{"id":5190,"date":"2015-10-20T17:47:29","date_gmt":"2015-10-21T00:47:29","guid":{"rendered":"https:\/\/vermont.salk.edu\/?page_id=5190"},"modified":"2025-11-03T13:15:43","modified_gmt":"2025-11-03T21:15:43","slug":"scheduling-and-rates","status":"publish","type":"page","link":"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/","title":{"rendered":"Horarios y tarifas"},"content":{"rendered":"

For ordering information and request submission please email GT3@salk.edu<\/a>.\r\n\r\n<\/p>

Rates are effective as of 07\/01\/25 and are subject to change. Labs located in California will be charged California sales tax in addition to the prices listed.<\/strong><\/p>\r\n\r\n\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n
LENTIVIRAL AND RETROVIRAL VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n <\/thead>\r\n
Custom Lentiviral\/Retroviral Prep<\/td>\r\n $1,455<\/td>\r\n $2,383<\/td>\r\n \r\n <\/tr>\r\n
Stock Lentiviral Aliquot (5ul)<\/td>\r\n $73<\/td>\r\n $120<\/td>\r\n \r\n <\/tr>\r\n
Stock Retroviral Aliquot (5ul)<\/td>\r\n $73<\/td>\r\n $120<\/td>\r\n \r\n <\/tr>\r\n
AAV VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n\r\n \r\n
Custom AAV Prep, Large Scale<\/td>\r\n $3,380<\/td>\r\n $5,537<\/td>\r\n \r\n <\/tr>\r\n
Custom AAV Prep, Small Scale<\/td>\r\n $1,557<\/td>\r\n $2,550<\/td>\r\n \r\n <\/tr>\r\n
Stock AAV Aliquot (25ul)<\/td>\r\n $48<\/td>\r\n $78<\/td>\r\n \r\n <\/tr>\r\n\r\n
G-DELETED RABIES VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Custom G-Deleted Rabies Prep from GT3 Seed Vial<\/td>\r\n $9,539<\/td>\r\n $15,624<\/td>\r\n \r\n <\/tr>\r\n
Stock G-Deleted Rabies Aliquot (5ul)<\/td>\r\n $94<\/td>\r\n $154<\/td>\r\n \r\n <\/tr>\r\n
AD5 VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Stock ADV Aliquot (50 ul)<\/td>\r\n $238<\/td>\r\n $390<\/td>\r\n \r\n <\/tr>\r\n
VSV VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Stock VSV Aliquot (5ul)<\/td>\r\n $94<\/td>\r\n $154<\/td>\r\n \r\n <\/tr>\r\n
HSV VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Stock HSV Aliquot (5ul)<\/td>\r\n $164<\/td>\r\n $268<\/td>\r\n \r\n <\/tr>\r\n
LCMV VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Custom LCMV Prep<\/td>\r\n $3,278<\/td>\r\n $5,370<\/td>\r\n \r\n <\/tr>\r\n
Stock LCMV Aliquot (3 ml)<\/td>\r\n $41<\/td>\r\n $67<\/td>\r\n \r\n <\/tr>\r\n
EIAV VECTORS<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Custom EIAV Prep<\/td>\r\n $1,455<\/td>\r\n $2,383<\/td>\r\n \r\n <\/tr>\r\n
Stock EIAV Aliquot (5ul)<\/td>\r\n $73<\/td>\r\n $120<\/td>\r\n \r\n <\/tr>\r\n
Miscellaneous Services<\/th>\r\n C3 Member<\/th>\r\n External Non-Profit<\/th>\r\n \r\n <\/tr>\r\n
Cell Line (1 ml)<\/td>\r\n $128<\/td>\r\n $209<\/td>\r\n \r\n <\/tr>\r\n <\/table>\t\t\r\n
","protected":false},"excerpt":{"rendered":"

For ordering information and request submission please email GT3@salk.edu<\/a>.\r\n\r\n<\/p>","protected":false},"author":3,"featured_media":0,"parent":525,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-general-page.php","meta":{"_acf_changed":false,"footnotes":""},"folder":[522],"class_list":["post-5190","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nScheduling & Rates - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Scheduling & Rates - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"For ordering information and request submission please email GT3@salk.edu.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/\" \/>\n<meta property=\"og:site_name\" content=\"Salk Institute for Biological Studies\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-03T21:15:43+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk-institute-preview-image.