{"id":51960,"date":"2024-12-17T21:24:15","date_gmt":"2024-12-18T05:24:15","guid":{"rendered":"https:\/\/www.salk.edu\/?page_id=51960"},"modified":"2025-12-01T11:48:40","modified_gmt":"2025-12-01T19:48:40","slug":"instrumentation-and-services","status":"publish","type":"page","link":"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/instrumentation-and-services\/","title":{"rendered":"Instrumentaci\u00f3n y servicios"},"content":{"rendered":"<style>\r\n  table {\r\n    border-collapse: collapse;\r\n    width: 100%;\r\n    font-family: Arial, sans-serif;\r\n    border: 1px solid #000;\r\n  }\r\n\r\n  th, td {\r\n    border: 1px solid #000;\r\n    padding: 8px;\r\n    vertical-align: bottom;\r\n  }\r\n\r\n  thead th {\r\n    background: #002060; \/* Dark Blue *\/\r\n    color: #fff; \/* White text *\/\r\n    font-weight: bold;\r\n    text-align: left;\r\n  }\r\n\r\n  tbody td {\r\n    background: #B8CCE4; \/* Light Blue body *\/\r\n    color: #000; \/* Dark text *\/\r\n  }\r\n\r\n.instruction {\r\n  background-color: #fff8e1; \/* a soft yellow pastel *\/\r\n  padding: 1em;\r\n  border-radius: 4px;\r\n  border: 1px solid #ffe0b2;\r\n  font-weight: bold;\r\n}\r\n<\/style>\r\n<a class=\"openall btn -expand\">Expand All<\/a><a class=\"closeall btn -expand\">Collapse All<\/a>\r\n<div id=\"accordion2\" class=\"accordion\" style=\"margin-top: 20px;\">\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed\" href=\"#collapseOne\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Self Service_Shared Instruments<\/a><\/div>\r\n<div id=\"collapseOne\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p>Core Provide training for all shared instruments, and they are operated under Self Service.\r\nPlease use this link <a href=\"https:\/\/ppms.us\/salk\/listsys\/?pf=9\">https:\/\/ppms.us\/salk\/listsys\/?pf=9<\/a> to book those instruments on PPMS \r\n<\/p>\r\n\r\n<div id=\"accordion2\" class=\"accordion\">\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-1\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Agilent TapeStation 4150<\/a><\/div>\r\n\r\n<div id=\"collapseInner-1\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p>Agilent TapeStation 4150 for measuring DNA<\/p>\r\n\r\n <table class=\"table table-bordered\">\r\n  <thead>\r\n    <tr>\r\n      <th>Nucleic Acid<\/th>\r\n      <th>Tape Station Assay<\/th>\r\n      <th>Description<\/th>\r\n      <th>Concentration Range (ng\/\u03bcl)<\/th>\r\n      <th>Size Range (bp)<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td rowspan=\"2\">RNA<\/td>\r\n      <td>RNA Tape<\/td>\r\n      <td>RNA Screen Tape<\/td>\r\n      <td>5-500<\/td>\r\n      <td>50-600<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>HS RNA Tape<\/td>\r\n      <td>High Sensitivity RNA Screen Tape<\/td>\r\n      <td>0.1-10<\/td>\r\n      <td>50-600<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td rowspan=\"3\">DNA<\/td>\r\n      <td>DNA Tape<\/td>\r\n      <td>D1000\/D5000 Screen Tape<\/td>\r\n      <td>0.1-50<\/td>\r\n      <td>35-1000<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>HS DNA Tape<\/td>\r\n      <td>High Sensitivity D1000\/D5000 Screen Tape<\/td>\r\n      <td>0.01-1<\/td>\r\n      <td>35-1000<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>Genomic Tape<\/td>\r\n      <td>Genomic DNA Screen Tape<\/td>\r\n      <td>10-100<\/td>\r\n      <td>200-15000<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 1 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-2\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Qubit 3.0 Fluorometer<\/a><\/div>\r\n\r\n<div id=\"collapseInner-2\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p>Qubit 3.0 Fluorometer for measure DNA or RNA concentration with all the reagents provided. Remember to sign out the reagents usages when finished.<\/p>\r\n\r\n<table class=\"table table-bordered\">\r\n  <thead>\r\n    <tr>\r\n      <th>Nucleic Acid<\/th>\r\n      <th>Qubit Assay<\/th>\r\n      <th>Description<\/th>\r\n      <th>Concentration Range (ng\/\u03bcl)<\/th>\r\n      <th>Sample Types<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <!-- RNA Rows -->\r\n    <tr>\r\n      <td rowspan=\"2\">RNA<\/td>\r\n      <td>BR RNA<\/td>\r\n      <td>Qubit Broad Range RNA<\/td>\r\n      <td>5-1000<\/td>\r\n      <td>RNA<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>HS RNA<\/td>\r\n      <td>Qubit High Sensitivity RNA<\/td>\r\n      <td>0.25-100<\/td>\r\n      <td>RNA from limited samples<\/td>\r\n    <\/tr>\r\n\r\n    <!