Peptide Synthesis Core

Scheduling & Rates

Salk Institute for Biological Studies - Peptide Synthesis Core - Scheduling & Rates

Scheduling & Rates

In order to provide this service, the Institute has purchased an automated peptide synthesizer with real-time UV monitoring, The Tribute, from Protein Technologies. This is a cartridge-based system. The amino acid cartridges cost between $5 and $15 for each amino acid, most being in the $6 to $8 range. The synthesizer can run at 3 scales: 100, 300 or 500 uM.

Because the cost of an empty cartridge itself is around $5 , and Fmoc-amino acids are inexpensive, the cost differences between scales are not linear.

For example, a 14 amino acid peptide GEQLHDLNAPRKED (1620.7 MW) :

scale cost Yield (crude) Price/mg
100 $ 88.78 162 mg $ 0,55
300 $114.08 486 mg $ 0.23
500 $136.67 810 mg $ 0.17

The Cancer Center keeps the cost of peptides low by paying for the resin on which the peptides are synthesized, the chemicals used in the syntheses, and for the chemicals used in the deprotection and cleavage of the peptide from the resin post-synthesis. It also provides salary support for the operator of the synthesizer (currently Jill Meisenhelder in Tony Hunter’s lab).

Investigators are recharged for the amino acid cartridges used for their synthesis, and for their share of biotin, specialty, and phosphoamino acids stocked by the Core. The investigator is also recharged for mass spec analyses of the crude ($30); an analytical HPLC ($30); when HPLC purification is necessary, the investigator is recharged $240 for purification of up to 1 g peptide.