Next Generation Sequencing Core

Services

Salk Institute for Biological Studies - Next Generation Sequencing Core - Services

Services


Sample submission

Prior to sample preparation and submission, please contact Nasun Hah, PhD.

For bioinformatics data analysis advice, please contact Salk Bioinformatics (IGC Core) or visit http://www.salk.edu/science/core-facilities/integrative-genomics-and-bioinformatics-core/

Download appropriate submission forms and fill out completely according to instructions on the first sheet.

Email completed Sample Forms to SampleSubmission@Salk.edu and cc Nasun Hah, PhD.

Bio-Rad QX200 Droplet Digital PCR System Resources

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RNA-Seq Samples Submission Guidelines

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DNA-Seq Samples Submission Guidelines

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Sample requirements

Type of Library Concentration (determined by Qubit) Volume QC (determined by Tape Station or Bioanalyzer)
Non-Stranded mRNA (PolyA-selected) 8 ng/uL 100 uL RIN* > 7
Stranded mRNA (PolyA-selected) 8 ng/uL 100 uL RIN > 7
Total RNA (Ribosomal & Mitochondrial RNA depleted) 40 ng/uL 20 uL RIN > 7
Small RNA 170 ng/uL 15 uL RIN > 7
Whole Genome 40 ng/uL 120 uL >60KB
Mouse Exome 40 ng/uL 120 uL >60KB
Human Exome 10 ng/uL 15 uL >60KB
ChIP, Fragmented DNA 1 ng/uL 110 uL 100-800bp

*RNA Integrity Number (RNA quality, ranging from 0 (degraded) to 10 (perfect); calculated by TapeStation or Bioanalyzer software)