-- DNA Rows -->\r\n    <tr>\r\n      <td rowspan=\"2\">DNA<\/td>\r\n      <td>BR DNA<\/td>\r\n      <td>Qubit Broad Range DNA<\/td>\r\n      <td>0.1-1000<\/td>\r\n      <td>Genomic, dsDNA<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>HS DNA<\/td>\r\n      <td>Qubit High Sensitivity DNA<\/td>\r\n      <td>0.1-100<\/td>\r\n      <td>dsDNA, ChIP<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 2 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-3\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">QuantStudio\u202f3 and 5 Real-Time PCR Systems<\/a><\/div>\r\n<div id=\"collapseInner-3\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n\r\n<div class=\"accordion-inner\">\r\n<p>QuantStudio\u202f3 and 5 Real-Time PCR Systems can detect differences in target quantity as low as 1.5-fold, support 96-well (0.2ml or 0.1ml) or 384-well (QS5 only) consumables. The recharge is linked to the time it was booked.<\/p>\r\n\r\n<ul>\r\n<li><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2017\/11\/Gene-Expression-SYBR-QS35.pdf\" rel=\"noopener\" target=\"_blank\">Gene Expression SYBR QS35.pdf<\/a><\/li>\r\n<li><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2017\/11\/Gene-Expression-Taqman-QS35.pdf\" rel=\"noopener\" target=\"_blank\">Gene Expression Taqman QS35.pdf<\/a><\/li>\r\n<li><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2017\/11\/Standard-Curve-SYBR-QS35.pdf\" rel=\"noopener\" target=\"_blank\">Standard Curve SYBR QS35.pdf<\/a><\/li>\r\n<li><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2017\/11\/Standard-Curve-Taqman-QS35.pdf\" rel=\"noopener\" target=\"_blank\">Standard Curve Taqman QS35.pdf<\/a><\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 3 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-4\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Tecan Infinite\u00ae 200 Plate reader<\/a><\/div>\r\n<div id=\"collapseInner-4\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n\r\n<div class=\"accordion-inner\">\r\n<p><strong>Tecan Infinite\u00ae 200 Plate reader<\/strong> for measuring fluorescence, luminescence or absorbance detection with an advanced quad-4 monochromators&#x2122; system (M200). It has the i-control&#x2122; software for easy set up protocols, and optional Magellan&#x2122; software packages provide full data reduction capability. It is no charge for using it if you set it up with your own consumables.<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 4 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-5\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">EpiShear Probe Sonicator<\/a><\/div>\r\n\r\n<div id=\"collapseInner-5\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2025\/12\/EpiShear.pdf\" target=\"_blank\">Haga clic aqu\u00ed<\/a> to download the user guide.<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 5 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner-6\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Covaris M220 Focused-Ultrasonicator<\/a><\/div>\r\n\r\n<div id=\"collapseInner-6\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p><a href=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2025\/12\/M220.pdf\" target=\"_blank\">Haga clic aqu\u00ed<\/a> to download the user guide.<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 5 -->\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Group 1 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed\" href=\"#collapseTwo\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Full Service_Short read Library Preparation and Sequencing<\/a><\/div>\r\n<div id=\"collapseTwo\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p><strong>Library Construction:<\/strong> Core offers the following library construction as standard services<\/p>\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-1\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">TruSeq Stranded mRNA (PolyA+)<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-1\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide &gt;1 \u00b5g of total RNA with concentration between 50\u2013200 ng\/\u00b5l, RIN &gt; 8<\/p>\r\n\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC<\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project. <\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench.<\/li><\/ul> \r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 1 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-2\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">TruSeq Stranded Total RNA Gold <\/a><\/div>\r\n\r\n<div id=\"collapseInner2-2\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide >1ug of total RNA with concentration between 50-200ng\/ul, RIN>8.