We offer two levels of service:
- Full Service: Target preparation and Array processing
- Basic Service: Array processing
|Array Type||Full Service||Basic Service|
3' expression array: using The GeneChip® 3' IVT Express Kit:
300 ng of Total RNA in 3 µl water, cDNA synthesis, cRNA synthesis, cRNA cleanup, cRNA fragmentation, prepare hybridization cocktails.
Exon/Gene array: using Ambion® WT Expression Kit:
300 ng total RNA in 3 µl water, first cycle cDNA Synthesis, cRNA synthesis and cleanup, second cycle 1st strand cDNA synthesis, hydrolysis of cRNA and cleanup of ssDNA, ssDNA fragmentation, labeling, prepare hybridization cocktails.
Tiling array: using GeneChip® WT Double-Stranded DNA Terminal Labeling Kit:
User is responsible for Chromatin immunoprecipitation and amplification of immunoprecipitated DNA. Core will fragment dsDNA, label fragmented dsDNA, prepare hybridization cocktails.
All arrays will be hybridized overnight with GeneChip Hybridization Oven, then washed with GeneChip Fluidics Station 450, and scanned with GeneChip Scanner 3000 7G.