{"id":5686,"date":"2015-10-27T16:40:45","date_gmt":"2015-10-27T23:40:45","guid":{"rendered":"https:\/\/vermont.salk.edu\/?page_id=5686"},"modified":"2026-01-30T11:56:28","modified_gmt":"2026-01-30T19:56:28","slug":"resources","status":"publish","type":"page","link":"https:\/\/www.salk.edu\/de\/science\/core-facilities\/cell-technologies-and-engineering-core\/resources\/","title":{"rendered":"Instrumentation &#038; Services"},"content":{"rendered":"<p>The Cell Technologies and Engineering Core houses 3 cell culture suites, 2 lab benches, and cell analysis. Each cell culture suite is outfitted with 3-4 bio-safety cabinets, 6-8 CO2 incubators, a low oxygen incubator, and inverted microscopes and requisite equipment for stem cell culture. The CTEC is located adjacent to the <a href=\"https:\/\/www.salk.edu\/de\/science\/core-facilities\/flow-cytometry\/\">Flow Cytometry Core Facility<\/a> enabling efficient cell sorting and cytometry analysis experiments.<\/p>\n<h2>Instrumentation<\/h2>\n<p><strong>Cell Imaging<\/strong><br \/>\n2x Dissection microscopes for manual isolation and manipulation of stem cell colonies<br \/>\nSeaHorse 96XFe for cell analysis<br \/>\n2x Discover Echo Cellcyte live cell imagers housed within CO<sub>2<\/sub> incubators<br \/>\nMaxwell Multielectrode array system for neuron activity analysis<\/p>\n<p><strong>Cell Culture<\/strong><br \/>\n11x Biological safety cabinets<br \/>\n18x C0<sub>2<\/sub> Incubators<br \/>\n3x N2\/C0<sub>2<\/sub> Incubators (hypoxic conditions)<br \/>\n3x Bench top centrifuges<br \/>\n3x 37\u00b0C Water baths<\/p>\n<p><strong>Transfection<\/strong><br \/>\nLifeTech Neon \u2013 Tip based electroporation (adjustable parameters)<br \/>\nMaxCyte ExPERT A+X electroporator (Cuvette-based; fixed parameter)<\/p>\n<p><strong>Centrifugation<\/strong><br \/>\nOptima L-80XP Ultracentrifuge<br \/>\nAllegra X-15R Plate centrifuge<br \/>\nBench top temperature controlled centrifuge &amp; microcentrifuge<\/p>\n<p><strong>Refrigerators and Freezers<\/strong><br \/>\n3x -80\u00b0C freezers<br \/>\n10x -20\u00b0C freezers<br \/>\n6x 4\u00b0C refrigerators<br \/>\n2x High capacity deli refrigerators<br \/>\n2x MVE 800 Series Cyrostorage freezers &#8211; 11664 vial capacity, auto fill controllers<br \/>\n2x LABS40K High-Capacity Cryostorage freezers &#8211; 31590 vial capacity, auto fill controllers<\/p>\n<h2 style=\"margin-top: 40px;\">Dienstleistungen<\/h2>\n<p>Biopsy processing (fibroblast derevation)<br \/>\nReprogramming from fibs or PBMCs<br \/>\nCustom genome editing<br \/>\niPSC QC (by request)<\/p>\n<ul>\n<li>Karyotyping<\/li>\n<li>Mycoplasma testing<\/li>\n<li>STR<\/li>\n<li>Undifferentiated state panel<\/li>\n<li>TriLineage differentiation<\/li>\n<li>Sendai virus genome retention assays<\/li>\n<\/ul>\n<p>Fib QC (by request)<\/p>\n<ul>\n<li>Mycoplasma<\/li>\n<li>Growth rate<\/li>\n<li>STR profiling (human only)<\/li>\n<\/ul>\n<p>Genome editing QC (by request)<\/p>\n<ul>\n<li>Mycoplasma<\/li>\n<li>Allele sequencing<\/li>\n<li>STR<\/li>\n<\/ul>\n<p>Mouse model development services<\/p>\n<ul>\n<li>CRISPR design<\/li>\n<li>CRISPR genotyping<\/li>\n<li>Custom cloning\/vector design and construction<\/li>\n<li>De novo ES cell Derivation<\/li>\n<li>DR4 and CF1 mEF cells<\/li>\n<li>ES cell expansion<\/li>\n<li>Gene targeting<\/li>\n<\/ul>\n<p><strong>Access:<\/strong><br \/>\nThe Cell Technologies and Engineering Core Facility is available to researchers 7 days a week, 24 hours a day. Access to the facility must be arranged in advance and approved by the CTEC.<\/p>\n<p style=\"margin-bottom: 0;\"><strong>Conditions of Use:<\/strong><\/p>\n<ol>\n<li>The Core Director must first approve all new cell lines that will be brought into the facility, including both live and frozen cell cultures, from both commercial and academic sources. All new, approved cell lines will be cultured under quarantine conditions until they have tested clean for mycoplasma in 2 consecutive tests.<\/li>\n<li>All new CTEC users must be approved by, and go through a brief orientation with the Core Director, or Lab Manager.<\/li>\n<li>Do not bring previously opened reagents, supplies or materials from other labs into the facility. In some instances this may be necessary, but must first be cleared with the Core Director. Unopened, direct from supplier, cell culture media and materials may be brought into the facility.<\/li>\n<\/ol>\n<p style=\"margin-top: 20px; margin-bottom: 0;\"><strong>SCRO approvals, ethics training, and MTAs:<\/strong><br \/>\nAny research involving human pluripotent stem cells at the Salk Institute will require:<\/p>\n<ol>\n<li>an SCRO approved protocol\u2028and<\/li>\n<li>ethics training for the researchers and PI involved with the experiments.