Salk Institute
Flow Cytometry Core Facility

LSR II

The Becton-Dickinson LSR II is a 5-laser 15-color analytical flow cytometer. The cytometer runs BD FACSDiva acquisition software.

Configuration

Please contact staff if your fluorochome cannot be accommodated by the standard filters shown; we have other filters in our collection.

Laser PMT Dichroic Bandpass Effective
Range
Name Examples of fluors
(non-exhaustive list)
488 A 670LP 692/40 672-712 PerCP PerCP, PerCP-Cy5.5
50mW B 505LP 525/50 500-550 FITC FITC, GFP, YFP
  C - 488/10 483-493 SSC SSC
561 A 750LP 780/60 750-810 PE-Cy7 PE-Cy7
100mW B 685LP 710/50 685-735 PE-Cy5.5 PE-Cy5.5
  C 635LP 660/20 650-670 PE-Cy5 PE-Cy5, PI, 7-AAD, mKate, mPlum
  D 600LP 615/45 600-635 PE-Texas Red PE-TR, PI, TR, Alexa-594, mCherry, mLumin
  E - 582/15 574-590 PE PE, Cy3, Alexa-{546,555,568}, Ds-Red, tdTomato
640 A 750LP 780/60 750-810 APC-Cy7 APC-Cy7
40mW B 685LP 730/45 707-750 Alexa Fluor 700 Alexa-{680,700}
  C - 670/30 655-685 APC APC, Cy5, Alexa-{633,647}
405 A 535LP 560/40 535-580 Pacific Orange Pacific Orange
100mW B 505LP 515/20 505-525 mCFP mCFP, Alexa-430, BV510
  C - 450/50 425-475 Pacific Blue Pacific Blue, BV421
355 A 505LP 525/50 500-550 Indo-1 (Blue) Indo-1 (green[lo-ca])
20mW B - 438/24 426-450 Indo-(Violet) Indo-1 (violet[hi-ca]), DAPI

Get Involved

Sign up for our email newsletter

Fill out my online form.
Contact
Salk Institute for Biological Studies
Street: 10010 N Torrey Pines Rd
City: La Jolla, CA 92037
Email: webrequest@salk.edu
Phone: 858.453.4100
Charity Navigator Rating
  • Salk Twitter
  • Salk LinkedIn
  • Salk Facebook
  • Salk Instagram
  • Salk Google+
  • Salk YouTube
  • Salk RSS Feed
© Copyright 2014 Salk Institute for Biological Studies About Scientists & Research News & Media Events Support