Scheduling & Rates
10x bench users who do not cancel their reservation within 4hrs. notice will be charged a fee of 25% of originally scheduled time. Please contact ngs@salk.edu with any extenuating circumstances.
For general NGS core issues please email ngs@salk.edu
| Instruments & Services | External Non-profit | External For-profit | |||
| Illumina NovaSeq Sequencing (price per run) | Total Reads | Price per flow cell | (price per lane) | Price per flow cell | (price per lane) |
| NovaSeq 6000 SP-100 cycles | 650-800M | $6,983 | $3,491 | $8,728.13 | $4,364.06 |
| NovaSeq 6000 SP-200 cycles | 650-800M | $9,500 | $4,750 | $11,875.00 | $5,937.50 |
| NovaSeq 6000 SP-300 cycles | 650-800M | $11,144 | $5,572 | $13,929.38 | $6,964.69 |
| NovaSeq 6000 SP-500 cycles | 650-800M | $15,162 | $7,581 | $18,952.50 | $9,476.25 |
| NovaSeq 6000 S1-100 cycles | 1.3-1.6B | $10,830 | $5,415 | $13,537.50 | $6,768.75 |
| NovaSeq 6000 S1-200 cycles | 1.3-1.6B | $14,592 | $7,296 | $18,240.00 | $9,120.00 |
| NovaSeq 6000 S1-300 cycles | 1.3-1.6B | $17,214 | $8,607 | $21,517.50 | $10,758.75 |
| NovaSeq 6000 S2-100 cycles | 3.3B-4.1B | $20,121 | $10,061 | $25,151.25 | $12,575.63 |
| NovaSeq 6000 S2-200 cycles | 3.3B-4.1B | $26,885 | $13,443 | $33,606.25 | $16,803.13 |
| NovaSeq 6000 S2-300 cycles | 3.3B-4.1B | $31,084 | $15,542 | $38,855.00 | $19,427.50 |
| NovaSeq 6000 S4-200 cycles | 8-10B | $43,586 | $10,897 | $54,482.50 | $13,620.63 |
| NovaSeq 6000 S4-300 cycles | 8-10B | $52,098 | $13,025 | $65,122.50 | $16,280.63 |
| NovaSeq Xp 2-Lane Kit | _ | $665 | $333 | $831.25 | $415.63 |
| NovaSeq Xp 4-Lane Kit | _ | $1,330 | $333 | $1,662.50 | $415.63 |
| Illumina Sequencing (price per run) | |||||
| MiSeq Reagent Kit v3-150 cycles | 22-25M | $2,280 | $2,850.00 | ||
| MiSeq Reagent Kit v3-600 cycles | 22-25M | $3,610 | $4,512.50 | ||
| MiSeq Reagent Micro Kit v2-300 cycles | 4M | $1,140 | $1,425.00 | ||
| MiSeq Reagent Nano Kit v2-300 cycles | 1M | $855 | $1,068.75 | ||
| MiSeq Reagent Nano Kit v2-500 cycles | 1M | $950 | $1,187.50 | ||
| MiniSeq High Output Kit-75 cycles | 22-25M | $2,280 | $2,850.00 | ||
| MiniSeq High Output Kit-150 cycles | 22-25M | $2,470 | $3,087.50 | ||
| MiniSeq High Output Kit-300 cycles | 22-25M | $3,610 | $4,512.50 | ||
| MiniSeq Mid Output Kit-300 cycles | 7-8M | $1,520 | $1,900.00 | ||
| NextSeq 2000 P1 Reagents-100 cycles | 100M | $2,850 | $3,562.50 | ||
| NextSeq 2000 P1 Reagents-300 cycles | 100M | $3,800 | $4,750.00 | ||
| NextSeq 2000 P1 Reagents-600 cycles | 100M | $5,130 | $6,412.50 | ||
| NextSeq 2000 P2 Reagents-100 cycles | 400M | $4,180 | $5,225.00 | ||
| NextSeq 2000 P2 Reagents-200 cycles | 400M | $6,650 | $8,312.50 | ||
| NextSeq 2000 P2 Reagents-300 cycles | 400M | $8,740 | $10,925.00 | ||
| NextSeq 2000 P3 Reagents-50 cycles | 1.1B | $5,700 | $7,125.00 | ||
| NextSeq 2000 P3 Reagents-100 cycles | 1.1B | $8,170 | $10,212.50 | ||
| NextSeq 2000 P3 Reagents-200 cycles | 1.1B | $11,020 | $13,775.00 | ||
| NextSeq 2000 P3 Reagents-300 cycles | 1.1B | $14,820 | $18,525.00 | ||
| Illumina Library Preparation (price per sample) (click for user guide link) | |||||
| TruSeq Stranded mRNA (PolyA+), sample QC included | $589.00 | $736.25 | |||
| TruSeq Stranded Total RNA Gold Library Prep, sample QC included | $475.00 | $593.75 | |||
| TruSeq Stranded Total RNA with Ribo-Zero Plant Library Prep, sample QC included | $475.00 | $593.75 | |||