<\/p>\r\n\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li> Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop <\/li>concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench. <\/li><\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 2 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-3\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Ultra Low mRNA (SMART-Seq mRNA HT LP) <\/a><\/div>\r\n<div id=\"collapseInner2-3\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide 10pg-1ng of total RNA with concentration < 1ng\/uL, RIN>8.<\/p>\r\n\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC  <span style=\"color:#990000\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 3 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-4\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Illumina DNA Prep <\/a><\/div>\r\n\r\n<div id=\"collapseInner2-4\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide >100ng of DNA with concentration 5-50 ng\/ul with A260\/280=1.8-2.0, A260\/230=2.0-2.2<\/p>\r\n\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000;\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench. <\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 4 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-5\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">NEBNext Ultra II DNA (ChIP, Amplicon, <400bp) Prep (no fragmentation)<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-5\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide > 5 ng of DNA with concentration > 0.2 ng\/uL with A260\/280=1.8-2.0, A260\/230=2.0-2.2<\/p>\r\n\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000;\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 5 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-6\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">WatchMaker mRNA (PolyA+)<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-6\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide >500ng of total RNA with concentration between 50\u2013200 ng\/\u00b5l, RIN > 8<\/p>\r\n\r\n<ol>\r\n<li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC<\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a> under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10:00 a.m.-12:00 p.m. with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 6 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-7\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">WatchMaker total RNA<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-7\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p class=\"instruction\"><strong>Submission Instruction:<\/strong> Please provide >500ng of total RNA with concentration between 50\u2013200 ng\/\u00b5l, RIN > 8<\/p>\r\n\r\n<ol>\r\n<li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC<\/li>\r\n<li>Please submit stock samples in 8-well PCR strip tubes with individually attached caps. (USA Scientific. #1402-4700). <\/li>\r\n<li>Please save your completed form in the format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a> under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10:00 a.m.-12:00 p.m. with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 7 -->\r\n\r\n<p><strong>Sequencing:<\/strong> Core uses the following sequencers to address different coverages with different costs and turnaround time.<\/p>\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-8\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Novaseq X plus<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-8\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<table>\r\n  <caption>Flow Cell Types<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Flow Cell Type<\/th>\r\n      <th>Total Lanes (per flow cell)<\/th>\r\n      <th>Output (whole flowcell)<\/th>\r\n      <th>Kits options (per flow cell)<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">1.5B<\/td>\r\n      <td style=\"vertical-align: middle;\">2<\/td>\r\n      <td style=\"vertical-align: middle;\">1.5B<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        100 cycles<br>(max. 138 cycles)<br>\r\n        200 cycles<br>(max. 238 cycles)<br>\r\n        300 cycles<br>(max. 238 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">10B<\/td>\r\n      <td style=\"vertical-align: middle;\">8<\/td>\r\n      <td style=\"vertical-align: middle;\">10B<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        100 cycles<br>(max. 138 cycles)<br>\r\n        200 