{"id":32805,"date":"2021-11-21T18:35:55","date_gmt":"2021-11-22T02:35:55","guid":{"rendered":"https:\/\/vermont.salk.edu\/?page_id=32805"},"modified":"2024-02-02T21:57:58","modified_gmt":"2024-02-03T05:57:58","slug":"sequencing-and-single-cell-analysis","status":"publish","type":"page","link":"https:\/\/www.salk.edu\/zh\/science\/research-centers\/san-diego-nathan-shock-center\/research-resources\/sequencing-and-single-cell-analysis\/","title":{"rendered":"\u6d4b\u5e8f\u4e0e\u57fa\u56e0\u7ec4\u670d\u52a1"},"content":{"rendered":"<p><strong>1) \u6279\u91cf\u6d4b\u5e8f\u7684\u6587\u5e93\u5236\u5907<\/strong><\/p>\n<p style=\"padding-left: 40px;\">\u6211\u4eec\u63d0\u4f9b\u4ee5\u4e0b\u7528\u4e8e\u6279\u91cf RNA \u548c DNA \u6d4b\u5e8f\u7684\u6587\u5e93\u5236\u5907\u670d\u52a1\uff1a<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Illumina \u94fe\u5f0f mRNA \u6587\u5e93<\/li>\n<li>Illumina\u5168RNA\u6d4b\u5e8f\u6587\u5e93<\/li>\n<li>Nextera XT\/Flex DNA \u6587\u5e93<\/li>\n<li>NEB \u67d3\u8272\u8d28\u514d\u75ab\u6c89\u6dc0\u6d4b\u5e8f\u6587\u5e93\u5236\u5907<\/li>\n<li>Takara \u8d85\u4f4e\u8f93\u5165RNA\u6d4b\u5e8fcDNA\u6587\u5e93\u6784\u5efa<\/li>\n<li>\u65e0 PCR \u6837\u54c1\u5236\u5907<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>2) \u5355\u7ec6\u80de\/\u7ec6\u80de\u6838\u6d4b\u5e8f\u6837\u672c\u5236\u5907\uff08\u4ec5\u9650\u672c\u5730\u83b7\u5956\u8005\uff09<\/strong><\/p>\n<p style=\"padding-left: 40px;\">\u6211\u4eec\u4e3a\u5355\u7ec6\u80de\u548c\u5355\u6838\u6d4b\u5e8f\u63d0\u4f9b\u4ee5\u4e0b 10x Genomics \u6587\u5e93\u5236\u5907\u670d\u52a1\uff1a<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>\u5355\u7ec6\u80de 3\u2019 v3.1<\/li>\n<li>\u5355\u7ec6\u80de ATAC<\/li>\n<li>\u5355\u7ec6\u80de\u591a\u7ec4\u5b66<\/li>\n<li>\u5355\u7ec6\u80de 5\u2019 VDJ v2<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>3) \u4e0b\u4e00\u4ee3\u6d4b\u5e8f<\/strong><\/p>\n<p style=\"padding-left: 40px;\">\u6211\u4eec\u63d0\u4f9b\u5e7f\u6cdb\u7684\u6d4b\u5e8f\u670d\u52a1\uff0c\u6240\u7528\u7684 Illumina \u6d4b\u5e8f\u4eea\u5305\u62ec\uff1a<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>NovaSeq 6000<\/li>\n<li>NextSeq 2000<\/li>\n<li>MiniSeq<\/li>\n<li>MiSeq<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>4) \u4f7f\u7528nCounter MAX\u8fdb\u884c\u6570\u5b57\u57fa\u56e0\u8868\u8fbe<\/strong><\/p>\n<p style=\"padding-left: 40px;\">\u6211\u4eec\u901a\u8fc7\u4f7f\u7528 NanoString \u6280\u672f\uff0c\u4e3a\u4f4e\u4ea7\u91cf\u548c\/\u6216\u964d\u89e3\u7684 RNA \u6837\u672c\u63d0\u4f9b\u5f3a\u5927\u7684\u6570\u5b57\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u3002 <a href=\"https:\/\/nanostring.com\/products\/ncounter-analysis-system\/ncounter-systems-overview\/?utm_source=google&amp;utm_medium=paidsearch&amp;utm_campaign=dynamic&amp;utm_id=NSTG_DynamicSearch&amp;gad=1&amp;gclid=CjwKCAjwxr2iBhBJEiwAdXECw7wFzBzxrcn4DO6uLrT0DSn-mEirupKGjHi30eD9SKxCHC3xNHoQbRoCFDUQAvD_BwE\">nCounter MAX \u5206\u6790\u7cfb\u7edf<\/a> \u8fde\u540c <a href=\"https:\/\/nanostring.com\/products\/ncounter-assays-panels\/\">\u57fa\u56e0\u68c0\u6d4b\u7ec4\u5408<\/a> \u53ef\u9776\u5411\u591a\u8fbe 800 \u4e2a\u57fa\u56e0\u3002.<\/p>\n<p><strong>5) \u5b8c\u6574\u7684\u7ec4\u7ec7\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\uff0c\u4f7f\u7528 NanoString GeoMx \u6570\u5b57\u7a7a\u95f4\u5256\u9762\u4eea\uff082024\u5e74\u63a8\u51fa\uff09 <\/strong><\/p>\n<p><em>\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u6838\u5fc3\u8054\u7cfb\u65b9\u5f0f\uff1a <\/em><a href=\"mailto:samplesubmission@salk.edu\"><em>samplesubmission@salk.edu<\/em><\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>1) \u6279\u91cf\u6d4b\u5e8f\u7684\u6587\u5e93\u5236\u5907<\/strong><\/p>","protected":false},"author":91,"featured_media":0,"parent":28632,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"folder":[510],"class_list":["post-32805","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Sequencing and Genomic Services - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/zh\/science\/research-centers\/san-diego-nathan-shock-center\/research-resources\/sequencing-and-single-cell-analysis\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta 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