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"628\" \/>\n\t<meta property=\"og:image:height\" content=\"329\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/viral-vector-core\\\/scheduling-and-rates\\\/\",\"url\":\"https:\\\/\\\/www.salk.edu\\\/science\\\/core-facilities\\\/viral-vector-core\\\/scheduling-and-rates\\\/\",\"name\":\"Scheduling & Rates - 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Salk Institute for Biological Studies","isPartOf":{"@id":"https:\/\/www.salk.edu\/#website"},"datePublished":"2015-10-21T00:47:29+00:00","dateModified":"2025-11-03T21:15:43+00:00","breadcrumb":{"@id":"https:\/\/www.salk.edu\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/#breadcrumb"},"inLanguage":"es-MX","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.salk.edu\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.salk.edu\/science\/core-facilities\/viral-vector-core\/scheduling-and-rates\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.salk.edu\/"},{"@type":"ListItem","position":2,"name":"Science","item":"https:\/\/www.salk.edu\/science\/"},{"@type":"ListItem","position":3,"name":"Core Facilities","item":"https:\/\/www.salk.edu\/science\/core-facilities\/"},{"@type":"ListItem","position":4,"name":"Gene Transfer, Targeting and Therapeutics Viral Vector Core","item":"https:\/\/www.salk.edu\/science\/core-facilities\/viral-vector-core\/"},{"@type":"ListItem","position":5,"name":"Scheduling & Rates"}]},{"@type":"WebSite","@id":"https:\/\/www.salk.edu\/#website","url":"https:\/\/www.salk.edu\/","name":"Instituto Salk de Estudios Biol\u00f3gicos","description":"The Power of Science","publisher":{"@id":"https:\/\/www.salk.edu\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.salk.edu\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"es-MX"},{"@type":"Organization","@id":"https:\/\/www.salk.edu\/#organization","name":"Instituto Salk de Estudios Biol\u00f3gicos","url":"https:\/\/www.salk.edu\/","logo":{"@type":"ImageObject","inLanguage":"es-MX","@id":"https:\/\/www.salk.edu\/#\/schema\/logo\/image\/","url":"http:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk_logo_696.jpg","contentUrl":"http:\/\/www.salk.edu\/wp-content\/uploads\/2023\/03\/salk_logo_696.jpg","width":696,"height":696,"caption":"Salk Institute for Biological Studies"},"image":{"@id":"https:\/\/www.salk.edu\/#\/schema\/logo\/image\/"}}]}},"ACF":{"hero":"https:\/\/www.salk.edu\/wp-content\/uploads\/2015\/09\/07_Crop002_ProjMax001_final.jpg","line_1":"Gene Transfer, Targeting and Therapeutics<br>Viral Vector Core","line_2":"Scheduling & Rates","gallery":false,"rates":"<h2>Forms<\/h2>\n<p>Order from available pre-made GT3 Stocks:<\/p>\n<ul class=\"indent\">\n<li>Click here to download a request form for aliquots of viral vector reporter stocks<\/li>\n<\/ul>\n<p>Order a custom vector production using your transfer construct or one of the transfer constructs available at the core.<\/p>\n<ul class=\"indent\">\n<li>Click here to see the list of current list of AAV, FIV, LV, Retro or EIAV transfer plasmids available at no charge at the core<\/li>\n<li> Click here to download a request form for custom recombinant viral vector production<\/li>\n<\/ul>\n<h2>Recharge Rates<\/h2>\n<p><\/p>\n<p><a href=\"http:\/\/in.salk.edu\/?page_id=7632\">Click to visit the Salk Intranet for internal pricing>><\/a>\n<\/p>\n<p>The Salk GT<sup>3<\/sup> core provides concentrated high titer stocks of pseudotyped lentivirus (HIV-1 and EIAV) and retrovirus (MMLV), E1-deleted Adenovirus (Ad5) and rAAV2 cross-packaged with a range of different capsids.\n<\/p>\n<p>\nRates are given for standard preparations from 12 x 15 cm dishes, which typically yield ~200 ul 10<sup>8<\/sup>-10<sup>10<\/sup> GC\/ml lentivirus or ~3.5 ml of 10<sup>11<\/sup>-10<sup>13<\/sup> GC\/ml rAAV. Purity is suitable for both <em>in vitro<\/em> and <em>in vivo<\/em> applications.