{"id":17477,"date":"2018-04-19T12:44:27","date_gmt":"2018-04-19T19:44:27","guid":{"rendered":"https:\/\/vermont.salk.edu\/?page_id=17477"},"modified":"2024-01-30T14:04:18","modified_gmt":"2024-01-30T22:04:18","slug":"cores","status":"publish","type":"page","link":"https:\/\/www.salk.edu\/es\/science\/research-centers\/salk-cancer-center\/cores\/","title":{"rendered":"Recursos compartidos y n\u00facleos"},"content":{"rendered":"
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N\u00facleo de Biofot\u00f3nica Avanzada<\/a><\/h2>\n

El N\u00facleo Avanzado de Biofot\u00f3nica proporciona instrumentaci\u00f3n de imagen y an\u00e1lisis junto con personal t\u00e9cnico y de apoyo colaborativo para microscop\u00eda avanzada de luz y electr\u00f3nica de sistemas biol\u00f3gicos. Los miembros del Centro de C\u00e1ncer utilizan la instalaci\u00f3n para ensayos de imagen de alto rendimiento, imagen de alta resoluci\u00f3n de la din\u00e1mica de c\u00e9lulas vivas y tejidos, microscop\u00eda de superresoluci\u00f3n, imagen de grandes vol\u00famenes 3D de tejidos, an\u00e1lisis de microscop\u00eda electr\u00f3nica de la morfolog\u00eda subcelular y distribuci\u00f3n de prote\u00ednas, y procesamiento, visualizaci\u00f3n y an\u00e1lisis computacional automatizado de im\u00e1genes. El N\u00facleo tambi\u00e9n est\u00e1 persiguiendo y desarrollando activamente nuevas metodolog\u00edas de vanguardia en imagen y an\u00e1lisis para servir mejor a las necesidades de los investigadores del Centro de C\u00e1ncer, como la microscop\u00eda correlativa de luz y electr\u00f3nica criog\u00e9nica, la imagen de hoja de luz de tejidos aclarados y expandidos, y el procesamiento, segmentaci\u00f3n y an\u00e1lisis basado en aprendizaje autom\u00e1tico de im\u00e1genes de microscop\u00eda de luz y electr\u00f3nica.<\/p>\n

Leer m\u00e1s<\/a><\/p>\n<\/div>\n<\/div>\n

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Citometr\u00eda de Flujo Central<\/a><\/h2>\n

El Laboratorio de Citometr\u00eda de Flujo ofrece a los investigadores de Salk acceso a instrumentaci\u00f3n de citometr\u00eda de flujo de vanguardia, servicios de alta calidad y consulta experta en el dise\u00f1o e implementaci\u00f3n de experimentos basados en citometr\u00eda de flujo. La instalaci\u00f3n est\u00e1 equipada con dos clasificadores de c\u00e9lulas activados por fluorescencia (FACS) operados por personal y tres instrumentos anal\u00edticos que est\u00e1n disponibles para operaci\u00f3n independiente. En el Laboratorio se ofrece capacitaci\u00f3n y apoyo a usuarios de todos los niveles de experiencia. El personal de la instalaci\u00f3n fomenta un ambiente colaborativo, esforz\u00e1ndose por proporcionar un recurso integral de f\u00e1cil acceso a los investigadores del Centro de C\u00e1ncer y dedica esfuerzos para identificar tecnolog\u00edas o aplicaciones de citometr\u00eda de flujo emergentes que ser\u00e1n \u00fatiles para los usuarios del Laboratorio.<\/p>\n

Leer m\u00e1s<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n

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Secuenciaci\u00f3n de Nueva Generaci\u00f3n N\u00facleo<\/a><\/h2>\n

