FLP-Mediated Gene Modification in Mammalian Cells, and Compositions and Cells Useful Therefor
Inventors: Geoff Wahl and Stephen O'Gorman
Potential Uses: Gene Expression
Rapid and precise site-specific recombination in mammalian cells
The invention relates to methods for the site-specific recombination of DNA in mammalian cells or host mammalian organisms and is based on the recombination of transfected sequences by FLP, a recombinase derived from Saccharomyces. The invention also relates to novel DNA constructs, as well as compositions, cells and host organisms containing such sequences. FLP has been shown to rapidly and precisely recombine copies of its specific target sequence. FLP can be used to mosaically activate or inactivate transgenes for a variety of therapeutic purposes, as well as for analysis of vertebrate development.
Patent Status: U.S. Patent No. 5,654,182 issued August 5, 1997
U.S. Patent No. 5,677,177 issued October 14, 1997
U.S. Patent No. 5,885,836 issued March 23, 1999
Publications: Science Vol. 251, pp. 1351-1355 (1991)
License Terms: Nonexclusive field of use licenses negotiable
Contact: Robert MacWright, Ph.D., Esq., Director, OTD, 858.453.4100 x1703, rmacwright@salk.edu
