{"id":2115,"date":"2011-07-19T00:00:00","date_gmt":"2011-07-19T07:00:00","guid":{"rendered":"https:\/\/vermont.salk.edu\/news-release\/salk-researchers-develop-method-to-map-cell-receptor-that-regulates-stress\/"},"modified":"2011-07-19T00:00:00","modified_gmt":"2011-07-19T07:00:00","slug":"salk-researchers-develop-method-to-map-cell-receptor-that-regulates-stress","status":"publish","type":"disclosure","link":"https:\/\/www.salk.edu\/es\/news-release\/salk-researchers-develop-method-to-map-cell-receptor-that-regulates-stress\/","title":{"rendered":"Salk researchers develop method to map cell receptor that regulates stress"},"content":{"rendered":"

LA JOLLA, CA\u2014Drug developers have long been looking for agents that will target a cell receptor that regulates stress in humans, but no small molecule drugs have successfully gone through clinical studies. Now, a team at the Salk Institute has demonstrated how a novel tool can be used to map the binding sites on this receptor, which they say could speed the design of effective therapies.\n<\/p>\n

\nIn the July 12th online international issue of Angewandte Chemie<\/em>, the researchers published a method demonstrating how unnatural amino acids (Uaas) can be used to map the structure of a corticotropin-releasing hormone receptor (CRF-R1). They also show how this new tool helped locate three areas on the receptor to which peptide hormones can dock to activate or inhibit the receptor. Many other so-called natural ligand binding sites are predicted to exist on the receptor.\n<\/p>\n

\"non-natural<\/p>\n

A non-natural amino acid is inserted in the GPCR, expressed at the cell membrane, to capture ligands.<\/p>\n

Photo: Courtesy of Dr. Irene Coin, Salk Institute for Biological Studies<\/p>\n<\/div>\n

\n“As important as this receptor is to regulating human stress, no one knows how it is exactly arranged in the cell membrane, which is key to understanding how it functions and how it can be manipulated in drug therapy,” says the study’s first author, Dr. Irene Coin, Ph.D. “We have created a method that provides new insights into how peptides interact with this receptor.”\n<\/p>\n

\nNot only is this method useful to understanding the specific function of CRF-R1, it can be adapted to map the interaction of other G-protein-coupled receptors (GPCRs), a large family of proteins of which CRF-R1 is a member, says the study’s senior author, Dr. Lei Wang<\/a>, assistant professor and Frederick B. Rentschler Developmental Chair in the Chemical Biology and Proteomics Laboratory.\n<\/p>\n

\n“There are hundreds of GPCRs in the human body, and they are the molecular target of more than 30% of today’s pharmaceuticals,” says Wang. “This technology has enormous potential for addressing fundamental biological questions regarding the many different classes of GPCRs, and for guiding the design of exquisitely specific agents where they are needed.”\n<\/p>\n

\nThe study evolved from several fundamental Salk discoveries. The first is the 1981 discovery and cloning of the CRF receptor by Salk researcher Dr. Wylie Vale<\/a>, who is a co-author on this study. The receptor binds on to the coricotropin-releasing hormone, which mediates endocrine, autonomic, behavioral, and immune responses to stress.\n<\/p>\n

\nThe second innovation is the development of Uaas technology by Wang and his colleagues. It was first used in bacteria in 2001 and then in mammalian cells in 2007. This study is the first to report its application in comparing the behavior of different ligands at the receptor in live mammalian cells.\n<\/p>\n

\nCoin, who was trained in Germany, joined Wang’s laboratory as a postdoctoral fellow to lead the study. “This is one of the few labs in the world that can do this kind of research on mammalian cells,” she says. “And in Vale’s lab there is unbeatable expertise in the biological testing of the mutant receptors.”\n<\/p>\n

