{"id":10437,"date":"2016-07-14T09:53:09","date_gmt":"2016-07-14T16:53:09","guid":{"rendered":"https:\/\/vermont.salk.edu\/?post_type=disclosure&p=10437"},"modified":"2024-01-30T15:20:06","modified_gmt":"2024-01-30T23:20:06","slug":"gauging-stem-cells-for-regenerative-medicine","status":"publish","type":"disclosure","link":"https:\/\/www.salk.edu\/es\/news-release\/gauging-stem-cells-for-regenerative-medicine\/","title":{"rendered":"Gauging stem cells for regenerative medicine"},"content":{"rendered":"

LA JOLLA\u2014Salk scientists and colleagues have proposed new molecular criteria for judging just how close any line of laboratory-generated stem cells comes to mimicking embryonic cells seen in the very earliest stages of human development, known as na\u00efve stem cells. The tests found that no current protocols lead to truly na\u00efve stem cells, but the guidelines may help researchers achieve that goal by pointing out where each current method falls short. Generating na\u00efve stem cells would be a boon to both basic research and to medical applications of stem cells, such as growing tissue for organ replacement.<\/p>\n

\"Naive-WIN1-5iLA-Primed-WIBR3\"
Salk researchers and collaborators provide new benchmark for generating the most primitive type of stem cell. Visualized above is a naive human ESC line (WIN1, pictured left) and, for comparison, a primed human ESC line (WIBR3). Both of these lines were used in the study.<\/p>\n

Haga clic aqu\u00ed<\/a> for a high-resolution image<\/p>\n

Cr\u00e9dito: Instituto Salk<\/figcaption><\/figure>\n

\u201cThe na\u00efve state potentially has a greater ability to generate different tissue types and could have many uses for regenerative medicine<\/a>,\u201d says senior author Joseph Ecker<\/a>, professor and director of Salk’s Genomic Analysis Laboratory and a Howard Hughes Medical Institute<\/a> investigator. The work was published online July 14, 2016 in Cell Stem Cell<\/a><\/em>.<\/p>\n

While stem cells\u2014cells that have the potential to differentiate into other types of cells\u2014exist in adult humans, the most useful stem cells are those found in embryos, which are pluripotent, capable of becoming nearly any cell in the body. Researchers have developed cocktails of molecules that turn back the clock on adult cells to make them act as stem cells (called induced pluripotent stem cells or iPSCs), and also have cultured lines of stem cells derived directly from embryos (ESCs). New methods are being formulated to coax the \u201cprimed\u201d ESCs\u2014which more resemble cells from post-implantation embryos\u2014back in time even more to resemble na\u00efve stem cells, those found in pre-implantation embryos only days after fertilization. Na\u00efve stem cells are blank slates that form the basis for not only all the cells of the human body, but cells that make up the placenta to support an embryo as well.<\/p>\n

\u201cIn our opinion, most of the published protocols to generate so-called na\u00efve stem cells are not convincing because they produce cells that are very much like the starting cells\u2014there\u2019s not much difference in gene expression,\u201d says co-senior author Rudolf Jaenisch<\/a> of the Whitehead Institute for Biomedical Research<\/a> y Massachusetts Institute of Technology<\/a>.<\/p>\n

Ecker, working closely with Jaenisch and other collaborators at Whitehead and the Ecole Polytechnique Federale de Lausanne<\/a>, wanted to see whether these new techniques aiming to induce the na\u00efve stem cell state truly did that. They performed a series of molecular tests on the \u201cprimed\u201d cells and ESCs that had been exposed to factors thought to induce na\u00efvety. They estimated the states of the two ESCs by comparing their molecular property and the cells from various stages in embryonic development from previous studies.<\/p>\n

Three main tests, they found, were most indicative of the difference between na\u00efve stem cells and other stem cells, letting them place each line of ESCs correctly along this timeline. First, they measured the expression levels of transposons, DNA sequences that can jump around the genome. The expression of certain transposons, they discovered, was indicative of na\u00efve stem cells. Next, they found that the genomes of na\u00efve embryonic stem cells have less methylation\u2014the addition of methyl chemical groups along DNA. They then studied the state of X chromosomes in na\u00efve cells of female embryos\u2019, which each contain two active X chromosomes, unlike more mature embryonic cells that have silenced one X.<\/p>\n

