Services: Please contact firstname.lastname@example.org to schedule the BioMark run.
Please read all the tutorials and make sure you have everything needed to run the chip. You can set up your chip in your lab. or in the Core, but please bring all the consumables if setting up in the core. Following are the most used applications.
Taqman Gene Expression, EvaGreen Gene Expression and Taqman SNP Genotyping: Dynamic Array chips in two forms: 48 x 48 and 96 x 96. The 48 x 48 arrays enable testing of 48 samples against 48 assays (a total of 2304 reactions). The 96 x 96 arrays enable testing of 96 samples against 96 assays (a total of 9216 reactions).
Gene Expression Specific Target Amplification Quick Reference rev C1
48.48 Real-Time PCR Workflow Quick Reference rev E1
96.96 Real-Time PCR Workflow Quick Reference rev C1
48.48 Genotyping Workflow Quick Reference rev E1
96.96 Genotyping Workflow Quick Reference rev C1
Digital PCR: Either Taqman probe-based assays or Fluidigm Slingshot reagents may be used for Digital PCR analysis on the Digital Array chips. These are available in two forms: 48 x 770 and 12 x 765. The 48 x 770 array will disperse 48 reaction mixes into 770 separate reactions each. The 12 x 765 array will disperse 12 reaction mixes into 765 reactions each.
The custom arrays are available for gene expression and genotyping. These are the great costs savings alternative assays to Taqman assay. All assays are ready-to-use with Fluidigm standard protocols.