{"id":2393,"date":"2012-09-18T00:00:00","date_gmt":"2012-09-18T07:00:00","guid":{"rendered":"https:\/\/vermont.salk.edu\/news-release\/discovery-of-reprogramming-signature-may-help-overcome-barriers-to-stem-cell-based-regenerative-medicine\/"},"modified":"2012-09-18T00:00:00","modified_gmt":"2012-09-18T07:00:00","slug":"discovery-of-reprogramming-signature-may-help-overcome-barriers-to-stem-cell-based-regenerative-medicine","status":"publish","type":"disclosure","link":"https:\/\/www.salk.edu\/de\/news-release\/discovery-of-reprogramming-signature-may-help-overcome-barriers-to-stem-cell-based-regenerative-medicine\/","title":{"rendered":"Discovery of reprogramming signature may help overcome barriers to stem cell-based regenerative medicine"},"content":{"rendered":"<p>LA JOLLA, CA\u2014Salk scientists have identified a unique molecular signature in induced pluripotent stem cells (iPSCs), &#8220;reprogrammed&#8221; cells that show great promise in regenerative medicine thanks to their ability to generate a range of body tissues.\n <\/p>\n<p>\nIn this week&#8217;s <em>Verhandlungen der Nationalen Akademie der Wissenschaften<\/em>, the Salk scientists and their collaborators at University of California, San Diego, report that there is a consistent, signature difference between embryonic and induced pluripotent stem cells. The findings could help overcome hurdles to using the induced stem cells in regenerative medicine.<\/p>\n<div class=\"imageCaption\"><img decoding=\"async\" alt=\"Stem Cell\" src=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2012\/01\/580_Ruiz-StemCell.jpg\"\/><\/p>\n<p>\nA colony of induced pluripotent stem cells. Blue fluorescence indicates cell nuclei; red and green are markers of pluripotency.\n<\/p>\n<p>Bild: Mit freundlicher Genehmigung des Salk Institute for Biological Studies<\/p>\n<\/div>\n<p>\n&#8220;We believe that iPSCs hold a great potential for the treatment of human patients,&#8221; says <a href=\"\/de\/faculty\/belmonte.html\/\">Juan Carlos Izpisua Belmonte<\/a>, a professor in Salk&#8217;s <a href=\"\/de\/faculty\/gene_expression_laboratory.html\/\">Genexpressionslabor<\/a> and the senior author on the paper. &#8220;Yet we must thoroughly understand the molecular mechanisms governing their safety profile in order to be confident of their function in the human body. With the discovery of these small, yet apparent, epigenetic differences, we believe that we are now one step closer to that goal.&#8221;\n <\/p>\n<p>\nEmbryonic stem cells (ESCs) are known for their &#8220;pluripotency,&#8221; the ability to differentiate into nearly any cell in the body. Because of this ability, it has long been thought that ESCs would be ideal to customize for therapeutic uses. However, when ESCs mature into specific cell types, and are then transplanted into a patient, they may elicit immune responses, potentially causing the patient to reject the cells.\n <\/p>\n<p>\nIn 2006, scientists discovered how to revert mature cells, which had already differentiated into particular cell types, such as skin cells or hair cells, back into a pluripotent state. These &#8220;induced pluripotent stem cells&#8221; (iPSCs), which could be developed from the patient&#8217;s own cells, would theoretically carry no risk of immune rejection.\n <\/p>\n<p>\nHowever, scientists found that iPSCs had molecular differences from embryonic stem cells. Specifically, there were epigenetic changes, chemical modifications in DNA that might alter genetic activity. At certain points in the iPSC&#8217;s genome, scientists could see the presence of different patterns of methyl groups when compared to the genomes of ESCs. It seemed these changes occurred randomly.\n <\/p>\n<p>\nIzpisua Belmonte and his colleagues wanted to understand more about these differences. Were they truly random, or was there a discernable pattern?