La misi\u00f3n del N\u00facleo de Secuenciaci\u00f3n de Nueva Generaci\u00f3n es facilitar la investigaci\u00f3n gen\u00f3mica de vanguardia. En apoyo de los esfuerzos de investigaci\u00f3n del Centro de C\u00e1ncer en el Salk, el N\u00facleo tiene como objetivo espec\u00edfico proporcionar: 1) servicios de secuenciaci\u00f3n de alto rendimiento r\u00e1pidos y rentables, 2) asistencia experta en el dise\u00f1o experimental, 3) el desarrollo de m\u00e9todos novedosos para permitir tecnolog\u00edas de secuenciaci\u00f3n de vanguardia, 4) la preparaci\u00f3n y el control de calidad de las bibliotecas de secuenciaci\u00f3n, y 5) servicios de capacitaci\u00f3n para miembros del Centro de C\u00e1ncer en preparaci\u00f3n de bibliotecas u otros m\u00e9todos requeridos para proyectos de secuenciaci\u00f3n de nueva generaci\u00f3n.<\/p>\n

Leer m\u00e1s<\/a><\/p>\n<\/div>\n<\/div>\n

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Transferencia G\u00e9nica, Dirigido y Terap\u00e9utica N\u00facleo de Vectores Virales<\/a><\/h2>\n

Los vectores virales se usan com\u00fanmente para entregar material gen\u00e9tico a c\u00e9lulas espec\u00edficas, tanto en in vitro e in vivo<\/em>. Tambi\u00e9n representan candidatos atractivos para in vivo<\/em> Terapia g\u00e9nica. Sin embargo, existen consideraciones de seguridad significativas al trabajar y producir vectores virales de grado de investigaci\u00f3n. Estos problemas de seguridad se ven agravados cuando los laboratorios de investigaci\u00f3n individuales tienen la tarea de establecer y mantener su propia infraestructura y protocolos de seguridad. Para abordar estas preocupaciones, el Instituto Salk ha establecido un recurso centralizado, a saber, el N\u00facleo de Vectores Virales (Viral Vector Core). El N\u00facleo produce y mantiene una extensa biblioteca de vectores virales de stock de uso com\u00fan. Adem\u00e1s, el N\u00facleo ofrece servicios de dise\u00f1o y producci\u00f3n personalizados para m\u00faltiples tipos de vectores virales, incluidos lentivirus, retrovirus, adenovirus, virus adenoasociado, rabia con eliminaci\u00f3n de genes G y virus de la estomatitis vesicular.<\/p>\n

Leer m\u00e1s<\/a> Recursos Compartidos NCI C3<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n

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N\u00facleo de Gen\u00f3mica Integrativa y Bioinform\u00e1tica<\/a><\/h2>\n

El N\u00facleo de Gen\u00f3mica Integrativa y Bioinform\u00e1tica proporciona servicios anal\u00edticos, computacionales y t\u00e9cnicos para todos los aspectos de la investigaci\u00f3n en gen\u00f3mica y bioinform\u00e1tica que realizan los miembros del Centro de C\u00e1ncer de Salk. En la era de los macrodatos en biolog\u00eda, el an\u00e1lisis bioinform\u00e1tico es crucial para obtener informaci\u00f3n biol\u00f3gica a partir de datos complejos de alto rendimiento y con ruido. El objetivo del N\u00facleo ha sido brindar a los miembros del Centro de C\u00e1ncer acceso a experiencia y apoyo de vanguardia en bioinform\u00e1tica. El personal del N\u00facleo asiste a los miembros del Centro de C\u00e1ncer con el an\u00e1lisis integrativo de conjuntos de datos de secuenciaci\u00f3n, proporciona software y herramientas que los miembros del Centro de C\u00e1ncer pueden usar para realizar sus propios an\u00e1lisis, brinda capacitaci\u00f3n en el uso de software y desarrolla herramientas y flujos de trabajo personalizados para an\u00e1lisis de vanguardia.<\/p>\n

Leer m\u00e1s<\/a><\/p>\n<\/div>\n<\/div>\n

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Espectrometr\u00eda de Masas Centralizada: Prote\u00f3mica y Metabol\u00f3mica<\/a><\/h2>\n