| Illumina DNA Prep, sample QC inlcuded | $247.00 | $308.75 | |||
| NEBNext Ultra II DNA (ChIP, Amplicon, <400bp) Prep (no fragmentation) | $228.00 | $285.00 | |||
| Takara SMART-seq HT (ultra-low mRNA) Prep (add-on) | $427.50 | $534.38 | |||
| Illumina DNA PCR-free Prep, sample QC included | $247.00 | $308.75 | |||
| PacBio Sequencing (price per SMRT cell) | |||||
| Sequel IIe 8M SMRT cell (~15h movie) | $3,971 | $4,963.75 | |||
| Sequel IIe 8M SMRT cell (~30h movie) | $4,066 | $5,082.50 | |||
| Sequel IIe Machine Use Only (SMRT cell not included) | $551 | $688.75 | |||
| PacBio Library Preparation (price per sample) | |||||
| HMW DNA Template | $1,045.00 | $1,306.25 | |||
| HMW DNA Template Prep (Multiplexing) | $1,064.00 | $1,330.00 | |||
| HiFi Template Prep | $1,320.50 | $1,650.63 | |||
| Amplicon Template Prep | $560.50 | $700.63 | |||
| ISO-Seq Template Prep | $1,045.00 | $1,306.25 | |||
| ISO-Seq cDNA multiplexing | $380.00 | $475.00 | |||
| Sample QC HMW gDNA | $142.50 | $178.13 | |||
| Sample QC Amplicon, RNA, etc. | $38.00 | $47.50 | |||
| Blue Pippin Size Selection | $285.00 | $356.25 | |||
| NanoString Services (reagents not included) | |||||
| nCounter MAX full-service (price per cartridge (12 samples)) | $560.50 | $700.63 | |||
| GeoMx DSP (price per slide) | $1,377.50 | $1,721.88 | |||
| GeoMx DSP dry run (price per slide) | $1,235 | $1,543.75 | |||
| Single-Cell Solutions (price per hour, reagents not included) | |||||
| 10X bench self-service | $95.00 | $118.75 | |||
| 10X Genomics full-service | $104.50 | $130.63 | |||
| 10X Genomics Training/Assistance | $123.50 | $154.38 | |||
| Vizgen MERSCOPE machine use only | $74.10 | $92.63 | |||
| Plasmid DNA Purification (price per sample) | |||||
| Qiagen DNA Miniprep | $9.50 | $11.88 | |||
| Sample Quality Control (price per sample) | |||||
| TapeStation full-service | $28.50 | $35.63 | |||
| TapeStation 4150 self-service, reagents included | $15.20 | $19.00 | |||
| TapeStation 4150 machine use only | $3.80 | $4.75 | |||
| Qubit full-service | $9.50 | $11.88 | |||
| Qubit self-service, reagents included | $5.70 | $7.13 | |||
| Qubit machine use only | $1.90 | $2.38 | |||
| Library pool qPCR quantification | $57 | $71.25 | |||
| Library Clean-up using AMPure beads | $47.50 | $59.38 | |||
| Sample Shearing (price per sample) | |||||
| EpiShear machine use only | $3.80 | $4.75 | |||
| Covaris M220 machine use only | $7.60 | $9.50 | |||
| Covaris M220 self-service, tube included | $19.00 | $23.75 | |||
| Thermocyclers (price per hour) | |||||
| QuantStudio 3 real-time PCR, 96-well, self-service | $38 | $47.50 | |||
| QuantStudio 5 real-time PCR, 384-well, self-service | $38 | $47.50 | |||
| Plate Reader | |||||
| Tecan Infinite 200 PRO, self-service (plate not included) | Free of charge | Free of charge | |||
| Consumables | |||||
| Plate for Tecan Plate Reader (price per plate) | $19 | $23.75 | |||
| AMPureXP beads (price per 5mL) | $123.50 | $154.38 | |||
| SPRIselect beads (price per 5mL) | $142.50 | $178.13 | |||
| PhiX spike-in | $19.00 | $23.75 | |||
| Training (price per hour) | |||||
| Training/Assistance | $104.50 | $130.63 | |||
| Data Analysis (price per hour) | |||||
| Bioinformatics Data Analysis | $237.50 | $296.88 | |||
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