\n <\/p>\n<p>\nUnlike previous studies, which had primarily analyzed iPSCs derived from only one mature type of cells (mainly connective tissue cells called fibroblasts), the Salk and UCSD researchers examined iPSCs derived from six different mature cell types to see if there were any commonalities. They discovered that while there were hundreds of unpredictable changes, there were some that remained consistent across the cell types: the same nine genes were associated with these common changes in all iPSCs.\n <\/p>\n<div class=\"imageCaption530\"><img decoding=\"async\" alt=\"Sergio Ruiz and Juan Carlos Izpisua Belmonte\" src=\"https:\/\/www.salk.edu\/wp-content\/uploads\/2012\/01\/580-Ruiz-Belmontea.jpg\"\/><\/p>\n<p>\nSergio Ruiz, research associate and Juan Carlos Izpisua Belmonte, Professor, Gene Expression Laboratory.\n<\/p>\n<p>Bild: Mit freundlicher Genehmigung des Salk Institute for Biological Studies<\/p>\n<\/div>\n<p>\n&#8220;We knew there were differences between iPSCs and ESCs,&#8221; says Sergio Ruiz, first author of the paper, &#8220;We now have an identifying mark for what they are.&#8221;\n <\/p>\n<p>\nThe therapeutic significance of these nine genes awaits further research. The importance of the current study is that it gives stem cells researchers a new and more precise understanding of iPSCs.\n <\/p>\n<p>\nOther researches on the study were: Dinh Diep (co-first author), Athurva Gore, Athanasia D. Panopoulos, Nuria Montserrat, Nongluk Plongthongkum, Sachin Kumar, Ho-Lim Fung, Alessandra Giorgetti, Josipa Bilic, Erika M. Batchelder, Holm Zaehres, Natalia G. Kan, Hans R. Sch\u00f6ler, Mark Mercola and Kun Zhang.\n <\/p>\n<p>\nThe work was supported by grants from the Instituto de Salud Carlos III, the Focht-Powell Fellowship, Fundacion Cellex, MINECO, <a href=\"http:\/\/www.sanofi.us\/l\/us\/en\/index.jsp\">Sanofi<\/a>, the G. Harold and <a href=\"http:\/\/www.mathersfoundation.org\/\" target=\"_blank\">Leila Y. Mathers Charitable Foundation<\/a>, <a href=\"http:\/\/helmsleytrust.org\/\">Die Leona M. und Harry B. Helmsley Charitable Trust<\/a>, <a href=\"http:\/\/www.cirm.ca.gov\/\">CIRM<\/a>  und <a href=\"http:\/\/www.nih.gov\/\">NIH<\/a>.\n<\/p>\n<p><strong><br \/>\n\u00dcber das Salk Institute for Biological Studies:<\/strong><br \/>\nDas Salk Institute for Biological Studies ist eine der weltweit f\u00fchrenden Institutionen f\u00fcr Grundlagenforschung, an der international renommierte Fakult\u00e4tsmitglieder grundlegende Fragen der Biowissenschaften in einem einzigartigen, kollaborativen und kreativen Umfeld untersuchen. Mit dem Fokus auf Entdeckungen und die Ausbildung zuk\u00fcnftiger Forschergenerationen leisten Salk-Wissenschaftler bahnbrechende Beitr\u00e4ge zu unserem Verst\u00e4ndnis von Krebs, Alterung, Alzheimer, Diabetes und Infektionskrankheiten durch die Untersuchung von Neurowissenschaften, Genetik, Zell- und Pflanzenbiologie sowie verwandten Disziplinen.\n<\/p>\n<p>\nDie Leistungen der Fakult\u00e4t wurden mit zahlreichen Auszeichnungen gew\u00fcrdigt, darunter Nobelpreise und Mitgliedschaften in der National Academy of Sciences. Das 1960 vom Polio-Impfstoff-Pionier Dr. Jonas Salk gegr\u00fcndete Institut ist eine unabh\u00e4ngige gemeinn\u00fctzige Organisation und ein architektonisches Wahrzeichen.<\/p>","protected":false},"featured_media":0,"template":"","faculty":[],"disease-research":[146],"class_list":["post-2393","disclosure","type-disclosure","status-publish","hentry","disease-research-aging-and-regenerative-medicine"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Discovery of reprogramming signature may help overcome barriers to stem cell-based regenerative medicine - Salk Institute for Biological Studies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.salk.edu\/de\/news-release\/discovery-of-reprogramming-signature-may-help-overcome-barriers-to-stem-cell-based-regenerative-medicine\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Discovery of reprogramming signature may help overcome barriers to stem cell-based regenerative medicine - 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