Salk Institute
Functional Genomics Core
Affymetrix GeneChip

Affymetrix GeneChip Technology provides a sensitive and reliable technology platform for measuring gene expression levels from tens of thousands of genes simultaneously. Gene Expression profiling allows the monitoring of changes in gene expression within diseased or normal biological states, validation and identification of novel drug targets, assessment of toxicological profiles and other biological assays. GeneChip arrays have become the industry-standard whole-genome expression profiling tool. Below are the most used array types.

GeneChip® 3' Expression

GeneChip® 3' expression arrays interrogate each gene with multiple independent probes, offering the most accurate, robust and reproducible 3'-based, gene-level expression profiling. Affymetrix 3' expression portfolio includes whole-genome catalog arrays from Arabidopsis to zebra fish. Current solutions for human, mouse and rat include:

  • Human Genome U133 Plus 2.0 Array [ link ]
  • Mouse Genome 430 2.0 Array [ link ]
  • Rat Genome 230 2.0 Array [ link ]
  • PrimeView™ Human Gene Expression Array [ link ]

GeneChip® Exon 1.0 ST Array System for Human, Mouse and Rat

The Exon 1.0 ST Array System is part of a complete solution that enables whole-genome, gene-level expression and alternative splicing studies. GeneChip ® Exon Arrays provide the most comprehensive coverage of the genome, including empirically supported and predicted transcribed sequences, enabling the discovery of previously unidentified novel events.

  • GeneChip ® Human Exon 1.0 ST Array [ link ]
  • GeneChip ® Mouse Exon 1.0 ST Array [ link ]
  • GeneChip ® Rat Exon 1.0 ST Array [ link ]

The Gene ST Array

The Gene ST Array is part of a complete solution for gene expression analysis that enables whole-genome, gene-level expression studies for well-characterized genes. The array contains the most up-to-date content of well-annotated genes, and data is analyzed using a simple workflow.

  • GeneChip ® Human Gene ST Array [ link ]
  • GeneChip ® Mouse Gene ST Array [ link ]
  • GeneChip ® Rat Gene ST Array [ link ]

The GeneChip® Human Transcriptome Array 2.0 [ link ]

Human Transcriptome Array 2.0 array provides you with the ability to go beyond gene-level expression profiling by providing the coverage required to accurately detect transcript isoforms within a family of transcripts. This high-resolution array design contains an unprecedented >7.3 million distinct probes covering coding and non-coding transcripts. 70% of the probes on this array cover exons for coding transcripts, and the remaining 30% of probes on the array cover exon-exon splice junctions and non-coding transcripts.

 

 

 

 


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