Instrumentation

Thermo LTQ-Orbitrap XL

This hybrid instrument consists of a linear ion trap and an electrostatic Fourier transform analyzer and is equipped with a nano-spray source. The instrument affords high sensitivity, in the low femtomole range for peptides, and mass accuracies in the single ppm range. The instrument is used for proteomic analysis of proteins either separated by gel electrophoresis or by two-dimensional chromatography (MudPIT). Precursor masses are determined in the high resolution Orbitrap with <10ppm accuracy. Fragmentation is achieved by collisional or higher energy dissociation (CID or HCD). In addition to proteomic analyses, the instrument is capable of analyzing small intact proteins to obtain accurate masses.

AB Perseptive DE-STR MALDI-TOF-MS

This instrument is capable of analyzing both peptides and proteins to obtain intact molecule masses. For peptides, the intact mass can be determined in the sub-picomole range. Proteins can either be analyzed directly (mass range up to 100 kDa) or after enzymatic digestion to obtain a mass "fingerprint".