Next Generation Sequencing Core

Scheduling & Rates

Salk Institute for Biological Studies - Next Generation Sequencing Core - Scheduling & Rates

Scheduling & Rates


 

 

Suggested

Library Prep

Price

Run Type

Reads (Millions)

Non-stranded mRNA

$146

SE50

20-30

Stranded mRNA

$136

SE50

20-30

Total RNA-Seq

$200

SE50

25-40

Splice detection

$136

PE125

40-60

Fusion detection

$136

PE125

100-200

Small RNA

$155

SE50

5-10

Whole Genome

$89

PE125

30x coverage

Mouse Exome

$629

PE125

30x coverage

Human Exome

$453

PE125

30x coverage

ChIP-seq

$78

SE50

10-40

For other library prep types, please contact: nhah@salk.edu.

HiSeq 2500

Run type

Price Per Lane*

SE50

$991

SE100

$1262

PE50

$1526

PE125

$2140

*HiSeq 2500 (v4): ~180-250 million reads/lane

NextSeq 500

Run type

Price Per Run^

SE75

$2288

SE150

$3088

PE37

$2288

PE42

$2288

PE75

$3088

PE150

$4420

^NextSeq 500: ~300-400 million reads/run, no wait time for other lanes if submitting enough samples for one run.

                                   
QX200 ddPCR Machine Use Only Machine Use & Reagents**

8 samples

$20

$50

Full plate discount

$220

$300

**QX200 ddPCR reagents include cartridges, gaskets, foil heat seals, semi-skirted 96-well plates, supermix (Evagreen or probes), droplet generation oil for Evagreen or probes, buffer control for Evagreen or probes (if needed), and droplet reader oil. Machine use only researchers must supply their own reagents as listed above.

Other Services

Price Per Sample

Self Service

Full Service

Covaris Sonication

$5

$10

Epishear Sonication

$2

Agilent Tape Station

$7

$15

Qubit Quantitation

$2

$5

Library QC

 –

$20

Library Pooling

 –

$10

Library qPCR Quants

 –

$20

Primer Dimer Cleanup

$20