cycles<br>(max. 238 cycles)<br>\r\n        300 cycles<br>(max. 238 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">25B<\/td>\r\n      <td style=\"vertical-align: middle;\">8<\/td>\r\n      <td style=\"vertical-align: middle;\">25B<\/td>\r\n      <td style=\"vertical-align: middle;\">300 cycles<br>(max. 338 cycles)<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 8 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-9\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Nextseq 2000<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-9\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<table>\r\n  <caption>Nextseq 2000<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Flow Cell Type<\/th>\r\n      <th>Total Lanes (per flow cell)<\/th>\r\n      <th>Output (whole flowcell)<\/th>\r\n      <th>Kits options (per flow cell)<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">P1<\/td>\r\n      <td style=\"vertical-align: middle;\">1<\/td>\r\n      <td style=\"vertical-align: middle;\">100M<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        100 cycles kit<br>(max. 138 cycles)<br>\r\n        300 cycles kit<br>(max. 338 cycles)<br>\r\n        600 cycles kit<br>(max. 638 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">P2<\/td>\r\n      <td style=\"vertical-align: middle;\">1<\/td>\r\n      <td style=\"vertical-align: middle;\">400M<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        100 cycles kit<br>(max.138 cycles)<br>\r\n        200 cycles kit<br>(max.238 cycles)<br>\r\n        300 cycles kit<br>(max. 338 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">P3<\/td>\r\n      <td style=\"vertical-align: middle;\">1<\/td>\r\n      <td style=\"vertical-align: middle;\">1.1B<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        50 cycles kit<br>(max. 88 cycles)<br>\r\n        100 cycles kit<br>(max.138 cycles)<br>\r\n        200 cycles kit<br>(max.238 cycles)<br>\r\n        300 cycles kit<br>(max. 327 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 9 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-10\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Miniseq<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-10\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<table>\r\n  <caption>Miniseq<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Flow Cell Type<\/th>\r\n      <th>Total Lanes (per flow cell)<\/th>\r\n      <th>Output (whole flowcell)<\/th>\r\n      <th>Kits options (per flow cell)<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">HighOutput<\/td>\r\n      <td style=\"vertical-align: middle;\">1<\/td>\r\n      <td style=\"vertical-align: middle;\">22\u201325 M<\/td>\r\n      <td style=\"vertical-align: middle;\">\r\n        75 cycles kit<br>(max. 92 cycles)<br>\r\n        150 cycles kit<br>(max.168 cycles)<br>\r\n        300 cycles kit<br>(max.318 cycles)\r\n      <\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td style=\"vertical-align: middle;\">MidOutput<\/td>\r\n      <td style=\"vertical-align: middle;\">1<\/td>\r\n      <td style=\"vertical-align: middle;\">7\u20138 M<\/td>\r\n      <td style=\"vertical-align: middle;\">300 cycles kit<br>(max.318 cycles)<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 10 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner2-11\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Element Aviti<\/a><\/div>\r\n\r\n<div id=\"collapseInner2-11\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<table>\r\n  <caption>Element Aviti<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Flow Cell Type<\/th>\r\n      <th>Total Lanes (per flow cell)<\/th>\r\n      <th>Output (whole flowcell)<\/th>\r\n      <th>Kits options (per flow cell)<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td rowspan=\"3\" style=\"vertical-align: middle;\">High Output<\/td>\r\n      <td rowspan=\"3\" style=\"vertical-align: middle;\">2<\/td>\r\n      <td rowspan=\"2\" style=\"vertical-align: middle;\">1B<\/td>\r\n      <td style=\"vertical-align: middle;\">150 cycles kit<br>(max. 184 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n        <td style=\"vertical-align: middle;\">300 cycles kit<br>(max. 334 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n        <td style=\"vertical-align: middle;\">300M<\/td>\r\n        <td style=\"vertical-align: middle;\">600 cycles kit<br>(max. 634 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td rowspan=\"3\" style=\"vertical-align: middle;\">Medium Output<\/td>\r\n      <td rowspan=\"3\" style=\"vertical-align: middle;\">2<\/td>\r\n      <td rowspan=\"2\" style=\"vertical-align: middle;\">500M<\/td>\r\n      <td style=\"vertical-align: middle;\">150 cycles kit<br>max. 184 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n        <td style=\"vertical-align: middle;\">300 cycles kit<br>(max. 334 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n        <td style=\"vertical-align: middle;\">100M<\/td>\r\n        <td style=\"vertical-align: middle;\">600 cycles kit<br>(max. 634 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td rowspan=\"2\" style=\"vertical-align: middle;\">Low Output<\/td>\r\n      <td rowspan=\"2\" style=\"vertical-align: middle;\">2<\/td>\r\n      <td style=\"vertical-align: middle;\">250M<\/td>\r\n      <td style=\"vertical-align: middle;\">150 cycles kit<br>(max. 184 cycles)<\/td>\r\n    <\/tr>\r\n    <tr>\r\n        <td style=\"vertical-align: middle;\">100M<\/td>\r\n        <td style=\"vertical-align: middle;\">600 cycles kit<br>(max. 634 cycles)<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 11 -->\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Group 2 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed\" href=\"#collapseThree\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Full Service_Long Read PacBio Platform Library Preparation and Sequencing<\/a><\/div>\r\n\r\n<div id=\"collapseThree\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p><strong>Library Construction<\/strong>: Core offers the following library construction as standard services:\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner3-1\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Iso-Seq (full length transcript RNA-Seq)<\/a><\/div>\r\n<div id=\"collapseInner3-1\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n\r\n<p>Submission Instruction:<\/p>\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 1.5 mL Eppendorf tubes (low binding preferred) with sample code written on top of tube.<\/li> \r\n<li>Please follow this table to prepare your samples<\/li>\r\n\r\n<table>\r\n  <caption>Please refer to this table to prepare your samples<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Input<\/th>\r\n      <th>Total Input<\/th>\r\n      <th>Concentration<\/th>\r\n      <th>QC<\/th>\r\n      <th>Comentarios<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td>Total RNA<\/td>\r\n      <td>&gt; 300ng<\/td>\r\n      <td>50-200 ng\/\u00b5l<\/td>\r\n      <td>\r\n        A260\/280=1.8-2.0<br>\r\n        A260\/230=2.0-2.2<br>\r\n        RIN &gt; 7\r\n      <\/td>\r\n      <td>Resuspend in RNase-free water<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>cDNA<\/td>\r\n      <td>&gt; 1 \u00b5g<\/td>\r\n      <td><\/td>\r\n      <td>cDNA Profile (Tapestation or Bioanalyzer)<\/td>\r\n      <td>Resuspend in EB or low EDTA TE<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/li>\r\n<li>Please save the form in format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench. <\/li><\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 1 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner3-2\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Whole Genome Sequencing 15Kb HiFi <\/a><\/div>\r\n<div id=\"collapseInner3-2\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n\r\n<div class=\"accordion-inner\">\r\n<p>Submission Instruction:<\/p>\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 1.5 mL Eppendorf tubes (low binding preferred) with sample code written on top of tube.<\/li> \r\n<li>Please follow this table to prepare your samples<\/li>\r\n\r\n<table>\r\n  <caption>Please refer to this table to prepare your samples<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Input<\/th>\r\n      <th>Total Input<\/th>\r\n      <th>Concentration<\/th>\r\n      <th>QC<\/th>\r\n      <th>Comentarios<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td>HMW gDNA<\/td>\r\n      <td>&gt; 2\u00b5g<\/td>\r\n      <td>50-100 ng\/\u00b5l<\/td>\r\n      <td>\r\n        A260\/280=1.8-2.0<br>\r\nA260\/230=2.0-2.2           \r\n      <\/td>\r\n      <td>Resuspend in EB or low EDTA TE. <span style=\"color:#990000;\">**DNA should be at high quality and sufficient quantity since there is no amplification step.<\/span><\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/li>\r\n<li>Please save your completed form in format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 2 -->\r\n\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed sub\" href=\"#collapseInner3-3\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">PureTarget repeat expansion panel <\/a><\/div>\r\n\r\n<div id=\"collapseInner3-3\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p>Submission Instruction:<\/p>\r\n<ol><li>Download the form and follows the instructions to fill the form.