<\/p>\n<ul class=\"indent\">\n<li><a href=\"#bbb\">Lentiviral and Retroviral Vectors<\/a><\/li>\n<li><a href=\"#ccc\">rAAV2 vectors<\/a><\/li>\n<li><a href=\"#ddd\">G-deleted rabies<\/a><\/li>\n<li><a href=\"#eee\">Ad5 vectors<\/a><\/li>\n<\/ul>\n<p><\/p>\n<h2><a name=\"bbb\" id=\"bbb\"><\/a>Lentiviral and Retroviral<br \/>\nVectors<\/h2>\n<p>Large-scale production and purification of pseudotyped lentiviral and retroviral vectors is performed by transient transfection of 12 x 15 cm dishes followed by ultracentrifugation on a sucrose cushion. Upon request, the sucrose cushion can be replaced with purification using large volume of PSB, which was found to lower the viscosity of the preps. <\/p>\n<p>\nMedium containing replication-deficient lentiviral or retroviral particles is harvested twice and pooled before filtration and concentration. Concentrated virus is resuspended in HBSS, aliquoted and stored at -80 degrees C. <\/p>\n<p>\nCustom preps are titrated by serial dilution and transduction of 293T cells. Titer is obtained by qPCR (no reporter) to give infectious titer in integrated genome copies (IU) per mL or, upon request, by FACS (if fluorescent reporter present) to give titer in transforming units (TU) per mL.<\/p>\n","data":[{"title":"","table":"<table>\r\n<tbody>\r\n<tr>\r\n<td><\/td>\r\n<td><strong>C3\/SCRM Member<\/strong><\/td>\r\n<td><strong>External<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td><strong>Consultation<\/strong><\/td>\r\n<td>$50 per hr<\/td>\r\n\r\n<td>$50 per hr<\/td>\r\n<\/tr>\r\n<tr>\r\n\r\n<td><strong>Standard Prep with titration<\/strong><\/td>\r\n<td>$1200<\/td>\r\n<td>$2000<\/td>\r\n<\/tr>\r\n\r\n<tr>\r\n<td><strong>Stock reporter\r\n virus (10 uL)<\/strong><\/td>\r\n<td>$80<\/td>\r\n<td>$140<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","description":"<p><a href=\"http:\/\/in.salk.edu\/?page_id=7632\">Click to visit the Salk Intranet for internal pricing>><\/a><\/p>\n<p><br \/><\/p>\n<h2><a name=\"ccc\" id=\"ccc\"><\/a>rAAV2 vectors<\/h2>\n<p>\nStandard scale production and purification of cross-packaged rAAV vector is performed by transient transfection of 12 x 15 cm dishes followed by purification on a discontinous OptiprepTM gradient. Further polishing by size exclusion is available by request.<\/p>\n<p>\nLarge scale production and purification is performed by transient transfection of 60 x 15 cm dishes followed by purification on two consecutive CsCl gradients. Collected fraction are analysis by separation on SDS gel and silver staining, RI determination and QPCR analysis. The fractions contained highest percentage of full capsids and lowest amount of contaminating proteins are pulled and dialyzed against PBS and PBS\/Sorbitol. <\/p>\n<p>\nGradient fractions containing purified rAAV infectious particles are aliquoted and stored at -80 degrees C.<\/p>\n<p>\nCustom rAAV preps are titrated using qPCR to give titer in genome copies (GC) per ml.<\/p>"},{"title":"","table":"<table>\r\n<tbody>\r\n<tr>\r\n<td><\/td>\r\n<td><strong>C3\/SCRM Member<\/strong><\/td>\r\n<td><strong>External<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td><strong>Consultation<\/strong><\/td>\r\n\r\n<td>$50\r\n per hr<\/td>\r\n<td>$50\r\n per hr<\/td>\r\n<\/tr>\r\n\r\n\r\n<tr>\r\n<td><strong>Standard Prep with\r\n titration (Iodoxanol)<\/strong><\/td>\r\n<td>$1200<\/td>\r\n<td>$2000<\/td>\r\n<\/tr>\r\n<tr>\r\n <td><strong>Iodoxanol Stock reporter vector (50ul)<\/strong><\/td>\r\n <td>$80<\/td>\r\n <td>$130<\/td>\r\n<\/tr>\r\n\r\n\r\n<tr>\r\n <td><strong>Large Prep with titration (CsCl)<\/strong><\/td>\r\n <td>$1700<\/td>\r\n <td>$2900<\/td>\r\n<\/tr>\r\n<tr>\r\n <td><strong>CsCl Stock reporter vector (50ul)<\/strong><\/td>\r\n <td>$160<\/td>\r\n <td>$270<\/td>\r\n<\/tr>\r\n\r\n<\/tbody>\r\n<\/table>\r\n","description":"<p><a href=\"http:\/\/in.salk.edu\/?page_id=7632\">Click to visit the Salk Intranet for internal pricing>><\/a><\/p>\n<p><br \/><\/p>\n<h2><a name=\"ddd\" id=\"ddd\"><\/a>G-deleted rabies<\/h2>\n<p><\/p>\n<p>Large<br \/>\n scale production and purification of G-deleted rabies vectors is performed on BHK21 cells and the vector is amplified folowing initial infection with seed virus.