Real-Time PCR Analysis
The Fluidigm Real-Time PCR Analysis software includes:
- Standard curve view—Allows you to easily calculate the standard curve by identifying dilutions samples
- Delta delta Ct calculation—You can get delta delta Ct in just three easy steps
- Automated threshold setting—Automatically determine the ideal threshold setting for each chip run
- Automatic curve quality score—Quickly and efficiently sorts through the real-time PCR curves and exclude those that do not meet your criteria
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Table and Amplification Curve Views
Results may be easily viewed in table format.
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Genotyping Analysis
The Fluidigm Genotyping Analysis software includes additional features specific to genotyping applications including:
- Automatic cluster calling—A highly accurate cluster calling algorithm that ranks among the best in the industry and automatically increases in accuracy as it processes more samples
- Multiple chip scoring—Combines and scores multiple experiments to decrease the analysis time and improve scoring accuracy for rare genotypes
- Confidence score—Automatically determines the confidence level of cluster calls
- Real time or end point—Allows you to view real-time data to aid in assay design while using end point imaging for high throughput applications
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Allele Map
Automatic cluster calling.
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Digital PCR Analysis
The Fluidigm Digital PCR software includes features for digital PCR.
- Multiplex capability—Up to four reporter dyes can be used per reaction, allowing you to quantitate up to four targets per sample in each reaction
- Copy number variation—The software will automatically apply statistical correction and determine the ratio of your targets of interest
- End-point analysis—Allows you to use end-point image for analysis, greatly increasing the throughput of the system
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Heat Map and Call/No-Call Map
The software automatically counts and reports the number of positives for each reporter dye.
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