<\/li>\r\n<li>Sample codes should be identical as the labels on the tube with your initials and sequential numbers. (Always start with 1 and do not skip number. i.e., NGS1, NGS2, NGS3, etc.)<\/li>\r\n<li>Sample Name can ONLY be named with alphabets, numbers, underscores, and not more than 20 characters respectively. (No space, +.,!@#$%^&#038; etc.)<\/li>\r\n<li>Please fill in Qubit and Tape station results on the form if available. Otherwise, please provide nanodrop concentration and submit 5 \u00b5l of samples in 8-well PCR strip tubes for QC <span style=\"color:#990000;\">(Don&#8217;t cut 8-strips and do not skip any well)<\/span><\/li>\r\n<li>Please submit stock samples in 1.5 mL Eppendorf tubes (low binding preferred) with sample code written on top of tube.<\/li> \r\n<li>Please follow this table to prepare your samples<\/li>\r\n\r\n<table>\r\n  <caption>Please refer to this table to prepare your samples<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Input<\/th>\r\n      <th>Total Input<\/th>\r\n      <th>Concentration<\/th>\r\n      <th>QC<\/th>\r\n      <th>Comentarios<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td>HMW gDNA<\/td>\r\n      <td>&gt; 2\u00b5g<\/td>\r\n      <td>50-100 ng\/\u00b5l<\/td>\r\n      <td>\r\n        A260\/280=1.8-2.0<br>\r\nA260\/230=2.0-2.2           \r\n      <\/td>\r\n      <td>EB, low EDTA TE (pH8)  <\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/li>\r\n<li>Please save your completed form in format of YYMMDD_PI_YourFirstName_LibrarySEQ_Form and upload it to <a href=\"https:\/\/ppms.us\/salk\/?IGC\">https:\/\/ppms.us\/salk\/?IGC<\/a>  under request\/start a new project<\/li>\r\n<li>Drop off samples Tuesday\/Thursday between 10am-12pm with dry ice and sign the drop off sheet on the drop off bench.<\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Subgroup 3 -->\r\n\r\n<p><strong>Sequencing:<\/strong> We use the lasted technology Revio for fast turnaround time and getting the reliable data.\r\n<table>\r\n  <caption>HiFi Sequencing Performance<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Library<\/th>\r\n      <th>Run time*<\/th>\r\n      <th>Q30+ bases<\/th>\r\n      <th>HiFi yield per SMRT Cell<\/th>\r\n      <th>Methylation<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td>1\u20135 kb<\/td>\r\n      <td>12 hours<\/td>\r\n      <td>95%<\/td>\r\n      <td>6\u20138 million reads<\/td>\r\n      <td><\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>5\u201310 kb<\/td>\r\n      <!-- If run time and Q30+ are unknown, leave blank or fill in accordingly -->\r\n      <td><\/td>\r\n      <td><\/td>\r\n      <td>35\u201370 Gb<\/td>\r\n      <td><\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>10\u201315 kb<\/td>\r\n      <td>24 hours<\/td>\r\n      <td>90%<\/td>\r\n      <td>70\u2013100 Gb<\/td>\r\n      <td><\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>15\u201320 kb<\/td>\r\n      <td><\/td>\r\n      <td><\/td>\r\n      <td>100\u2013120 Gb<\/td>\r\n      <td><\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>20\u201325 kb<\/td>\r\n      <td>30 hours<\/td>\r\n      <td>85%<\/td>\r\n      <td>100\u2013120 Gb<\/td>\r\n      <td>5mC at CpG sites and 6mA<sup>\u2020<\/sup> for native DNA<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n\r\n<!-- Second Table: Key applications and sample throughput with SPRQ chemistry -->\r\n<table>\r\n  <caption>Key Applications and Sample Throughput with SPRQ Chemistry<\/caption>\r\n  <thead>\r\n    <tr>\r\n      <th>Library<\/th>\r\n      <th>Aplicaci\u00f3n<\/th>\r\n      <th>Per Revio SMRT Cell<\/th>\r\n      <th>Expected coverage<\/th>\r\n    <\/tr>\r\n  <\/thead>\r\n  <tbody>\r\n    <tr>\r\n      <td>1\u20135 kb<\/td>\r\n      <td>Amplicon sequencing<\/td>\r\n      <td>&gt;1,000<\/td>\r\n      <td>50\u00d7<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>5\u201310 kb<\/td>\r\n      <td>Microbial genome<\/td>\r\n      <td>384<\/td>\r\n      <td>30\u00d7<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>5\u201310 kb<\/td>\r\n      <td>PureTarget repeat expansion panel<\/td>\r\n      <td>48<\/td>\r\n      <td>200\u00d7<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>15\u201320 kb<\/td>\r\n      <td>Human genome<\/td>\r\n      <td>2<\/td>\r\n      <td>20\u00d7<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>15\u201320 