{"id":47269,"date":"2024-02-08T01:45:48","date_gmt":"2024-02-08T09:45:48","guid":{"rendered":"https:\/\/vermont.salk.edu\/?post_type=disclosure&p=47269"},"modified":"2024-02-08T09:05:20","modified_gmt":"2024-02-08T17:05:20","slug":"faulty-dna-disposal-system-causes-inflammation","status":"publish","type":"disclosure","link":"https:\/\/www.salk.edu\/de\/news-release\/faulty-dna-disposal-system-causes-inflammation\/","title":{"rendered":"Faulty DNA disposal system causes inflammation"},"content":{"rendered":"

LA JOLLA\u2014Cells in the human body contain power-generating mitochondria, each with their own mtDNA\u2014a unique set of genetic instructions entirely separate from the cell\u2019s nuclear DNA that mitochondria use to create life-giving energy. When mtDNA remains where it belongs (inside of mitochondria), it sustains both mitochondrial and cellular health\u2014but when it goes where it doesn\u2019t belong, it can initiate an immune response that promotes inflammation.\r\n<\/p>\r\n

\"Gerald<\/a>
From left: Gerald Shadel, Laura Newman, and Uri Manor.
\r\nKlicken Sie hier<\/a> f\u00fcr ein hochaufl\u00f6sendes Bild.
Kredit: Salk Institut<\/figcaption><\/figure>

\r\nNow, Salk scientists and collaborators at UC San Diego have discovered a novel mechanism used to remove improperly functioning mtDNA from inside to outside the mitochondria. When this happens, the mtDNA gets flagged as foreign DNA and activates a cellular pathway normally used to promote inflammation to rid the cell of pathogens, like viruses. \r\n<\/p>\r\n

\r\nThe findings, published in Nature Cell Biology<\/em><\/a> on February 8, 2024, offer many new targets for therapeutics to disrupt the inflammatory pathway and therefore mitigate inflammation during aging and diseases, like lupus or rheumatoid arthritis.\r\n<\/p>

\r\n\u201cWe knew that mtDNA was escaping mitochondria, but how was still unclear,\u201d says senior and co-corresponding author Professor Gerald Shadel<\/a>, director of the San Diego-Nathan Shock Center of Excellence in the Basic Biology of Aging and holder of the Audrey Geisel Chair in Biomedical Science at Salk. \u201cUsing imaging and cell biology approaches, we\u2019re able to trace the steps of the pathway for moving mtDNA out of the mitochondria, which we can now try to target with therapeutic interventions to hopefully prevent the resulting inflammation.\u201d\r\n<\/p>\r\n

\r\nOne of the ways our cells respond to damage and infection is with what\u2019s known as the innate immune system. While the innate immune response is the first line of defense against viruses, it can also respond to molecules the body makes that simply resemble pathogens\u2014including misplaced mtDNA. This response can lead to chronic inflammation and contribute to human diseases and aging. \r\n<\/p>\r\n

\"Endosomes<\/a>
Endosomes (magenta) collect around mitochondria (blue) after infection with virus HSV-1, which attacks mtDNA (green) and causes its release.
\r\nKlicken Sie hier<\/a> f\u00fcr ein hochaufl\u00f6sendes Bild.
Kredit: Salk Institut<\/figcaption><\/figure>\r\n

\r\nScientists have been working to uncover how mtDNA leaves mitochondria and triggers the innate immune response, but the previously characterized pathways did not apply to the unique mtDNA stress conditions the Salk team was investigating. So, they turned to sophisticated imaging techniques to gather clues as to where and when things were going awry in those mitochondria.\r\n<\/p>\r\n

\"We had a huge breakthrough when we saw that mtDNA was inside of a mysterious membrane structure once it left mitochondria\u2014after assembling all of the puzzle pieces, we realized that structure was an endosome,\u201d says first author Laura Newman<\/a>, former postdoctoral researcher in Shadel\u2019s lab and current assistant professor at the University of Virginia. \u201cThat discovery eventually led us to the realization that the mtDNA was being disposed of and, in the process, some of it was leaking out.\u201d\r\n<\/p>

