\nTo the host cell, invading viral DNA looks just like the product of DNA damage, which must be repaired or removed in order for the cell to stay healthy. As a result, DNA “security guards” continuously patrol our cells looking for unusual bits of DNA. “We reasoned that viral DNA would be recognized by the cell’s DNA repair machinery and that the virus must somehow manipulate the cell’s response to this foreign DNA,” explains Weitzman.\n<\/p>\n
\nTo test this hypothesis, Weitzman and his team looked at what happens in a virus-infected cell when its DNA is damaged. In a normal cell, DNA damage sensor proteins rush to the site of damage. In cells infected with HSV, however, the cells’ emergency repair teams don’t respond correctly. “The virus effectively overrides the cell’s DNA damage response in order to prevent its own DNA from being recognized,” says Weitzman.\n<\/p>\n
\nThe team went on to identify a single viral protein that is to blame for knocking out the cell’s security system, a protein called ICP0. They discovered that it flags for destruction two important DNA “security guards,” the proteins called RNF8 and RNF168, thereby taking out the DNA damage response in human cells in one big swipe.\n<\/p>\n
\nICP0 attaches so-called ubiquitin marks, which instruct the cell to get rid of the very proteins that protect it. With RNF8 and RNF168 safely out of the way, the virus can begin to take over.\n<\/p>\n
\nDelving deeper, the team looked at the role of these DNA “security guards” that are singled out by ICP0. Surprisingly, RNF8 and RNF168 also leave ubiquitin tags, but in this case, they mark regions of damage. They tag a protein called histone H2A, which directs DNA damage response proteins to accumulate at the sites of damage. The Salk team discovered that by removing RNF8 and RNF168, the viral ICP0 protein results in a decrease to the tag on the cellular H2A protein.\n<\/p>\n
\n“We found that HSV targets the mark that is required to keep DNA damage sensors at damage sites,” says postdoctoral researcher and first author Caroline Lilley, Ph.D. “We now think that HSV deliberately removes this mark so that the virus can infect cells without any trouble from its new host.”\n<\/p>\n
![\"Weitzmann](\"https:\/\/www.salk.edu\/wp-content\/uploads\/2015\/03\/404LabShot.jpg\")
<\/p>\n
Mira Chaurushiya, Matt Weitzman and Caroline Lilley.<\/p>\n<\/div>\n
\nThe findings highlight the importance of these histone marks in DNA damage. “By identifying how HSV dismantles the host’s defense systems, we are shown the key steps, not only in viral infections, but also in the human DNA damage response,” Weitzman explains.\n<\/p>\n
\nHSV may have evolved this weapon because our cells use histone ubiquitination to try to silence gene expression from the viral DNA. “Ubiquitination would be a great way for the cell to silence the viral genome, and ICP0 provides the counterattack by the virus, so the virus and the cell are battling it out at that point,” says Mira Chaurushiya, a graduate student in the lab and contributor to the study.\n<\/p>\n
\nThis work may point to a general mechanism viruses use to overcome the cell’s defense. “DNA damage signaling and ubiquitination may be part of an anti-viral defense mechanism,” explains Lilley. “Part of the cell’s defense is to try to silence viral genomes, and viral proteins have to prevent this in order to achieve infection.”\n<\/p>\n
\nAlong with Weitzman, Lilley and Chaurushiya, other contributors to this work were Sebastien Landry and Junghae Suh from the Salk Institute’s Laboratory of Genetics (J.S. is now at Rice University, Houston); Stephanie Panier and Daniel Durocher from the Samuel Lunenfeld Research Institute, Mount Sinai Hospital in Toronto, Canada; Chris Boutell and Roger D. Everett at the University of Glasgow, UK; and Grant S. Stewart at Birmingham University, UK.\n<\/p>\n
\n-Claire Attwooll<\/em>\n<\/p>\n
\nAbout the Salk Institute for Biological Studies<\/strong>
\nThe Salk Institute for Biological Studies is one of the world’s preeminent basic research institutions, where internationally renowned faculty probe fundamental life science questions in a unique, collaborative, and creative environment. Focused both on discovery and on mentoring future generations of researchers, Salk scientists make groundbreaking contributions to our understanding of cancer, aging, Alzheimer’s, diabetes, and cardiovascular disorders by studying neuroscience, genetics, cell and plant biology, and related disciplines.\n<\/p>\n\nDie Leistungen der Fakult\u00e4t wurden mit zahlreichen Auszeichnungen gew\u00fcrdigt, darunter Nobelpreise und Mitgliedschaften in der National Academy of Sciences. Das 1960 vom Polio-Impfstoff-Pionier Dr. Jonas Salk gegr\u00fcndete Institut ist eine unabh\u00e4ngige gemeinn\u00fctzige Organisation und ein architektonisches Wahrzeichen.<\/p>","protected":false},"featured_media":0,"template":"","faculty":[],"disease-research":[],"class_list":["post-2017","disclosure","type-disclosure","status-publish","hentry"],"acf":[],"yoast_head":"\n
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<\/p>\n