kb<\/td>\r\n      <td>Human methylation profiling<\/td>\r\n      <td>8<\/td>\r\n      <td>5\u00d7<\/td>\r\n    <\/tr>\r\n    <tr>\r\n      <td>15\u201320 kb<\/td>\r\n      <td>Transcriptome with Kinnex&#x2122; full-length RNA kit<\/td>\r\n      <td>6<\/td>\r\n      <td>10 million reads<\/td>\r\n    <\/tr>\r\n  <\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Group 3 -->\r\n\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed\" href=\"#collapseFour\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Full Service_Long Read NanoPore Platform Library Preparation and Sequencing<\/a><\/div>\r\n<div id=\"collapseFour\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n\r\nMore information coming soon.\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<!-- End Group 4 -->\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>","protected":false},"excerpt":{"rendered":"<style>\r\n  table {\r\n    border-collapse: collapse;\r\n    width: 100%;\r\n    font-family: Arial, sans-serif;\r\n    border: 1px solid #000;\r\n  }\r\n\r\n  th, td {\r\n    border: 1px solid #000;\r\n    padding: 8px;\r\n    vertical-align: bottom;\r\n  }\r\n\r\n  thead th {\r\n    background: #002060; \/* Dark Blue *\/\r\n    color: #fff; \/* White text *\/\r\n    font-weight: bold;\r\n    text-align: left;\r\n  }\r\n\r\n  tbody td {\r\n    background: #B8CCE4; \/* Light Blue body *\/\r\n    color: #000; \/* Dark text *\/\r\n  }\r\n\r\n.instruction {\r\n  background-color: #fff8e1; \/* a soft yellow pastel *\/\r\n  padding: 1em;\r\n  border-radius: 4px;\r\n  border: 1px solid #ffe0b2;\r\n  font-weight: bold;\r\n}\r\n<\/style>\r\n<a class=\"openall btn -expand\">Expand All<\/a><a class=\"closeall btn -expand\">Collapse All<\/a>\r\n<div id=\"accordion2\" class=\"accordion\" style=\"margin-top: 20px;\">\r\n<div class=\"accordion-group\">\r\n<div class=\"accordion-heading\"><a class=\"accordion-toggle collapsed\" href=\"#collapseOne\" data-toggle=\"collapse\" data-parent=\"#accordion2\" aria-expanded=\"false\">Self Service_Shared Instruments<\/a><\/div>\r\n<div id=\"collapseOne\" class=\"accordion-body collapse\" style=\"height: 0px;\" aria-expanded=\"false\">\r\n<div class=\"accordion-inner\">\r\n<p>Core Provide training for all shared instruments, and they are operated under Self Service.\r\nPlease use this link <a href=\"https:\/\/ppms.us\/salk\/listsys\/?pf=9\">https:\/\/ppms.us\/salk\/listsys\/?pf=9<\/a> to book those instruments on PPMS \r\n<\/p>","protected":false},"author":3,"featured_media":0,"parent":526,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-core-facilities-services.php","meta":{"_acf_changed":false,"footnotes":""},"folder":[542],"class_list":["post-51960","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Instrumentation &amp; 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Please use this link https:\/\/ppms.us\/salk\/listsys\/?pf=9 to book those instruments on PPMS\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/instrumentation-and-services\/\" \/>\n<meta property=\"og:site_name\" content=\"Salk Institute for Biological Studies\" \/>\n<meta property=\"article:modified_time\" content=\"2025-12-01T19:48:40+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk-institute-preview-image.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"628\" \/>\n\t<meta property=\"og:image:height\" content=\"329\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"12 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/instrumentation-and-services\\\/\",\"url\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/instrumentation-and-services\\\/\",\"name\":\"Instrumentation & Services - Salk Institute for Biological Studies\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#website\"},\"datePublished\":\"2024-12-18T05:24:15+00:00\",\"dateModified\":\"2025-12-01T19:48:40+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/instrumentation-and-services\\\/#breadcrumb\"},\"inLanguage\":\"es-MX\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/instrumentation-and-services\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/instrumentation-and-services\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.salk.edu\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Science\",\"item\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Core Facilities\",\"item\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"The Razavi Newman Integrative Genomics and Bioinformatics