physiologica Scandinavica. Supplementum. 522:9-18.

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Gage, F.H., Björklund, A., Stenevi, U., Dunnett, S.B. Intracerebral grafting of neuronal cell suspensions. VIII. Survival and growth of implants of nigral and septal cell suspensions in intact brains of aged rats. (1983) Acta physiologica Scandinavica. Supplementum. 522:67-75.

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Björklund, A., Gage, F.H., Schmidt, R.H., Stenevi, U., Dunnett, S.B. Intracerebral grafting of neuronal cell suspensions. VII. Recovery of choline acetyltransferase activity and acetylcholine synthesis in the denervated hippocampus reinnervated by septal suspension imp (1983) Acta physiologica Scandinavica. Supplementum. 522:59-66.

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Björklund, A., Gage, F.H., Stenevi, U., Dunnett, S.B. Intracerebral grafting of neuronal cell suspensions. VI. Survival and growth of intrahippocampal implants of septal cell suspensions. (1983) Acta physiologica Scandinavica. Supplementum. 522:49-58.

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Björklund, A., Stenevi, U., Schmidt, R.H., Dunnett, S.B., Gage, F.H. Intracerebral grafting of neuronal cell suspensions. I. Introduction and general methods of preparation. (1983) Acta physiologica Scandinavica. Supplementum. 522:1-7.

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Gage, F.H., Dunnett, S.B., Brundin, P., Isacson, O., Björklund, A. Intracerebral grafting of embryonic neural cells into the adult host brain: an overview of the cell suspension method and its application. (1983) Developmental Neuroscience. 6(3):137-51.

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Gage, F.H., Björklund, A., Stenevi, U., Dunnett, S.B. Functional correlates of compensatory collateral sprouting by aminergic and cholinergic afferents in the hippocampal formation. (1983) Brain Research. 268(1):39-47.

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Selective lesions of discrete aminergic and cholinergic afferent pathways to the hippocampal formation have been studied as an experimental model of behavioral recovery after partial hippocampal deafferentation in adult rats. The noradrenergic, serotoninergic and cholinergic afferents reach the hippocampal formation along three routes: a supracallosal pathway, a subcallosal pathway (along the fimbria-fornix), and a ventral pathway (along the ansa lenticularis and the ventral amygdaloid pathway). Bi-lateral lesion of the supracallosal pathway resulted in significant reductions in biochemical markers of the hippocampal noradrenergic, serotoninergic and cholinergic innervations. Within 6 weeks following lesion these markers were found to have recovered to levels close to normal. A more substantial deafferentation, obtained by a bilateral lesion of both dorsal pathways, resulted in a more profound initial decrease in the biochemical marker levels, and a less complete recovery in the long-term animals. Concomitant with the initial phase of deafferentation, rats with lesions of one or both of the dorsal afferent pathways exhibited an equally profound behavioral deficit in acquisition of a forced choice alternation learning task in a T-maze. However, while rats subjected to the combined supracallosal-fimbria-fornix lesion remained impaired, the ones with lesions of the supracallosal pathway alone exhibited a significant recovery of the capacity to perform the maze task. The correlation between behavioral recovery and recovery of the biochemical markers in the supracallosal pathway-lesioned group was significant for the serotoninergic innervation of the hippocampus. The present results suggest that reinnervation of the partially deafferented hippocampus through compensatory collateral sprouting is a valuable new experimental model system within which to analyze mechanisms underlying functional recovery after lesions in the CNS.

Gage, F.H., Björklund, A., Stenevi, U. Reinnervation of the partially deafferented hippocampus by compensatory collateral sprouting from spared cholinergic and noradrenergic afferents. (1983) Brain Research. 268(1):27-37.

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Whipple, M.R., Reinecke, M.G., Gage, F.H. Inhibition of synaptosomal neurotransmitter uptake by hallucinogens. (1983) Journal of Neurochemistry. 40(4):1185-8.

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Chafetz, M.D., Gage, F.H. Pineal and habenula innervation altered by septal lesions. (1983) Brain Research Bulletin. 10(1):27-31.

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Schmidt, R.H., Björklund, A., Stenevi, U., Dunnett, S.B., Gage, F.H. Intracerebral grafting of neuronal cell suspensions. III. Activity of intrastriatal nigral suspension implants as assessed by measurements of dopamine synthesis and metabolism. (1983) Acta physiologica Scandinavica. Supplementum. 522:19-28.

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Sejnowski, T.J., Yodlowski, M.L. A freeze-fracture study of the skate electroreceptor. (1982) Journal of Neurocytology. 11(6):897-912.

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Sejnowski, T.J. Peptidergic synaptic transmission in sympathetic ganglia. (1982) Federation Proceedings. 41(13):2923-8.

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Turner, F.D., Gage, F.H. Behavioral kinetics: dynamics of the unconditioned response to footshock. (1982) Physiology & Behavior. 29(5):957-60.

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Paton, J.A., Kelley, D.B., Sejnowski, T.J., Yodlowski, M.L. Mapping the auditory central nervous system of Xenopus laevis with 2-deoxyglucose autoradiography. (1982) Brain Research. 249(1):15-22.

Chafetz, M.D., Evans, S., Gage, F.H. Fluorescence measurement of lesion-induced fiber growth. II. Measurement of sympathohippocampal sprouting using a new microfluorometric method. (1982) Brain Research. 247(2):217-25.

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Chafetz, M.D., Gage, F.H. Fluorescence measurement of lesion-induced fiber growth. I. A microfluorometric technique to measure density and intensity of varicosities and fibers. (1982) Brain Research. 247(2):209-16.

