{"id":10754,"date":"2016-08-25T00:00:02","date_gmt":"2016-08-25T07:00:02","guid":{"rendered":"https:\/\/vermont.salk.edu\/?post_type=disclosure&p=10754"},"modified":"2024-01-30T15:16:10","modified_gmt":"2024-01-30T23:16:10","slug":"new-method-creates-endless-supply-kidney-precursor-cells","status":"publish","type":"disclosure","link":"https:\/\/www.salk.edu\/zh\/news-release\/new-method-creates-endless-supply-kidney-precursor-cells\/","title":{"rendered":"New method creates endless supply of kidney precursor cells"},"content":{"rendered":"
\"Image-#1-hr\"
Salk researchers created kidney progenitor cells that survive in the lab using 3D culture and a mix of supporting molecules. The technology could help point to new cell therapies.<\/p>\n

Click here<\/a> for a high-resolution image <\/p>\n

Credit: Salk Institute<\/figcaption><\/figure>\n

LA JOLLA\u2014Salk Institute scientists have discovered the holy grail of endless youthfulness\u2014at least when it comes to one type of human kidney precursor cell. Previous attempts to maintain cultures of the so-called nephron progenitor cells often failed, as the cells died or gradually lost their developmental potential rather than staying in a more medically useful precursor state.<\/p>\n

But by using a three-dimensional culture and a new mixture of supporting molecules, Salk researchers have successfully suspended the cells early in their development. Such early-stage kidney cells could be used to grow replacement kidney tissue in order to study the organ as well as treat disease.<\/p>\n

\u201cWe provide a proof-of-principle for how to make and maintain unlimited numbers of precursor kidney cells,\u201d says Juan Carlos Izpisua Belmonte<\/a>, professor in Salk\u2019s Gene Expression Laboratory. \u201cHaving a supply of these cells could be a starting point to grow functional organs in the laboratory as well as a way to begin applying cell therapy to kidneys with malfunctioning genes.\u201d The work was published in Cell Stem Cell<\/a><\/i> on August 25, 2016.<\/p>\n

Nephron progenitor cells (NPCs), at least in humans, normally only exist during a brief stage of embryonic development. The cells go on to form nephrons, the functional units of the kidney, responsible for filtering the blood and excreting urine. But adults have no remaining NPCs to grow new kidney tissue after damage or disease. Generating NPCs in the lab, scientists believe, will offer a new way to study kidney development and eventually treat kidney diseases.<\/p>\n

\"Kidney\"
Salk scientists created a new method to develop endless supply of kidneyprecursor cells. Pictured are long-term cultured nephron progenitor cells (red) incorporating and becoming part of the kidney after transplanted into a developing kidney.<\/p>\n

Click here<\/a> for a high-resolution image <\/p>\n

Credit: Salk Institute<\/figcaption><\/figure>\n

Previously, other groups of scientists have used induced pluripotent stem cells (iPSCs) to make NPC-like cells. \u201cThose approaches take a long time, it is difficult to isolate a pure population, and the NPC-like cells are still transient,\u201d says Zhongwei Li, a research associate in the Izpisua Belmonte lab and co-first author of the new paper. In those cases, the NPCs often matured into adult kidney cells in a manner of days, leaving no steady population of progenitor cells to study.<\/p>\n

First working with NPCs directly isolated from mouse embryos, Izpisua Belmonte, Li and collaborators worked to develop methods that would keep the NPCs in their usually transient, progenitor state. They discovered that if they maintained the cells in a three-dimensional culture, rather than a flat dish, and used a new mixture of signaling molecules, they could maintain the NPCs for more than 15 months. They went on to show that the cells\u2014when moved to new conditions\u2014could then be coaxed to develop into functional nephron-like structures both in the lab or when transplanted into animals.<\/p>\n

Next, the team used both human embryonic NPCs and human NPCs generated from stem cells to tweak the protocol for human use. Again, they were able to maintain the NPCs long term.<\/p>\n

“The 3D culture strategy used in our study can potentially be applied to other lineage progenitors for efficient formation of tissue organoids,\u201d says co-first author Jun Wu, Salk research associate.<\/p>\n

\"Zhongwei
Zhongwei Li and Juan Carlos Izpisua Belmonte<\/p>\n

Click here<\/a> for a high-resolution image<\/p>\n

Credit: Salk Institute<\/figcaption><\/figure>\n

Aside from a regenerative therapy to replace ailing organs, the scientists add that the NPCs could be used to model diseases in the lab. By introducing disease related mutations to the cells, researchers could study the onset and progression of the disease and gain new insights into the disease as well as screen and discover new treatment drugs.<\/p>\n

Next, the researchers would like to investigate how to culture the other types of progenitor cells that are required for a full kidney, in addition to the nephrons formed by NPCs. \u201cThere are several progenitor cells that work together to make a whole organ,\u201d adds co-first author Toshikazu Araoka, Salk research associate. \u201cIf we can culture the other progenitor cells as well, we\u2019ll be closer to building a transplantable kidney.\u201d<\/p>\n

Other researchers on the study were Hsin-Kai Liao, Mo Li, Min-Zu Wu, Isao Tamura, Yun Xia, Ergin Beyret, and Concepcion Rodriguez Esteban of the Salk Institute; Marta Lazo and Josep M. Campistol of the Hospital Clinic of Barcelona<\/a>; Bing Zhou and Yinghui Sui of the University of California, San Diego<\/a>; Taji Matsusaka of Tokai University School of Medicine<\/a>; Ira Pastan of the National Cancer Institute<\/a>; Isabel Guillen and Pedro Guillen of Clinica Cemtro in Madrid<\/a>.<\/p>\n

The work and the researchers involved were supported by grants from the Kyoto University Foundation, the Nagai Foundation Tokyo<\/a>, the Kidney Foundation Japan, the California Institute for Regenerative Medicine<\/a>, a Catharina Foundation fellowship, the Universidad Cat\u00f3lica San Antonio de Murcia (UCAM)<\/a>, Fundacion Dr. Pedro Guillen<\/a>, The G. Harold and Leila Y. Mathers Charitable Foundation<\/a>, The Leona M. and Harry B. Helmsley Charitable Trust<\/a>, and The Moxie Foundation<\/a>.<\/p>","protected":false},"featured_media":10756,"template":"","faculty":[85],"disease-research":[335],"class_list":["post-10754","disclosure","type-disclosure","status-publish","has-post-thumbnail","hentry","faculty-juan-carlos-izpisua-belmonte","disease-research-regeneration"],"acf":[],"yoast_head":"\nNew method creates endless supply of kidney precursor cells - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/zh\/news-release\/new-method-creates-endless-supply-kidney-precursor-cells\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"New method creates endless supply of kidney precursor cells - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"Salk researchers created kidney progenitor cells that survive in the lab using 3D culture and a mix of supporting molecules. 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