El Centro de Espectrometr\u00eda de Masas ofrece a los miembros del Centro de C\u00e1ncer apoyo para sus necesidades de prote\u00f3mica, metabol\u00f3mica y s\u00edntesis de p\u00e9ptidos a medida. El Centro ofrece consultor\u00eda experta sobre el dise\u00f1o, implementaci\u00f3n y an\u00e1lisis de una amplia gama de experimentos, incluyendo: identificaci\u00f3n de prote\u00ednas, cuantificaci\u00f3n de prote\u00ednas, identificaci\u00f3n de modificaciones post-traduccionales, an\u00e1lisis de prote\u00ednas\/p\u00e9ptidos secretados, perfilado de lipid\u00f3mica no dirigida, lipid\u00f3mica y metabol\u00f3mica dirigida, an\u00e1lisis de \u00e1cidos biliares, cuantificaci\u00f3n de amino\u00e1cidos, an\u00e1lisis de \u00e1cidos grasos y s\u00edntesis de p\u00e9ptidos a medida. El personal del Centro trabaja constantemente para implementar nuevas t\u00e9cnicas anal\u00edticas en estos campos en r\u00e1pida evoluci\u00f3n, al tiempo que apoya a los investigadores en el an\u00e1lisis de datos y la preparaci\u00f3n de manuscritos y subvenciones.<\/p>\n

Leer m\u00e1s<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n

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N\u00facleo Transg\u00e9nico<\/a><\/h2>\n

El objetivo principal del N\u00facleo Transg\u00e9nico es proporcionar a los miembros del Centro de C\u00e1ncer y a otros investigadores del Instituto Salk acceso a tecnolog\u00edas de vanguardia para crear modelos de ratones gen\u00e9ticamente alterados. Estas l\u00edneas de ratones transg\u00e9nicos representan herramientas gen\u00e9ticas cr\u00edticas que permiten a los investigadores modelar enfermedades humanas, analizar y manipular funcionalmente genes espec\u00edficos, y probar posibles intervenciones terap\u00e9uticas. Para lograr este objetivo, el N\u00facleo Transg\u00e9nico proporciona servicios de edici\u00f3n gen\u00f3mica de \u00faltima generaci\u00f3n, as\u00ed como los servicios necesarios para generar y mantener ratones transg\u00e9nicos.<\/p>\n

Leer m\u00e1s<\/a> Recursos Compartidos NCI C3<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>","protected":false},"excerpt":{"rendered":"

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N\u00facleo de Biofot\u00f3nica Avanzada<\/a><\/h2>\n

El N\u00facleo Avanzado de Biofot\u00f3nica proporciona instrumentaci\u00f3n de imagen y an\u00e1lisis junto con personal t\u00e9cnico y de apoyo colaborativo para microscop\u00eda avanzada de luz y electr\u00f3nica de sistemas biol\u00f3gicos. Los miembros del Centro de C\u00e1ncer utilizan la instalaci\u00f3n para ensayos de imagen de alto rendimiento, imagen de alta resoluci\u00f3n de la din\u00e1mica de c\u00e9lulas vivas y tejidos, microscop\u00eda de superresoluci\u00f3n, imagen de grandes vol\u00famenes 3D de tejidos, an\u00e1lisis de microscop\u00eda electr\u00f3nica de la morfolog\u00eda subcelular y distribuci\u00f3n de prote\u00ednas, y procesamiento, visualizaci\u00f3n y an\u00e1lisis computacional automatizado de im\u00e1genes. El N\u00facleo tambi\u00e9n est\u00e1 persiguiendo y desarrollando activamente nuevas metodolog\u00edas de vanguardia en imagen y an\u00e1lisis para servir mejor a las necesidades de los investigadores del Centro de C\u00e1ncer, como la microscop\u00eda correlativa de luz y electr\u00f3nica criog\u00e9nica, la imagen de hoja de luz de tejidos aclarados y expandidos, y el procesamiento, segmentaci\u00f3n y an\u00e1lisis basado en aprendizaje autom\u00e1tico de im\u00e1genes de microscop\u00eda de luz y electr\u00f3nica.<\/p>","protected":false},"author":91,"featured_media":0,"parent":17510,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-scc-shared-cores.php","meta":{"_acf_changed":false,"footnotes":""},"folder":[551],"class_list":["post-17477","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nShared Resources & Cores - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/es\/science\/research-centers\/salk-cancer-center\/cores\/\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Shared Resources & Cores - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"Advanced Biophotonics Core The Advanced Biophotonics Core provides imaging and analysis instrumentation coupled with technical and collaborative support staff for advanced light and electron microscopy of biological systems. 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