THE SALK INSTITUTE
Q-PCR Facility, ext. 1731
Quant PCR Request Form

Name_________________________________	Date Submitted________________	
Lab/Dept.______________________________		
Ext.__________________________________	Page _____ of _____	
Account number________________________		
*IMPORTANT: In order to get the accurate and consistent reading, please keep the bottom of the plate clean at all times.
Please circle one for the following each choice:

Experiment Design: 
            AQ (standard curve method) 
            RQ (comparative Ct method)

Type of plate:                                 
            384                                              
            96                                                
            TLDA                                         
                           
Template:                                     
            TaqMan (Fast/Regular)
            SYBR Green (Fast/Regular) with melting curve
            SYBR Green (Fast/Regular) without melting curve
            TaqMan Low Density Array (microfluidics card)
         
Ramping Speed:   
      Fast: For using ABI Fast TaqMan and ABI Fast SYBR Green.
                             95°C for 20 seconds
                             95°C for 1 second, 60°C for 20 second, 40 CYCLES
           
      Standard: For using any TaqMan and Sybr includes ABI Power Sybr.
                              50°C for 2 minutes, 95°C for 10 minutes
                              95°C for 15 seconds, 60°C for 1 minute, 40 CYCLES

      9600 Emulation: To mimic the performance of 7700.

            Custom: Please indicate your desired thermal Cycle condition.

Rxn volume: ___________________		
	
Run date: _______________