Salk Institute

Technologies Available for Licensing

Alpha-Complementation Viral Fusion Assay

Inventors: Carsten Muenk, Anne Holland and Nathaniel Landau
Potential Uses: Antiviral Drug Discovery, Infection
A rapid and quantitative assay to detect HIV and other viral mediated fusion to screen for inhibitors

Entry is an attractive and promising step in HIV-1 replication to target with small molecules. Unlike current drugs that act mainly at reverse transcription or virus maturation, entry inhibitors protect cells from becoming infected and do not need to penetrate the cell. HIV-1 entry is mediated by the interaction of the viral glycoprotein with CD4 and either of the CC chemokine receptors, CCR5 or CXCR4. Current methods for high throughput screening for coreceptor antagonists have advantages and disadvantages. These assays include ligand-receptor competition assays, or cell based assays for cell-cell fusion, virus entry, or single cycle reporter virus infection. None of the assays directly measures the fusion event , but rather measure a secondary result of fusion. As a result, they pick up numerous false positives that act on irrelevant targets. The alpha complementation fusion assay described in the invention provides a means to directly detect envelope glycoprotein-mediated fusion. Moreover, the assay is much simpler and more rapid than any other method. Also, importantly, the assay does not use live virus and therefore can be performed without biohazard containment. The assay is done in 96 well microtiter plates and is easily adaptable to 384 or 1024 well format. The assay is scalable and easily adapted to high throughput screening. In addition, the assay can be adapted to any enveloped virus.

Salk No: S02016A
Patent Status: U.S. Patent No. 7,312,031 issued December 25, 2007
Publications: Virology 319(2):343-352 (February 2004)
License Terms: Non-exclusive licenses available
Contact: Michelle Booden, Ph.D., Director of Licensing, 858.453.4100 x1612, mbooden@salk.edu

Get Involved

Sign up for our email newsletter

Fill out my online form.
Contact
Salk Institute for Biological Studies
Street: 10010 N Torrey Pines Rd
City: La Jolla, CA 92037
Email: webrequest@salk.edu
Phone: 858.453.4100
Charity Navigator Rating
  • Salk Twitter
  • Salk LinkedIn
  • Salk Facebook
  • Salk Instagram
  • Salk Google+
  • Salk YouTube
  • Salk RSS Feed
© Copyright 2014 Salk Institute for Biological Studies About Scientists & Research News & Media Events Support