Salk Institute
Functional Genomics Core


Real-Time PCR Analysis
The Fluidigm Real-Time PCR Analysis software includes:

  • Standard curve view—Allows you to easily calculate the standard curve by identifying dilutions samples
  • Delta delta Ct calculation—You can get delta delta Ct in just three easy steps
  • Automated threshold setting—Automatically determine the ideal threshold setting for each chip run
  • Automatic curve quality score—Quickly and efficiently sorts through the real-time PCR curves and exclude those that do not meet your criteria

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Table and Amplification Curve Views

Results may be easily viewed in table format.

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Genotyping Analysis
The Fluidigm Genotyping Analysis software includes additional features specific to genotyping applications including:

  • Automatic cluster calling—A highly accurate cluster calling algorithm that ranks among the best in the industry and automatically increases in accuracy as it processes more samples
  • Multiple chip scoring—Combines and scores multiple experiments to decrease the analysis time and improve scoring accuracy for rare genotypes
  • Confidence score—Automatically determines the confidence level of cluster calls
  • Real time or end point—Allows you to view real-time data to aid in assay design while using end point imaging for high throughput applications

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Allele Map

Automatic cluster calling.

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Digital PCR Analysis
The Fluidigm Digital PCR software includes features for digital PCR.

  • Multiplex capability—Up to four reporter dyes can be used per reaction, allowing you to quantitate up to four targets per sample in each reaction
  • Copy number variation—The software will automatically apply statistical correction and determine the ratio of your targets of interest
  • End-point analysis—Allows you to use end-point image for analysis, greatly increasing the throughput of the system

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Heat Map and Call/No-Call Map
The software automatically counts and reports the number of positives for each reporter dye.

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