Salk Institute
Waitt Advanced Biophotonics Center
Martin W. Hetzer

Martin W. Hetzer

Professor
Faculty Director Waitt Advanced Biophotonics Center
Molecular and Cell Biology Laboratory
Jesse and Caryl Philips Foundation Chair


Cell cycle and cancer

Using time-lapse microscopy, we showed that nuclear envelope (NE) formation is mediated by reshaping of the endoplasmic reticulum and not as previously thought by vesicle fusion. Our results answered a long-standing question in the field of nuclear biology and provided a new paradigm for membrane dynamics.

In continuation of this work we recently discovered a remarkable phenomenon whereby the NE becomes transiently ruptured and repaired during interphase in various human cancer cells. Strikingly, NE rupturing was associated with loss of cell compartmentalization and a catastrophic chromosome rearrangement event called chromothripsis and thus might be a source of genomic instability.

Cell differentiation and development

Using Drosophila genetics in combination with imaging and DNA sequencing methods in mouse and human stem cells, we discovered that several NE proteins play an essential role in transcriptional activation of developmentally regulated genes. These findings provided first evidence for a functional role of NE-mediated gene regulation and establish a new framework for studying the spatial organization of the nuclear genome. Most recently, we could show that the nuclear pore protein Nup153 plays a role in stem cell pluripotency through gene silencing.

Protein homeostasis and aging

Initially using the model organism C. elegans followed by metabolic labeling experiments in rats and quantitative mass spectrometry, we discovered long-lived proteins (LLPs) in the NE and on chromatin, which exhibit no or very little protein turnover in the adult brain. Our results reveal a novel aspect of protein homeostasis in the nucleus and suggest that a failure to maintain proper levels and functional integrity of LLPs could be a major contributor to age-related changes in the function of post-mitotic tissues. We plan to decipher the mechanisms by which the functional integrity of these proteins is protected over long periods of time, and determine whether their eventual functional decline contributes to age-related pathologies in the brain.


Education

  • Undergraduate degree, University of Vienna, Austria
  • PhD, Biochemistry and Genetics, Vienna Biocenter, Austria
  • Postdoctoral work at EMBL, Heidelberg, Germany

Awards and Honors

  • 2013 Glenn Award for Research in Biological Mechanisms of Aging
  • 2009 ASCINA Award (Austrian Scientists and Scholars in North America)
  • Early Career Life Science Award, American Society of Cell Biology 2009
  • Ellison Medical Foundation Senior Scholar in Aging (2009-2013)
  • American Cancer Society Research Scholar (2009-2013)
  • Pew Scholar 2005
  • APART fellow 2000-2003
  • EMBO long term fellowship 1998-2003
  • Austrian science award in Genetics 1997
  • Erwin Schroedinger fellowship 1997

Selected Publications

  • Hatch, E.M. and Hetzer, M.W. (2015) Linking Micronuclei to Chromosome Fragmentation. Cell 161:1502-1504
  • Jacinto, F.V., Benner, C. and Hetzer, M.W. (2015) The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev. 29:1224-1238
  • Hatch, E.M. and Hetzer, M.W. (2015) Chromothripsis. Curr. Biol. 25:R397-399
  • Gomez-Cavazos, J.S. and Hetzer, M.W. (2015) The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis. J. Cell Biol. 208:671-681
  • Ibarra, A. and Hetzer, M.W. (2015) Nuclear pore proteins and the control of genome functions. Genes Dev. 29:337-349.
  • Buchwalter, A.L., Liang, Y. and Hetzer, M.W. (2014) Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics. Mol. Biol. Cell 25:2472-84
  • Hatch, E. and Hetzer, M. (2014) Breaching the nuclear envelope in development and disease. J. Cell Biol. 205:133-41
  • Buchwalter, A. and Hetzer, M.W. (2014) Nuclear pores set the speed limit for mitosis. Cell 156:868-869
  • Toyama, B.H., Savas, J.N., Park, S.K., Harris, M.S., Ingolia, N.T., Yates, J.R. and Hetzer, M.W. (2013) Identification of long-lived proteins reveals exceptional stability of essential cellular structures. Cell 2013 Aug 29;154(5):971-82. doi: 10.1016/j.cell.2013.07.037.
  • Hatch, E.M., Fischer, A.H., Deerink, T.J. and Hetzer, M.W. (2013) Catastrophic nuclear envelope collapse in cancer cell micronuclei. Cell 154:47-60.
  • Regner, B.M, Vucinic, D., Domnisoru, C., Bartol, T.M., Hetzer, M.W., Tartakovsky, D.M. and Sejnowski, T.J. (2013) Characterizing intracellular transport mechanisms with random walks in a model system. Biophys J 104:1652-1660.
  • Liang, Y., Franks, T.M., Marchetto, M.C., Gage, F.H and Hetzer, M.W. (2013) Dynamic association of NUP98 with the human genome. Plos Genet 9:e1003308
  • Toyama, B.H. and Hetzer, M.W (2013) Protein homeostasis: live long won't prosper. Nature Rev Mol Cell Biol 14:55-61.
  • Franks, T.M. and Hetzer, M.W (2013) The role of Nup98 in transcription regulation in healthy and diseased cells. Trends in Cell Biol 23:112-117.
  • Savas, J.N., Toyama, B.H., Xu, T., Yates J.R. and Hetzer, M.W. (2012) Extremely long-lived nuclear pore proteins in the rat brain. Science 335: 942.
  • D'Angelo, M.A., Gomez-Cavazos, J.S., Mei, A., Lackner, D.H. and Hetzer, M.W. (2012) A change in nuclear pore complex composition regulates cell differentiation. Dev Cell 22, 1-13
  • Talamas, J. and Hetzer, M.W. (2011) POM121 and Sun1 play a role in ealry steps of interphase NPC assembly. J Cell Biol 194: 27-37
  • Doucet, C., Talamas, J. and Hetzer, M.W. (2010) Cell Cycle Dependent Differences in Nuclear Pore Complex Assembly in Metazoa. Cell 141: 1030-1041.
  • Capelson, M., Liang, Y., Schulte, R., Mair, W, Wagner, U. and Hetzer, M.W. (2010) Chromatin-bound nuclear pore components regulate gene expression in higher eukaryotes. Cell 140: 372-383.
  • D'Angelo, M.A., Raices, M., Panowski, S.H. and Hetzer, M.W. (2009) Age-dependent deterioration of nuclear pore complexes causes a loss of nuclear integrity in post-mitotic cells. Cell 136: 284-295.

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