\nThe tool the researchers use consists of three different molecules, all of which are synthetically engineered. One is a synthetic transfer RNA (tRNA) that cells use to incorporate amino acids into a protein that is being built inside a cell. The second molecule is an enzymatic synthetase that can recognize the engineered tRNA, and load it with the third engineered molecule – an unnatural amino acid. These amino acids are chemically distinct from the 20 amino acids that naturally exist in the body; they can be engineered for different desirable properties, such as to fluoresce or to chemically react upon exposure to light.\n<\/p>\n

\nBecause the researchers know the amino acid sequences of the CRF-R1, they modified the DNA at specific sites that they believed might be near ligand binding sites. The engineered tRNA and synthetase worked together to implant an unnatural amino acid at these sites. “We can control the positions that this unnatural amino acid end up in, and we put them where we think there might be a binding site, based on some clues from other data,” says Coin. After adding radiolabeled natural ligands to cells expressing the receptor, the researchers then shine light, which triggers the unnatural amino acid to capture the ligand if the latter is close by. “We can now tell if the ligand binds close to a particular site of the receptor or not,” she says.\n<\/p>\n

\nIn this way, different mutants displaying an unnatural amino acid in a different structural position on the receptor can be engineered. “This is a way to map the surface of the receptor to see which positions are close to ligands,” Coin says.\n<\/p>\n

\nDifferent ligands may bind in different places on the receptor, so the technology can be used to eventually build a complete map of all the ligand-specific binding sites on the receptor, Wang says. With that knowledge, drugs can eventually be shaped that specifically adhere to the targeted binding pocket, he says.\n<\/p>\n

\nThe researchers screened 12 different positions on the receptor, and found three natural ligand binding sites. “We published the methodology and provided the first preliminary map of binding sites,” Coin says. “Different ligands indeed show different binding patterns.”\n<\/p>\n

\nDr. Marilyn Perrin, a staff scientist at Salk’s Clayton Foundation Laboratories for Peptide Biology, also contributed to the research.\n<\/p>\n

\nThe study was funded by a postdoctoral fellowship provided to Coin from the Deutsche Forschungsgemeinschaft, and grants from the National Institute of Diabetes and Digestive and Kidney Disease, the Clayton Medical Research Foundation, the California Institute for Regenerative Medicine, and the National Institutes of Health.\n<\/p>\n


\nAcerca del Instituto Salk de Estudios Biol\u00f3gicos:<\/strong>
\nEl Instituto Salk de Estudios Biol\u00f3gicos es una de las instituciones de investigaci\u00f3n b\u00e1sica m\u00e1s destacadas del mundo, donde un cuerpo docente de prestigio internacional investiga cuestiones fundamentales de las ciencias de la vida en un entorno \u00fanico, colaborativo y creativo. Centrados tanto en el descubrimiento como en la formaci\u00f3n de las futuras generaciones de investigadores, los cient\u00edficos del Salk realizan contribuciones revolucionarias a nuestra comprensi\u00f3n del c\u00e1ncer, el envejecimiento, el Alzheimer, la diabetes y las enfermedades infecciosas mediante el estudio de la neurociencia, la gen\u00e9tica, la biolog\u00eda celular y vegetal, y otras disciplinas relacionadas.\n<\/p>\n

\nLos logros del cuerpo docente han sido reconocidos con numerosos galardones, entre los que se incluyen premios Nobel y la pertenencia a la Academia Nacional de Ciencias. Fundado en 1960 por el Dr. Jonas Salk, pionero en la vacuna contra la poliomielitis, el Instituto es una organizaci\u00f3n independiente sin fines de lucro y un hito arquitect\u00f3nico.<\/p>","protected":false},"featured_media":0,"template":"","faculty":[],"disease-research":[],"class_list":["post-2115","disclosure","type-disclosure","status-publish","hentry"],"acf":[],"yoast_head":"\nSalk researchers develop method to map cell receptor that regulates stress - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/es\/news-release\/salk-researchers-develop-method-to-map-cell-receptor-that-regulates-stress\/\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Salk researchers develop method to map cell receptor that regulates stress - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"LA JOLLA, CA\u2014Drug developers have long been looking for agents that will target a cell receptor that regulates stress in humans, but no small molecule drugs have successfully gone through clinical studies. 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