Salk Institute
Functional Genomics Core
Service Guide

We offer two levels of service:

  • Full Service: Target preparation and Array processing
  • Basic Service: Array processing

Array TypeFull ServiceBasic Service
3' Expression
Exon
Gene
Tiling Partial
SNP  

Target preparation:

3' expression array: using The GeneChip® 3' IVT Express Kit:
300 ng of Total RNA in 3 µl water, cDNA synthesis, cRNA synthesis, cRNA cleanup, cRNA fragmentation, prepare hybridization cocktails.

Exon/Gene array: using Ambion® WT Expression Kit:
300 ng total RNA in 3 µl water, first cycle cDNA Synthesis, cRNA synthesis and cleanup, second cycle 1st strand cDNA synthesis, hydrolysis of cRNA and cleanup of ssDNA, ssDNA fragmentation, labeling, prepare hybridization cocktails.

Tiling array: using GeneChip® WT Double-Stranded DNA Terminal Labeling Kit:
User is responsible for Chromatin immunoprecipitation and amplification of immunoprecipitated DNA. Core will fragment dsDNA, label fragmented dsDNA, prepare hybridization cocktails.

Array Processing:

All arrays will be hybridized overnight with GeneChip Hybridization Oven, then washed with GeneChip Fluidics Station 450, and scanned with GeneChip Scanner 3000 7G.


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