Next Generation Sequencing Core

Scheduling & Rates

Salk Institute for Biological Studies - Next Generation Sequencing Core - Scheduling & Rates

Scheduling & Rates


 

 

Suggested

Library Prep

Salk Price

Run Type

Reads (Millions)

Stranded mRNA-Seq

$136

1x50bp

20-30

Total RNA-Seq

$200

1x50bp

25-40

Small RNA-Seq

$155

1x50bp

5-10

Whole Genome

$89

2x125bp

30x coverage

Mouse Exome

$629

2x125bp

30x coverage

Human Exome

$453

2x125bp

30x coverage

ChIP-Seq

$78

1x50bp

10-40

ATAC-Seq

Please Inquire

 

15-40

GRO/PRO-Seq

Please Inquire

 

25-40

10X Genomics

Please Inquire

 

 

Nextera XT

Please Inquire

 

 

For other library types, please contact: Nasun Hah nhah@salk.edu.

HiSeq 2500 (v4)

*~180-250 million reads/lane

Run type

Price Per Lane

1x 50 bp

$991

1x 100 bp

$1,262

2x 50 bp

$1,526

2x 125 bp

$2,140


NextSeq 500

*Total ~300-400 million reads

Run type

Price Per Run

1x 75 bp

$2,288

1x 150 bp

$3,088

1x 150 bp (Mid-Output)

$3,088

2 x 37 bp

$2,288

2 x 42 bp (Single index only)

$2,288

2 x 75 bp (Mid-Output)

$1,633

2 x 75 bp

$3,088

2 x 150 bp (Mid-Output)

$2,232

2 x 150 bp

$4,720

For other run configurations, please contact: Nasun Hah nhah@salk.edu.

10X Genomic Single Cell Solution

Machine Usage Per Use

Please Inquire

Additional Cost Per Sample

Please Inquire

Library Prep per sample

Please Inquire

1.Researchers must supply their 10X Genomics Kits
2.See Service Section for Detail
3.Please inquire Salk Institute Quote

#For any inquiries regarding reagents, scheduling, pricing (Salk Institute Quote for 10X Genomics Kits) and 10X training request, please contact: Nasun Hah

Schedule 10X Genomics: Click here for Calendar

Please request access to calendar.

RT-qPCR

per plate

QuantStudio Q3 96 well plate

$10

QuantStudio Q5 384 well plate

$10

#For any inquiries regarding scheduling, consumables and training request, please contact: Ling Ouyang.

Schedule RT-QPCR: Click here for Calendar

Please request access to calendar.

                                   

QX200 ddPCR

Machine Use Only

Machine Use & Reagents**

8 samples

$20

$50

Full plate discount

$220

$550

**QX200 ddPCR reagents include cartridges, gaskets, foil heat seals, semi-skirted 96-well plates, supermix (Evagreen or probes), droplet generation oil for Evagreen or probes, buffer control for Evagreen or probes (if needed), and droplet reader oil.

Other Services

Price Per Sample

Self Service

Full Service

Covaris Sonication

$5

$10

Epishear Sonication

$2

Agilent Tape Station

$7

$15

Qubit Quantitation

$2

$5

Library QC (Qubit and TapeStation)

 –

$20

Library Pooling†† (Includes QC)

 –

$30

Library qPCR Quants

 –

$20

Primer Dimer Cleanup

 –

$20

TECAN Micro Plate Reader#

 

††Library pooling requires core personnel to perform QC
* Machine use only. Researchers must supply their own reagents and plates.