<\/p>\n<p> Purified G-deleted rabies infectious particles are aliquoted and stored at <nobr>-80 degrees C.<\/nobr><\/p>\n<p> Custom rabies vector preps are titrated using infection of reporter cells and qPCR to give titer in infectious units(IU)<br \/>\n per ml.<\/p>"},{"title":"","table":"<table>\r\n <tbody>\r\n <tr>\r\n <td><\/td>\r\n <td><strong>C3\/SCRM Member<\/strong><\/td>\r\n\r\n <td><strong>External<\/strong><\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Consultation<\/strong><\/td>\r\n <td>$50\r\n per hr<\/td>\r\n <td>$50\r\n per hr<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Standard Prep from seed with\r\n titration<\/strong><\/td>\r\n <td>$2400<\/td>\r\n <td>$4000<\/td>\r\n\r\n <\/tr>\r\n \r\n <tr>\r\n <td><strong>Stock G-deleted rabies (10ul)<\/strong><\/td>\r\n <td>$130<\/td>\r\n <td>$200<\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Custom Rabies Recovery from DNA<\/strong><\/td>\r\n <td>$3500<\/td>\r\n <td>$5850<\/td>\r\n <\/tr>\r\n\r\n <\/tbody>\r\n<\/table>","description":"<p><br \/><\/p>\n<p><a href=\"http:\/\/in.salk.edu\/?page_id=7632\">Click to visit the Salk Intranet for internal pricing>><\/a><br \/>\n<\/p>\n<p><br \/><\/p>\n<h2><a name=\"eee\" id=\"eee\"><\/a>Ad5 vectors<\/h2>\n<p>Large<br \/>\n scale production and purification of E1a\/b and E3 deleted Ad5 vectors is by<br \/>\n transient transfection of Pac 1 digested Ad5 vector DNA, amplification of vector on 293 cells followed by purification on<br \/>\n a discontinous Optiprep<sup>TM<\/sup> gradient and polishing using a size exclusion column.<\/p>\n<p> Gradient fractions containing purified Ad5 infectious particles are aliquoted and stored at <nobr>-80 degrees C.<\/nobr><\/p>\n<p> Custom Ad5 preps are titrated using qPCR to give titer in genome<br \/>\n copies (GC)<br \/>\n per ml.<\/p>"},{"title":"","table":"<table>\r\n <tbody>\r\n <tr>\r\n <td><\/td>\r\n <td><strong>C3\/SCRM Member<\/strong><\/td>\r\n\r\n <td><strong>External<\/strong><\/td>\r\n <\/tr>\r\n <tr>\r\n <td><strong>Consultation<\/strong><\/td>\r\n <td>$50\r\n per hr<\/td>\r\n <td>$50\r\n per hr<\/td>\r\n <\/tr>\r\n \r\n <tr>\r\n <td><strong>Standard Prep with\r\n titration (from stock)<\/strong><\/td>\r\n <td>$1700<\/td>\r\n <td>$2800<\/td>\r\n\r\n <\/tr>\r\n <tr>\r\n <td><strong>Standard Prep with\r\n titration (from DNA)<\/strong><\/td>\r\n <td>$2300<\/td>\r\n <td>$3900<\/td>\r\n <\/tr>\r\n \r\n <tr>\r\n <td><strong>Ad5 stocks<\/strong><\/td>\r\n <td>$100<\/td>\r\n <td>$160<\/td>\r\n <\/tr>\r\n\r\n <\/tbody>\r\n<\/table>\r\n","description":"<p><br \/><\/p>\n<p><a href=\"http:\/\/in.salk.edu\/?page_id=7632\">Click to visit the Salk Intranet for internal pricing>><\/a><\/p>"}],"secondary_navigation":"<div class=\"menu-gene-transfer-targeting-and-therapeutics-core-container\"><ul id=\"menu-gene-transfer-targeting-and-therapeutics-core\" class=\"menu\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5195\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/\">Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5194\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/people\/\">People<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5193\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/instrumentation-services\/\">Instrumentation & Services<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-42449\"><a href=\"https:\/\/www.salk.edu\/es\/science\/core-facilities\/viral-vector-core\/rates\/\">Scheduling & Rates<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-5524\"><a 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