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Björklund, A., Stenevi, U., Dunnett, S.B., Gage, F.H. Cross-species neural grafting in a rat model of Parkinson's disease. (1982) Naturaleza. 298(5875):652-4.

Dunnett, S.B., Gage, F.H., Björklund, A., Stenevi, U., Low, W.C., Iversen, S.D. Hippocampal deafferentation: transplant-derived reinnervation and functional recovery. (1982) Scandinavian Journal of Psychology. Suppl 1:104-11.

Gage, F.H., Dunnett, S.B., Björklund, A., Stenevi, U. Functional recovery following brain damage: conceptual frameworks and biological mechanisms. (1982) Scandinavian Journal of Psychology. Suppl 1:112-20.

Thompson, R.G., Valdes, J.J., Gage, F.H. Reversible alterations in hypothalamic uptake of biogenic amines after septal lesions. (1981) Experimental Neurology. 74(2):356-69.

Gage, F.H., Springer, J.E. Behavioral assessment of norepinephrine and serotonin function and interaction in the hippocampal formation. (1981) Pharmacology, Biochemistry, and Behavior. 14(6):815-21.

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Chafetz, M.D., Thompson, R.G., Evans, S.H., Gage, F.H. Biochemical specificity of septal hyperreactivity: a behavioral discrimination. (1981) Behavioural Brain Research. 2(3):409-20.

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Reingold, S.C., Sejnowski, T.J., Gelperin, A., Kelley, D.B. [3H]-2-deoxyglucose autoradiography in a molluscan nervous system. (1981) Brain Research. 208(2):416-20.

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Gage, F.H., Thompson, R.G. Differential distribution of norepinephrine and serotonin along the dorsal-ventral axis of the hippocampal formation. (1980) Brain Research Bulletin. 5(6):771-3.

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Sejnowski, T.J., Reingold, S.C., Kelley, D.B., Gelperin, A. Localization of[3H]-2-deoxyglucose in single molluscan neurones. (1980) Naturaleza. 287(5781):449-51.

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Gage, F.H., Valdes, J.J., Thompson, R.G. Morphine injections into the periaqueductal-periventricular gray attenuate septal hyperreactivity. (1980) Psychopharmacology. 70(1):113-4.

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Valdes, J.J., Cameron, W.R., Evans, S., Gage, F.H. Regional brain morphine injections selectively attenuate aspects of septal hyperreactivity: a multivariate assessment. (1980) Pharmacology, Biochemistry, and Behavior. 12(4):563-72.

Gage, F.H., Armstrong, D.R., Thompson, R.G. Behavioral kinetics: a method for deriving qualitative and quantitative changes in sensory responsiveness following septal nuclei damage. (1980) Physiology & Behavior. 24(3):479-84.

Cameron, W.R., Gage, F.H., Hitt, J.C., Popolow, H.B. The influence of the anterodorsal hippocampus on feminine sexual behavior in rats. (1979) Behavioral and Neural Biology. 27(1):72-86.

Babitch, J.A., Gage, F.H., Valdes, J.J. Effects of phalloidin on K+-dependent, Ca2+-independent neurotransmitter efflux and K+-facilitated, Ca2+-dependent neurotransmitter release. (1979) Life Sciences. 24(2):117-24.

Gage, F.H., Thompson, R.G., Valdes, J.J. Endogenous norepinephrine and serotonin within the hippocampal formation during the development and recovery from septal hyperreactivity. (1978) Pharmacology, Biochemistry, and Behavior. 9(3):359-67.

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Gage, F.H., Olton, D.S., Bolanowski, D. Activity, reactivity, and dominance following septal lesions in rats. (1978) Behavioral Biology. 22(2):203-10.

Olton, D.S., Walker, J.A., Gage, F.H. Hippocampal connections and spatial discrimination. (1978) Brain Research. 139(2):295-308.

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Sejnowski, T.J. Statistical constraints on synaptic plasticity. (1977) Journal of Theoretical Biology. 69(2):385-9.

Sejnowski, T.J. Storing covariance with nonlinearly interacting neurons. (1977) Journal of Mathematical Biology. 4(4):303-21.

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Johnson, C.T., Olton, D.S., Gage, F.H., Jenko, P.G. Damage to hippocampus and hippocampal connections: effects on DRL and spontaneous alternation. (1977) Journal of Comparative and Physiological Psychology. 91(3):508-22.

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Jenko, P.G., Gage, F.H., Olton, D.S. Time-dependent fornix influence on increased light aversion induced by septal lesions. (1976) Behavioral Biology. 17(4):607-14.

Gage, F.H., Olton, D.S. L-Dopa reduces hyperreactivity induced by septal lesions in rats. (1976) Behavioral Biology. 17(2):213-8.

Sejnowski, T.J. On the stochastic dynamics of neuronal interaction. (1976) Biological Cybernetics. 22(4):203-11.

Sejnowski, T.J. On global properties of neuronal interaction. (1976) Biol Cybern. 22(2):85-95.

Gage, F.H., Olton, D.S. Hippocampal influence on hyperreactivity induced by septal lesions. (1975) Brain Research. 98(2):311-25.

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Olton, D.S., Gage, F.H. Role of the fornix in the septal syndrome. (1974) Physiology & Behavior. 13(2):269-79.

Woodruff, M.L., Gage, F.H., Isaacson, R.L. Changes in focal epileptic activity produced by brain-stem sections in the rabbit. (1973) Electroencephalography and Clinical Neurophysiology. 35(5):475-86.

Isaacson, R.L., Gage, F.H. Hypothalamic stimulation and the persistence of epileptiform discharges. (1973) Brain Research. 49(1):194-9.

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