<\/li>\n<\/ol>\n<p style=\"margin-top: 20px;\">Please consult the <a href=\"https:\/\/salkland.salk.edu\/Interact\/Pages\/Section\/Default.aspx?Section=4340\" target=\"_blank\" rel=\"noopener\">SCRO Website<\/a> for information on fulfilling these requirements. The requirements for approval will vary depending on the research project. The Core Director and staff can assist new researchers with the process of filling SCRO protocols. For training purposes, new investigators can begin working under the existing stem cell culture techniques training protocol. However, as the investigator(s) develop their own research project, separate SCRO approval must be obtained.<\/p>\n<p>Information on ethics training can be found online <a href=\"https:\/\/salkland.salk.edu\/interact\/Pages\/Content\/Document.aspx?id=3246\" target=\"_blank\" rel=\"noopener\">here\u00bb<\/a><\/p>\n<p>Salk researchers working in the CTEC are covered under the existing MTAs for the cell lines provided. Should an investigator want to culture one of the cell lines below in their own lab, a separate MTA would need to be executed.<\/p>\n<h2 style=\"margin-top: 40px;\">Cell Lines Available<\/h2>\n<p>All cell lines are karyotypically stable, pathogen free, and cultured without antibiotics. Additional cell lines available upon request.<\/p>\n<p style=\"margin-bottom: 0;\"><strong>Human Embryonic Stem Cell Lines Available:<\/strong><\/p>\n<table class=\"gridtable\" width=\"100%\">\n<tbody>\n<tr>\n<th>Cell Line<\/th>\n<th>Passage # on MEFS<\/th>\n<th>Passage # on mtg\/TeSR<\/th>\n<\/tr>\n<tr>\n<td>H1<\/td>\n<td>p32<\/td>\n<td>p39<\/td>\n<\/tr>\n<tr>\n<td width=\"150\">H7<\/td>\n<td>p26<\/td>\n<td>p28<\/td>\n<\/tr>\n<tr>\n<td>H9<\/td>\n<td>p33<\/td>\n<td>p35<\/td>\n<\/tr>\n<tr>\n<td>H13<\/td>\n<td>p30<\/td>\n<td>p32<\/td>\n<\/tr>\n<tr>\n<td>HUES-3<\/td>\n<td>p29<\/td>\n<td>p30<\/td>\n<\/tr>\n<tr>\n<td>HUES-6<\/td>\n<td>p21<\/td>\n<td>p24<\/td>\n<\/tr>\n<tr>\n<td>HUES-8<\/td>\n<td>p32<\/td>\n<td>&#8211;<\/td>\n<\/tr>\n<tr>\n<td>HUES-9<\/td>\n<td>&#8211;<\/td>\n<td>p33<\/td>\n<\/tr>\n<tr>\n<td>LSJ-1<\/td>\n<td>&#8211;<\/td>\n<td>p24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Human Induced Pluripotent Stem Cell Lines Available:<\/strong><br \/>\nDisease Specific iPSCs: Are you looking for a specific disease-linked iPSC line? It might already exist and we might be able to help you find it.<\/p>\n<table class=\"gridtable\" width=\"100%\">\n<tbody>\n<tr>\n<th>Cell Line<\/th>\n<th>Passage # on MEFS<\/th>\n<th>Passage # on mtg\/TeSR<\/th>\n<\/tr>\n<tr>\n<td width=\"150\">BJ-iPSC<\/td>\n<td>p10-28<\/td>\n<td>p10-12<\/td>\n<\/tr>\n<tr>\n<td>WiCell-iPSC<\/td>\n<td>&#8211;<\/td>\n<td>p33<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Other cell types available:<\/strong><br \/>\nKeratinocytes-Adult<br \/>\nKeratinocytes-Neo Natal<br \/>\nBJ Fibroblasts<br \/>\nIMR90s<br \/>\nOP9s<br \/>\n293Ts<br \/>\nHFFs<br \/>\nMEFs<br \/>\nHUVEC<\/p>\n<p><strong>MTA Considerations<\/strong><br \/>\nSalk researchers working in the CTEC are covered under the existing MTAs for the cell lines provided. Should an investigator want to culture one of these cell lines in their own lab, a separate MTA would need to be executed.<\/p>","protected":false},"excerpt":{"rendered":"<p>The Cell Technologies and Engineering Core houses 3 cell culture suites, 2 lab benches, and cell analysis. Each cell culture suite is outfitted with 3-4 bio-safety cabinets, 6-8 CO2 incubators, a low oxygen incubator, and inverted microscopes and requisite equipment for stem cell culture. The CTEC is located adjacent to the <a href=\"https:\/\/www.salk.edu\/de\/science\/core-facilities\/flow-cytometry\/\">Flow Cytometry Core Facility<\/a> enabling efficient cell sorting and cytometry analysis experiments.<\/p>","protected":false},"author":3,"featured_media":0,"parent":531,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-core-facilities-resources.php","meta":{"_acf_changed":false,"footnotes":""},"folder":[529],"class_list":["post-5686","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Instrumentation &amp; Services - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/de\/science\/core-facilities\/cell-technologies-and-engineering-core\/resources\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Instrumentation &amp; Services - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"The Cell Technologies and Engineering Core houses 3 cell culture suites, 2 lab benches, and cell analysis. 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