Core\",\"item\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/integrative-genomics-and-bioinformatics-core\\\/\"},{\"@type\":\"ListItem\",\"position\":5,\"name\":\"Instrumentation &#038; Services\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#website\",\"url\":\"https:\\\/\\\/www.salk.edu\\\/\",\"name\":\"Salk Institute for Biological Studies\",\"description\":\"The Power of Science\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.salk.edu\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"es-MX\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#organization\",\"name\":\"Salk Institute for Biological Studies\",\"url\":\"https:\\\/\\\/www.salk.edu\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"es-MX\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"http:\\\/\\\/www.salk.edu\\\/wp-content\\\/uploads\\\/2023\\\/03\\\/salk_logo_696.jpg\",\"contentUrl\":\"http:\\\/\\\/www.salk.edu\\\/wp-content\\\/uploads\\\/2023\\\/03\\\/salk_logo_696.jpg\",\"width\":696,\"height\":696,\"caption\":\"Salk Institute for Biological Studies\"},\"image\":{\"@id\":\"https:\\\/\\\/www.salk.edu\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Instrumentation & Services - Salk Institute for Biological Studies","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/instrumentation-and-services\/","og_locale":"es_MX","og_type":"article","og_title":"Instrumentation & Services - Salk Institute for Biological Studies","og_description":"Expand AllCollapse All   Self Service_Shared Instruments   Core Provide training for all shared instruments, and they are operated under Self Service. 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Services"}]},{"@type":"WebSite","@id":"https:\/\/www.salk.edu\/#website","url":"https:\/\/www.salk.edu\/","name":"Instituto Salk de Estudios Biol\u00f3gicos","description":"The Power of Science","publisher":{"@id":"https:\/\/www.salk.edu\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.salk.edu\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"es-MX"},{"@type":"Organization","@id":"https:\/\/www.salk.edu\/#organization","name":"Instituto Salk de Estudios Biol\u00f3gicos","url":"https:\/\/www.salk.edu\/","logo":{"@type":"ImageObject","inLanguage":"es-MX","@id":"https:\/\/www.salk.edu\/#\/schema\/logo\/image\/","url":"http:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk_logo_696.jpg","contentUrl":"http:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk_logo_696.jpg","width":696,"height":696,"caption":"Salk Institute for Biological Studies"},"image":{"@id":"https:\/\/www.salk.edu\/#\/schema\/logo\/image\/"}}]}},"ACF":{"hero":"https:\/\/www.salk.edu\/wp-content\/uploads\/2015\/09\/02A14N40-1920.jpg","line_1":"The Razavi Newman<br\/>Integrative Genomics and Bioinformatics Core","line_2":"Instrumentation and Services","gallery":false,"secondary_navigation":"<div class=\"menu-integrative-genomics-and-bioinformatics-core-container\"><ul id=\"menu-integrative-genomics-and-bioinformatics-core\" class=\"menu\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5401\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/\">Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5400\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/people\/\">People<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-52734\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/instrumentation-and-services\/\">Instrumentation &#038; Services<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-52737\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/rates\/\">Scheduling &#038; Rates<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5396\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/integrative-genomics-and-bioinformatics-core\/contact\/\">Contact<\/a><\/li>\n<\/ul><\/div>","salk_custom_body_class":"","poster_quote":""},"_links":{"self":[{"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/pages\/51960","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/comments?post=51960"}],"version-history":[{"count":52,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/pages\/51960\/revisions"}],"predecessor-version":[{"id":55356,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/pages\/51960\/revisions\/55356"}],"up":[{"embeddable":true,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/pages\/526"}],"wp:attachment":[{"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/media?parent=51960"}],"wp:term":[{"taxonomy":"folder","embeddable":true,"href":"https:\/\/www.salk.edu\/es\/wp-json\/wp\/v2\/folder?post=51960"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}