\"Joe
From left: Joseph Ecker, Zhuzhu Zhang, Ryan O\u2019Neil and Yupeng He<\/p>\n

Haga clic aqu\u00ed<\/a> for a high-resolution image<\/p>\n

Cr\u00e9dito: Instituto Salk<\/figcaption><\/figure>\n

Together, the three tests include tens of thousands of genetic biomarkers to characterize the developmental state of stem cells, says co-senior author Didier Trono<\/a> of the Ecole Polytechnique Federale de Lausanne. \u201cThis type of analysis is likely to become a gold standard for quality controlling stem cells, including induced pluripotent stem cells, whether they are to be used solely in research or envisioned for clinical applications,\u201d he says.<\/p>\n

When current methods for generating na\u00efve stem cells in the lab were judged using the three tests, each fell short of mimicking the na\u00efve embryonic cells in different ways. One new technique, for instance, led to cells that had two active X chromosomes but didn\u2019t match the exact methylation patterns desired.<\/p>\n

\u201cThis really was a comparison of existing methods, applying the same criteria to each method and seeing where each cell state is,\u201d says Ecker. \u201cSome of these cells ended up being in states earlier in development and some later in development.\u201d<\/p>\n

Thorold Theunissen, a postdoctoral fellow in Jaenisch\u2019s lab and co-first author of the study, says \u201cOur work provides a rigorous set of criteria for comparing na\u00efve human stem cells to their counterparts in the early human embryo. Previous studies mainly relied on comparisons with mouse stem cells, which are highly divergent from human.\u201d<\/p>\n

The scientists hope that other research teams adopt their criteria to judge their own methods and cell lines. \u201cThe transposon and methylation profiles are pretty standard in terms of technique and protocol so it\u2019s pretty easy for other labs to repeat the experiment on, for example, na\u00efve cells from a newly developed method,\u201d says Yupeng He, a graduate student in the Ecker lab who helped lead the work.<\/p>\n

Other researchers on the study were Styliani Markoulaki, Haoyi Wang, Malkiel A. Cohen, Katherine J. Wert, Yanmei Huang, Jesse Drotar, and Tenzin Lungjangwa of the Whitehead Institute for Biomedical Research; Marc Friedli, Evarist Planet, Julien Pontis, Alexandra Iouranova, Michael Imbeault, and Julien Duc of the Ecole Polytechnique Federale de Lausanne; and Ryan C. O\u2019Neil, Rosa Castanon, Zhuzhu Zhang, and Joseph R. Nery of the Salk Institute.<\/p>\n

The work and the researchers were supported by grants from the Fundaci\u00f3n Simons<\/a>, Institutos Nacionales de Salud<\/a>, Swiss National Science Foundation<\/a>, European Research Council<\/a>, Howard Hughes Medical Institute<\/a>, Gordon and Betty Moore Foundation<\/a>, Mary K. Chapman Foundation<\/a>, a Sir Henry Wellcome Postdoctoral Fellowship<\/a>, a Foundation Bettencourt<\/a> Award, the Association Pour La Recherche Sur Le Cancer<\/a>, y el Fonds de la Recherche en Sante du Quebec<\/a>.<\/p>","protected":false},"featured_media":10433,"template":"","faculty":[42],"disease-research":[146],"class_list":["post-10437","disclosure","type-disclosure","status-publish","has-post-thumbnail","hentry","faculty-joseph-ecker","disease-research-aging-and-regenerative-medicine"],"acf":[],"yoast_head":"\nGauging stem cells for regenerative medicine - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/es\/news-release\/gauging-stem-cells-for-regenerative-medicine\/\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gauging stem cells for regenerative medicine - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"LA JOLLA\u2014Salk scientists and colleagues have proposed new molecular criteria for judging just how close any line of laboratory-generated stem cells comes to mimicking embryonic cells seen in the very earliest stages of human development, known as na\u00efve stem cells. 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