\r\nThe team discovered a process beginning with a malfunction in mtDNA replication that caused mtDNA-containing protein masses called nucleoids to pile up inside of mitochondria. Noticing this malfunction, the cell then begins to remove the replication-halting nucleoids by transporting them to endosomes, a collection of organelles that sort and send cellular material for permanent removal. The endosome gets overloaded with these nucleoids, springs a leak, and mtDNA is suddenly loose in the cell. The cell flags that mtDNA as foreign DNA\u2014the same way it flags a virus\u2019s DNA\u2014and initiates the DNA-sensing cGAS-STING pathway to cause inflammation.\r\n<\/p>

\r\n\u201cUsing our cutting-edge imaging tools for probing mitochondria dynamics and mtDNA release, we have discovered an entirely novel release mechanism for mtDNA,\u201d says co-corresponding author Uri Manor, former director of the Waitt Advanced Biophotonics Core at Salk and current assistant professor at UC San Diego. \u201cThere are so many follow-up questions we cannot wait to ask, like how other interactions between organelles control innate immune pathways, how different cell types release mtDNA, and how we can target this new pathway to reduce inflammation during disease and aging.\u201d\r\n<\/p>

\r\nThe researchers hope to map out more of this complicated mtDNA-disposal and immune-activation pathway, including what biological circumstances\u2014like mtDNA replication dysfunction and viral infection\u2014are required to initiate the pathway and what downstream effects there may be on human health. They also see an opportunity for therapeutic innovation using this pathway, which represents a new cellular target to reduce inflammation. \r\n<\/p>

\r\nOther authors include Sammy Weiser Novak, Gladys Rojas, Nimesha Tadepalle, Cara Schiavon, Christina Towers, Matthew Donnelly, Sagnika Ghosh, Sienna Rocha, and Ricardo Rodriguez-Enriquez of Salk; Danielle Grotjahn and Michaela Medina of The Scripps Research Institute; Marie-\u00c8ve Tremblay of the University of Victoria in Canada; Joshua Chevez of UC San Diego; and Ian Lemersal of the La Jolla Institute for Immunology.\r\n<\/p>

\r\nThe work was supported by the National Institutes of Health (R01 AR069876, P30AG068635, 1K99GM141482, 1F32GM137580, T32GM007198, 5R00CA245187, and 5R00CA245187-04S1), an Allen-AHA Initiative in Brain Health and Cognitive Impairment award (19PABH134610000H), a National Science Foundation NeuroNex Award (2014862), Chan-Zuckerberg Initiative Imaging Scientist Award, the LIFE Foundation, a George E. Hewitt Foundation for Medical Research Postdoctoral Fellowship, Paul F. Glenn Foundation for Medical Research Postdoctoral Fellowship, Salk Pioneer Fund Postdoctoral Scholar Award, the Waitt Foundation, Yale University School of Medicine Center for Cellular and Molecular Imaging, a Canada Research Chair (Tier 2) in Neurobiology of Aging and Cognition, and a Canada Foundation for Innovation John R. Evans Leaders Fund (grant 39965).\r\n<\/p>","protected":false},"featured_media":47466,"template":"","faculty":[308],"disease-research":[333,146,122,176],"class_list":["post-47269","disclosure","type-disclosure","status-publish","has-post-thumbnail","hentry","faculty-gerald-shadel","disease-research-genetics","disease-research-aging-and-regenerative-medicine","disease-research-immune-system-biology","disease-research-mitochondrial-disease"],"acf":[],"yoast_head":"\nFaulty DNA disposal system causes inflammation - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/de\/news-release\/faulty-dna-disposal-system-causes-inflammation\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Faulty DNA disposal system causes inflammation - Salk Institute for Biological Studies\" \/>\n<meta property=\"og:description\" content=\"LA JOLLA\u2014Cells in the human body contain power-generating mitochondria, each with their own mtDNA\u2014a unique set of genetic instructions entirely separate from the cell\u2019s nuclear DNA that mitochondria use to create life-giving energy. 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