Gene Transfer, Targeting
and Therapeutics Core

NINDS-R24 Resource Page

Salk Institute for Biological Studies - Gene Transfer, Targeting
and Therapeutics Core - NINDS-R24 Resource Page

NINDS-R24 Resource Page


Salk Institute NINDS-R24 Resource Page for:
Neural Circuit Structure and Function Studies using G-Deleted Rabies Viruses

PI: Edward Callaway

SUMMARY:
Understanding how neurological diseases impair brain function (e.g., memory retrieval and the control of movement) requires a detailed understanding of the affected cell types and neural circuits. A modified version of the rabies virus can be used to trace and experimentally manipulate neural circuits, but these cutting-edge reagents are difficult to produce and are not readily available to the neuroscience community. To generate these reagents in a more efficient manner and to encourage the use of this technology by new research groups, we have established a facility to maintain, propagate, distribute, and improve the G-deleted rabies virus system.

FACILITIES:
This NINDS funded resource is supported by the Salk Institute Core Facility for Gene Transfer, Targeting and Therapeutics (GT3 Core). The Core consists of a large 500-ft2 tissue culture (TC) room and a 500-ft2 main laboratory area, which is equipped to meet the requirements of currently offered services. These services include cloning and production of G-deleted rabies vectors, lentiviral vectors, adenoviral vectors, AAV, and VSV vectors. The TC room is equipped with 5 biological safety cabinets (2 designated for BSL2 and 3 for BSL2+ work), 5 TC incubators, an LED fluorescent microscope with camera, ultracentrifuges, and the full compliment of molecular biology and analytical equipment to meet a wide array of viral vector production needs.

PRODUCTS:
The following is a listing of commonly used stock reagents available through this resource.

Name

Addgene #

Serotypes:

Expresses:

 

 

 

 

AAV-EF1a-DIO-HTB
https://www.addgene.org/44187/

44187

AAV1, AAV2, AAV8, AAVDJ

Histone-tagged GFP, TVA, Rabies B19G

AAV-EF1a-DIO-HB
https://www.addgene.org/37452/

37452

AAV1, AAV2, AAV8, AAVDJ

Histone-tagged GFP, Rabies B19G

AAV-EF1a-FLEX-GTB
https://www.addgene.org/26198/

26197

AAV1, AAV2, AAV8, AAVDJ

Enhanced GFP, TVA, Rabies B19G

AAV-EF1a-FLEX-GT
https://www.addgene.org/26198/

26198

AAV1, AAV2, AAV8, AAVDJ

Enhanced GFP, TVA

AAV-Ef1a-DIO-oG
https://www.addgene.org/74290/

74290

AAV5, AAV8, AAV9

codon optimized PVG and B19G chimera “Optimized G”

AAV-CAG-FLEX-oG
https://www.addgene.org/74292/

74292

AAV5, AAV8, AAV9

“Optimized G”

AV-Ef1a-DIO-H2B-GFP-2A-oG
https://www.addgene.org/74289/

74289

AAV5, AAV8, AAV9

Histone-tagged GFP, “Optimized G”

AAV-CAG-fDIO-oG
https://www.addgene.org/74291/

74291

AAV5, AAV8, AAV9

Flp dependent expression of “Optimized G”

 

Lentiviral Vectors

Name

Addgene #

Expresses:

 

 

 

LV-pBOB-synP-HTB
https://www.addgene.org/30195/

30195

Histone-tagged GFP, TVA, Rabies B19G

LV-pBOB-synP-HT
https://www.addgene.org/30456/

30456

Histone-tagged GFP, TVA

 

Rabies Viral Vectors

Name

Addgene #

Envelopes:

Expresses:

 

 

 

 

G-Deleted Rabies-eGFP
https://www.addgene.org/32635/

32635

Rabies B19G,
EnvA

Enhanced GFP

G-Deleted Rabies-mCherry
https://www.addgene.org/32636/

32636

Rabies B19G,
EnvA

mCherry

G-Deleted Rabies-ChR2-mCherry
https://www.addgene.org/32646/

32646

Rabies B19G,
EnvA

Channelrhodopsin 2-mCherry Fusion

G-Deleted Rabies GCaMP3-dsRedXpress
https://www.addgene.org/32645/

32645

Rabies B19G,
EnvA

GCaMP3 (calcium indicator), dsRedXpress

G-Deleted Rabies eGFP-Er(T2)CreEr(T2)
https://www.addgene.org/32649/

32649

Rabies B19G,
EnvA

Enhanced GFP, Tamoxifen-inducible Cre

G-Deleted Rabies eGFP-rtTA
https://www.addgene.org/32648/

32648

Rabies B19G,
EnvA

Enhanced GFP, Doxycycline-inducible Reverse tTA

G-Deleted Rabies BFP
https://www.addgene.org/32639/

32639

Rabies B19G,
EnvA

Blue Fluorescent Protein

G-Deleted Rabies FLPo-dsRedXpress
https://www.addgene.org/32650/

32650

Rabies B19G,
EnvA

FLP-recombinase, dsRedXpress

G-Deleted Rabies AlstR-GFP
https://www.addgene.org/32647/

32647

Rabies B19G,
EnvA

Allatostatin Receptor, GFP

G-Deleted Rabies dsRed2
https://www.addgene.org/32638/

32638

Rabies B19G,
EnvA

dsRed2

G-Deleted Rabies Cre-GFP

 

Rabies B19G,
EnvA

Cre recombinase, GFP

G-Deleted Rabies eGFP-ArchT

 

Rabies B19G,
EnvA

Enhanced GFP, Archaerhodopsin

G-Deleted Rabies-GCaMP6m-dsRed

 

Rabies B19G,
EnvA

GCaMP6m (calcium indicator), dsRed

Production of custom: G-deleted rabies, rAAV, as well as lentiviral, retroviral, and adenoviral vectors specific for individual NINDS investigator needs.

ORDERING:
To order specific products and information on rates, please follow the link below:
https://www.salk.edu/science/core-facilities/gene-transfer-targeting-and-therapeutics-core/scheduling-and-rates/

VIRTUAL KITS:
https://www.salk.edu/science/core-facilities/gene-transfer-targeting-and-therapeutics-core/ninds-r24-resource-page/virtual-kits/

ACKNOWLEDGING SUPPORT:
Research reported was supported by the National Institute Of Neurological Disorders And Stroke of the National Institutes of Health under Award Number R24NS092943. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.