BD Influxes (Jet-in-Air technology)
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details go here
The Becton-Dickinson FACSCanto II™ cytometer is an easy to operate 3-laser (8 colour) analytical flow cytometer.
The patented optical design features fiber optics and a gel coupled cuvette flow cell. Fluorescent signals are collected using highly efficient octagon/trigon collection detector arrays that use serial light reflections to guide signals to their target detectors, resulting in highly efficient light collection and providing maximum signal retention at the detector level. This BD serial reflective design further enhances instrument sensitivity by collecting the dimmest emission signals first, moving from the longest wavelengths (typically PE-Cy™7) to the shortest (FITC).to maximize signal detection, increasing sensitivity and resolution. The sample injection tube features a rinse function that automatically washes down the probe between tubes, to reduce sample cross contamination.
The cytometer runs BD FACSDiva acquisition software.
The Becton-Dickinson LSR II is a 5-laser 15-color analytical flow cytometer. The cytometer runs BD FACSDiva acquisition software.
Please contact staff if your fluorochome cannot be accommodated by the standard filters shown; we have other filters in our collection.
|Laser Name||Detector||Mirror||Filter||Diva Names||Use also for these fluors (non exhaustive list):|
|488nm||A||670 LP||692/40 BP||PerCP-Cy5-5|
|50mW||B||505 LP||525/50 BP||FITC or GFP||YFP, Zombie-Green, JC1 [monomeric], pHrodo [green], BB515, Sytox Green|
|640nm||A||750 LP||780/60 BP||APC-Cy7||APC-Cy7, Zombie NIR|
|40mW||B||685 LP||730/45 BP||Alexa Fluor 700||AF680, Draq7|
|561nm||A||750 LP||780/60 BP||PE-Cy7|
|100mW||B||600 LP||615/45 BP||PE-TR, mCherry, mKate, 7-AAD, PI||PE-Dazzle, Texas-Red, Live Dead Red|
|C||582/15 BP||PE, tdTomato, AF594, dsRed, RFP||TMRE, TMRM, JC-1 [aggregated]|
|20mW||B||685 LP||730/45 BP||BUV737|
|C||440 LP||450/50 BP||Indo-1 (Violet), Z-UV||Hoechst (cell cycle), Zombie UV, Indo-1[unbound]|
|D||380/30 BP||Indo-1 (Blue), BUV395||Indo-1[bound]|
|405nm||A||750 LP||780/60 BP||BV785|
|100mW||B||685 LP||730/45 BP||BV711|
|C||655 LP||660/20 BP||BV650|
|D||600 LP||610/20 BP||BV605|
|E||495 LP||510/50 BP||BV510, mCFP|
|F||450/50 BP||DAPI, Pacific Blue, BV421||DAPI or Hoechst (viability), Zombie Violet, BFP|
The Becton-Dickinson Influx™ cytometer is a state-of-the-art, 13 parameter 5-laser high-speed jet-in-air cell sorter.
To achieve very sensitive, low noise electronics, the BD Influx system uses analog electronics in the first stage of signal amplification. The output of the analog peak and hold circuitry is digitized using 16-bit ADCs and placed on a patented parallel processing digital bus. The bus handles real-time compensation as well as other digitized signals, including area and width of up to 8 additional pulses.
The Influx has several nozzle sizes available to accommodate a variety of particle sizes and can sort 6 populations simultaneously (for suitable sample types). Cells may be sorted into a variety of tube types or multiwell plates (e.g. 384 well).
Additionally, the BD Influx™ is equipped with an aerosol management option; air is drawn away from the operator through a HEPA filter, reducing the amount of aerosols in the sort chamber for an added level of safety.