Genome Editing (CRISPR) Events Identification Info
- Ultra-Sensitive Quantification of Genome EditingEvents Using Droplet Digital™ PCR
- Isolation of single-base genome-edited humaniPS cells without antibiotic selection
Prior to sample preparation and submission, please contact Nasun Hah, PhD.
For bioinformatics data analysis advice, please contact Salk Bioinformatics (IGC Core) or visit http://www.salk.edu/science/core-facilities/integrative-genomics-and-bioinformatics-core/
Download appropriate submission forms and fill out completely according to instructions on the first sheet.
|Type of Library||Concentration (determined by Qubit)||Volume||QC (determined by Tape Station or Bioanalyzer)|
|Non-Stranded mRNA (PolyA-selected)||8 ng/uL||100 uL||RIN* > 7|
|Stranded mRNA (PolyA-selected)||8 ng/uL||100 uL||RIN > 7|
|Total RNA (Ribosomal & Mitochondrial RNA depleted)||40 ng/uL||20 uL||RIN > 7|
|Small RNA||170 ng/uL||15 uL||RIN > 7|
|Whole Genome||40 ng/uL||120 uL||>60KB|
|Mouse Exome||40 ng/uL||120 uL||>60KB|
|Human Exome||10 ng/uL||15 uL||>60KB|
|ChIP, Fragmented DNA||1 ng/uL||110 uL||100-800bp|
*RNA Integrity Number (RNA quality, ranging from 0 (degraded) to 10 (perfect); calculated by TapeStation or Bioanalyzer software)